CN104489408A - Porcine feed additive for effectively improving intestinal health and enhancing immunity - Google Patents
Porcine feed additive for effectively improving intestinal health and enhancing immunity Download PDFInfo
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Abstract
The invention provides a porcine feed additive for effectively improving intestinal health and enhancing immunity. The additive consists of multiple Chinese herbal medicines, plant extracts, an acidifier, a microbial preparation, amino acids, mineral elements and the like. The additive is scientific in compatibility and can be used for effectively enhancing the porcine immunity, reducing the porcine morbidity and replacing or partially replacing use of antibiotics and the like in feeds. In addition, the additive can be further used for improving intestinal health and promoting digestive absorption of the feed by the pig, and meanwhile, a breeding house is cleaned, and the environment of the breeding house is protected and improved. The additive helps promot healthy and order development of feed and livestock industry and has important meaning for improving the breeding benefit and lowering the breeding risk.
Description
Technical field
The present invention relates to a kind of pig's feed additive, especially relate to a kind of effective pig's feed additive improving intestinal health and improve immunity, belong to feed additive field.
Background technology
In recent years, the food-safety problem caused because antibiotics feed addictive abuse appears in the newspapers again and again, and cause thus directly and indirect economic loss number is huge makes people startling, the environmental problem brought thus in addition also should not be underestimated.Antibiotic has can not by the characteristic of the complete metabolism of humans and animals body, form greatly mainly with original shape and active metabolite is discharged to external by ight soil, through a series of biotransformations such as absorption, photodissociation, hydrolysis and microbial degradations, the direct influence ecological environment of meeting, remote-effects health.Swine disease cultivates maximum risk simultaneously, and reducing the pig incidence of disease is a great problem being difficult in aquaculture break through, and seriously governs the development of pig industry.
The enteron aisle of pig is the main place that food digestion absorbs, and has important effect to the healthy growth maintaining pig.At enteron aisle, the various nutriments in chyme are broken down into various small-molecule substance, absorb and enter blood and lymph, utilize for body each several part through enteron aisle fine hair.In addition, in body defenses process, enteron aisle also plays an important role.Improve pig intestinal health, be conducive to improving feed digestibility, improve the use value of feed, the immunity of pig can also be strengthened, to the active influence that grown of pig simultaneously.
Therefore; develop the little additive of a kind of natural, nutrition, toxic and side effect to improve the immunity of pig and to improve intestinal health and seem particularly important; antibiotic use can not only be reduced; protection human health; the pig incidence of disease can also be reduced, reduce cultivation risk, be conducive to promoting feed and the healthy and orderly development of animal husbandry; for raising culture benefit, reduce cultivation risk significant.
Summary of the invention
The object of the present invention is to provide a kind of effective pig's feed additive improving intestinal health and improve immunity.This additive can strengthen the immunity of pig, and reduce the incidence of disease of pig, be conducive to reducing antibiotic use, this additive can also improve intestinal health simultaneously, promotes pig growth, shortens marketing time, promote culture efficiency, increase economic benefit.
Technical scheme provided by the invention is as follows:
A kind of effective pig's feed additive improving intestinal health and improve immunity, every 100 parts are made up of following raw materials by weight portion: Pogostemon cablin powder 5-8 part, India bloom 5-8 part, Asiatic plantain powder 3-5 part, refining burdock root powder 2-3 part, Caulis Lonicerae extract 2-3 part, fennel seeds powder 2-4 part, stachyurus pith powder 2-4 part, horsewhip grass meal 1-2 part, dark plum powder 1-2 part, radix polygonati officinalis powder 1-2 part, Radix zanthoxyli powder 1-2 part, acidulant 2-3 part, probiotics 2-3 part, L-threonine 0.2-0.3 part, L-Trp 0.1-0.2 part, L-arginine 0.3-0.5 part, glutamic acid 0.1-0.2 part, vitamin A 0.05-0.08 part, Cobastab
10.03-0.05 part, Cobastab
20.03-0.05 part, vitamin D
30.01-0.03 part, D-VB5 calcium 0.03-0.05 part, ferrous fumarate 0.3-0.5 part, zinc sulfate 0.3-0.5 part, KI 0.05-0.1 part, cobalt chloride 0.01-0.03 part, sodium molybdate 0.01-0.03 part, copper methionine 0.3-0.6 part, mannitol 0.02-0.04 part, potassium alginate 0.03-0.05 part, chitosan oligomer 0.01-0.03 part, all the other are carrier, carrier is defatted rice bran and wheat bran 1:1-3 composition in mass ratio, and defatted rice bran and wheat bran pulverized 40 mesh sieves in advance.
