CN105558434A - Composition with fish luring function - Google Patents
Composition with fish luring function Download PDFInfo
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- CN105558434A CN105558434A CN201610040042.7A CN201610040042A CN105558434A CN 105558434 A CN105558434 A CN 105558434A CN 201610040042 A CN201610040042 A CN 201610040042A CN 105558434 A CN105558434 A CN 105558434A
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Abstract
The invention relates to a novel natural composition remarkable in fish luring effect. The composition is prepared from, by weight, 0.5-1 part of silver carp gill extracts, 1-3 parts of herba houttuyniae extracts and 0.1-0.3 part of betel leaf oil. The composition has a remarkable carp luring function and is natural and environmentally friendly.
Description
Technical field
The present invention relates to cultivation field, especially aquaculture field.
Background technology
Aquatic animal feed phagostimulant is a kind of non-nutritive additive, and it is the material of chemical composition Prof. Du Yucang contained by the animals and plants of the most eating of aquatic livestock or extraction.Phagostimulant is often made up of two or more compounds, and these compounds, to the synergy of having ingested of aquatic livestock, are produced by the sense of smell to aquatic livestock, the sense of taste and vision etc. and stimulate, make it gather around feed, accelerate to ingest raising feed intake.In bait, add phagostimulant to throw something and feed cultured fishes, can effectively promote ingesting of fish, improve the feed intake of fish, reduce surplus bait in water, alleviate the pollution of residual bait corruption to water quality.
China's fish production is mainly based on intensive culture, but the utilization rate of fish feed is relatively low, cause the higher (average out to 1.93 of feed coefficient, be up to 2.36), fish feed does not obtain the utilization that should use up, cause serious waste to strengthen fish culture cost, pollute breeding water body again simultaneously.
So the exploitation of novel phagostimulant has important practical significance.
Silver carp (Hypophthalmichthysmolitrix), cries again silver carp, water silver carp, jumps silver carp, Hypophthalmichthys molitrix, belong to Cypriniformes, Cyprinidae, is one of famous four large Chinese carps.
The bodily form is flat-sided, slightly high, in spindle, and back cinerous, both sides and belly white.Pectoral fin is no more than abdomeinal fin base portion.Each fin look greyish white.Head is larger.Eye position is very low.Scale is tiny.Belly center cutin rib directly prolongs and reaches anus below pectoral fin.Form is similar with bighead, and silver carp is impatient hot-tempered, kind jump.
Silver carp taste is sweet, and property is put down, and nontoxic, it is Fresh & Tender in Texture, nutritious, is one of excellent fingerling that comparatively should cultivate.For one of cultured freshwater fish that China is main.Be distributed in each large water system in the whole nation.Silver carp is the large-scale fresh-water fishes of artificial feeding, and growth is fast, disease is few, output is high, and many and grass carp, carp are raised together with.
In prior art, the fish gill is usually by as castoff of food and drink process.
Cordate houttuynia (English name HERBAHOUTTUYNIAE) is the herbal medicine that Chinese Pharmacopoeia is included, and herbal medicine source is the dry aerial parts of saururaceae plant houttuynia cordata (Classification system: HouttuyniacordataThunb.).Cordate houttuynia taste is pungent, cold, returns lung channel.Clearing heat and detoxicating, detumescence can treat sore, promoting urination and removing dampness, clearing away heat to cure dysentery, stomach strengthening and digestion promoting, by the lung carbuncle, sore swollen toxin, hemorrhoidal hemorrhage, retention of heat in the spleen and stomach etc. of controlling real heat, heat poison, damp evil, disease heat are trouble.Modern pharmacology experiment shows, this product has antibacterial, antiviral, effect such as raising immunity of organisms, diuresis etc.
Beach wormwood oil, for the leaf of Piperaceae Zhi Wu Betel sauce PiperbetleL. is through distillable aromatic oil.Control brothers' redness or pain, rub with oil betel, wrap up with cloth.Drip ear and control otalgia.Knife wound is hindered, and pastes wrap up in injury with cotton leaching beach wormwood oil.Control again carbuncle on the back and furuncle poison, paste it, namely dismiss from the beginning of person, become the routed purulence of i.e. speed.Also can apply ointment or plaster red bayberry carbuncle, leak hemorrhoid.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of natural attractant of Be very effective.
