CN115645349A - Composition with effects of moisturizing, repairing and restoring skin homeostasis and preparation method and application thereof - Google Patents
Composition with effects of moisturizing, repairing and restoring skin homeostasis and preparation method and application thereof Download PDFInfo
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- CN115645349A CN115645349A CN202211089449.0A CN202211089449A CN115645349A CN 115645349 A CN115645349 A CN 115645349A CN 202211089449 A CN202211089449 A CN 202211089449A CN 115645349 A CN115645349 A CN 115645349A
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Abstract
The invention belongs to the technical field of cosmetics, and discloses a composition with the effects of moisturizing, repairing and restoring skin homeostasis, and a preparation method and application thereof. The composition with the effects of moisturizing, repairing and recovering the skin stability comprises red yeast rice fermentation liquid and lactobacillus fermentation lysate, wherein the mass ratio of the red yeast rice fermentation liquid to the lactobacillus fermentation lysate is (1-15): 1-10. The composition provided by the invention has the effects of moisturizing, brightening, repairing skin barriers and restoring skin microecological balance, especially aims at the skin barrier damage caused by acid brushing or excessive cleaning, skin acid-base environment imbalance and skin surface microorganism imbalance, has a synergistic effect, pays more attention to the metabolism process of the skin, quickly establishes a new microorganism group in the process, and brightens the skin and helps the skin restore the steady state by improving the external environments such as pH value and humidity for the existence of the microorganism group.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a composition with effects of moisturizing, repairing and restoring skin homeostasis and a preparation method and application thereof.
Background
The epidermis is the most active part of the human body, and in healthy skin, about 14% of the basal layer keratinocytes are in cell division at any time, undergo cell division, differentiation, and move upward, in the process of which they become part of the stratum corneum. Although the stratum corneum is a powerful and effective physical barrier, it is constantly challenged by fluctuating ambient temperature and humidity, ultraviolet light, microorganisms, daily hygiene (water, surfactants), foreign molecules, and the like. In order to maintain this vital function of the physical barrier, the epidermis must constantly renew itself, making it the most active part of the human body. If the epidermal renewal process is successful, the skin will have optimal barrier function, hydration and aesthetics.
Many microorganisms live on the skin, and many resident skin microorganisms interact with the skin. Various molecules produced by microorganisms interact with skin cells (e.g., differentiating keratinocytes) which respond to benefit the skin, while the keratinocytes produce molecules beneficial to "healthy" microorganisms. For example, molecules that modulate the pH of the skin are produced, as well as antimicrobial peptides that can kill competing species of microorganisms. The action between the skin and the beneficial, mutually beneficial microorganisms is bidirectional, with both parties in a dynamically balanced symbiotic relationship.
Billions of skin microorganisms are killed, washed off, rubbed off, or shed with dead skin cells each day. Nevertheless, there are still a large number of microorganisms on the skin and their composition is surprisingly stable, since the skin microflora also renews itself like the epidermis. Any loss that takes place on the skin surface is remedied by the microorganism renewal process, and the state of epidermis is closely relevant with skin surface microecological environment, and dry skin stratum corneum is thick, is difficult for droing, consequently is unfavorable for metabolic going on, and moist skin environment is favorable to going on of epidermis normal metabolism. Thus, the moist environment is able to maintain the normal metabolism of the skin, thereby promoting a relatively rapid access of the "new microbiome" to the skin surface. The pH, humidity, temperature, supply of food needed, etc. of the skin can also make the environment more favorable to these microorganisms, and a healthy, balanced skin microflora can interact with skin cells (e.g., differentiating keratinocytes) to ultimately benefit the skin. However, in the existing micro-ecological friendly skin care products, the balance of micro-ecological environment is achieved only aiming at the promotion or inhibition of a certain bacterium or a certain class of bacteria.
Disclosure of Invention
The invention aims to overcome the defects of the background technology and provide a composition with the effects of moisturizing, repairing and restoring skin homeostasis, and a preparation method and application thereof. The compositions of the invention are more concerned with the metabolic processes of the skin itself and the rapid establishment of a "new microbiome" in this process. And helps the skin to restore the steady state by improving the external environments such as pH value, humidity and the like for the survival of the microorganism group.
