CN103931901B - A kind of additive that regulates sea cucumber function of intestinal canal and its preparation method and application - Google Patents
A kind of additive that regulates sea cucumber function of intestinal canal and its preparation method and application Download PDFInfo
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- CN103931901B CN103931901B CN201410164989.XA CN201410164989A CN103931901B CN 103931901 B CN103931901 B CN 103931901B CN 201410164989 A CN201410164989 A CN 201410164989A CN 103931901 B CN103931901 B CN 103931901B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention discloses a kind of additive that regulates sea cucumber function of intestinal canal and its preparation method and application, additive provided by the invention is made up of the component of carrying out by ratio of weight and the number of copies as follows proportioning: montmorillonite 50-70 part, probio 15-25 part, medical stone 20-30 part, this is that one is taking environmental material as main, micro-ecomaterial is auxiliary additive, can effectively make sea cucumber function of intestinal canal keep normal by the proportioning of science, digests and assimilates unaffected; Promote its metabolic function simultaneously, improve the speed of growth, greatly reduced the impact of intestines problem on holothruian cultures; And preparation method of the present invention and using method easy, only additive of the present invention need be added in feed or directly carries out water by consumption at culturing pool and sprinkle according to proportioning.
Description
Technical field
The invention belongs to holothurian feed additive, be specifically related to a kind of additive and preparation thereof that regulates sea cucumber function of intestinal canalMethods and applications.
Background technology
" sea cucumber, someone is referred to as " extra large ginseng ", because the similar ginseng of benefiting action is gained the name. Sea cucumber has 1100 in the whole worldMultiple, there is kind more than 100 in China, wherein the famousst and preciously surely belongs to stichopus japonicus, namely " sea cucumber " of our common indication. Large precious as eightOne of product, it is not only precious food, is also famous and precious medicinal material, shows after deliberation, and sea cucumber has the memory of raising, delayGland aging, prevents artery sclerosis, diabetes and the effect such as antitumor. Along with the raising of people's living standard, the demand of sea cucumberAmount also significantly rises.
Therefore, in recent ten years, propagating artificially gradually of sea cucumber risen, and becomes very soon emerging a, prospect veryWide aquaculture industry. But when sea cucumber is propagated artificially on a large scale, disease problem is increasingly outstanding, occur multiple obviousIllness and the extensive phenomena of mortality, seriously restricted the sound development of this industry. Sea cucumber is benthonic animal, not main to foodMoving selective, mainly, taking benthic diatom, organic debris, various microorganism and planktonic organism as main, send out by the microscopy to diseaseExisting, in lesions position and body, there are the phenomenons such as bacterium erosion and mould, parasitic infection. So a lot of people think onlyControl bacterium and virus, sea cucumber disease will be controlled. A large amount of antibiotic is just included into actual life naturallyIn product process. But due to for a long time, use the chemicalses such as antibiotic in a large number, pathogen also can produce drug resistance, sea cucumber enteron aisle withFlora imbalance in breeding environment, causes sea cucumber immunity degradation, and various diseases recurrence rate is higher, and medicament residue also can endanger simultaneouslyHealth. Therefore researching and developing safe and efficient and additive adjusting sea cucumber enteron aisle that have no side effect has holothruian culturesImportant realistic meaning; Because propagating artificially in sea cucumber process, keep constantly the normal digestion and absorption function of sea cucumber enteron aisle, rightThe growth of sea cucumber is very necessary, and sea cucumber is supported in environment after pathogenic reason is bait and ight soil corruption mostly in pond, the having of generationPoisonous substance matter, the intestines problem causing as ammonia nitrogen, hydrogen sulfide etc.
Summary of the invention
The invention provides one taking environmental material as main for the problems referred to above, micro-ecomaterial is auxiliary adjusting sea cucumber intestineRoad functional additive, makes the function of intestinal canal of sea cucumber keep normal, digests and assimilates unaffectedly, and metabolic function is vigorous.
The additive of adjusting sea cucumber function of intestinal canal of the present invention, according to ratio of weight and number, is made up of following component: coverDe-stone 50-70 part, probio 15-25 part, medical stone 20-30 part.
Probio described in the present invention be a kind of in lactic acid bacteria, saccharomycete, bacillus subtilis, bacillus licheniformis orTwo kinds and two or more mixing.
