KR20170083749A - The process of making healthcare compositions comprising fermented Cudrania tricuspidata extracts and seaweed extrcts - Google Patents

Abstract

The present invention relates to a functional combination extract for abalone feed comprising a mixture of a mixed fermented extract of cedarwood and algae. The extract is prepared by pulverizing the dried cucurbit tree leaf, kelp and natural herbal herb, followed by pulverization and air classification in the air classifier (Bacillus subtilis, Bacillus subtilis), which is a fermented microorganism, was used as a raw material of the micro powder mixture of the cucumber tree, kelp, kelp and natural herbal medicine, The present invention relates to a functional combination extract for a poultry fermentation abalone fermentation, which is obtained by mixing a mixed fermented extract of Cajunprimae and algae extracted by low temperature fermentation under the action of low temperature fermentation.

Description

Technical Field [0001] The present invention relates to a functional composite extract for abalone feed, which comprises a combination fermented extract of Cucurbitaceae and a seaweed,

The present invention relates to a functional complex extract for abalone feed in which a mixed fermented extract of a barnyardgrass and a seaweed is mixed,

More specifically, the washing vacuum dried cucurbitaceous tree leaves, kelp and natural herbal herb were first pulverized and then pulverized again. The air was classified in an air classifier to give an ultrafine powdery cucumber tree and sea tangle , A mixed fermented extract of coconut palm tree and seaweed, which is obtained by fermenting a mixture of natural powdered natural herb medicine and cocoa powder and algae using a fermenting microorganism, bacillus subtilis (Bacillus subtilis) The present invention also relates to a functional combination extract for poultry fermentation of an abalone feed which has enhanced the pharmacological activity of alfalfa and algae.

Abalone is a high-value food cultivated from ancient times as a high-priced preference food with industrial prospects. Abalone's body is traditionally high-quality fish food and inner shell surface with iridescent pearl luster is used as a craft for high-grade mother-of-pearl. And abalone farming is also used as a seed for pearl farming.

Recently, abalone farming industry in Korea has grown greatly, but the disease of abalone during farming has been a crucial factor for hindering development of abalone farming industry.

In recent years, abalone farming has mainly been carried out by artificial nursery production, in which the seedlings are discharged to the reef area and re-harvested in the size of the commodity. The abalone is cultivated in the aquaculture tank as a compound feed to improve the survival rate and growth rate of abalone. The most important consideration in the aquaculture is proper water temperature and sufficient food supply, and it is possible to maintain the proper water temperature of abalone at 18 ~ 22 ℃ in aquaculture tank style. Has become an important factor.

Stable supply of feed is becoming a big obstacle in abalone farming. In other words, abalone cultured fish are mainly used for nourishing food such as seaweed and raw seaweed, and they supply dried seaweed, kelp, and salted seaweed in the summer season.

In the abalone diet of natural feed, feed is the main factor that determines the success of the aquaculture. It not only grows abalone but also affects water pollution and economic efficiency. When nourishing food is used for natural food such as camphor and raw kelp It is reported that the growth effect is lower than that of the compound feed because the production supply is unstable due to the timing and local circumstances and the price fluctuation is severe and the main nutrient content is lower than the required amount for normal growth of the abalone.

Especially, fishermen who cultivate abalone are using natural food such as seaweed, parasites, and kelp as food for breeding, and they supply dried seaweeds and dried kelp in summer, which is a limiting factor for systematic aquaculture.

Currently, abalone is dependent on seaweed and kelp. It needs about 600,000 tons of seaweed for the production of 30,000 tons of abalone, but the production of algae is not much bigger than that for abalone production.

In other words, the supply of natural food is not only unstable but also has a disadvantage of fluctuating price and it is also reported that the growth rate is lower than that of mixed nutrients which have various nutrients required for aquaculture. Therefore, it is urgently required to supply alternative feeds for abalone cultivation which can produce more economically and at the same time, growth of abalone is advantageous

Seaweeds are classified into red algae, green algae and brown algae according to their color, photosynthetic pigment. Brown algae include seaweed, kelp, tortoise, mackerel, tortoiseshell, crab, and seal. Red algae refers to such things as Kim, mugwort, and squid. The green algae have something like flower buds, parasites, and hearing.

