CN106721487A - A kind of grouper fermentation of Chinese herbal medicine immunopotentiator - Google Patents
A kind of grouper fermentation of Chinese herbal medicine immunopotentiator Download PDFInfo
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- CN106721487A CN106721487A CN201510778843.9A CN201510778843A CN106721487A CN 106721487 A CN106721487 A CN 106721487A CN 201510778843 A CN201510778843 A CN 201510778843A CN 106721487 A CN106721487 A CN 106721487A
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- fermentation
- grouper
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention discloses a kind of grouper fermentation of Chinese herbal medicine immunopotentiator, with diseases prevention, cures the disease and strengthen the multiple efficacies such as immunity of organisms.It is the preferable of substitute antibiotics class medicine, safety, green, nontoxic novel fodder additive.By compound Chinese herb, by " aerobic and anaerobism asynchronous fermentation ", this mixed fungus fermentation technique is obtained the fermentation of Chinese herbal medicine feed addictive, and mixed fungus fermentation can make various bacterium collaborations produce bigger advantage, make up the weak point of single strain fermentation.Obtained product has the two-fold advantage of Chinese herbal medicine and probiotics, can effectively nurse one's health grouper intestinal health, keeps intestinal microecology balance and optimal immune state, improves the digestibility of nutriment；Stimulate grouper nonspecific immunity mechanism, strengthen its immune response ability；Cultivation income is improved, cultivation risk is reduced.The present invention is used as antibiotic substitute, without resistance and the problems such as medicament residue, and grouper is had no adverse effects, and uses safety.
Description
Technical field
The present invention relates to field of feed additive technology, especially a kind of grouper fermentation of Chinese herbal medicine immunopotentiator.
Background technology
With the continuous improvement of living standards of the people, people for safety, green, the demand of healthy agricultural product, be also with
Day all increasings.Therefore how safe and nontoxic, green, environmentally friendly additive is used in cultivation, as research institutions at present
One of hot issue of research.Wherein, the use of Chinese herb feed additive is recognized by many professionals.Chinese herbal medicine
Feed addictive has the advantages that hypotoxicity, without the resistance to the action of a drug, multi-functional, be used widely in husbandry sector.
And microorganism plays powerful decomposition and inversion ability in Chinese herbal medicine fermentation process, and abundant secondary metabolism can be produced to produce
Thing, can greatly improve the content of active component in Chinese herbal medicine, improve curative effect, reduce toxic and side effect, and this is for focusing on cultivating
The culture fishery of cost is particularly important.
Grouper, belongs to Perciformes, and body oblong is slightly flat-sided, and variation in body color is a lot of, normal brown or red, and has bar
Line and spot, are the big-and-middle-sized seafood fish of water warm.Grouper can be divided into many types：Extra large erythema, eastern star spot, west
Star spot, safe star spot, leopard star spot, mouse spot, tiger spot, blue spot etc..Mainly blue spot, the tiger propagated artificially at present
Spot.Grouper is nutritious because of its, and fine and tender taste is pure white, is similar to chicken, have the title of " extra large chicken ".Grouper is again
It is the first-class food fish of a kind of low fat, high protein, it is one of four big fishes of China to be pushed away by Hong Kong and Macao, in Japan, newly
It is the improved seeds of foreign exchange earning plus also there is huge market potential in the country such as slope, Malaysia.Due to natural yield
Deficiency, propagating grouper artificially has turned into the topmost approach for meeting the grouper market demand.But in its breeding process
The drawback such as existing antibiotic usage is excessive, cultivation risk is big turns into the bottleneck factor of its development of restriction.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art, there is provided a kind of green, environmentally friendly, efficient grouper fermented tcm
Immunopotentiator.
The present invention is adopted the technical scheme that：
A kind of grouper fermentation of Chinese herbal medicine immunopotentiator, it is characterised in that compound Chinese herb by following mass ratio Chinese herbal medicine group
Into：15~35 parts of camellia seed, 20~35 parts of the Radix Astragali, 10~25 parts of hawthorn, 6~10 parts of Radix Glycyrrhizae, 6~8 parts of barrenwort,
8~15 parts of raw cape jasmine, 15~28 parts of the Chinese bulbul, 5~7 parts of Radix Angelicae Sinensis, 3~5 parts of Radix Codonopsis.
