CN103609909B - Pollution-free aquatic feed additive using ginkgo leaves as base stock - Google Patents
Pollution-free aquatic feed additive using ginkgo leaves as base stock Download PDFInfo
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Abstract
The invention relates to a pollution-free aquatic feed additive using ginkgo leaves as a base stock. The pollution-free aquatic feed additive is prepared from the following materials in parts by weight: 150-200 parts of ginkgo leaves, 100-120 parts of potatoes, 30-50 parts of lotus leaves, 15-20 parts of common andrographis herbs, 8-12 parts of Chinese mosla herbs, 5-7 parts of Chinese rhubarb, 7-12 parts of mangnolia officinalis, 3-5 parts of negundo chastetree fruits, 50-80 parts of chlorella powder, 50-80 parts of egg powder, 5-10 parts of medical stones and 20-30 parts of corn starch. The feed additive disclosed by the invention completely adopts natural components, is not extracted and treated by any harmful chemical reagent and pollution-free to an aquaculture environment, and completely satisfies green, safe and pollution-free requirements of modern aquaculture industry.
Description
Technical field
The invention belongs to field of fodder, relating to a kind of take ginkgo leaf as the nuisanceless feeding additive aquatic animal of base-material.
Background technology
In recent years, along with expanding economy, the living standard of people progressively improves, and entire society also progressively improves the demand of protein in food, along with the development of fishery and animal husbandry, the supply of meat can satisfy social needs substantially, particularly oppresses, as a kind of plain boiled pork, fat content is low, commercially receive liking of broad masses of the people, but qualitatively, also have certain distance apart from the expectation of people.Still not high in the degree of China's culture fishery scale, be main mainly with the fishing ground of middle and small scale and family's breed in stew, mainly adopt traditional method for breeding.But some problems of existence more or less in traditional breeding process, such as in carp culture process, most raiser cultivates idea and falls behind, feed management does not catch up with, and a lot of raiser adopts low material, its energy and albumen often wretched insufficiency, cause carp growth speed excessively slow, feed conversion rate is low, and feeding cycle is long, the grievous injury economic benefit of raiser; In grass carp breeding process, due under high-density breeding condition, obviously declined by the grass carp physique of feeding with fodder concentrate, and cultivation density cross conference produce stress, reduce fish to the resistance of disease, cause survival rate low, the infringement of the gill rot be easily subject in breeding process, red skin disease, hemorrhage and enteritis disease, these four kinds of diseases are called as " grass carp four is sick ", and the outburst of these diseases can bring destructive strike to grass carp aquaculture, and the cultivation of grass carp makes to be subject to very large restriction; Intensive culture mode is also day by day serious to the infringement of water environment in addition, thus causes aquatic products disease to take place frequently.Antibiotic use has certain effect to aquatic products disease prevention and cure tool within a certain period of time, but it is easy to expedite the emergence of out drug resistance strain, and Environmental Residues and the enrichment of fish body exist huge potential hazard to human health.Along with expanding economy, traditional aquatic products theory of raising no longer can meet the consumption demand that people are growing and improve, therefore, non-harmful feed addictive and non-harmful feed become the focus of feedstuff industry development in recent years, are also the new requirements that culturist and consumer propose fishery cultivating development.
Ginkgo is deciduous tree, and be Relict Plant the most ancient in existing seed plant, leaf is fan-shaped, scattered on long shoot, and brachyplast is clustered.Ginkgo is not only a kind of medicinal, economic fruit tree, in recent years along with the development of urban landscape construction and urban beautification, ginkgo has become a kind of ornamental plant, spread all over the country each avenue, but, except acting on above, the whole body of ginkgo is all precious, wherein ginkgo leaf is except having the nutrition of general leaf, it also containing multiple flavonoids, terpene lactones, polyprenols and alkaloid and polysaccharide isoreactivity composition, can be used as the raw materials for production of medicine, biological pesticide, food, cosmetics.Also there is research used as feed addictive composition simultaneously, Land use systems at present in feed industry mainly concentrates on and directly utilizes or add in feed after simple pulverizing, also there is part research that its alcohol extract is used as feed addictive, mainly play antioxidation, but its palatability is poor when directly utilizing, biologically active is not obvious, cellulose, the large molecule such as hemicellulose is difficult to by animal use, the mode of alcohol extracting is adopted to utilize, it only make use of the active component of wherein part alcohol dissolubility, give up the mass efficient such as cellulose and hemicellulose composition, utilization rate is not high yet.Therefore, the problem that ginkgo leaf utilizes effectively can not be solved in the market, at present also not by ginkgo leaf or its extract report for the production of feeding additive aquatic animal.