In order to achieve the purpose of the invention, the composition with the effects of moisturizing, repairing and restoring skin stability comprises red yeast rice fermentation liquor and lactobacillus fermentation lysate.
Further, in some embodiments of the invention, the mass ratio of the red yeast rice fermentation broth to the lactic acid bacteria fermentation lysate is 1-15:1-10.
Preferably, in some embodiments of the present invention, the mass ratio of the red yeast rice fermentation broth to the lactic acid bacteria fermentation lysate is 1-10:1-3.
More preferably, in some embodiments of the present invention, the mass ratio of the red yeast rice fermentation broth to the lactic acid bacteria fermentation lysate is 3-4:1.
further, in some embodiments of the present invention, the preparation method of the red yeast rice fermentation broth comprises: culturing the monascus strain on a slant culture medium, washing spores with sterile water, filtering, oscillating to prepare uniform spore suspension, inoculating the spore suspension into a liquid culture medium, and performing shake culture to obtain a liquid seed solution; cleaning rice, draining, crushing, adding water, stirring, gelatinizing, cooling to room temperature, inoculating monascus liquid seed liquid, stirring and fermenting at 25-45 ℃, and performing spray drying after completion to obtain monascus fermentation products; adding deionized water into the obtained monascus fermentation product, inoculating lactobacillus, stirring and fermenting; after fermentation, sterilizing and centrifuging to obtain supernatant, namely the red yeast fermentation liquid.
Further, in some embodiments of the invention, the method of preparing the lactic acid bacteria fermentation lysate comprises: taking lactobacillus strains, carrying out streak constant-temperature culture in a slant culture medium, washing the strains, and centrifuging to obtain lactobacillus suspension; inoculating the lactobacillus suspension into a liquid culture medium, performing activated seed culture to obtain lactobacillus seed liquid, inoculating into a fermentation tank, stirring, ventilating, and culturing until fermentation is finished; filtering to obtain thallus, and breaking cell wall to obtain lactobacillus fermentation lysate.
Further, in some embodiments of the present invention, a stabilizer is also included in the composition.
Preferably, in some embodiments of the invention, the stabilizer is one or more of glycerol, ethylhexylglycerol, 1, 2-hexanediol, pentanediol.
More preferably, in some embodiments of the invention, the ratio of the mass of the red yeast rice fermentation broth and the lactic acid bacteria fermentation lysate to the stabilizer is 0.3-5:1.
in another aspect, the present invention also provides a preparation method of the composition with the effects of moisturizing, repairing and restoring skin homeostasis, wherein the method comprises the following steps:
1) Preparing a seed solution: culturing an monascus strain on a slant culture medium, washing spores with sterile water, filtering, oscillating to prepare uniform spore suspension, inoculating the spore suspension into a liquid culture medium with the inoculation amount of 1-10%, and culturing for 1-3d by a shaking table to obtain a liquid seed solution;
2) Washing rice, draining, crushing, adding water and stirring, wherein the water-material ratio is controlled to be 8-12:1, gelatinizing, cooling to room temperature, and inoculating monascus liquid seed solution with the inoculation amount of 4-10%;
3) Fermenting at 25-45 deg.C for 2.5-3.5 days, stirring, fermenting, and spray drying to obtain Monascus fermentation product;
4) Adding 25-35 times of deionized water into the obtained monascus fermentation product, inoculating 8-12% of lactobacillus, stirring and fermenting;
5) After fermentation, sterilizing and centrifuging to obtain supernatant fluid, namely red yeast fermentation liquor;
6) Taking lactobacillus strains to perform streak culture in a slant culture medium, washing the strains after constant-temperature culture, and centrifuging to obtain lactobacillus suspension;
7) Inoculating the lactobacillus suspension into a liquid culture medium, and performing activated seed culture;
8) Inoculating the lactobacillus seed solution into a fermentation tank, stirring, ventilating, and culturing until the fermentation is finished;
9) Filtering to obtain thallus, and breaking the wall to obtain lactobacillus fermentation lysate;
10 Mixing the obtained lactobacillus fermentation lysate into the obtained red yeast fermentation liquid, stirring and mixing uniformly, adding a stabilizer and an additive, mixing, and filtering to obtain the lactic acid bacteria fermented red yeast rice.