Preferably, in the present invention the described ecotype regulates the additive of sea cucumber function of intestinal canal, and each composition by weightShared portion rate is respectively: 60 parts of montmorillonites, 20 parts of probios, 25 parts of medical stones.
Preferably, probio described in the present invention: the weight of lactic acid bacteria, saccharomycete, bacillus subtilis, bacillus licheniformisAmount is than being 3-5:2-5:1-2:1-2.
Preferably, in the present invention described montmorillonite is that more than 2 fineness rank is carried out weight proportion group by different purposesBecome, more than fineness scope 180 orders; Make the uneven seed individuality of size in pond all obtain close result of use, so selectFineness scope larger.
Preferably, in the present invention the fineness of described medical stone is more than 200 orders.
In the present invention, the preparation method of the additive of described adjusting sea cucumber function of intestinal canal realizes by following steps: by heavyAmount takes each component, then each component is put into waist drum mixer and the efficient mixing machine of twin shaft mixes, and mixes rear variation linesNumber is less than 5%, and metering packing becomes bag.
In the present invention, the application of the additive of described adjusting sea cucumber function of intestinal canal refers to that described additive is raw in holothruian culturesThe application in product field.
Specifically, the application of the additive of described adjusting sea cucumber function of intestinal canal can be by the following method in the present inventionUse: according to the ratio of 2-5%, this additive is evenly admixed to sea cucumber compound feed, or straight in the sea cucumber energy feed such as seawood mealConnect and throw something and feed, also can carry out water splashing by the dosage of 5-10 gram of/cube of water body.
Described sea cucumber enteron aisle regulates being applied as according to following mode of additive to throw in above-mentioned interpolation in the present inventionAgent: water temperature 18-28 DEG C time, seedling breeding density is 0.2-2KG/ cube of water body, each injected volume of additive and the weight of sea cucumberAmount is than being 1:50-100, and the cycle of falling pond is 6-15 days; Water temperature is in the time of 8-18 DEG C, and seedlings breeding density is 2-5KG/ cube of water body,The injected volume of additive and the weight ratio of sea cucumber are 1:80-300, and the cycle of falling pond is 10-20 days.
Montmorillonite Ex(H2O used in the present invention)4{（Al2-x,Mgx）2[（Si,Al）4O10]（OH）2Claim that again crystallite is highRidge stone, has very strong adsorption capacity and ion-exchange capacity; Also there is colloidality, plasticity and the cohesive force of height simultaneously.It is widely used in medicine, can do medical carrier, plays controlled release agent effect. Montmorillonite has very in domestic animals (pig, rabbit) cultivationGood application, the especially yellowish-white dysentery of son, sucking pig, having loose bowels of rabbit, plays prevention effect, in view of the characteristic of montmorillonite, Hai NengzuoFor the first-selection of de-mould dose of classic feed. Montmorillonite has suction-operated to bacterium, and absorption affinity is strong and weak relevant with its charging property. My godSo montmorillonite, has lamellar structure and heterogeneity CHARGE DISTRIBUTION, to the poison of the virus in alimentary canal, germ and generation thereofElement, gas etc. have extremely strong fixing, inhibitory action, make it lose pathogenic effects; In addition alimentary canal mucous membrane is also had very strongCovering protection ability, repairs, improves the defense function of Mucosa Barrier to attack factor, has balance normal flora and local analgesiaEffect. Montmorillonite has good suction to Escherichia coli, comma bacillus, campylobacter jejuni, golden Portugal bacterium and rotavirus and cholateAttached effect, and effects on surface has fixing scavenging action with the pathogenic charged pathogen of grain encoding proteins (CS31A). Research is summed upAlso show that nano montmorillonite is higher than common montmorillonite adsorption capacity.
