KR102161025B1 - Shrimp feed composition comprising Leaves and Steams of Cudrania tricuspidata ferment liquids and powder - Google Patents

Abstract

The present invention relates to a shrimp feed composition comprising a fermented extract and powder using Cudrania tricuspidata leaves and stems. The shrimp feed composition of the present invention includes a mixture consisting of a Cudrania tricuspidata fermented extract produced by fermenting Cudrania tricuspidata leaves and stems, and Cudrania tricuspidata fruit-derived powder produced by drying a Cudrania tricuspidata fruit water extract. Provided is the shrimp feed composition capable of improving productivity and health of shrimps through good ingredients of Cudrania tricuspidata by allowing the good ingredients of the Cudrania tricuspidata to be easily transferred to the body of the shrimps, and capable of reducing the use of antibiotic at the same time.

Description

Shrimp feed composition comprising Leaves and Steams of Cudrania tricuspidata ferment liquids and powder}

The present invention relates to a composition for shrimp feed comprising a fermented extract and powder using Cudrania leaves and stems, and more specifically, to facilitate transfer of good ingredients of Cudrania tree into the body of shrimp, so that the good ingredients are the productivity of shrimp. And it relates to a composition for feed shrimp that can reduce the use of antibiotics and at the same time to improve health.

Shrimp farming production was showing a rapid increase every year until 2014, but since 2015, it has been decreasing due to various causes, such as weather changes such as heavy rains and typhoons, and economic problems caused by fluctuations in market prices. The greatest death was caused by the occurrence of.

In other words, aquaculture target organisms, including shrimp, have a higher rate of disease infection than other organisms due to the peculiarity of the water environment.

In particular, Korea has a higher incidence of diseases than other countries because it is aquaculture and rearing organisms with a high density compared to its water surface area.Therefore, antibiotics such as sulfa drugs, chemotherapy agents, and complex antibacterial agents are used excessively. As a result, it poses a threat to the food safety of the people. For this reason, studies on the development of feed additives using various natural materials that can replace these synthetic antibiotics have been conducted recently.

Among them, various functional substances are being developed for Cudrania tricuspidata , which is known as a material that does not suffer from pests and pests because of the immunity of the plant itself.This Cudrania tricuspidata is a deciduous small arbor, leaves in oriental medicine. The part is called embroidered foliage, and it is used to treat eczema, mumps, pulmonary tuberculosis, bruises, acute arthritis, etc., and the neck, root skin and fruit have anti-oxidation, anti-diabetes, cold prevention, anti-cancer, anti-aging, etc. It is known to have excellent efficacy in treating the disease, and it is one of the newly emerging materials so that all of the stems, leaves, skins, and fruits are used as medicines because it has a warm nature and is not poisonous.

In particular, through recent studies, Cudrania leaves have a higher content of nutrients and physiologically active substances such as protein, dietary fiber, vitamin B ₁ and vitamin B ₂ , calcium, iron, potassium, GABA and rutin compared to green tea leaves and general mulberry leaves. Appears to be.

Therefore, the aforementioned Cudrania tree is extracting a variety of effective ingredients through the analysis of its composition, and recently, as various health promotion and treatment effects have been known, its use has gradually expanded to reach new uses such as animal feed or aquaculture feed. It is being pioneered.

However, despite having various pharmacological actions, these Cudrania trees not only exhibit a unique aromatic characteristic, but also a bitter taste. Due to the characteristics of this aromatic and bitter taste, they can give a feeling of rejection to ingesting organisms, and to maximize the efficacy of Cudrania trees. Although research has been actively conducted, the development of functional materials that are enhanced than the original pharmacological effects of herbal medicines is required because Cudrania tree is not easy to consume as a herbal medicine, it is difficult to process raw materials, and its pharmacological activity per unit weight is not strong. have.

1. Republic of Korea Patent Publication No. 10-1852336'Feed additive composition for shrimp containing lactic acid bacteria complex cultured in industrial medium and its manufacturing method' 2. Republic of Korea Patent Publication No. 10-2020-0018997'Feed composition for white leg shrimp containing Spirulina as an active ingredient'

It is an object of the present invention to contain a large amount of functional ingredients of Cudrania tree itself by using a fermented extract of Cudrania tree leaves and branches obtained by introducing a specific fermentation technique and a powder derived from Cudrania tree fruit. It is to provide a shrimp feed composition that has a high absorption rate so that the good ingredients of Cudrania tree can help improve the productivity and health of shrimp.

It also aims to provide shrimp feed that can reduce the use of antibiotics.

The composition for shrimp feed of the present invention for achieving the above object comprises a mixture consisting of a Cudrania tree fermented extract prepared by fermenting Cudrania tree leaves and stems, and a Cudrania tree fruit-derived powder prepared by drying the Cudrania tree fruit water extract. It is characterized by including.