The present invention provides a kind of grouper fermentation of Chinese herbal medicine immunopotentiator, and (China is general for black bent probiotic strain for selected leavening
Logical microbial strains preservation administrative center, bacterium numbering CGMCC3.3289) and Lactobacillus plantarum (China General Microbiological bacterial strain
Preservation administrative center, bacterium numbering CGMCC1.124) composition composite ferment, wherein the inoculum concentration of black bent probiotic strain be 2~
4%, the inoculum concentration of Lactobacillus plantarum is 3~5%.
The present invention provides a kind of preparation method of grouper fermentation of Chinese herbal medicine immunopotentiator, specifically includes following steps：
(1) preparation of compound Chinese herb：According to above-mentioned formula precise Chinese herbal medicine, 80 mesh are crushed to, are well mixed,
Compound Chinese herb is obtained；
(2) extraction of Traditional Chinese medicine water-soluble active material and sterilizing：To the distilled water that 5~10 times are added in compound Chinese herb pulvis, plus
Enter glucose 1~2%, yeast extract 0.1~0.4%, K2HPO40.05~0.2%, pH is to 4.5~5.5 for regulation, it is warming up to 95~
100 DEG C are soaked 3 hours；
(3) aerobic fermentation：From 50L liquid fermentation tanks, liquid amount is 60~70%, inoculated aspergillus niger probiotic strain, inoculation
It is 2~4% to measure, 30~40 DEG C of fermentation temperature of control, 150~300rpm of rotating speed, 10~20L/min of throughput, with this understanding
48~72h of fermentation；
(4) anaerobic fermentation：After aerobic fermentation terminates, inoculated plant lactobacillus, inoculum concentration be 3~5%, fermentation temperature be 35~
45 DEG C, ferment 24~48h；
(5) fermentation post processing：Fermented liquid is adsorbed with 1~2 times of volume ratio wheat bran after the completion of fermentation, in 40~60 DEG C of air blast
Dried in drying box, moisture is controlled 7~10%；
(6) crush：Dried product was crushed into 80 mesh sieves, grouper fermentation of Chinese herbal medicine immunopotentiator was obtained final product.
The present invention provides a kind of grouper fermentation of Chinese herbal medicine immunopotentiator, and the grouper is produced with fermentation of Chinese herbal medicine immunopotentiator
Product mass ratio used in aquatic feeds complete diet pellet is 0.2~1.0%；Or described feed addictive is in premix, concentration
Expect to be used with the ratio addition needed for complete diet pellet.
Advantages and positive effects of the present invention are：
It is and existing using " " aerobic and anaerobism asynchronous fermentation " mixed fungus fermentation technique in fermentation of Chinese herbal medicine feed addictive preparation process
The similar Chinese herbal feed additive product having is more notable compared to action effect；Fermentation of Chinese herbal medicine feed addictive have Chinese herbal medicine and
The two-fold advantage of probiotics, can protect grouper intestinal health, keep intestinal microecology balance and optimal immune state, improve battalion
Support the digestibility of material；Stimulate grouper nonspecific immunity mechanism, strengthen its immune response ability；Cultivation income is improved,
Reduce cultivation risk.The present invention is used as antibiotic substitute, without resistance and the problems such as medicament residue, and to grouper invariably
Good influence, uses safety.
Specific embodiment
With reference to embodiment, the present invention is further described, and following embodiments are illustrative, is not limited, it is impossible to
Protection scope of the present invention is limited with following embodiments.
Embodiment 1
A kind of grouper fermentation of Chinese herbal medicine immunopotentiator, specific preparation process is as follows：
(1) weigh：According to following formula precise Chinese herbal medicine：25 parts of camellia seed, 20 parts of the Radix Astragali, 10 parts of hawthorn, Radix Glycyrrhizae
7 parts, 6 parts of barrenwort, 10 parts of raw cape jasmine, 20 parts of the Chinese bulbul, 5 parts of Radix Angelicae Sinensis, 5 parts of Radix Codonopsis.