Summary of the invention
Utilize aspect Problems existing for ginkgo leaf in prior art, and culture fishery is for the demand of nuisance free feed supplement, it take ginkgo leaf as the nuisanceless feeding additive aquatic animal of base-material that the present invention aims to provide a kind of.
For solving above technical problem, the present invention adopts following technological means:
Take ginkgo leaf as a nuisanceless feeding additive aquatic animal for base-material, it is characterized in that, be made up of the raw material of following weight portion: ginkgo leaf 150-200 part, potato 100-120 part, lotus leaf 30-50 part, Herba Andrographitis 15-20 part, elscholtiza 8-12 part, rheum officinale 5-7 part, bark of official magnolia 7-12 part, fruit of negundo 3-5 part, chlorella powder 50-80 part, egg powder 50-80 part, medical stone 5-10 part, cornstarch 20-30 part.
Described is that the preparation method of the nuisanceless feeding additive aquatic animal of base-material comprises the steps: with ginkgo leaf
Step one: take each raw material by weight;
Step 2: by the potato cutting taken in step one, adds the water of its quality 5 times, and heating is boiled and kept boiling 35m I n, is then cooled to 40 DEG C; The ginkgo leaf chopping that simultaneously will take in step one, joins the potato cooled and boils and boil in liquid, and add a kind of medical stone taken of step, obtain mixture I;
Step 3: access the mixed bacteria be made up of aspergillus niger and aspergillus oryzae in mixture I, carries out aerobic fermentation when stirring, fermentation 3-5 days, then access Lactobacillus plantarum again, carry out standing anaerobic fermentation when sealing, fermentation 5-7 days, obtains mixture II;
Step 4: mixture II is filtered, obtain comprising thalline filtrate and filter residue, vacuum freeze drying is carried out to filtrate and obtains bacterium powder I, the mode of heated-air drying is adopted to carry out drying to filter residue, make its water content lower than 8%, then the filter residue of drying is pulverized, be crushed to 80-100 order, obtain mixture III;
Step 5: be crushed to 80-100 order after the lotus leaf taken in step one, Herba Andrographitis, elscholtiza, rheum officinale, the bark of official magnolia, fruit of negundo, chlorella powder, egg powder and cornstarch being mixed, obtain mixture IV;
Step 6: by the bacterium powder I obtained in step 4
,mixture IV in mixture III and step 5 mixes, and mixes that namely to obtain described take ginkgo leaf as the nuisanceless feeding additive aquatic animal of base-material thoroughly.
Preferably, be made up of the raw material of following weight portion: ginkgo leaf 180 parts, potato 110 parts, 40 parts, lotus leaf, Herba Andrographitis 17 parts, elscholtiza 9 parts, rheum officinale 6 parts, the bark of official magnolia 9 parts, fruit of negundo 4 parts, chlorella powder 60 parts, egg powder 60 parts, medical stone 7 parts, cornstarch 25 parts.
The raising taking ginkgo leaf as the nuisanceless feeding additive aquatic animal of base-material and can be widely used in freshwater fish of the present invention, preferably may be used for the raising of grass carp, carp and black carp, and its addition in feed is 1.0-2.0%.