In addition, the fermentation extract is characterized in that it is prepared by immersing Cudrania leaves and stems in a mixture of Bacillus amyloliquefaciens culture, microcrystalline cellulose, and dextrin to ferment it, and then extracting it.

In addition, the mixture is characterized in that it is composed by mixing the Cudrania tree fermented extract and the Cudrania tree fruit-derived powder at a weight ratio of 8:1-2.

In addition, the mixture is characterized in that it is contained in 1 to 5% by weight based on the total weight of the feed composition.

Such, the shrimp feed composition is characterized in that it further comprises fish meal, wheat flour, molten soybean meal, squid liver powder, corn powder, fish oil, vitamins, minerals, choline chloride, lecithin, calcium monophosphate, and limestone.

Another method of manufacturing a shrimp feed according to the present invention is to prepare a Cudrania tree fermented extract by immersing Cudrania leaves and stems in a mixed solution consisting of Bacillus amyloliquefaciens culture medium, microcrystalline cellulose and dextrin, and then extracting them. The first step, the second step of preparing a Cudrania tree fruit-derived powder by drying and pulverizing the Cudrania tree fruit water extract obtained by extracting the Cudrania tree fruit with water, and mixing the Cudrania tree fermentation extract and the Cudrania tree fruit-derived powder to prepare a mixture In the third step of manufacturing, fish meal, wheat flour, molten soybean meal, squid liver powder, corn powder, fish oil, vitamins, minerals, choline chloride, lecithin, monocalcium phosphate, limestone are added to the mixture and mixed to prepare a composition for shrimp feed. It characterized in that it comprises a fourth step and, a fifth step of producing a shrimp feed by molding the composition for shrimp feed into a compressed pellet shape.

The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems that are not mentioned can be clearly understood by those of ordinary skill in the technical field to which the present invention belongs from the following description. There will be.

According to the present invention, as shrimp feed including the developed Cudrania tree leaves, fermented extract of eggplant and powder derived from Cudrania tree fruit is fed to farmed shrimp, there is an effect of reducing the use of antibiotics by increasing immunity. As the gain rate, survival rate, growth capacity, and feed utilization rate increase, it can be usefully used as an eco-friendly shrimp feed.

In particular, by grafting functional ingredients of Cudrania tree, a specialty crop of Sinan-gun, the strategy of using Cudrania trees differentiated by the development of Cudrania shrimp feed can achieve a stable income increase effect for farmers who cultivate Cudrania trees and fishermen who grow shrimp.

1 is a view showing a manufacturing process diagram of a shrimp feed according to Example 1 of the present invention.
FIG. 2 is a diagram showing the nucleotide sequence of Bacillus amyloliquefaciens strain used to prepare a Cudrania tree fermented extract according to Example 1 of the present invention.
3 is a view showing the growth of shrimp by feeding shrimp feeds according to Example 1 and Comparative Examples 1 and 2 to shrimp.

Hereinafter, preferred embodiments and experimental examples of the present invention will be described in detail with reference to the accompanying drawings, and detailed descriptions of known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted.

The present invention relates to a composition for a shrimp feed comprising a fermented extract and powder using Cudrania leaves and stems, and will be described in detail based on the following examples.

The active ingredient of the composition for feed for shrimp of the present invention is a mixture of Cudrania tree fermentation extract obtained by introducing specific fermentation technology to Cudrania tree leaves and stems, and Cudrania tree fruit derived from Cudrania tree fruit obtained by water extraction and drying the same. It is a mixture.

To explain, Cudrania tree is a material known to contain functional ingredients that exhibit various effects such as antibacterial and antioxidant effects. Among them, ingredients such as lutein, gaba, morin, and mortin are known as antioxidants. Roxydecanoic acid (4-hydroxydecanoic acid), ferulic acid (Ferulic acid), vanillin (vanillin), gallic acid (Gallic acid), rutin hydrate (Rutin hydrate), P-coumaric acid (P-coumaric acid), caffeic acid ( Caffeic acid), gentisic acid, syringic acid, quercetin, vanillic acid, chlorogenic acid, protocatechuic acid, camphorol ( Kaempferol), Naringenin, Salicylic acid, Hesperidin, Biochanin A, Oxyresveratrol, Chrysin, etc. have been reported to contain a large amount. .

These functional ingredients are contained in many leaves and branches as well as the fruit of the Cudrania tree. However, in general, the fruit of the Cudrania tree can be easily used as a functional material, but the remaining by-products such as leaves and branches have a problem that their utility is poor.

In other words, despite containing a large amount of functional ingredients of Cudrania leaves and branches, the intake rate in the body decreases by itself, and there is a problem that indicates indigestion of the feed target even after feeding. Yes, it is almost being discarded.

