CN104068217A - Anti-mycotoxin feed additive for cattle - Google Patents
Anti-mycotoxin feed additive for cattle Download PDFInfo
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- CN104068217A CN104068217A CN201410259666.9A CN201410259666A CN104068217A CN 104068217 A CN104068217 A CN 104068217A CN 201410259666 A CN201410259666 A CN 201410259666A CN 104068217 A CN104068217 A CN 104068217A
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Abstract
The invention discloses an anti-mycotoxin feed additive for cattle. The anti-mycotoxin feed additive is prepared from the following raw materials in parts by weight: 10-14 parts of scutellaria baicalensis, 8-12 parts of syzygium aromaticum, 8-12 parts of mint, 12-18 parts of bamboo leaves, 1-5 parts of tea polyphenol, 3-8 parts of sodium diacetate, 30-50 parts of nano montmorillonite and 8-25 parts of active saccharomyces cerevisiae. The anti-mycotoxin feed additive can be used for effectively adsorbing mycotoxin, preventing cattle feed from being moldy, effectively promoting an organism to defend the absorption of mycotoxin, enhancing the immune capability of the organism, adjusting gastrointestinal functions of beef cattle and promoting the absorption of nutrient substances, and has the effects of invigorating stomach and helping to digest as well as promoting absorption and growth.
Description
Technical field
A kind of feed addictive of the present invention, particularly antifungi toxin feed addictive for a kind of ox.
Background technology
Mycotoxin be crops or animal protein because of the toxic metabolite product that mould produces, it has been found that at present hundreds of mycotoxins, these toxin have different toxicity, metabolic pathway and target organ in animal body.The harm that mycotoxin causes is a global problem, is present in nearly all feedstuff and human food's raw material.The pollution of Mycotoxins in Feed and the harm causing thereof are still the problem that culturist is easy to ignore at present, and easily obscure with Other diseases generation.According to FAO (Food and Agriculture Organization of the United Nation), estimate, the agricultural product such as the grain in the whole world annual nearly 5%～7%, feed suffer the erosion of mould.
Mould is a kind of many cells microorganism, and it is multiplied lower generation by seed or embraces sub form.Mould lake is prevalent in soil and some rotted plant materials.Mould in soil embrace son via air, water and entomochory to plant, once lake touches the seed breaking, embrace son and send out before rapidly, just can obviously see mouldy phenomenon.These moulds multiply and can remove to infect other seed by the more son of embracing of generation.In field, plant is subject to the factor of mycotic infection a lot, comprising: the moisture of soil, sowing time, harvest time.The kind of crop rotation phase, fertilising, plant, plant the generation of disease.Weeds, birds and insect etc.After crop harvesting, conventionally can be with some mould, in dry process, mould can be damaged, thus be difficult for discovering, yet many mould lakes can survive, and start germination and growth in the process of lay up period, Fodder making.
Mycotoxin is produced by 4 kinds of mould Pseudomonas: Eurotium (mainly secreting aflatoxin, ochratoxin etc.), Penicillium (mainly secreting citrinin etc.).Claviceps (mainly secreting ergotoxin).Fusobacterium (mainly secreting vomitoxin, zearalenone, Fumonisin toxin etc.).Had up to now 300 kinds of mycotoxins of surpassing to be isolated and identified out, above-mentioned several toxin are now generally 8 kinds of main toxin of understanding.
Generally mould is divided into two kinds, warehousing mould and field mould by its life habit.Warehousing mould mainly refers to feed or the raw material of storage, and the mould producing under the conditions such as suitable temperature, humidity, take Eurotium as main, such mould optimum growth temperature is 25-30 ℃, and relative humidity is 80%-90%, road aspergillus exception, can infect in field, under low temperature, also can breed; Field mould refers to Penicillium, Claviceps and Fusarium (Fusarium), this type of mould Pseudomonas field bacterial strain, conventionally cereal does not just infect before gathering, optimum growth temperature is 5-25 ℃, such mould also can breed in low temperature environment, that is to say that this type of mould still can grow in the winter time, sombre wet weather is easier to these fungus growths.