The preparation method of described refining burdock root powder is:
(1) fresh radix bardanae is removed the peel, chopping, be placed in air dry oven and dry, make its moisture lower than 12%, pulverize, cross 60 mesh sieves, obtain burdock root powder;
(2) by burdock root powder and complex enzyme formulation, sodium chloride, magnesium chloride 100:(0.5-1 by weight): (0.5-0.8): (0.5-1) mixes, afterwards again by solid-liquid ratio 1:(15-20) add the ethanol of 50-60%, soak 3-5h, soaking temperature is 40-45 DEG C, filter, get filter residue; Described complex enzyme formulation is by cellulase, hemicellulase and pectase 3:(1-2 by weight): (1-3) mixes;
(3) filter residue step (2) obtained is dried, then by solid-liquid ratio 1:(10-20) adding distilled water, refluxing extraction 2-3h, Extracting temperature is 35-40 DEG C, filters, gets filtrate, repeat extraction 2 times, merging filtrate;
(4) by filtrate concentrate drying, pulverized 60 mesh sieves, obtained refining burdock root powder, preserved at-20 DEG C of temperature lower seals;
The preparation method of described Caulis Lonicerae extract is:
(1) caulis lonicerae of drying is pulverized, cross 60 mesh sieves, with hemicellulase and pectase 100:(0.3-0.5 by weight): (0.5-1) mixes, then by solid-liquid ratio 1:(5-10) add distilled water, soak 3-5h, soaking temperature is 5-10 DEG C, soak complete filtration afterwards, get filter residue, dry, make its moisture lower than 15%, bake out temperature is 60-65 DEG C;
(2) by filter residue and the aluminum sulfate after drying and crab shell powder by weight 50:(1-3): (1-2) mix, by solid-liquid ratio 1:(5-8) add distilled water, immersion 2-4h, soaking temperature is 5-10 DEG C, and filtration, gets filter residue;
(3) filter residue is dried, by solid-liquid ratio 1:(10-15) add the ethanol of 60-70%, refluxing extraction 2-3h, Extracting temperature is 40-50 DEG C, gets filtrate, and filter residue extracts 1 time again, merges the filtrate of 2 extractions;
(4) filtrate is concentrated, spraying dry, pulverized 60 mesh sieves, and obtained Caulis Lonicerae extract;
The preparation method of described Pogostemon cablin powder is: Pogostemon cablin was pulverized 60 mesh sieves and namely obtain Pogostemon cablin powder;
The preparation method of described India bloom is: Tabasheer was pulverized 60 mesh sieves and namely obtain India bloom;
The preparation method of described Asiatic plantain powder is: Asiatic plantain was pulverized 60 mesh sieves and namely obtain Asiatic plantain powder;
The preparation method of described fennel seeds powder is: fennel seeds were pulverized 60 mesh sieves and namely obtain fennel seeds powder;
The preparation method of described stachyurus pith powder is: stachyurus pith was pulverized 60 mesh sieves and namely obtain stachyurus pith powder;
The preparation method of described horsewhip grass meal is: Verbena officinalis was pulverized 60 mesh sieves and namely obtain horsewhip grass meal;
The preparation method of described dark plum powder is: dark plum was pulverized 60 mesh sieves and namely obtain dark plum powder;
The preparation method of described radix polygonati officinalis powder is: radix polygonati officinalis was pulverized 60 mesh sieves and namely obtain radix polygonati officinalis powder;
The preparation method of described Radix zanthoxyli powder is: Radix zanthoxyli was pulverized 60 mesh sieves and namely obtain Radix zanthoxyli powder;
Described acidulant is by malic acid, potassium citrate and tartaric acid 1:(1-3 by weight): (2-3) forms;
Described probiotics by bacillus pumilus, Bifidobacterium, lactobacillus reuteri and Lactobacillus plantarum by bacteria suspension volume ratio 1:(1-2): (1-2): (2-3) mixes; Wherein, bacillus pumilus bacteria suspension concentration is 1.8 × 10
7individual/ml, Bifidobacterium bacteria suspension concentration is 2.2 × 10
8individual/ml, lactobacillus reuteri bacteria suspension concentration is 2.8 × 10
6individual/ml, Lactobacillus plantarum bacteria suspension concentration is 3.2 × 10
7individual/ml;
The effective pig's feed additive improving intestinal health and improve immunity, the addition in pig feed is 200-400g/t.
Beneficial effect
A kind of effective pig's feed additive improving intestinal health and raising immunity provided by the invention is made up of multiple Chinese herbal medicine, Caulis Lonicerae extract, acidulant, probiotics, amino acid and mineral matter etc., compatibility science, has the effect improving pig intestinal health and improve immunity.Radix bardanae and caulis lonicerae, after ad hoc approach process, improve concentration and the activity of the active ingredient of radix bardanae and caulis lonicerae, further enhancing effect of the present invention.Through facts have proved, the various Chinese herbal medicine side effects that the present invention uses are little, have no drug resistance, and noresidue in pork product, without carcinogenic, teratogenesis, mutagenesis, have the double action of nutrition and medicine concurrently.