Accordingly, the present inventors confirmed the business possibility through a specification test to see if the Cudrania tree was used as a functional food material, as well as whether it affects the productivity of farmed shrimp, and in particular, by using the leaves and stems of the Cudrania tree which have been discarded It came up with a way to activate it as an additional source of income for farms.

In other words, a specific fermentation technique was introduced for Cudrania tree leaves and stems to prepare a Cudrania tree fermented extract, and water extracts were prepared for Cudrania tree fruit, and then dried to prepare powder derived from Cudrania tree fruit. When feeding shrimp feed containing a mixture prepared by mixing the two substances in a specific ratio to farmed shrimp, it was confirmed that the increase rate, survival rate, growth capacity, and feed utilization rate of shrimp were increased.The mixture composed as described above was used for shrimp feed. It can be seen that it is suitable as an active ingredient of the composition.

At this time, preferably, the mixture is characterized in that it is contained in an amount of 1 to 5% by weight based on the total weight of the feed composition, and if it is contained in less than 1% by weight, the effect of increasing the shrimp production for the purpose of the present invention without the use of additional antibiotics It is difficult to obtain, and if it is contained in excess of 5% by weight, it is difficult to obtain shrimp gain rate due to the fact that it is not easy to eat shrimp due to the peculiar scent of Cudrania or other mixed feed ingredients.

The composition for shrimp feed configured as described above is characterized in that the shrimp feed is molded into a compressed pellet shape so that the shrimp feed the feed easily and at the same time so that it is not easily released from water, and in more detail, shown in FIG. 1 As described above, fermentation extract production step (S10) of Cudrania tree, Cudrania tree fruit-derived powder production step (S20), Cudrania tree mixture production step (S30) by mixing the fermentation extract and the powder, fish meal in the Cudrania tree mixture, Wheat flour, peeled soybean meal, squid liver powder, corn powder, fish oil, vitamins, minerals, choline chloride, lecithin, calcium monophosphate, and limestone are added and mixed to prepare a composition for shrimp feed (S40), and the shrimp feed composition is molded to Shrimp feed production comprising a shrimp feed composition capable of enhancing immunity and increasing the gain rate, survival rate, growth capability, and feed utilization rate of shrimp by containing a large amount of functional ingredients of Cudrania tree itself, including the feed manufacturing step (S50) It is a major feature that is possible, and this will be described in detail as follows.

<Production step of shrimp feed containing fermented extract and powder using Cudrania leaves and stems of the present invention>

1. Step 1: Manufacturing step of fermented extract of Cudrania tree (S10)

In this step, the leaves and branches of Cudrania tree are immersed in a mixture of Bacillus amyloliquefaciens culture, microcrystalline cellulose, and dextrin, fermented, and extracted to prepare a fermented extract.

To explain, Cudrania leaves and branches are known to contain various functional ingredients such as immunity enhancing effect, but their scent is strong, and when powdered as it is, it causes difficulties in digestion, making it difficult to use easily as a feed material. Therefore, Bacillus amyloliquefacience culture medium is used to make it easier for shrimp to consume and to extract a large amount of the functional ingredients of the Cudrania tree leaves and branches, but so that the bacteria contained in the culture medium grow well. Microcrystalline cellulose and dextrin are added together and mixed and fermented to maximize the functional ingredients of the Cudrania leaves and branches.

In other words, the mixed solution used for the fermentation of Cudrania leaves and branches is characterized by consisting of Bacillus amyloliquefaciens culture solution, microcrystalline cellulose, and dextrin, and more preferably, the Bacillus amyl based on the total weight of the mixed solution. It is characterized in that the Lorlique Fascience culture solution is 70 to 85% by weight, the microcrystalline cellulose is 10 to 20% by weight, and the dextrin is 1 to 10% by weight.

At this time, the Bacillus amyloliquefacience culture medium used is licensed for edible use, and this Bacillus amyloliquefaciens culture solution can increase the immunity enhancing effect by increasing the lutein or gaba component of Cudrania leaves and branches compared to other strains. By doing so, such a Bacillus amyloliquefaciens strain can be easily distributed by anyone from the Korea Research Institute of Bioscience and Biotechnology, the Korea Microbial Conservation Center, and more preferably Bacillus amyloliquefaciens having the nucleotide sequence shown in FIG. It is best to apply the strain. This is a strain isolated by the inventor of the present invention, derived through 16S RNA analysis, has a length of 1240 bp, and is a strain showing 100% homology with Bacillus amyloliquefacie ns UMAF6614, so no separate deposit was made.

The culture medium may mean a material including all substances contained in the culture medium in which the strain is cultivated, for example, it may mean a substance including metabolites or secretions that are the result of culturing the strain, or a lysate thereof, and the strain itself is also cultured. May be contained in water. In addition, the culture medium may be cultured according to a method commonly used in the art.

