CN108740336A - A kind of method that composite Chinese herbal extract prepares antibiotic substitute - Google Patents
A kind of method that composite Chinese herbal extract prepares antibiotic substitute Download PDFInfo
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- CN108740336A CN108740336A CN201810610420.XA CN201810610420A CN108740336A CN 108740336 A CN108740336 A CN 108740336A CN 201810610420 A CN201810610420 A CN 201810610420A CN 108740336 A CN108740336 A CN 108740336A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a kind of methods that composite Chinese herbal extract prepares antibiotic substitute, include the following steps：After several Chinese herbal medicines such as Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhizae are mixed in a certain ratio crushing, it is spare to cross 60 mesh sieve；Middle medicinal herbs after crushing are added in fermentation tank, add nutriment and water as culture medium；Access bacillus subtilis is fermented after medium sterilization cooling in fermentation tank；Zymotic fluid obtains concentrate through supernatant, supernatant is obtained by filtration after fermentation after being concentrated in vacuo again, and concentrate is adsorbed with adsorbent, and adsorbent can be prepared into feeding antibiotic substitute finished product after drying and crushing.Feeding antibiotic substitute prepared by this method has good growth promotion and disease-resistant performance, can substitute feeding antibiotic completely in feedstuff industry, there is good application prospect.
Description
Technical field
The present invention relates to a kind of methods that composite Chinese herbal extract prepares antibiotic substitute, belong to feed addictive system
Standby technical field.
Technical background
Antibiotic drug had once been saved countless life with the power of its eliminating bacteria, will felt from being born so far
The patient for catching light or heavy various diseases saves from pain to be come out.However excessively it can lead to germ drug resistance using antibiotic
Some row problems such as enhancing.Currently, antibiotic overuse is a global medicine, society and public health problem.It is anti-
Raw element is applied to the history that feedstuff industry has 50 years or so, and the development for animal husbandry has played great impetus, in order to pre-
It anti-disease and increases economic efficiency, widespread practice is intended to add antibiotic and chemical synthesis class drug in feed.Generation according to statistics
There are 20 Multiple Classes of Antibiotics and ten several synthetic antimicrobial class drugs to be applied in feed in boundary.But with people's level of understanding
It is continuously improved, it has been found that under cover unconquerable drawback behind the great economic benefit caused by antibiotic.It is such as thin
The enhancing of bacterium drug resistance, antibiotic rely on, autogenous infection caused by antibiotic, suprainfection, animal immunizing power decline and
The problems such as antibiotic residue in poultry and aquatic products product and environment, all brings feed industry, aquaculture and human health negative
Face is rung.In view of the adverse effect of antibiotic, researcher, which has been devoted to develop some, can enhance animal immunizing power, improve
Economic benefit protects the Substitutes For Antibiotic of the mankind and Environmental security again.
Chinese herbal medicine is the rarity in Chinese medicine treasure-house, and Chinese herbal medicine has been opened as feed addictive in Ancient Times in China
Begin.Chinese herbal medicine, which has, to have no toxic side effect, is not easy to cause the characteristics such as resistance, noresidue, as a kind of feeding antibiotic
Green substitute has been widely recognized in feed industry.From the 1980s, Chinese herbal medicine has been used for making feeding anti-
Raw element substitute, and achieve certain application effect.After Chinese herbal medicine undergoes microbial fermentation, active ingredient is fully released
It puts, it is even more research hotspot so that it is had more bioactivity and curative effect in recent years.What fermentation of Chinese herbal medicine had maximum at present is to make
It is bigger than normal with dosage.This not only adds the use costs of product, and have an effect on feed nutrition proportioning and palatability, so that
Fermentation of Chinese herbal medicine is widely used still without in feed industry.Therefore, it is badly in need of increasing substantially in fermentation of Chinese herbal medicine effectively
The antibacterial activity of ingredient, reduce fermentation of Chinese herbal medicine feed industry dosage, to promote fermentation of Chinese herbal medicine in feed industry
Extensive use.
Invention content
A kind of a kind of side of preparation the purpose of the present invention is to provide composite Chinese herbal extract as antibiotic substitute
Method.