Specific embodiment
The present invention is further described below by way of specific embodiment.
Embodiment 1
The effective pig's feed additive improving intestinal health and improve immunity, every 100 parts are made up of following raw materials by weight portion: 5 parts, Pogostemon cablin powder, India bloom 5 parts, 3 parts, Asiatic plantain powder, refining burdock root powder 2 parts, Caulis Lonicerae extract 2 parts, 2 parts, fennel seeds powder, 2 parts, stachyurus pith powder, horsewhip grass meal 1 part, 1 part, dark plum powder, 1 part, radix polygonati officinalis powder, 1 part, Radix zanthoxyli powder, acidulant 2 parts, probiotics 2 parts, L-threonine 0.2 part, L-Trp 0.1 part, L-arginine 0.3 part, 0.1 part, glutamic acid, vitamin A 0.05 part, Cobastab
10.03 part, Cobastab
20.03 part, vitamin D
30.01 part, D-VB5 calcium 0.03 part, ferrous fumarate 0.3 part, 0.3 part, zinc sulfate, KI 0.05 part, cobalt chloride 0.01 part, sodium molybdate 0.01 part, copper methionine 0.3 part, mannitol 0.02 part, potassium alginate 0.03 part, chitosan oligomer 0.01 part, all the other are carrier, carrier is defatted rice bran and wheat bran 1:1 composition in mass ratio, and defatted rice bran and wheat bran pulverized 40 mesh sieves in advance.
The preparation method of described refining burdock root powder is:
(1) fresh radix bardanae is removed the peel, chopping, be placed in air dry oven and dry, make its moisture lower than 12%, pulverize, cross 60 mesh sieves, obtain burdock root powder;
(2) burdock root powder and complex enzyme formulation, sodium chloride, magnesium chloride are mixed by weight 100:0.5:0.5:0.5, add the ethanol of 50% afterwards again by solid-liquid ratio 1:15, soak 3h, soaking temperature is 40 DEG C, filters, gets filter residue; Described complex enzyme formulation is mixed by weight 3:1:1 by cellulase, hemicellulase and pectase;
(3) filter residue step (2) obtained is dried, then adds distilled water by solid-liquid ratio 1:10, refluxing extraction 2h, and Extracting temperature is 35 DEG C, filters, gets filtrate, repeat extraction 2 times, merging filtrate;
(4) by filtrate concentrate drying, pulverized 60 mesh sieves, obtained refining burdock root powder, preserved at-20 DEG C of temperature lower seals;
The preparation method of described Caulis Lonicerae extract is:
(1) caulis lonicerae of drying is pulverized, cross 60 mesh sieves, mix by weight 100:0.3:0.5 with hemicellulase and pectase, then add distilled water by solid-liquid ratio 1:5, soak 3h, soaking temperature is 5 DEG C, soak complete filtration afterwards, get filter residue, dry, make its moisture lower than 15%, bake out temperature is 60 DEG C;
(2) filter residue and the aluminum sulfate after drying and crab shell powder mixs by weight 50:1:1, add distilled water by solid-liquid ratio 1:5, immersion 2h, soaking temperature is 5 DEG C, and filtration, gets filter residue;
(3) dried by filter residue, add the ethanol of 60% by solid-liquid ratio 1:10, refluxing extraction 2h, Extracting temperature is 40 DEG C, gets filtrate, and filter residue extracts 1 time again, merges the filtrate of extracting for 2 times;
(4) filtrate is concentrated, spraying dry, pulverized 60 mesh sieves, and obtained Caulis Lonicerae extract;
The preparation method of described Pogostemon cablin powder is: Pogostemon cablin was pulverized 60 mesh sieves and namely obtain Pogostemon cablin powder;
The preparation method of described India bloom is: Tabasheer was pulverized 60 mesh sieves and namely obtain India bloom;
The preparation method of described Asiatic plantain powder is: Asiatic plantain was pulverized 60 mesh sieves and namely obtain Asiatic plantain powder;
The preparation method of described fennel seeds powder is: fennel seeds were pulverized 60 mesh sieves and namely obtain fennel seeds powder;
The preparation method of described stachyurus pith powder is: stachyurus pith was pulverized 60 mesh sieves and namely obtain stachyurus pith powder;
The preparation method of described horsewhip grass meal is: Verbena officinalis was pulverized 60 mesh sieves and namely obtain horsewhip grass meal;
The preparation method of described dark plum powder is: dark plum was pulverized 60 mesh sieves and namely obtain dark plum powder;
The preparation method of described radix polygonati officinalis powder is: radix polygonati officinalis was pulverized 60 mesh sieves and namely obtain radix polygonati officinalis powder;
The preparation method of described Radix zanthoxyli powder is: Radix zanthoxyli was pulverized 60 mesh sieves and namely obtain Radix zanthoxyli powder;
Described acidulant is made up of by weight 1:1:2 malic acid, potassium citrate and tartaric acid;
Described probiotics is mixed by bacteria suspension volume ratio 1:1:1:2 by bacillus pumilus, Bifidobacterium, lactobacillus reuteri and Lactobacillus plantarum; Wherein, bacillus pumilus bacteria suspension concentration is 1.8 × 10
7individual/ml, Bifidobacterium bacteria suspension concentration is 2.2 × 10
8individual/ml, lactobacillus reuteri bacteria suspension concentration is 2.8 × 10
6individual/ml, Lactobacillus plantarum bacteria suspension concentration is 3.2 × 10
7individual/ml;
The effective pig's feed additive improving intestinal health and improve immunity, the addition in pig feed is 200g/t.