As the medium, a conventional medium may be used, and after inoculating the Bacillus amyloliquefaciens strain in a liquid medium, culture it at 35 to 37°C for 24 to 72 hours to a concentration of 1×10 ⁷ CFU/ml or more It is characterized by preparing a microbial culture solution of, and more preferably, after inoculating the microbial strain in the liquid medium, the aeration amount of 0.05 to 0.4 vvm (vol/vol/min) for 24 to 72 hours at 35 to 37°C and It is most suitable to culture at a stirring speed of 100 to 200 rpm (rotary/min) and an internal pressure of 0.1 to 1 kgf/㎠.

The Bacillus amyloliquefacian culture solution thus prepared is characterized by applying 70 to 85% by weight based on the total 100% by weight of the mixed solution.If it is less than 70% by weight, the content is too small to obtain the desired efficacy of the present invention. If it exceeds 85% by weight, the blending ratio with other materials does not match, so there is a concern that the desired efficacy of the present invention may not be obtained.

The microcrystalline cellulose is used as a medium source for the bacteria, so that the proliferation of the bacteria can be maximized. Accordingly, the microcrystalline cellulose is characterized by applying 10 to 20% by weight based on the total 100% by weight of the mixed solution.If it is less than 10% by weight, the content is too small to obtain the effect of increasing the growth of bacteria, and 20% by weight When it exceeds %, there is a fear that the desired efficacy of the present invention may not be obtained because the mixing ratio with other materials is not appropriate.

The dextrin is a generic term for starch decomposition products, roasted dextrin decomposition products, or derivatives thereof obtained by decomposing starch or roasted dextrin with a starch decomposing enzyme such as acid or amylase. Dextrin includes roasted dextrin, digestible dextrin, indigestible dextrin and their hydrides, and such dextrin, like the microcrystalline cellulose, allows the proliferation of the bacteria to be increased to the maximum.

Accordingly, the dextrin is preferably in the range of 1 to 10% by weight based on the total 100% by weight of the mixed solution, and when it is contained in less than 1% by weight, it is difficult to obtain a growth effect of bacteria compared to the addition, and when it exceeds 10% by weight Due to the unique taste of dextrin itself, the demand for shrimp feed decreases.

After immersing 250 to 300 g of the Cudrania leaves and branches per 1 L of the mixture thus prepared, fermentation is carried out at 20 to 30°C for 30 to 60 days, and then extracted to prepare a Cudrania fermented extract. .

At this time, the fermentation condition is a condition applied for the purpose of maximizing lutein or gaba components among the functional ingredients of the Cudrania leaves and branches, and when applied for less than 30 days or less than 24°C, derivation of the desired functional ingredients This is not enough, and if it exceeds 60 days or exceeds 30℃, there is a concern that component destruction due to the excess may occur.

In addition, as the extraction method, it is preferable to apply a brewing or pressed extraction method in order to minimize the destruction of functional components.

2. Step 2: Manufacturing step of powder derived from Cudrania tree fruit (S20)

In this step, the water extract obtained by extracting the fruit of the Cudrania tree with water is dried and then pulverized to prepare a powder form.

Explaining, it is reported that the fruit of the Cudrania tree contains a large amount of functional ingredients. Therefore, it is most suitable to apply the water extraction method in order to extract the maximum functional ingredients from the fruit of the Cudrania tree and to be used as a feed material for shrimp. In other words, it is because the functional ingredients contained in the Cudrania tree fruit can be sufficiently obtained even by applying the water extraction method.

At this time, in order to obtain ease of extraction, it is characterized in that the amount of water per 30 g of Cudrania fruit is used in the range of 250 to 1000 ㎖, and if the content of water per 30 g of Cudrania fruit is less than 250 mL, the extracted amount is too small and components included in the material Is less extracted, and if the content of water per 30 g of Cudrania tree fruit exceeds 1000 ml, the concentration of the stock solution becomes too light.

As an extraction method, in order to minimize the destruction of functional ingredients in the fruit of the Cudrania tree, either brewing or pressed extraction is selected and applied.

When the water extract of Cudrania tree fruit prepared in this way is applied as it is, the moisture content is too large, and there is a concern that the process may not be easy when it is molded into shrimp feed.

Accordingly, in order to meet the appropriate moisture content, the water extract of Cudrania tree fruit is dried and then prepared in a powder form. At this time, it is more preferable to apply freeze-drying in order to powder a sieve maintaining the functional ingredients as much as possible. Best.

In other words, the freeze-drying method is a method of obtaining a dried product by removing moisture by sublimating ice by freezing and depressurizing an aqueous solution or material containing a large amount of moisture. This is not a method of directly heating a sample, but the destruction of nutrients. It is effective in suppression. After drying in this way, in order to be well blended with other materials added in addition to the following mixture, it is preferable to prepare a powder derived from Cudrania tree fruit by grinding to a particle size of about 300 to 500 μm.