Mycotoxin is the poisonous secondary metabolic product that some mould produces in growth and breeding process on cereal or feed, toxin in the production process of cereal, the manufacture of feed, storage and transportation all can produce.What livestock and poultry were caused to very large harm is the mycotoxin being produced by mould.
The method that solves mould and mycotoxin has a variety of, conventional methods to have: radiation sterilization, interpolation mould inhibitor, and break through on mouldproof packaging, material.And the main method of adding mould inhibitor has: 1, physisorphtion, as montmorillonite is processed, can adsorb mould and mycotoxin in mass efficient ground, the adsorption capacity of physical absorbent conventionally with adsorbent with the distribution of total charge dosage, electric charge, the size of hole and available surface area (specific area) are relevant, and be decided by the physical features such as polarity, solubility, molecular size, molecular shape, CHARGE DISTRIBUTION of the lps molecule that is adsorbed; Therefore there is certain defect in physical absorbent: show as that absorption affinity is limited, efficiency is low, can not guarantee that all moulds are all had to adsorption effect.2, chemical ablation method, as added propionic acid or sorbic acid salt, can destroy mould function, hinders mould metabolism, reduces the toxic and side effect of the multiple moulds such as aspergillus flavus, Aspergillus ochraceus, aspergillus fumigatus, zeranol; Different chemical substances often only effectively can not be degraded to other toxin to certain toxin, and the chemical substance of use is residual and produce new poisonous and harmful effect in feed.3, biological degradation method, mainly contains enzymatic isolation method and microbe fermentation method, but the biological detoxication method cost using is at present higher and effect is unstable, thus in production practices still difficulty apply, etc.
At present, on market, the de-mould dose of kind of feed is a lot, is mostly clay class adsorbent, and it is difference distinguishing because of clay kind and source to some extent to the adsorption capacity of mycotoxin.This type of clay class adsorbent is owing to having multiple stratiform space structure, in absorbing mycotoxin, also can adsorb nutriment, and it is adsorbing selectively stronger, different types of clay only has suction-operated to several or single mycotoxin, so in use exist some defects and drawback.
Summary of the invention
In order to solve the problem of above-mentioned existence, the present invention has prepared the feed addictive with antifungi and toxin function thereof for a kind of ox, can be effectively, the negative effect that brings because of mould and mycotoxin in quick solution and alleviation crop and feed.
The object of the invention is to antifungi toxin feed addictive for a kind of ox.
The technical solution used in the present invention is:
An antifungi toxin feed addictive for ox, its raw material by following parts by weight is made: 10～14 parts of the roots of large-flowered skullcap, 8～12 parts of cloves, 8～12 parts of peppermints, 12～18 parts, the leaf of bamboo, 1～5 part of Tea Polyphenols, 3～8 parts of sodium Diacetates, 30～50 parts of nano montmorillonites, 8～25 parts of active saccharomyces cerevisiaes.
Further, an antifungi toxin feed addictive for ox, its raw material by following weight portion array is made: 12 parts of the roots of large-flowered skullcap, 10 parts of cloves, 10 parts of peppermints, 15 parts, the leaf of bamboo, 1.5 parts of Tea Polyphenols, 5.5 parts of sodium Diacetates, 45 parts of nano montmorillonites, 20 parts of active saccharomyces cerevisiaes.
Further, the viable bacteria concentration of above-mentioned active saccharomyces cerevisiae is no less than 2 * 10
10individual/gram.
The invention has the beneficial effects as follows:
1) additive of the present invention be take Chinese herbal and crude drugs preparations as main, is aided with the necessary salinity of animal body and useful active saccharomyces cerevisiae preparation, uses for a long time drug residue free and toxic and side effect, meets the requirement of national green food production.Especially can effectively absorb and remove the harm that mould and mycotoxin bring to animal, improve beef cattle feed intake, promote growth of animal, improve animal immune ability, reduce disease and occur, especially animal alimentary canal diseases be had to obvious prevention and control action.
2) additive of the present invention effectively absorbing mycotoxin, prevent ox feed mold, effectively promote body to resist the absorption to mycotoxin.
3) additive raw material of the present invention can obtain in a large number, suitable mass industrialized production.
