KR20170079910A - Feed additives for abalone - Google Patents

Feed additives for abalone Download PDF

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Publication number
KR20170079910A
KR20170079910A KR1020150190957A KR20150190957A KR20170079910A KR 20170079910 A KR20170079910 A KR 20170079910A KR 1020150190957 A KR1020150190957 A KR 1020150190957A KR 20150190957 A KR20150190957 A KR 20150190957A KR 20170079910 A KR20170079910 A KR 20170079910A
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KR
South Korea
Prior art keywords
extract
abalone
feed
fermented
growth
Prior art date
Application number
KR1020150190957A
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Korean (ko)
Inventor
김영남
Original Assignee
김영남
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Priority to KR1020150190957A priority Critical patent/KR20170079910A/en
Publication of KR20170079910A publication Critical patent/KR20170079910A/en

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Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

Abstract

The present invention is a new alternative protein source for replacing costly fish meal for abalone production. It is an eco-friendly abalone substitute feed additive using mung bean as a marine waste. It replaces Spirulina fish meal and wheat meal as mushrooms, As a composition for growth promotion having effects of improving feed efficiency, improving feed efficiency, activating digestive enzyme secretion, increasing immunity, improving reproductive productivity, antimicrobial activity, and antioxidation as well as supplementing insufficient nutrients in feed, , Which not only promotes the growth of abalone, but also increases the resistance to changes in water quality and disease, thereby promoting the growth of abalone and improving the growth rate. .

Description

{Feed additives for abalone} containing an extract of fermented mung bean and fermented plant complex as an active ingredient,

The present invention relates to an abalone feed additive comprising fermented mash mash extract as an active ingredient,

As a new alternative protein source for replacing expensive fish meal for abalone production, it is an eco-friendly abalone substitute feed additive using mung bean that is abandoned as a marine waste. By replacing the spirulina fish meal and wheat flour with each other, As a composition for growth promotion having effects of improving feed efficiency, improving feed efficiency, activating digestive enzyme secretion, increasing immunity, improving reproductive productivity, antimicrobial activity, and antioxidation as well as supplementing insufficient nutrients in feed, , Which not only promotes the growth of abalone, but also increases the resistance to changes in water quality and disease, thereby promoting the growth of abalone and improving the growth rate. .

Abalone is a high-value food cultivated from ancient times as a high-priced preference food with industrial prospects. Abalone's body is traditionally high-quality fish food and inner shell surface with iridescent pearl luster is used as a craft for high-grade mother-of-pearl. And abalone farming is also used as a seed for pearl farming.

Recently, abalone farming industry in Korea has grown greatly, but the disease of abalone during farming has been a crucial factor for hindering development of abalone farming industry.

In recent years, abalone farming has mainly been carried out by artificial nursery production, in which the seedlings are discharged to the reef area and re-harvested in the size of the commodity. The abalone is cultivated in the aquaculture tank as a compound feed to improve the survival rate and growth rate of abalone. The most important consideration in the aquaculture is proper water temperature and sufficient food supply, and it is possible to maintain the proper water temperature of abalone at 18 ~ 22 ℃ in aquaculture tank style. Has become an important factor.

Stable supply of feed is becoming a big obstacle in abalone farming. In other words, abalone cultured fish are mainly used for nourishing food such as seaweed and raw seaweed, and they supply dried seaweed, kelp, and salted seaweed in the summer season.

In the abalone diet of natural feed, feed is the main factor that determines the success of the aquaculture. It not only grows abalone but also affects water pollution and economic efficiency. When nourishing food is used for natural food such as camphor and raw kelp It is reported that the growth effect is lower than that of the compound feed because the production supply is unstable due to the timing and local circumstances and the price fluctuation is severe and the main nutrient content is lower than the required amount for normal growth of the abalone.

Especially, fishermen who cultivate abalone are using natural food such as seaweed, parasites, and kelp as food for breeding, and they supply dried seaweeds and dried kelp in summer, which is a limiting factor for systematic aquaculture.

