CN106577536A - Cultivation method for preventing grass carp enteritis disease - Google Patents
Cultivation method for preventing grass carp enteritis disease Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/58—Meliaceae (Chinaberry or Mahogany family), e.g. Azadirachta (neem)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
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- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/18—Magnoliophyta (angiosperms)
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Abstract
The invention discloses a cultivation method for preventing a grass carp enteritis disease. The cultivation method for preventing the grass carp enteritis disease comprises the following steps that firstly, a cultivation pond is managed, wherein a 70-80 degree angle is formed on the straight bottom face of the cultivation pond and the side face where a water outlet is located, a U-shaped groove with the diameter being 20-30 cm is deep excavated in the joint of the bottom face of the cultivation pond and the side face where the water outlet is located, a double-layer filter screen is arranged on a water inlet, and a herb medicine bag with the weight being 200-300 g is placed in the middle of the double-layer filter screen; secondly, mixed cultivation management is performed, wherein 6000-8000 aquatic plants are grown at the bottom of the cultivation pond, flowing water with the height being 1.8-2.2 m is injected into the pond 42-50 days after growth of the aquatic plants, 10000 to 20000 grass carp fry is bred in the pond, 5-10 kg of a composite anti-disease agent is sprayed in the pond every other 20-25 days, and grass carps are driven for 30-45 min every day with a fish driving device; and thirdly, diseased fish is managed. The cultivation method for the grass carps can enhance the immunity and disease resistance of the grass carps, and therefore the enteritis disease is effectively prevented and treated.
Description
【Technical field】
The present invention relates to the cultivation field of Ctenopharyngodon idellus, more particularly to a kind of cultural method of anti-enteritis disease of grass carp.
【Background technology】
Ctenopharyngodon idellus are one of main freshwater breed varieties, have the advantages that to grow fast, meat flavour deliciousness, and Ctenopharyngodon idellus are careless for food
Animal, is improved seeds that many raisers like cultivating, but Ctenopharyngodon idellus disease is more, survival rate is not high, larger to Ctenopharyngodon idellus harm,
Modal should be the enteritis disease of Ctenopharyngodon idellus, and enteritis disease is one of old three disease of Ctenopharyngodon idellus, all can cause huge to raiser every year
Economic loss.
The enteritis disease Producing reason of Ctenopharyngodon idellus is：Indivedual raiser, especially new raisers, without veritably understanding and the palm
The growth rhythm of Ctenopharyngodon idellus and the self-condition in pond are held, such as water filling condition is poor, water level is shallow, area is excessive or too small, oxygen increasing equipment
Not enough, new water matter is thin, Lao Chi mud is too deep, and eutrophication or the long-term composite factor such as low of aging, dissolved oxygen, still
Growth is pursued simply at high speed, harvests high yield.Take be fed to 12 points it is full in combination with High-energy high-protein feedstuff is fed
Breeding way, the phase intestinal of making too is filled for a long time, makes digestive function all the time in fever in the state of " excited ".Long and
Long it, causes Ctenopharyngodon idellus gastrointestinal tract mechanical injuries.
Their early stage, when feeding feedstuff, there is first " scampering " in pond fish, i.e., in the case of without agitation, group fish is received suddenly
Frightened, moment pierces bottom of pond or leaps up to flight.Continue to feed, can successively return to again in food field and alarmedly rob food, be constantly occurring again
" scampering ".Group fish cannot normally ingest when serious.There is death within one week or so, often first dead larger individuality.Outward appearance disease Air Bladder pseudosciaenae seu Acipenser
Fat meat is expired, without notable condition of illness, only because suffering from enteritis after the serious dehydration of fish body, enophthalmos,enophthalmuses are particularly evident.The course of disease is slightly long, and body colour is sent out
Black, indivedual tail fin tips turn white, and peel off solely trip.Minority has " red skin ", most concurrent with " gill rot " and " liver and gallbladder syndrome " etc.
Disease.The microscopy fish gill and body surface majority are without parasite.Visible volume redness ascites is dissected, shadow about can after the most coverings by fat of intestinal
See, the rubescent inflammation of outward appearance intestinal leading portion, cut that intestinal is all congested, bleeding open, the whole intestinal of serious (bacterial infection) is all congested, go out
Blood, or even suppurate, liver color and luster is thin out or greening, gollbladder dilation.Fish body now is seriously become thin, bone lattice profile protrusion.
" good fish " in random sampling observation morbidity pond, most visible intestinal leading portions are second largest crook, congested and inflammation.Ctenopharyngodon idellus intestinal
What scorching disease was recovered show as, and food is active, eyeball is normal, not only trip, anus not evagination, without red and swollen and abdominal cavity without hydrops, gently press abdomen
Weak yellow liquid is not flowed out during portion from anus.
It is current for the main method of preventing and treating of enteritis disease of grass carp is to be sprinkled in water using control water body and chemical substance, adopt
The mode for cancelling antibacterial in the water that goes out prevents and treats enteritis disease of grass carp, also thus results in chemical substance consumption excessively, easy Ctenopharyngodon idellus body interiorization
Learn material residual excessive, and then affect to eat the healthy of crowd, therefore, the preventing and treating of the enteritis disease of Ctenopharyngodon idellus is studied, improve grass
Resistances against diseases of the fish and cultural technique for not affecting edible crowd healthy has large market using value.
【The content of the invention】
Present invention aim at providing a kind of cultural method of anti-enteritis disease of grass carp, the technology in water inlet by setting again
Medical herbs bag is put, is slowly discharged the siccative medical herbs in medical herbs bag using the impulsive force at water source, kill the antibacterial in water, promote Ctenopharyngodon idellus
Digestion；The present invention at culturing pool bottom also by cultivating pasture and water, the motor capacity of reinforcing Ctenopharyngodon idellus and taking sick fish isolation cultivation
Technological means, strengthen the immunity and premunition of Ctenopharyngodon idellus, so as to effectively prevent and treat enteritis disease.
