KR102235334B1 - Method for manufacturing calcium powder using abalone, oyster shell, calcium powder prepared thereby, and food, cosmetic, pharmaceutical products containing the same - Google Patents

Abstract

The present invention relates to a method for producing calcium powder using abalones and oyster shells, calcium powder produced thereby, and a food, cosmetic material, or pharmaceutical product including the same. The method for producing calcium powder using abalones and oyster shells comprises: producing abalone shells and oyster shells, and washing the same to remove foreign substances adhering to the abalone shells and oyster shells; firstly crushing the washed abalone shells and oyster shells to produce a granulated product of abalone shells and oyster shells; immersing the granulated product of abalone shells and oyster shells in a mixed solution; separating and taking out the granulated product of abalone shells and oyster shells from the mixture, and then heating and firing the granulated product of abalone shells and oyster shells; secondly pulverizing the fired abalone shells and granulated oyster shells, and removing impurities to finely produce a ground product of abalone shells and oyster shells; spraying and inoculating minerals and a lactic acid bacteria culture solution onto the ground product of abalone shells and oyster shells; fermenting the ground product of abalone shells and oyster shells inoculated with the mineral and the lactic acid bacteria culture medium; and washing, drying, and sterilizing the fermented ground product of abalone shells and oyster shells to produce calcium powder. According to the present invention, abalone and oyster shells are used to produce high-purity calcium powder, and the calcium powder is used for food, cosmetics, pharmaceuticals. Thus, it is possible to easily use high-purity calcium and greatly improve the absorption rate of calcium in a human body.

Description

Manufacturing method of calcium powder using abalone and oyster shell, calcium powder prepared thereby, and food, cosmetics, and pharmaceuticals containing the same (METHOD FOR MANUFACTURING CALCIUM POWDER USING ABALONE, OYSTER SHELL, CALCIUM POWDER PREPARED THEREBY, AND FOOD, COSMETIC, PHARMACEUTICAL PRODUCTS CONTAINING THE SAME}

The present invention relates to a method for producing calcium powder using abalone and oyster shells, and to foods, cosmetics, and pharmaceuticals including the calcium powder and the resulting calcium powder, and more particularly, high-purity calcium using abalone shells and oyster shells. A method for producing calcium powder using abalone and oyster shells that can easily use high-purity calcium and greatly improve the absorption rate of calcium in the body by manufacturing powder and using it in foods, cosmetics, and pharmaceuticals, and calcium produced thereby It relates to powders and foods, cosmetics, and pharmaceuticals containing the same.

In general, calcium is the most ingested mineral among the minerals that make up the human body, and although it is abundantly contained in food materials, it is difficult to absorb and is always susceptible to lack of calcium.

The reason for the lack of calcium in modern people is that the acidification of the soil, which is the base of food production, excessive intake of fat, preference for instant food, and excessive intake of phosphate compounds due to excessive drinking of soft drinks causes the absorption of calcium. Silver is supplemented with calcium compounds that cannot be absorbed, namely calcium phosphate, calcium carbonate, calcium lactate, and powders such as shell powder and shellfish.

Calcium that exists in natural products exists in the form of compounds, and these are absorbed and metabolized by atomic particles rather than molecular, so the intake of calcium as a natural product has a fundamental problem.

Calcium in the body is not only for bone diseases such as skeletal and tooth formation, blood coagulation, muscle contraction and relaxation, nerve transmission, nerve excitation, cell membrane permeability control, vitamin B12 absorption, cell membrane fusion and division, and osteoporosis. It is also an important food and medicine material in reducing the occurrence of hypercholesterolosis, arteriosclerosis, hyperlipidemia, and high blood pressure. Recently, the demand for calcium-fortified foods around the world is increasing rapidly, and the number of calcium-fortified foods is steadily increasing year by year.

Currently, chemical synthetic products (calcium carbonate, calcium lactate, calcium citrate, etc.) and imported natural calcium agents (seaweed calcium, whey calcium) are representative of the currently used calcium agents in Korea, and natural calcium agents depend on imports for more than 80%. . Shellfish, oyster shells, starfish and egg shells that can be used as domestic natural calcium materials are collected every year, but their utilization is very low, and due to the occurrence of treatment costs and environmental pollution, there is a desperate need for a method of utilization.

Abalone shells and oyster shells are made of natural calcium, and the main component is calcium carbonate, which is a material that can be used in various ways, but the production of products is insufficient.Therefore, industrialization of food and industrial calcium using abalone shells and oyster shells is an eco-friendly, high value-added industry. In Japan, it is used as a calcium raw material, chicken feed additive, water purifier, and chalk manufacturing, but domestic product development is partially used in chicken feed.

Korean Patent Publication No. 10-2016-0080373 (published on July 08, 2016) Domestic registered patent No. 10-1356745 (registered on January 22, 2014) Domestic registered patent No. 10-1827456 (registered on February 02, 2018)

The present invention manufactures high-purity calcium powder using abalone shells, oyster shells, etc., and by using it in foods, cosmetics, medicines, etc., abalone, which can easily use high-purity calcium and greatly improve the absorption rate of calcium in the body, It is to provide a method of manufacturing calcium powder using oyster shells, calcium powder prepared thereby, and foods, cosmetics, and pharmaceuticals including the same.

Various problems to be solved by the present invention are not limited to the problems mentioned above, and other problems that are not mentioned will be clearly understood by those skilled in the art from the following description.

In the method for producing calcium powder using abalone and oyster shells according to the present invention, the abalone shell and oyster shell are prepared and washed to remove foreign substances adhering to the abalone shell and oyster shell, and the washed abalone shell and oyster shell are removed. The abalone shell and oyster shell granulated pulverized product is first pulverized, and the abalone shell and oyster shell granulated pulverized product are immersed in a mixed solution, and the abalone shell and oyster shell granulated pulverized product are separated from the mixed solution and taken out, and the abalone shell , The oyster shell granulated pulverized product is heated to be fired, and the calcined abalone shell and oyster shell granulated pulverized product are second pulverized, and then impurities are removed to prepare an abalone shell, oyster shell fine pulverized product, and the abalone shell, oyster After inoculation by spraying mineral and lactic acid bacteria culture medium to the fine-grained skin, fermenting the abalone shell and oyster shell fine-grained product inoculated with the mineral and lactic acid bacteria culture medium, and washing the fermented abalone shell and oyster shell fine-grained product. Calcium powder is prepared by drying and sterilizing.

In the step of immersing the abalone shell and oyster shell granulated pulverized product in the mixed solution, the mixed solution is 0.1 to 0.5% by weight of nitric acid, 0.5 to 1.5% by weight of phosphate compound, 0.01 to 0.1% by weight of persulfate, 1 to 3% by weight of acetic acid and the balance It is made of purified water, and the abalone shell and oyster shell granulated pulverized product may be immersed in the mixed solution for 10 to 30 minutes.

The phosphate compounds include first sodium phosphate (NaH ₂ PO ₄ ), second sodium phosphate (Na ₂ HPO ₄ ), third sodium phosphate (Na ₃ PO ₄ ), first potassium phosphate (KH ₂ PO ₄ ), and 2 potassium phosphate (K ₂ HPO ₄ ), first ammonium phosphate ((NH ₄ ) H ₂ PO ₄ ), second ammonium phosphate ((NH ₄ ) ₂ HPO ₄ ) and tertiary ammonium phosphate ((NH ₄ ) ₃ PO ₄ ) Any one or more selected from the group consisting of is used, and the persulfate is ammonium persulfate ((NH ₄ ) ₂ S ₂ O ₈ ), sodium persulfate (Na ₂ S ₂ O ₈ ) or potassium persulfate (K ₂ O ₈ S ₂ ) Any one or more selected from may be used.