In addition, the excellent pharmacological effects of seaweeds have been clarified and the interest of modern people in seaweeds has been increasing. Seaweeds have 20 species in the Pacific coast, and carbohydrates such as mannit and raminanin, inorganic salts such as iodine, potassium and calcium, It is composed of substances beneficial to the human body and has been edible since ancient times

As a seaweed, kelp has been widely used as a food additive, which is widely used as a seasoning substitute, and is widely known as a long-lived food that prevents various adult diseases. Traditionally, kelp has been used for various purposes such as iodine, alginic acid, calcium, laminin, potassium, taurine, fucoidan, Minerals and other nutrients rich in nutrients as a report of eating a lot of it, constipation, diabetes, arteriosclerosis, osteoporosis, hypertension, and can prevent various adult diseases

In addition, kelp is one year old brown algae growing at 2 ~ 3m. It is famous as a natural food containing various nutrients. In particular, ingredients extracted from kelp are known to exert excellent effects such as red tide removal and fish parasitic elimination.

The ingredients extracted from kelp are not only low in manufacturing cost but also nontoxic natural substances, so they do not leave secondary pollution or toxicity even if they are administered for a long time in a large amount.

Cucumber tree is widely distributed in East Asia including China and Japan. It is known as a deciduous tree of mulberry trees and has various pharmacological actions. Recently, the fruit trees that have been spotlighted by their scientific discoveries, It is a kind of organic agricultural product known as fruit that does not suffer from pests because of the immunity of its own immunity. So it is said that there is a story that cucumber trees have eaten fresh from the past. And has an excellent effect of treating various diseases.

Also, it is a healthy functional food that is newly emerged so that the stem, leaf, skin,

      It is known that the fruit of the cucumber tree is ripened red lump in September and it is known to be excellent in its taste. As a result, Which has been used for the treatment of hypertension and gynecological diseases, has been known for its excellence in a number of ancient books such as Donguibogam, Phytoplankton,

In recent studies, it has been found that Cucumber leaf has higher nutrient and physiological activities such as protein, dietary fiber, vitamin B1, vitamin B2, calcium, iron, potassium, GABA and rutin than green leaf, common mulberry leaf, The material content is high,

Mulberry Fruit It is a fruit that is bigger than red and has a red color. It contains a large amount of asparaginic acid, glutamic acid, flavopiravin, vitamin C and minerals as well as antioxidant components such as flavonoids such as GABA, MAURIN, Therefore, according to the literature data, various functional materials such as antimicrobial and antioxidant activity, antidiabetic, antihyperlipidemic, antihypertensive, anti-cytotoxic, etc. are mainly found in fruit trees, And the extracts of leaves, fruits, and stems of Cucumber trees were found to be excellent for preventing adult diseases such as anticancer effects.

Therefore, the above-mentioned cypress tree extracts a variety of various effective components through the analysis of the composition components. Recently, various health promotion and therapeutic effects have been known, and the use thereof has been gradually expanded to use various new applications such as functional beverages and medicines It is pioneering.

Although various pharmacological activities have been actively carried out to maximize the efficacy of cucurbitan, cucurbitan has a function as a medicinal herb, but it is not easy to ingest, and it is difficult to process raw materials, and its pharmacological activity per unit weight is not strong The development of functional foods that are stronger than the pharmacological effects inherent in herbal medicine is required.

In addition, feed additives are supplementary feeds that are added to feeds to feed the essential nutrients or increase the productivity by achieving specific effects. Specifically, the feed additives are used as food for the purpose of supplementing deficient water, improving feed efficiency, It is an additive.

Therefore, the abalone feed industry has made a lot of efforts to maximize the efficiency of feed, and the development of various feed additive capable of performing additional functions such as improving digestion function and absorption rate of abalone are important in the feed industry .

However, since the extracts extracted by the conventional method are hydrothermally extracted at a high temperature, the useful components contained in the raw material are deformed and destroyed due to the heat at high temperature, and thus the effect of the useful ingredient is reduced

In the simple grinding extraction method, since the cell wall is strong, many cells are not destroyed, and they are left intact.