To reach above-mentioned purpose, the technical solution adopted in the present invention is：A kind of cultural method of anti-enteritis disease of grass carp, bag
Include following steps：
(1) culturing pool management：Soil is deep-cut at water source 15-20m, it is 2.2-2.5m to form depth, and length is 55-
75m, width is the rectangle culturing pool of 35-50m, and in the centre of the broadside of culturing pool water inlet and outlet are respectively equipped with, and supports
Grow that bottom of pond face is straight and side that be located with outlet is in 70-80 degree angle, the side being located in culturing pool bottom surface and outlet
The Baltimore groove of an a diameter of 20-30cm is deep-cut in junction, and the flow rate for keeping cultivation pool inner water is 500-800cm3/ min,
Double-layer leaching net is provided with water inlet, the medical herbs bag that weight is 200-300g is placed with the middle of double-layer leaching net；
(2) management is raised together with：In the composting fertilizer that bottom of pond layer overlay thickness is 2-4cm, exposure 15- after culturing pool digs
20 days, injecting height was the running water of 10-20cm toward in pond, then plants 6000-8000 strain pasture and water at culturing pool bottom, treated that pasture and water give birth to
After long 42-50 days, injecting height is the running water of 1.8-2.2m toward in pond, the tail fries of careless 1-2 ten thousand is put in a suitable place to breed in pond, every 20-25
It sprays the compound antiviral agents of 5-10kg into pond, drives equipment with fish daily and drives Ctenopharyngodon idellus 30-45min；
(3) sick fish management：When finding that anus evagination is red and swollen in cultivation pool side, belly expands, hydroabdomen, during light pressure abdominal part
The sick fish of weak yellow liquid is flowed out from anus, is pulled out immediately, be put into the disappearing for 0.005-0.008% antibacterial containing mass fraction
Cultivate 5-7 days in malicious pond, being then put into isolation tank carries out isolation cultivation 10-15 days, just can return within 2-3 days more afterwards in sick fish diseasess foster
Grow pond to continue to cultivate.
In the present invention, as further illustrating, the medical herbs bag described in step (1) is prepared by following methods：By weight
Number meter, by 25-40 part Folium Artemisiae Argyis, 10-20 part Flos Loniceraes, 13-17 part bitter fruit leafs, 8-15 part Fructus Forsythiaes, 5-12 part Fericarpium Citri Limoniaes, 15-
26 parts of eucalyptus leaveses, 6-14 part Cortex cinnamomi japonici (Ramulus Cinnamomi)s, 6-10 part Herba Senecionis Scandentiss, 8-16 parts Herba Taraxaci and the mixing chopping of 12-30 parts Folium Acori graminei, in 120-
Heat treatment 20-35s under the conditions of 135 DEG C of high-temperature steam, in being fitted into Filter-screen bag, obtains medical herbs bag.
In the present invention, as further illustrating, the pasture and water described in step (2) are in black charophyta, wild Pedicellus et Pericarpium Trapae and watermifoil
One or more.
In the present invention, as further illustrating, step (2) the compound antiviral agents are 300-500 by weight ratio：15-
25：The lactic acid bacteria bacteria suspension of 6-11, lysozyme and beta-1,3-glucanase are mixed.
In the present invention, as further illustrating, the antibacterial described in step (3) is 13-28 by weight ratio：25-43：3-
7：The nitrofural of 15-37, Sucus Zingberis, sodium hypochlorite and water are mixed.
In the present invention, as further illustrating, the aperture of described Filter-screen bag is 1-5mm.
In the present invention, as further illustrating, the concentration of described lactic acid bacteria bacteria suspension is 106-108cfu/mL。
The function introduction of part material is as follows：
Various pathomycetes are in vitro also had certain inhibitory action by Folium Artemisiae Argyi, Chinese mugwort flooding agent and decoct.In the present invention
In, Folium Artemisiae Argyi is used as the raw material for preparing medical herbs bag.
Flos Lonicerae, to various pathogens such as staphylococcus aureuses, Hemolytic streptococcuss, escherichia coli, dysentery bacterium, suddenly
Random vibrio, Bacillus typhi, Salmonella paratyphi etc. have certain inhibitory action, to streptococcus pneumoniae, meningococcus, green pus bar
Bacterium, tubercule bacillus are also effective.Flooding agent is stronger than decoct effect, and leaf decoction is stronger than the effect of colored decoct.In the present invention, Flos Lonicerae is used
Prepare the raw material of medical herbs bag.
Bitter fruit leaf, the incomplete purified of Folium Meliae can suppress virus to the infection of mammalian cell and kill thin
Bacterium.In the present invention, bitter fruit leaf is used as the raw material for preparing medical herbs bag.
Fructus Forsythiae, there is in vitro antibacterial action, can suppress Bacillus typhi, Salmonella paratyphi, escherichia coli, dysentery bacterium, white
Larynx bacillus and vibrio cholera, staphylococcuses, streptococcus etc..In the present invention, Fructus Forsythiae is used as the raw material for preparing medical herbs bag.
Fericarpium Citri Limoniae, is dry Fructus Citri Limoniae peel.The primary efficacy of Fericarpium Citri Limoniae is diuresis, anti-inflammatory, antihistaminic, enhancement food
Desire, stimulation.In the present invention, Fericarpium Citri Limoniae is used as the raw material for preparing medical herbs bag.
Eucalyptus leaveses, there is inhibitory action to gram-positive bacterium, in vitro to clostridium tetani and diphtheria corynebacterium toxin
There is detoxicating adhesive.In the present invention, eucalyptus leaveses are used as the raw material for preparing medical herbs bag.
