CN103621825B - Preparation method and application of fermented Chinese herbal medicine immune enhancer for yellow-head catfish meat - Google Patents
Preparation method and application of fermented Chinese herbal medicine immune enhancer for yellow-head catfish meat Download PDFInfo
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Abstract
The invention discloses a fermented Chinese herbal medicine immune enhancer for yellow-head catfish meat. The fermented Chinese herbal medicine immune enhancer has a plurality of effects of prevention, health-care, immunoregulation and the like, and is an ideal, green, environment-friendly, safe and novel feed additive for replacing an antibiotic medicine. The fermented Chinese herbal medicine immune enhancer is prepared from Chinese herbal compounds by technologies of ultrasonic assisted extraction and multi-strain two-step fermentation, has double advantages of Chinese herbal medicines and probiotics, can efficiently regulate intestinal health of the yellow-head catfish meat, can treat both symptoms and root causes, and can strengthen healthy energy and cultivate the vitality; microecological balance and the best immune state of an intestinal tract are kept; the digestibility of nutrient substances is improved; the body immunity of the yellow-head catfish meat is improved; the farming income is increased; the breeding risk is reduced; the fermented Chinese herbal medicine immune enhancer is used instead of antibiotics, is free of a bad effect on the yellow-head catfish meat, and safe to use, has a broad market prospect, and does not generate the problems such as drug residues and tolerance.
Description
Technical field
The present invention relates to the preparation method of a kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, belong to feed additive field.
Background technology
Aquatic products more and more occupy important share in people's diet, in order to meet the demand of ever-increasing aquatic products, solve because of aquaculture scale constantly expand with growth cycle reduction and cause physiological disorders, disease takes place frequently and becomes the Important Problems of aquaculture, is also the key of aquatic feeds industry development.In this context, antibiotics feed addictive is subject to heat and holds in both hands, and is widely used in feed industry, facilitates the development of culture fishery to a certain extent, but the problem such as high residue, high pollution and the resistance to the action of a drug brought of antibiotic more serious.Current social, food security Frequent Accidents, people also more focus on healthy diet while satisfied having enough to eat and wear, and the aquatic products of green health receive much concern.Chinese herbal feed additive meets the requirement of green aquatic products aquaculture development, eliminates people and to feed out to it safety worries of aquatic products product, should vigorously promote the use.But the research level of current Chinese herbal feed additive is lower, manufacture craft simple coarse, the technological element of a product is lower, and quality is stable not, and problem like this seriously governs applying of Chinese medicine fodder interpolation.Fermentable Chinese medicine is reform to traditional Chinese medicine process technology and innovation, has various features and advantage.This technology is introduced the research of Chinese herbal feed additive, its scientific and technological content can be made significantly to promote, effect is more remarkable, quality is more stable, benefit is more obvious, can immunity of organisms be significantly improved, strengthen the level of aquatic livestock resist the disease, have important using value for the various problems solving aquatic livestock in current culture fishery.Utilize microbial fermentation technology to process Chinese herbal medicine, the content of its main active substances polysaccharide can be made to significantly improve, this seems particularly important for the culture fishery focusing on aquaculture cost.
Pelteobagrus fulvidraco (
pseudobagrus fulvidraco Richardson) also known as loud, high-pitched sound serratd edge, Huang Lading, classification on belong to Silurformes (
silurfoymes) ， Catfish section (
bagridae), Pelteobagrus (
pseudobagrus), this genus has 5 kinds, is respectively Pelteobagrus fulvidraco, pseudobagrus vachelli (Pelteobagrus vachelli), pseudobagrus nitidus, pseudobagrus eupogon and Pelteobagrus intermedius, at present for the mainly Pelteobagrus fulvidraco propagated artificially and pseudobagrus vachelli.Pelteobagrus fulvidraco all has distribution in China's most area rivers and lakes pond, although individuality is less, because its meat younger sister is delicious delicate, few thorn, nutritious, get consumer reception very much, a yellow fish not only has tonic effect, and has certain medical value, and its adult fish is individual general at 100-250g.Also there is huge market potential in country to Huang Shunyu in Japan, Korea S, Southeast Asia etc., is the improved seeds of foreign exchange earning.At present, due to natural underproduce, the Huang Shunyu naturally fished for far can not meet the needs of human lives, propagates the topmost approach becoming Pelteobagrus fulvidraco and meet the need of market artificially.But in Culture of Pelteobagrus Fulvidraco process, also there is the drawbacks such as antibiotic usage is excessive, cultivation has a big risk, become the bottleneck factor of restriction Pelteobagrus fulvidraco aquaculture sustainable development.
