CN114794332A - Bait formula for enhancing nutrition of oyster fries in larval stage - Google Patents
Bait formula for enhancing nutrition of oyster fries in larval stage Download PDFInfo
- Publication number
- CN114794332A CN114794332A CN202210501936.7A CN202210501936A CN114794332A CN 114794332 A CN114794332 A CN 114794332A CN 202210501936 A CN202210501936 A CN 202210501936A CN 114794332 A CN114794332 A CN 114794332A
- Authority
- CN
- China
- Prior art keywords
- parts
- mother liquor
- oyster
- raw materials
- bait
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000237502 Ostreidae Species 0.000 title claims abstract description 51
- 235000020636 oyster Nutrition 0.000 title claims abstract description 51
- 235000016709 nutrition Nutrition 0.000 title claims abstract description 21
- 230000035764 nutrition Effects 0.000 title claims abstract description 20
- 230000001418 larval effect Effects 0.000 title claims abstract description 17
- 230000002708 enhancing effect Effects 0.000 title claims abstract description 15
- 239000012452 mother liquor Substances 0.000 claims abstract description 80
- 239000002994 raw material Substances 0.000 claims abstract description 43
- 239000007788 liquid Substances 0.000 claims abstract description 41
- 239000003814 drug Substances 0.000 claims abstract description 28
- 239000000654 additive Substances 0.000 claims abstract description 24
- 230000000996 additive effect Effects 0.000 claims abstract description 24
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims abstract description 14
- 241000196317 Platymonas Species 0.000 claims abstract description 13
- 241000206751 Chrysophyceae Species 0.000 claims abstract description 12
- 241000209441 Ceratophyllum Species 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 238000000855 fermentation Methods 0.000 claims description 16
- 230000004151 fermentation Effects 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 13
- 238000009395 breeding Methods 0.000 claims description 10
- 230000001488 breeding effect Effects 0.000 claims description 10
- 238000010564 aerobic fermentation Methods 0.000 claims description 8
- 244000063299 Bacillus subtilis Species 0.000 claims description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 4
- 241000186000 Bifidobacterium Species 0.000 claims description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- 235000011305 Capsella bursa pastoris Nutrition 0.000 claims description 4
- 240000008867 Capsella bursa-pastoris Species 0.000 claims description 4
- 241000221079 Euphorbia <genus> Species 0.000 claims description 4
- 240000006509 Gynostemma pentaphyllum Species 0.000 claims description 4
- 235000002956 Gynostemma pentaphyllum Nutrition 0.000 claims description 4
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 4
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 4
- 244000234609 Portulaca oleracea Species 0.000 claims description 4
- 235000001855 Portulaca oleracea Nutrition 0.000 claims description 4
- 241000235342 Saccharomycetes Species 0.000 claims description 4
- 241000264279 Sargassum fusiforme Species 0.000 claims description 4
- 244000272459 Silybum marianum Species 0.000 claims description 4
- 235000010841 Silybum marianum Nutrition 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 235000011148 calcium chloride Nutrition 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- 238000011049 filling Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 239000008213 purified water Substances 0.000 claims description 4
- 229960004793 sucrose Drugs 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- 241000941774 Lysimachia terrestris Species 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims 5
- 241000227752 Chaetoceros Species 0.000 claims 1
- 240000001949 Taraxacum officinale Species 0.000 claims 1
- 241000195493 Cryptophyta Species 0.000 abstract description 4
- 230000018109 developmental process Effects 0.000 abstract description 4
- 230000002180 anti-stress Effects 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 3
- 230000029052 metamorphosis Effects 0.000 abstract description 3
- 238000005728 strengthening Methods 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 230000003115 biocidal effect Effects 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 230000006872 improvement Effects 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 241000722731 Carex Species 0.000 abstract 1
- 241000218554 Lyophyllum Species 0.000 abstract 1
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 241000245665 Taraxacum Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 241001098608 Ceratophyllus Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000036772 blood pressure Effects 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 241000237519 Bivalvia Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000726768 Carpinus Species 0.000 description 1
- 201000000297 Erysipelas Diseases 0.000 description 1
- 241000422392 Laurencia Species 0.000 description 1
- 241000237852 Mollusca Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000490567 Pinctada Species 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000007653 larval development Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Inorganic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Botany (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a bait formula for enhancing nutrition of oyster fries in a larval stage, which comprises the following raw materials in parts by weight: 25-36 parts of chlorella mother liquor, 7-8 parts of diatom mother liquor, 5-7 parts of Platymonas vernalis mother liquor, 3-7 parts of chrysophyceae mother liquor, 2-5 parts of Lyophyllum terrestris mother liquor and 1-2 parts of Ceratophyllum purpureum mother liquor, wherein the chlorella mother liquor, the diatom mother liquor, the Platymonas vernalis mother liquor and the Chrysophytum indicum mother liquor areThe concentration of algae mother liquor, Larrenba mother liquor and Carex mother liquor is 1.2 × 10 8 ～1.8×10 8 cells/ml. The bait prepared by the invention can improve the requirements of larva on nutrition strengthening and metamorphosis survival rate improvement, improve the immunity and anti-stress capability and accelerate the development of the larva; the liquid medicine additive can replace antibiotic medicine, effectively inhibit disease microorganisms and optimize the living environment of oyster larva.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to a bait formula for nutrition enrichment of oyster fries in a larval stage.