Embodiment 2
The effective pig's feed additive improving intestinal health and improve immunity, every 100 parts are made up of following raw materials by weight portion: 8 parts, Pogostemon cablin powder, India bloom 8 parts, 5 parts, Asiatic plantain powder, refining burdock root powder 3 parts, Caulis Lonicerae extract 3 parts, 4 parts, fennel seeds powder, 4 parts, stachyurus pith powder, horsewhip grass meal 2 parts, 2 parts, dark plum powder, 2 parts, radix polygonati officinalis powder, 2 parts, Radix zanthoxyli powder, acidulant 3 parts, probiotics 3 parts, L-threonine 0.3 part, L-Trp 0.2 part, L-arginine 0.5 part, 0.2 part, glutamic acid, vitamin A 0.08 part, Cobastab
10.05 part, Cobastab
20.05 part, vitamin D
30.03 part, D-VB5 calcium 0.05 part, ferrous fumarate 0.5 part, 0.5 part, zinc sulfate, KI 0.1 part, cobalt chloride 0.03 part, sodium molybdate 0.03 part, copper methionine 0.6 part, mannitol 0.04 part, potassium alginate 0.05 part, chitosan oligomer 0.03 part, all the other are carrier, carrier is defatted rice bran and wheat bran 1:3 composition in mass ratio, and defatted rice bran and wheat bran pulverized 40 mesh sieves in advance.
The preparation method of described refining burdock root powder is:
(1) fresh radix bardanae is removed the peel, chopping, be placed in air dry oven and dry, make its moisture lower than 12%, pulverize, cross 60 mesh sieves, obtain burdock root powder;
(2) burdock root powder and complex enzyme formulation, sodium chloride, magnesium chloride are mixed by weight 100:1:0.8:1, add the ethanol of 60% afterwards again by solid-liquid ratio 1:20, soak 5h, soaking temperature is 45 DEG C, filters, gets filter residue; Described complex enzyme formulation is mixed by weight 3:2:3 by cellulase, hemicellulase and pectase;
(3) filter residue step (2) obtained is dried, then adds distilled water by solid-liquid ratio 1:20, refluxing extraction 3h, and Extracting temperature is 40 DEG C, filters, gets filtrate, repeat extraction 2 times, merging filtrate;
(4) by filtrate concentrate drying, pulverized 60 mesh sieves, obtained refining burdock root powder, preserved at-20 DEG C of temperature lower seals;
The preparation method of described Caulis Lonicerae extract is:
(1) caulis lonicerae of drying is pulverized, cross 60 mesh sieves, mix by weight 100:0.5:1 with hemicellulase and pectase, then add distilled water by solid-liquid ratio 1:10, soak 5h, soaking temperature is 10 DEG C, soak complete filtration afterwards, get filter residue, dry, make its moisture lower than 15%, bake out temperature is 65 DEG C;
(2) filter residue and the aluminum sulfate after drying and crab shell powder mixs by weight 50:3:2, add distilled water by solid-liquid ratio 1:8, immersion 4h, soaking temperature is 10 DEG C, and filtration, gets filter residue;
(3) dried by filter residue, add the ethanol of 70% by solid-liquid ratio 1:15, refluxing extraction 3h, Extracting temperature is 50 DEG C, gets filtrate, and filter residue extracts 1 time again, merges the filtrate of extracting for 2 times;
(4) filtrate is concentrated, spraying dry, pulverized 60 mesh sieves, and obtained Caulis Lonicerae extract;
The preparation method of described Pogostemon cablin powder is: Pogostemon cablin was pulverized 60 mesh sieves and namely obtain Pogostemon cablin powder;
The preparation method of described India bloom is: Tabasheer was pulverized 60 mesh sieves and namely obtain India bloom;
The preparation method of described Asiatic plantain powder is: Asiatic plantain was pulverized 60 mesh sieves and namely obtain Asiatic plantain powder;
The preparation method of described fennel seeds powder is: fennel seeds were pulverized 60 mesh sieves and namely obtain fennel seeds powder;
The preparation method of described stachyurus pith powder is: stachyurus pith was pulverized 60 mesh sieves and namely obtain stachyurus pith powder;
The preparation method of described horsewhip grass meal is: Verbena officinalis was pulverized 60 mesh sieves and namely obtain horsewhip grass meal;
The preparation method of described dark plum powder is: dark plum was pulverized 60 mesh sieves and namely obtain dark plum powder;
The preparation method of described radix polygonati officinalis powder is: radix polygonati officinalis was pulverized 60 mesh sieves and namely obtain radix polygonati officinalis powder;
The preparation method of described Radix zanthoxyli powder is: Radix zanthoxyli was pulverized 60 mesh sieves and namely obtain Radix zanthoxyli powder;
Described acidulant is made up of by weight 1:3:3 malic acid, potassium citrate and tartaric acid;
Described probiotics is mixed by bacteria suspension volume ratio 1:2:2:3 by bacillus pumilus, Bifidobacterium, lactobacillus reuteri and Lactobacillus plantarum; Wherein, bacillus pumilus bacteria suspension concentration is 1.8 × 10
7individual/ml, Bifidobacterium bacteria suspension concentration is 2.2 × 10
8individual/ml, lactobacillus reuteri bacteria suspension concentration is 2.8 × 10
6individual/ml, Lactobacillus plantarum bacteria suspension concentration is 3.2 × 10
7individual/ml;
The effective pig's feed additive improving intestinal health and improve immunity, the addition in pig feed is 400g/t.