Currently, abalone is dependent on seaweed and kelp. It needs about 600,000 tons of seaweed for the production of 30,000 tons of abalone, but the production of algae is not much bigger than that for abalone production.

In other words, the supply of natural food is not only unstable but also has a disadvantage of fluctuating price and it is also reported that the growth rate is lower than that of mixed nutrients which have various nutrients required for aquaculture. Therefore, it is urgently required to supply alternative feeds for abalone cultivation which can produce more economically and at the same time, growth of abalone is advantageous

Seaweeds are classified into red algae, green algae and brown algae according to their color, photosynthetic pigment. Brown algae include seaweed, kelp, tortoise, mackerel, tortoiseshell, crab, and seal. Red algae refers to such things as Kim, mugwort, and squid. The green algae have something like flower buds, parasites, and hearing.

In addition, the good pharmacological effects of seaweeds have been clarified and the interest of modern people in seaweed has been increasing. The Korean moth is distributed in Busan, Mokdo, Geoje Island, Namhae Island, Jeju Island and Chuza Island. It has excellent cell protection effect. It is known that the cell protection effect is better than the resveratrol wine or the catechin green tea component.

In particular, 'Hornseye mackerel' is a non-profitable seaweed that has been infested by shrimp farms in the areas of Shinan and Haenam, and has suffered a great deal of damage. The mackerel is caught in a farm net. When the water temperature rises, it rapidly grows to prevent growth of seaweed and sea tangle. It is harmful to the style such as abalone because we stop communication of farm bird.

Accordingly, the present inventor has developed various additives for the abalone feed containing the fermented mash mash extract as an effective ingredient in order to maximize the efficiency of the feed, although the nutrients are abundant.

The inventors of the present invention have developed an abalone feed additive containing an extract of fermented mung bean as an active ingredient as described above, thereby improving the adaptability to changes in the breeding environment of abalone, growing abalone healthy without infecting diseases during overturning period, The feed efficiency can be maximized through an increase in the feed intake rate, thereby reducing the economic loss due to overturning of the abalone, and also achieving the environment-friendly manner by reducing the pollution of the marine environment. will be.

As described above, the conventional overturned feed has various problems,

As an alternative protein source that can replace fish meal, it is an eco-friendly abalone substitute feed additive using fermented mash mushroom extract that is turned into marine waste. It replaces spirulina fish meal and wheat flour each with mushroom, supplementing insufficient nutrients in conventional overturning compound feed As well as a composition for growth promotion having effects of improving feed efficiency, increasing amount of fat, activating digestive enzyme secretion, increasing immunity, improving reproductive productivity, antimicrobial activity and antioxidation, It is an object of the present invention to solve the technical problem of developing economical high-quality overturned feed additive which is able to promote the growth of the abalone and improve the growth rate by increasing the resistance to changes in water quality and diseases.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems of the prior art,

An overturned feed additive comprising seaweeds,

An abalone feed additive containing fermented mash mushroom extract as an active ingredient,

After immersion and washing, the vacuum-dried moth is finely pulverized, and then the fermenting microorganism bacillus subtilis (Bacillus subtilis) is used to perform the action of the fermenting microorganism for 20 to 30 hours at a temperature of 20 ° C to 40 ° C Fermented mungbean extracts obtained by fermentation at low temperature and fermentation, and extracts of plant extracts containing natural herb medicine, bokbunja extract, white mulberry extract, early stage extract and fermented extract of Saururus chinensis extract as effective ingredients in one or more equivalent ratios are mixed at a total weight ratio of 10: 1 < / RTI > as an active ingredient.

As described above,

The present invention relates to an abalone feed additive containing an effective fermented mash mushroom extract which can replace an antibiotic, as well as nutritionally balanced nutritional and vitamin inorganic substances in the conventional abalone feed, Which is effective for improving growth, promoting digestion, enhancing digestive enzymes, promoting digestive enzymes secretion, increasing immunity, improving reproductive productivity, antimicrobial activity, antioxidation, etc. , The growth of abalone can be promoted and a healthy abalone can be cultivated, thereby maximizing the efficiency of the feed

The present invention can be used as an eco-friendly abalone feed additive using mung bean as an economical additive for marine algae as a seaweed, and it is possible to replace the spirulina meal and wheat meal with mung bean, thereby reducing the cost for protein source of abalone feed. .