In order to solve the problems of the technologies described above, the present invention proposes following technical proposal:
There is a composition for attracting effect, it is characterized in that described composition is made up of the material of following weight portion: silver carp gill extract 0.5-1 part, houttuynia extract 1-3 part, beach wormwood oil 0.1-0.3 part;
Described silver carp gill extract is prepared from by following method: the gill getting fresh silver carp, add the physiological saline of its dry weight 8 times of quality, high speed shear homogenate, by watery hydrochloric acid adjust ph to 2-3, add pepsin, the quality that described pepsin adds is 2% of gill dry weight, described pepsic vigor is 4000-5000U/g, enzymolysis 12 hours under 37 DEG C of conditions, add papain, the quality that described papain adds is 0.2% of gill dry weight, the vigor of described papain is 300,000-50 ten thousand U/g, enzymolysis is continued 5 hours under 37 DEG C of conditions, centrifugal, get supernatant liquid, add ethanol, make the volume percent content to 30% of ethanol in solution, leave standstill 12 hours, separation of supernatant, ultrafiltration, retain the material that relative molecular weight is 5000-20000, freeze drying, obtain.
Described houttuynia extract has following method to be prepared from: get fresh cordate houttuynia, adds the ratio of 10g water, add water in every 1g cordate houttuynia, decocts 3 times, each 1.5 hours, merges decoction liquor, filters, dry, to obtain final product.
Preferably, described composition is made up of the material of following weight portion: silver carp gill extract 0.8 part, houttuynia extract 2 parts, beach wormwood oil 0.2 part.
Beneficial effect of the present invention can be proved by following test.
Test example 1 phagostimulant of the present invention is to the phagostimulating effect of carp
Material and reagent
For reagent thing 1: the present composition, prepare by embodiment 9, during test, use water is mixed with the concentration of 20mg/L.
During test, what measure that 10mL prepares with graduated cylinder pours in the small beaker of 50mL for reagent thing, and the medical absorbent cotton ball that skin radius being about 1.5cm is surrounded by gauze is put into and wherein soaked 2min, and control group is tried and replaced reagent to do same process with distilled water.
Test fish: squama carp, its specification is average body long (12 ± 1) cm, average weight (121 ± 5) g.
Test method
Bian is with stinging ball: the test fish newly the purchased disinfecting solution of potassium permanganate 10 ~ 15min of 8%, and support temporarily in the aquarium that circulating water device is housed, pellet of throwing something and feeding, ensures that fish is satiated with food, and observes fish normal activity 7d, is selected by unsound fish.By types of agents, aquarium is set, 15 tail squama carps put into by each aquarium, and the reagent ball of well in advance is hanging in aquarium, reagent ball be positioned at aquarium middle and at water 15cm place, dropping into tries to start to record continuously every 5min squama carp after 3min and to peck at the total degree of trying, and records 20min altogether.Separately establish one group as a control group, once, cleaning aquarium after each experiment also changes water, and experimental session water temperature is (19 ± 1) DEG C, and pH is 6.5-7.3 in squama carp experiment every day of each aquarium.Keep control group all consistent with each experiment condition of experimental group.Each confession reagent does three parallel group.
The data of each test represent result of the test with the mean+/-standard error of each repeated observation value, analyze data with SPSS13.0 statistical package, t inspection between employing group.
Result of the test
Result of the test is in table 1, and result shows, the present composition has significant food calling effect, and compared with blank group, the difference of number of times of pecking at has statistical significance (P<0.01).
The phagostimulating effect of table 1 phagostimulant of the present invention
Group | On average sting ball number of times |
Blank group | 15.25±7.63 |
For reagent thing 1 | 55.27±10.57** |
Note: compared with control group, * * P<0.01.
In order to better set forth technical scheme of the present invention, below in conjunction with detailed description of the invention, the present invention is further illustrated, but protection domain of the presently claimed invention is not limited to the following example.