Further, in some embodiments of the invention, the spore suspension concentration in step 1) is 1 × 10 5 -1×10 7 CFU/mL。
Further, in some embodiments of the present invention, the stirring rate in step 8) is 100 to 300r/min, and the aeration rate is 0.3 to 0.6L/min.
Preferably, in some embodiments of the present invention, the stabilizer is one or more of glycerol, ethylhexylglycerol, 1, 2-hexanediol, pentanediol.
More preferably, in some embodiments of the invention, the ratio of the mass of the red yeast rice fermentation broth and the lactic acid bacteria fermentation lysate to the stabilizer is 0.3-5:1.
in still another aspect, the invention also provides an application of the composition with the effects of moisturizing, repairing and restoring skin homeostasis, wherein the application is to add the composition into cosmetics.
Further, in some embodiments of the present invention, the cosmetic is a lotion, emulsion, spray, cream, mask, foundation, but those skilled in the art should appreciate that these are merely examples, and the cosmetic of the present invention includes, but is not limited to, lotion, emulsion, spray, cream, mask, foundation.
Compared with the prior art, the invention has the following advantages:
(1) Compared with the traditional extraction mode, the double fermentation method can weaken the color of the red yeast rice, improve the stability of the red yeast rice, simultaneously avoid the loss of active substances in the red yeast rice by the traditional extraction process, release nutrient substances beneficial to the growth of beneficial bacteria after the lactobacillus is subjected to ultrasonic pyrolysis, and reduce the introduction of components irritating the skin by the ultrasonic and high-pressure homogeneous wall breaking mode.
(2) The combination of monascus and lactobacillus in the composition can release more small-molecule active substances, is convenient for skin absorption and utilization, improves moisturizing effect, and recovers stable skin environment more quickly.
(3) The combination of the lactic acid bacteria and the red yeast rice leavening can realize synergistic interaction, not only can balance the skin micro-ecology, but also creates a living environment suitable for the skin microbiome.
(4) Compared with the common microecological regulation composition, the lactic acid bacteria and red yeast rice fermentation composition has the advantages that the skin metabolism is promoted, the differentiation and regeneration effects of the epidermis are improved, the rapid establishment of a new microbial group of the skin is promoted through the differentiation and regeneration of the epidermis, and the composition is excellent in moisturizing, skin barrier function recovery and brightening.
Drawings
FIG. 1 is a (%) showing recovery of the abundance of microorganisms after using the compositions of examples 6 and 9 of the present invention;
FIG. 2 shows the recovery (%) of the microbial diversity after the use of the compositions of examples 6 and 9 of the present invention;
FIG. 3 is a graph showing the reduction (%) of the epidermal water loss value of Tewl values after using the compositions of examples 6 and 9 of the present invention;
figure 4 is a graph of skin texture before and after use of the compositions of examples 6 and 9 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention. It is to be understood that the following description is only illustrative of the present invention and is not to be construed as limiting the present invention.
As used herein, the terms "comprises," "comprising," "includes," "including," "has," "having," "contains" or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when the range "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise specified, the range is intended to include the endpoints thereof, and all integers and fractions within the range.
The indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the number clearly indicates the singular.
Furthermore, the description below of the terms "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily for the same embodiment or example. Further, the technical features of the embodiments of the present invention may be combined with each other as long as they do not conflict with each other.
