CN106819655A - A kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield and its preparation application method - Google Patents
A kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield and its preparation application method Download PDFInfo
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- CN106819655A CN106819655A CN201710085135.6A CN201710085135A CN106819655A CN 106819655 A CN106819655 A CN 106819655A CN 201710085135 A CN201710085135 A CN 201710085135A CN 106819655 A CN106819655 A CN 106819655A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield, it is characterised in that be made up of the raw material of the weight/mass percentage composition of following each component：Gynostemma pentaphylla 6~12%, the coptis 3~5%, sargassum fusifome 4~8%, milk thistle 4 ~ 8%, sweet wormwood 5 ~ 10%, the bark of official magnolia 6 ~ 12%, purslane 10 ~ 15%, dandelion 4 ~ 8%, barrenwort 6 ~ 15%, Radix Isatidis 8 ~ 15%, cordate houttuynia 5 ~ 10%, perilla leaf 5 ~ 10% and Asiatic plantain 4 ~ 8%.The invention also discloses the fermentation of Chinese herbal medicine additive preparation and application for improving oyster seed yield.Herbal raw material source of the present invention is wide, it is cheap, production and processing technology is simple, is soaked using ultrasonic assistant, then carry out aerobic fermentation and anaerobic fermentation, improve Chinese herbal medicine functional components and beneficial microbe composition, with improvement oyster seedling water, effectively suppress the outburst of disease microorganism, strengthen oyster seed young immune disease-resistance ability, the survival rate of seed is improved, is a kind of ideal, green, environmental protection, safety, Novel Chinese herbal medicine additive.
Description
Technical field
The present invention relates to a kind of marine shellfish Chinese herb feed additive, more particularly, to a kind of hair for improving oyster seed yield
Ferment Chinese herb feed additive and its preparation application method.
Background technology
Oyster belongs to Mollusca (Mollusca), Bivalvia (Bivalvia), pearl shell mesh (Pterioida), oyster
Section (Ostreidae), is distributed widely in each marine site in the world, is one of marine fishery resources important all over the world.Its meat fertilizer is refreshing
Sliding, delicious flavour is nutritious, containing nutritions such as rich in protein, fat, calcium, phosphorus, iron, have " seabed milk "
Laudatory title.Oyster also has good food therapy effect.The traditional Chinese medical science think oyster it is sweet it is flat it is nontoxic solve the five internal organs, middle regulating and qi tonifying blood-nourishing is solving
Erysipelas, the promoting blood circulation of quenching the thirst of sobering up is allayed one's hunger, and often to eat also has moisturizing beauty treatment to support to hold function.Modern medicine is additionally considered that oyster meat also has drop blood
The function such as pressure and nourishing yin and nourishing blood.Oyster also has " thin skin, beauty face " and hypotensive and nourishing yin and nourishing blood, the strong body of body-building etc. various
Effect, thus it is considered as delicious Hai Zhen and vigorous and graceful salubrity food.Oyster is the important sea-farming object in countries in the world, it has also become
China or even the maximum economic shellfish of world's cultured output.Consumption demand amount constantly expands, and promotes the rapid of oyster culture industry
Development, the increase to seed demand, artificial breeding is also developed rapidly.But in oyster nursery production, often occur
Incubation rate is relatively low, the problems such as veliger disintegrates dead, influences oyster seed yield.Analyzing main cause is：1. substrate deteriorates
Cause Deng environment discomfort；2. water treatment is improper, and harmful microorganism causes disease to infect.In conventional oyster seedling raising process, pass through
Antibiotic is added in seedling water to suppress pathogen growth, improves the survival rate of seed.But it is easily caused using antibiotic
Disease microorganism develops immunity to drugs, and causes antibiotic to fail；In addition larval metamorphosis deformity and medicine are easily caused using antibiotic
Residual, the seed speed of growth is unhappy in causing later stage breeding process, and yield is not high, has a strong impact on the cultivation income of raiser.Again
Water quality improvement is have ignored in person's seedling raising process, causes inherently improve weed survival rate.
