KR20130042322A - Feed additives and manufacturing method thereof and production method of egg containing saponin - Google Patents

Abstract

PURPOSE: A method for manufacturing a feed additive and a method for producing eggs containing saponin are provided to promote growth of livestocks and immunity, and to produce the functional eggs containing saponin. CONSTITUTION: A method for manufacturing a feed additive comprises: a step of primarily fermenting a mixture containing Codonopsis lanceolata and/or Platycodon grandiflorum, and Humulus japonius; and a step of mixing the fermented mixture, and secondly fermenting to prepare the feed additive. A feed composition is prepared by mixing 0.1-10 parts by weight of the feed additive based on 100 parts by weight of formula feed. Eggs containing saponin are produced by feeding the feed composition to a layer chicken. The mixture further contains Perilla frutescens, Impatiens balsamina, Salvia plebeia, loofah, Saururus Herba, Houttuynia cordata, Cudrania tricuspidata, onion, Foeniculi fructus, Eucommia ulmoides, Hovenia dulcis, Acanthopanacis cortex, Japanese apricot, garlic, kelp, green tea, and leaf mustard.

Description

Feed additives and preparation method thereof and production method of egg containing saponin {Feed additives and manufacturing method eg and production method of egg containing saponin}

The present invention relates to a feed additive, a method for producing the same, and a method for producing an egg containing saponin. More specifically, a saponin-containing plant and saponin-containing plant, such as deodeok and bellflower, can be produced using saponin-containing eggs. The present invention relates to a feed additive, a method for preparing the same, and a method for producing an egg containing saponin.

Eggs are nutritionally valuable foods that are balanced and contain abundant nutrients such as unsaturated fatty acids, iron and vitamins, including high quality protein (Forsyhe 1970, The Bakers Digest 8, 4-46).

As consumers' health-oriented consciousness rises, egg transfer of specific ingredients in feed relative to eggs (Park et al. 2005, Kor J Poult Sci 32 (4), 269-273; Kim et al. 2002, J Kor Soc Food Sci) Nutr 31, 373-377; Kang et al. 1994, Kor J Poult Sci 21, 227-237) and ingredient analysis of brand eggs (Han et al. 1999, Kor J Anim Sci 41, 343-354) and egg antibody production (Baek & Bae 1999, J Life Resource & Industry 4, 1-6).

Recently, functional eggs have been produced that increase the added value of eggs, such as increasing hygiene, freshness and nutritional value. These functional eggs are marketed as special eggs in the market and are distributed with differentiation from conventional eggs.

Functional eggs can be divided into four types depending on the type of ingredients to be fortified. Eggs are enriched with nutrients that are easy to be lacking in the human body, that is, nutrients such as iodine, vitamin-enhanced eggs, and omegarans. Second, eggs with physiologically active substances other than nutrients include taurine and L-carnitine-containing eggs. Such functional reinforcement eggs have been controversial due to the lack of scientific evidence and analytical technology. The third is an immune antibody-enhanced egg that is fortified with an immune antibody, for example, IgY eggs. Lastly, low egg content lowers harmful ingredients in the human body, which reduces consumers' purchase motivation, and low-cholesterol eggs that lower cholesterol content in eggs are typical.

The development of such a conventional functional egg was made in the direction of strengthening the ingredients beneficial to the human body to the unique ingredients of natural eggs, or lowering the harmful ingredients of the human body among the unique ingredients of eggs. However, in addition to simply adding or reducing specific ingredients in eggs, it is also possible to develop more differentiated functional eggs by strengthening specific ingredients in the eggs and linking the functions of these specific ingredients with the nutritional properties of the eggs. need.

The present invention aims to provide a feed additive, a method for producing the same, and a method for producing an egg containing saponin, which can produce a functional egg containing saponin at the same time as it promotes the growth and immunity of livestock at the time of feeding. .

Feed additive of the present invention for achieving the above object is characterized in that it comprises at least any one selected from Deodeok and bellflower, fermented product fermented hwansam vine.

The feed additive is characterized in that it further comprises fermented fermented perilla, balsam, Morel cabbage, loofah.

The feed additive is characterized in that it further comprises a silver colloid.

And the method of producing a feed additive of the present invention for achieving the above object is a first step of fermenting at least any one selected from Deodeok and bellflower, and a mixture comprising hwansam vine; And a second step of mixing and fermenting the second herbal medicine with the fermented product fermented in the first step.

