KR102367647B1 - Production method of egg having anti-oxidant ability and blood circulation ability using the feed composition for chicken including rhus verniciflua extract, and egg produced by the same method - Google Patents

Abstract

The present invention relates to a method for manufacturing an egg having an antioxidant effect and a blood circulation improving effect using a chicken feed composition containing a sumac extract as an active ingredient, and an egg manufactured thereby and having an antioxidant effect and a blood circulation improving effect.

Description

A method for producing an egg having antioxidant and blood circulation improving ability using a chicken feed composition containing poison ivy extract as an active ingredient, and eggs produced thereby CHICKEN INCLUDING RHUS VERNICIFLUA EXTRACT, AND EGG PRODUCED BY THE SAME METHOD}

The present invention relates to a method for producing eggs having antioxidant effects and blood circulation improving effects using a chicken feed composition containing sumac extract as an active ingredient.

In addition, the present invention relates to an egg produced by the above method having an antioxidant effect and blood circulation improving effect.

Eggs are nutritious and well-balanced food. Eggs contain high-quality protein in high concentrations, but also contain various nutrients such as unsaturated fatty acids, iron, and vitamins. Currently, egg production is being made in a way that enhances the beneficial ingredients for the human body among the ingredients inherent in natural eggs and lowers the content of ingredients harmful to the human body.

In recent years, as interest in health has increased, functional eggs with various health-improving functions have been developed not only to increase the content of beneficial ingredients in eggs and to increase egg productivity, but also to feed chickens with a specific feed. Attempts to produce are continuing. For example, in Korean Patent No. 10-2172369, a mixture of barley sprout powder and arrowroot juice is enzymatically treated with alpha-amylase and cellulase, and the enzyme-treated product is fermented with Saccharomyces creviciae. Disclosed is a method for producing functional eggs with increased saponarin content by ingesting barley sprout powder feed to laying hens.

On the other hand, lacquer tree has various effects such as insecticidal effect, sterilization effect, immune boosting effect, fatigue recovery, atopy improvement, hangover relief, etc., and contains a large amount of flavonoids. Development of a functional egg obtained by preparing a chicken feed composition using an extract of poison ivy as a main component and feeding it to chickens has not been reported so far.

Accordingly, the present applicant completed the present invention by feeding chickens with a chicken feed composition containing sumac extract as a main component to produce eggs having various functions such as antioxidant activity and blood circulation improving ability.

Republic of Korea Patent Registration No. 10-2172369

A first object of the present invention is to provide a method for producing eggs having antioxidant effects and blood circulation improving effects from fertilized chickens by feeding a chicken feed composition containing sumac extract as a main component.

A second object of the present invention is to provide an egg that is produced by the above method and has an excellent antioxidant effect and blood circulation improving effect.

The object of the present invention is not limited to the technical problem as described above, and another technical problem may be derived from the following description.

In order to achieve the first object, the present invention provides a first step of preparing a mixed extract comprising sumac extract, pork potato extract, red bean extract and green tea extract; a second step of primary fermenting the mixed extract to obtain a primary fermented product; a third step of secondary fermentation of the primary fermented product to obtain a secondary fermented product; a fourth step of preparing a chicken feed composition comprising the secondary fermented product, soybean meal, wheat, corn and rice bran; a fifth step of feeding the chicken feed composition to chickens; and a sixth step of obtaining eggs from chickens fed with the chicken feed composition; provides a method for producing eggs having antioxidant activity and blood circulation improving ability, including.

The mixed extract may include the sumac extract, pork potato extract, red bean extract and green tea extract in a weight ratio of 10 to 15: 0.1 to 1: 0.1 to 1:1.

The primary fermentation may be carried out by inoculating the mixed extract with a lactic acid bacteria seed containing Lactobacillus casei at 0.5 to 1% (v/v).

The primary fermentation may be carried out for 40 to 50 hours at pH: 5 to 6 , temperature: 30 to 40 ℃ conditions.

The secondary fermentation may be carried out by inoculating the primary fermented product with a lactic acid bacteria seed containing Lactobacillus acidophilus at 1 to 2% (v/v).

The secondary fermentation may be carried out for 10 to 20 hours under conditions of pH: 5 to 6 , and temperature: 35 to 40 °C.

The chicken feed composition may include 5 to 20% by weight of secondary fermented product, 10 to 30% by weight of soybean meal, 10 to 30% by weight of wheat, 7 to 15% by weight of corn, and 3 to 10% by weight of rice bran based on the total weight there is.

In the fifth step, the chicken feed composition may be fed to chickens for at least 2 months or more.