Accordingly, the present inventors have made a lot of efforts to maximize the efficiency of the whole feed, and as a result, the present inventors have developed a functional combination extract for abalone feed, which is a mixed composition of a mixed fermented extract of cucumber wood and algae.

The prior arts which have introduced the prior arts are as follows, and the prior art is also mixed with the extract of the cedar mulberry tree, the sea tangle and the medicinal herb which have been proved to have the functional effect so that they can complement each other without deteriorating the physical properties, , Nutritional functionality, sensory tastiness, and health promotion function effects such as resistance to various diseases of active ingredients of each function are simultaneously implemented to satisfy the needs of consumers at the same time. I have a common problem that I can not do

Document 1: Korean Patent Laid-Open Publication No. 10-2013-0016679

(Composition for preventing or treating neuronal damage comprising extracts of Cucurbitaceae leaves, date of publication February 18, 2013)

Document 2: Korean Patent Laid-Open Publication No. 10-2015-0144042

(Method for producing feed using seaweed, published on December 24, 2015)

As described above, the overturned feed additive of the prior art has various problems,

Compared to the conventional extraction method, it has an excellent anticancer activity to be easily absorbed in vivo according to the characteristics by the improved extraction method. Also, it has a high antioxidant activity, The effect is more enhanced than that of the pharmacological effect, and the composition is mixed and formulated at a proper concentration so as to complement each other without losing the physical properties or the active ingredients,

The present invention solves the technical problem of developing an economical high-quality abalone feed additive which not only promotes the growth of abalone, but also increases the resistance to changes in water quality and disease, thereby promoting the growth of abalone and improving the growth rate. It is.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems of the prior art,

Washing Vacuum Dried cacao tree, leaves, kelp and natural herbal medicine crude were crushed, then pulverized again and classified by air in an air classifier to give an ultrafine powdered cocoa tree, which was destroyed by cell walls, mixed with kelp and natural herbal medicine The fermented microorganism Bacillus subtilis (Bacillus subtilis), which is a fermented microorganism, is fermented by low temperature fermentation and extracted from a mixed fermented extract of Cajunprima and algae. And to provide a functional complex extract for overproduction of poultry fermentation which has enhanced pharmacological activity of algae.

As described above, according to the present invention, the combined functional extract for abalone feed,

Antioxidant, anti-cancer, anti-bacterial, anti-inflammatory and antidiabetic effects inherent in the extract of tree extracts, and the active ingredients of brown sea tangle, complex herbal medicine The disease control, the disease recovery, and the supplementary function are added, and it is possible to replace the chemical antibiotic, and besides the nutrition balance, the nutrient and the vitamin inorganic substance which are insufficient in the conventional abalone feed are supplemented As a composition for growth promotion having effects of improving feed efficiency, increasing body weight, activating digestive enzyme secretion, increasing immunity, improving breeding productivity, antimicrobial activity, and antioxidant, To promote digestion and improve survival rate and growth rate , The growth of abalone is promoted, so that healthy abalone can be cultured, and thus the efficiency of the feed can be maximized.

Especially, extraction of effective ingredients such as coconut shellfish and kelp is to break down strong cell walls such as trees and kelp, to extract as much effective ingredients as possible from the raw materials and to use them as effectively as possible. It is possible to maintain high quality by keeping the natural ingredient at a minimum, it is easy to store for a long period of time, and the pharmacological effect is enhanced by including the herbal medicine of the multi functional ingredient.

The present invention can substitute spirulina meal and wheat flour as an environmental friendly abalone feed additive using fermented tuna as an economical additive, thereby reducing the cost of protein source of abalone feed, thereby improving the price competitiveness of abalone.

In other words, the present invention can meet the nutritional requirement of abalone required for rapid growth by adding fermented sea tangle as an algae extract and replacing an animal protein with a plant protein, thereby enhancing growth and affecting body composition, To improve the adaptability to the environmental change of the abalone, or to increase the bioactivity of the active ingredient against the abalone, thereby improving the utilization efficiency of the feed as a whole.