Cortex cinnamomi japonici (Ramulus Cinnamomi), containing volatile oil, wherein having phellandrene, eugenol, cinnamic aldehyde, eugenol, safrole etc..Cinnamomum japonica and
Burmannii can the secretion of facilitating digestion liquid and exclusion intestinal gas.Eugenol is to staphylococcus aureuses, dysentery bacterium, large intestine bar
Bacterium, Bacillus proteuss etc. have inhibitory action.In the present invention, Cortex cinnamomi japonici (Ramulus Cinnamomi) is used as the raw material for preparing medical herbs bag.
Herba Senecionis Scandentiss, have stronger antibacterial action to will Hayes dysentery bar mattress and staphylococcus aureuses.In the present invention, a thousand li
Light is used as the raw material for preparing medical herbs bag.
Staphylococcus aureus resistance bacterial strain, Hemolytic streptococcuss are had stronger bactericidal action by Herba Taraxaci, double to pneumonia
Coccus, meningococcuss, diphtheria corynebacterium, bacillus pyocyaneus, Bacillus proteuss, dysentery bacterium, Bacillus typhi etc. and micrococcus catarrhalises also have
Certain bactericidal action.In the present invention, Herba Taraxaci is used as the raw material for preparing medical herbs bag.
Folium Acori graminei, is the leaf of acorus gramineus araceae plant.In the present invention, Folium Artemisiae Argyi is used as the raw material for preparing medical herbs bag.
Black charophyta, wild Pedicellus et Pericarpium Trapae and watermifoil are in the present invention as the pasture and water planted in freshwater aquiculture pond.
Lactic acid bacteria, lactic acid bacteria refer to fermenting carbohydrate primary product be lactic acid a class without spore, gram-positive bacterium
General name.Lactic acid bacteria is that a class can utilize fermentable carbohydrate to produce the common name of the antibacterial of a large amount of lactic acid.In the present invention,
Lactic acid bacteria is used to prepare compound antiviral agents, can not only kill the antibacterial in water and provide beneficial microbe for Ctenopharyngodon idellus digestion intestinal
Group, strengthens the digestive system of Ctenopharyngodon idellus.
Lysozyme, is energy that a molecular weight is 14.4kDa also known as muramidase or N-acetylmuramide lycanohydrlase
The alkaline enzyme of mucopolysaccharide in hydrolysis pathogenic bacterium.-acetylmuramic acid and NAG in the main cell wall by destruction
Between β-Isosorbide-5-Nitrae glycosidic bond, make the insoluble mucopolysaccharide of cell wall resolve into solubility glycopeptide, cause cell wall rupture content escape
Go out and make bacterolysises.Lysozyme can also be with negatively charged virus protein directly in conjunction with DNA, RNA, apoprotein shape
Into double salt, make virally inactivated.In the present invention, lysozyme is used to prepare compound antiviral agents, kills the antibacterial in water.
β -1,3- glucanase can suppress the growth of the external disease-resistant original, have resistant effect to biology.In the present invention
In, β -1,3- glucanase is used to prepare compound antiviral agents, kills the antibacterial in water.
Nitrofural, is lemon yellow grain powder.Nitrofural can disturb the carbohydrate metabolism process and oxygen of antibacterial
Change enzyme system and play antibacterial or bactericidal action, the glycometabolic early stage of main interference antibacterial, cause bacterial metabolism disorderly and
It is dead.In the present invention, nitrofural is the primary raw material for preparing antibacterial.
Sucus Zingberis, fresh ginger is pruned crust with knife, is cut to the juice being squeezed into after thin slice, the effect with sterilization.In the present invention
In, Sucus Zingberis are the raw material for preparing antibacterial.
A kind of sodium hypochlorite, strong oxidizer can kill the pathogenic bacteria in water.In the present invention, sodium hypochlorite sterilizes to prepare
The raw material of agent.
The invention has the advantages that：
1. the present invention arranges medical herbs bag in water inlet, is delayed in the haylage medical herbs in medical herbs bag using the impulsive force at water source
On The Drug Release, as current are dissolved in after culturing pool, can will enable the herbal substances in medical herbs bag after substance decomposition in medical herbs bag
Uniformly it is discharged in culturing pool, the pathogenic bacteria in water is killed improves the water quality of culturing pool, improves the immunity of Ctenopharyngodon idellus and disease-resistant
While power, stimulate the formation of microbiologic population in the digestive tract of Ctenopharyngodon idellus, promote the growth of Ctenopharyngodon idellus.Hardship in one side medical herbs bag
In volatile oil and eucalyptus leaveses in the chlorogenic acid, the Folium Acori graminei that all contain in Azedarachin, Folium Artemisiae Argyi and Flos Lonicerae in chinaberry leaf
Citronellal can efficiently kill the antibacterial in water as compound disinfectant, and the alkaloid alkali in Herba Senecionis Scandentiss can increase multiple
The activity of antibacterial is closed, the efficiency of sterilization is improved, the pathogenic bacteria in water is efficiently killed, improves the water quality and microorganism ring of culturing pool
Border, improves the premunition of Ctenopharyngodon idellus；On the other hand, the citric acid of the Fericarpium Citri Limoniae in medical herbs bag, malic acid can promote to stimulate Ctenopharyngodon idellus
The formation of microbiologic population in digestive tract, so as to further speed up the growth of Ctenopharyngodon idellus, and the triterpeness in Flos Lonicerae can promote
The dissolution of citric acid and malic acid, the growth of microbiologic population in further promotion Ctenopharyngodon idellus digestive tract, and then raising Ctenopharyngodon idellus are supported
The ability of anti-enteritis disease.Inside digestive system of the Chinese herbal medicine in medical herbs bag from the external living environment of Ctenopharyngodon idellus and Ctenopharyngodon idellus body this
Two aspects are set about, the microbial environment for improving Ctenopharyngodon idellus from inside to outside, and it is anti-that the effect of generation is far longer than that single composition produces
The summation of condition of disease power, so as to greatly enhance the immunity of Ctenopharyngodon idellus, enhances resistances against diseases of the Ctenopharyngodon idellus to enteritis disease.