In order to achieve the second object, the present invention provides a first step of preparing a mixed extract comprising sumac extract, pork potato extract, red bean extract and green tea extract; a second step of primary fermenting the mixed extract to obtain a primary fermented product; a third step of secondary fermentation of the primary fermented product to obtain a secondary fermented product; a fourth step of preparing a chicken feed composition comprising the secondary fermented product, soybean meal, wheat, corn and rice bran; a fifth step of feeding the chicken feed composition to chickens; and a sixth step of obtaining eggs from chickens fed with the chicken feed composition.

In the present invention, by feeding chickens with a chicken feed composition containing sumac extract as an active ingredient, it is possible to produce functional eggs that contain a large amount of useful ingredients of sumac tree and have antioxidant effects and blood circulation improving effects.

1 is a diagram illustrating a flow of a method for producing eggs according to an embodiment of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art can easily implement them. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein. And in order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted.

The terms or words used in the specification and claims of the present invention are not to be construed as limited in their ordinary or dictionary meanings, and the inventor can appropriately define the concept of the term in order to best describe his invention. Based on the principle, it should be interpreted as meaning and concept consistent with the technical idea of the present invention.

In the entire specification of the present invention, when a part "includes" a component, it means that other components may be further included, rather than excluding other components, unless otherwise stated. .

Throughout the specification of the present invention, "A and/or B" means A or B, or A and B.

Although the present invention has been specifically described below, the present invention is not limited thereto.

The present invention provides a method for producing eggs having antioxidant activity and blood circulation improving ability.

In one embodiment of the present invention, the first step of preparing a mixed extract comprising sumac extract, pork potato extract, red bean extract and green tea extract; a second step of primary fermenting the mixed extract to obtain a primary fermented product; a third step of secondary fermentation of the primary fermented product to obtain a secondary fermented product; a fourth step of preparing a chicken feed composition comprising the secondary fermented product, soybean meal, wheat, corn and rice bran; a fifth step of feeding the chicken feed composition to chickens; and a sixth step of obtaining eggs from chickens fed with the chicken feed composition; thereby providing a method for producing eggs having antioxidant effects and blood circulation improving effects. 1 shows a brief flow of each step.

In this case, implementing the antioxidant effect may mean that the DPPH radical and ABTS radical scavenging effect are excellent.

In addition, implementing the effect of improving blood circulation may mean that blood circulation is facilitated by inhibiting platelet aggregation to prevent thrombus formation.

(1) a first step of preparing a mixed extract comprising sumac extract, pork potato extract, red bean extract and green tea extract;

In the first step of this embodiment, a mixed extract can be prepared by preparing sumac extract, pork potato extract, red bean extract, and green tea extract, respectively, and mixing them in a specific ratio. In particular, in this embodiment, the sumac extract as the main component, but the following sumac extract, pork potato extract, red bean extract, and green tea extract are included in specific ratios, so that eggs having antioxidant effect and blood circulation improvement effect can be produced. .

The sumac tree ( Rhus verniciflua Stokes ) is a deciduous broad-leaved tree belonging to the Sumacaceae family, and grows to about 12 m in height and 40 cm in diameter. The sumac tree has green shoots and no hairs on the leaves and fruits, and is composed of leaves, flowers, fruits, stem bark, stems, branches, and winter snow. Sumac is effective in improving gastrointestinal diseases, kidney stones, liver disease, osteomyelitis, arthritis, and menstrual irregularities.

The sumac extract is an extract obtained by extraction treatment of sumac tree, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a prepared product or purified product of the extract, or a mixture thereof, using the extract itself and the extract. Extracts of all formable formulations are included. Specifically, the sumac extract of this embodiment may be a liquid from which solids are removed after extraction, but is not limited thereto.

In the sumac extract, the extraction part of the sumac tree may include one or more selected from the group consisting of leaves, flowers, fruits, stem bark, stems, branches, and winter snow.

Extraction of the sumac tree may be performed by any known method, for example, hot water extraction, ethanol extraction, microwave extraction, supercritical extraction, and ultrasonic extraction. can, but is not limited thereto.

The sumac extract is extracted from the sumac tree with an extraction solvent containing at least one selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol. can mean that

Specifically, the sumac extract may be water extracted as an extraction solvent, and more specifically, may be a hot water extract, but is not limited thereto.

The pork potato ( Helianthus tuberosus ) is a perennial herb in the genus Sunflower of the Asteraceae family of the order Asteraceae, and is also called as the calf. The pork potato grows upright to about 1.5 ~ 3 m in height, has rough hairs, and has a gritty surface, and grows wild in the field or in the wild. As it is known that these pork potatoes are effective in diabetes, diet, increase of beneficial intestinal bacteria, and cancer prevention, their intake has been increasing recently.