The technical solution adopted by the present invention is as follows：
Prepare herb powder：Weigh 15-25 parts of Rhizoma Atractylodis Macrocephalae, 10-20 parts of pueraria lobata, 10-20 parts of the coptis, 15-25 parts of Poria cocos, Radix Glycyrrhizae 25-
35 parts, co-grinding obtains herb powder, crosses 60 mesh sieve, and sterilizing is spare.
The herb powder obtained in previous step is added in fermentation tank, and addition water and nutrients are trained into fermentation tank
Support base.Chinese herbal medicine, water, nutrients weight ratio be 1：10：0.1.Nutrients by glucose, yeast extract, sodium chloride, magnesium sulfate and
Dipotassium hydrogen phosphate forms, weight ratio 1：0.15-0.3：0.03-0.09：0.003-0.015：0.06-0.24.
The culture obtained in previous step is based on 121 DEG C of sterilizing 20min.
Culture medium after sterilizing is cooled to room temperature, bacillus subtilis seed liquor is then accessed.Culture and withered grass bud
Spore bacillus seed liquor weight ratio is 20：1.
Fermentation processes：30 DEG C -32 DEG C of fermentation temperature, filtrated air intake are 0.5vvm-2.5vvm, and fermentation tank turns
Speed is 150rpm-450rpm, and fermentation time 35-45h, ph value is 7.0-7.2.
Product handling system：After fermentation, zymotic fluid collects supernatant, supernatant is through being concentrated in vacuo by filtering to remove precipitation
It is then 1-3 in mass ratio by concentrate and adsorbent to the 1/3-1/5 of original volume:1 ratio carries out mixing and absorption, absorption
System can be prepared into feeding antibiotic substitute finished product after drying and crushing.
Further, the Chinese herbal medicine weight ratio is 1：0.6：0.6：1：1.5.
Further, the weight ratio of the nutrients is 1：0.24：0.06：0.009：0.15.
Advantages of the present invention：
Chinese herbal medicine extract provided by the invention can be added with substitute antibiotics in feed, can significantly improve the growth of livestock
Performance.
Specific implementation mode
For a better understanding of the present invention, the selection below with regard to the type of Chinese herbal medicine, fermentation parameter and fermentation method combines
The invention will be further described for embodiment, and but the scope of the present invention is not limited thereto.
Embodiment 1:Preparation method
Weigh 20 parts of Rhizoma Atractylodis Macrocephalae, 12 parts of pueraria lobata, 12 parts of the coptis, 20 parts of Poria cocos, 30 parts of Radix Glycyrrhizae, co-grinding obtains herb powder, mistake
60 mesh sieve, and sterilizing is spare.
Herb powder, 700 grams of glucose, 120 grams of yeast extracts, 50 grams of sodium chloride, 10 grams of magnesium sulfate, phosphorus are mixed by 10 kilograms
100 grams of sour hydrogen dipotassium and 100 kg of water mixing together, obtain the fermentation medium after mixing；Culture is based on 121 DEG C in fermentation tank
It is spare after sterilizing 20min.
5 kilograms of bacillus subtilis seed liquors are accessed in the fermentation tank cooling to sterilizing.
Fermentation temperature is 32 DEG C, and filtrated air intake is 2.0vvm, and the rotating speed of fermentation tank is 400rpm, and fermentation time is
35h。
After fermentation, filtering fermentation liquor removes precipitation, collects supernatant, supernatant is through being concentrated by evaporation to original volume
1/3。
Concentrate is by powdered rice hulls in mass ratio 1.5:1 ratio absorption, adsorbent can be prepared into after drying and crushing
A kind of Chinese herbal medicine antibiotic substitute finished product.
Embodiment 2：Effect example
One, materials and methods
1, material：The antibiotic substitute product prepared according to 1 method of embodiment
2, method
2.1 experimental animals and grouping
The 10 nest weanling pigs that age in days is 28 ages in days are chosen, are divided into 2 groups, every group of 5 repetitions, control group fed basal diet tries
Test the Chinese herbal medicine extract that group adds the preparation of 600mg/kg embodiments 1 on the basis of basal diet.
2.2 method for breeding
10 days test periods.It is protected using conventinal breeding and immune, free choice feeding, free water, each group rearing conditions in experimental period
It holds consistent.
2.3 test index
Growth performance：The first starting weight of statistics, latter stage weight, daily gain, feedstuff-meat ratio.
Two, result
It the results are shown in Table 1.