2. the compound antiviral agents that prepared by the present invention are pure natural, nuisanceless preparation, can efficiently kill cultivation pool inner water
While middle antibacterial, promote the gastrointestinal disturbances of Ctenopharyngodon idellus, the digestive system for making Ctenopharyngodon idellus to be strengthened, and then improve the anti-enteritis disease of Ctenopharyngodon idellus
Ability.The lysozyme adopted in compound antiviral agents can pass through the -acetylmuramic acid in destruction cell wall and N- acetylaminos Portugal
β between sugar-Isosorbide-5-Nitrae glycosidic bond, makes the insoluble mucopolysaccharide of cell wall resolve into solubility glycopeptide, causes cell wall rupture content
Escape and make bacterolysises, lysozyme can also be with negatively charged virus protein directly in conjunction with DNA, RNA, apoprotein
Double salt is formed, is made virally inactivated；β -1,3- glucanase can suppress the growth of antibacterial, promote dissolving speed of the lysozyme to antibacterial
Degree；And lactic acid bacteria can not only kill the pathogenic bacteria in water, moreover it is possible to promote the gastrointestinal disturbances of Ctenopharyngodon idellus, the digestive system for making Ctenopharyngodon idellus is obtained
Reinforcing, and then improve the ability of the anti-enteritis disease of Ctenopharyngodon idellus；This three external growth ring from different perspectives with different levels to Ctenopharyngodon idellus
The digestive system of border and inside is improved, and substantially increases resistivity of the Ctenopharyngodon idellus to enteritis disease, and the effect that it is produced is far high
In the effect produced by single composition simple plus and.
3. the present invention makes full use of the breeding spaces of culturing pool, takes the mixed breed of Ctenopharyngodon idellus and pasture and water, has using pasture and water
Effect ground absorbs the harmful substances such as ammoniacal nitrogen, hydrogen sulfide, improves the ecosystem of culturing pool, promotes organic matter circling, purified water
Matter, strengthens the immune system of Ctenopharyngodon idellus, improves the immunity and premunition of Ctenopharyngodon idellus.The present invention, by planting pasture and water, is not only Ctenopharyngodon idellus
There is provided the careless feedstuff of appropriateness, moreover it is possible to the harmful substance such as effective ammoniacal nitrogen, hydrogen sulfide absorbed in water, promote microorganism in water
Development, and the development of microorganism further extends the feeding scope of fish in water, promotes the growth of fish, and then improves fish and exempt from
Epidemic disease power and premunition.
4. the body of Ctenopharyngodon idellus is improved while antibacterial of the present invention can efficiently kill the pathogenic bacteria in Ctenopharyngodon idellus body
Quality, makes Ctenopharyngodon idellus reply health as early as possible.Nitrofural in antibacterial can disturb the carbohydrate metabolism process and oxidase system of pathogenic bacteria,
So as to play the effect for killing pathogenic bacteria；Polyphenol powde in Sucus Zingberis stimulates the heart and blood vessel of Ctenopharyngodon idellus while antibacterial is killed, it
The heartbeat that Ctenopharyngodon idellus can be made is accelerated, vasodilation, and blood flow accelerates, and is excreted toxin by the fish gill, and then improves
The premunition of Ctenopharyngodon idellus；And sodium hypochlorite is strong oxidizer and antibacterial, can form weakly alkaline in water while sterilization
Environment, and weakly alkaline environment can improve the germicidal efficiency of nitrofural.This several composition in antibacterial cooperate, mutually
Effect, it is common to improve the effect for killing pathogenic bacteria in Ctenopharyngodon idellus body.
5. the present invention adopts slope design to culturing pool ground, the Excreta of Ctenopharyngodon idellus is enable along inclined-plane, in water flowing
In the presence of, the Excreta for promoting Ctenopharyngodon idellus is scrolled down through along the gradient, then collects these Excretas by the design of groove so that
The easy centralized collection of these Excretas and be cleared out, and then reduce pollution of the Ctenopharyngodon idellus Excreta to water quality, increase water quality,
For the water environment that Ctenopharyngodon idellus build a health.
【Specific embodiment】
Embodiment 1：
1. early-stage preparations
The preparation of medical herbs bag：Count by weight, by 25 parts of Folium Artemisiae Argyis, 10 portions of Flos Loniceraes, 13 parts of bitter fruit leafs, 8 parts of Fructus Forsythiaes,
5 parts of Fericarpium Citri Limoniaes, 15 parts of eucalyptus leaveses, 6 parts of Cortex cinnamomi japonici (Ramulus Cinnamomi)s, 6 parts of Herba Senecionis Scandentiss, 8 parts of Herba Taraxacis and 12 parts of Folium Acori graminei mixing choppings, at 120 DEG C
High-temperature steam under the conditions of heat treatment 20s, in being fitted into Filter-screen bag, obtain medical herbs bag.
The preparation of compound antiviral agents：Count by weight, be 10 by 300 parts of mass fractions6The lactic acid bacteria bacterium of cfu/mL is hanged
Liquid, 15 parts of lysozyme and 6 parts of β -1,3- glucanase mixing obtains compound antiviral agents.
The preparation of antibacterial：Count by weight, 13 parts of nitrofurals, 25 parts of Sucus Zingberis, 3 parts of sodium hypochlorite and 15 water are mixed
Close, stir, obtain antibacterial.
Above-mentioned prepared material is used for into the cultural method of following described anti-enteritis disease of grass carp.