The pork potato extract is an extract obtained by extraction treatment of pork potatoes, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a prepared or purified product of the extract, or a mixture thereof, such as the extract itself and the extract It includes extracts of all formulations that can be formed using it. Specifically, the pork potato extract of this embodiment may be prepared in the form of a dry powder after extraction, but is not limited thereto.

The extraction of the pork potato may be performed by any known method, for example, hot water extraction, ethanol extraction, microwave extraction, supercritical extraction and ultrasonic extraction by any one method selected from the group consisting of may be, but is not limited thereto.

The pork potato extract is an extraction solvent comprising at least one selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol, the pork potato extract It may mean that it is extracted with

The red bean ( Vigna angularis ) is a dicotyledonous plant, as an annual herb of the Rosaceae legume, also called sodu, jeoksodu, and is a crop mainly cultivated in the East, including China. The red bean contains a large amount of saponin, which is effective for skin beauty and diuretic action, and is good for constipation and digestion because it is rich in dietary fiber and oligosaccharide, and has the effect of improving blood vessel health by clearing blood.

The red bean extract is an extract obtained by extracting red beans, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a prepared product or purified product of the extract, or a mixture thereof, and the like, using the extract itself and the extract. Extracts of all formable formulations are included. Specifically, the red bean extract of this embodiment may be prepared in the form of a dry powder after extraction, but is not limited thereto.

The extraction of the red beans may be performed by any known method, for example, hot water extraction, ethanol extraction, microwave extraction, supercritical extraction, and ultrasonic extraction to be performed by any one method selected from the group consisting of extraction. can, but is not limited thereto.

The red bean extract is extracted with an extraction solvent containing at least one selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol. can mean that

The green tea (Green Tea) is dried so that the leaves of the tea tree give off a blue light as it is, has a good antioxidant effect, lowers the risk of heart disease and cancer, and has a blood sugar control effect. In addition, the green tea is effective in improving liver function, brain, and exercise ability, helps to recover from fatigue, and has a good skin beauty effect, so it is consumed and used in various forms.

The green tea extract is an extract obtained by extracting green tea, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, a prepared or purified product of the extract, or a mixture thereof, using the extract itself and the extract. Extracts of all formable formulations are included. Specifically, the green tea extract of this embodiment may be prepared in the form of a dry powder after extraction, but is not limited thereto.

The extraction of green tea may be carried out by any known method, for example, hot water extraction, ethanol extraction, microwave extraction, supercritical extraction, and ultrasonic extraction to be carried out by any one method selected from the group consisting of extraction. can, but is not limited thereto.

The green tea extract is extracted with an extraction solvent containing at least one selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, and 1,3-butylene glycol. can mean that

In the first step of this embodiment, a mixed extract including the sumac extract, pig potato extract, red bean extract and green tea extract is prepared, and the sumac extract, pig potato extract, red bean extract and green tea extract are 10 to 15: 0.1 to 1 : may be included in a weight ratio of 0.1 to 1:1, in this case, the antioxidant performance and blood circulation improvement performance of the egg prepared according to the present embodiment may be further improved.

Specifically, in the first step of this embodiment, the sumac extract, pig potato extract, red bean extract and green tea extract may be included in a weight ratio of 12-13:0.5-1:0.5-1:1, in which case, this embodiment The antioxidant performance and blood circulation improvement performance of eggs prepared according to the example can be significantly improved.

Most specifically, in the first step of this embodiment, the sumac extract, pig potato extract, red bean extract, and green tea extract may be included in a weight ratio of 12 to 13: 1: 1: 1. In this case, according to this embodiment, The antioxidant performance and blood circulation improvement performance of the prepared egg can be remarkably excellent.

(2) a second step of primary fermentation of the mixed extract to obtain a primary fermented product;

In the second step of this example, the mixed extract obtained in the first step may be first fermented to obtain a fermented primary fermented product.

Specifically, in the second step of this example, by inoculating and culturing a lactic acid bacteria spawn containing Lactobacillus casei in the mixed extract at 0.5 to 1% (v/v), to obtain a primary fermented product In this case, the egg prepared according to the present embodiment may have an antioxidant effect and an effect of improving blood circulation.

In the second step of this embodiment, the primary fermentation may be carried out for 40 to 50 hours at pH: 5 to 6 , temperature: 30 to 40 ℃ conditions. In particular, when the primary fermentation of this embodiment is carried out at the above pH and temperature conditions, when carried out for 40 to 50 hours, the egg prepared according to this embodiment may have an excellent antioxidant effect and blood circulation improvement effect.