Background
The oyster belongs to the phylum mollusca, class bivalvia, order pearl oyster, family oyster, widely distributed in all sea areas of the world, and is one of the important marine fishery resources in all parts of the world. The meat is fat and smooth, tastes delicious and is rich in nutrition, contains rich nutritional ingredients such as protein, fat, calcium, phosphorus, iron and the like, and is called as seabed milk. The oyster also has good food therapy effect. The traditional Chinese medicine considers that the oyster is sweet and nontoxic, can relieve five internal organs, regulate middle-jiao, tonify qi and nourish blood to relieve erysipelas, sober up, quench thirst, activate blood and allay hunger, and has the functions of moistening skin, beautifying and nourishing skin after being eaten for a long time. Modern medicine also considers that oyster meat also has the functions of lowering blood pressure, nourishing yin and blood and the like. The oyster also has various functions of 'fine skin, beautifying face', reducing blood pressure, nourishing yin and blood, building body and strengthening body, and the like, so the oyster is regarded as delicious seafood and body building food. Oysters are important mariculture objects in all countries of the world and become economic shellfish with the largest culture yield in China and even the world. The consumption demand is continuously enlarged, the rapid development of the oyster breeding industry is promoted, the seedling demand is increased, and the artificial seedling culture is rapidly developed. However, in the oyster seedling raising production, the problems of low hatchability, disintegration and death of the faceplates and the like frequently occur, and the yield of oyster seedlings is influenced. Therefore, a bait formula for enhancing nutrition of oyster fries in the larval stage is needed to improve the requirement of larval metamorphosis survival rate, improve immunity and anti-stress capability and accelerate larval development.
Disclosure of Invention
The invention aims to provide a bait formula for enhancing nutrition of oyster fries in the larval stage, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a bait formula for enhancing nutrition of oyster fries in a larval stage is composed of the following raw materials in parts by weight: 25-36 parts of chlorella mother liquor, 7-8 parts of diatom mother liquor, 5-7 parts of Platymonas vernalis mother liquor, 3-7 parts of chrysophyceae mother liquor, 2-5 parts of Lysimachia terrestris mother liquor and 1-2 parts of ceratophyllum mother liquor.
Preferably, the concentration of the chlorella mother liquor, diatom mother liquor, Platymonas mother liquor, Chrysophyta mother liquor, Langleaf mother liquor and Ceratophyllum mother liquor is 1.2 × 10 8 ～1.8×10 8 cells/ml。
Preferably, the density of the oyster larva in the breeding water containing the oyster larva is 60-70 per ml of water.
Preferably, the bait formula also comprises a liquid medicine additive, and the weight part of the liquid medicine additive in the bait formula is 10-15 parts.
Preferably, the liquid medicine additive is prepared from the following raw materials in parts by weight: 7-10 parts of gynostemma pentaphylla, 3.5-4.5 parts of humifuse euphorbia herb, 5-7 parts of sargassum fusiforme, 5-7 parts of silybum marianum, 6-9 parts of shepherd's purse, 7-11 parts of mangnolia officinalis, 11-14 parts of purslane and 5-8 parts of dandelion.