After separating the abalone shell and oyster shell granulated pulverized product from the mixed solution, heating and sintering the abalone shell and oyster shell granulated pulverized product is 1,300 after putting the separated abalone shell and oyster shell granulated pulverized product into a sintering furnace. It can be carried out by heating for 30 to 200 minutes at a temperature of to 1,600 ℃.

In addition, the present invention includes a calcium powder using abalone and oyster shells prepared by the above-described method.

In addition, the present invention includes foods, cosmetics, and pharmaceuticals containing calcium powder using the abalone and oyster shells.

Details of other embodiments are included in the detailed description.

Calcium powder using abalone and oyster shell according to the present invention prepares high-purity calcium powder using abalone shell, oyster shell, etc., and uses it for food, cosmetics, medicine, etc., so that high-purity calcium is easily used and It can greatly improve the absorption rate in the body.

It will be fully understood that the embodiments of the technical idea of the present invention can provide various effects not specifically mentioned.

1A and 1B are photographs showing changes in state before applying the cosmetic prepared according to Example 1 to a 29-year-old woman (FIG. 1A) and after coating for 2 months (FIG. 1B).
2A and 2B are photographs showing changes in state before applying the cosmetic prepared according to Example 1 to a 33-year-old woman (FIG. 2A) and after coating for 2 months (FIG. 2B).
3A and 3B are photographs showing changes in state before applying the cosmetic prepared according to Example 1 to a 38-year-old woman (FIG. 3A) and after coating for 2 months (FIG. 3B).

Advantages and features of the present invention, and a method of achieving them will become apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided so that the disclosed content may be thorough and complete, and the spirit of the present invention may be sufficiently conveyed to those skilled in the art.

The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise.

Unless otherwise defined, all terms used herein including technical or scientific terms have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning of the related technology, and should not be interpreted as an ideal or excessively formal meaning unless explicitly defined in the present application. Does not.

Hereinafter, a method for producing calcium powder using abalone and oyster shells according to the present invention will be described in detail with reference to a preferred embodiment.

In order to prepare the calcium powder using the abalone and oyster shells according to the present invention, first, the abalone shell and the oyster shell are prepared and then washed to remove foreign substances adhering to the abalone shell and the oyster shell.

In the present invention, an abalone shell and an oyster shell are used as materials to produce calcium powder, but the technical idea of the present invention is not limited to the above-described abalone shell and oyster shell, but the abalone shell and oyster shell In addition, various materials containing calcium, such as conch, cockle, clam, petrification, and shellfish may be used.

In addition, in the step, the abalone shell and oyster shell are washed by washing the surface of the abalone shell and oyster shell using purified water in _{which sodium hydrogen carbonate (NaHCO 3) is dissolved,} , It is possible to remove foreign substances remaining on the surface of the oyster shell.

The sodium hydrogen carbonate (NaHCO ₃ ) is also used as a food additive, and is non-toxic and has functions such as penetration, diffusion, and expansion, and can cleanly clean the surface of abalone shells and oyster shells. The sodium hydrogen carbonate is 3 It has a concentration range of to 5 (w/w)%, and is preferably washed for 5 to 15 minutes at a temperature of 20 to 30°C.

Next, the washed abalone shell and oyster shell are first pulverized to prepare an abalone shell and oyster shell granulated pulverized product.

In the above step, the washed abalone shell and oyster shell are first pulverized using a granulator, so that impurities or organic residues inside the abalone shell and the oyster shell can be removed in a later process. It may be performed using a known granulator such as a pan granulator, a drum granulator, or a compression pelletizer, but is not necessarily limited to the granulator described above.

Next, the abalone shell and the granulated pulverized oyster shell may be immersed in the mixed solution.

In the above step, impurities or organic residues contained in the abalone shell and oyster shell granulated pulverized product may be removed by immersing the abalone shell and oyster shell granulated pulverized product in a mixed solution. Consisting of weight%, phosphate compound 0.5 to 1.5% by weight, persulfate 0.01 to 0.1% by weight, acetic acid 1 to 3% by weight, and the remaining amount of purified water, the abalone shell and oyster shell granulated pulverized product are mixed with the mixture for 10 to 30 minutes It can be carried out by immersion in.

In addition, as the phosphate compound in the above step, the first sodium phosphate (NaH ₂ PO ₄ ), the second sodium phosphate (Na ₂ HPO ₄ ), the third sodium phosphate (Na ₃ PO ₄ ), the first potassium phosphate (KH ₂ PO ₄ ), second potassium phosphate (K ₂ HPO ₄ ), first ammonium phosphate ((NH ₄ ) H ₂ PO ₄ ), second ammonium phosphate ((NH ₄ ) ₂ HPO ₄ ) and tertiary ammonium phosphate (( NH ₄ ) ₃ PO ₄ ) Any one or more selected from the group consisting of may be used, and the persulfate is ammonium persulfate ((NH ₄ ) ₂ S ₂ O ₈ ), sodium persulfate (Na ₂ S ₂ O ₈ ) Or potassium persulfate (K ₂ O ₈ S ₂ ) Any one or more selected from may be used.

Subsequently, the abalone shell and the oyster shell granulated pulverized product may be separated from the mixed solution and removed, and then the abalone shell and oyster shell granulated pulverized product may be heated and fired using a sintering furnace.

In the above step, by heating the separated abalone shell and oyster shell granulated pulverized product using a sintering furnace, contaminants adsorbed in the fine pores of the abalone shell and oyster shell granulated pulverized material can be removed and activated. The abalone shell and the oyster shell granulated pulverized product may be added to the sintering furnace and then heated at a temperature of 1,300 to 1,600°C for 30 to 200 minutes.

In addition, in the above step, calcium carbonate constituting the abalone shell and oyster shell may be dissolved into calcium oxide and carbon dioxide as follows by going through the firing process as described above.

Calcium carbonate (CaCO ₃ ) → Calcium oxide (CaO) + carbon dioxide (CO ₂ )

Next, the calcined abalone shell and oyster shell granulated pulverized product may be second pulverized and then impurities are removed to prepare the abalone shell and oyster shell fine pulverized product.

In the above step, the calcined abalone shell and oyster shell granulated pulverized product may be pulverized using a ball mill or various known pulverizers. It can be pulverized to be 1 to 10 μm.

In addition, in the above step, impurities such as the second pulverized abalone shell and heavy metals that may remain in the oyster shell are removed by second pulverizing the calcined abalone shell and oyster shell assembly pulverized product and then rotating it in a rotating cylinder equipped with a magnet. Specifically, the second pulverized abalone shell and oyster shell are inserted into a rotating container equipped with a magnet and then rotated at a rotation speed of 500 to 1000 RPM for 10 to 20 minutes, and the second pulverized abalone shell, Impurities such as heavy metals that may be included in the oyster shell can be removed.

Next, the abalone shell and oyster shell fine-grained pulverized product may be inoculated by spraying mineral and lactic acid bacteria culture solution.

In the above step, the abalone shell and the oyster shell may be sprayed at a weight ratio of 1 to 5 parts by weight of minerals and 5 to 10 parts by weight of the lactic acid bacteria culture solution based on 100 parts by weight of the fine pulverized material of the abalone shell and the oyster shell. It is a mineral that is absorbed by the pulverized skin of the skin and is a nutrient that is required in a relatively small amount for growth, maintenance and reproduction of the human body, and any one selected from the group consisting of calcium, phosphorus, potassium, magnesium, manganese, iodine, selenium, molybdenum and cobalt The above can be used.

In addition, in the step, the lactic acid bacteria may use a lactic acid strain isolated from kimchi, which is a fermented food, specifically, as the lactic acid strain, Lactobacillus curvatus , Weissella viridescens , Lactobacillus plantarum and Any one or more known lactic acid strains selected from the group consisting of Leuconostoc lactis may be used.