3. The third step: Cudrania tree mixture manufacturing step (S30)

In this step, the fermented extract of the Cudrania tree of the first step and the powder derived from the Cudrania tree fruit are mixed to prepare a Cudrania tree mixture.

In other words, the Cudrania tree leaf and eggplant fermentation extract obtained through the method of maximizing the functional ingredients of each Cudrania tree part and the Cudrania tree fruit-derived powder are mixed and used to apply as an active ingredient of the shrimp feed composition. The mixing ratio of the above ingredients should be applied according to the growth power of the shrimp.

In other words, if the mixing ratio of the above ingredients is not appropriate, the content of the extracted functional ingredient is small, making it difficult to lower the mortality rate compared to the existing shrimp feed, or rather, the taste and aroma of the shrimp intake decreases.

Thus, it is preferable to prepare a mixture by mixing the Cudrania tree fermentation extract of the first step and the Cudrania tree fruit-derived powder of the second step in a ratio of 8:1 to 2 by weight, and the powder derived from the Cudrania fruit outside the weight range When the content of the Cudrania tree fermented extract is the same as or relatively large, the functional ingredient content of the Cudrania tree is small, making it difficult to lower the mortality rate of shrimp.

4. Fourth step: Shrimp feed composition manufacturing step (S40)

In this step, fish meal, wheat flour, peeled soybean meal, and squid are added to the Cudrania tree mixture of the third step, as additional ingredients commonly used in shrimp feed, in order to enhance the taste and mix the nutrients essential for shrimp breeding. It is characterized by manufacturing a composition for shrimp feed, further comprising liver powder, corn powder, fish oil, vitamins, minerals, choline chloride, lecithin, monocalcium phosphate, and limestone.

At this time, the additional ingredients are materials used in conventional shrimp feed, but a suitable mixing ratio with the Cudrania tree mixture is very important to improve the productivity and health of the shrimp. Thus, the composition for the shrimp feed is Cudrania tree mixture 1 to 5% by weight, the fish meal 30 to 55% by weight, the wheat flour 15 to 20% by weight, the molten soybean meal 10 to 22% by weight, the squid liver powder 10 to 15% by weight , The corn powder 5 to 10% by weight, the fish oil 1 to 5% by weight, the vitamin 0.1 to 1% by weight, the mineral 0.1 to 1% by weight, the choline chloride 0.1 to 1% by weight, the lecithin 0.1 to 1% by weight , It is best to be composed of 0.1 to 1% by weight of the calcium monophosphate and 1 to 5% by weight of the limestone.

In other words, when the Cudrania tree mixture is contained in an amount of less than 1% by weight, it is difficult to provide a shrimp feed containing a large amount of rutin or gaba components for the purpose of the present invention, and when it exceeds 5% by weight, the feed intake rate of the shrimp decreases. do.

The fish meal is processed for feed through processing methods such as self-cooking, compression, drying, and crushing of tofu, bones, skins, tails, and intestines, which are by-products of fish processing, such as multi-catch fish or caught fish unsuitable for processing. It is used to supply proteins, essential amino acids, essential fatty acids, and energy necessary for shrimp growth, and more preferably, sardine fish meal and tuna fish meal are mixed and used, but each mixing ratio is 1 to 2: 1 weight ratio. good.

If the fishmeal content is less than 30% by weight, the supply of protein and essential amino acids decreases, making it difficult to obtain the shrimp gain. If it exceeds 55% by weight, it is not only possible to obtain an increased shrimp gain due to excess nutrition. There is a concern that the cost of shrimp feed will increase due to the increase in fishmeal consumption.

The wheat flour is a carbohydrate source that can increase the binding power of the feed composition and supply energy for the growth of shrimp, and when it is contained in less than 15% by weight, the binding power between the ingredients decreases, and there is a concern that the water stability of the feed decreases. If it exceeds 20% by weight, an imbalance with other nutrients occurs due to excessive supply of wheat flour, making it difficult to obtain a shrimp gain rate.

The soybean meal is a by-product remaining after crushing soybeans and extracting oil with hexane, which is a vegetable protein source with an average protein content of 44%.If it contains less than 10% by weight, nutritional deficiencies due to lack of protein source This may occur, and if it exceeds 22% by weight, there is a concern that shrimp growth may be slowed due to a decrease in palatability.

The squid liver meal is for supplying protein and fatty acids and phospholipids as well as the following fish oil. When it is contained in less than 10% by weight, nutritional deficiencies occur, making it difficult to obtain a high shrimp gain rate and 15% by weight. If it exceeds %, there is a concern that shrimp growth will be slowed due to a decrease in palatability.