Influence of 1 Chinese herbal medicine extract of table to Growth Performance of Weaning Piglets
As shown in Table 1, the daily gain of test group is higher than control group, and feedstuff-meat ratio is less than control group.
The above results show that Chinese herbal medicine extract provided by the invention is added in feed and are remarkably improved weanling pig
Growth performance, it was demonstrated that the Chinese herbal medicine extract of the present invention plays the role of similar antibiotic, can substitute to a certain extent anti-
The use of raw element.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but the not primary protection for limiting the present invention
Range.It will be understood by those of ordinary skill in the art that technical scheme of the present invention can be modified or replaced equivalently,
Without departing from the essence and protection domain of technical solution of the present invention.
Claims (8)
1. a kind of preparation method of composite Chinese herbal extract, which is characterized in that be made by the steps：
（1）Chinese herbal medicine used, co-grinding are weighed by a certain percentage；
（2）60 mesh sieve is smashed it through, sterilizing is spare；
（3）By step（2）Chinese herbal medicine after crushing is added in fermentation tank, and water and nutrients, mixing are added into fermentation tank
After obtain fermentation medium；
（4）By step（3）Culture in the fermentation tank of acquisition is based on 121 DEG C of sterilizing 20min；
（5）Culture medium in fermentation tank after sterilizing is cooled to room temperature, fermentation of bacillus subtilis culture 24-48 is then accessed
Hour, control ph 7.0-7.2, fermentation temperature is 28 DEG C -30 DEG C；
（6）Product handling system：After fermentation, filtering fermentation liquor removes precipitation, collects supernatant；Supernatant is concentrated in vacuo to substance
Long-pending 1/3-1/5；Then it is 1-3 in mass ratio by concentrate and adsorbent:1 ratio carries out mixing and absorption, adsorbent drying
With after crushing i.e. can be made into feeding antibiotic substitute finished product.
2. a kind of preparation method of composite Chinese herbal extract according to claim 1, it is characterised in that：The step
（1）Used in Chinese herbal medicine be 15-25 parts of Rhizoma Atractylodis Macrocephalae, 10-20 parts of pueraria lobata, 10-20 parts of the coptis, 15-25 parts of Poria cocos, Radix Glycyrrhizae 25-35
Part.
3. a kind of preparation method of composite Chinese herbal extract according to claim 2, it is characterised in that：The Chinese herbal medicine
Weight ratio is 1：0.6：0.6：1：1.5.
4. a kind of preparation method of composite Chinese herbal extract according to claim 1, it is characterised in that：The step
（3）In nutrients be made of glucose, yeast extract, sodium chloride, magnesium sulfate and dipotassium hydrogen phosphate, weight ratio 1：0.15-
0.3：0.03-0.09：0.003-0.015：0.06-0.24.
5. a kind of preparation method of composite Chinese herbal extract according to claim 4, it is characterised in that：The nutrients
Weight ratio be 1：0.24：0.06：0.009：0.15.
6. a kind of preparation method of composite Chinese herbal extract according to claim 1, it is characterised in that：The step
（3）In Chinese herbal medicine, water, nutrients weight ratio be 1：10：0.1.
7. a kind of preparation method of composite Chinese herbal extract according to claim 1, it is characterised in that：The step
（5）Middle fermentation culture medium and bacillus subtilis seed liquor weight ratio are 20：1.
8. a kind of preparation method of composite Chinese herbal extract according to claim 1, it is characterised in that：The step
（6）Middle adsorbent is one kind in corn flour, powdered rice hulls, oyster shell whiting, zeolite powder.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112715747A (en) * | 2020-10-20 | 2021-04-30 | 合肥中科亮视医学科技有限公司 | Intelligent screening method of Chinese herbal medicine compound for replacing feed antibiotics |
| CN115334898A (en) * | 2020-05-21 | 2022-11-11 | 汉坰妊 | Environment-friendly growth auxiliary and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115334898A (en) * | 2020-05-21 | 2022-11-11 | 汉坰妊 | Environment-friendly growth auxiliary and preparation method thereof |
| CN112715747A (en) * | 2020-10-20 | 2021-04-30 | 合肥中科亮视医学科技有限公司 | Intelligent screening method of Chinese herbal medicine compound for replacing feed antibiotics |
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Application publication date: 20181106 |