For example, in the above step, in order to prepare a lactic acid bacteria culture solution from kimchi, the pH of the medium is adjusted to 2.8-3.2 using 1.2N HCl, and then the lactic acid strain is separated from the kimchi, and the separated lactic acid strain is MRS broth (Oxoid , England) at 37~39℃ for 20 to 25 hours ^{, then diluted to 1×1.0 8} to 5×1.0 ⁸ CFU/mL, and then centrifuged at 10,000 to 15,000 rpm for 10 to 15 minutes, and the supernatant After separating only (supernatant), and filtering the supernatant with a 0.45 μm syringe filter, lactic acid bacteria are diluted by mixing and diluting at a weight ratio of 1,000 to 2,000 parts by weight of sterilized distilled water with respect to 100 parts by weight of the filtered supernatant. A culture solution can be prepared.

Subsequently, the abalone shell and the fine pulverized oyster shell inoculated with the mineral and lactic acid bacteria culture solution may be fermented.

In the above step, the abalone shell and oyster shell fine-grained pulverized product inoculated with the mineral and lactic acid bacteria culture solution may be fermented for 3 to 7 days after maintaining a temperature of 40 to 45°C and a humidity of 60 to 65%.

Next, the fermented abalone shell and oyster shell fine pulverized product may be washed, dried, and sterilized to prepare calcium powder.

In the above step, the fermented abalone shell and oyster shell fine pulverized product may be washed with purified water and then dried and sterilized. The configuration of the drying and sterilization process is a known technique, so for convenience of explanation and the present invention. For the sake of clarity of the technical idea, a detailed description thereof will be omitted.

The present invention can be used in foods, cosmetics, pharmaceuticals, etc. by using the calcium powder prepared as described above. For example, the calcium powder can be consumed by calcium nutrients or humans in livestock feed, and the following cosmetic composition is used. Can also be used.

Hereinafter, an example used as a skin whitening and moisturizing cosmetic composition using calcium powder prepared according to the present invention will be described in more detail.

The term "skin whitening" used in the present invention is a method of increasing the brightness of the skin whose brightness has decreased due to an excess of pigments such as melanin, or maintaining the brightness of the skin at a certain level, and the brightness formed by the above method It is meant to encompass increased skin and the like.

In addition, the term "skin moisturization" as used in the present invention suppresses the evaporation of skin moisture out of the body and blocks excessive penetration of foreign substances, thereby maintaining moisture in the stratum corneum and making the skin dry or rough. This means to prevent the skin from being soft and moist.

The cosmetic composition for skin whitening and moisturizing using the calcium powder prepared according to the present invention includes abalone, calcium powder using oyster shells, mascarpone extract, bringgraz oil, amra oil, jojoba seed oil, silkworm mature powder processing oil and jade powder. Includes.

In addition, the cosmetic composition for skin whitening and moisturizing using the calcium powder prepared according to the present invention includes 1 to 2 parts by weight of calcium powder using abalone, oyster shell, 0.1 to 1.5 parts by weight of Maesengyi extract, 0.05 to 0.15 parts by weight of Bringraz oil It may be included in a weight ratio of 0.05 to 0.15 parts by weight of amra oil, 0.01 to 0.05 parts by weight of jojoba seed oil, 0.1 to 0.2 parts by weight of silkworm aging powder processing oil, and 0.001 to 0.01 parts by weight of jade powder.

The calcium powder using the abalone and oyster shell may be used as the calcium powder using the abalone and oyster shell prepared as described above.

The Maesengi extract may be prepared using Maesaengi, and the Maesengi extract prepared by the following method may be used.

First, after preparing Maesaeungi, it can be first washed with purified water.

The Maesengi is a plant-based high protein food that evenly contains five major nutrients, and is a seaweed that contains a lot of nutrients except for lipids because it contains a lot of moisture, protein, carbohydrates, and ash.

In addition, the meesadian ear contains vitamins A and C, and contains various inorganic salts such as iron, calcium, iodine, etc., so it is effective in skeletal formation and prevention of osteoporosis for children's development, as well as hematopoietic function to prevent anemia. It is a nutritious food that can be used.

For example, in the above step, after preparing the Maesamyeongi, the Maesaengi may be washed with purified water at a temperature of 15 to 25°C, and foreign substances adhering to the Maesaengi may be removed by washing the Maesaengi using the purified water.

Next, the first washed Maesangyi may be immersed in an aging solution for a second washing.

In the above step, by immersing the Maesengi washed with the purified water in the aging solution to remove general bacteria present in the Maesaengi, it is possible to improve the storage stability of Maesaengi and remove the fishy smell that may occur from the Maesaengi.

For example, in the above step, first, green tea leaves, laurel leaves and purified water are mixed in a weight ratio of 0.5:1.5:8, and purified water in which the green tea leaves and laurel leaves are mixed is 20 to 75°C. After heating for 60 minutes, the green tea leaves and bay leaves are removed and filtered to prepare a filtrate. Next, the filtrate may be mixed with squid ink at a weight ratio of 9.5:0.5 and aged for 3 to 6 hours at a temperature of 10 to 15°C to prepare an aged liquid. Next, by immersing the Maesengi washed with the purified water in the aging solution and then storing them at a temperature of 5 to 7°C for 10 to 20 minutes, the storage stability of the Maesaengi can be improved and the fishy smell that may occur from the Maesaengi can be removed. I can. Subsequently, after separating the Maesaeungi immersed in the aging liquid, a second washing may be performed with purified water at a temperature of 15 to 20°C.

The squid ink refers to the contents of the squid musk sac, and is mainly black or dark brown.The squid ink contains mucopolysaccharides such as elexin, which have strong anti-tumor activity, in addition to anticancer effect, and promotes preservative action and gastric juice secretion. It can play a role in helping to work.

Thereafter, the second washed Maesamyeongi may be dried to remove moisture contained in the Maesaengi.

In the above step, the second washed maesaengyi can be stored and dried for 20 to 40 hours at a temperature of 45 to 50°C. If the step is performed below the above lower limit, the moisture contained in the mesaengyi There may be a problem in that it is not sufficiently removed, and if it is performed beyond the above upper limit, the content of water contained in the maeisaengi is too small, so the physical properties are deteriorated, and the aging fermentation of the maeisaengi does not proceed sufficiently in a later process. Can cause problems.

Subsequently, the dried Maesengi may be aged and fermented.

In the above step, it may be carried out by mixing the dermal fermentation broth with the dried meesengi and then aging fermentation for a certain period of time.In the above step, specifically, in the step, the dried mesentery is in a weight ratio of 1 to 5 parts by weight of the dermal fermentation broth with respect to 100 parts by weight. After mixing, by storing for 20 to 40 hours at a temperature of 8 to 12 ℃ can be fermented by aging the Maesaeungi.

The dermis fermentation broth may be prepared by fermenting the dermis (the peel of citrus), and the dermis is a variety of compounds such as polyphenols, vitamins, and limonoids, especially carotenoids, bio-flavonoids, pectins and terpenes. It is rich in useful ingredients such as phenolic compounds such as Ryu, and recently, it has been reported that hesperidin, an antioxidant, is separated from dermal by-products and fed to pig feed, resulting in improved disease resistance.

In the above step, the dermal fermentation broth may be used as the dermal fermentation broth prepared as follows.

First, in order to prepare the dermal fermentation broth, the dermis may be prepared and washed.

Washing in the above step may be performed using purified water in which sodium hydrogen carbonate is dissolved. That is, the sodium hydrogen carbonate (NaHCO ₃ ) is also used as a food additive, and is not toxic and may have functions such as penetration, diffusion, and expansion. In the above step, the concentration range of 1 to 3 (w/w)% Eggplant can sterilize and clean the surface of the dermis by using a sodium hydrogen carbonate solution. In this case, the dermis may be a portion of a citrus peel from which a top portion of the citrus mandarin orange has been removed.

Next, the dermis can be enzymatically degraded.