Of the present invention is that the nuisanceless feeding additive aquatic animal of base-material has the following advantages and beneficial effect with ginkgo leaf:
First, the present invention adopts urban waste-ginkgo leaf to be base-material, by the mode of two-step fermentation, fermentation process is carried out to ginkgo leaf, the wherein combination of aspergillus niger and aspergillus oryzae, can beta-glucosidase be produced, the flavour of a drug of ginkgo leaf and blue or green gas can be made all to weaken or disappear by its biological conversion, and the glucoside type flavone compound of ginkgo leaf can be made to be converted into flavonols, enhance biologically active, reach the effect of debitterize; Simultaneously aspergillus niger and aspergillus oryzae can be degraded materials such as utilizing cellulose in ginkgo leaf and hemicellulose, be degraded to the utilizable Small molecular carbohydrate of Lactobacillus plantarum, therefore after aspergillus niger and aspergillus oryzae fermentation, Lactobacillus plantarum fermentation is accessed again, both the Lactobacillus plantarum bacterium liquid of higher concentration can have been obtained, simultaneously by above zymotechnique, the fragrance of flowers and fruits and the mouthfeel of sweet acid can be produced, improve smell and the mouthfeel of ginkgo leaf.
Secondly, do not add any harmful adding ingredient in the preparation process in accordance with the present invention, in initial fermentation, select potato as carbon source, wherein be rich in the carbohydrates such as starch, contribute to the quick growth at the fermentation initial stage of aspergillus niger and aspergillus oryzae, in fermentation system, add medical stone, trace element wherein and macroelement discharge slowly in the process of fermentation, can ensure the nutrient demand in fermentation process; And both belong to natural food or traditional Chinese medicinal material raw materials, toxic action can not be produced to animal.
Again, multiple selected Chinese medicine composition is with the addition of in nuisanceless feeding additive aquatic animal of the present invention, cooperatively interact between itself and ginkgo leaf, to aquatic livestock grow and the raising of immunity has good facilitation, the effect of diseases prevention can also be played simultaneously.Wherein rheum officinale, the bark of official magnolia, Herba Andrographitis and fruit of negundo with the use of, can be good at playing antibacterial disease-resistant effect; The cooperation of egg powder and chlorella powder, makes feed have certain fishy smell, has good food calling effect to fish, is wherein rich in multiple proteins and vitamin simultaneously, can meets the nutritional need of fish, contributes to aquatic livestock and improves immunity.
Of the present invention is that the nuisanceless feeding additive aquatic animal of base-material adopts specific technique to process ginkgo leaf with ginkgo leaf, again in conjunction with plurality of Chinese composition, the object improving aquatic livestock immunity, promote aquatic animal to grow can be reached well, and feed addictive of the present invention all adopts natural component, without any harmful chemical agents extraction process, pollution-free to culture environment of aquatic products, meet modern aquaculture industry green, safety, non-harmful requirement completely.
Specific embodiment
embodiment 1: a kind of take ginkgo leaf as the nuisanceless feeding additive aquatic animal of base-material, is made up: ginkgo leaf 180 parts, potato 110 parts of the raw material of following weight portion, 40 parts, lotus leaf, Herba Andrographitis 17 parts, elscholtiza 9 parts, rheum officinale 6 parts, the bark of official magnolia 9 parts, fruit of negundo 4 parts, chlorella powder 60 parts, egg powder 60 parts, medical stone 7 parts, cornstarch 25 parts.