Preferably, the preparation method of the liquid medicine additive specifically comprises the following steps:
(1) preparing raw materials according to the raw material proportion of the liquid medicine additive, cleaning the raw materials, fully drying the raw materials at 30-40 ℃, crushing the raw materials by a crusher, and mixing the raw materials to obtain a raw material mixture;
(2) putting the raw material mixture into an extraction container, adding purified water of 40-50 times, boiling at normal pressure, preserving heat for 50 minutes, and stirring in the heat preservation process;
(3) cooling the liquid obtained in the step (2) to normal temperature, and adding 1-2 parts by weight of cane sugar, 0.5-1 part by weight of albumen powder and 0.2-0.3 part by weight of K 2 HPO 4 0.2 to 0.4 part of MgSO 4 7H2O and 0.02-0.04 part of CaCl2, and adjusting the pH value to 7.1-7.4;
(4) selecting a liquid fermentation tank, wherein the liquid filling amount is 60%, inoculating bacillus subtilis and saccharomycetes, controlling the fermentation temperature to be 30-35 ℃, stirring at the stirring speed of 200rpm, the ventilation volume of 50L/min, and the aerobic fermentation time of 55 h;
(5) after aerobic fermentation, inoculating lactobacillus acidophilus and bifidobacterium, controlling the fermentation temperature to be 28-33 ℃, and stirring at the stirring speed of 200rpm for 20 hours;
(6) filtering after fermentation to obtain the liquid medicine additive.
The invention has the technical effects and advantages that: the bait formula disclosed by the invention is comprehensive in nutrition, easy to digest, good in palatability and excellent in food calling and ingestion effect, so that the growth and development and metabolism of oyster fries are accelerated; the bait can improve the requirements of nutrition strengthening and metamorphosis survival rate improvement of the larvae, improve the immunity and anti-stress capability and accelerate the development of the larvae; the liquid medicine additive can replace antibiotic medicine, effectively inhibit disease microorganisms and optimize the living environment of oyster larva.
Detailed Description
Example 1
A bait formula for enhancing nutrition of oyster fries in a larval stage is composed of the following raw materials in parts by weight: 25 parts of chlorella mother liquor, 7 parts of diatom mother liquor, 5 parts of Platymonas vernalis mother liquor, 3 parts of chrysophyceae mother liquor, 2 parts of Larrella terrestris mother liquor, 1 part of Ceratophyllus terrestris mother liquor, and the concentrations of the chlorella mother liquor, the diatom mother liquor, the Platymonas vernalis mother liquor, the chrysophyceae mother liquor, the Halbaculoides mother liquor and the Ceratophyllus terrestris mother liquor are 1.2 multiplied by 10 8 cells/ml; the density of the larvae of the oyster fries in the culture water containing the larvae of the oyster fries is 60/ml water. When the bait for enhancing the nutrition of the oyster larva larvae is used, the weight ratio of the bait to the breeding water containing the oyster larva is 1: 1000.
Example 2
A bait formula for enhancing nutrition of oyster fries in a larval stage is composed of the following raw materials in parts by weight: 36 parts of chlorella mother liquor, 8 parts of diatom mother liquor, 7 parts of Platymonas vernalis mother liquor, 7 parts of chrysophyceae mother liquor, 5 parts of Laurencia terrestris mother liquor, 2 parts of Ceratophyllum aculeatus mother liquor, the concentrations of the chlorella mother liquor, the diatom mother liquor, the Platymonas vernalis mother liquor, the chrysophyceae mother liquor, the Haliobacter terrestris mother liquor and the Ceratophyllum aculeatus mother liquor are 1.8 multiplied by 10 8 cells/ml; the density of the larvae of the oyster fries in the breeding water containing the larvae of the oyster fries is 70/ml water. When the bait for enhancing the nutrition of the oyster larva larvae is used, the weight ratio of the bait to the breeding water containing the oyster larva is 1: 900.