Embodiment 3
A kind of effective pig's feed additive improving intestinal health and improve immunity, every 100 parts are made up of following raw materials by weight portion: 6 parts, Pogostemon cablin powder, India bloom 6 parts, 4 parts, Asiatic plantain powder, refining burdock root powder 2.5 parts, Caulis Lonicerae extract 2.5 parts, 3 parts, fennel seeds powder, 3 parts, stachyurus pith powder, horsewhip grass meal 1.5 parts, 1.5 parts, dark plum powder, 1.5 parts, radix polygonati officinalis powder, 1.5 parts, Radix zanthoxyli powder, acidulant 2.5 parts, probiotics 2.5 parts, L-threonine 0.25 part, L-Trp 0.15 part, L-arginine 0.4 part, 0.15 part, glutamic acid, vitamin A 0.06 part, Cobastab
10.04 part, Cobastab
20.04 part, vitamin D
30.02 part, D-VB5 calcium 0.04 part, ferrous fumarate 0.4 part, 0.4 part, zinc sulfate, KI 0.08 part, cobalt chloride 0.02 part, sodium molybdate 0.02 part, copper methionine 0.4 part, mannitol 0.03 part, potassium alginate 0.04 part, chitosan oligomer 0.02 part, all the other are carrier, carrier is defatted rice bran and wheat bran 1:2 composition in mass ratio, and defatted rice bran and wheat bran pulverized 40 mesh sieves in advance.
The preparation method of described refining burdock root powder is:
(1) fresh radix bardanae is removed the peel, chopping, be placed in air dry oven and dry, make its moisture lower than 12%, pulverize, cross 60 mesh sieves, obtain burdock root powder;
(2) burdock root powder and complex enzyme formulation, sodium chloride, magnesium chloride are mixed by weight 100:0.6:0.6:0.8, add the ethanol of 55% afterwards again by solid-liquid ratio 1:18, soak 4h, soaking temperature is 42 DEG C, filters, gets filter residue; Described complex enzyme formulation is mixed by weight 3:1.5:2 by cellulase, hemicellulase and pectase;
(3) filter residue step (2) obtained is dried, then adds distilled water by solid-liquid ratio 1:15, refluxing extraction 2.5h, and Extracting temperature is 36 DEG C, filters, gets filtrate, repeat extraction 2 times, merging filtrate;
(4) by filtrate concentrate drying, pulverized 60 mesh sieves, obtained refining burdock root powder, preserved at-20 DEG C of temperature lower seals;
The preparation method of described Caulis Lonicerae extract is:
(1) caulis lonicerae of drying is pulverized, cross 60 mesh sieves, mix by weight 100:0.4:0.8 with hemicellulase and pectase, then add distilled water by solid-liquid ratio 1:8, soak 4h, soaking temperature is 8 DEG C, soak complete filtration afterwards, get filter residue, dry, make its moisture lower than 15%, bake out temperature is 62 DEG C;
(2) filter residue and the aluminum sulfate after drying and crab shell powder mixs by weight 50:2:1.5, add distilled water by solid-liquid ratio 1:6, immersion 3h, soaking temperature is 8 DEG C, and filtration, gets filter residue;
(3) dried by filter residue, add the ethanol of 65% by solid-liquid ratio 1:12, refluxing extraction 2.5h, Extracting temperature is 45 DEG C, gets filtrate, and filter residue extracts 1 time again, merges the filtrate of extracting for 2 times;
(4) filtrate is concentrated, spraying dry, pulverized 60 mesh sieves, and obtained Caulis Lonicerae extract;
The preparation method of described Pogostemon cablin powder is: Pogostemon cablin was pulverized 60 mesh sieves and namely obtain Pogostemon cablin powder;
The preparation method of described India bloom is: Tabasheer was pulverized 60 mesh sieves and namely obtain India bloom;
The preparation method of described Asiatic plantain powder is: Asiatic plantain was pulverized 60 mesh sieves and namely obtain Asiatic plantain powder;
The preparation method of described fennel seeds powder is: fennel seeds were pulverized 60 mesh sieves and namely obtain fennel seeds powder;
The preparation method of described stachyurus pith powder is: stachyurus pith was pulverized 60 mesh sieves and namely obtain stachyurus pith powder;
The preparation method of described horsewhip grass meal is: Verbena officinalis was pulverized 60 mesh sieves and namely obtain horsewhip grass meal;