Described is that the preparation method of the nuisanceless feeding additive aquatic animal of base-material comprises the steps: with ginkgo leaf
Step one: take each raw material by weight;
Step 2: by the potato cutting taken in step one, adds the water of its quality 5 times, and heating is boiled and kept boiling 35m I n, is then cooled to 40 DEG C; The ginkgo leaf chopping that simultaneously will take in step one, joins the potato cooled and boils and boil in liquid, and add a kind of medical stone taken of step, obtain mixture I;
Step 3: access the mixed bacteria be made up of aspergillus niger and aspergillus oryzae in mixture I, carries out aerobic fermentation when stirring, fermentation 3-5 days, then access Lactobacillus plantarum again, carry out standing anaerobic fermentation when sealing, fermentation 5-7 days, obtains mixture II;
Step 4: mixture II is filtered, obtain comprising thalline filtrate and filter residue, vacuum freeze drying is carried out to filtrate and obtains bacterium powder I, the mode of heated-air drying is adopted to carry out drying to filter residue, make its water content lower than 8%, then the filter residue of drying is pulverized, be crushed to 80-100 order, obtain mixture III;
Step 5: be crushed to 80-100 order after the lotus leaf taken in step one, Herba Andrographitis, elscholtiza, rheum officinale, the bark of official magnolia, fruit of negundo, chlorella powder, egg powder and cornstarch being mixed, obtain mixture IV;
Step 6: by the bacterium powder I obtained in step 4
,mixture IV in mixture III and step 5 mixes, and mixes that namely to obtain described take ginkgo leaf as the nuisanceless feeding additive aquatic animal of base-material thoroughly.
embodiment 2: a kind of take ginkgo leaf as the nuisanceless feeding additive aquatic animal of base-material, is made up: ginkgo leaf 150 parts, potato 120 parts of the raw material of following weight portion, 35 parts, lotus leaf, Herba Andrographitis 20 parts, elscholtiza 10 parts, rheum officinale 6 parts, the bark of official magnolia 10 parts, fruit of negundo 5 parts, chlorella powder 80 parts, egg powder 50 parts, medical stone 8 parts, cornstarch 25 parts.
Described is that the preparation method of the nuisanceless feeding additive aquatic animal of base-material comprises the steps: with ginkgo leaf
Step one: take each raw material by weight;
Step 2: by the potato cutting taken in step one, adds the water of its quality 5 times, and heating is boiled and kept boiling 35m I n, is then cooled to 40 DEG C; The ginkgo leaf chopping that simultaneously will take in step one, joins the potato cooled and boils and boil in liquid, and add a kind of medical stone taken of step, obtain mixture I;
Step 3: access the mixed bacteria be made up of aspergillus niger and aspergillus oryzae in mixture I, carries out aerobic fermentation when stirring, fermentation 3-5 days, then access Lactobacillus plantarum again, carry out standing anaerobic fermentation when sealing, fermentation 5-7 days, obtains mixture II;
Step 4: mixture II is filtered, obtain comprising thalline filtrate and filter residue, vacuum freeze drying is carried out to filtrate and obtains bacterium powder I, the mode of heated-air drying is adopted to carry out drying to filter residue, make its water content lower than 8%, then the filter residue of drying is pulverized, be crushed to 80-100 order, obtain mixture III;
Step 5: be crushed to 80-100 order after the lotus leaf taken in step one, Herba Andrographitis, elscholtiza, rheum officinale, the bark of official magnolia, fruit of negundo, chlorella powder, egg powder and cornstarch being mixed, obtain mixture IV;
Step 6: by the bacterium powder I obtained in step 4
,mixture IV in mixture III and step 5 mixes, and mixes that namely to obtain described take ginkgo leaf as the nuisanceless feeding additive aquatic animal of base-material thoroughly.
embodiment 3: safety testing
Acute toxicity test in mice: mix 15%(weight percentage in the feed of small white mouse) the feed addictive of the embodiment of the present invention 1, feed after this mix is used for feeding of small white mouse, Continuous Observation 20 days, duration of test has no dead and undue toxicity reaction, off-test, Dissection test mouse, observes liver,kidney,spleen, stomach, the heart, lung and intestines, is showed no abnormal change.By standard of acute toxicity, this feed addictive belongs to nontoxic level.