That is, the present invention can meet the nutritional requirement of the abalone required for rapid growth by adding fermented mackerel as an algae extract and replacing an animal protein with a plant protein, thereby enhancing growth and affecting body composition, To improve the adaptability to the environmental change of the rollover, or to increase the physiological activity of the active ingredient on the rollover, thereby improving the feed efficiency efficiently.

Particularly, the feed additive of the present invention includes a fermented product obtained by fermenting a fermented Bacillus subtilis strain having an alginic acid decomposing ability and an antibacterial activity in a feed composition containing a mothball, so that when the feed is fed to the abalone, The bioavailability is increased and the amount of body weight of the abalone is increased and the antimicrobial substance is included, so that the immunity against the pathogenic bacteria of the fish is increased and the abortion growth rate and the survival rate can be improved

In order to achieve the above object, the present invention will be described in detail.

In the overturned feed additive comprising the seaweed of the present invention,

The abalone feed additive containing fermented mash mash extract as an active ingredient is prepared by immersing and washing, then finely pulverizing the vacuum dried mushroom, and then using a fermenting microorganism bacillus subtilis (Bacillus subtilis) And fermented extract of fermented mung bean extract, which is fermented and extracted by fermentation at low temperature for 20 to 30 hours under the action of fermentation microorganism, Which comprises a fermented mash mushroom extract obtained by mixing a plant complex extract containing an active ingredient at a total weight ratio of 10: 1, as an active ingredient.

The composition of the present invention, which has been mixed and compounded as described above, is preferably added as an essential ingredient to be normally added as an abalone feed additive considering the physiology of abalone, and it is preferable to add 5 to 25% by weight in the total weight ratio in the abalone- Functional food additive composition is added in an appropriate amount in accordance with the overturning growth step in a compounded diet comprising protein, carbohydrate, lipid and fatty acid, minerals as nutrients, and various vitamins, Increase digestive power and immunity, and form an environmentally friendly compound feed close to the most natural food with optimal taste and nutrients. Underwater corruption at high temperature is less, preventing feed loss and water pollution, enabling eco-friendly form of healthy abalone. do.

When the feed additive of the present invention is added to the overturned combination feed, if the content of the feed additive is too small, the effect of addition is small and if the added amount is too large, it becomes uneconomical and not preferable. Most preferred is the addition of the functional feed additive of the present invention By weight based on the total weight of the composition.

The present invention ferments mung bean as brown algae with bacillus subtilis (Bacillus subtilis) as a fermenting microorganism, and obtains effective fucosanthin in high yield. Such microorganisms are exemplified for illustrative purposes only and various microorganisms And microorganisms may be obtained in a natural state, commercially available ones, or may be used from microorganism depository institutions, preferably Bacillus subtilis having a good protein degrading ability. desirable.

In the present invention, the molecular weight of the fuco xanthine is preferably 2 moles by microbial fermentation because the bimolecular structure is very large and the bacterium is not easily absorbed unless the bacterium is subdivided into 10,000 or less.

The present invention can obtain an extract at a high yield by a relatively simple procedure while minimizing the loss of fucoxanthin as an active ingredient from a brown louse as a brown algae.

Fucoidan, a physiologically active substance of brown algae, is known to induce apoptosis leading to the death of cancer cells, and to inhibit the growth of cancer cells. Inhibition ▲ Cancer cells to prevent cancer metastasis of platelet adhesion inhibition ▲ Our body's defense system to attack cancer, such as immunity enhancement is excellent, and has become a natural anti-cancer drug.