From the point of view of existing patent with paper, relevant control oyster seed disease is had no, improve the Chinese herbal medicine of seed yield
Additive report, Chinese herb feed additive have medicine source extensively, efficiently, the resistance to the action of a drug not significantly, toxic and side effect it is small, moreover it is possible to improve enhancing
The advantages of body, enhance immunity, during aquatic animal disease is prevented and treated, in addition to having the property of medicine and trophism concurrently, also have
Effect of aquatic livestock production performance and efficiency of feed utilization is improved, so be increasingly taken seriously, using also increasingly extensive.Micro- life
Thing fermented tcm additive is the reform and innovation to traditional Chinese medicine process technology, with various features and advantage.By this technology
Chinese herbal medicine improver of water quality is introduced, can significantly improve its main active substances polysaccharide and active beneficial microorganism content, section
Skill content is substantially improved, and effect is more significantly, and quality is more stablized, and benefit is more obvious, can significantly improve immunity of organisms,
Strengthen the level of aquatic livestock resist the disease, improve nursery water quality, for solving many of aquatic livestock in current culture fishery
The problem of kind has important application value.
The content of the invention
It is an object of the invention to the above mentioned problem according to present in existing oyster seedling-raising technique, it is contemplated that Chinese herbal medicine has
Antibacterial, effect that is antiviral, improving immunity of organism and improvement growth of animal performance, and raw material sources are wide, it is cheap, it is one
Green, the additive of bait of high-efficiency environment friendly are planted, develops a kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield to strengthen
The aquaculture that oyster seed immunity of organism, the water quality environment for improving oyster seedling water, reduction metabolin discharge are caused pollutes, subtracts
Survival rate and yield that few oyster seed disease occurs, reduces oyster seed abnormal rate, improving oyster seed, oyster is promoted with this
Aquaculture healthy and sustainable development.
Above-mentioned purpose of the present invention is to be achieved by the following technical programs：
A kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield, it is characterised in that contained by the quality percentage of following each component
The raw material of amount is made：Gynostemma pentaphylla 6~12%, the coptis 3~5%, sargassum fusifome 4~8%, the % of milk thistle 4 ~ 8, sweet wormwood 5 ~ 10%, the bark of official magnolia 6 ~
12%th, purslane 10 ~ 15%, dandelion 4 ~ 8%, barrenwort 6 ~ 15%, Radix Isatidis 8 ~ 15%, cordate houttuynia 5 ~ 10%, the He of perilla leaf 5 ~ 10%
Asiatic plantain 4 ~ 8%.The percentage by weight sum of above-mentioned each component is 100%.
The present invention also provides a kind of preparation and application of the fermentation of Chinese herbal medicine additive for improving oyster seed yield, tool
Body is comprised the following steps：
（1）Get the raw materials ready：Impurity in each traditional Chinese medicinal material raw materials is rejected, it is clear with pure water according to above-mentioned each raw material of formula precise
Dried after washing traditional Chinese medicinal material raw materials 3 ~ 4 times, traditional Chinese medicinal material raw materials are crushed to 50~75 mesh, be well mixed, compound Chinese herb is obtained；
（2）Extraction：To the pure water that 30 ~ 40 times are added in compound Chinese herb raw material, in the effect of 300 ~ 600 W ultrasonic assistants
Under, 95~100 DEG C of extraction temperature extracts 30~60 min.
（3）It is prepared by zymotic fluid：To 1.0~3.0% sucrose of addition, 0.5~1.5% albumen in the Chinese herbal medicine feed liquid after extraction
Peptone, 1.0~2.0% peanut meals, 0.2~0.4% K2HPO4, 0.3~0.5% MgSO4.7H2O, 0.02~0.04% CaCl2With
0.02-0.04 % FeSO4.7H2O, pH are adjusted to 7.0~7. 5.
（4）Aerobic fermentation：From liquid fermentation tank, liquid amount is 50~70%, 2~4% bacillus subtilises of inoculation, 1~
2% bacillus licheniformis and 1~3% S. cervisiae, control 28~37 DEG C of fermentation temperature, the rpm of rotating speed 150~250, throughput
40~60 L/min, the h of fermentation time 30~40.