In particular, the feed additive of the present invention includes a fermented tuna fermented by adding a strain of Bacillus genus having alginic acid decomposing ability and antibacterial ability to a feedstuff containing kelp, and when the feed is fed to abalone, the effective ingredients The bioavailability of the abalone is increased, the amount of the abalone is increased, and the antimicrobial substance is included, so that the immunity against the pathogenic bacteria of the fish is increased, thereby improving the abalone growth rate and survival rate.

In order to achieve the above object, the present invention will be described in detail.

The present invention relates to a functional complex extract for abalone feed in which a mixed fermented extract of Cucurbitaceae and seaweed is mixed,

Washing Vacuum Dried natural oriental herbal medicines consisting of coconut tree leaves, sea tangle extracts, extracts of bamboo shoots, extracts of Bacillus sieboldii, extracts of the early stages and extracts of Saururus chinensis were each pulverized with a pin mill and then pulverized again in a Turbo mill. A classification device was used to classify the micropowder mixed material of cucurbit and seaweed, which is a mixture of cucumber tree, kelp and natural herbal medicine in a weight ratio of 1: 1: 0.1, The mixed fermented extract of Cajunprima and Seaweed, which is fermented by low temperature fermentation under the action of fermenting microorganism for 20 to 30 hours at a temperature of 20 ° C to 40 ° C using bacillus subtilis (Bacillus subtilis) And functional complex extracts for the upturned feed of fermented fermented poultry with enhanced pharmacological activity of algae .

According to the present invention, the combined fermentation extract of Cucurbitaceae and seaweed is a low-temperature extraction method for optimizing an effective ingredient, and fermenting leaves and algae to enhance the pharmacological activity of the cucurbit tree. In the fermentation process, The cell membrane is destroyed and the microorganisms are finely crushed to contain the active ingredient dissolved in water and the dissolved fermentation product is formed so that it can be easily absorbed in the body. Since the temperature is lower than that of the heat extraction method, the oil component is destroyed or deteriorated by heat .

In the process of manufacturing the functional composite extract for abalone feed, the crushing of the cucurbitan leaf, kelp and herbal medicine is first pulverized to a diameter of about 1 mm by using a pin mill, and then a beater is prepared in a turbo mill. The pulverization was performed at a rotating speed of 6,750 rpm and air classification was performed at an air classifier at a class wheel speed of 5,000-7,500 rpm to extract the ultrafine powder as a cell wall.

In the present invention, the combined extracts of the above-mentioned Codonopsis lanceolata extract, Algae and Oriental herb medicine are mixed at a mixing ratio (weight ratio) of 1: 1: 0.1 to extract the complex extract, In order to induce a more effective state for this ingestion,

The microorganism may be at least one food fermentation selected from the group consisting of Aspergillus oryzae, Aspergillus kawauchi, Aspergillus niger, or hiochibacteria in addition to bacillus subtilis (Bacillus subtilis) Microorganisms can be selected, and the microorganisms can be obtained by using a microorganism existing in a natural state, a commercially available microorganism or a microorganism deposited in a microorganism depository.

In particular, the present invention eliminates the conventional heat extraction method and extracts the fermented extracts of cucumber tree, algae, and herbal medicines in which the pharmacological activity is enhanced by fermentation by fermentation microorganism or actinomycetase in the fermentation tank at low temperature, The beneficial physiologically active ingredients are increased, and various effects such as antioxidant effect, antioxidant, anticancer, antibacterial, anti-inflammatory, anti-diabetic and anti-atopic effect are promoted and the loss of components is minimized without heat treatment, Which is effective for improving the pharmacological effect of the active ingredient of the present invention.

The composition of the present invention, which has been mixed and compounded as described above, is preferably added as an essential ingredient to be normally added as an abalone feed additive considering the physiology of abalone, and it is preferable to add 5 to 25% by weight in the total weight ratio in the abalone- Functional food additive composition is added in an appropriate amount in accordance with the overturning growth step in a compounded diet comprising protein, carbohydrate, lipid and fatty acid, minerals as nutrients, and various vitamins, Increase digestive power and immunity, and form an environmentally friendly compound feed close to the most natural food with optimal taste and nutrients. Underwater corruption at high temperature is less, preventing feed loss and water pollution, enabling eco-friendly form of healthy abalone. do.