Also, the natural herbal medicine which is included in the feed additive of the present invention as an active ingredient and produces safe abalone by replacing the antibiotic, is a natural herb medicine which comprises five kinds of natural herbal medicine extracts of bamboo extract, bokbunja extract, The content of the plant complex extract is not limited, but may be included in the same amount as the total weight of the plant complex extract composition. The extracts of the plant extract, bokbunja extract, white sachet extract, %.

As used herein, the term 'extract' has the meaning of being used as a crude extract in the art, and the composition ratio of each ingredient can be added or subtracted within a usual range when the feed additive of the present invention is manufactured.

In the present invention, the plant complex extract of the present invention is a composition for promoting abalone growth comprising at least one of natural extract, crude extract, bamboo extract, early extract, saururus chinensis extract as an active ingredient, The present invention provides a method for improving the survival rate and the growth rate of abalone to improve the ability to infect and improve healthy abalone. In particular, in the present invention, among a plurality of plant- ) It is composed of a herb medicine which increases the production amount and is formed at an optimum compounding ratio. It stimulates bioprotein synthesis and cell differentiation by IGF (growth factor) promoting effect and promotes the growth of abalone and promotes immune function

The herbal medicine used in the present invention is widely used as a medicament since the medicinal effect is described in detail in the medicinal herb medicine.

Glucoside and saponin are the main ingredients of astragalus, and they are used as expectorants for bronchitis, and are effective for anemia, insomnia,

Bokbunja is a medicinal herb of wild raspberries belonging to Rosaceae. It has antioxidant, anticholesterol, liver and gastrointestinal function improvement. Its efficacy is mentioned in various documents such as Dongbibogam, In pharmacological analysis of medicine, it has been found that polyphenol is contained in a large amount in the fruit, anti-cancer effect, anti-aging, prevention of arteriosclerosis, prevention of thrombosis,

Cynanchum wilfordii is a perennial plant belonging to the family Polygonaceae, having a height of 1-3 m, and its roots are thick roots. The main ingredients of white seaweed are cinanotoxin, phytotoxotoxin, etc., and it is used as a tonic and anti-

Phlomis umbrosa is a 1 m high perennial herb belonging to the Lamiaceae family, with a height of 70-150 ㎝ and a height of about 5 root. Leaves are used for food, roots and rootstocks are used for medicinal purposes. It is known to have pharmacological effects to protect the liver and kidneys. It contains alkaloids, essential oils and vitamins.

Therefore, when the compound feed additive of the present invention manufactured as described above is mixed with the general compound feed at a proper ratio and fed to the abalone, realization of environmentally friendly abalone breeding will be performed to strengthen the immunity and to prevent and treat various diseases, And the growth rate can be improved, so that the mortality rate can be reduced and the feed efficiency can be increased.

Production of functional feed additives

1-1. Preparation of fermented mung bean extract

Dried bacon is finely pulverized and then fermented at a temperature of 20 ° C to 40 ° C using bacillus subtilis (Bacillus subtilis) as a fermenting microorganism for 20 to 30 hours at low temperature under the action of a fermenting microorganism. An extract was obtained.

1-2. Manufacture of plant complex extracts

IGF (growth factor) was synthesized by mixing raw extracts of native oriental medicine, bokbunja extract, white mint extract, early stage extract and extract of Saururus chinensis extract in a composition ratio (weight ratio, equivalent ratio) of 1: 1: A composite extract of a fermented plant of the present invention was prepared as a composition for promoting abalone growth which increased the production amount.

1-3. Preparation of Functional Feed Additives including Extract of Fermented Mushrooms and Fermented Plant Combined Extracts

1-1. Fermented mash mushroom extract of the present invention and the plant complex extracts obtained from the 1-2 mushroom process were mixed at a ratio of 10: 1 in total weight ratio to prepare an abalone feed additive containing the fermented mash mash extract as an active ingredient.