（5）Anaerobic fermentation：After aerobic fermentation terminates, 1~3% lactobacillus acidophilus of inoculation, 2~4% Bifidobacteriums, 1~2% nitre
Change bacterium, 28~37 DEG C of fermentation temperature, the rpm of rotating speed 150~250, the h of temperature control anaerobic fermentation 15~25.
（6）Fermentation post processing：After the completion of fermentation, cross 120 ~ 160 mesh filter clothes and obtain ferment filtrate, obtain final product fermentation of Chinese herbal medicine addition
Agent.
（7）Usage and dosage：After oyster seedling water changed a water every 2 days, addition seedling water volume 0.1 ~ 0.4%
Fermentation of Chinese herbal medicine additive.
The present invention provides a kind of fermentation of Chinese herbal medicine additive for improving oyster seed rearing, with improvement oyster nursery water
Body, effectively suppresses pathogenic microorganism outburst, strengthens oyster seed immunity and premunition, improves oyster seed larval growth
Can, the multiple efficacies such as the survival rate and yield of oyster seed are improved, it is preferable, green, environmentally friendly, the peace of substitute antibiotics medicine
Entirely, new improver of water quality.Product rear pollution-free, noresidue, without any side effects, safety and environmental protection.Advantage of the invention exists
In：One is that the present invention is using ultrasonic assistant leaching, then carries out aerobic fermentation and anaerobic compared with the simple crushing of Chinese herbal medicine is added
Fermentation, improves Chinese herbal medicine functional components and beneficial microbe composition, can more effectively suppress disease microorganism, improves oyster seed
Young immune disease-resistance ability and survival rate；Two is that raw material sources are wide in the present invention, and cheap, production and processing technology is simple.
Specific embodiment
Below by specific embodiment, the present invention will be further described.In the present invention, if not refering in particular to, Suo Youbai
Point than being unit of weight, all devices and raw material are commercially available or the industry is conventional, in following embodiments
Method, unless otherwise instructed, is this area conventional method.
Embodiment 1
A kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield, by herbal raw material gynostemma pentaphylla, the coptis, sargassum fusifome, water
Fly Ji, sweet wormwood, the bark of official magnolia, purslane, dandelion, barrenwort, Radix Isatidis, cordate houttuynia, perilla leaf and Asiatic plantain composition.Chinese herbal medicine is former
The specific proportioning of each component is as shown in table 1 in material.
A kind of preparation and application of the fermentation of Chinese herbal medicine additive for improving oyster seed rearing, specifically includes following
Step：
（1）Get the raw materials ready：Impurity in each traditional Chinese medicinal material raw materials is rejected, it is clear with pure water according to above-mentioned each raw material of formula precise
Dried after washing traditional Chinese medicinal material raw materials 3 times, traditional Chinese medicinal material raw materials are crushed to 60 mesh, be well mixed, compound Chinese herb is obtained.
（2）Extraction：To the pure water that 5 times are added in compound Chinese herb raw material, under the effect of 400 W ultrasonic assistants, leaching
95 DEG C of temperature raising degree, extracts 40 min.
（3）It is prepared by zymotic fluid：To adding 1.0% sucrose, 1.0% peptone, 2.0% peanut in the Chinese herbal medicine feed liquid after extraction
The dregs of rice, 0.4% K2HPO4、0.4% MgSO4.7H2O、0.03% CaCl2, 0.03 % FeSO4.7H2O, pH are adjusted to 7.2.
（4）Aerobic fermentation：From liquid fermentation tank, liquid amount is 65%, 3% bacillus subtilis of inoculation, 1% lichens gemma
Bacillus and 2% S. cervisiae, control 35 DEG C of fermentation temperature, the rpm of rotating speed 180, the L/min of throughput 50, the h of fermentation time 35.
（5）Anaerobic fermentation：After aerobic fermentation terminates, 2% lactobacillus acidophilus of inoculation, 3% Bifidobacterium and 1% nitrobacteria, hair
32 DEG C of ferment temperature, the rpm of rotating speed 240, temperature control anaerobic fermentation 20h.
（6）Fermentation post processing：After the completion of fermentation, cross 150 mesh filter clothes and obtain ferment filtrate, obtain final product fermentation of Chinese herbal medicine additive.
（7）Usage and dosage：After oyster seedling water changed a water every 2 days, the hair of addition seedling water volume 0.2%
Ferment Chinese herb feed additive.