In the present invention, medical stone used is a kind of to biological nontoxic, harmless and have certain bioactive complex mineralOr medicinal rock, the main chemical compositions of medical stone is inorganic alumino-silicate. Comprising SiO2、Al2O3、Fe2O3、FeO、MgO、CaO、K2O、Na2O、TiO2、P2O5, MnO etc., also contain animal required macroelement K, Na, Ca, Mg and Cu, Mo etc. micro-Secondary element and rare earth element, approximately 58 kinds more than. In medical stone, containing aluminum silicate (feldspar), check colors and have adsorption capacity, ifBy medical stone pulverize, ion stripping and suction-operated strengthen. Medical stone is a kind of neutral alkali half igneous rock, close to volcanoRock. The natural mineral matter comprising in medical stone is easy to discharge, and from medical stone, in countless aperture, discharges oxygen, floats by absorptionWhite powder and other noxious material are purified waste water. The mineral matters such as the calcium that gives out in medical stone, iron, sodium can be improved drinking water. Medical stoneEffectiveness for feed can make domestic animal become more healthy and stronger. Find by the analysis to medical stone powder and decomposition, medical stone is by 25000Multiple components composition. It can also effectively alleviate excremental smell, contributes to the stalwartness of domestic animal.
Due to the strong adsorptivity of medical stone and the characteristic of released mineral matter, it can be used for activating, purifying waste water and remove waterIn harmful substance. Medical stone can absorb, decompose, remove various materials, as: amine, chlorine, mercury, cadmium, cyanogen, White staphylococcus, knotEnterobacteria (comprising 0-157), bacterium and various germ.
Sea cucumber pond support in environment pathogenic main cause be mostly due to pond at the bottom of after residual bait and ight soil corruption, the having of generationPoisonous substance matter, as the intestines problem that ammonia nitrogen, hydrogen sulfide etc. caused. Effect of the present invention is exactly to control the Toxic such as ammonia nitrogen in pondMatter, removes the germ in enteron aisle, supplements mineral matter and trace element.
In the present invention, probio used mostly derives from alimentary canal and acts on again alimentary canal, can effectively supplement animal digestionBeneficial microbe colony in road, suppresses pathogen growth, improves archenteric flora balance, improves immunity function and the life of sea cucumberLong speed; Mix with holothurian feed by a certain percentage when itself and above-mentioned montmorillonite, medical stone powder are mixed and made into additive, or pressDirectly rendering to according to certain proportion the beneficial effect that culturing pool plays is:
1. the raw material adopting in the present invention can effectively make sea cucumber function of intestinal canal keep normal by the proportioning of science,Digest and assimilate unaffected;
2. promote its metabolic function simultaneously, improve the speed of growth, greatly reduced the shadow of intestines problem to holothruian culturesRing;
3. using method is easy, only additive of the present invention need be added in feed or directly by use according to proportioningAmount is carried out water at culturing pool and is sprinkled;
4. the present invention, can also be by the poisonous germ of absorption, removing sea cucumber excreta in adjusting sea cucumber function of intestinal canalIn harmful substance so that effectively purify water, and can provide sea cucumber grow necessary mineral matter and trace element;
5. the present invention is taking ecomaterial as main, and micro-ecomaterial is auxiliary, is environmental protection raw material, uses of the present inventionProduct can reduce antibiotic use, so improve sea cucumber commercial quality, ensure food safety.
Detailed description of the invention
The montmorillonite of additive Raw in the following embodiments: join meticulous montmorillonite Co., Ltd in Shouguang, Shandong; WheatMeal stone: Good products from the earth are nature's treasures in Chifeng mineral material Co., Ltd; Probio, the industrial Bioisystech Co., Ltd in Cangzhou; Holothruian cultures are specialWith feed: Qingdao Hai Jie Aqua Sciences Inc..
Embodiment 1
Prepare additive: 100kg montmorillonite, 30kg lactic acid bacteria and 40kg medical stone are put into waist drum mixer and twin shaft highEffect mixing machine mixes, and mixes the rear coefficient of variation and is less than 5%, and metering packing becomes bag; Wherein the montmorillonite of 100kg be 20kg200 order,500 orders of 40kg300 order and 40kg mix.
Embodiment 2
Prepare additive: 140kg montmorillonite, 50kg probio and 60kg medical stone are put into waist drum mixer and twin shaft highEffect mixing machine mixes, and mixes the rear coefficient of variation and is less than 5%, and metering packing becomes bag. ; Wherein the montmorillonite of 140kg be 40kg230 order,400 orders and the 20kg500 order of 40kg300 order, 40kg mix; Wherein said probio is saccharomycete and bacillus subtilisBacterium, the proportion relation of the two is 2:1.