When the feed additive of the present invention is added to the overturned combination feed, if the content of the feed additive is too low, the effect of addition is small, and if the added amount is too large, it becomes uneconomical and not preferable. It is 15% by weight based on the total weight of the overturned chewing compound feed containing the functional feed additive.

In addition, the functional complex extract for abalone feed in which the complex fermented extract of Cucurbitaceae and Algae according to the present invention are mixed can be extracted with only the fermenting microorganism, but it is preferable to add the alanase enzyme. When the enzyme is fermented by adding the enzyme to the microorganism under the above-mentioned conditions, the enzyme can proceed the microbial fermentation more quickly. The main raw material of the present invention, cucumber tree, is a raw material containing a large amount of antibacterial component. The reaction may not be performed well. Therefore, it is preferable to add a SANYODACHI enzyme to the microorganism in order to solve this problem.

Recently, its efficacy has been scientifically revealed, and the cucumber tree is our unique plant resource. Due to the immunity of the plant itself, it is an organic agricultural product known as a fruit that does not suffer pests even without pesticides. And it is a health functional food which is newly emerged so that the stem, leaf, skin and fruit are all used as a medicine because there is no poison. It has a different taste and different pharmacological activity depending on the site of use,

In order to increase the antioxidant capacity of the cucurbitan tree, the cucumber tree has the highest antioxidant power and anticancer power among the cucurbit tree species, Leaves were used.

The cucumber tree is a plant herbal medicine which has been proven to be safe for a long period of time. Therefore, the fermented product according to the present invention, which is made from the plant herbal medicine, has a merit that a more effective pharmacological effect can be obtained without various side effects due to the use of the drug.

According to the present invention, the above-mentioned conditions for producing the fermented extract of Cucurbitaceae may be an optimization condition for fermentation. If these ratios are changed, the extraction efficiency of the active ingredients of Cucurbitaceae through fermentation extraction process may be lowered or the flavor may be significantly lowered.

It is widely used in oriental medicine and herbal medicines. Due to its diversity and functionality, attention has been focused on the development of preventive and therapeutic materials for dementia, and various studies have been conducted. The inhibition of brain accumulation of acetylcholine esterase inhibitor, Improvement of oxidative stress, effect of estrogenic effect and the like. In the present invention, a large amount of useful microorganisms are contained, and not only pharmacologically excellent effects are exhibited, but also preference is improved, so that anyone can easily ingest them .

The brown algae including seaweeds are rich in polysaccharides such as alginic acid, fucoidan, agar and porpharan to suppress the absorption of cholesterol, adsorb and discharge heavy metals, Fucoidan, an acidic water-soluble polysaccharide, has been proved to have anticoagulant activity, antitumor anticancer activity, and antioxidant effect, and iodine, calcium, And hematopoiesis.

Fucoidan, a physiologically active substance of brown algae, is a substance that induces apoptosis leading to death of cancer cells, inhibits angiogenesis that inhibits cancer growth, inhibits platelet adhesion of cancer cells to prevent cancer metastasis, And immunity enhancement function are excellent, and it is getting popular as a natural anti-cancer drug.

The content of the complex extract, which is mixed with the extracts of the present invention and the five complex natural oriental herb medicine extracts such as bokbunja extract, white mulberry extract, early-stage extract and saururus chinensis extract, is not limited. , And may contain 1-90% by weight of each of the extracts of Bombyx mori, Rubus corea extract, Bacillus subtilis, and Saururus chinensis extract.

In the present invention, the herbal medicine complex extract is a composition for accelerating abalone growth, which comprises at least one of natural extract as a natural herb medicine, bokbunja extract, white mint extract, early stage extract and Saururus chinensis extract as effective ingredients. The present invention provides a method for improving the survival rate and the growth rate of abalone to improve the ability to infect and improve healthy abalone. In particular, in the present invention, among a plurality of plant- ) It is composed of a herb medicine which increases the production amount and is formed at an optimum compounding ratio. It stimulates bioprotein synthesis and cell differentiation by IGF (growth factor) promoting effect and promotes the growth of abalone and promotes immune function

The herbal medicine used in the present invention is widely used as a medicament since the medicinal effect is described in detail in the medicinal herb medicine.