If the primary fermentation of this embodiment is carried out for less than 40 hours or more than 50 hours, unlike the case where the primary fermentation is carried out for 40 to 50 hours, the antioxidant effect and blood circulation improvement of the prepared egg The effect may be negligible.

(3) a third step of secondary fermentation of the primary fermented product to obtain a secondary fermented product;

In the third step of this embodiment, the secondary fermentation product may be obtained by secondary fermentation of the primary fermentation product obtained in the second step.

Specifically, in the third step of this example, by inoculating and culturing a lactic acid bacteria seed containing Lactobacillus acidophilus in the primary fermented product at 1 to 2% (v/v), secondary fermentation Water can be obtained, and in this case, the egg prepared according to this embodiment may have an antioxidant effect and an effect of improving blood circulation.

In the third step of this embodiment, the secondary fermentation may be carried out for 10 to 20 hours at pH: 5 to 6 , temperature: 35 to 40 ℃ conditions. In particular, when the secondary fermentation of the present embodiment is carried out at the above pH and temperature conditions, when carried out for 10 to 20 hours, the egg prepared according to the present embodiment may have an excellent antioxidant effect and blood circulation improvement effect.

If, if the secondary fermentation of this embodiment is carried out for less than 10 hours, the secondary fermentation is not sufficiently made, the degree of improvement in the effect may be insignificant. On the other hand, when the secondary fermentation of this embodiment is carried out for more than 20 hours, a large amount of additional components or substances according to the secondary fermentation is generated, and, on the contrary, the antioxidant effect and blood circulation improvement effect of the egg may be reduced.

In particular, in this embodiment, different types of lactic acid bacteria are applied to the primary fermentation and the secondary fermentation, and the fermentation temperature and time are applied differently to maximize the beneficial ingredients generated in each step, according to this embodiment The prepared egg may have an excellent antioxidant effect and blood circulation improving effect.

(4) a fourth step of preparing a chicken feed composition comprising the secondary fermented product, soybean meal, wheat, corn and rice bran;

In the fourth step of this embodiment, a chicken feed composition can be prepared using the secondary fermented product obtained in the third step as an active ingredient.

Specifically, in the fourth step of this embodiment, it is possible to prepare a chicken feed composition comprising the secondary fermented product, soybean meal, wheat, corn and rice bran in a specific weight %. The chicken feed composition according to this embodiment has excellent sensory properties, and the eggs obtained from the chickens specified as the chicken feed composition can implement antioxidant performance and blood circulation improvement performance.

The chicken feed composition of this embodiment includes the secondary fermented product, and the amount of the secondary fermented product is 5 to 20% by weight, specifically 10 to 20% by weight, more specifically 15 to 20% by weight based on the total weight. can be included as In particular, when the chicken feed composition of this example contains 15 to 20% by weight of the secondary fermented product based on the total weight, the ?poomo? Antioxidant performance and blood circulation improvement performance can be realized.

If the chicken feed composition of this embodiment contains less than 5% by weight of the secondary fermented product based on the total weight, the antioxidant performance and blood circulation improvement performance of eggs may be insignificant. On the other hand, when the chicken feed composition of this example contains more than 20% by weight of the secondary fermented product based on the total weight, when it contains 20% by weight or less, and the degree of improvement in the antioxidant performance and blood circulation improvement performance of eggs There is no difference between the two, so economic feasibility is lowered, and sensory characteristics are lowered, so chickens may not eat enough.

The chicken feed composition of this embodiment may include soybean meal, but may contain 10 to 30% by weight, specifically 15 to 30% by weight, and more specifically 20 to 25% by weight of the soybean meal based on the total weight.

If the chicken feed composition of this embodiment contains less than 10% by weight of soybean meal based on the total weight, the nutrient supply to the chickens is not sufficient, and thus the productivity and nutrition of the eggs may be reduced. On the other hand, when the chicken feed composition of the present embodiment contains more than 30% by weight of soybean meal based on the total weight, the preference is lowered, and as a result, egg productivity and nutritional properties may be lowered.

The chicken feed composition of this embodiment may include wheat, but 10 to 30% by weight, specifically 15 to 25% by weight, more specifically 18 to 20% by weight of the wheat based on the total weight.

If the chicken feed composition of this embodiment contains less than 10% by weight of wheat based on the total weight, the nutrient supply to the chickens is not sufficient, and the productivity and nutrition of the eggs may be reduced. On the other hand, when the chicken feed composition of this embodiment contains more than 30% by weight of wheat based on the total weight, the preference is lowered, and as a result, the productivity and nutrition of the eggs may be lowered.