Example 1
A preparation method of the composition with the effects of moisturizing, repairing and restoring skin homeostasis comprises the following steps:
(1) Culturing Monascus strain on slant culture medium, washing spores with sterile water, filtering, transferring into sterile triangular flask with glass beads, and shaking to obtain uniform spore suspension with concentration of 1 × 10 5 -1×10 7 CFU/mL, inoculating the spore suspension into a liquid culture medium in an inoculation amount of 4%, and performing shake culture for 3d to obtain a liquid seed solution;
(2) Washing rice, draining, crushing, adding water, stirring, and controlling the water-material ratio to be 10:1, inoculating the seed liquid obtained in the step (1) with an inoculation amount of 10%;
(3) After the step (2) is finished, stirring and fermenting for 3d at the temperature of 30 ℃, and after the fermentation is finished, carrying out spray drying to obtain a monascus fermentation product;
(4) Adding 30 times of deionized water into the monascus fermentation product obtained in the step (3), inoculating 10% of lactobacillus, and stirring for fermentation;
(5) After fermentation, sterilizing and centrifuging to obtain supernatant;
(6) Taking the strain, streaking in a slant culture medium, culturing at constant temperature, washing the strain, centrifuging to obtain lactobacillus suspension with the concentration of 1 × 10 6 -1×10 8 CFU/mL CFU/mL；
(7) Inoculating the bacterial suspension into a liquid culture medium, and performing activated seed culture for 24h;
(8) Inoculating lactobacillus seed solution into a fermentation tank at an inoculation amount of 10%, stirring at a speed of 100-300r/min, ventilating for 0.5L/min, and culturing until fermentation is finished;
(9) Filtering to obtain thallus, and breaking cell wall to obtain lactobacillus fermentation lysate;
(10) Mixing the lactobacillus fermentation lysate obtained in the step (9) into the supernatant obtained in the step (5) according to a ratio, stirring and uniformly mixing, adding a stabilizer and an additive, mixing, and filtering to obtain the lactobacillus fermentation lysate;
(11) Wherein the step (4) is the step of inoculating 10% of lactobacillus as a comparative example 1-1, and the step (4) is the step of inoculating 10% of lactobacillus as a comparative example 1-2, and the rest of the preparation method is as described above.
TABLE 1 compositional proportions (by mass) of each composition in examples 1 to 10 and comparative examples 1 to 2
Compositions of each example and comparative example were prepared according to table 1 and by the above-described method, and the resulting compositions were subjected to efficacy tests.
Cytotoxicity evaluation test:
MTT assay is an experimental method widely used for detecting cytotoxicity or cell proliferation by measuring the number of living cells, and the detection principle is that living cells are aliveSuccinate dehydrogenase in mitochondria of cells can enable water-soluble yellow salt MTT (3- (4, 5-dimethylthiazole-2) -2, 5-diphenyl tetrazolium bromide) to be reduced into water-insoluble blue formazan and be deposited in cells, but dead cells do not have the function. The generated crystalline formazan is generally added with DMSO (dimethyl sulfoxide), and the absorbance is measured after dissolution. The specific experimental method is 1X10 in 96-well plate 4 Each of the cells was inoculated at a density of 100. Mu.l each with DMEM medium containing 10% bovine serum and keratinocytes (HaCaT), and the medium was changed to serum-free medium after 24 hours of culture. The culture was carried out for 24 hours after the treatment by adding the compositions at different concentrations to serum-free medium, respectively. Thereafter, the medium was removed, treated with 20. Mu.l of MTT solution, and allowed to react at 37 ℃ for 2 hours. 200 μ l of isopropanol was added to the cells from which the MTT solution was removed, gently shaken for 30min to completely dissolve the crystalline formazan, the absorbance was measured at 570nm, and the cell viability was calculated according to the following formula.
The blank was tested without the addition of composition.
TABLE 2 cytotoxicity results of the compositions obtained in examples 1 to 10 and comparative examples 1 to 1, 1 to 2, 2
Keratinocyte gene expression assay:
filaggrin (FLG) is an important condition for the terminal differentiation of keratinocytes, plays an important role in the differentiation and formation of epidermis, and can be used as an index for evaluating the barrier function and the differentiation state of the epidermis.