Example 3
A bait formula for enhancing nutrition of oyster fries in a larval stage is composed of the following raw materials in parts by weight: 25 parts of chlorella mother liquor, 7 parts of diatom mother liquor, 5 parts of Platymonas vernalis mother liquor, 3 parts of chrysophyceae mother liquor, 2 parts of Larrella bracteata mother liquor, 1 part of Ceratophyllum purpureum mother liquor, 10 parts of liquid medicine additive, chlorella mother liquor and diatom mother liquorThe concentration of the liquid, mother liquid of Platymonas, mother liquid of Chrysophyta, mother liquid of Lorentiba and mother liquid of Ceratophyllum is 1.2 × 10 8 cells/ml; the density of the larvae of the oyster fries in the breeding water containing the larvae of the oyster fries is 60/ml water. When the bait for enhancing the nutrition of the oyster larva larvae is used, the weight ratio of the bait to the breeding water containing the oyster larva is 1: 1000.
The liquid medicine additive is prepared from the following raw materials in parts by weight: 7 parts of gynostemma pentaphylla, 3.5 parts of humifuse euphorbia herb, 5 parts of sargassum fusiforme, 5 parts of silybum marianum, 6 parts of shepherd's purse, 7 parts of mangnolia officinalis, 11 parts of purslane and 5 parts of dandelion; the preparation method of the liquid medicine additive specifically comprises the following steps:
(1) preparing raw materials according to the raw material proportion of the liquid medicine additive, cleaning the raw materials, fully drying the raw materials at 30-40 ℃, crushing the raw materials by a crusher, and mixing the raw materials to obtain a raw material mixture;
(2) putting the raw material mixture into an extraction container, adding 40 times of purified water, boiling under normal pressure, preserving heat for 50 minutes, and stirring in the heat preservation process;
(3) cooling the liquid obtained in the step (2) to normal temperature, and adding 1 part of cane sugar, 0.5 part of albumen powder and 0.2 part of K in parts by weight 2 HPO 4 0.2 part of MgSO 4 7H2O and 0.02 part of CaCl2, and adjusting the pH value to 7.1-7.4;
(4) selecting a liquid fermentation tank, wherein the liquid filling amount is 60%, inoculating bacillus subtilis and saccharomycetes, controlling the fermentation temperature to be 30-35 ℃, stirring at the stirring speed of 200rpm, the ventilation volume of 50L/min, and the aerobic fermentation time of 55 h;
(5) after aerobic fermentation, inoculating lactobacillus acidophilus and bifidobacterium, controlling the fermentation temperature to be 28-33 ℃, and stirring at the stirring speed of 200rpm for 20 hours;
(6) filtering after fermentation to obtain the liquid medicine additive.
Example 4
A bait formula for enhancing nutrition of oyster fries in a larval stage is composed of the following raw materials in parts by weight: 36 parts of chlorella mother liquor, 8 parts of diatom mother liquor, 7 parts of Platymonas vernalis mother liquor, 7 parts of chrysophyceae mother liquor, 5 parts of Larrella bracteata mother liquor and cornua cervicalensis2 parts of thorn algae mother liquor, 15 parts of liquid medicine additive, and the concentration of chlorella mother liquor, diatom mother liquor, flat algae mother liquor, gold algae mother liquor, Langerbaf mother liquor and hornbeam mother liquor is 1.8 multiplied by 10 8 cells/ml; the density of the larvae of the oyster fries in the breeding water containing the larvae of the oyster fries is 70/ml water. When the bait for enhancing the nutrition of the oyster larva larvae is used, the weight ratio of the bait to the breeding water containing the oyster larva is 1: 900.