Embodiment 3
Prepare additive: 120kg montmorillonite, 40kg probio and 50kg medical stone are put into waist drum mixer and twin shaft highEffect mixing machine mixes, and mixes the rear coefficient of variation and is less than 5%, and metering packing becomes bag; Wherein the montmorillonite of 120kg be 40kg200 order,500 orders of 40kg300 order and 40kg mix; Described probio is lactic acid bacteria and bacillus licheniformis, and the proportioning of the two is closedSystem is 1:1.
Specific experiment data and result
Experiment 1
1) under identical feeding environment: Chi Shui: 40 cubes; 13 DEG C of water temperatures; Carry 450 grams of/cube of water bodys of seedling amount; Change every dayWater 20%, 13 day falls pond; 2 bait throwing in every day, perfect compound feed protein content 24%, is seldom advisable with last residual bait; Other is dailyManage identical;
2) seedling size is 500-2000/jin; The enforcement time is 2 cycles of falling pond; Contrast pond is not for being used the present inventionSeedling, experiment pool is for being used seedling of the present invention (to throw bait every day 2 times, use the additive of the embodiment of the present invention 1 and 2, by 2%Mix bait; Change every day after water by the additive of 5 grams of/cube of water splashing embodiments of the invention 1 and 2 once simultaneously. In experiment, realTest pond 1 corresponding to embodiment 1, experiment pool 2 is corresponding to embodiment 2).
Experiment contrast table 1: on the impact of seedling enteron aisle and growth property
Experiment 2
1) under identical feeding environment: Chi Shui: 40 cubes; 15 DEG C of water temperatures; Carry 1500 grams of/cube of water bodys of seedling amount; Every dayChange water 20%, 13 day and fall pond; 2 bait throwing in every day, perfect compound feed protein content 20%, is seldom advisable with last residual bait; Other dayNormal management is identical;
2) seedling size is 200-500/jin; The enforcement time is 2 cycles of falling pond; Contrast pond is not for being used the present inventionSeedling, in order to use seedling of the present invention, (2 bait throwing in every day are used the additive of the embodiment of the present invention 1 and 3, by 3% to experiment poolMix bait; Change every day after water by the additive of 7 grams of/cube of water splashing embodiments of the invention 1 and 3 once simultaneously. In experiment, realTest pond 1 corresponding to embodiment 1, experiment pool 2 is corresponding to embodiment 3)).
Experiment contrast table 2: on the impact of seedling enteron aisle and growth property
Experiment 3
1) under identical feeding environment: Chi Shui: 40 cubes; 18 DEG C of water temperatures; Carry 2500 grams of/cube of water bodys of seedling amount; Every dayChange water 20%, 13 day and fall pond; 2 bait throwing in every day, perfect compound feed protein content 17%, is seldom advisable with last residual bait; Other dayNormal management is identical;
2) seedling size is 50-200/jin; The enforcement time is 2 cycles of falling pond; Contrast pond is of the present invention for not usingSeedling, in order to use seedling of the present invention, (2 bait throwing in every day, the additive of the use embodiment of the present invention 2 and 3, mixes by 4% experiment poolBait; Change every day after water by the additive of 8 grams of/cube of water splashing embodiments of the invention 2 and 3 once simultaneously. In experiment, experimentPond 1 is corresponding to embodiment 2, and experiment pool 2 is corresponding to embodiment 3).
Experiment contrast table 3: on the impact of seedling enteron aisle and growth property
Special instruction:
Above three groups of experiments, whenever approaching time of falling pond for the second time, are not used seedling in the contrast pond of this additive, except growingAlso all having there is intoxicating phenomenon outside obviously lagging behind in speed, such as deadlock is black, shrink, apocleisis etc., also has a little seedling to go out in various degreeShowed infectious diseases sign, in experiment pool, seedling is without this phenomenon, because this is not this experiment main contents, therefore do not have list detailedContrast.
Show by the mix fodder holothruian cultures experimental data of throwing something and feeding or directly throw something and feed: at actual holothruian cultures ringIn border, use additive of the present invention effectively to improve the function of intestinal canal of sea cucumber, promote its metabolic function simultaneously, improve growthSpeed, especially, within the cycle of falling pond for the second time, the present invention is very large on sea cucumber enteron stool situation and growth rate impact; ToolBody is exactly additive of the present invention in immunity and enteron aisle digestion power by strengthening sea cucumber, has improved seaThe premunition of ginseng, and can effectively improve water quality, be of great significance improving cultivation survival rate and product quality tool.