The chicken feed composition of this embodiment includes corn, but may include 7 to 15% by weight of the corn, specifically 8 to 15% by weight, more specifically 9 to 11% by weight based on the total weight.

If the chicken feed composition of this embodiment contains less than 7% by weight of corn based on the total weight, the nutrient supply to the chickens is not sufficient, and the productivity and nutrition of the eggs may be reduced. On the other hand, when the chicken feed composition of this embodiment contains more than 15% by weight of corn based on the total weight, the preference is lowered, and as a result, the productivity and nutrition of eggs may be reduced.

The chicken feed composition of this embodiment includes rice bran, but may include 3 to 10% by weight of the rice bran, specifically 4 to 8% by weight, more specifically 5 to 7% by weight based on the total weight.

If, the chicken feed composition of this embodiment contains less than 3% by weight of rice bran based on the total weight, the nutrient supply to the chickens is not sufficient, the productivity and nutrition of the eggs may be reduced. On the other hand, when the chicken feed composition of this embodiment contains more than 10% by weight of rice bran based on the total weight, the preference is lowered, and as a result, the productivity and nutrition of the eggs may be lowered.

The chicken feed composition of this embodiment may further include all known feed components other than the above components. For example, the chicken feed composition of this embodiment may further include water, vinegar, wood vinegar, rice bran, oyster shell, blood meal, plum powder, natural preservatives, okgluten, garlic powder, and the like, but is not limited thereto.

(5) a fifth step of feeding the chicken feed composition to the chickens; and

In the fifth step of this embodiment, according to the fourth step, the chicken feed composition including the secondary fermented product, soybean meal, wheat, corn and rice bran may be fed to the chickens.

The feeding may be carried out for at least 2 months or more, specifically for at least 3 months or more, more specifically for at least 4 months or more, and the chicken feed composition may be fed in the same amount during the period during which the feeding is carried out. In this case, when the feeding is carried out for less than 2 months, the antioxidant performance and blood circulation improvement performance of the eggs produced according to the present embodiment may be insignificant.

(6) a sixth step of obtaining eggs from chickens fed with the chicken feed composition;

In the sixth step of this embodiment, according to the fifth step, the chicken feed composition of the fourth step may be fed to obtain eggs from the chickens that were fed.

Thereafter, the obtained eggs may be subjected to known post-treatments such as screening, washing, and disinfection.

Eggs produced according to the first to sixth steps described above have excellent consumer preference, and may be effective in improving health functions by showing antioxidant performance and blood circulation improvement performance.

Through the following Examples, Comparative Examples, and Experimental Examples, a method for producing eggs having antioxidant activity and blood circulation improvement ability using a chicken feed composition containing the extract of the present invention as an active ingredient, and eggs produced thereby to explain These examples are only for illustrating the present invention, and therefore, the scope of the present invention is not to be construed as being limited by these examples.

[Example]

Example 1

(1) First step: Preparation of mixed extract

A mixed extract was prepared by mixing sumac extract, pork potato extract, red bean extract, and green tea extract prepared according to the following in Table 1 [unit: weight ratio].

Example 1 sumac extract 5 Potato Potato Extract One red bean extract One green tea extract One

- Preparation of lacquer extract : After drying lacquer tree so that the moisture content is within 15%, cut it into pieces within 10 mm in width and length, and then add purified water about 10 times the dry weight of the dried lacquer tree, and then add it to about 75 The extraction was carried out for about 24 hours while stirring at a temperature of ~ 80 ℃. Then, by filtering the solid content, a liquid sumac extract was prepared. At this time, the extract of the sumac tree was used as a mixture of the same amount of stem bark, stem inside, branch, and leaf.

-Preparation of pork potato extract : Dried pork potatoes so that the moisture content is within 15% are cut into pieces so that the width and length are within 10 mm, and then purified water of about 10 times the dry weight of the dried pork potatoes is added, This was extracted for about 24 hours while stirring at a temperature of about 75 ~ 80 ℃, and then filtered through a filter paper. Then, the solvent of the filtered filtrate was removed using a concentrator, and then freeze-dried to prepare a powdered pork potato extract.

- Preparation of red bean extract : To the dried red beans so that the moisture content is within 15%, purified water of about 10 times the dry weight of the red beans was added, and extraction was carried out for about 24 hours while stirring at a temperature of about 75 to 80 ° C. Then, it was filtered with filter paper. Then, the solvent of the filtered filtrate was removed using a concentrator, and then freeze-dried to prepare a powdery red bean extract.