The liquid medicine additive is prepared from the following raw materials in parts by weight: 10 parts of gynostemma pentaphylla, 4.5 parts of humifuse euphorbia herb, 7 parts of sargassum fusiforme, 7 parts of silybum marianum, 9 parts of shepherd's purse, 11 parts of mangnolia officinalis, 14 parts of purslane and 8 parts of dandelion; the preparation method of the liquid medicine additive specifically comprises the following steps:
(1) preparing raw materials according to the raw material proportion of the liquid medicine additive, cleaning the raw materials, fully drying the raw materials at 30-40 ℃, crushing the raw materials by a crusher, and mixing the raw materials to obtain a raw material mixture;
(2) putting the raw material mixture into an extraction container, adding 50 times of purified water, boiling under normal pressure, preserving heat for 50 minutes, and stirring in the heat preservation process;
(3) cooling the liquid obtained in the step (2) to normal temperature, and adding 2 parts by weight of cane sugar, 1 part by weight of albumen powder and 0.3 part by weight of K 2 HPO 4 0.4 part of MgSO 4 7H2O and 0.04 part of CaCl2, and adjusting the pH value to 7.1-7.4;
(4) selecting a liquid fermentation tank, wherein the liquid filling amount is 60%, inoculating bacillus subtilis and saccharomycetes, controlling the fermentation temperature to be 30-35 ℃, stirring at the stirring speed of 200rpm, the ventilation volume of 50L/min, and the aerobic fermentation time of 55 h;
(5) after aerobic fermentation, inoculating lactobacillus acidophilus and bifidobacterium, controlling the fermentation temperature to be 28-33 ℃, and stirring at the stirring speed of 200rpm for 20 hours;
(6) filtering after fermentation to obtain the liquid medicine additive.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (6)
1. A bait formula for enhancing nutrition of oyster fries in a larval stage is characterized by comprising the following raw materials in parts by weight: 25-36 parts of chlorella mother liquor, 7-8 parts of diatom mother liquor, 5-7 parts of Platymonas vernalis mother liquor, 3-7 parts of chrysophyceae mother liquor, 2-5 parts of Lysimachia terrestris mother liquor and 1-2 parts of ceratophyllum mother liquor.
2. A bait formulation for larval-stage fortification of oyster offspring according to claim 1, characterized in that: the concentration of the chlorella mother liquor, the diatom mother liquor, the Platymonas mother liquor, the chrysophyceae mother liquor, the Langerbavin mother liquor and the Chaetoceros mother liquor is 1.2 multiplied by 10 8 ～1.8×10 8 cells/ml。
3. A bait formulation for larval stage fortification of oyster fries according to claim 1, characterized in that: the density of the larvae of the oyster fries in the breeding water containing the larvae of the oyster fries is 60-70/ml water.
4. A bait formulation for larval stage fortification of oyster fries according to claim 1, characterized in that: the bait formula also comprises a liquid medicine additive, and the weight part of the liquid medicine additive in the bait formula is 10-15 parts.
5. A bait formulation for larval stage fortification of oyster fries according to claim 4, wherein: the liquid medicine additive is prepared from the following raw materials in parts by weight: 7-10 parts of gynostemma pentaphylla, 3.5-4.5 parts of humifuse euphorbia herb, 5-7 parts of sargassum fusiforme, 5-7 parts of silybum marianum, 6-9 parts of shepherd's purse, 7-11 parts of mangnolia officinalis, 11-14 parts of purslane and 5-8 parts of dandelion.
6. A bait formulation for the fortification of oyster offspring at the larval stage according to claim 5 wherein: the preparation method of the liquid medicine additive specifically comprises the following steps:
(1) preparing raw materials according to the raw material proportion of the liquid medicine additive, cleaning the raw materials, fully drying the raw materials at 30-40 ℃, crushing the raw materials by a crusher, and mixing the raw materials to obtain a raw material mixture;
(2) putting the raw material mixture into an extraction container, adding purified water of 40-50 times, boiling at normal pressure, preserving heat for 50 minutes, and stirring in the heat preservation process;
(3) cooling the liquid obtained in the step (2) to normal temperature, and adding 1-2 parts by weight of cane sugar, 0.5-1 part by weight of albumen powder and 0.2-0.3 part by weight of K 2 HPO 4 0.2 to 0.4 part of MgSO 4 7H2O and 0.02-0.04 part of CaCl2, and adjusting the pH value to 7.1-7.4;
(4) selecting a liquid fermentation tank, wherein the liquid filling amount is 60%, inoculating bacillus subtilis and saccharomycetes, controlling the fermentation temperature to be 30-35 ℃, stirring at the stirring speed of 200rpm, the ventilation volume of 50L/min, and the aerobic fermentation time of 55 h;