Detailed description of the invention
The preparation of embodiment 1 silver carp gill extract
Get the gill 50Kg of fresh silver carp, add the physiological saline of its dry weight 8 times of quality, high speed shear homogenate, by watery hydrochloric acid adjust ph to 2-3, add pepsin, the quality that described pepsin adds is 2% of gill dry weight, described pepsic vigor is 4000-5000U/g, enzymolysis 12 hours under 37 DEG C of conditions, add papain, the quality that described papain adds is 0.2% of gill dry weight, the vigor of described papain is 300,000-50 ten thousand U/g, enzymolysis is continued 5 hours under 37 DEG C of conditions, centrifugal, get supernatant liquid, add ethanol, make the volume percent content to 30% of ethanol in solution, leave standstill 12 hours, separation of supernatant, ultrafiltration, retain the material that relative molecular weight is 5000-20000, freeze drying, obtain phagostimulant 3.5g of the present invention.
The preparation of embodiment 2 houttuynia extract
Get fresh cordate houttuynia 1Kg, add the ratio of 10g water in every 1g cordate houttuynia, add water, decoct 3 times, each 1.5 hours, merge decoction liquor, filter, dry, obtain 213g.
The preparation of embodiment 3 attracting composition
Get silver carp gill extract (by the preparation of embodiment 1 method) 0.5g, houttuynia extract (by the preparation of embodiment 2 method) 3g, mixing, evenly spray into beach wormwood oil 0.3g, sealing, to obtain final product.
Method with reference to test example 1 carries out food calling test, and result shows (the results are shown in Table 2), and the present embodiment phagostimulant has obvious phagostimulating effect.
The phagostimulating effect of table 2 phagostimulant of the present invention
Group | On average sting ball number of times |
Blank group | 15.25±7.63 |
The present embodiment phagostimulant | 52.20±14.38** |
Note: compared with control group, * * P<0.01.
The preparation of embodiment 4 attracting composition
Get silver carp gill extract (by the preparation of embodiment 1 method) 1g, houttuynia extract (by the preparation of embodiment 2 method) 1g, mixing, evenly spray into beach wormwood oil 0.1g, sealing, to obtain final product.
Method with reference to test example 1 carries out food calling test, and result shows (the results are shown in Table 3), and the present embodiment phagostimulant has obvious phagostimulating effect.
The phagostimulating effect of table 3 phagostimulant of the present invention
Group | On average sting ball number of times |
Blank group | 15.25±7.63 |
The present embodiment phagostimulant | 52.19±13.97** |
Note: compared with control group, * * P<0.01.
The preparation of embodiment 5 attracting composition
Get silver carp gill extract (by the preparation of embodiment 1 method) 1g, houttuynia extract (by the preparation of embodiment 2 method) 1g, mixing, evenly spray into beach wormwood oil 0.3g, sealing, to obtain final product.
Method with reference to test example 1 carries out food calling test, and result shows (the results are shown in Table 4), and the present embodiment phagostimulant has obvious phagostimulating effect.
The phagostimulating effect of table 4 phagostimulant of the present invention
Group | On average sting ball number of times 3--> |
Blank group | 15.25±7.63 |
The present embodiment phagostimulant | 50.77±10.25** |
Note: compared with control group, * * P<0.01.
The preparation of embodiment 6 attracting composition
Get silver carp gill extract (by the preparation of embodiment 1 method) 0.5g, houttuynia extract (by the preparation of embodiment 2 method) 3g, mixing, evenly spray into beach wormwood oil 0.1g, sealing, to obtain final product.
Method with reference to test example 1 carries out food calling test, and result shows (the results are shown in Table 5), and the present embodiment phagostimulant has obvious phagostimulating effect.
The phagostimulating effect of table 5 phagostimulant of the present invention
Group | On average sting ball number of times |
Blank group | 15.25±7.63 |
The present embodiment phagostimulant | 49.86±15.73** |
Note: compared with control group, * * P<0.01.
The preparation of embodiment 7 attracting composition
Get silver carp gill extract (by the preparation of embodiment 1 method) 0.5g, houttuynia extract (by the preparation of embodiment 2 method) 1g, mixing, evenly spray into beach wormwood oil 0.3g, sealing, to obtain final product.
Method with reference to test example 1 carries out food calling test, and result shows (the results are shown in Table 6), and the present embodiment phagostimulant has obvious phagostimulating effect.