The above, be only preferably detailed description of the invention of the present invention, but protection scope of the present invention is not limited to this,Anyly be familiar with in technical scope that those skilled in the art disclose in the present invention, according to technical scheme of the present invention andInventive concept is equal to replaces or changes, within all should being encompassed in protection scope of the present invention.
Claims (7)
1. an additive that regulates sea cucumber function of intestinal canal, is characterized in that, is made up of montmorillonite, probio and medical stone, eachIndividual composition by weight shared portion rate is respectively: montmorillonite 50-70 part, probio 15-25 part, medical stone 20-30 part;
Described probio is one or more mixed in lactic acid bacteria, saccharomycete, bacillus subtilis, bacillus licheniformisClose; Described a kind of preparation method who regulates sea cucumber enteron aisle functional additive: take by weight each component, then by each componentPut into the efficient mixing machine of waist drum mixer and twin shaft and mix, mix the rear coefficient of variation and be less than 5%, then metering packing becomes bag.
2. a kind of additive that regulates sea cucumber function of intestinal canal according to claim 1, is characterized in that, each component is by heavyMeasuring shared portion rate is respectively: 60 parts of montmorillonites, 20 parts of probios, 25 parts of medical stones.
3. a kind of additive that regulates sea cucumber function of intestinal canal according to claim 1 and 2, is characterized in that, described illiteracy is de-The fineness of stone is made up of more than 2 fineness rank proportioning, and fineness scope is more than 180 orders.
4. a kind of additive that regulates sea cucumber function of intestinal canal according to claim 1 and 2, is characterized in that, described wheat mealThe fineness of stone is more than 200 orders.
5. a kind of additive that regulates sea cucumber function of intestinal canal according to claim 1, is characterized in that, described probio breastThe weight ratio of acid bacterium, saccharomycete, bacillus subtilis, bacillus licheniformis is 3-5:2-5:1-2:1-2.
6. described in above-mentioned arbitrary claim, additive, in the purposes of preparing in holothurian feed, is characterized in that described additiveApply at holothruian cultures production field.
7. purposes according to claim 6, is characterized in that, described additive uses by the following method: according to 2-5%Ratio described additive is evenly admixed in sea cucumber compound feed or sea cucumber energy feed and is is directly thrown something and fed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410164989.XA CN103931901B (en) | 2014-04-23 | 2014-04-23 | A kind of additive that regulates sea cucumber function of intestinal canal and its preparation method and application |
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| CN201410164989.XA CN103931901B (en) | 2014-04-23 | 2014-04-23 | A kind of additive that regulates sea cucumber function of intestinal canal and its preparation method and application |
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| CN103931901A CN103931901A (en) | 2014-07-23 |
| CN103931901B true CN103931901B (en) | 2016-05-25 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106889374A (en) * | 2017-02-20 | 2017-06-27 | 辽宁天牧生物科技有限公司 | A kind of sea cucumber granules feed rich in various probiotics |
| CN111616259A (en) * | 2020-06-02 | 2020-09-04 | 沈阳博善英胜生物技术有限公司 | Production method of fermented dry feed capable of fully playing material adsorption role |
| CN113402035B (en) * | 2021-06-16 | 2023-04-07 | 苏州大腾生态科技发展有限公司 | Green modifier for landscape/aquaculture water body and preparation method and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1709135A (en) * | 2004-09-16 | 2005-12-21 | 青岛金海力水产科技有限公司 | Compound fodder for holothurian culture |
| CN103005216A (en) * | 2012-12-28 | 2013-04-03 | 青岛七好生物科技有限公司 | Sea mud substitute for adult stichopus japonicus feeds |
| CN103461677A (en) * | 2013-09-25 | 2013-12-25 | 南京日升昌生物技术有限公司 | Mold removal agent for animal feed as well as preparation method and application of mold removal agent |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1709135A (en) * | 2004-09-16 | 2005-12-21 | 青岛金海力水产科技有限公司 | Compound fodder for holothurian culture |
| CN103005216A (en) * | 2012-12-28 | 2013-04-03 | 青岛七好生物科技有限公司 | Sea mud substitute for adult stichopus japonicus feeds |
| CN103461677A (en) * | 2013-09-25 | 2013-12-25 | 南京日升昌生物技术有限公司 | Mold removal agent for animal feed as well as preparation method and application of mold removal agent |
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