The first step is characterized in that the mixture is mixed with elvan, sugar, water, makgeolli and first fermented by storing at 20 to 30 ℃ for 4 to 8 months.

The mixture is characterized in that it further comprises perilla, balsam, Morel cabbage, scrubber, triticale, eoseongcho, kkujippong, onion, fennel, tofu, hut, ogapi, plum, mugwort, radish, garlic, kelp, green tea, lampshade .

In the second step, the herbal medicine is Gilgil, lobules, fennel, pogongyeong, four ginseng, larvae, gold, characterized in that fermented by further mixing the medicinal herb bark, ganban stone, sugar, hut tree extract, silver colloid.

And a method for producing an egg containing saponin for achieving the above object comprises the first step of blending 0.1 to 10 parts by weight of the feed additive with respect to 100 parts by weight of the feed compound to obtain a feed composition; And feeding the feed composition to a laying hen to produce an egg containing saponin.

As described above, according to the present invention, it is possible to produce functional eggs containing saponin by feeding the chickens while improving the productivity by increasing the growth and immunity of the livestock.

In addition, the eggs produced are excellent in antioxidant activity can maintain the freshness for a long time can increase the shelf life.

Hereinafter, a feed additive, a method for preparing the same, and a method for producing an egg containing saponin according to a preferred embodiment of the present invention will be described in detail.

Feed additive according to an embodiment of the present invention includes at least any one selected from Deodeok and bellflower, and fermented product fermented hwansamvine.

In the present invention, deodeok and bellflower are used as a source of saponin contained in eggs. Feed additive of the present invention may include only one or both of the deodeok and bellflower. Deodeok and bellflower use roots. Preferably, the deodeok and bellflower are used directly collected from the dried state.

Codonopsis lanceolata is a perennial vine plant belonging to Campahutaceae and is known for its immune and anticancer effects. As an example, Korean Patent Laid-Open Publication No. 2003-57317 describes that it may contribute to the enhancement of nourishing and tonic effects through the proliferation of lymphocytes and regulation of NO in macrophages, iNOS gene expression and cytokine expression. , Korean Unexamined Patent Publication No. 2008-12812 disclose that hot water extract and ethanol extract of Deodeok have cancer cell proliferation inhibitory effect. Deodeok is known to contain bioactive ingredients such as Saponin, Inulin, Phytoderin, Leoithin, and Pentosan. The efficacy of saponins contained in deodeok has been reported on anti-inflammatory effects (Triterpenoid saponins with anti-inflammatory activity from Codonopsis lanceolata. 2008 Sep; 74 (11): 1412-5. Epub 2008 Jul 29).

And bellflower ( Platycodon grandiflorum ) is a perennial plant belonging to the family of languidaceae. The major bioactive substances of bellflower are 10 kinds of saponins, such as Platicodion-A, -C, -D1, -D2, Platicodigenin, Polygalacin-D1, -D2 Glycosides and platycodins, but also include betulin, inutin, phytosterol, and the like. Saponins contained in bellflower are antitussive, expectorant, central nervous inhibitory (sedative, analgesic, antipyretic), anti-inflammatory against acute and chronic inflammation, anti-ulcer and gastric secretion, anticholinergic to lower blood pressure by expanding blood vessels Action, hypoglycemic activity, cholesterol metabolism improvement (Toshiyuki Akiyama et al., Chem. Pharm. Bull., 20, 1952 (1972); Akihito Tada et al., Chem. Pharm. Bull., 23, 2965 (1975); Hiroshi Ishii et al., J. Chem. Soc., Perkintrans I, 661 (1984); Eun Bang Lee, J. Pharm. Soc.Kor., 19, 164 (1975).

Humulus japonicus ) is a vine of the family Ginza, also called Yulcho. Hwansam vine is known to have the function of eliminating fish blood, lowering blood pressure, and eliminating toxicity. In Oriental medicine, fruit is prescribed as a high-density agent (苦味 健胃 劑), and outpost is diuretic (利尿劑), and stem is a raw material of fiber. Used.

In the present invention, hwansam vine uses all of the roots, leaves, stems, flowers outpost. Hwansam vine is expected to be able to maintain the freshness of eggs for a long time because of the excellent antibacterial and anti-oxidant activity, resulting in increased immunity of livestock.