The regulation of the composition on the target gene expression level is detected by adopting a real-time fluorescent quantitative PCR method, and the samples to be detected are as follows: examples 1 to 10, comparative examples 1 to 1, 1 to 2, comparative example 2, concentrations: 1 percent. HaCaT cells with 3 x10 5 And inoculating the cells to a 6-well plate, culturing for 24h, and carrying out adherent growth on the cells. The test sample was prepared at 1X, 2ml per well was added to the cell plate, and the assay was 5% CO at 37 ℃ 2 After the constant-temperature incubator is continuously incubated for 24 hours, cells are collected, total RNA of the cells is extracted by using a PureLink mini RNA extraction kit, the concentration of nucleic acid is quantitative, 200-800ng of RNA in each tube is used as a template, and the relative expression quantity (20 mu l system) of each gene is detected by performing Real-Time quantitative fluorescence PCR (reverse transcription + Real Time-PCR) reaction in a Taqman one-step method.
Calculating the formula: fold change =2 -ΔΔCT ；
- Δ Δ CT = [ (CT target gene-CT reference gene) treated sample- (CT target gene-CT reference gene) untreated sample ]. In this experiment, 1 target gene was FLG and 1 reference gene was GAPDH.
TABLE 3 results of the measurement of the regulation of the expression level of the target Gene by the composition
From the above table, it can be seen that:
1) The FLG gene expression levels of the compositions obtained in the examples 1 to 10 are higher than those of the comparative examples, which shows that the red yeast rice fermentation broth and the lactobacillus fermentation lysate obtained in the invention can obtain better FLG gene expression effect in a combined mode, and in addition, compared with the comparative examples 1 to 2, the comparative examples 1 to 1 promote the FLG gene expression to be obviously improved, which shows that the fermentation mode of monascus and lactobacillus is more advantageous;
2) The expression of FLG gene and the obtained red yeast fermentation liquid and lactobacillus fermentation lysate have a certain quantitative effect relationship, and the rising trend is slowed down after the mass fraction is increased to a certain range. Through comparative examples 1,2, 3, 6, 8 and 9, it is found that the FLG gene expression differences of 1, 8 and 15 parts by mass of the red yeast rice fermentation liquor are small, and the FLG gene expression is greatly improved when the mass parts by mass of the red yeast rice fermentation liquor are 2, 3 and 4; the comparison of examples 6 and 7 and examples 9 and 10 shows that the FLG gene expression of the lactobacillus fermentation lysate with the mass parts of 1 and 5 is not greatly different; the FLG gene expression levels were highest in examples 6 and 9. Therefore, example 6 (4) and example 9 (3.
In conclusion, the mass parts of the red yeast rice fermentation liquid are different in the ability of promoting FLG gene expression corresponding to different proportion combinations, and the mass ratio of the red yeast rice fermentation liquid to the lactobacillus fermentation lysate is 1-10:1-3, and more preferably, the mass ratio of the red yeast rice fermentation liquid to the lactobacillus fermentation lysate is 4:1 and 3:1.
and (3) safety patch testing:
dripping 20 mul of solution to be detected into a spot tester, wherein a control hole is a blank control (pure water); the spot tester with the tested object is attached to the bent side of the forearm of the tested person, and the tested person is lightly pressed by the palm to be uniformly attached to the skin for 24 hours; the skin irritation and sensitization were observed as in table 4 after 30min, 24 hours, and 48 hours, respectively, after removal of the test article plaque test device, and the observation results were recorded.
TABLE 4 skin reaction grading Standard for skin Enclosed Patch test
The experimental results are as follows: the human skin patch test was performed for examples 6 and 9.
Table 5 examples 6 and 9 patches of human skin
The test result shows that 30 people have no adverse skin reaction.
And (3) testing the recovery condition of the abundance and diversity of microorganisms:
bacteria occupying the human body retain the 16S ribosomal RNA (rRNA) gene, a specific gene region of which can be used for systematic classification. This makes the 16S rRNA gene a molecular tag that can be used to identify individual members of a bacterial community. Skin microorganisms were collected using a non-invasive swab. After the microbial cells are lysed, microbial DNA is isolated. Then, selective PCR (polymerase chain reaction) amplification is carried out on the DNA by using a primer taking the 16S rRNA gene as a target, a PCR product is sequenced, and after a DNA sequence is obtained, basic sequence processing, species classification, diversity analysis and community comparison are carried out by using a biological information tool.