Embodiment 2
A kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield, by herbal raw material gynostemma pentaphylla, the coptis, sargassum fusifome, water
Fly Ji, sweet wormwood, the bark of official magnolia, purslane, dandelion, barrenwort, Radix Isatidis, cordate houttuynia, perilla leaf and Asiatic plantain composition.Chinese herbal medicine is former
The specific proportioning of each component is as shown in table 1 in material.
A kind of preparation and application of the fermentation of Chinese herbal medicine additive for improving oyster seed rearing, specifically includes following
Step：
（1）Get the raw materials ready：Impurity in each traditional Chinese medicinal material raw materials is rejected, it is clear with pure water according to above-mentioned each raw material of formula precise
Dried after washing traditional Chinese medicinal material raw materials 4 times, traditional Chinese medicinal material raw materials are crushed to 65 mesh, be well mixed, compound Chinese herb is obtained.
（2）Extraction：To the pure water that 40 times are added in compound Chinese herb raw material, under the effect of 500 W ultrasonic assistants, leaching
98 DEG C of temperature raising degree, extracts 50 min.
（3）It is prepared by zymotic fluid：To adding 1.5% sucrose, 1.2% peptone, 1.5% peanut in the Chinese herbal medicine feed liquid after extraction
The dregs of rice, 0.3% K2HPO4、0.3% MgSO4.7H2O、0.02% CaCl2With 0.03 % FeSO4.7H2O, pH are adjusted to 7.0.
（4）Aerobic fermentation：From liquid fermentation tank, liquid amount is 60%, 2% bacillus subtilis of inoculation, 1% lichens gemma
Bacillus and 1% S. cervisiae, control 32 DEG C of fermentation temperature, rotating speed 160 rpm, throughput 40L/min, the h of fermentation time 40.
（5）Anaerobic fermentation：After aerobic fermentation terminates, 2% lactobacillus acidophilus of inoculation, 2% Bifidobacterium and 1~1.5% nitrification are thin
Bacterium, 35 DEG C of fermentation temperature, the rpm of rotating speed 200, the h of temperature control anaerobic fermentation 18.
（6）Fermentation post processing：After the completion of fermentation, cross 120 mesh filter clothes and obtain ferment filtrate, obtain final product fermentation of Chinese herbal medicine additive.
（7）Usage and dosage：After oyster seedling water changed a water every 2 days, the hair of addition seedling water volume 0.3%
Ferment Chinese herb feed additive.
Embodiment 3
A kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield, by herbal raw material gynostemma pentaphylla, the coptis, sargassum fusifome, water
Fly Ji, sweet wormwood, the bark of official magnolia, purslane, dandelion, barrenwort, Radix Isatidis, cordate houttuynia, perilla leaf and Asiatic plantain composition.Chinese herbal medicine is former
The specific proportioning of each component is as shown in table 1 in material.
A kind of preparation and application of the fermentation of Chinese herbal medicine additive for improving oyster seed rearing, specifically includes following
Step：
（1）Get the raw materials ready：Impurity in each traditional Chinese medicinal material raw materials is rejected, it is clear with pure water according to above-mentioned each raw material of formula precise
Dried after washing traditional Chinese medicinal material raw materials 3 times, traditional Chinese medicinal material raw materials are crushed to 70 mesh, be well mixed, compound Chinese herb is obtained；
（2）Extraction：To the pure water that 30 times are added in compound Chinese herb raw material, under the effect of 450 W ultrasonic assistants, extraction temperature
96 DEG C of degree, extracts 45 min.
（3）It is prepared by zymotic fluid：To adding 2.5% sucrose, 0.8% peptone, 2.0% peanut in the Chinese herbal medicine feed liquid after extraction
The dregs of rice, 0.2% K2HPO4、0.4% MgSO4.7H2O、0.04% CaCl2With 0.04 % FeSO4.7H2O, pH are adjusted to 7. 5.
（4）Aerobic fermentation：From liquid fermentation tank, liquid amount is 55%, 4% bacillus subtilis of inoculation, 2% lichens gemma
Bacillus and 3% S. cervisiae, control 30 DEG C of fermentation temperature, the rpm of rotating speed 200, the L/min of throughput 60, the h of fermentation time 30.