Glucoside and saponin are the main ingredients of astragalus, and they are used as expectorants for bronchitis, and are effective for anemia, insomnia,

Bokbunja is a medicinal herb of wild raspberries belonging to Rosaceae. It has antioxidant, anticholesterol, liver and gastrointestinal function improvement. Its efficacy is mentioned in various documents such as Dongbibogam, In pharmacological analysis of medicine, it has been found that polyphenol is contained in a large amount in the fruit, anti-cancer effect, anti-aging, prevention of arteriosclerosis, prevention of thrombosis,

Cynanchum wilfordii belongs to the family Polygonaceae

It is a perennial herb, with a height of 1-3 m and its roots are thick roots. The main ingredients of white seaweed are cinanotoxin, phytotoxotoxin, etc., and it is used as a tonic and anti-

Phlomis umbrosa is a 1 m high perennial herb belonging to the Lamiaceae family, with a height of 70-150 ㎝ and a height of about 5 root. Leaves are used for food, roots and rootstocks are used for medicinal purposes. It is known to have pharmacological effects to protect the liver and kidneys. It contains alkaloids, essential oils and vitamins.

The natural herbal medicine used in oriental medicine is low toxic or non toxic, does not remain in the body and does not cause drug tolerance. Herbal medicine herbal medicine has been used to treat various diseases. Especially when using herb medicine, Which has a strength in the prophylactic part.

As used herein, the term 'extract' has the meaning of being used as a crude extract in the art, and the composition ratio of each ingredient can be added or subtracted within a usual range when the feed additive of the present invention is manufactured.

In the present invention, the washing and drying of the cedarwood leaves, kelp and natural herbal medicine herbal medicine are purified water. In the present invention, the impurities are removed by washing with functional water containing a nano bubble having sterilization, antibacterial effect, antiparasitic effect, In the far-infrared sterilizing drying apparatus of the drying function, the safety of the composite raw material according to the present invention is secured by the method of drying at a moisture content of 13-14% for 2 hours by using a far-infrared ray drying sterilizer at a temperature of 40-50 ° C, It is desirable to meet the acceptance criteria for harmful substances such as toxic substances.

That is, the above-mentioned purified water of the present invention is a bubble of nano-pore size, which is very fine bubbles having a diameter of 1 μm (1 micrometer: one millionth of a meter) or less containing water containing minute oxygen bubbles which are not visible to the naked eye, bubbles can be present in the liquid over a long period of time, thus performing a complex action such as sterilization and deodorization as well as purification of water. In addition, the disinfecting action of microbes with high purity and the disinfection action of disruption- .

It is desirable to dry far-infrared rays from the leaves of Cucumis japonica. It is desirable to dry far-infrared rays. Cucurbitaceae leaves are dried by far-infrared rays, so that low-molecular antioxidants present in cucurbitaceae leaves are liberated, thereby maximizing antioxidant power and sterilizing, sterilizing, So that the color and flavor are alive, and the shelf life can be improved by not rusting.

Therefore, when the compound feed additive of the present invention manufactured as described above is mixed with the general compound feed at a proper ratio and fed to the abalone, realization of environmentally friendly abalone breeding will be performed to strengthen the immunity and to prevent and treat various diseases, And the growth rate can be improved, so that the mortality rate can be reduced and the feed efficiency can be increased.

Example 1. Production of functional feed additives

1-1. Manufacture of cedarwood tree extract

The collected nursery trees were screened, washed, dried and finely pulverized. Nursery tree leaves were finely pulverized with a fin mill, then finely pulverized again in a Turbo mill, air classification was performed in an air classifier, and ultrafine powder I got a cranberry tree extract.

1-2. Preparation of kelp extract

Selective Washing The dried raw materials of the dried and finely divided kelp are added to the above 1-1. , Followed by pulverization again in a turbo mill and air classification in an air classifier to obtain an ultrafine powdery kelp extract having cell walls destroyed.