Summary of the invention
The object of the invention is to according to above shortcomings in prior art, consider that Chinese herbal medicine has antibacterial, antiviral, improve immunity of organisms and improve the left and right of growth of animal performance, can reduce antibiotic uses the aquaculture caused to pollute in a large number, and raw material sources are wide, cheap, be a kind of green, environmental protection, efficiently feed addictive, develop a kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
A kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, is characterized in that compound Chinese herb is made up of the Chinese herbal medicine of following mass ratio: old 12 ~ 18 parts of bacterium, golden cypress 8 ~ 12 parts, matrimony vine 8 ~ 12 parts, honeysuckle 6 ~ 10 parts, glossy ganoderma 3 ~ 5 parts, milk thistle 6 ~ 10 parts, radix bupleuri 6 ~ 8 parts, the coptis 3 ~ 5 parts, rhizoma alismatis 6 ~ 10 parts, the root of kudzu vine 6 ~ 10 parts, purslane 6 ~ 10 parts, the fleece-flower root 5 ~ 7 parts, SEMEN ASTRAGALI COMPLANATI 3 ~ 5 parts.
The invention provides a kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, selected leavening is by bacillus subtilis (Chinese agriculture Microbiological Culture Collection administrative center, bacterium numbering ACCC10619), S. cervisiae (Angel Yeast), lactobacillus bulgaricus (Chinese agriculture Microbiological Culture Collection administrative center, bacterium numbering ACCC10638) and bifidobacterium bifidum (ATCC standard bacterial classification, bacterium numbering ATCC29521) composite ferment that forms, wherein bacillus subtilis, lactobacillus bulgaricus, the inoculum concentration of bifidobacterium bifidum is 2 ~ 4%, S. cervisiae inoculum concentration is 5 ~ 8%.
The invention provides the preparation method of a kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, specifically comprise the following steps:
(1) preparation of compound Chinese herb: according to above-mentioned formula precise Chinese herbal medicine, be crushed to 40 ~ 60 orders, mix, obtained compound Chinese herb;
(2) Ultrasonic Wave-Assisted Extraction: the water adding 5 ~ 10 times in compound Chinese herb pulvis, under ultrasonic assistant effect, reflux under 100 DEG C of conditions lixiviate 20 ~ 30min;
(3) zymotic fluid preparation: add 3.0 ~ 5.0% soluble starches, 2.0 ~ 3.0% beancake powders, 0.3 ~ 0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(4) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60 ~ 70%, inoculation bacillus subtilis and S. cervisiae, and inoculum concentration is respectively 2 ~ 4%, 5 ~ 8%, controls fermentation temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, throughput 10 ~ 20L/min; At this fermentation condition bottom fermentation 12 ~ 24h;
(5) anaerobic fermentation: after aerobic fermentation terminates, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is 2 ~ 4%, fermentation temperature 28 ~ 37 DEG C, temperature control anaerobic fermentation 12 ~ 24h;
(6) fermentation post processing: after having fermented, fermented liquid 1.2 ~ 2 volume ratio wheat brans are adsorbed, dry in 40 ~ 60 DEG C of blast driers, moisture controls 8 ~ 10%;
(7) pulverize: pulverizing of being sieved by dried product pulverizer 60 order, obtains Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer.