- Preparation of green tea extract : After crushing the dried green tea finely, purified water of about 10 times the dry weight of the dried green tea is added, and the extraction is carried out for about 24 hours while stirring at a temperature of about 55 to 60 ° C., Filtered with filter paper. Then, the solvent of the filtered filtrate was removed using a concentrator, and then freeze-dried to prepare a green tea extract in powder form.

(2) Second stage: primary fermentation

The mixed extract is inoculated with 0.5 to 1% (v/v) of lactic acid bacteria seed bacteria based on the mixed extract (concentration: about 2 × 10 ⁵ cfu/ml), pH: 5 to 6 , temperature: 33 to 35 ℃ condition , by performing aerobic culture for 40 to 50 hours to perform secondary fermentation, a primary fermentation product was obtained.

At this time, for culture, 5 g/L of a carbon source (glucose) and 5 g/L of a nitrogen source (soipeptone) were added together, and stirred at regular time intervals so that each component could be mixed evenly.

As the lactic acid bacteria spawn, Lactobacillus casei was used.

(3) 3rd stage: secondary fermentation

Inoculate the primary fermented product with 1.5 to 2% (v/v) of lactic acid bacteria seed bacteria based on the primary fermented product (concentration: about 2 × 10 ⁵ cfu/ml), pH: 5 to 6 , temperature: 37 to After performing secondary fermentation by aerobic culture at 40 ° C. for 10 to 20 hours, after completion of the reaction, sterilization was performed by heating at about 100 to 140 ° C. for about 15 minutes, finally obtaining a secondary fermented product .

At this time, for culture, 5 g/L of a carbon source (glucose) and 5 g/L of a nitrogen source (soipeptone) were added together, and stirred at regular time intervals so that each component could be mixed evenly.

As the lactic acid bacteria seed, Lactobacillus acidophilus was used.

(4) 4th step: production of chicken feed composition

Based on the total weight of the chicken feed composition, 20% by weight of secondary fermentation product, 20% by weight of soybean meal, 20% by weight of wheat, 10% by weight of corn, 5% by weight of rice bran and the remaining amount of water were mixed to prepare a chicken feed composition.

(5) Step 5: Feeding

The chicken feed composition was fed to chickens 3 times a day for breakfast, lunch and dinner for 4 months, and all were fed in the same amount.

(6) 6th step: obtaining eggs

After the chickens were fed the chicken feed composition for 4 months, the eggs laid by the chickens were collected, washed and disinfected, and finally eggs were obtained.

Examples 2 to 5

Eggs were produced in the same manner as in Example 1, except that a mixed extract in which sumac extract, pork potato extract, red bean extract and green tea extract were mixed in the ratio of Table 2 [unit: weight ratio] was prepared and used.

In addition, the amounts of each of the mixed extracts according to Examples 1 to 5 used in the process of producing eggs were all the same.

At this time, in Table 2, the ratio of the mixed extract of Example 1 is also indicated.

Example 1 Example 2 Example 3 Example 4 Example 5 sumac extract 5 10 13 15 20 Potato Potato Extract One One One One One red bean extract One One One One One green tea extract One One One One One

[ Comparative Example ]

Comparative Examples 1 to 5

Eggs were produced in the same manner as in Example 1, except that a mixed extract containing only the components marked with '○' in Table 3 was prepared and used.

At this time, the components (marked ○) used in Table 3 were all used in the same amount.

Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 sumac extract ○ × × ○ × Pork Potato Extract ○ ○ × ○ ○ red bean extract × ○ ○ ○ ○ green tea extract × × ○ × ○

Comparative Example 6

Eggs were produced in the same manner as in Example 1, except that primary fermentation was not performed.

Comparative Examples 7 to 8

Eggs were produced in the same manner as in Example 1, except that the time conditions of Table 4 [unit: time] were performed for the primary fermentation.

Comparative Example 7 Comparative Example 8 1st fermentation time 30 to 35 55 to 60

Comparative Example 9

Eggs were produced in the same manner as in Example 1, except that secondary fermentation was not performed.

Comparative Examples 10 to 11

Eggs were produced in the same manner as in Example 1, except that the secondary fermentation was performed under the time conditions of Table 5 [unit: time].

Comparative Example 10 Comparative Example 11 Second fermentation time 1 to 3 25 to 30

Comparative Examples 12 to 19

Eggs were produced in the same manner as in Example 1, except that a chicken feed composition prepared by mixing each component according to Table 6 [unit: wt%] was used.

comparative example
12 comparative example
13 comparative example
14 comparative example
15 comparative example
16 comparative example
17 comparative example
18 comparative example
19 secondary fermentation One 25 20 20 20 20 20 20 Soybean Meal 20 20 5 35 20 20 20 20 wheat 20 20 20 20 5 35 20 20 corn 10 10 10 10 10 10 One 20 rice bran 5 5 5 5 5 5 5 5 water to.100

[Experimental example]

Experimental Example 1: Evaluation of antioxidant performance

(1) Evaluation of DPPH radical scavenging activity

The egg of Example 1 was mixed so that the yolk and the white were uniform, and this was used as a sample to evaluate the DPPH radical scavenging activity. Specifically, DPPH radical scavenging activity (Electron donating abilities, EDA) using the sample of Example 1 was measured as follows.