The fish oil is for supplying essential fatty acids and energy, and contains a large amount of polyunsaturated fatty acids, and generally refers to oil obtained by boiling and pressing fat-rich meat such as sardines, herring, and mackerel. If such fish oil is contained in less than 1% by weight, the fluidity of the cells may not be improved and the vitality of the digestive tract may be lowered. If it exceeds 5% by weight, it is difficult to expect a synergistic effect according to the additional content.

The choline chloride, minerals, and vitamins are supplementary nutrients added for the growth characteristics and immunity of shrimp, and are mixed within the range of 0.1 to 1% by weight, respectively.If they are out of the weight range, they cannot serve as supplementary nutrients. Or, it is difficult to expect a synergistic effect due to the additional content, which causes the cost to rise.

The lecithin refers to a lipid mixture in which 50% or more of total lipids are phospholipids, and the most common types of phospholipids present in lecithin are phosphatidylcholine, phosphatidylethanolamine and phosphatidylinosyrol. The lecithin applied to the present invention may be a lecithin of vegetable origin such as soya lecithin or lecithin obtained from oily plants such as sunflower or colza, and the type is not particularly limited. In addition, the lecithin is characterized in that it is mixed within the range of 0.1 to 1% by weight, and when it is contained in less than 0.1% by weight, it becomes difficult to add the efficacy of lecithin, and when it exceeds 1% by weight, the synergistic effect according to the additional content is achieved. As it becomes difficult to expect, it causes cost increases.

The monocalcium phosphate is used for the purpose of supplying calcium and phosphorus to shrimp at the same time. If it is contained in less than 0.1% by weight, it becomes difficult to add the efficacy of monocalcium phosphate, and if it exceeds 1% by weight, the additional content As it becomes difficult to expect the resulting synergistic effect, it becomes the cause of cost increase.

The limestone is a kind of aqueous rock containing calcium carbonate as a main component, and serves to supply Ca. In this case, if it is contained in less than 1% by weight, there is a risk of nutritional deficiency, and if it exceeds 5% by weight, excessive nutritional use Rather, there is a problem that the shrimp gain rate is lowered due to the occurrence of the shrimp or the molding is not easily made during molding with the following shrimp feed.

In this step, depending on the growth status of the shrimp, that is, for fry, for breeding, or for sexual harassment, lysine cell protein and egg powder are optionally added to manufacture.

4. Step 5: Shrimp Feed Manufacturing Step (S50)

In this step, the shrimp feed composition is molded into a compressed pellet shape to produce a shrimp feed, which is the final invention.

More preferably, the prepared shrimp feed composition is processed at 100 to 120° C. for 0.5 to 1 minute through a compressor system to produce a compressed pellet shape, and the processing time is less than 100° C. or 0.5 In the case of only powdering, it is difficult to maintain the shape of a pellet, making it difficult to feed the shrimp feed. In the case of processing exceeding 120℃ or exceeding 1 minute, the productivity of the shrimp for the purpose of the present invention due to the destruction of nutrients. Becomes difficult to obtain.

The shrimp feed prepared in this way provides the optimal feed for shrimp farming, thereby increasing the increase rate, survival rate, growth capacity, and feed utilization rate of shrimp, and at the same time reducing the use of antibiotics, making it useful as an eco-friendly shrimp feed. Can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples, but these Examples and Experimental Examples are for illustrative purposes only and are not intended to limit the protection scope of the present invention.

<Example 1> Preparation of shrimp feed 1 containing Cudrania tree fermented extract and Cudrania tree fruit-derived powder

The fruits, leaves, and branches of Cudrania tree were purchased on the market, washed, dried, and prepared.

70ℓ (LA medium, 1×10 ⁷ CFU/ml) of Bacillus amyloliquefacience strain culture solution having the nucleotide sequence of FIG. 2 was prepared.

70ℓ of the Bacillus amyloliquefacience culture solution, 20 kg of microcrystalline cellulose, and 10 kg of dextrin were added and mixed to prepare a mixture.

Then, 300 g of Cudrania leaves and branches were put in 1 L of the mixed solution and immersed, and then fermented at 25° C. for 30 days, and then filtered and extracted to prepare a Cudrania tree fermented extract.

After adding 1 ℓ of purified water to 30 g of the prepared Cudrania tree fruit, 1 ℓ of purified water was added to pressurized extraction to prepare a Cudrania tree fruit water extract, which was freeze-dried at -40°C for 48 hours, and then pulverized to prepare a Cudrania tree fruit-derived powder.

A Cudrania tree mixture was prepared by mixing 80 ml of the thus prepared Cudrania tree fermentation extract and 20 g of the Cudrania tree fruit-derived powder.