Chronic toxicity test: mix 7%(weight percentage in the feed of small white mouse) the feed addictive of the embodiment of the present invention 1, feed after this mix is used for feeding of small white mouse, feed continuously 120 days, result shows: the mouse fur of the feed comprising the embodiment of the present invention 1 feed addictive of feeding is smooth, behavioral activity is normal, drinking-water of ingesting is normal, Normal-weight increases, blood parameters and hematology all fluctuate in normal range (NR), routine urinalysis and marrow index all fluctuate in normal range (NR), organize internal organs no abnormality seen, generally speaking toxicity reaction is had no.
From testing above, product of the present invention is safe, nontoxic.
embodiment 4: Animal experiment
Comparative experimental example 1 and 2 is below set:
comparative experimental example 1: a kind of feed addictive, be made up of the raw material of following weight portion: ginkgo leaf 180 parts, potato 110 parts, 40 parts, lotus leaf, the bark of official magnolia 9 parts, fruit of negundo 4 parts, chlorella powder 60 parts, egg powder 60 parts, medical stone 7 parts, cornstarch 25 parts.The preparation method of described feed addictive comprises the steps:
Step one: take each raw material by weight;
Step 2: by the potato cutting taken in step one, adds the water of its quality 5 times, and heating is boiled and kept boiling 35m I n, is then cooled to 40 DEG C; The ginkgo leaf chopping that simultaneously will take in step one, joins the potato cooled and boils and boil in liquid, and add a kind of medical stone taken of step, obtain mixture I;
Step 3: access the mixed bacteria be made up of aspergillus niger and aspergillus oryzae in mixture I, carries out aerobic fermentation when stirring, fermentation 3-5 days, then access Lactobacillus plantarum again, carry out standing anaerobic fermentation when sealing, fermentation 5-7 days, obtains mixture II;
Step 4: mixture II is filtered, obtain comprising thalline filtrate and filter residue, vacuum freeze drying is carried out to filtrate and obtains bacterium powder I, the mode of heated-air drying is adopted to carry out drying to filter residue, make its water content lower than 8%, then the filter residue of drying is pulverized, be crushed to 80-100 order, obtain mixture III;
Step 5: be crushed to 80-100 order after the lotus leaf taken in step one, the bark of official magnolia, fruit of negundo, chlorella powder, egg powder and cornstarch being mixed, obtain mixture IV;
Step 6: by the bacterium powder I obtained in step 4
,mixture IV in mixture III and step 5 mixes, and mixes the feed addictive namely obtaining comparative experimental example 1 thoroughly.
comparative experimental example 2: a kind of feed addictive, be made up of the raw material of following weight portion: ginkgo leaf 180 parts, potato 110 parts, 40 parts, lotus leaf, Herba Andrographitis 17 parts, elscholtiza 9 parts, the bark of official magnolia 9 parts, fruit of negundo 4 parts, medical stone 7 parts, cornstarch 25 parts.The preparation method of described feed addictive comprises the steps:
Step one: take each raw material by weight;
Step 2: by the potato cutting taken in step one, adds the water of its quality 5 times, and heating is boiled and kept boiling 35m I n, is then cooled to 40 DEG C; The ginkgo leaf chopping that simultaneously will take in step one, joins the potato cooled and boils and boil in liquid, and add a kind of medical stone taken of step, obtain mixture I;
Step 3: access the mixed bacteria be made up of aspergillus niger and aspergillus oryzae in mixture I, carries out aerobic fermentation when stirring, and fermentation 3-5 days, obtains mixture II;
Step 4: mixture II is filtered, obtain comprising thalline filtrate and filter residue, vacuum freeze drying is carried out to filtrate and obtains bacterium powder I, the mode of heated-air drying is adopted to carry out drying to filter residue, make its water content lower than 8%, then the filter residue of drying is pulverized, be crushed to 80-100 order, obtain mixture III;
Step 5: be crushed to 80-100 order after the lotus leaf taken in step one, Herba Andrographitis, elscholtiza, the bark of official magnolia, fruit of negundo and cornstarch being mixed, obtain mixture IV;
Step 6: by the bacterium powder I obtained in step 4
,mixture IV in mixture III and step 5 mixes, and mixes the feed addictive namely obtaining comparative experimental example 2 thoroughly.