2. a kind of cultural method of anti-enteritis disease of grass carp, comprises the following steps：
(1) culturing pool management：Soil is deep-cut at water source 15m, it is 2.2m to form depth, and length is 55m, and width is
The rectangle culturing pool of 35m, in the centre of the broadside of culturing pool water inlet and outlet are respectively equipped with, culturing pool bottom surface is straight and
It is in 70 degree of angles with the side that outlet is located, a diameter is deep-cut in the junction of the side being located in culturing pool bottom surface and outlet
For the Baltimore groove of 20cm, the flow rate for keeping cultivation pool inner water is 500cm3/ min, in water inlet double-layer leaching net is provided with, double-deck
The medical herbs bag that weight is 200g is placed with the middle of filter screen；
(2) management is raised together with：In the composting fertilizer that bottom of pond layer overlay thickness is 2cm after culturing pool digs, exposure 15 days,
Injecting height is the running water of 10cm toward in pond, then plants 6000 plants of black charophytas at culturing pool bottom, past after pasture and water grow 42 days
Injecting height is the running water of 1.8m in pond, puts careless 10,000 tail fries in a suitable place to breed in pond, sprayed into pond every 20 days 5kg be combined it is disease-resistant
Agent, drives equipment and drives Ctenopharyngodon idellus 30min with fish daily；
(3) sick fish management：When finding that anus evagination is red and swollen in cultivation pool side, belly expands, hydroabdomen, during light pressure abdominal part
The sick fish of weak yellow liquid is flowed out from anus, is pulled out immediately, be put into containing in the sterilization pool that mass fraction is 0.005% antibacterial
Cultivation 5 days, being then put into isolation tank carries out isolation cultivation 10 days, continues to cultivate in the rear culturing pool that just can return to for 2 days of sick fish diseasess.
Embodiment 2：
1. early-stage preparations
The preparation of medical herbs bag：Count by weight, by 26 parts of Folium Artemisiae Argyis, 13 portions of Flos Loniceraes, 14 parts of bitter fruit leafs, 10 parts of companies
Stick up, 6 parts of Fericarpium Citri Limoniaes, 20 parts of eucalyptus leaveses, 8 parts of Cortex cinnamomi japonici (Ramulus Cinnamomi)s, 7 parts of Herba Senecionis Scandentiss, 12 parts of Herba Taraxacis and 25 parts of Folium Acori graminei mixing are shredded,
Heat treatment 28s under the conditions of 133 DEG C of high-temperature steam, in being fitted into Filter-screen bag, obtains medical herbs bag.
The preparation of compound antiviral agents：Count by weight, be 10 by 350 parts of mass fractions7The lactic acid bacteria bacterium of cfu/mL is hanged
Liquid, 20 parts of lysozyme and 7 parts of β -1,3- glucanase mixing obtains compound antiviral agents.
The preparation of antibacterial：Count by weight, 24 parts of nitrofurals, 30 parts of Sucus Zingberis, 4 parts of sodium hypochlorite and 23 water are mixed
Close, stir, obtain antibacterial.
Above-mentioned prepared material is used for into the cultural method of following described anti-enteritis disease of grass carp.
2. a kind of cultural method of anti-enteritis disease of grass carp, comprises the following steps：
(1) culturing pool management：Soil is deep-cut at water source 17m, it is 2.4m to form depth, and length is 60m, and width is
The rectangle culturing pool of 44m, in the centre of the broadside of culturing pool water inlet and outlet are respectively equipped with, culturing pool bottom surface is straight and
It is in 74 degree of angles with the side that outlet is located, a diameter is deep-cut in the junction of the side being located in culturing pool bottom surface and outlet
For the Baltimore groove of 22cm, the flow rate for keeping cultivation pool inner water is 550cm3/ min, in water inlet double-layer leaching net is provided with, double-deck
The medical herbs bag that weight is 260g is placed with the middle of filter screen；
(2) management is raised together with：In the composting fertilizer that bottom of pond layer overlay thickness is 2.5cm, exposure 17 after culturing pool digs
My god, injecting height is the running water of 13cm toward in pond, then plants 6000 plants of black charophytas and 1000 plants of wild Pedicellus et Pericarpium Trapaes at culturing pool bottom, treats
After pasture and water grow 45 days, injecting height is the running water of 2m toward in pond, careless 1.2 ten thousand tail fries is put in a suitable place to breed in pond, every 21 days to pond
Interior sprinkling 6kg is combined antiviral agents, drives equipment with fish daily and drives Ctenopharyngodon idellus 38min；
(3) sick fish management：When finding that anus evagination is red and swollen in cultivation pool side, belly expands, hydroabdomen, during light pressure abdominal part
The sick fish of weak yellow liquid is flowed out from anus, is pulled out immediately, be put into containing in the sterilization pool that mass fraction is 0.006% antibacterial
Cultivation 6 days, being then put into isolation tank carries out isolation cultivation 12 days, continues to support in the rear culturing pool that just can return to for 2.5 days of sick fish diseasess
Grow.
Embodiment 3：
1. early-stage preparations
The preparation of medical herbs bag：Count by weight, by 36 parts of Folium Artemisiae Argyis, 14 portions of Flos Loniceraes, 15 parts of bitter fruit leafs, 10 parts of companies
Stick up, 6 parts of Fericarpium Citri Limoniaes, 24 parts of eucalyptus leaveses, 8 parts of Cortex cinnamomi japonici (Ramulus Cinnamomi)s, 8 parts of Herba Senecionis Scandentiss, 14 parts of Herba Taraxacis and 18 parts of Folium Acori graminei mixing are shredded,
Heat treatment 30s under the conditions of 130 DEG C of high-temperature steam, in being fitted into Filter-screen bag, obtains medical herbs bag.
The preparation of compound antiviral agents：Count by weight, be 10 by 400 parts of mass fractions6The lactic acid bacteria bacterium of cfu/mL is hanged
Liquid, 20 parts of lysozyme and 7 parts of β -1,3- glucanase mixing obtains compound antiviral agents.
The preparation of antibacterial：Count by weight, 26 parts of nitrofurals, 30 parts of Sucus Zingberis, 5 parts of sodium hypochlorite and 20 water are mixed
Close, stir, obtain antibacterial.
Above-mentioned prepared material is used for into the cultural method of following described anti-enteritis disease of grass carp.