（5）Anaerobic fermentation：After aerobic fermentation terminates, 3% lactobacillus acidophilus of inoculation, 2% Bifidobacterium and 2% nitrobacteria, hair
37 DEG C of ferment temperature, the rpm of rotating speed 180, the h of temperature control anaerobic fermentation 16.
（6）Fermentation post processing：After the completion of fermentation, cross 150 mesh filter clothes and obtain ferment filtrate, obtain final product fermentation of Chinese herbal medicine additive.
（7）Usage and dosage：After oyster seedling water changed a water every 2 days, the hair of addition seedling water volume 0.2%
Ferment Chinese herb feed additive.
The composition and ratio of each embodiment herbal raw material of table 1
| Component | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Gynostemma pentaphylla（%） | 9 | 7 | 10 |
| The coptis（%） | 4 | 5 | 3 |
| Sargassum fusifome（%） | 7 | 6 | 4 |
| Milk thistle（%） | 5 | 6 | 7 |
| Sweet wormwood（%） | 7 | 8 | 9 |
| The bark of official magnolia（%） | 8 | 6 | 9 |
| Purslane（%） | 11 | 12 | 13 |
| Dandelion（%） | 5 | 6 | 4 |
| Barrenwort（%） | 10 | 11 | 8 |
| Radix Isatidis（%） | 13 | 9 | 8 |
| Cordate houttuynia（%） | 8 | 8 | 9 |
| Perilla leaf（%） | 7 | 8 | 9 |
| Asiatic plantain（%） | 6 | 8 | 7 |
| Summation（%） | 100 | 100 | 100 |
Seedling growth test
Take five 25 square metres, 0.6 meter of the depth of water, circular nursery pond（Respectively A groups, B groups, C groups, D groups, E groups）Carry out oyster
Seed rearing is tested, larval rearing density：10 ~ 12/ml of great-hearted oyster larva；Bait：Early stage feeds chrysophyceae（8 ~ 100,000
Cell/ml）, the later stage feeds Chaetoceros（3 ~ 40,000 cells/ml）, flat algae（2 ~ 30,000 cells/ml）Deng；Inflation：Continuous charge, inflation
Amount produces bubble to be advisable on a small quantity with the water surface；Condition of water quality：25 ~ 28 DEG C of water temperature, sea water specific gravity 1.015 ~ 1.017, pH 7.2 ~
7.5；Change water：Change water 1 time within every 2 days, each quantity of exchanged water 30 ~ 40% throws 0.5 ‰ EDTA, cultivation cycle 10 days after changing water.A、B、C
Group adds the Chinese herbal medicine improver of water quality in embodiment 1 ~ 3 respectively, and D groups are changed water and throw penicillin 0.4 ‰ every time, and E groups are without in
Herbal medicine and penicillin, as a comparison case, bait feeding mode is identical with feeding volume.
After cultivation 10 days, oyster seed is determined, survival rate, abnormal rate, average young size, the knot for obtaining are counted respectively
Fruit is as shown in table 2.
The statistics of each embodiment of table 2 and comparative example
From table 2, it is apparent that oyster weed survival rate and housing size are apparently higher than penicillin group and right in each embodiment
Oyster weed survival rate and housing size in ratio, and in each embodiment oyster seed abnormal rate rate significantly lower than penicillin group and
Oyster seed abnormal rate in comparative example, illustrates that Chinese herb feed additive of the invention can remarkably promote the growth of oyster seed, improve seedling
The survival rate planted, and oyster seed abnormal rate can be significantly reduced.
Embodiment described above is a kind of preferably scheme of the invention, not makees any formal to the present invention
Limitation, also has other variants and remodeling on the premise of without departing from the technical scheme described in claim.