(1) grass carp feeding experiment
Get same a slice waters, same batch of grass carp aquaculture net cage of putting in a suitable place to breed 5, cultivation grass carp 200 tail in every net cage, be divided into 5 groups at random, group 1 is blank group, the grass carp pellet of throwing something and feeding commercially available; Group 2-3 is test group, and its feed of throwing something and feeding is the grass carp pellet that the feeding additive aquatic animal of the embodiment of the present invention 1,2 that with the addition of 1.5% on the basis of commercially available grass carp pellet formula also still conventionally processes; Group 4-5 is check experiment group, and its feed of throwing something and feeding is the grass carp pellet that the feed addictive of the comparative experimental example 1,2 that with the addition of 1.5% on the basis of commercially available grass carp pellet formula also still conventionally processes.Feed and gather in the crops (i.e. whole test duration 60 days) afterwards in 60 days, 5 tails are fished at random for each group before feeding, take its initial weight and on tail fin, stay corresponding label, feeding experiment terminates the rear body weight continuing fish that take survival, that mark, represent the average weight growth pattern of each group of grass carp, record the death rate of each group of grass carp in the process of feeding, concrete outcome is as follows simultaneously:
Group 1: blank group, average growth rate 219.3%, dead grass carp 15 tail of this net cage of period, the death rate is 7.5%, and death does not occur mark grass carp.The individual entirety of this group grass carp is less.
Group 2: embodiment 1 group, average growth rate 327.9%, period, this net cage was dead for there is grass carp, and namely the death rate is 0%.This group grass carp individuality is maximum in 5 groups, and counterpoise reaches 4.76kg.
Group 3: embodiment 2 groups, average growth rate 312.2%, dead grass carp 1 tail of this net cage of period, the death rate is 0.5%, and death does not occur mark grass carp.This group grass carp individuality is secondary large in 5 groups, and counterpoise reaches 4.57kg.
Group 4: comparative example 1 group, average growth rate 261.3%, dead grass carp 5 tail of this net cage of period, the death rate is 2.5%, and death does not occur mark grass carp.This group grass carp is individual little.
Group 5: comparative example 2 groups, average growth rate 258.1%, dead grass carp 9 tail of this net cage of period, the death rate is 4.5%, and death does not occur mark grass carp.This group grass carp individuality is less than normal.
Wherein average growth rate=(the initial population weight of test TBW in the latter stage-mark fish of mark fish)/initial population heavy * 100%.
From above result of the test, product of the present invention can promote the growth of grass carp effectively, its average growth rate is apparently higher than blank group and group 4(comparative experimental example 1), group 5(comparative experimental example 2), no matter illustrate it is formula composition or preparation technology, reaching of technique effect is all absolutely necessary.Adopt product of the present invention to throw something and feed, the death rate of grass carp comparatively organizes 1, group 4-5 also declines to some extent, prove that feed addictive of the present invention can improve the immunity of grass carp effectively, improve its resistance against diseases.
(2) carp feeding experiment
Get 5, same a slice waters, same batch of carp culture net cage of putting in a suitable place to breed, cultivation carp 100 tail in every net cage, be divided into 5 groups at random, group 1 is blank group, the carp pellet of throwing something and feeding commercially available; Group 2-3 is test group, and its feed of throwing something and feeding is the carp pellet that the feeding additive aquatic animal of the embodiment of the present invention 1,2 that with the addition of 2.0% on the basis of commercially available carp pellet formula also still conventionally processes; Group 4-5 is check experiment group, and its feed of throwing something and feeding is the carp pellet that the feed addictive of the comparative experimental example 1,2 that with the addition of 2.0% on the basis of commercially available carp pellet formula also still conventionally processes.Feed and gather in the crops (i.e. whole test duration 80 days) afterwards in 80 days, 5 tails are fished at random for each group before feeding, take its initial weight and on tail fin, stay corresponding label, feeding experiment terminates the rear body weight continuing fish that take survival, that mark, represent the average weight growth pattern of each group of carp, record the death rate of each group of carp in the process of feeding, concrete outcome is as follows simultaneously:
Group 1: blank group, average growth rate 199.4%, dead carp 8 tail of this net cage of period, the death rate is 8.0%, and death does not occur mark carp.This group carp individual entirety is less.