To 100 μl of each sample solution, 50 μl of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) was added, stirred, and then left to stand for 30 minutes after blocking the light, and absorbance was measured at 517 nm. The antioxidant performance of the sample was evaluated as an IC50 (half maximal inhibitory concentration) value indicating the concentration showing the intermediate activity of radical scavenging activity, and the average value of three times is shown in Table 7 [unit: mg/ml].

The IC50 refers to a concentration of a value that reduces the absorbance of the DPPH radical by 50%.

Antioxidant performance was evaluated using the eggs of Examples 2 to 5 and Comparative Examples 1 to 19 in the same manner as in Example 1, and the results are shown in Table 7.

IC50 IC50 Example 1 19.00 Comparative Example 8 40.36 Example 2 14.56 Comparative Example 9 62.74 Example 3 8.24 Comparative Example 10 58.99 Example 4 15.78 Comparative Example 11 51.05 Example 5 18.99 Comparative Example 12 78.05 Comparative Example 1 30.24 Comparative Example 13 19.54 Comparative Example 2 29.14 Comparative Example 14 25.60 Comparative Example 3 31.00 Comparative Example 15 24.84 Comparative Example 4 40.01 Comparative Example 16 27.60 Comparative Example 5 32.48 Comparative Example 17 24.44 Comparative Example 6 54.99 Comparative Example 18 26.58 Comparative Example 7 45.98 Comparative Example 19 27.21

Referring to Table 7, it can be seen that, according to the present example, antioxidant performance is realized, and better antioxidant performance is realized than that of the comparative example not according to this example.

(2) Evaluation of ABTS radical scavenging ability

7 mM 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and 2.4 mM potassium persulfate were mixed and left in a dark place at room temperature for 24 hours to form ABTS+, diluted with ethanol and carried out in 100 μl of ABTS+ After adding 100 μl of the sample of Example 1 and leaving it for 1 minute, absorbance was measured at 732 nm.

The antioxidant performance of the sample was evaluated as an IC50 (half maximal inhibitory concentration) value indicating the concentration showing the intermediate activity of the radical scavenging ability, and the average value of three times is shown in Table 8 [unit: mg/ml].

Antioxidant performance was evaluated using the eggs of Examples 2 to 5 and Comparative Examples 1 to 19 in the same manner as in Example 1, and the results are shown in Table 8.

IC50 IC50 Example 1 21.02 Comparative Example 8 42.56 Example 2 16.58 Comparative Example 9 64.77 Example 3 10.27 Comparative Example 10 58.91 Example 4 17.77 Comparative Example 11 53.35 Example 5 20.10 Comparative Example 12 80.25 Comparative Example 1 32.44 Comparative Example 13 21.64 Comparative Example 2 31.15 Comparative Example 14 27.55 Comparative Example 3 33.00 Comparative Example 15 26.87 Comparative Example 4 42.81 Comparative Example 16 29.80 Comparative Example 5 34.45 Comparative Example 17 27.46 Comparative Example 6 57.38 Comparative Example 18 27.99 Comparative Example 7 46.99 Comparative Example 19 29.95

Referring to Table 8, it can be seen that, according to the present example, antioxidant performance is realized, and better antioxidant performance is realized than that of the comparative example not according to this example.

Experimental Example 2: Blood circulation improvement performance evaluation

(1) Evaluation of the ability to inhibit platelet aggregation

In order to separate human platelet rich plasma (PRP), 3.2% sodium citrate was used as an anticoagulant, and blood was collected from the veins of a healthy male who had not taken the drug for more than 2 weeks.

After centrifuging 150 g of the collected blood for 15 minutes, a supernatant (PRP) was obtained and the residue was centrifuged again to separate platelet poor plasma (PPP). The number of platelets in the separated PRP was counted using a cell counter, and after diluting the PRP with PPP so that 3 x 10 ⁸ platelets were included in 1 ml, it was used in the experiment.

Platelet aggregation activity was measured by changing the absorbance using a platelet aggregometer (lumi-aggregometer, Chrono-Log Co., USA).