In the above step, the enzymatic decomposition can be decomposed using an enzyme capable of separating an immunoactive polysaccharide present in the dermis, preferably alpha-amylase, pectinase, xyl as the enzyme. Ranase (Xylanase) and cellulase (Cellulase) are used, the alpha amylase (α-Amylase) 0.01 to 0.03 parts by weight, pectinase (Pectinase) 0.02 to 0.04 parts by weight, xyl with respect to the total 100 parts by weight of the dermis Lanase (Xylanase) 0.03 to 0.05 parts by weight and cellulase (Cellulase) is mixed in a weight ratio of 0.02 to 0.04 parts by weight, and the enzyme is mixed at a temperature of 38 to 42 ℃ to be carried out by storing the dermis for 10 to 50 hours. I can.

Then, the enzymatically decomposed dermis can be stored and aged.

In the above step, the enzymatically decomposed dermis may be stored for 20 to 40 days at a temperature of 8 to 12°C. If the step is performed below the above lower limit, there is a problem that the dermis is not sufficiently aged. It may occur, and if it is performed in excess of the upper limit range, a problem of deteriorating physical properties of the dermis may occur.

Subsequently, by filtering the aged dermis using a filter, a filtered dermal liquid can be prepared.

In the above step, fine foreign substances contained in the dermis can be removed by filtering the aged dermis to prepare a filtered dermal liquid.For example, the filtration is performed by various known types such as a plate-type pressure filter or a cartridge filter. Filters can be used.

Next, the filtered dermal liquid is concentrated and sterilized to prepare a dermal concentrate, and the dermal concentrate is fermented to prepare a dermal fermentation liquid.

In the above step, a dermal concentrate is prepared using a vacuum concentrator known in the art, and vegetable lactic acid bacteria are mixed in a weight ratio of 0.1 to 0.2 parts by weight with respect to 100 parts by weight of the dermal concentrate, and then at a temperature of 10 to 15°C. By fermenting for 3 to 6 days, a dermal fermentation broth can be prepared.

In the above step, vegetable lactic acid bacteria are used to ferment the dermal concentrate, and known lactic acid bacteria capable of fermenting the dermal concentrate may be used as the vegetable lactic acid bacteria, for example, Lactobacillus plantarum as the vegetable lactic acid bacteria ( Lactobacillus Plantarum , 400 × 10 ⁹ CFU/g) can be used.

Next, the aging fermented meesaengi may be freeze-dried.

In the above step, the freeze-drying of the fermented meesengi may be performed at a temperature of -20 to -30°C.

Thereafter, the freeze-dried Maesengi may be pulverized to prepare Maesengi powder, and the Maesengi powder may be extracted to prepare a Maesengi hot water extract.

In the above step, after crushing the freeze-dried Maesengi to prepare Maesamyeongi powder, mixing in a weight ratio of 2,000 to 2,500 parts by weight of purified water to 100 parts by weight of Maesengi powder, and for 5 to 10 hours at a temperature of 90 to 95°C. By hot water extraction, it is possible to prepare a hot water extract of maesaengyi.

Subsequently, the mesaengi hot water extract may be freeze-dried.

In the above step, a freeze-dried meesengi hot-water extract may be prepared by freeze-drying the mesaengi hot water extract at a temperature of -40 to -50°C.

Next, the freeze-dried meesengi hot water extract may be extracted under reflux to prepare a meesengi extract containing useful ingredients of meesengi.

For example, the step may be performed using a known reflux extractor, and the extraction method using the reflux extractor is a known technique. For convenience of explanation and clarity of the technical idea of the present invention, a detailed description thereof will be provided. I will omit it.

The bringgraz oil has been used as a medicinal for enriching hair for centuries in India, and the bringgraz is also called nasturtium, and the eclipta prostrate is an annual herb of the chrysanthemum family with a height of 10 to 70 cm. It is a plant that occurs a lot in soybean fields in Jeju Island, southern regions, and central regions of Korea. From ancient times, it has been known to be effective in promoting blood flow, pain relief, immunity, dermatitis, promoting hair growth, and dermatitis where the skin is cracked. In particular, it is known to be effective in relieving skin irritation.

As the amra oil, a known oil extracted from amra may be used, and the amra is generally known as Indian gooseberry and deciduous arboretum. It is widely distributed in tropical and subtropical countries such as China, India, Indonesia, and Taiwan, and is known to contain vitamin C, minerals, amino acids, tannins, filamblic acid, rutin, curcuminoids, emblemols, and phenolic compounds. (Zhang YJ et al., J Nat Prod, 63: 1507-1510, 2000). Amra contains 20 times more vitamin C than oranges, and it is very stable to heat, so even when exposed to high temperatures for a long time, the vitamins are hardly destroyed like when freshly harvested from trees. It is believed that the vitamin C durability and heat resistance of Amra originate from the tannins contained together. The antioxidant, anti-tumor, and anti-inflammatory activities of Amra fruit extract are known to be influenced by the high content of vitamin C and polyphenols.

The jojoba seed oil is oil extracted by compressing the seeds of a plant called jojoba growing in the desert area, and is an oil extracted in the form of a liquid wax. The jojoba seed oil is well absorbed into the skin and is effective in balancing the skin by delivering moisture to the skin without clogging pores. The jojoba seed oil is good for dry skin or aged skin, and shows excellent moisturizing effect that helps prevent moisture by forming a moisture film on the skin.

In order to prepare the silkworm matured powdered oil, first, a silkworm larvae matured powdered powder and a silkworm pupae matured powdered powder may be prepared.

The silkworm (Bombyx mori L) is a larva of the silkworm moth family (Bombycidae) and is recorded in ancient books such as Donguibogam and Bonchogangmok, and has been used as a folk medicine for malnutrition, pulmonary tuberculosis, pungsa, ikjeong, and stroke. In the past, it has been mainly used only in the jamsa industry, but due to the recent development of the domestic functional food market and life science technology, it is also treated as cosmetics, food, and medical materials.

In addition, silkworms are rich in protein, free amino acids, monounsaturated fatty acids or polyunsaturated fatty acids, minerals, fiber, and deoxynojirimycin, so they have high nutritional value, inhibit free radicals, improve hyperlipidemia, and liver toxicity. Studies on prevention and lowering of blood sugar have been reported.

The silkworm larva mature powder may be used as the silkworm larva mature powder prepared by the following manufacturing method.

First, after preparing the silkworm larvae, the silkworm larvae can be washed.

In the above step, the silkworm larvae may be a larva before becoming a pupa, for example, the silkworm larvae may be a silkworm larvae on the 3rd day of the age of 5, and the washing of the silkworm larvae is performed by washing the prepared silkworm larvae at a temperature of 45 to 50°C. By immersing the prepared silkworm larvae in a 1 to 3 (w/w)% sodium hydrogen carbonate (NaHCO ₃ ) solution maintained at a temperature for 10 to 30 minutes, the surface of the prepared silkworm larva can be washed.

In the above step, the prepared silkworm larvae are washed to remove foreign substances or mulberry leaves remaining on the surface of the silkworm larvae, thereby improving the preference of the produced silkworm mature powder processing oil, and the silkworm mature powder processing oil is easily decayed or denatured by foreign substances. Can be prevented.

Next, the washed silkworm larvae may be first dried.

In the first drying of the washed silkworm larvae in the step, the washed silkworm larvae are dried at a temperature of 45 to 50° C. for 10 to 20 hours to first remove moisture remaining in the washed silkworm larvae.

Then, the first dried silkworm larvae may be second dried with hot air.

In the above step, the first dried silkworm larvae may be performed by applying hot air at a temperature of 135 to 140° C. for 50 to 100 minutes.

Subsequently, the second dried silkworm larvae may be pulverized, powdered, and then aged to prepare a silkworm larva mature powder.

The crushing of the second dried silkworm larvae in the step is powdered by pulverizing the dried silkworm larvae to a particle diameter of 5 to 10 mm, and the maturation of the powdered silkworm larvae causes the powdered silkworm larvae to be 5 to 7 It can be carried out by storing for 20 to 30 hours at a temperature of °C.