15 volunteers were selected, and four sites (A, B, C, D) were subjected to tape stripping on day 0, while the fifth site (E) was not subjected to stripping. Sites a, B, and C were then treated with the test products and volunteers were allowed to continue using the test products twice (morning and evening) on the selected sites for the next seven days. The microbial community was analyzed before the tape stripping operation, and on days 2 and 7 after tape stripping. Where A is the placebo group, B is example 6, C is example 9, D is the negative control group (untreated, compromised), and E is the positive control group (untreated, not compromised). The recovery of the abundance and diversity of the microorganism group was observed with the abundance and diversity of the E site set to 100%.
As in fig. 1-2, examples 6, 9 had a greater degree of recovery in terms of microbial enrichment on day two, and recovered to nearly the positive control group on day seven, whereas placebo recovered more slowly and less well; in terms of microbial diversity, examples 6 and 9 had a higher degree of recovery at day seven relative to the placebo group, closer to the positive control group. The above results demonstrate that the composition has a positive effect on restoring the abundance and diversity of skin microbiota, and is able to restore the homeostasis of skin microbiota.
Skin barrier repair test and texture test:
1) Skin barrier repair test:
30 female volunteers between 25 and 45 years old were selected, and each index was measured using an instrument 2 times a day for 28 days using an emulsion containing 1% by mass of the composition obtained in example 6, example 9 and comparative examples 1-2 and a blank emulsion, and the results are shown in fig. 3.
2) Skin texture testing:
figure 4 is a graph of skin texture before and after use of different compositions. As can be seen from fig. 4, the compositions obtained in examples 6 and 9 have a significant effect of improving skin texture.
It will be understood by those skilled in the art that the foregoing is only an exemplary embodiment of the present invention, and is not intended to limit the invention to the particular forms disclosed, since various modifications, substitutions and improvements within the spirit and scope of the invention are possible and within the scope of the appended claims.
Claims (10)
1. The composition with the effects of moisturizing, repairing and restoring skin stability is characterized by comprising red yeast rice fermentation liquor and lactobacillus fermentation lysate.
2. The composition with the effects of moisturizing, repairing and restoring skin homeostasis according to claim 1, wherein the mass ratio of the red yeast rice fermentation broth to the lactobacillus fermentation lysate is 1-15:1-10; preferably, the mass ratio of the red yeast rice fermentation liquid to the lactobacillus fermentation lysate is 1-10:1-3; more preferably, the mass ratio of the red yeast rice fermentation liquid to the lactobacillus fermentation lysate is 3-4:1.
3. the composition with the effects of moisturizing, repairing and restoring skin homeostasis according to claim 1, wherein the preparation method of the red yeast rice fermentation liquid comprises the following steps: culturing an monascus strain on a slant culture medium, washing spores with sterile water, filtering, oscillating to prepare uniform spore suspension, inoculating the spore suspension into a liquid culture medium, and performing shake culture to obtain a liquid seed solution; cleaning rice, draining, crushing, adding water, stirring, gelatinizing, cooling to room temperature, inoculating monascus liquid seed liquid, stirring and fermenting at 25-45 ℃, and performing spray drying after completion to obtain monascus fermentation products; adding deionized water into the obtained monascus fermentation product, inoculating lactobacillus, stirring and fermenting; after fermentation, sterilizing and centrifuging to obtain supernatant fluid, namely the red yeast fermentation broth.
4. The composition with moisturizing, repairing and skin-stabilizing effects according to claim 1, wherein the lactobacillus fermentation lysate is prepared by a method comprising the following steps: taking lactobacillus strains, carrying out streak constant-temperature culture in a slant culture medium, washing the strains, and centrifuging to obtain lactobacillus suspension; inoculating the lactobacillus suspension into a liquid culture medium, performing activated seed culture to obtain lactobacillus seed liquid, inoculating into a fermentation tank, stirring, ventilating, and culturing until fermentation is finished; filtering to obtain thallus, and breaking cell wall to obtain lactobacillus fermentation lysate.