2. a kind of cultural method of anti-enteritis disease of grass carp, comprises the following steps：
(1) culturing pool management：Soil is deep-cut at water source 16m, it is 2.4m to form depth, and length is 60m, and width is
The rectangle culturing pool of 40m, in the centre of the broadside of culturing pool water inlet and outlet are respectively equipped with, culturing pool bottom surface is straight and
It is in 77 degree of angles with the side that outlet is located, a diameter is deep-cut in the junction of the side being located in culturing pool bottom surface and outlet
For the Baltimore groove of 26m, the flow rate for keeping cultivation pool inner water is 680cm3/ min, in water inlet double-layer leaching net is provided with, double-deck
The medical herbs bag that weight is 245g is placed with the middle of filter screen；
(2) management is raised together with：In the composting fertilizer that bottom of pond layer overlay thickness is 2.5cm, exposure 18 after culturing pool digs
My god, toward in pond injecting height for 13cm running water, then culturing pool bottom plant 5000 plants of black charophytas, 1000 plants of wild Pedicellus et Pericarpium Trapaes and
1000 plants of watermifoils, after pasture and water grow 46 days, injecting height is the running water of 2.1m toward in pond, puts careless 1.7 ten thousand tails in a suitable place to breed in pond
Fry, the compound antiviral agents of 6kg were sprayed every 21 days into pond, were driven equipment with fish daily and were driven Ctenopharyngodon idellus 40min；
(3) sick fish management：When finding that anus evagination is red and swollen in cultivation pool side, belly expands, hydroabdomen, during light pressure abdominal part
The sick fish of weak yellow liquid is flowed out from anus, is pulled out immediately, be put into containing in the sterilization pool that mass fraction is 0.007% antibacterial
Cultivation 6 days, being then put into isolation tank carries out isolation cultivation 13 days, continues to support in the rear culturing pool that just can return to for 2.5 days of sick fish diseasess
Grow.
Embodiment 4：
1. early-stage preparations
The preparation of medical herbs bag：Count by weight, by 34 parts of Folium Artemisiae Argyis, 15 portions of Flos Loniceraes, 14 parts of bitter fruit leafs, 13 parts of companies
Stick up, 7 parts of Fericarpium Citri Limoniaes, 22 parts of eucalyptus leaveses, 9 parts of Cortex cinnamomi japonici (Ramulus Cinnamomi)s, 8 parts of Herba Senecionis Scandentiss, 12 parts of Herba Taraxacis and 27 parts of Folium Acori graminei mixing are shredded,
Heat treatment 30s under the conditions of 130 DEG C of high-temperature steam, in being fitted into Filter-screen bag, obtains medical herbs bag.
The preparation of compound antiviral agents：Count by weight, be 10 by 470 parts of mass fractions8The lactic acid bacteria bacterium of cfu/mL is hanged
Liquid, 23 parts of lysozyme and 9 parts of β -1,3- glucanase mixing obtains compound antiviral agents.
The preparation of antibacterial：Count by weight, 24 parts of nitrofurals, 38 parts of Sucus Zingberis, 5 parts of sodium hypochlorite and 30 water are mixed
Close, stir, obtain antibacterial.
Above-mentioned prepared material is used for into the cultural method of following described anti-enteritis disease of grass carp.
2. a kind of cultural method of anti-enteritis disease of grass carp, comprises the following steps：
(1) culturing pool management：Soil is deep-cut at water source 18m, it is 2.3m to form depth, and length is 60m, and width is
The rectangle culturing pool of 42m, in the centre of the broadside of culturing pool water inlet and outlet are respectively equipped with, culturing pool bottom surface is straight and
It is in 73 degree of angles with the side that outlet is located, a diameter is deep-cut in the junction of the side being located in culturing pool bottom surface and outlet
For the Baltimore groove of 22cm, the flow rate for keeping cultivation pool inner water is 750cm3/ min, in water inlet double-layer leaching net is provided with, double-deck
The medical herbs bag that weight is 240g is placed with the middle of filter screen；
(2) management is raised together with：In the composting fertilizer that bottom of pond layer overlay thickness is 3.5cm, exposure 18 after culturing pool digs
My god, injecting height is the running water of 13cm toward in pond, then plants 6000 wild Pedicellus et Pericarpium Trapaes at culturing pool bottom, after pasture and water grow 49 days,
Injecting height is the running water of 1.9m toward in pond, puts careless 1.6 ten thousand tail fries in a suitable place to breed in pond, sprayed 8kg into pond every 23 days and is combined
Antiviral agents, drive equipment and drive Ctenopharyngodon idellus 39min with fish daily；
(3) sick fish management：When finding that anus evagination is red and swollen in cultivation pool side, belly expands, hydroabdomen, during light pressure abdominal part
The sick fish of weak yellow liquid is flowed out from anus, is pulled out immediately, be put into containing in the sterilization pool that mass fraction is 0.007% antibacterial
Cultivation 6.5 days, being then put into isolation tank carries out isolation cultivation 11 days, continues in the rear culturing pool that just can return to for 2.5 days of sick fish diseasess
Cultivation.
Embodiment 5：
1. early-stage preparations
The preparation of medical herbs bag：Count by weight, by 34 parts of Folium Artemisiae Argyis, 12 portions of Flos Loniceraes, 14 parts of bitter fruit leafs, 10 parts of companies
Stick up, 10 parts of Fericarpium Citri Limoniaes, 17 parts of eucalyptus leaveses, 12 parts of Cortex cinnamomi japonici (Ramulus Cinnamomi)s, 7 parts of Herba Senecionis Scandentiss, 13 parts of Herba Taraxacis and 17 parts of Folium Acori graminei mixing are shredded,
Heat treatment 24s under the conditions of 130 DEG C of high-temperature steam, in being fitted into Filter-screen bag, obtains medical herbs bag.
The preparation of compound antiviral agents：Count by weight, be 10 by 430 parts of mass fractions6The lactic acid bacteria bacterium of cfu/mL is hanged
Liquid, 21 parts of lysozyme and 7 parts of β -1,3- glucanase mixing obtains compound antiviral agents.