The silkworm pupa maturation powder may be used as a silkworm pupa maturation powder prepared by the following manufacturing method.

First, a silkworm pupa can be prepared and washed, and then heated in a bath.

Washing of the silkworm pupa in the step by immersing the silkworm pupae in a 1 to 3 (w/w)% sodium hydrogen carbonate (NaHCO _{3) solution maintained at a temperature of 40 to 45° C. for 20 to 40 minutes,} The surface of the silkworm pupa can be washed, and the heating of the silkworm pupa is performed by sterilizing and disinfecting the washed pupa by immersing the washed pupa in 1 to 1.5% by weight of bamboo saline heated at a temperature of 90 to 95°C. You can keep it clean.

Next, the water remaining in the heated silkworm pupa may be removed by drying the heated silkworm pupa.

In the above step, drying of the heated silkworm pupae may be performed by storing and drying the heated silkworm pupae at a temperature of 70 to 75°C for 20 to 30 hours.

Next, the dried silkworm pupa may be pulverized and powdered.

The pulverization of the dried silkworm pupae in the above step can be pulverized to a particle size of 5 to 10 mm using a known pulverizer.

Subsequently, a mixture may be prepared by mixing the lactic acid bacteria culture solution with the silkworm pupa powder.

In the above step, the lactic acid bacteria culture solution may be mixed in a weight ratio of 10 to 15 parts by weight based on 100 parts by weight of silkworm pupa powder.

In addition, in the step, the lactic acid bacteria may use a lactic acid strain isolated from kimchi, which is a fermented food, specifically, as the lactic acid strain, Lactobacillus curvatus , Weissella viridescens , Lactobacillus plantarum and Any one or more known lactic acid strains selected from the group consisting of Leuconostoc lactis may be used.

More specifically, in the above step, in order to prepare a lactic acid bacteria culture solution using the lactic acid strain isolated from kimchi, the pH of the medium is adjusted to 2.8-3.2 using 1.2N HCl, and then the lactic acid strain is separated from the kimchi, and the separated The lactic acid strain was incubated for 20 to 25 hours at 37 to 39°C using MRS broth (Oxoid, England) and then ^{diluted to 1×1.0 8} to 5×1.0 ⁸ CFU/mL, and then 10 at 10,000 to 15,000 rpm. Centrifugation for ~ 15 minutes to separate only the supernatant, filter the supernatant with a 0.45 μm syringe filter, and then sterilized distilled water to 100 parts by weight of the filtered supernatant, 1,000 to 2,000 parts by weight by weight By mixing and dilution at a ratio, a lactic acid bacteria culture solution can be prepared.

Next, by aging a mixture consisting of the silkworm pupa powder and the lactic acid bacteria culture solution, a silkworm pupa mature powder can be prepared.

In the above step, a mixture consisting of the silkworm pupa powder and the lactic acid bacteria culture solution may be stored for 20 to 30 hours at a temperature of 15 to 17°C and aged for 20 to 30 hours to prepare a silkworm pupa mature powder.

Next, the prepared silkworm larvae maturation powder and the silkworm pupa maturation powder may be mixed to prepare a silkworm maturation powder.

In the above step, the silkworm mature powder may be mixed in a weight ratio of 40 to 60% by weight of the silkworm larva mature powder and 40 to 60% by weight of the silkworm pupa mature powder.

Next, a mixture may be prepared by mixing the mature silkworm powder and avocado oil.

In the above step, it may be mixed in a weight ratio of 1000 to 2000 parts by weight of avocado oil based on 100 parts by weight of the mature silkworm powder.

Subsequently, the mixture of the silkworm aging powder and avocado oil may be heated and then aged.

In the above step, the mixture of the silkworm mature powder and avocado oil is heated at a temperature of 70 to 75°C for 50 to 100 minutes, and then stored and aged for 5 to 10 days at a temperature of 10 to 15°C. It can allow the contained nutrients to be sufficiently leached.

Next, a mixture of the aged silkworm mature powder and avocado oil may be filtered to prepare a silkworm mature powder processed oil.

In the above step, a mixture of the aged silkworm ripening powder and avocado oil can be filtered through a filter to remove solids such as silkworm ripening powder, thereby preparing the silkworm mature powder processing oil. Since it is a technology, a detailed description thereof will be omitted for convenience of description and clarity of the technical idea of the present invention.

The avocado oil can be extracted from avocado, and the avocado oil contains a large amount of unsaturated fatty acids, which greatly reduces the incidence of heart disease, cancer, and diabetes, and also increases the absorption rate of beta-carotene when consumed with green and yellow vegetables. It can be increased by 15.3 times.

The avocado is rich in vitamins and minerals, effectively decomposes cholesterol, can prevent various adult diseases and vascular diseases, is good for a short-term diet, and is rich in lutein and zeagentin, so it is good for eye health.

In addition, the avocado contains important antioxidants such as vitamin C and vitamin E, so it can fight inflammation and alleviate the symptoms of arthritis. It can prevent synthesis.

For example, as the avocado oil, avocado oil prepared by the following manufacturing method may be used.

First, after preparing the avocado, you can wash it thoroughly in water.

Next, the washed avocado may be immersed in grape juice and rancidity inhibitors. As described above, it is possible to prevent discoloration or rancidity of the avocado oil produced by immersing the avocado in grape juice and rancidity inhibitors.

The washed avocado can be immersed in a mixture of grape juice and a rancidity inhibitor in a ratio of 1:1, and the rancidity inhibitor is mixed with vitamin C and a salt solution in a weight ratio of 1:1. Can be manufactured.

The grape juice is good for preventing blood clots and preventing adult diseases such as high blood pressure, arteriosclerosis and heart disease, and can help improve memory, and the salt solution used as the rancidity inhibitor may be a salt solution having a concentration of 1 to 2% by weight, After immersing in a weight ratio of 100 to 120 parts by weight of a mixture of grape juice and rancidity inhibitor with respect to 100 parts by weight of the washed avocado, it may be maintained for 10 to 40 minutes.

Thereafter, the avocado immersed in the mixture of grape juice and rancidity inhibitor may be taken out so that the grape juice and rancidity inhibitor components penetrate into the avocado.

In the above step, by allowing the active ingredient of grape juice and rancidity inhibitor to penetrate to the surface of the avocado, it is possible to prevent discoloration or rancidity of the avocado oil produced even after a long period of time.In this step, in the mixed solution of grape juice and rancidity inhibitor It can be carried out by storing the separated avocado for 60 to 100 minutes at a temperature of 28 to 32 °C.

Subsequently, the avocado into which the components of the grape juice and rancidity inhibitors are infiltrated may be steamed.

The steaming of the avocado can be performed by steaming the avocado into which the components of the grape juice and rancidity inhibitors are infiltrated with steam for 50 to 100 minutes.If the steaming of the avocado is performed below the above lower limit, sufficient steaming is performed. There is a problem that is not achieved, and when it is performed beyond the upper limit range, the difference in effect according to an increase in steaming time may be insignificant.

Next, the steamed avocado can be dried.

Drying of the steamed avocado may be performed for 10 to 20 hours at a temperature of 45 to 50°C of the steamed avocado, and mechanical drying using a dryer may be used to keep the drying temperature of the avocado constant. have.

Next, the dried avocado may be washed with one or more acids of acetic acid or citric acid, and the washing of the dried avocado is performed by washing the surface of the dried avocado with at least one acid of acetic acid or citric acid. There is an effect of improving the flavor and color of avocado oil to be manufactured through a fermentation process.

Subsequently, a fermentation broth consisting of a culture broth and lactic acid bacteria may be sprayed on the washed avocado.