5. The composition with the effects of moisturizing, repairing and restoring skin homeostasis according to claim 1, wherein the composition further comprises a stabilizer; preferably, the stabilizer is one or more of glycerol, ethylhexyl glycerol, 1, 2-hexanediol, hexanediol and pentanediol; more preferably, the ratio of the mass of the red yeast rice fermentation liquid and the lactobacillus fermentation lysate to the stabilizer is 0.3-5:1.
6. a method for preparing the composition with the effects of moisturizing, repairing and restoring skin homeostasis according to any one of claims 1 to 5, wherein the method comprises the following steps:
1) Preparing a seed solution: culturing an monascus strain on a slant culture medium, washing spores with sterile water, filtering, oscillating to prepare uniform spore suspension, inoculating the spore suspension into a liquid culture medium with the inoculation amount of 1-10%, and culturing for 1-3d by a shaking table to obtain a liquid seed solution;
2) Washing rice, draining, crushing, adding water and stirring, wherein the water-material ratio is controlled to be 8-12:1, gelatinizing, cooling to room temperature, and inoculating monascus liquid seed solution with the inoculation amount of 4-10%;
3) Fermenting at 25-45 deg.C for 2.5-3.5 days, stirring, fermenting, and spray drying to obtain Monascus fermentation product;
4) Adding 25-35 times of deionized water into the obtained monascus fermentation product, inoculating 8-12% of lactobacillus, stirring and fermenting;
5) After fermentation, sterilizing and centrifuging to obtain supernatant fluid, namely red yeast fermentation liquor;
6) Taking lactobacillus strains to perform streak culture in a slant culture medium, washing the strains after constant-temperature culture, and centrifuging to obtain lactobacillus suspension;
7) Inoculating the lactobacillus suspension into a liquid culture medium, and performing activated seed culture;
8) Inoculating the lactobacillus seed solution into a fermentation tank, stirring, ventilating, and culturing until the fermentation is finished;
9) Filtering to obtain thallus, and breaking cell wall to obtain lactobacillus fermentation lysate;
10 Mixing the obtained lactobacillus fermentation lysate into the obtained red yeast fermentation liquid, stirring and mixing uniformly, adding a stabilizer and an additive, mixing, and filtering to obtain the lactic acid bacteria fermented red yeast rice.
7. The method for preparing the composition for moisturizing, repairing and restoring skin homeostasis according to claim 6, wherein the spore suspension concentration in the step 1) is 1x10 5 -1×10 7 CFU/mL。
8. The method for preparing the composition with the effects of moisturizing, repairing and restoring skin homeostasis according to claim 6, wherein the stirring speed in the step 8) is 100 to 300r/min, and the ventilation rate is 0.3 to 0.6L/min; preferably, the stabilizer is one or more of glycerol, ethylhexyl glycerol, 1, 2-hexanediol, hexanediol and pentanediol; more preferably, the ratio of the mass of the red yeast rice fermentation liquid and the lactobacillus fermentation lysate to the stabilizer is 0.3-5:1.
9. the use of the composition with moisturizing, repairing and skin homeostasis effects according to any one of claims 1-5, wherein the use is adding the composition to a cosmetic.
10. The composition with the effects of moisturizing, repairing and restoring skin homeostasis according to claim 9, wherein the cosmetic is one or more of a aqua, an emulsion, a spray, a cream, a mask and a foundation.
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| RU2799936C1 (en) * | 2023-05-25 | 2023-07-14 | Федеральное государственное автономное образовательное учреждение высшего образования "Российский университет дружбы народов имени Патриса Лумумбы" (РУДН) | Method of manufacturing a composition for the care of facial prostheses |
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| RU2799936C1 (en) * | 2023-05-25 | 2023-07-14 | Федеральное государственное автономное образовательное учреждение высшего образования "Российский университет дружбы народов имени Патриса Лумумбы" (РУДН) | Method of manufacturing a composition for the care of facial prostheses |
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