Group 2: embodiment 1 group, average growth rate 279.3%, dead carp 0 tail of this net cage of period, namely the death rate is 0%.This group carp individual is maximum in 5 groups, and counterpoise reaches 3.27kg.
Group 3: embodiment 2 groups, average growth rate 267.4%, dead carp 2 tail of this net cage of period, the death rate is 2.0%, and death does not occur mark carp.This group carp individual is secondary large in 5 groups, and counterpoise reaches 3.07kg.
Group 4: comparative example 1 group, average growth rate 212.2%, dead carp 4 tail of this net cage of period, the death rate is 4.0%, and death does not occur mark carp.This group carp individual is little.
Group 5: comparative example 2 groups, average growth rate 218.7%, dead carp 9 tail of this net cage of period, the death rate is 9.0%, and death does not occur mark carp.This group carp individual is less than normal.
Wherein average growth rate=(the initial population weight of test TBW in the latter stage-mark fish of mark fish)/initial population heavy * 100%.
From above result of the test, product of the present invention can promote the growth of carp effectively, its average growth rate is apparently higher than blank group and group 4(comparative experimental example 1), group 5(comparative experimental example 2), no matter illustrate it is formula composition or preparation technology, reaching of technique effect is all absolutely necessary.Adopt product of the present invention to throw something and feed, the death rate of carp comparatively organizes 1, group 4-5 also declines to some extent, prove that feed addictive of the present invention can improve the immunity of carp effectively, improve its resistance against diseases.
In the extension process of product of the present invention, find that it has good facilitation to the growth of Freshwater shrimps, crucian, black carp.
Raw material of the present invention all can be commercially.Be only specific embodiments of the invention above, but the present invention is not limited thereto, the changes that any person skilled in the art can think of, all should drops within protection scope of the present invention.
Claims (3)
1. one kind take ginkgo leaf as the nuisanceless feeding additive aquatic animal of base-material, it is characterized in that, be made up of the raw material of following weight portion: ginkgo leaf 150-200 part, potato 100-120 part, lotus leaf 30-50 part, Herba Andrographitis 15-20 part, elscholtiza 8-12 part, rheum officinale 5-7 part, bark of official magnolia 7-12 part, fruit of negundo 3-5 part, chlorella powder 50-80 part, egg powder 50-80 part, medical stone 5-10 part, cornstarch 20-30 part, described is that the preparation method of the nuisanceless feeding additive aquatic animal of base-material comprises the steps: with ginkgo leaf
Step one: take each raw material by weight;
Step 2: by the potato cutting taken in step one, adds the water of its quality 5 times, and heating is boiled and kept the 35min that seethes with excitement, and is then cooled to 40 DEG C; The ginkgo leaf chopping that simultaneously will take in step one, joins the potato cooled and boils and boil in liquid, and add the medical stone taken in step one, obtain mixture I;
Step 3: access the mixed bacteria be made up of aspergillus niger and aspergillus oryzae in mixture I, carries out aerobic fermentation when stirring, fermentation 3-5 days, then access Lactobacillus plantarum again, carry out standing anaerobic fermentation when sealing, fermentation 5-7 days, obtains mixtures II;
Step 4: mixtures II is filtered, obtain comprising thalline filtrate and filter residue, vacuum freeze drying is carried out to filtrate and obtains bacterium powder I, the mode of heated-air drying is adopted to carry out drying to filter residue, make its water content lower than 8%, then the filter residue of drying is pulverized, be crushed to 80-100 order, obtain mixtures III;
Step 5: be crushed to 80-100 order after the lotus leaf taken in step one, Herba Andrographitis, elscholtiza, rheum officinale, the bark of official magnolia, fruit of negundo, chlorella powder, egg powder and cornstarch being mixed, obtain mixture IV;
Step 6: mixed by the mixture IV in the bacterium powder I, mixtures III and the step 5 that obtain in step 4, mixes that namely to obtain described take ginkgo leaf as the nuisanceless feeding additive aquatic animal of base-material thoroughly.