The specific embodiment
The action principle of antifungi toxin feed addictive for ox of the present invention:
Although prescription component of the present invention is few, but material resources absorption principle, chemical ablation principle, biodegradation principle have been forgiven, and traditional Chinese medical science conditioning and Principles, make these four kinds to act on the effect that plays collaborative promotion in releasing mould and mycotoxin mechanism, make mould and mycotoxin be controlled effectively and remove to links in body from external, and promote the reparation of animal and health-care effect.Montmorillonite described in the present invention's formula, has another name called montmorillonite, is a kind of alumino-silicate, has very strong suction-operated, and especially nano montmorillonite, is the first-selection of mould adsorbent, and is a kind of safe green product; A kind of edible chemicals of sodium Diacetate, the fungusproof anti-corrosion agent of feed and food, has the effect that increases feed intake and weightening finish; Tea Polyphenols has very strong antioxidation, can be used for food fresh keeping anticorrosive mildewproof, have no side effect, and edible safety; Active saccharomyces cerevisiae can increase profitable strain quantity in livestock and poultry body, reduces harmful mould living space, by its toxicity of enzyme preparation degrades; Baikal skullcap root has heat-clearing and damp-drying drug, purging intense heat and detonicating, and hemostasis, antiabortive, hypotensive, for damp-warm syndrome, the heat temperature evil of vomitting uncomfortable in chest, damp and hot ruffian is full, rushes down dysentery, jaundice etc.; Cloves has in temperature, warms up kidney, falls contrary effect, and hiccup, vomiting, gastric disorder causing nausea, dysentery, ringworm etc. are all had to curative effect; Peppermint has dispelling wind, heat radiation, ward off dirty, detoxicating functions; The leaf of bamboo is very common in south, has effect clearing heat and detoxicating, anti-inflammation.The described root of large-flowered skullcap, cloves, peppermint, the leaf of bamboo, Tea Polyphenols, sodium Diacetate, nano montmorillonite, active saccharomyces cerevisiae meet the relevant regulations in < < Chinese veterinary pharmacopoeia > >.The Bile duct injury that above-mentioned Chinese traditional medicine composition can cause mycotoxin, liver renal toxicity play protection and physiotherapy function, can protect the liver and protect courage, nest is protected in enriching yin, promote immunity, repair the impaired internal organs of animal, reply organ physiological function.
Below in conjunction with specific embodiment, the present invention is further illustrated, but be not limited to this.
embodiment 1:
A beef cattle anti-heat stress feed additive, takes 12 parts of the roots of large-flowered skullcap, 10 parts of cloves, 10 parts of peppermints, 15 parts, the leaf of bamboo, 1.5 parts of Tea Polyphenols, 5.5 parts of sodium Diacetates, 35 parts of nano montmorillonites, 10 parts of active saccharomyces cerevisiaes by weight.The root of large-flowered skullcap, cloves, peppermint, the leaf of bamboo are mixed and carry out ultramicro grinding, cross 60 order shaking screens, then stir with Tea Polyphenols, sodium Diacetate, nano montmorillonite, 60 ℃ of oven dry, sterilizing, cooling, then mix packing with active saccharomyces cerevisiae.
embodiment 2:
A beef cattle anti-heat stress feed additive, takes 12 parts of the roots of large-flowered skullcap, 10 parts of cloves, 10 parts of peppermints, 15 parts, the leaf of bamboo, 1.5 parts of Tea Polyphenols, 5.5 parts of sodium Diacetates, 45 parts of nano montmorillonites, 20 parts of active saccharomyces cerevisiaes by weight.The root of large-flowered skullcap, cloves, peppermint, the leaf of bamboo are mixed and carry out ultramicro grinding, cross 60 order shaking screens, then stir with Tea Polyphenols, sodium Diacetate, nano montmorillonite, 60 ℃ of oven dry, sterilizing, cooling, then mix packing with active saccharomyces cerevisiae.