1-3. Manufacture of herbal medicine herbal medicine complex extract

(1: 1: 1: 1) composition ratio (weight ratio, equivalent ratio) of raw extract, bamboo extract, white sachet extract, early stage extract and Saururus chinensis extract were mixed and fermented. , Followed by pulverization again in a Turbo mill and air classification in an air classifier to obtain an ultrafine powder type herbal medicine complex extract having cell walls destroyed.

1-4. Preparation of Functional Feed Additives including Extract of Curcuma longa and Seaweed Extract and Oriental Herb Medicine Extract

1-1. Cranberry extract of the process, 1-2. (1: 1: 0.1 ratio) of raw seaweed extracts and herbal medicine extracts obtained in 1-2. Process were mixed with the microfine powder mixture of Bacillus subtilis (Bacillus subtilis, ) Was used for fermentation for 20 to 30 hours at a temperature of 20 ° C to 40 ° C to ferment and fermented at a low temperature to produce a mixed fermented extract of cucurbitu and seaweed to enhance the pharmacological activity of the cucurbits and algae A functional complex extract for overturning feed of fermentation broth was prepared

Example 2. Manufacture of abalone formula containing functional food additive

The abalone feed additive obtained in the above step 1 was added to the prepared feed mixture in an amount of 15 wt%: 85 wt% based on the total weight ratio, and then mixed with stirring to prepare an abalone mixed feed supplemented with the functional feed additive of the present invention .

Test example:

The commercial off-the-blend diets supplemented with the functional feed additive of the present invention produced in Example 2 were prepared as feedstuffs for four weeks without any addition of the functional additive of the present invention, and the feed intake rate, growth rate, The feed efficiency of the present invention was investigated. The compound feed added with the present invention showed a higher survival rate and a higher growth rate than the feed without the functional additive of the present invention,

The feed efficiency was significantly higher than that of the control diet. Therefore, it was found that the abalone feed supplemented with the present invention realized remarkable intake and digestibility and improved survival rate.

As a result, the use of the present invention ultimately provides a high-quality feed additive for the whole body, thereby promoting the growth of abalone and reducing the loss of feed due to an increase in the feed intake rate, thereby improving the productivity by reducing the feed cost, By solving the marine pollution problem, it was found that the economical and environmental friendly form of healthy abalone could be made possible.

Therefore, the overturned feed additive of the present invention significantly reduces the stress of the abalone, and the appetite becomes strong, and the growth rate and the disease prevention rate are remarkably improved, so that it is necessary to grow abalone from abalone hatching to spore production, Nutrition, and the growth of abalone more than natural feed, and enables stable supply.

Therefore, if the diets of the present invention prepared as described above are fed to abalone, realization of environmentally-friendly abalone breeding can strengthen immunity, prevent diseases and prevent diseases, grow healthy without infecting diseases and improve the growth rate, The feed efficiency can be increased.

While the present invention has been described in connection with certain exemplary embodiments, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. .

Claims (2)

An overturned feed additive comprising coconut trees and algae,
Washing Vacuum Dried natural oriental herbal medicines consisting of coconut tree leaves, sea tangle extracts, extracts of bamboo shoots, extracts of Bacillus sieboldii, extracts of the early stages and extracts of Saururus chinensis were each pulverized with a pin mill and then pulverized again in a Turbo mill. A classification device was used to classify the micropowder mixed material of cucurbit and seaweed, which is a mixture of cucumber tree, kelp and natural herbal medicine in a weight ratio of 1: 1: 0.1, Characterized in that the fermented extract is extracted by low temperature fermentation using bacillus subtilis (Bacillus subtilis) at 20 ° C to 40 ° C for 20 to 30 hours under the action of a fermenting microorganism. Functional complex extract for abalone feed The method according to claim 1,
In the process of manufacturing the functional composite extract for abalone feed, the crushing of the cucurbitan leaf, kelp and herbal medicine is first pulverized to a diameter of about 1 mm by using a pin mill, and then a beater is prepared in a turbo mill. The mixture was finely pulverized at a rotation speed of 6,750 rpm and air classification was performed at an air classifier at a class wheel speed of 5,000-7,500 rpm to extract the ultrafine powder in which the cell wall was broken. The combined fermented extract of Cedarwood and Seaweed Functional complex extract for abalone feed