As the lactic acid bacteria, any one known lactic acid bacteria selected from the group consisting of lactic acid bacteria (Lactobacillales ), Streptococcus thermophiles , and Bifidobacterium longum may be used, and the culture medium is peptone , Malt extract, sodium citrate, potassium phosphate dibasic, glucose, and distilled water, and the culture solution is 15 to 25 parts by weight of peptone, malt extract 5 to 15 It may be formed by mixing in a weight ratio of 0.5 to 1.5 parts by weight of sodium citrate, 0.1 to 0.3 parts by weight of dibasic potassium phosphate, 1 to 3 parts by weight of glucose, and 100 to 200 parts by weight of distilled water.

In the culture medium, the peptone is a nitrogen source that supplies protein and supplies proteins necessary for the growth of microorganisms, and the malt extract contains various minerals, B vitamins, and amino acids, which can help the growth of microorganisms, and the sodium citrate Silver is an inorganic substance that maintains an optimized state for the growth of microorganisms by adjusting the pH of the culture medium, and can supply nutrients, and the glucose can function as an important component that lactic acid bacteria metabolize sugar to produce organic acids.

Next, the avocado sprayed with the fermentation broth can be fermented, and the fermentation of the avocado is 3 to 6 days in a storage container maintaining a temperature of 45 to 50°C and a humidity of 60 to 65% of the avocado sprayed with the fermentation broth. Can be stored for fermentation.

Then, the fermented avocado may be dried in cold air.

Cold air drying of the fermented avocado may be performed by drying the fermented avocado with cold air at a temperature of 3 to 6° C. for 5 to 10 hours, and maintaining the fermented avocado at a temperature of 55 to 60° C. for 1 to 3 days.

Subsequently, the cold-air dried avocado is compressed at a humidity of 70 to 80%, a temperature of 120 to 150°C, and a temperature of 20 to 25 kgf/cm ² , and then the oil is separated, thereby preparing avocado oil.

The jade powder functions to improve the moisturizing effect by making the skin smooth and fine, and effectively delivering nutrients to the skin. Jade is divided into jadeite and purgatory, and jadeite is a lead pyroxene mineral belonging to the pyroxene group, and is a monoclinic material composed of silicic acid, aluminum oxide, and soda. The hardness is a dense mass like crystal, and the light is black, cyan, green, transparent or translucent. Usually, jade refers to this jadeite.

Purgatory is a monoclinic fibrous mineral body of inosilicate, and is divided into purgatory and serpentine super-basic purgatory among high-soil marbles. The degree is determined, and it is known that the finer the fiber is, the better the quality.

Hereinafter, examples and comparative examples for skin whitening and moisturizing cosmetic compositions using calcium powder prepared according to the present invention will be described in more detail.

<Example 1>

Abalone, calcium powder using oyster shell 1.5% by weight, Maesengi extract 1% by weight, Bringgraz oil 0.1% by weight, Amra oil 0.1% by weight, Jojoba seed oil 0.03% by weight, Silkworm aging powder processing oil 0.15% by weight, Ox powder 0.005 A cosmetic was prepared by mixing% by weight and the remaining amount of purified water.

<Example 2>

Abalone, Calcium powder using oyster shell 1.1% by weight, Maesengyi extract 1.5% by weight, Bringgraz oil 0.07% by weight, Amra oil 0.14% by weight, Jojoba seed oil 0.02% by weight, Silkworm mature powder processing oil 0.2% by weight, Ox powder 0.002 A cosmetic was prepared by mixing% by weight and the remaining amount of purified water.

<Example 3>

Abalone, calcium powder using oyster shell 2% by weight, Maesengi extract 0.2% by weight, Bringgraz oil 0.14% by weight, Amra oil 0.06% by weight, Jojoba seed oil 0.05% by weight, Silkworm mature powdered end processing oil 0.11% by weight, Jade powder 0.01 A cosmetic was prepared by mixing% by weight and the remaining amount of purified water.

<Comparative Example 1>

A cosmetic was prepared using the same composition as in Example 1, but in Comparative Example 1, a cosmetic was prepared without including abalone, calcium powder using oyster shells, mascarpone extract, and silkworm mature powder processed oil.

<Comparative Example 2>

A cosmetic was prepared using the same composition as in Example 1, but in Comparative Example 2, a cosmetic was prepared without including abalone, calcium powder using oyster shell, bringgraze oil, jojoba seed oil, and silkworm mature powder processing oil.

1. Cosmetic stimulation experiment

The stimulation experiment for the cosmetic prepared as described above was carried out as follows.

First, the clinical trial targeted 20 healthy men and women (7 men and 13 women) between the ages of 20 and 50. After washing the face with soap, cosmetics according to Examples and Comparative Examples were applied without applying cosmetics or moisturizers. After applying to the subject's face and drying, 100 minutes later, skin conditions (erythema, swelling, papules, etc.) were observed, and a qualitative evaluation was performed through an evaluation table as shown in [Table 1].

Experiment item Subjective score

Degree of stimulation a: The skin turns red and the itching is strong b: the skin is slightly red and itchy c: The skin becomes slightly red and there is irritation. d: There is no change in skin condition, but there is a slight irritation. e: No irritation or change in skin condition

The evaluation results of the test subjects for the degree of stimulation in [Table 1] are shown in [Table 2] below.

Experiment item Subjective score Evaluation results Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Degree of stimulation a - - - - - b - - - - - c - - - - - d - - - - - e 20 20 20 20 20

As can be seen from the above [Table 2], it was confirmed that the cosmetic composition using the composition according to the Examples and Comparative Examples had little irritation or change in skin condition, and was a safe substance with little irritation such as erythema and swelling.

2. Formulation stability test

The degree of stabilization of the formulation of the cosmetic prepared according to Examples 1, 2, and 3 was evaluated.

The evaluation of the degree of stabilization of the formulation was carried out by checking the phase stability by storing the cosmetic samples prepared according to Examples 1, 2, and 3 for 25 days at a temperature of 40°C, room temperature, and -20°C, respectively, and the results are as follows. It is shown in [Table 3] of.

division Example 1 Example 2 Example 3 40℃ Good Good Good Room temperature Good Good Good -20℃ Good Good Good

Referring to [Table 3], it was confirmed that the cosmetic compositions prepared according to Examples 1, 2, and 3 had good formulation stability.

3. Antibacterial activity evaluation experiment

In order to evaluate the antibacterial activity of the cosmetics prepared according to Example 1 and Comparative Example 1, the following experiment was conducted. In the antibacterial activity evaluation experiment, propionibacterium acnes ATCC 6919, which is an acne bacteria, was used as a test bacterium.

After inoculating acne bacteria into each BHI broth, pre-culture, inoculate 100 ml of sterilized new BHI broth containing 5% of each composition at a concentration of 1%, incubate in an anaerobic incubator for 2 days, and then measure the number of bacteria. I did.

division P.acnes Inoculum concentration (cfu/ml) Bacterial concentration after cultivation (cfu/ml) Example 1 2.45 x 10 ⁶ 2.21 x 10 ³ Comparative Example 1 2.45 x 10 ⁶ 5.51 x 10 ⁵

Referring to [Table 4], it can be seen that the cosmetic composition prepared according to Example 1 significantly improved antibacterial activity against acne bacteria.

4. Skin moisturizing and skin tone improvement experiment

In a survey conducted after applying the cosmetic composition prepared according to Example 1 to the face for 4 weeks once a day for 15 women aged 20 to 50 years, skin moisturizing and skin tone improvement were evaluated. The results are shown in the following [Table 5].

The evaluation was evaluated on a 5-point scale with a scale of 1 to 5, and the higher the score, the better.

division Before use after use Moisturize the skin 2.2 4.3 Skin elasticity 2.3 2.9 Skin wrinkles 2.2 2.8 Skin blemishes, pigmentation 2.1 2.7 Skin tone 2.3 4.0 pore 2.1 2.6

Referring to [Table 5], in a survey conducted after applying the cosmetic composition prepared according to Example 1 to the face once a day for 4 weeks, 11 of the experimenters showed improvement in skin moisturization and skin tone. They responded that it was improved, and 6 of them responded that it improved significantly.