The preparation method of described dark plum powder is: dark plum was pulverized 60 mesh sieves and namely obtain dark plum powder;
The preparation method of described radix polygonati officinalis powder is: radix polygonati officinalis was pulverized 60 mesh sieves and namely obtain radix polygonati officinalis powder;
The preparation method of described Radix zanthoxyli powder is: Radix zanthoxyli was pulverized 60 mesh sieves and namely obtain Radix zanthoxyli powder;
Described acidulant is made up of by weight 1:2:2.5 malic acid, potassium citrate and tartaric acid;
Described probiotics is mixed by bacteria suspension volume ratio 1:1.5:1.5:2.5 by bacillus pumilus, Bifidobacterium, lactobacillus reuteri and Lactobacillus plantarum; Wherein, bacillus pumilus bacteria suspension concentration is 1.8 × 10
7individual/ml, Bifidobacterium bacteria suspension concentration is 2.2 × 10
8individual/ml, lactobacillus reuteri bacteria suspension concentration is 2.8 × 10
6individual/ml, Lactobacillus plantarum bacteria suspension concentration is 3.2 × 10
7individual/ml;
The effective pig's feed additive improving intestinal health and improve immunity, the addition in pig feed is 300g/t.
Test example
1, experimental animal and experimental design
Test selection 200 subordinate plant of Rui Jin Twins Feed Co., Ltd. average weights are " DLY " tri-crossbreeding of about 28kg, be divided into 4 groups at random, often organize 5 repetitions, each repetition 10 pigs (male and female half and half), comprising a blank group and 3 test group.Blank group daily ration does not add any additive obtained in embodiment 1-3, all the other 3 test group swine rationses add the obtained additive of embodiment 1-3 respectively, live pig free choice feeding and drinking-water, carry out immunity and expelling parasite routinely, in experimental period, every day observes and records the mental status of pig diarrhoea, influenza and pig.Experimental period 90d.In feeding process, each group of pig incidence is added up.At the end of feeding experiment, the growth performance of pig, diarrhea rate and immune indexes are analyzed.
Data statistic analysis
Test data adopts SPSS19.0 statistical software to carry out statistical analysis, the results are shown in following table.
Table 1 growth performance index
| Project | Control group | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Daily ingestion amount/kg | 1.40 | 1.49 | 1.58 | 1.69 |
| Daily gain/kg | 0.48 | 0.53 | 0.60 | 0.74 |
| Feed-weight ratio | 2.92 | 2.81 | 2.63 | 2.28 |
Daily ingestion amount (ADFI): the feeding capacity of accurate recording each hurdle test pig and clout amount, calculates every pig average daily ingestion amount weekly and full phase average daily ingestion amount;
Daily gain (ADG): test pig starts weekly and terminates morning 7:30 and weighs on an empty stomach, calculates the average daily gain weekly of every pig and full phase average daily gain;
Feed-weight ratio: (F/G): calculate according to feed intake and weightening finish.
Table 2 immune indexes, diarrhea rate, the incidence of disease
| Project | Control group | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| IgA/(g/L) | 0.10 | 0.26 | 0.18 | 0.22 |
| IgG/(g/L) | 6.10 | 6.56 | 7.44 | 8.38 |
| IgM(g/L) | 0.99 | 1.18 | 1.26 | 1.39 |
| Diarrhea rate (%) | 14.0% | 7.3% | 6.5% | 3.3% |
| The incidence of disease (%) | 13.2% | 8.5% | 7.2% | 2.8% |
Immune indexes measures: the 7:30 in morning last day that experimental period terminates, and often group is chosen two and measured IgA, IgG and IgM level.
Diarrhea rate: diarrhea rate is experimental period diarrhoea piglet head/(test piglet head number × test number of days) × 100%.
The incidence of disease: sick pig generates heat suddenly and reaches 42 DEG C even higher, and ight soil is dry and hard, lassitude, anorexia or do not eat, normal accumbency together, is reluctant activity, expiratory dyspnea, cough, eye, nose stream mucus.