Then, 1 g of the Cudrania tree mixture, 15 g of sardine meal, 15 g of tuna fish meal, 17 g of wheat flour, 21.2 g of molten soybean meal, 13 g of squid liver powder, 9.7 g of corn powder, 3 g of fish oil, 0.2 g of vitamin premix, 0.2 g of minerals, 0.2 g of choline chloride , 0.5 g of lecithin, 0.5 g of monocalcium phosphate, and 3.5 g of limestone were added and mixed to prepare a composition for shrimp feed and compressed at 100°C for 1 minute to prepare a shrimp feed 1 consisting of a compressed pellet shape of Ø1.6X2.0mm. .

<Comparative Example 1> Preparation of shrimp feed 2

Prepared in the same manner as in Example 1, except for the Cudrania tree mixture, and 1 g of the wheat flour was further added to prepare shrimp feed 2.

<Comparative Example 2> Preparation of shrimp feed 3

Prepared in the same manner as in Example 1, but by applying a mixture prepared by mixing 50 ml of the Cudrania tree ferment extract and 50 g of the Cudrania tree fruit-derived powder was applied to prepare shrimp feed 3.

<Comparative Example 3> Preparation of shrimp feed 4

It was prepared in the same manner as in Example 1, but when preparing the Cudrania tree mixture, shrimp feed 4 was prepared by applying Cudrania tree leaf and branch powder instead of the fermented extract.

<Experimental Example 1> Functional ingredient test by strain and part of Cudrania tree

1. Experiment method

Rutin and Gaba components among several functional components were measured by applying the extraction method for each strain (Bacillus amyloliquefaciens, Bacillus subtilis, and Lactobacillus genus) and Cudrania tree parts.

At this time, the extraction conditions were reflux cooling, and 100 ml of each extraction solvent was added to 5 g of the sample. The reflux cooling was extracted at 80°C for 3 hours, then filtered under reduced pressure with a filter paper (Whatman No2) and concentrated, and the concentrate was It was quantified into a 50 ml flask, filtered through a 0.45 μm syringe filter, and analyzed by HPLC.

2. Experiment result

Rutin μg/kg GABA mg/kg Cudrania fruit
-Water extraction 33.13 70.7 Cudrania leaves & branches
-Water extraction 17.57 42.8 Cudrania tree fermented extract (Example 1)-Amylolique Fescience 90.23 888.59 Cudrania tree fermented extract
-Bacillus subtilis 75.11 365.77 Cudrania tree fermented extract
-Lactobacillus genus 65.78 467.9 Cudrania tree fruit fermented extract-Amyloliquefacience 10.2 45.6

As shown in Table 1, when fermenting Cudrania leaves and branches, it was confirmed that the contents of rutin and gava were applied differently for each applied strain.

In other words, the Bacillus amyloliquefaciens strain applied to Example 1 has significantly higher rutin and gaba content through fermentation than water extracts of existing Cudrania leaves and branches, whereas other strains Bacillus subtilis or When Lactobacillus subtilis was applied, it was confirmed that the degree of increase in the Gaba content was small.

In addition, it was confirmed that in the case of the Cudrania tree fruit, the water extraction process is suitable for increasing the content of rutin and gaba.

<Experimental Example 2> Confirmation of shrimp growth power

1. Experiment method

Shrimp growth power (gain rate, survival rate, feed utilization rate) was confirmed for the white leg shrimp fed the shrimp feeds of Example 1 and Comparative Examples 1,2.

2. Experiment result

As a result of the above experiment, as shown in FIG. 3, in the case of Example 1 containing a large amount of extract obtained by fermenting Cudrania tree leaves and branches, and Comparative Example 1 without adding it, and the same amount of the fermentation extract and the powder derived from Cudrania tree fruit water extract In the case of Comparative Example 2, it was confirmed that the weight gain according to feed feeding was similar,

On the other hand, in the case of Comparative Example 2, the mortality rate was similar to that of Comparative Example 1 in which Cudrania leaves and eggplant fermentation extracts were not added.In the extract obtained by fermenting Cudrania tree leaves and branches, a large amount of functional ingredients representing natural antibiotics were contained. It was found that there was almost no component in the powder derived from the water extract of Cudrania tree fruit. Accordingly, when the shrimp feed of Example 1 of the present invention is fed to farmed shrimp, it can be seen that it can be usefully used as an eco-friendly shrimp feed as it has an effect of reducing the use of antibiotics through immunity enhancement.

<Experimental Example 3> Confirmation of functional ingredients of Cudrania tree transferred into shrimp body

1. Experiment method

The functional ingredients of Cudrania tree which were transferred into the shrimp body were confirmed by targeting white-legged shrimp fed the shrimp feeds of Example 1 and Comparative Example 3.

2. Experiment result

The experimental results were shown in Table 2 below.