Feed additives of the present invention is expected to be able to increase the useful components by microbial metabolism by using at least one selected from the deodeok and bellflower, and fermented fermentation form of ginseng vine. In particular, through the fermentation saponin contained in the deodeok and bellflower can be broken down into a non-glycoside form of low molecular weight to facilitate the absorption in the body. Saponins are glycosides composed of glycosides and aglycones and are widely distributed in the plant system. They are not absorbed by the body without being degraded by the unique microorganisms in the intestine.

Feed additive of the present invention may further include fermented fermented perilla, balsam, Morel cabbage, scrubber.

Perilla frutescens is an annual herb belonging to the Lamiaceae family, with hairs on the leaf veins and thin hairs on the back. In herbal medicine, the leaves of perilla are called jaso (紫蘇), and the seeds are called soja. It is mainly used for the treatment of skin diseases such as sweating, hemostasis, antipyretic, analgesic and atopy. The perilla contains about 0.5% essential oil in the outpost, and about 55% of the essential oil is known as phenyl aldehyde.

Impatiens balsamina ) has long been known for its clinical efficacy in folk medicine. Balsam, for example, has been used for constipation, egg mass, planting, fish poisoning, blood loss, anti-inflammatory pain and arthritis, menstrual irregularities, bruises, detoxification, and the like. It is also known to have antibacterial and pathogenic fungi.

Salvia plebeia is a perennial herb of Lamiaceae. It is also called persimmon, blue and white, mamacho, dongdongcheong, and cat. Morel cabbage has a vivid aroma, tastes spicy and bitter, cool and nonpoisonous, and is known to be particularly effective for asthma. Ingredients are known to contain phenolic substances, essential oils, saponins, cardiac glycosides, unsaturated fatty acids, etc., in addition to flavonoids such as homoplantagen, hispiduline, eucaponolin, eucaponolin-7-glucoside and the like.

Scrubber ( Lufa cylindrica ) is an annual herbaceous herbaceous plant of the gourd. Its main components are moisture, ash, protein, carotene, dietary fiber, calcium, potassium, iron, magnesium, sodium, and many nutrients, including 17 essential amino acids. It is contained.

In addition, the feed additive of the present invention may further include a silver colloid. The antibacterial effect of silver in silver colloid prevents the growth of harmful bacteria. Silver colloid can use a commercially available product or what was manufactured by the conventional silver colloid manufacturing technique.

Hereinafter, a description will be given of a method of preparing the above-described feed additive.

First, a primary fermentation product is obtained by primary fermentation of a mixture of at least one selected from deodeok and bellflower, and ginseng vines.

As an example, the primary fermentation product is obtained by fermenting a mixture of bellflower and panax ginseng. For the first fermentation, the bellflower and hwansam vine are cut into small pieces, mixed and put into a container. At this time, it is blended with bellflower 20-40 parts by weight based on 100 parts by weight of hwansam vine. For fermentation of the mixture, after addition of ganbanite powder, sugar, water and makgeolli, it is stored at 20 to 30 ° C. for 4 to 8 months to ferment. In the present invention, since the fermentation proceeds by the microorganisms contained in makgeolli, it is not necessary to artificially inoculate specific strains.

The primary fermentation may be fermented by further mixing the perilla, balsam, Morel cabbage, loofah. In addition, it may be fermented by mixing more than three hundred seconds, eoseongcho, kkujippong, onions, fennel, tofu, hut, ogapi, plum, wormwood, radish, garlic, kelp, green tea, freshly. In addition, ginger, wolfberry, lacquer can be further mixed and fermented.

As an example of the first fermentation, 20 to 40 parts by weight of bellflower, 100 to 150 parts by weight, 300 to 200 parts by weight, 65 to 85 parts by weight of fish vinegar, 40 to 60 parts by weight of zigzag, 30 to 35 parts by weight of perilla, 400 to 600 parts by weight of onions, fennel 25 to 35 parts by weight, worms 65 to 85 parts by weight, lumber tree 45 to 55 parts by weight, ogapi 100 to 150 parts by weight, plums 30 to 40 parts by weight, mugwort 100 to 150 parts by weight, Balsam 90 to 110 parts by weight, radish 85 to 95 parts by weight, garlic 160 to 190 parts by weight, seaweed 1 to 9 parts by weight, seaweed cabbage 35 to 40 parts by weight, green tea 35 to 40 parts by weight, fresh 200 to 250 parts by weight, 100 to 150 parts by weight of loofah, 15 to 25 parts by weight of ganban stone, 2000 to 2500 parts by weight of sugar, 10000 to 13000 parts by weight of water, 400 to 600 parts by weight of rice wine, and stored in a container at 20 to 30 ℃ for 4 to 8 months Primary fermentation.