embodiment 3:
A beef cattle anti-heat stress feed additive, takes 10 parts of the roots of large-flowered skullcap, 8 parts of cloves, 12 parts of peppermints, 18 parts, the leaf of bamboo, 5 parts of Tea Polyphenols, 8 parts of sodium Diacetates, 30 parts of nano montmorillonites, 25 parts of active saccharomyces cerevisiaes by weight.The root of large-flowered skullcap, cloves, peppermint, the leaf of bamboo are mixed and carry out ultramicro grinding, cross 60 order shaking screens, then stir with Tea Polyphenols, sodium Diacetate, nano montmorillonite, 60 ℃ of oven dry, sterilizing, cooling, then mix packing with active saccharomyces cerevisiae.
embodiment 4:
A beef cattle anti-heat stress feed additive, takes 14 parts of the roots of large-flowered skullcap, 12 parts of cloves, 8 parts of peppermints, 12 parts, the leaf of bamboo, 1 part of Tea Polyphenols, 3 parts of sodium Diacetates, 50 parts of nano montmorillonites, 8 parts of active saccharomyces cerevisiaes by weight.The root of large-flowered skullcap, cloves, peppermint, the leaf of bamboo are mixed and carry out ultramicro grinding, cross 60 order shaking screens, then stir with Tea Polyphenols, sodium Diacetate, nano montmorillonite, 60 ℃ of oven dry, sterilizing, cooling, then mix packing with active saccharomyces cerevisiae.
To beef cattle prepared in embodiment, with anti-heat stress feed additive, can make further effect detection below.
one, the detoxification efficiency of antifungi toxin feed addictive of the present invention to multiple mycotoxin
In laboratory, carry out the detoxification efficiency test of the embodiment of the present invention 2 antifungi toxin feed addictives to mycotoxin.Preparing respectively zearalenone (2 μ g/mL), vomitoxin (5 μ g/mL), AFB1 (AFB1) (0.1 μ g/mL), FT is (0.05 μ g/mL), fumonisin B1(1 μ g/mL) standard liquid, get centrifuge tube and add 10 mg antifungi toxin of the present invention feed addictive, add the above mycotoxin standard liquid of 10ml, making the concentration of antifungi toxin feed addictive in solution is 2 groups of 2.5mg/ml(embodiment again).Blank group (not adding detoxifying agent) and comparative example group (adding modified montmorillonite used) are set simultaneously, and all samples is established 3 repetitions.All samples adsorbs 2h with vibration in 37 ℃ of water bath with thermostatic control oscillators.Then, with centrifugal collection supernatant, measure the content of each mycotoxin in supernatant and calculate adsorption rate Y (%)=100 * (C of each mycotoxin
0-C
1)/C
0, C
0concentration for each mycotoxin before adsorbing; C
1concentration for each mycotoxin after adsorbing.
The PBS cushioning liquid that above-mentioned precipitation after centrifugal is added to 10ml pH 7.0, the desorb 30min that vibrates in the constant temperature oscillation water-bath of 37 ℃, centrifugal collection supernatant, measures the content of each mycotoxin in supernatant, and calculates desorption efficiency R (%)=C
2/ (C
0-C
1) * 100%, C
2concentration for each mycotoxin after desorb.
Testing result is as shown in table 1, antifungi toxin feed addictive of the present invention is that (0.05 μ g/mL), fumonisin B1 adsorption rate are respectively 93.25%, 95.84%, 97.60%, 97.29% and 96.75% to zearalenone (2 μ g/mL), vomitoxin (5 μ g/mL), AFB1 (AFB1) (0.1 μ g/mL), FT, illustrate that antifungi toxin feed addictive of the present invention can effectively adsorb above-mentioned mycotoxin and be combined with toxin closely, detoxification is more thorough, and it is modified montmorillonite used that suction-operated is obviously better than commonly using.
The detoxification result of the test of table 1 embodiment of the present invention 2 antifungi toxin feed addictives to each mycotoxin
two, the impact of antifungi toxin feed addictive on beef cattle production
Consumption and usage: the feed addictive of the embodiment of the present invention 2 preparation is evenly sneaked into and in fine fodder, carried out free choice feeding according to percentage by weight 5 ‰.
Animal used as test and experimental design: from Xinfeng, Shaoguan De Feng Farming Ltd., choose 60 of the hybridization finished cattles introduced from Jiangxi, be divided at random 4 groups, 15 every group.