Specifically, a concentration of 200 mg/ml of the sample of Example 1 was added to the PRP pre-cultured in a thermomixer so as to be about 37° C. for 2 minutes, and incubated for 7 minutes. 500 μl of the cultured PRP was placed in a silicone-coated cuvette for a platelet aggregation system and incubated for 3 minutes. Then, 1-3 μg/ml, the minimum concentration for maximal aggregation, was added to the platelet aggregation-inducing sample, collagen, for 6 minutes. The reaction was observed, the platelet aggregation inhibition rate (%) was evaluated, and the average value of 5 times is shown in Table 9.

At this time. The platelet aggregation inhibition rate (%) in Table 9 shows a comparative value when the platelet aggregation inhibition rate of the control group treated only with collagen was set to 0%.

In the same manner as in Example 1, using the eggs of Examples 2 to 5 and Comparative Examples 1 to 19, platelet aggregation inhibition rate was evaluated.

Platelet aggregation inhibition rate (%) Platelet aggregation inhibition rate (%) Example 1 40.0 Comparative Example 8 21.7 Example 2 48.7 Comparative Example 9 12.4 Example 3 55.7 Comparative Example 10 15.9 Example 4 47.4 Comparative Example 11 25.4 Example 5 42.5 Comparative Example 12 11.8 Comparative Example 1 21.8 Comparative Example 13 38.0 Comparative Example 2 20.9 Comparative Example 14 30.4 Comparative Example 3 23.4 Comparative Example 15 35.8 Comparative Example 4 25.0 Comparative Example 16 32.6 Comparative Example 5 21.7 Comparative Example 17 34.0 Comparative Example 6 10.5 Comparative Example 18 30.5 Comparative Example 7 16.4 Comparative Example 19 32.7

Referring to Table 9, it can be seen that the eggs of Examples 1 to 5 produced according to this Example exhibited a platelet aggregation inhibitory effect, and were superior to the eggs of Comparative Examples 1 to 19 not according to this Example. Able to know.

That is, the eggs produced according to the present embodiment have the effect of inhibiting blood clots, which means that they show the effect of improving blood circulation.

As described above, the description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can easily transform into other specific forms without changing the technical spirit or essential features of the present invention. You will understand. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the following claims rather than the above detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be interpreted as being included in the scope of the present invention. do.

Claims (9)

A first step of preparing a mixed extract comprising sumac extract, pork potato extract, red bean extract and green tea extract;
a second step of primary fermenting the mixed extract to obtain a primary fermented product;
a third step of secondary fermentation of the primary fermented product to obtain a secondary fermented product;
a fourth step of preparing a chicken feed composition comprising the secondary fermented product, soybean meal, wheat, corn and rice bran;
a fifth step of feeding the chicken feed composition to chickens; and
A method for producing eggs having antioxidant activity and blood circulation improving ability, comprising; a sixth step of obtaining eggs from chickens fed with the chicken feed composition.
The method of claim 1,
The mixed extract is
A method for producing eggs having antioxidant and blood circulation improving properties, comprising the sumac extract, pork potato extract, red bean extract and green tea extract in a weight ratio of 10-15:0.1-1:0.1-1:1 .
The method of claim 1,
The first fermentation
Method for producing eggs having antioxidant activity and blood circulation improving ability, characterized in that the mixed extract is inoculated with lactic acid bacteria spawn containing Lactobacillus casei at 0.5 to 1% (v/v) .
4. The method of claim 3,
The first fermentation
pH: 5 to 6 , temperature: 30 to 40 ℃ conditions, characterized in that carried out for 40 to 50 hours, a method for producing eggs having antioxidant activity and blood circulation improving ability.
The method of claim 1,
The second fermentation
It is characterized in that the primary fermentation is carried out by inoculating a lactic acid bacteria seed containing Lactobacillus acidophilus in an amount of 1 to 2% (v/v), having antioxidant and blood circulation improving ability. Methods of production of eggs.
6. The method of claim 5,
The second fermentation
pH: 5 to 6 , temperature: 35 to 40 ℃ conditions, characterized in that carried out for 10 to 20 hours, a method for producing eggs having antioxidant activity and blood circulation improving ability.
The method of claim 1,
The chicken feed composition is
Antioxidant, characterized in that it contains 5 to 20% by weight of secondary fermented product, 10 to 30% by weight of soybean meal, 10 to 30% by weight of wheat, 7 to 15% by weight of corn, and 3 to 10% by weight of rice bran based on the total weight A method for producing eggs having the ability to improve performance and blood circulation.
The method of claim 1,
in the fifth step
A method for producing eggs having antioxidant activity and blood circulation improving ability, characterized in that feeding the chicken feed composition to chickens for at least 2 months or more.
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