After the primary fermentation is completed, the contents may be taken out of the container and filtered to obtain a liquid primary fermentation product.

Next, by mixing the herbal medicine to the primary fermentation and secondary fermentation to obtain a secondary fermentation. As a Chinese herbal medicine, Gilgyeong, lobules, fennel, Pogongyoung, Sasam, Gaza, and Gumja are used. Chinese herbal medicine is ground in a blender and used in powder form.

Secondary fermentation can be fermented by further mixing the Chinese medicine with wheat flour, ganban stone powder, sugar, hawthorn extract, and silver colloid. In addition, it is possible to further mix the herbal by-products that are herbal remnants that occur after the manufacture of decoction.

As an example of the second fermentation, 50 to 130 parts by weight of the herbal medicine, 1000 to 3000 parts by weight of herbal by-products, 500 to 1500 parts by weight of wheat vegetation, 300 to 360 parts by weight of ganban stone, and 23 to 43 weight of sugar based on 100 parts by weight of the first fermentation product Part, 1 to 7 parts by weight of the extract of the larvae, 10 to 20 parts by weight of the silver colloid is mixed and stored in a container for 10 to 20 days at 20 to 30 days of fermentation. In the secondary fermentation process, fermentation proceeds by the microorganisms contained in the primary fermentation product.

Through the secondary fermentation process described above, the feed additive of the present invention is prepared. Feed additive means a substance added in a small amount to the feed for nutritional or specific purposes, and the feed additive of the present invention may be fermented by itself or known feed additives. . If known feed additives are added, the fermentation may be applied 10 to 90% by weight of the total weight of the feed additive.

Feed additives of the present invention are fed to a livestock by mixing in a predetermined ratio with a conventional blended feed. For example, 0.1 to 10 parts by weight of the feed additive may be added to 100 parts by weight of the blended feed. The type of compound feed may be appropriately selected according to the type of livestock such as chicken, cow, pig, or duck.

Feed composition containing 0.1 to 10 parts by weight of feed additives based on 100 parts by weight of chicken feed may be fed to a laying hen to produce eggs containing saponin. 110 to 120 days after hatching, the feed composition is fed to the laying hens that start acetic acid for 20 to 40 days to breed. If the feed is less than 20 days, the content of saponin contained in the egg is insignificant, and if fed more than 40 days, the increase in the content of saponin contained in the egg is not large compared to the feeding period.

Hereinafter, the present invention will be described through examples. However, the following examples are intended to illustrate the present invention in detail, and the scope of the present invention is not limited to the following examples.

(Example)

Hwansamvine 4kg, bellflower 1.2kg, three hundred seconds 7kg, Eoseongcho 3kg, kkujippong 2kg, perilla 1.3kg, onion 20kg, fennel 1.2kg, tofu 3kg, hut 2kg, cucumber 5kg, plum 1.4kg, wormwood 5kg, balsam 4kg, radish 3.2 kg, Garlic 7kg, Kelp 200g, Morel cabbage 1.5kg, Green tea 1.5kg, Fresh 9kg, Scrubbing brush 5kg, McBan Stone Co., Ltd. 20kg, Sugar (Q1, Samyangsa) 90kg, Water 450kg, Makgeolli (Samyang Makgeolli) 20 kg of the mixture was added to the container and then stored at 25 ° C. for 6 months to ferment the first. After the first fermentation, the solids were filtered using a sieve to obtain a liquid primary fermentation product.

For 6kg of primary fermentation, 5.4kg of herbal medicine, 120kg of herbal by-product, 60kg of wheat bran, 20kg of ganban stone powder, 2kg of sugar, 2kg of walnut tree extract, 220g of silver colloid and 1kg of silver colloid were mixed and stored at 25 ℃ for 15 days. Secondary fermentation produced a feed additive.