The raw material of this product is green safety material, and significantly can reduce the incidence of disease of pig, strengthen pig immunity and improve enteron aisle, can add in pig feed as antibiotic substitute, produce disease resisting effect good, strengthen pig immunity and improve the mixed feed of enteron aisle, there are good using value and wide market prospects.
Claims (6)
1. the pig's feed additive effectively improving intestinal health and improve immunity, it is characterized in that every 100 parts are made up of following raw materials by weight portion: Pogostemon cablin powder 5-8 part, India bloom 5-8 part, Asiatic plantain powder 3-5 part, refining burdock root powder 2-3 part, Caulis Lonicerae extract 2-3 part, fennel seeds powder 2-4 part, stachyurus pith powder 2-4 part, horsewhip grass meal 1-2 part, dark plum powder 1-2 part, radix polygonati officinalis powder 1-2 part, Radix zanthoxyli powder 1-2 part, acidulant 2-3 part, probiotics 2-3 part, L-threonine 0.2-0.3 part, L-Trp 0.1-0.2 part, L-arginine 0.3-0.5 part, glutamic acid 0.1-0.2 part, vitamin A 0.05-0.08 part, Cobastab
10.03-0.05 part, Cobastab
20.03-0.05 part, vitamin D
30.01-0.03 part, D-VB5 calcium 0.03-0.05 part, ferrous fumarate 0.3-0.5 part, zinc sulfate 0.3-0.5 part, KI 0.05-0.1 part, cobalt chloride 0.01-0.03 part, sodium molybdate 0.01-0.03 part, copper methionine 0.3-0.6 part, mannitol 0.02-0.04 part, potassium alginate 0.03-0.05 part, chitosan oligomer 0.01-0.03 part, all the other are carrier, carrier is defatted rice bran and wheat bran 1:1-3 composition in mass ratio, and defatted rice bran and wheat bran pulverized 40 mesh sieves in advance,
Described acidulant is by malic acid, potassium citrate and tartaric acid 1:(1-3 by weight): (2-3) forms;
Described probiotics by bacillus pumilus, Bifidobacterium, lactobacillus reuteri and Lactobacillus plantarum by bacteria suspension volume ratio 1:(1-2): (1-2): (2-3) mixes; Wherein, bacillus pumilus bacteria suspension concentration is 1.8 × 10
7individual/ml, Bifidobacterium bacteria suspension concentration is 2.2 × 10
8individual/ml, lactobacillus reuteri bacteria suspension concentration is 2.8 × 10
6individual/ml, Lactobacillus plantarum bacteria suspension concentration is 3.2 × 10
7individual/ml.
2. a kind ofly according to claim 1 effectively improve intestinal health and improve the pig's feed additive of immunity, it is characterized in that every 100 parts are made up of following raw materials by weight portion: 6 parts, Pogostemon cablin powder, India bloom 6 parts, 4 parts, Asiatic plantain powder, refining burdock root powder 2.5 parts, Caulis Lonicerae extract 2.5 parts, 3 parts, fennel seeds powder, 3 parts, stachyurus pith powder, horsewhip grass meal 1.5 parts, 1.5 parts, dark plum powder, 1.5 parts, radix polygonati officinalis powder, 1.5 parts, Radix zanthoxyli powder, acidulant 2.5 parts, probiotics 2.5 parts, L-threonine 0.25 part, L-Trp 0.15 part, L-arginine 0.4 part, 0.15 part, glutamic acid, vitamin A 0.06 part, Cobastab
10.04 part, Cobastab
20.04 part, vitamin D
30.02 part, D-VB5 calcium 0.04 part, ferrous fumarate 0.4 part, 0.4 part, zinc sulfate, KI 0.08 part, cobalt chloride 0.02 part, sodium molybdate 0.02 part, copper methionine 0.4 part, mannitol 0.03 part, potassium alginate 0.04 part, chitosan oligomer 0.02 part, all the other are carrier, carrier is defatted rice bran and wheat bran 1:2 composition in mass ratio, and defatted rice bran and wheat bran pulverized 40 mesh sieves in advance,
Described acidulant is made up of by weight 1:2:2.5 malic acid, potassium citrate and tartaric acid;
Described probiotics is mixed by bacteria suspension volume ratio 1:1.5:1.5:2.5 by bacillus pumilus, Bifidobacterium, lactobacillus reuteri and Lactobacillus plantarum; Wherein, bacillus pumilus bacteria suspension concentration is 1.8 × 10
7individual/ml, Bifidobacterium bacteria suspension concentration is 2.2 × 10
8individual/ml, lactobacillus reuteri bacteria suspension concentration is 2.8 × 10
6individual/ml, Lactobacillus plantarum bacteria suspension concentration is 3.2 × 10
7individual/ml.