5. Moisturizing effect measurement

A capacitance measurement method using Corneometer CM820 (German Courage) was used, and the moisturizing effect was compared and measured using Example 1 and Comparative Example 1 as a sample.

15 women aged 30 to 50 were subject to adaptation to the inner side of the lower arm just before the start of the test for 20 minutes in a constant temperature and humidity room, and then the skin moisture was kept constant. Then, after measuring the initial water retention amount of each sample application area using Corneometa C820, the cosmetics prepared according to Example 1 and Comparative Example 1 were applied.

The moisture increase/decrease rate was measured first 20 minutes after application, and 2 hours later, the moisture increase/decrease rate was measured again. Each measurement site was repeatedly measured 3 times, and the skin moisture increase/decrease rate was calculated according to the following [Equation 1] as the average value and shown in [Table 6].

[Equation 1]

Water increase/decrease rate (%) = (Tdi-Tdo)-(Ntdi-NTdo) / ((Ntdi-Ntdo) + Tdo) * 100

Tdi: nth measured value of the sample application site

Tdo: Measured value before sample application

Ntdi: The nth measured value of the area where the sample was not applied

NTdo: Measured value before application of the area where the sample was not applied

division Example 1 Comparative Example 1 20 minutes 32.89 8.82 120 minutes 20.34 4.76

Referring to [Table 6], it was confirmed that the cosmetic composition prepared as in Example 1 had better moisturizing effect than the cosmetic composition of Comparative Example 1 at the initial stage as well as over time.

6. Visual evaluation of whitening improvement

Visual evaluation of the improvement of whitening was performed on the cosmetic prepared according to Example 1.

1A and 1B are photographs showing changes in state before applying the cosmetic prepared according to Example 1 to a 29-year-old woman (Fig. 1A) and after applying it for 2 months (Fig. 1B), and Figs. 2A and 1B. 2b is a photograph showing the state change before applying the cosmetic prepared according to Example 1 to a 33-year-old woman (FIG. 2A) and after applying it for 2 months (FIG. 2B), and FIGS. 3A and 3B are examples. It is a photograph showing the state change before applying the cosmetic prepared according to 1 to a 38-year-old woman (FIG. 3A) and after applying it for 2 months (FIG. 3B).

Referring to FIGS. 1A to 3B, it was confirmed that the cosmetic composition prepared according to Example 1 became clear and clean after use.

In the above, a preferred embodiment of the present invention has been described, but those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in other specific forms without changing the technical spirit or essential features. I will be able to. Therefore, it should be understood that the exemplary embodiment described above is illustrative in all respects and is not limiting.

Claims (5)