The preparation of antibacterial：Count by weight, 26 parts of nitrofurals, 37 parts of Sucus Zingberis, 4 parts of sodium hypochlorite and 26 water are mixed
Close, stir, obtain antibacterial.
Above-mentioned prepared material is used for into the cultural method of following described anti-enteritis disease of grass carp.
2. a kind of cultural method of anti-enteritis disease of grass carp, comprises the following steps：
(1) culturing pool management：Soil is deep-cut at water source 18m, it is 2.4m to form depth, and length is 60m, and width is
The rectangle culturing pool of 47m, in the centre of the broadside of culturing pool water inlet and outlet are respectively equipped with, culturing pool bottom surface is straight and
It is in 76 degree of angles with the side that outlet is located, a diameter is deep-cut in the junction of the side being located in culturing pool bottom surface and outlet
For the Baltimore groove of 23cm, the flow rate for keeping cultivation pool inner water is 720cm3/ min, in water inlet double-layer leaching net is provided with, double-deck
The medical herbs bag that weight is 260g is placed with the middle of filter screen；
(2) management is raised together with：In the composting fertilizer that bottom of pond layer overlay thickness is 3cm after culturing pool digs, exposure 19 days,
Injecting height is the running water of 16cm toward in pond, then plants 6500 watermifoils at culturing pool bottom, after pasture and water grow 44 days, toward pond
Interior injecting height is the running water of 2.2m, puts careless 1.8 ten thousand tail fries in a suitable place to breed in pond, sprayed into pond every 22 days 7kg be combined it is disease-resistant
Agent, drives equipment and drives Ctenopharyngodon idellus 38min with fish daily；
(3) sick fish management：When finding that anus evagination is red and swollen in cultivation pool side, belly expands, hydroabdomen, during light pressure abdominal part
The sick fish of weak yellow liquid is flowed out from anus, is pulled out immediately, be put into containing in the sterilization pool that mass fraction is 0.006% antibacterial
Cultivation 6.5 days, being then put into isolation tank carries out isolation cultivation 14 days, continues in the rear culturing pool that just can return to for 2.5 days of sick fish diseasess
Cultivation.
Embodiment 6：
1. early-stage preparations
The preparation of medical herbs bag：Count by weight, by 40 parts of Folium Artemisiae Argyis, 20 portions of Flos Loniceraes, 17 parts of bitter fruit leafs, 15 parts of companies
Stick up, 12 parts of Fericarpium Citri Limoniaes, 26 parts of eucalyptus leaveses, 14 parts of Cortex cinnamomi japonici (Ramulus Cinnamomi)s, 10 parts of Herba Senecionis Scandentiss, 16 parts of Herba Taraxacis and 30 parts of Folium Acori graminei mixing are shredded,
The heat treatment 35s under the conditions of 135 DEG C of high-temperature steam, in being fitted into Filter-screen bag, obtains medical herbs bag.
The preparation of compound antiviral agents：Count by weight, be 10 by 500 parts of mass fractions8The lactic acid bacteria bacterium of cfu/mL is hanged
Liquid, 25 parts of lysozyme and 11 parts of β -1,3- glucanase mixing obtains compound antiviral agents.
The preparation of antibacterial：Count by weight, 28 parts of nitrofurals, 43 parts of Sucus Zingberis, 7 parts of sodium hypochlorite and 37 water are mixed
Close, stir, obtain antibacterial.
Above-mentioned prepared material is used for into the cultural method of following described anti-enteritis disease of grass carp.
2. a kind of cultural method of anti-enteritis disease of grass carp, comprises the following steps：
(1) culturing pool management：Soil is deep-cut at water source 20m, it is 2.5m to form depth, and length is 75m, and width is
The rectangle culturing pool of 50m, in the centre of the broadside of culturing pool water inlet and outlet are respectively equipped with, culturing pool bottom surface is straight and
It is in 80 degree of angles with the side that outlet is located, a diameter is deep-cut in the junction of the side being located in culturing pool bottom surface and outlet
For the Baltimore groove of 30cm, the flow rate for keeping cultivation pool inner water is 800cm3/ min, in water inlet double-layer leaching net is provided with, double-deck
The medical herbs bag that weight is 300g is placed with the middle of filter screen；
(2) management is raised together with：In the composting fertilizer that bottom of pond layer overlay thickness is 4cm after culturing pool digs, exposure 20 days,
Injecting height is the running water of 20cm toward in pond, then plants 6000 plants of black charophytas, 1000 plants of wild Pedicellus et Pericarpium Trapaes and 1000 at culturing pool bottom
Strain watermifoil, after pasture and water grow 50 days, injecting height is the running water of 2.2m toward in pond, puts careless 20,000 tail fries in a suitable place to breed in pond, often
The compound antiviral agents of 10kg were sprayed into pond every 25 days, equipment is driven with fish daily and is driven Ctenopharyngodon idellus 45min；
(3) sick fish management：When finding that anus evagination is red and swollen in cultivation pool side, belly expands, hydroabdomen, during light pressure abdominal part
The sick fish of weak yellow liquid is flowed out from anus, is pulled out immediately, be put into containing in the sterilization pool that mass fraction is 0.008% antibacterial
Cultivation 7 days, being then put into isolation tank carries out isolation cultivation 15 days, continues to cultivate in the rear culturing pool that just can return to for 3 days of sick fish diseasess.
Comparative example 1：The concrete steps of the method for anti-enteritis disease of grass carp are substantially the same manner as Example 1, and difference is：Step
(1) medical herbs bag is not adopted.
Comparative example 2：The concrete steps of the method for anti-enteritis disease of grass carp are substantially the same manner as Example 1, and difference is：Step
(2) pasture and water are not planted.
Comparative example 3：The concrete steps of the method for anti-enteritis disease of grass carp are substantially the same manner as Example 1, and difference is：Step
(2) without using compound antiviral agents.