Claims (2)
1. it is a kind of improve oyster seed yield fermentation of Chinese herbal medicine additive, it is characterised in that by the quality hundred of following each component
The raw material of point content is made：Gynostemma pentaphylla 6~12%, the coptis 3~5%, sargassum fusifome 4~8%, the % of milk thistle 4 ~ 8, sweet wormwood 5 ~ 10%, thickness
Piao 6 ~ 12%, purslane 10 ~ 15%, dandelion 4 ~ 8%, barrenwort 6 ~ 15%, Radix Isatidis 8 ~ 15%, cordate houttuynia 5 ~ 10%, perilla leaf 5 ~
10% and Asiatic plantain 4 ~ 8%, the percentage by weight sum of each component is 100%.
2. it is a kind of it is as claimed in claim 1 improve oyster seed yield fermentation of Chinese herbal medicine additive preparation and user
Method, it is characterised in that comprise the following steps：
（1）Get the raw materials ready：Impurity in each traditional Chinese medicinal material raw materials is rejected, it is clear with pure water according to above-mentioned each raw material of formula precise
Dried after washing traditional Chinese medicinal material raw materials 3 ~ 4 times, traditional Chinese medicinal material raw materials are crushed to 50~75 mesh, be well mixed, compound Chinese herb is obtained；
（2）Extraction：To the pure water that 30 ~ 40 times are added in compound Chinese herb raw material, in the effect of 300 ~ 600 W ultrasonic assistants
Under, 95~100 DEG C of extraction temperature extracts 30~60 min；
（3）It is prepared by zymotic fluid：To 1.0~3.0% sucrose of addition, 0.5~1.5% peptone, 1.0 in the Chinese herbal medicine feed liquid after extraction
~2.0% peanut meal, 0.2~0.4% K2HPO4, 0.3~0.5% MgSO4.7H2O, 0.02~0.04% CaCl2And 0.02-0.04
% FeSO4.7H2O, pH are adjusted to 7.0~7.5；
（4）Aerobic fermentation：From liquid fermentation tank, liquid amount is 50~70%, 2~4% bacillus subtilises of inoculation, 1~2% ground
Clothing bacillus and 1~3% S. cervisiae, 28~37 DEG C of fermentation temperature of control, the rpm of rotating speed 150~250, throughput 40~
60 L/min, the h of fermentation time 30~40；
（5）Anaerobic fermentation：After aerobic fermentation terminates, 1~3% lactobacillus acidophilus of inoculation, 2~4% Bifidobacteriums and 1~2% nitrification are thin
Bacterium, 28~37 DEG C of fermentation temperature, the rpm of rotating speed 150~250, the h of temperature control anaerobic fermentation 15~25；
（6）Fermentation post processing：After the completion of fermentation, cross 120 ~ 160 mesh filter clothes and obtain ferment filtrate, obtain final product fermentation of Chinese herbal medicine additive；
（7）Usage and dosage：After oyster seedling water changed a water every 2 days, the hair of addition seedling water volume 0.1 ~ 0.4%
Ferment Chinese herb feed additive.
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| CN108740586A (en) * | 2018-06-13 | 2018-11-06 | 威海金牌生物科技股份有限公司 | A kind of oyster fattening bait and preparation method thereof produced with agriculture marine product waste |
| CN109329141A (en) * | 2018-09-28 | 2019-02-15 | 北海冠腾生物科技有限公司 | Method for cultivating raw oyster |
| CN109699810A (en) * | 2018-12-29 | 2019-05-03 | 大连海洋大学 | One seed oyster Chinese herbal medicine solid state fermentation regulation type feed additive |
| CN114794332A (en) * | 2022-05-09 | 2022-07-29 | 广东南洋海洋产业投资有限公司 | Bait formula for enhancing nutrition of oyster fries in larval stage |
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| CN108740586A (en) * | 2018-06-13 | 2018-11-06 | 威海金牌生物科技股份有限公司 | A kind of oyster fattening bait and preparation method thereof produced with agriculture marine product waste |
| CN109329141A (en) * | 2018-09-28 | 2019-02-15 | 北海冠腾生物科技有限公司 | Method for cultivating raw oyster |
| CN109699810A (en) * | 2018-12-29 | 2019-05-03 | 大连海洋大学 | One seed oyster Chinese herbal medicine solid state fermentation regulation type feed additive |
| CN114794332A (en) * | 2022-05-09 | 2022-07-29 | 广东南洋海洋产业投资有限公司 | Bait formula for enhancing nutrition of oyster fries in larval stage |
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