The phagostimulating effect of table 6 phagostimulant of the present invention
Group | On average sting ball number of times |
Blank group | 15.25±7.63 |
The present embodiment phagostimulant | 42.38±12.67** |
Note: compared with control group, * * P<0.01.
The preparation of embodiment 8 attracting composition
Get silver carp gill extract (by the preparation of embodiment 1 method) 1g, houttuynia extract (by the preparation of embodiment 2 method) 3g, mixing, evenly spray into beach wormwood oil 0.1g, sealing, to obtain final product.
Method with reference to test example 1 carries out food calling test, and result shows (the results are shown in Table 7), and the present embodiment phagostimulant has obvious phagostimulating effect.
The phagostimulating effect of table 7 phagostimulant of the present invention
Group | On average sting ball number of times |
Blank group | 15.25±7.63 |
The present embodiment phagostimulant | 44.51±16.29** |
Note: compared with control group, * * P<0.01.
The preparation of embodiment 9 attracting composition
Get silver carp gill extract (by the preparation of embodiment 1 method) 0.8g, houttuynia extract (by the preparation of embodiment 2 method) 2g, mixing, evenly spray into beach wormwood oil 0.2g, sealing, to obtain final product.
Test effect is shown in test example 1.
Claims (4)
1. there is a composition for attracting effect, it is characterized in that described composition is made up of the material of following weight portion: silver carp gill extract 0.5-1 part, houttuynia extract 1-3 part, beach wormwood oil 0.1-0.3 part.
2. a kind of composition with attracting effect according to claim 1, it is characterized in that described silver carp gill extract is prepared from by following method: the gill getting fresh silver carp, add the physiological saline of its dry weight 8 times of quality, high speed shear homogenate, by watery hydrochloric acid adjust ph to 2-3, add pepsin, the quality that described pepsin adds is 2% of gill dry weight, described pepsic vigor is 4000-5000U/g, enzymolysis 12 hours under 37 DEG C of conditions, add papain, the quality that described papain adds is 0.2% of gill dry weight, the vigor of described papain is 300,000-50 ten thousand U/g, enzymolysis is continued 5 hours under 37 DEG C of conditions, centrifugal, get supernatant liquid, add ethanol, make the volume percent content to 30% of ethanol in solution, leave standstill 12 hours, separation of supernatant, ultrafiltration, retain the material that relative molecular weight is 5000-20000, freeze drying, obtain.
3. a kind of composition with attracting effect according to claim 1,2, it is characterized in that described houttuynia extract is prepared from by following method: get fresh cordate houttuynia, the ratio of 10g water is added in every 1g cordate houttuynia, add water, decoct 3 times, each 1.5 hours, merge decoction liquor, filter, dry, to obtain final product.
4. a kind of composition with attracting effect according to claim 3, is characterized in that described composition is made up of the material of following weight portion: silver carp gill extract 0.8 part, houttuynia extract 2 parts, beach wormwood oil 0.2 part.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107624998A (en) * | 2017-11-07 | 2018-01-26 | 广西吉朋投资有限公司 | One breeding hen feed phagostimulant |
CN107668370A (en) * | 2017-09-30 | 2018-02-09 | 广西吉朋投资有限公司 | A kind of phagostimulant for duck feed |
CN107751652A (en) * | 2017-11-29 | 2018-03-06 | 孙铭岐 | A kind of grouper phagostimulant |
Citations (1)
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CN102051399A (en) * | 2009-11-10 | 2011-05-11 | 浙江省海洋开发研究院 | Method for extracting blood pressure lowering peptide from tuna |
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2016
- 2016-01-21 CN CN201610040042.7A patent/CN105558434A/en active Pending
Patent Citations (1)
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CN102051399A (en) * | 2009-11-10 | 2011-05-11 | 浙江省海洋开发研究院 | Method for extracting blood pressure lowering peptide from tuna |
Non-Patent Citations (2)
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107668370A (en) * | 2017-09-30 | 2018-02-09 | 广西吉朋投资有限公司 | A kind of phagostimulant for duck feed |
CN107624998A (en) * | 2017-11-07 | 2018-01-26 | 广西吉朋投资有限公司 | One breeding hen feed phagostimulant |
CN107751652A (en) * | 2017-11-29 | 2018-03-06 | 孙铭岐 | A kind of grouper phagostimulant |
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