Abalone, 1 to 2 parts by weight of calcium powder using oyster shells, 0.1 to 1.5 parts by weight of Maesengi extract, 0.05 to 0.15 parts by weight of Bringgraz oil, 0.05 to 0.15 parts by weight of Amra oil, 0.01 to 0.05 parts by weight of jojoba seed oil, silkworm It is contained in a weight ratio of 0.1 to 0.2 parts by weight of aging powder processing oil and 0.001 to 0.01 parts by weight of jade powder,
Calcium powder using the abalone and oyster shell,
After preparing the abalone shell and oyster shell, wash them to remove foreign substances adhering to the abalone shell and oyster shell,
The washed abalone shell and oyster shell are first pulverized to prepare a pulverized abalone shell and oyster shell assembly,
The abalone shell and oyster shell granulated pulverized product is immersed in the mixed solution,
The abalone shell and oyster shell granulated pulverized product are separated from the mixed solution and taken out, and the abalone shell and oyster shell granulated pulverized product are heated and fired,
The calcined abalone shell and oyster shell granulated pulverized product is second pulverized and impurities are removed to prepare an abalone shell and oyster shell granulated pulverized product,
The abalone shell and oyster shell fine-grained pulverized product is inoculated by spraying mineral and lactic acid bacteria culture solution,
Fermenting the abalone shell and oyster shell fine pulverized product inoculated with the mineral and lactic acid bacteria culture solution,
The fermented abalone shell and oyster shell fine pulverized product is washed, dried, and sterilized to prepare calcium powder,
In the step of immersing the abalone shell and oyster shell granulated pulverized product in the mixed solution, the mixed solution is 0.1 to 0.5% by weight of nitric acid, 0.5 to 1.5% by weight of phosphate compound, 0.01 to 0.1% by weight of persulfate, 1 to 3% by weight of acetic acid and the balance It is made of purified water of, and is performed by immersing the abalone shell and oyster shell granulated pulverized product in the mixed solution for 10 to 30 minutes, and the phosphate compound is a first sodium phosphate (NaH ₂ PO ₄ ), a second sodium phosphate ( Na ₂ HPO ₄ ), tertiary sodium phosphate (Na ₃ PO ₄ ), first potassium phosphate (KH ₂ PO ₄ ), second potassium phosphate (K ₂ HPO ₄ ), first ammonium phosphate ((NH ₄ )H ₂ PO ₄ ), second ammonium phosphate ((NH ₄ ) ₂ HPO ₄ ) and tertiary ammonium phosphate ((NH ₄ ) ₃ PO ₄ ) are used, and as the persulfate, persulfate Any one or more selected from ammonium ((NH ₄ ) ₂ S ₂ O ₈ ), sodium persulfate (Na ₂ S ₂ O ₈ ) or potassium persulfate (K ₂ O ₈ S _{2) is used,}
After separating and removing the abalone shell and oyster shell granulated pulverized product from the mixed solution, heating and sintering the abalone shell and oyster shell granulated pulverized product is 1,300 after putting the separated abalone shell and oyster shell granulated pulverized product into a sintering furnace. It is carried out by heating for 30 to 200 minutes at a temperature of to 1,600 °C,
In the step of spraying and inoculating the abalone shell and oyster shell fine pulverized product with minerals and lactic acid bacteria culture solution, the weight of 1 to 5 parts by weight of mineral and 5 to 10 parts by weight of the lactic acid bacteria culture solution based on 100 parts by weight of the abalone shell and oyster shell fine pulverized product Inoculate by spraying at a ratio, and the mineral is one or more selected from the group consisting of calcium, phosphorus, potassium, magnesium, manganese, iodine, selenium, molybdenum and cobalt, and the lactic acid bacteria culture medium is a medium using 1.2N HCl. After adjusting the pH of 2.8 to 3.2, separating the lactic acid strain from kimchi, and incubating the isolated lactic acid strain at 37 to 39°C for 20 to 25 hours using MRS broth (Oxoid, England) and then 1×1.0 ^{Dilute to 8} ~ 5×1.0 ⁸ CFU/mL, and then centrifuge at 10,000 to 15,000 rpm for 10 to 15 minutes to separate only the supernatant, and use the supernatant with a 0.45 μm syringe filter. After filtration, it is prepared by mixing and diluting at a weight ratio of 1,000 to 2,000 parts by weight of sterilized distilled water with respect to 100 parts by weight of the filtered supernatant, and the lactic acid strain is Lactobacillus curvatus (Lactobacillus curvatus) , Vicella viridesense ( Weissella viridescens ) , Lactobacillus plantarum and Any one or more lactic acid strains selected from the group consisting of Leuconostoc lactis are used,
In the step of fermenting the pulverized abalone shell and oyster shell fine particles inoculated with the mineral and lactic acid bacteria culture medium, the fine pulverized abalone shell and oyster shell fine pulverized products inoculated with the mineral and lactic acid bacteria culture medium are prepared at a temperature of 40 to 45°C and 60 to 65%. After allowing the humidity to be maintained, it is fermented for 3 to 7 days,
The Maesengi extract,
After preparing Maesamyeongi, the first washing is performed with purified water at a temperature of 15 to 25°C, and the second washing is performed by immersing the first washed Maesaengi in an aging solution, and the second washing is 0.5:1.5 of green tea leaves, bay leaves and purified water. Mixing at a weight ratio of :8, and after heating the purified water in which the green tea leaves and laurel leaves are mixed at a temperature of 70 to 75°C for 20 to 60 minutes, the green tea leaves and bay leaves are removed and filtered to prepare a filtrate. , Squid ink is mixed in the filtrate at a weight ratio of 9.5:0.5 and aged at a temperature of 10 to 15° C. for 3 to 6 hours to prepare an aging liquid, and after immersing the maesangyi washed with the purified water in the aging liquid It is stored for 10 to 20 minutes at a temperature of 5 to 7°C, and after separating the Maesaeans immersed in the aging solution, washing with purified water at a temperature of 15 to 20°C is performed. Stored for 20 to 40 hours at a temperature of 50° C., dried, and mixed in a weight ratio of 1 to 5 parts by weight of dermal fermentation broth with respect to 100 parts by weight of the dried meeseng, and then stored for 20 to 40 hours at a temperature of 8 to 12° C. By doing so, the dermis fermentation is aged and fermented, and the dermal fermentation broth is washed after preparing the dermis, and the dermis is enzymatically decomposed, but with the enzymes alpha-amylase, pectinase, and xylanase. ) And cellulase are used, and with respect to the total 100 parts by weight of the dermis, 0.01 to 0.03 parts by weight of the alpha amylase, 0.02 to 0.04 parts by weight of pectinase, Xylanase ) 0.03 to 0.05 parts by weight and 0.02 to 0.04 parts by weight of cellulase, and by storing the dermis mixed with the enzyme at a temperature of 38 to 42° C. for 10 to 50 hours, enzymatic decomposition is performed, The enzymatically decomposed dermis is kept at a temperature of 8 to 12° C. for 20 to 40 days. It is not stored and aged, and the aged dermis is filtered using a filter to prepare a filtered dermal liquid, and the filtered dermal liquid is concentrated and sterilized to prepare a dermal concentrate, and based on 100 parts by weight of the dermal concentrate After mixing in a weight ratio of 0.1 to 0.2 parts by weight of vegetable lactic acid bacteria, it is prepared through a process of fermenting for 3 to 6 days at a temperature of 10 to 15°C, and freezing the aged fermented Maesaeung at a temperature of -20 to -30°C After drying and pulverizing the freeze-dried Maesengi powder to prepare Maesengi powder, mixed in a weight ratio of 2,000 to 2,500 parts by weight of purified water with respect to 100 parts by weight of Maesengi powder, and for 5 to 10 hours at a temperature of 90 to 95°C. Extracted to prepare a hot water extract of Maesengi, the hot water extract of Maesengi is freeze-dried at a temperature of -40 to -50°C, and the freeze-dried Maeseng is prepared by reflux extraction of the hot water extract,
The silkworm ripening powder processed oil,
Prepare silkworm larva mature powder and silkworm pupa mature powder, but the silkworm larva mature powder is 1 to 3 (w/w)% hydrogen carbonate that maintains the silkworm larva at a temperature of 45 to 50°C after preparing the silkworm larva. The prepared silkworm larvae are immersed in sodium (NaHCO ₃ ) solution for 10 to 30 minutes to wash, and the washed silkworm larvae are first dried for 10 to 20 hours at a temperature of 45 to 50°C, and the first dried After the second drying of the silkworm larvae in a hot air at a temperature of 135 to 140°C for 50 to 100 minutes, the second dried silkworm larvae are pulverized to a particle diameter of 5 to 10 mm and powdered at a temperature of 5 to 7°C. It is prepared through a process of storing and aging for 30 hours, and the washed pupa is prepared and washed with the washed pupa in 1 to 1.5% by weight bamboo salt water heated at a temperature of 90 to 95°C. The silkworm pupae is heated by immersion, and the silkworm pupae heated in the middle bath is stored and dried for 20 to 30 hours at a temperature of 70 to 75°C, and the dried silkworm pupae is pulverized and powdered, and 100 parts by weight of the silkworm pupae powder To prepare a mixture by mixing in a weight ratio of 10 to 15 parts by weight of the lactic acid bacteria culture solution, the lactic acid bacteria culture solution is adjusted to the pH of the medium to 2.8 ~ 3.2 using 1.2N HCl, and then separate the lactic acid strain from kimchi, and the separated The lactic acid strain was incubated for 20 to 25 hours at 37 to 39°C using MRS broth (Oxoid, England) and then ^{diluted to 1×1.0 8} to 5×1.0 ⁸ CFU/mL, and then 10 at 10,000 to 15,000 rpm. Centrifugation for ~ 15 minutes to separate only the supernatant, filter the supernatant with a 0.45 μm syringe filter, and then sterilized distilled water to 100 parts by weight of the filtered supernatant, 1,000 to 2,000 parts by weight by weight Diluting by mixing in proportions It is prepared through a process, and prepared through a process of storing and aging a mixture consisting of the silkworm pupa powder and lactic acid bacteria culture solution at a temperature of 15 to 17°C for 20 to 30 hours,
The prepared silkworm larva mature powder is mixed in a weight ratio of 40 to 60% by weight of the prepared silkworm larva mature powder and 40 to 60% by weight of the silkworm pupa mature powder to prepare a silkworm mature powder,
A mixture is prepared by mixing in a weight ratio of 1000 to 2000 parts by weight of avocado oil based on 100 parts by weight of the silkworm ripening powder,
The mixture of the silkworm aging powder and avocado oil is heated at a temperature of 70 to 75°C for 50 to 100 minutes, and then stored and aged at a temperature of 10 to 15°C for 5 to 10 days,
It is prepared through a process of filtering a mixture of the aged silkworm mature powder and avocado oil,
The avocado oil,
After preparing the avocado, the washed avocado is immersed in a mixture of grape juice and a rancidity inhibitor in a weight ratio of 1:1, but the rancidity inhibitor is mixed with vitamin C and a salt solution in a weight ratio of 1:1. Avocados prepared and immersed in a weight ratio of 100 to 120 parts by weight of a mixture of grape juice and rancidity inhibitor with respect to 100 parts by weight of the washed avocado and maintained for 10 to 40 minutes, and immersed in the mixture of grape juice and rancidity inhibitors Take out and store for 60 to 100 minutes at a temperature of 28 to 32°C to allow the components of grape juice and rancidity inhibitor to penetrate into the avocado, and the avocado into which the ingredients of grape juice and rancidity inhibitor have penetrated with water vapor for 50 to 100 minutes The steamed avocado is steamed for 10 to 20 hours at a temperature of 45 to 50°C, and the dried avocado is washed with at least one acid of acetic acid or citric acid, and a culture solution and A fermentation broth consisting of lactic acid bacteria is sprayed, but any one lactic acid bacteria selected from the group consisting of lactic acid bacteria (Lactobacillales ), Streptococcus thermophiles , and Bifidobacterium longum is used as the lactic acid bacteria, The culture solution includes 15 to 25 parts by weight of peptone, 5 to 15 parts by weight of malt extract, 0.5 to 1.5 parts by weight of sodium citrate, 0.1 to 0.3 parts by weight of dibasic potassium phosphate, 1 to 3 parts by weight of glucose, and 100 to 200 parts by weight of distilled water. It is made by mixing in a ratio, and fermented by storing the avocado sprayed with the fermentation broth for 3 to 6 days in a storage container maintaining a temperature of 45 to 50 °C and a humidity of 60 to 65%, and the fermented avocado is 3 to Dry with cold air at a temperature of 6° C. for 5 to 10 hours, and dry with cold air by holding at a temperature of 55 to 60° C. for 1 to 3 days. , Skin whitening using calcium powder, characterized in that the cold-air dried avocado is compressed at a humidity of 70 to 80%, a temperature of 120 to 150°C, and a temperature of 20 to 25 kgf/cm ^{2 and then separating the oil.} Cosmetic composition for moisturizing. delete delete delete delete

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