Experimental period: 10 ~ July 10 May
Feeding time and mode: every day twice, morning 8:00 and afternoon 5:00, by above-mentioned 4 groups of fertile oxen blue or green herbage material of first feeding, then embodiment 1 and 2 groups are fed respectively to the additive of embodiment 1,2 preparations and the compound of concentrated feed; The blank group concentrated feed of only feeding; The feed compound of the modified montmorillonite used and concentrated feed of the interpolation of equivalent weight of comparative example group.Fine fodder mainly feed corn flour, wheat bran, dregs of beans etc., be aided with a small amount of sodium acid carbonate, salt.
Measurement parameter: feed intake, weightening finish;
Measuring method: feed intake, records the concentrated feed mean value of every consumption in 1-20,21-40,41-60 day.Weighing measurement, the 1st, 21,41,61 day morning 7 empty stomaches weigh, calculate daily gain (in Table 2).
Other: morbidity and health condition.
The impact of table 2 antifungi toxin feed addictive on beef cattle production
From showing, can find out, embodiment 1 and embodiment group are all better than control group and comparative example group in feed intake and daily gain, embodiment 2 best results in all prescriptions wherein, and daily gain improves approximately 55% than control group, is utmost point significant difference (P ﹤ 0.01).
In addition, control group has 3 ox performances symptom of diarrhea in various degree; In comparative example, have the more serious symptom of diarrhea of a head of cattle performance, 1 group of embodiment has 1 ox slightly to suffer from diarrhoea, and 2 groups of embodiment all show in good health.
The explanation of above-mentioned experimental result feed feed addictive of the present invention effectively the present invention effectively absorbing mycotoxin, prevent the harm that ox feed mold, absorption and releasing mould and mycotoxin bring to animal, improve beef cattle feed intake, promote growth of animal, improve animal immune ability, reduce disease and occur, especially animal alimentary canal diseases is had to obvious prevention and control action.
Claims (3)
1. an antifungi toxin feed addictive for ox, is characterized in that: its raw material by following parts by weight is made: 10～14 parts of the roots of large-flowered skullcap, 8～12 parts of cloves, 8～12 parts of peppermints, 12～18 parts, the leaf of bamboo, 1～5 part of Tea Polyphenols, 3～8 parts of sodium Diacetates, 30～50 parts of nano montmorillonites, 8～25 parts of active saccharomyces cerevisiaes.
2. antifungi toxin feed addictive for a kind of ox according to claim 1, is characterized in that: its raw material by following weight portion array is made: 12 parts of the roots of large-flowered skullcap, 10 parts of cloves, 10 parts of peppermints, 15 parts, the leaf of bamboo, 1.5 parts of Tea Polyphenols, 5.5 parts of sodium Diacetates, 45 parts of nano montmorillonites, 20 parts of active saccharomyces cerevisiaes.
3. antifungi toxin feed addictive for a kind of ox according to claim 1 and 2, is characterized in that: the viable bacteria concentration of described active saccharomyces cerevisiae is no less than 2 * 10
10individual/gram.
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| CN111298776A (en) * | 2020-03-03 | 2020-06-19 | 武汉轻工大学 | Magnetic chitin carbonized microsphere adsorbent and preparation method and application thereof |
| CN112617017A (en) * | 2020-12-22 | 2021-04-09 | 宜兴市天石饲料有限公司 | Natural plant extraction type mildew preventive for preventing and treating feed mildew after long-term storage |
| CN112841433A (en) * | 2020-12-31 | 2021-05-28 | 江西正邦动物保健品有限公司 | Feeding composition for improving immunity of swinery and purifying vivotoxin and preparation method thereof |
| CN114052130A (en) * | 2021-12-02 | 2022-02-18 | 吉林大学 | Composition for promoting growth of yaks and preparation method thereof |
| CN115428885A (en) * | 2021-06-02 | 2022-12-06 | 菲吉乐科(南京)生物科技有限公司 | Broad-spectrum mildew removing agent capable of simultaneously adsorbing mycotoxin, endotoxin and enterotoxin |
| CN115957310A (en) * | 2022-12-28 | 2023-04-14 | 山东宝来利来生物工程股份有限公司 | Mycotoxin antidote as well as preparation method and application thereof |
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