(2) preparation of compound Chinese herb：Chinese herbal medicine powder after weighing is broken to 80 mesh, is well mixed, compound Chinese herb is obtained；
(3) extraction of Traditional Chinese medicine water-soluble active material and sterilizing：To the distilled water that 8 times are added in compound Chinese herb pulvis, Portugal is added
Grape sugar 1%, yeast extract 0.2%, K2HPO40.05%, pH is to 5.0 for regulation, is warming up to 95~100 DEG C and soaks 3 hours；
(4) aerobic fermentation：From 50L liquid fermentation tanks, liquid amount is 60~70%, inoculated aspergillus niger probiotic strain, inoculation
It is 2% to measure, and controls 30 DEG C of fermentation temperature, and 150~300rpm of rotating speed, 10~20L/min of throughput ferment 64 with this understanding；
(5) anaerobic fermentation：After aerobic fermentation terminates, inoculated plant lactobacillus, inoculum concentration is 3%, and fermentation temperature is 37 DEG C, hair
Ferment 32h；
(6) fermentation post processing：Fermented liquid is adsorbed with 1.5 times of volume ratio wheat brans after the completion of fermentation, it is dry in 40~60 DEG C of air blast
Dried in dry case, moisture is controlled 7~10%；
(7) crush：Dried product was crushed into 80 mesh sieves, grouper fermentation of Chinese herbal medicine immunopotentiator was obtained final product.
Embodiment 2
A kind of grouper fermentation of Chinese herbal medicine immunopotentiator, specific preparation process is as follows：
(1) weigh：According to following formula precise Chinese herbal medicine：20 parts of camellia seed, 25 parts of the Radix Astragali, 15 parts of hawthorn, Radix Glycyrrhizae
10 parts, 8 parts of barrenwort, 12 parts of raw cape jasmine, 25 parts of the Chinese bulbul, 7 parts of Radix Angelicae Sinensis, 5 parts of Radix Codonopsis.
(2) preparation of compound Chinese herb：Chinese herbal medicine powder after weighing is broken to 80 mesh, is well mixed, compound Chinese herb is obtained；
(3) extraction of Traditional Chinese medicine water-soluble active material and sterilizing：To the distilled water that 10 times are added in compound Chinese herb pulvis, Portugal is added
Grape sugar 1.5%, yeast extract 0.2%, K2HPO40.1%, pH is to 5.2 for regulation, is warming up to 95~100 DEG C and soaks 3 hours；
(4) aerobic fermentation：From 50L liquid fermentation tanks, liquid amount is 60~70%, inoculated aspergillus niger probiotic strain, inoculation
It is 2% to measure, and controls 32 DEG C of fermentation temperature, and 150~300rpm of rotating speed, 10~20L/min of throughput ferment 60 with this understanding
(5) anaerobic fermentation：After aerobic fermentation terminates, inoculated plant lactobacillus, inoculum concentration is 4%, and fermentation temperature is 35 DEG C, hair
Ferment 36h；
(6) fermentation post processing：Fermented liquid is adsorbed with 1.2 times of volume ratio wheat brans after the completion of fermentation, it is dry in 40~60 DEG C of air blast
Dried in dry case, moisture is controlled 7~10%；
(7) crush：Dried product was crushed into 80 mesh sieves, grouper fermentation of Chinese herbal medicine immunopotentiator was obtained final product.
Embodiment 3
A kind of grouper fermentation of Chinese herbal medicine immunopotentiator, specific preparation process is as follows：
(1) weigh：According to following formula precise Chinese herbal medicine：30 parts of camellia seed, 20 parts of the Radix Astragali, 15 parts of hawthorn, Radix Glycyrrhizae
10th, barrenwort 6, raw cape jasmine 10, the Chinese bulbul 15, Radix Angelicae Sinensis 6, Radix Codonopsis 5.
(2) preparation of compound Chinese herb：Chinese herbal medicine powder after weighing is broken to 80 mesh, is well mixed, compound Chinese herb is obtained；
(3) extraction of Traditional Chinese medicine water-soluble active material and sterilizing：To the distilled water that 6 times are added in compound Chinese herb pulvis, Portugal is added
Grape sugar 1.2%, yeast extract 0.1, K2HPO40.15%, pH is to 5.0 for regulation, is warming up to 95~100 DEG C and soaks 3 hours；
(4) aerobic fermentation：From 50L liquid fermentation tanks, liquid amount is 60~70%, inoculated aspergillus niger probiotic strain, inoculation
It is 2% to measure, 30 DEG C of fermentation temperature of control, 150~300rpm of rotating speed, 10~20L/min of throughput, and ferment 70h with this understanding；
(5) anaerobic fermentation：After aerobic fermentation terminates, inoculated plant lactobacillus, inoculum concentration is 3%, and fermentation temperature is 37 DEG C, hair
Ferment 26h；
(6) fermentation post processing：Fermented liquid is adsorbed with 2 volume ratio wheat brans after the completion of fermentation, in 40~60 DEG C of air dry ovens
Middle drying, moisture is controlled 7~10%；
(7) crush：Dried product was crushed into 80 mesh sieves, grouper fermentation of Chinese herbal medicine immunopotentiator was obtained final product.