2. according to claim 1 take ginkgo leaf as the nuisanceless feeding additive aquatic animal of base-material, it is characterized in that, is made up of the raw material of following weight portion: ginkgo leaf 180 parts, potato 110 parts, 40 parts, lotus leaf, Herba Andrographitis 17 parts, elscholtiza 9 parts, rheum officinale 6 parts, the bark of official magnolia 9 parts, fruit of negundo 4 parts, chlorella powder 60 parts, egg powder 60 parts, medical stone 7 parts, cornstarch 25 parts.
3. preparing described in claim 1 or 2 take ginkgo leaf as the method for the nuisanceless feeding additive aquatic animal of base-material, it is characterized in that, comprises the steps:
Step one: take each raw material by weight;
Step 2: by the potato cutting taken in step one, adds the water of its quality 5 times, and heating is boiled and kept the 35min that seethes with excitement, and is then cooled to 40 DEG C; The ginkgo leaf chopping that simultaneously will take in step one, joins the potato cooled and boils and boil in liquid, and add the medical stone taken in step one, obtain mixture I;
Step 3: access the mixed bacteria be made up of aspergillus niger and aspergillus oryzae in mixture I, carries out aerobic fermentation when stirring, fermentation 3-5 days, then access Lactobacillus plantarum again, carry out standing anaerobic fermentation when sealing, fermentation 5-7 days, obtains mixtures II;
Step 4: mixtures II is filtered, obtain comprising thalline filtrate and filter residue, vacuum freeze drying is carried out to filtrate and obtains bacterium powder I, the mode of heated-air drying is adopted to carry out drying to filter residue, make its water content lower than 8%, then the filter residue of drying is pulverized, be crushed to 80-100 order, obtain mixtures III;
Step 5: be crushed to 80-100 order after the lotus leaf taken in step one, Herba Andrographitis, elscholtiza, rheum officinale, the bark of official magnolia, fruit of negundo, chlorella powder, egg powder and cornstarch being mixed, obtain mixture IV;
Step 6: mixed by the mixture IV in the bacterium powder I, mixtures III and the step 5 that obtain in step 4, mixes that namely to obtain described take ginkgo leaf as the nuisanceless feeding additive aquatic animal of base-material thoroughly.
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| CN106417127A (en) * | 2016-10-13 | 2017-02-22 | 合肥润雨农业科技有限公司 | Handling method for lowering content of diethylstilbestrol in prawns |
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| CN1380098A (en) * | 2002-05-20 | 2002-11-20 | 张勇飞 | Anti-infection compound preparation and its preparation method |
| CN101543259A (en) * | 2009-05-08 | 2009-09-30 | 南京林业大学 | Preparation method of biological feed additive |
| CN101708030A (en) * | 2008-09-07 | 2010-05-19 | 王妍 | Ginkgo biloba leaf feed |
| CN102283330A (en) * | 2011-04-02 | 2011-12-21 | 大连理工大学 | Sea cucumber accelerating feed and preparation method thereof |
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|---|---|---|---|---|
| CN1380098A (en) * | 2002-05-20 | 2002-11-20 | 张勇飞 | Anti-infection compound preparation and its preparation method |
| CN101708030A (en) * | 2008-09-07 | 2010-05-19 | 王妍 | Ginkgo biloba leaf feed |
| CN101543259A (en) * | 2009-05-08 | 2009-09-30 | 南京林业大学 | Preparation method of biological feed additive |
| CN102283330A (en) * | 2011-04-02 | 2011-12-21 | 大连理工大学 | Sea cucumber accelerating feed and preparation method thereof |
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