3. a kind of effective pig's feed additive improving intestinal health and improve immunity according to right 1-2, is characterized in that the preparation method of refining burdock root powder is:
(1) fresh radix bardanae is removed the peel, chopping, be placed in air dry oven and dry, make its moisture lower than 12%, pulverize, cross 60 mesh sieves, obtain burdock root powder;
(2) by burdock root powder and complex enzyme formulation, sodium chloride, magnesium chloride 100:(0.5-1 by weight): (0.5-0.8): (0.5-1) mixes, afterwards again by solid-liquid ratio 1:(15-20) add the ethanol of 50-60%, soak 3-5h, soaking temperature is 40-45 DEG C, filter, get filter residue; Described complex enzyme formulation is by cellulase, hemicellulase and pectase 3:(1-2 by weight): (1-3) mixes;
(3) filter residue step (2) obtained is dried, then by solid-liquid ratio 1:(10-20) adding distilled water, refluxing extraction 2-3h, Extracting temperature is 35-40 DEG C, filters, gets filtrate, repeat extraction 2 times, merging filtrate;
(4) by filtrate concentrate drying, pulverized 60 mesh sieves, obtained refining burdock root powder, preserved at-20 DEG C of temperature lower seals.
4. a kind of effective pig's feed additive improving intestinal health and improve immunity according to claim 1-2, is characterized in that the preparation method of Caulis Lonicerae extract is:
(1) caulis lonicerae of drying is pulverized, cross 60 mesh sieves, with hemicellulase and pectase 100:(0.3-0.5 by weight): (0.5-1) mixes, then by solid-liquid ratio 1:(5-10) add distilled water, soak 3-5h, soaking temperature is 5-10 DEG C, soak complete filtration afterwards, get filter residue, dry, make its moisture lower than 15%, bake out temperature is 60-65 DEG C;
(2) by filter residue and the aluminum sulfate after drying and crab shell powder by weight 50:(1-3): (1-2) mix, by solid-liquid ratio 1:(5-8) add distilled water, immersion 2-4h, soaking temperature is 5-10 DEG C, and filtration, gets filter residue;
(3) filter residue is dried, by solid-liquid ratio 1:(10-15) add the ethanol of 60-70%, refluxing extraction 2-3h, Extracting temperature is 40-50 DEG C, gets filtrate, and filter residue extracts 1 time again, merges the filtrate of 2 extractions;
(4) filtrate is concentrated, spraying dry, pulverized 60 mesh sieves, and obtained Caulis Lonicerae extract.
5. a kind ofly according to claim 3 effectively improve intestinal health and improve the pig's feed additive of immunity, it is characterized in that the preparation method of refining burdock root powder is:
(1) fresh radix bardanae is removed the peel, chopping, be placed in air dry oven and dry, make its moisture lower than 12%, pulverize, cross 60 mesh sieves, obtain burdock root powder;
(2) burdock root powder and complex enzyme formulation, sodium chloride, magnesium chloride are mixed by weight 100:0.6:0.6:0.8, add the ethanol of 55% afterwards again by solid-liquid ratio 1:18, soak 4h, soaking temperature is 42 DEG C, filters, gets filter residue; Described complex enzyme formulation is mixed by weight 3:1.5:2 by cellulase, hemicellulase and pectase;
(3) filter residue step (2) obtained is dried, then adds distilled water by solid-liquid ratio 1:15, refluxing extraction 2.5h, and Extracting temperature is 36 DEG C, filters, gets filtrate, repeat extraction 2 times, merging filtrate;
(4) by filtrate concentrate drying, pulverized 60 mesh sieves, obtained refining burdock root powder, preserved at-20 DEG C of temperature lower seals.
6. a kind ofly according to claim 4 effectively improve intestinal health and improve the pig's feed additive of immunity, it is characterized in that the preparation method of Caulis Lonicerae extract is:
(1) caulis lonicerae of drying is pulverized, cross 60 mesh sieves, mix by weight 100:0.4:0.8 with hemicellulase and pectase, then add distilled water by solid-liquid ratio 1:8, soak 4h, soaking temperature is 8 DEG C, soak complete filtration afterwards, get filter residue, dry, make its moisture lower than 15%, bake out temperature is 62 DEG C;
(2) filter residue and the aluminum sulfate after drying and crab shell powder mixs by weight 50:2:1.5, add distilled water by solid-liquid ratio 1:6, immersion 3h, soaking temperature is 8 DEG C, and filtration, gets filter residue;
(3) dried by filter residue, add the ethanol of 65% by solid-liquid ratio 1:12, refluxing extraction 2.5h, Extracting temperature is 45 DEG C, gets filtrate, and filter residue extracts 1 time again, merges the filtrate of extracting for 2 times;
(4) filtrate is concentrated, spraying dry, pulverized 60 mesh sieves, and obtained Caulis Lonicerae extract.
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