To verify the effect of immunopotentiator of the present invention, verified by tests below.
Experimental condition and management：The tail of grouper 480 of health is chosen, body weight is 55 ± 5g, temporarily after foster 14d, be randomly divided into four
Group, i.e. control group, test 1 group, test 2 groups, test 3 groups, every group of 3 repetitions, every group of 40 tails.Control group fed base
Plinth feed；The basal feed that the immunopotentiator of embodiment 1 is added in 1 group of feeding is tested, addition is 0.5%；Test 2 groups of feedings
The basal feed of the immunopotentiator of the embodiment of the present invention 2 is added with, addition is 0.5%；Test 3 groups of feedings and be added with the present invention
The basal feed of the immunopotentiator of embodiment 3, addition is 0.5%.Continuously feed 42 days, feed intake 3 times, feeding rate daily
It is 2%.After off-test, tail vein blood determines Biochemical Indices In Serum, the results are shown in Table 1.
Water temperature during experiment has circulation filter pump and inflator pump at 26.5~28.4 DEG C, refreshes the water periodically and clean filter pulp, maintains molten
In 5.8~7.6mg/L, 6.3~7.8, the concentration of control ammonia (NH3) is less than 0.02mg/L to pH value to solution oxygen, controls nitrous acid
Salt (NO2-) content be less than 0.20mg/L.
Influence of the fermentation of Chinese herbal medicine immunopotentiator of table 1 to grouper Biochemical Indices In Serum
As shown in Table 1, the antalzyme activity and alkaline phosphatase of fermentation of Chinese herbal medicine additive group are above control group；Fermentation of Chinese herbal medicine
The content of ALT, AST, GRE of additive group is below control group.Can reduce in fish body.Illustrate to add fermentation medium-height grass in feed
Medicine immunopotentiator has to the activity of glutamic-pyruvic transaminase, glutamic-oxalacetic transaminease reduces inducing action well, can also stimulate immunity of organism
Function, improves antalzyme activity, strengthens the immunity of grouper.
Claims (2)
1. a kind of grouper fermented tcm immunopotentiator, it is characterised in that by compound Chinese herb by the way that " aerobic+anaerobism is asynchronous
Fermentation " technique is obtained.
2. compound Chinese herb according to claim 1, it is characterised in that be made up of the Chinese herbal medicine of following mass ratio：Camellia seed
15~35 parts, 20~35 parts of the Radix Astragali, 10~25 parts of hawthorn, 6~10 parts of Radix Glycyrrhizae, 6~8 parts of barrenwort, raw cape jasmine 8~15
Part, 15~28 parts of the Chinese bulbul, 5~7 parts of Radix Angelicae Sinensis, 3~5 parts of Radix Codonopsis.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107996889A (en) * | 2017-12-26 | 2018-05-08 | 广西金海环岛渔业有限公司 | Improve the cultural method of grouper survival rate |
| CN109363004A (en) * | 2018-12-20 | 2019-02-22 | 江苏省农业科学院宿迁农科所 | The preparation method and application of big squama Barb fish fermented type Chinese medicine immunity enhancer |
| CN113558151A (en) * | 2020-04-29 | 2021-10-29 | 广西百跃农牧发展有限公司 | Grouper reinforced feed and preparation method thereof |
| CN115624114A (en) * | 2022-09-16 | 2023-01-20 | 贵州省水产研究所 | Sturgeon coated fermented Chinese herbal medicine preparation and preparation method thereof |
-
2015
- 2015-11-13 CN CN201510778843.9A patent/CN106721487A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107996889A (en) * | 2017-12-26 | 2018-05-08 | 广西金海环岛渔业有限公司 | Improve the cultural method of grouper survival rate |
| CN109363004A (en) * | 2018-12-20 | 2019-02-22 | 江苏省农业科学院宿迁农科所 | The preparation method and application of big squama Barb fish fermented type Chinese medicine immunity enhancer |
| CN113558151A (en) * | 2020-04-29 | 2021-10-29 | 广西百跃农牧发展有限公司 | Grouper reinforced feed and preparation method thereof |
| CN115624114A (en) * | 2022-09-16 | 2023-01-20 | 贵州省水产研究所 | Sturgeon coated fermented Chinese herbal medicine preparation and preparation method thereof |
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