The invention provides a kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, having the multiple efficacies such as prevention, health care, treatment and immunity moderation, is the ideal of substitute antibiotics medicine, green, environmental protection, safety, novel fodder additive.This invention fermentation of Chinese herbal medicine feed addictive one can efficiently nurse one's health Pelteobagrus fulvidraco intestinal health, treats both principal and secondary aspect of disease, reinvigoration, keeps intestinal microecology balance and best immune state, improve the digestibility of nutriment, improve animal body immunity; Two is the generations that can suppress and stop the harmful bacterias such as cause of disease coliform, clostridium, salmonella, increases beneficial bacteria of intestinal tract, maintains healthy gut flora; Three is strengthen immunization, by stimulating siberian crabapple cell in enteron aisle, increasing the formation of local antibody, increasing macrophage activity; Four is that fermentation of Chinese herbal medicine feed addictive enters withstand deleterious bacterium in intestines, reduces stench and harmful components formation, makes the ight soil of discharge eliminate stench, while protection breeding environment, improves Pelteobagrus fulvidraco meat quality.
The invention provides a kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, the mass ratio that described Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer product uses in aquatic feeds complete diet pellet is 0.1 ~ 0.5: or the ratio interpolation use that described feed addictive is required in premix, concentrate feed are with complete diet pellet.
The invention provides a kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, it is advantageous that: one is the production technology that have employed " Chinese herbal medicine Ultrasonic Wave-Assisted Extraction+multi-cultur es two-step fermentation " in fermentation of Chinese herbal medicine feed addictive preparation process, and action effect is more remarkable compared with existing Chinese herbal feed additive product; Two is two-fold advantage that fermentation of Chinese herbal medicine feed addictive has Chinese herbal medicine and probio, can efficiently nurse one's health Pelteobagrus fulvidraco intestinal health, treat both principal and secondary aspect of disease, reinvigoration, keep intestinal microecology balance and best immune state, improve the digestibility of nutriment, improve animal body immunity, improve cultivation income, reduce cultivation risk; Three is the alternative antibiotic usage of this product, has no adverse effects to Pelteobagrus fulvidraco, and use safety, does not exist the problem such as medicament residue and drug resistance, has wide market prospects.
Detailed description of the invention
embodiment 1:
A kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, concrete preparation process is as follows:
(1) weigh: according to following formula precise Chinese herbal medicine: old 12 parts of bacterium, golden cypress 12 parts, matrimony vine 8 parts, honeysuckle 10 parts, glossy ganoderma 3 parts, milk thistle 10 parts, radix bupleuri 6 parts, the coptis 5 parts, rhizoma alismatis 6 parts, the root of kudzu vine 10 parts, purslane 6 parts, the fleece-flower root 7 parts, SEMEN ASTRAGALI COMPLANATI 3 parts;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40 ~ 60 orders, mixes, obtained compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: the water adding 5 times in compound Chinese herb pulvis, under ultrasonic assistant effect, reflux under 100 DEG C of conditions lixiviate 20 ~ 30min;
(4) zymotic fluid preparation: add 3.0% soluble starch, 3.0% beancake powder, 0.3% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(5) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60 ~ 70%, inoculation bacillus subtilis and S. cervisiae, and inoculum concentration is respectively 2%, 8%, controls fermentation temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, throughput 10 ~ 20L/min; At this fermentation condition bottom fermentation 12h;
(6) anaerobic fermentation: after aerobic fermentation terminates, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is respectively 2%, 4%, fermentation temperature 28 ~ 37 DEG C, temperature control anaerobic fermentation 24h;
(7) fermentation post processing: after having fermented, fermented liquid 1.2 times of volume ratio wheat brans are adsorbed, dry in 40 ~ 60 DEG C of blast driers, moisture controls 8 ~ 10%;
(8) pulverize: pulverizing of being sieved by dried product pulverizer 60 order, obtains Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer.