Herbal medicine used during the secondary fermentation is composed of a mixture of pulverized gilyeong 1.2kg, lobule 600g, fennel 1.2kg, pogongyeong 600g, four ginseng 600g, 600g of barberry, 600g golden, the bark extract is weight ratio of water on the bark tree 10 times was added and heated at 100 degreeC for 6 hours, and the solid was filtered out and prepared. And leftovers after decoction of Chinese medicine with Yeongyo, Cheonggung, Shouo, Baeksunpi, Dongguk, Gyeji, Baekjak, Hwangnyeon, Iris, Carver, Jooksil, Hyeonggae, Safflower, Rhubarb, and licorice with the same weight ratio. It was used and finely prepared by grinding. The silver colloid was prepared by pouring 1 liter of distilled water into a silver nano water maker (Coco Silver 500, Coco Silver) equipped with silver rod electrodes and then electrolyzing.

Chicken breeding experiment

Experimental experiments were conducted using 100 animals of 16-week-old Lohmann Brown laying hens as experimental animals. Feed and water were free-vegetable, and other feeding was carried out for 6 weeks in accordance with domestic laying management practices. During the experimental period, the feed containing 20 kg of the feed additive of the above example was fed per 1 ton of the compound feed for laying hens (Initial Spawning 3, Cargill Agripurina, Korea). Five weeks after the start of the experiment, samples were taken from the eggs laid from the laying hens, and the test results were analyzed for saponin components. The results are shown in Table 1 below.

Test Items Irradiation Ponine (mg / g) sample 41.8354

As shown in Table 1, the result of component analysis of the egg was found to contain about 42 mg of irradiated per g. Considering that conventional eggs do not contain irradiated saponin, it can be seen that the eggs produced by feeding the feed additive of the present invention contain a large amount of saponin.

On the other hand, the results of measuring the radical scavenging activity of the sample to examine the antioxidant activity of the eggs are shown in Table 2 below. The test sample was used for the eggs produced by the breeding experiment, the control sample was fed to the compound feed to produce the eggs in the same manner as the breeding experiment but no feed additives.

The antioxidant donation ability (EDA%) of the samples was measured by modifying Blois' method. 0.5 mL of 0.2 mM DPPH (1,1-diphenyl-2-picryl hydrazyl) was added to 2 mL of the sample solution, stirred for 30 minutes, and the absorbance was measured at 517 nm. The electron donating effect was expressed as the absorbance reduction ratio of the sample solution and the no-added solution. Radical scavenging activity was calculated by the following equation.

Radical scavenging activity (%) = (1-absorbance of sample added / absorbance of non-added sample) × 100

division Radical scavenging performance Test Sample 34.7 ± 0.65 Control sample 12.4 ± 0.40

Looking at the results of Table 2, the test sample showed a much higher value than the 12.4 ± 0.40 scavenging ability of the control sample. As a result, the eggs produced by feeding the feed additive of the present invention are excellent in antioxidant activity and can be expected to be able to maintain the freshness of the eggs for a long time, thereby increasing storage.

In the above, the present invention has been described with reference to one embodiment, which is merely exemplary, and it will be understood by those skilled in the art that various modifications and equivalent embodiments are possible.

Accordingly, the true scope of protection of the present invention should be determined only by the appended claims.

Claims (8)

At least one selected from the deodeok and bellflower, and feed additives, characterized in that it comprises a fermented product fermented hwansam vine. The feed additive of claim 1, wherein the feed additive further comprises a fermentation product of fermented perilla, balsam, Morel cabbage, and loofah. The feed additive of claim 1, wherein the feed additive further comprises a silver colloid. A first step of first fermenting the mixture comprising at least one selected from deodeok and bellflower, and ginseng vine;
And a second step of mixing the fermented product fermented in the first step and second fermentation. The method of claim 4, wherein the first step of mixing the mixture with ganban stone, sugar, water, makgeolli is stored for 4 to 8 months at 20 to 30 ℃ primary fermentation method characterized in that the fermentation. The method of claim 4, wherein the mixture is perilla, balsam, Morel cabbage, loofah, three hundred seconds, eoseongcho, kkujippong, onion, fennel, tofu, walnut, ogapi, plum, mugwort, radish, garlic, kelp, green tea, freshly Method for producing a feed additive, characterized in that it comprises a. The method of claim 4, wherein in the second step, the herbal medicine is Gilgil, lobules, fennel, pogongyeong, four ginseng, individuality, gold,
The method of producing a feed additive, characterized in that the fermented by further mixing the medicinal herbs, barley, sugar, hut extract, silver colloid. A first step of formulating a feed composition by mixing 0.1 to 10 parts by weight of the feed additive according to any one of claims 1 to 3 with respect to 100 parts by weight of the blended feed;
And a second step of producing the egg containing saponin by feeding the feed composition to the laying hens.