(5) after aerobic fermentation, inoculating lactobacillus acidophilus and bifidobacterium, controlling the fermentation temperature to be 28-33 ℃, and stirring at the stirring speed of 200rpm for 20 hours;
(6) filtering after fermentation to obtain the liquid medicine additive.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210501936.7A CN114794332A (en) | 2022-05-09 | 2022-05-09 | Bait formula for enhancing nutrition of oyster fries in larval stage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210501936.7A CN114794332A (en) | 2022-05-09 | 2022-05-09 | Bait formula for enhancing nutrition of oyster fries in larval stage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114794332A true CN114794332A (en) | 2022-07-29 |
Family
ID=82513627
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210501936.7A Pending CN114794332A (en) | 2022-05-09 | 2022-05-09 | Bait formula for enhancing nutrition of oyster fries in larval stage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114794332A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598477A (en) * | 2013-11-13 | 2014-02-26 | 浙江海洋学院 | Base bait formula for mytilus crassitesta planktonic larva |
| CN104799082A (en) * | 2015-04-20 | 2015-07-29 | 江苏科技大学 | Microalgae composite living bait for shellfish breeding and production method of microalgae composite living bait |
| CN106819655A (en) * | 2017-02-17 | 2017-06-13 | 浙江万里学院 | A kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield and its preparation application method |
| CN109090380A (en) * | 2018-09-12 | 2018-12-28 | 浙江海洋大学 | A kind of bait formula for axe clam floating larvae phase fortification |
-
2022
- 2022-05-09 CN CN202210501936.7A patent/CN114794332A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103598477A (en) * | 2013-11-13 | 2014-02-26 | 浙江海洋学院 | Base bait formula for mytilus crassitesta planktonic larva |
| CN104799082A (en) * | 2015-04-20 | 2015-07-29 | 江苏科技大学 | Microalgae composite living bait for shellfish breeding and production method of microalgae composite living bait |
| CN106819655A (en) * | 2017-02-17 | 2017-06-13 | 浙江万里学院 | A kind of fermentation of Chinese herbal medicine additive for improving oyster seed yield and its preparation application method |
| CN109090380A (en) * | 2018-09-12 | 2018-12-28 | 浙江海洋大学 | A kind of bait formula for axe clam floating larvae phase fortification |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR20060125455A (en) | Cultivation for feeding a seas lug and method of manufacturing the same | |
| CN105557580B (en) | A kind of cultural method of Macrobrachium Rosenbergii Juvenile | |
| CN106135664A (en) | A kind of for microbial ecological agent treating and preventing disease of alimentary tract of livestock husbandry and birds and its production and use | |
| CN108783106A (en) | A kind of bionical feed of mandarin fish adult fish culture and preparation method thereof | |
| CN108813126A (en) | A kind of pseudosciaena crocea larvae composite immune reinforcing agent and its preparation method and application | |
| CN107549309B (en) | Fruity selenium-rich cordyceps militaris yoghourt product and preparation method thereof | |
| CN106577379A (en) | Culture method of selenium-rich weevers | |
| CN102987163A (en) | Immune-enhancing feed and preparation method thereof | |
| CN106615973A (en) | Feed for laying ducks and preparation method thereof | |
| CN106234302A (en) | A kind of cultural method of layer of green-shell egg | |
| CN107494362A (en) | A kind of bait intensifying method for improving hickie squid larva survival rate | |
| CN107307407A (en) | A kind of fruit ferment drink | |
| CN112293615A (en) | Overwintering feed for golden pomfret and preparation and culture method thereof | |
| CN101919360A (en) | Turbot fry breeding method | |
| CN114467812B (en) | Tilapia embrittlement breeding method | |
| CN114794332A (en) | Bait formula for enhancing nutrition of oyster fries in larval stage | |
| CN110583362A (en) | Black termitomyces albuminosus cultivation method | |
| KR20170079910A (en) | Feed additives for abalone | |
| CN101301315B (en) | Uses of black-tail snake in preparing bait for treating lymphocyst of flatfish | |
| CN114223809A (en) | Fermented feed for crabs as well as preparation method and application of fermented feed | |
| CN114617088A (en) | Breeding method for improving immunity of Babylonia | |
| CN106578582A (en) | Feed for young ostriches and preparation method of feed | |
| CN112655621A (en) | River crab culture method for improving acre yield | |
| CN108740586A (en) | A kind of oyster fattening bait and preparation method thereof produced with agriculture marine product waste | |
| CN108850581A (en) | A kind of feed and its processing method for ecologic breeding milk cow |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220729 |