embodiment 2:
A kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, concrete preparation process is as follows:
(1) weigh: according to following formula precise Chinese herbal medicine: old 18 parts of bacterium, golden cypress 8 parts, matrimony vine 12 parts, honeysuckle 6 parts, glossy ganoderma 5 parts, milk thistle 6 parts, radix bupleuri 8 parts, the coptis 3 parts, rhizoma alismatis 10 parts, the root of kudzu vine 6 parts, purslane 10 parts, the fleece-flower root 5 parts, SEMEN ASTRAGALI COMPLANATI 5 parts;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40 ~ 60 orders, mixes, obtained compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: the water adding 10 times in compound Chinese herb pulvis, under ultrasonic assistant effect, reflux under 100 DEG C of conditions lixiviate 20 ~ 30min;
(4) zymotic fluid preparation: add 5.0% soluble starch, 2.0% beancake powder, 0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(5) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60 ~ 70%, inoculation bacillus subtilis and S. cervisiae, and inoculum concentration is respectively 4%, 5%, controls fermentation temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, throughput 10 ~ 20L/min; At this fermentation condition bottom fermentation 24h;
(6) anaerobic fermentation: after aerobic fermentation terminates, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is respectively 4%, 2%, fermentation temperature 28 ~ 37 DEG C, temperature control anaerobic fermentation 12h;
(7) fermentation post processing: after having fermented, fermented liquid 2 times of volume ratio wheat brans are adsorbed, dry in 40 ~ 60 DEG C of blast driers, moisture controls 8 ~ 10%;
(8) pulverize: pulverizing of being sieved by dried product pulverizer 60 order, obtains Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer.
embodiment 3:
A kind of Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, concrete preparation process is as follows:
(1) weigh: according to following formula precise Chinese herbal medicine: old 15 parts of bacterium, golden cypress 10 parts, matrimony vine 10 parts, honeysuckle 8 parts, glossy ganoderma 4 parts, milk thistle 8 parts, radix bupleuri 7 parts, the coptis 4 parts, rhizoma alismatis 8 parts, the root of kudzu vine 8 parts, purslane 8 parts, the fleece-flower root 6 parts, SEMEN ASTRAGALI COMPLANATI 4 parts;
(2) preparation of compound Chinese herb: the Chinese herbal medicine powder after weighing is broken to 40 ~ 60 orders, mixes, obtained compound Chinese herb;
(3) Ultrasonic Wave-Assisted Extraction: the water adding 7.5 times in compound Chinese herb pulvis, under ultrasonic assistant effect, reflux under 100 DEG C of conditions lixiviate 20 ~ 30min;
(4) zymotic fluid preparation: add 4.0% soluble starch, 2.5% beancake powder, 0.4% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(5) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60 ~ 70%, inoculation bacillus subtilis and S. cervisiae, and inoculum concentration is respectively 3%, 6.5%, controls fermentation temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, throughput 10 ~ 20L/min; At this fermentation condition bottom fermentation 18h;
(6) anaerobic fermentation: after aerobic fermentation terminates, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is 2 ~ 4%, fermentation temperature 28 ~ 37 DEG C, temperature control anaerobic fermentation 18h;
(7) fermentation post processing: after having fermented, fermented liquid 1.6 times of volume ratio wheat brans are adsorbed, dry in 40 ~ 60 DEG C of blast driers, moisture controls 8 ~ 10%;
(8) pulverize: pulverizing of being sieved by dried product pulverizer 60 order, obtains Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer.
Above embodiment only for illustration of technical scheme of the present invention, but not is limited; Although be described in detail by invention with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or equivalent replacement has been carried out to wherein portion of techniques feature; And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (1)
1. a Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer, be is characterized in that being obtained by " the two-step fermentation technique that Chinese herbal medicine Ultrasonic Wave-Assisted Extraction+multi-cultur es aerobic fermentation and anaerobic fermentation combine ", specifically comprises the following steps:
(1) preparation of compound Chinese herb: according to old 12 ~ 18 parts of bacterium, golden cypress 8 ~ 12 parts, matrimony vine 8 ~ 12 parts, honeysuckle 6 ~ 10 parts, glossy ganoderma 3 ~ 5 parts, milk thistle 6 ~ 10 parts, radix bupleuri 6 ~ 8 parts, the coptis 3 ~ 5 parts, rhizoma alismatis 6 ~ 10 parts, the root of kudzu vine 6 ~ 10 parts, purslane 6 ~ 10 parts, the fleece-flower root 5 ~ 7 parts, SEMEN ASTRAGALI COMPLANATI 3 ~ 5 parts of ratio precise Chinese herbal medicines, be crushed to 40 ~ 60 orders, mix, obtained compound Chinese herb;
(2) Ultrasonic Wave-Assisted Extraction: the water adding 5 ~ 10 times in compound Chinese herb pulvis, under ultrasonic assistant effect, reflux under 100 DEG C of conditions lixiviate 20 ~ 30min;
(3) zymotic fluid preparation: add 3.0 ~ 5.0% soluble starches, 2.0 ~ 3.0% beancake powders, 0.3 ~ 0.5% potassium dihydrogen phosphate, 0.02%MgSO in the Chinese herbal medicine leaching liquor after lixiviate
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(4) aerobic fermentation: select 50L liquid fermentation tank, liquid amount is 60 ~ 70%, inoculation bacillus subtilis and S. cervisiae, and inoculum concentration is respectively 2 ~ 4%, 5 ~ 8%, controls fermentation temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, throughput 10 ~ 20L/min; At this fermentation condition bottom fermentation 12 ~ 24h;
(5) anaerobic fermentation: after aerobic fermentation terminates, inoculation lactobacillus bulgaricus and bifidobacterium bifidum, inoculum concentration is 2 ~ 4%, fermentation temperature 28 ~ 37 DEG C, temperature control anaerobic fermentation 12 ~ 24h;
(6) fermentation post processing: after having fermented, fermented liquid 1.2 ~ 2 volume ratio wheat brans are adsorbed, dry in 40 ~ 60 DEG C of blast driers, moisture controls 8 ~ 10%;
(7) pulverize: pulverizing of being sieved by dried product pulverizer 60 order, obtains Pelteobagrus fulvidraco fermented type Chinese medicine immunity enhancer.
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| CN104222675B (en) * | 2014-09-30 | 2016-06-08 | 青岛嘉瑞生物技术有限公司 | The preparation method of a kind of bastard halibut and plaice Chinese medicine immunity enhancer and application |
| CN104543372B (en) * | 2015-01-18 | 2017-09-29 | 新疆瑞源丰贸易有限公司 | A kind of sunflower disk mixed feed and preparation method thereof |
| CN104957437A (en) * | 2015-07-29 | 2015-10-07 | 青岛海之星生物科技有限公司 | Compound feed for Pelteobagrus fulvidraco and preparation method of compound feed |
| CN105265376B (en) * | 2015-09-15 | 2018-10-19 | 广东海洋大学 | A kind of Chinese herbal formula promoting a variety of probiotics in-vitro multiplications |
| CN105660470A (en) * | 2016-01-18 | 2016-06-15 | 广德县清元水产养殖专业合作社 | Method for ecological breeding of pelteobagrus fulvidraco |
| CN105660471A (en) * | 2016-01-18 | 2016-06-15 | 广德县清元水产养殖专业合作社 | Method for small-area breeding of pelteobagrus fulvidraco |
| CN106615996A (en) * | 2016-09-18 | 2017-05-10 | 广西大学 | Traditional Chinese medicine feed additive capable of improving disease resistance of grass carp and preparation method of traditional Chinese medicine feed additive |
| CN106721663A (en) * | 2016-12-28 | 2017-05-31 | 青岛嘉瑞生物技术有限公司 | A kind of method that bioanalysis prepares cobio expanding compound feed using glasswort |
| CN106819655A (en) * | 2017-02-17 | 2017-06-13 | 浙江万里学院 | A kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield and its preparation application method |
| CN108606165B (en) * | 2018-04-19 | 2022-02-11 | 广东联鲲集团有限公司 | Additive for improving intestinal sclerosis of pelteobagrus fulvidraco and application thereof |
| CN108782955A (en) * | 2018-07-12 | 2018-11-13 | 漳州傲农牧业科技有限公司 | A kind of feed and preparation method thereof improving Pelteobagrus fulvidraco body colour and meat |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072322A (en) * | 1991-11-20 | 1993-05-26 | 申元刚 | Chinese herbal feed additive |
| CN101116473A (en) * | 2006-08-01 | 2008-02-06 | 济南亿民动物药业有限公司 | Method for preparing glycolysis Chinese herbal medicine preparations for feed |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072322A (en) * | 1991-11-20 | 1993-05-26 | 申元刚 | Chinese herbal feed additive |
| CN101116473A (en) * | 2006-08-01 | 2008-02-06 | 济南亿民动物药业有限公司 | Method for preparing glycolysis Chinese herbal medicine preparations for feed |
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