Manufacture of abalone formula containing functional food additive

The abalone feed additive obtained in the above step 1 was added to the prepared feed mixture in an amount of 15 wt%: 85 wt% based on the total weight ratio, and then mixed with stirring to prepare an abalone mixed feed supplemented with the functional feed additive of the present invention

Test example:

The commercial off-the-blend diets supplemented with the functional feed additive of the present invention produced in Example 2 were prepared as feedstuffs for four weeks without any addition of the functional additive of the present invention, and the feed intake rate, growth rate, In addition, the feed efficiency of the present invention was improved compared with the feed without the functional additive of the present invention, and the feed efficiency and the feed efficiency were significantly improved. Thus, it was found that the abalone compound feed added with the present invention realizes remarkable intake and digestibility and improved survival rate.

As a result, the use of the present invention ultimately provides a high-quality feed additive for the whole body, thereby promoting the growth of abalone and reducing the loss of feed due to an increase in the feed intake rate, thereby improving the productivity by reducing the feed cost, By solving the marine pollution problem, it was found that the economical and environmental friendly form of healthy abalone could be made possible.

Therefore, the overturned feed additive of the present invention significantly reduces the stress of the abalone, and the appetite becomes strong, and the growth rate and the disease prevention rate are remarkably improved, so that it is necessary to grow abalone from abalone hatching to spore production, Nutrition, and the growth of abalone more than natural feed, and enables stable supply.

Therefore, if the diets of the present invention prepared as described above are fed to abalone, realization of environmentally-friendly abalone breeding can strengthen immunity, prevent diseases and prevent diseases, grow healthy without infecting diseases and improve the growth rate, The feed efficiency can be increased.

The present invention can replace Spirulina fish meal and wheat flour as an environmental friendly abalone feed additive using horns as an economical additive as a seaweed which is discarded because of its low commercial value but in terms of nutrition, It can reduce the unit price for the circle and improve the price competitiveness of the abalone.

As a result, the use of the present invention ultimately provides a high-quality mixed feed for the whole body, thereby promoting the growth of abalone and reducing the loss of feed due to an increase in the feed intake rate, thereby improving productivity by reducing feed costs, It is possible to make the environmentally friendly style of healthy abalone possible.

While the present invention has been described in connection with certain exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. .

Claims (2)

An overturned feed additive comprising seaweeds,
An abalone feed additive containing fermented mash mushroom extract as an active ingredient,
After immersion and washing, the vacuum-dried moth is finely pulverized, and then the fermenting microorganism bacillus subtilis (Bacillus subtilis) is used to effect the fermentation microorganism activity for 20 to 30 hours at a temperature of 20 ° C to 40 ° C Fermented mungbean extract obtained by low temperature fermentation and fermented mashbang extract is mixed with a crude herbal medicine extract, bokbunja extract, white mulberry extract, early stage extract and fermented extract of Saururus chinensis extract as an active ingredient in one or more equivalent ratios at a total weight ratio of 10 : 1, wherein the composition comprises a fermented mash mash extract as an active ingredient, an abalone feed additive
The method according to claim 1,
Wherein the fermentation plant complex extract comprises at least one of 1-90% by weight of the same amount of the extract of the plant combination extract, the extract of Crassulaceae, the extract of Crassulaceae, An abalone feed additive containing an extract as an active ingredient
KR1020150190957A 2015-12-31 2015-12-31 Feed additives for abalone KR20170079910A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200059724A (en) * 2018-11-21 2020-05-29 한국프라임제약주식회사 Feed additive compositions for immuno-enhancement comprising sargassum horneri garbage and methods for their production
KR102385404B1 (en) * 2021-12-02 2022-04-12 대한민국 Method for culturing of abalone using Sargassum horneri
KR102502267B1 (en) * 2022-04-08 2023-02-23 대한민국 Method for manufacturing fermented feed using sargassum horneri

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200059724A (en) * 2018-11-21 2020-05-29 한국프라임제약주식회사 Feed additive compositions for immuno-enhancement comprising sargassum horneri garbage and methods for their production
KR102385404B1 (en) * 2021-12-02 2022-04-12 대한민국 Method for culturing of abalone using Sargassum horneri
KR102502267B1 (en) * 2022-04-08 2023-02-23 대한민국 Method for manufacturing fermented feed using sargassum horneri

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