Analysis item Shrimp fed the feed of Comparative Example 3 Shrimp fed the feed of Example 1 Total flavonoid content (mg/kg) 316 493 Rutin(㎍/kg) 10.64 21.57 Salicylic acid(㎍/kg) - 28.50 Resveratrol(㎍/kg) 3.75 9.87

As shown in Table 2, the functional ingredients of Cudrania tree leaves and branches are extracted as much as possible according to the introduction of a specific fermentation technology as in Example 1, which was found to be well transferred into the shrimp body even when fed to farmed shrimp.

On the other hand, in the case of Comparative Example 3, in which Cudrania leaves and branches were powdered and fed as they are, it was found that the functional components of Cudrania tree leaves and branches were substantially absorbed into the shrimp body.

As described above, according to the present invention, there is an effect of reducing the use of antibiotics by enhancing immunity, and it can be seen that it can be usefully used as an eco-friendly shrimp feed as the increase rate, survival rate, growth capacity, and feed utilization rate of shrimp are increased. .

The present invention has been looked at by focusing on preferred embodiments and experimental examples, and those of ordinary skill in the art to which the present invention pertains are within the scope of the essential technical scope of the present invention. You can implement examples. Here, the essential technical scope of the present invention is shown in the claims, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (7)

Including a mixture consisting of a Cudrania tree fermented extract prepared by fermenting Cudrania tree leaves and stems, and a Cudrania tree fruit-derived powder prepared by drying the Cudrania tree fruit water extract,
The fermentation extract includes those prepared by immersing Cudrania leaves and stems in a mixed solution consisting of Bacillus amyloliquefacience culture solution, microcrystalline cellulose and dextrin to ferment, and then extracting them,
The mixture consisting of the Cudrania tree fermentation extract and the Cudrania tree fruit-derived powder includes that the Cudrania tree fermentation extract and the Cudrania tree fruit-derived powder are mixed in a weight ratio of 8:1 to 2,
The Bacillus amyloliquefaciens culture solution, the mixture consisting of microcrystalline cellulose and dextrin, based on the total weight of the mixture, the Bacillus amyloliquefacience culture solution is 70 to 85% by weight, the microcrystalline cellulose is 10 to 20% by weight , The dextrin comprises mixing in 1 to 10% by weight,
The composition for shrimp feed consisting of the mixture and the additional composition comprises 1 to 5% by weight of the mixture, 30 to 55% by weight of fish meal, 15 to 20% by weight of wheat flour, 10 to 22% by weight of peeled soybean meal, 10 to 15% by weight of squid liver powder, Corn powder 5-10 wt%, fish oil 1-5 wt%, vitamin 0.1-1 wt%, mineral 0.1-1 wt%, choline chloride 0.1-1 wt%, lecithin 0.1-1 wt%, monocalcium phosphate 0.1-1 % By weight, including 1 to 5% by weight of limestone,
Composition for shrimp feed.
delete delete delete delete A first step of immersing Cudrania tree leaves and stems in a mixture of Bacillus amyloliquefaciens culture medium, microcrystalline cellulose and dextrin to ferment them, and then extracting them to prepare a Cudrania tree fermentation extract;
A second step of manufacturing a Cudrania tree fruit-derived powder by drying and pulverizing the Cudrania tree fruit water extract obtained by extracting the Cudrania tree fruit with water;
A third step of preparing a mixture by mixing the Cudrania tree fermentation extract and the Cudrania tree fruit-derived powder;
A fourth step of preparing a composition for shrimp feed by mixing fish meal, wheat flour, molten soybean meal, squid liver powder, corn powder, fish oil, vitamins, minerals, choline chloride, lecithin, monocalcium phosphate, and limestone to the mixture, and,
Including; a fifth step of producing a shrimp feed by molding the composition for shrimp feed into a compressed pellet shape,
The Bacillus amyloliquefaciens culture solution, the mixture consisting of microcrystalline cellulose and dextrin, based on the total weight of the mixture, the Bacillus amyloliquefacience culture solution is 70 to 85% by weight, the microcrystalline cellulose is 10 to 20% by weight , The dextrin comprises mixing in 1 to 10% by weight,
The composition for shrimp feed consisting of the mixture and the additional composition is 1 to 5% by weight of the mixture, 30 to 55% by weight of the fish meal, 15 to 20% by weight of the wheat flour, 10 to 22% by weight of the peeled soybean meal, 10 to the squid liver powder 15% by weight, the corn powder 5 to 10% by weight, the fish oil 1 to 5% by weight, the vitamin 0.1 to 1% by weight, the mineral 0.1 to 1% by weight, the choline chloride 0.1 to 1% by weight, the lecithin 0.1 to 1% by weight, containing 0.1 to 1% by weight of the monocalcium phosphate, 1 to 5% by weight of the limestone,
Method for producing shrimp feed.
It is characterized by being manufactured by the manufacturing method of claim 6,
Shrimp feed.

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