Comparative example 4：The concrete steps of the method for anti-enteritis disease of grass carp are substantially the same manner as Example 1, and difference is：Step
(3) antibacterial for adopting is for the u'eno bloom sold on the market.
Contrast test 1：
The method of comparative example 1-4 and embodiment 1-6 is respectively cultivated 20,000 tail Ctenopharyngodon idellus, Ctenopharyngodon idellus Ctenopharyngodon idellus in growth course are detected
The incidence rate of enteritis disease and the cure rate of enteritis disease, calculate testing result, the results are shown in Table 1.
Table 1：
| Enteritis disease incidence rate | Enteritis disease cure rate | |
| Comparative example 1 | 2.2% | 18% |
| Comparative example 2 | 1.6% | 21% |
| Comparative example 3 | 2.1% | 19% |
| Comparative example 4 | 2.0% | 20% |
| Embodiment 1 | 0.33% | 85% |
| Embodiment 2 | 0.32% | 86% |
| Embodiment 3 | 0.31% | 86% |
| Embodiment 4 | 0.21% | 88% |
| Embodiment 5 | 0.24% | 84% |
| Embodiment 6 | 0.25% | 83% |
The result of table 1 shows：Enteritis disease incidence rate is lower, illustrates that the cultural method is more effective, and enteritis disease incidence rate is from low
It is arranged as to height：Embodiment 4<Embodiment 5<Embodiment 6<Embodiment 3<Embodiment 2<Embodiment 1<Comparative example 2<Comparative example 4<It is right
Ratio 3<Comparative example 2；
Enteritis disease cure rate is higher, illustrates that the cultural method is more effective, and enteritis disease cure rate is arranged as from high to low：Implement
Example 4>Embodiment 2=embodiment 3>Embodiment 1>Embodiment 5>Embodiment 6>Comparative example 2>Comparative example 4>Comparative example 3>Comparative example 1.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this
Bright patent claim, the equal change completed under the technical spirit suggested by all present invention or modification change, all should belong to
Cover the scope of the claims in the present invention.
Claims (7)
1. a kind of cultural method of anti-enteritis disease of grass carp, it is characterised in that：Comprise the following steps：
(1) culturing pool management：Soil is deep-cut at water source 15-20m, it is 2.2-2.5m to form depth, and length is 55-75m, wide
The rectangle culturing pool for 35-50m is spent, in the centre of the broadside of culturing pool water inlet and outlet, culturing pool bottom are respectively equipped with
The side that face is straight and is located with outlet is in 70-80 degree angle, the junction of the side being located in culturing pool bottom surface and outlet
The Baltimore groove of an a diameter of 20-30cm is deep-cut, the flow rate for keeping cultivation pool inner water is 500-800cm3/ min, in water inlet
Mouth is provided with double-layer leaching net, and the medical herbs bag that weight is 200-300g is placed with the middle of double-layer leaching net；
(2) management is raised together with：In the composting fertilizer that bottom of pond layer overlay thickness is 2-4cm, exposure 15-20 after culturing pool digs
My god, injecting height is the running water of 10-20cm toward in pond, then plants 6000-8000 strain pasture and water at culturing pool bottom, treats that pasture and water grow
After 42-50 days, injecting height is the running water of 1.8-2.2m toward in pond, the tail fries of careless 1-2 ten thousand is put in a suitable place to breed in pond, every 20-25 days
The compound antiviral agents of 5-10kg are sprayed into pond, equipment is driven with fish daily and is driven Ctenopharyngodon idellus 30-45min；
(3) sick fish management：When finding that anus evagination is red and swollen in cultivation pool side, belly expands, hydroabdomen, from anus during light pressure abdominal part
Door flows out the sick fish of weak yellow liquid, pulls out immediately, is put into the sterilization pool for 0.005-0.008% antibacterial containing mass fraction
Middle cultivation 5-7 days, being then put into isolation tank carries out isolation cultivation 10-15 days, and culturing pool can be just returned within 2-3 days more afterwards in sick fish diseasess
Continue to cultivate.
2. the cultural method of a kind of anti-enteritis disease of grass carp according to claim 1, it is characterised in that：Described in step (1)
Medical herbs bag is prepared by following methods：Count by weight, by 25-40 part Folium Artemisiae Argyis, 10-20 part Flos Loniceraes, 13-17 part margosas
Leaf, 8-15 part Fructus Forsythiaes, 5-12 part Fericarpium Citri Limoniaes, 15-26 part eucalyptus leaveses, 6-14 part Cortex cinnamomi japonici (Ramulus Cinnamomi)s, 6-10 part Herba Senecionis Scandentiss, 8-16 part Herba Taraxacis
Shred with the mixing of 12-30 parts Folium Acori graminei, the heat treatment 20-35s under the conditions of 120-135 DEG C of high-temperature steam, in being fitted into Filter-screen bag,
Obtain medical herbs bag.
3. the cultural method of a kind of anti-enteritis disease of grass carp according to claim 1, it is characterised in that：Described in step (2)
Pasture and water are one or more in black charophyta, wild Pedicellus et Pericarpium Trapae and watermifoil.
4. the cultural method of a kind of anti-enteritis disease of grass carp according to claim 1, it is characterised in that：Step (2) is described multiple
It is 300-500 that antiviral agents are closed by weight ratio：15-25：The mixing of the lactic acid bacteria bacteria suspension of 6-11, lysozyme and beta-1,3-glucanase
Form.
5. the cultural method of a kind of anti-enteritis disease of grass carp according to claim 1, it is characterised in that：Described in step (3)
Antibacterial is 13-28 by weight ratio：25-43：3-7：The nitrofural of 15-37, Sucus Zingberis, sodium hypochlorite and water are mixed.
6. the cultural method of a kind of anti-enteritis disease of grass carp according to claim 2, it is characterised in that：Described Filter-screen bag
Aperture is 1-5mm.
7. the cultural method of a kind of anti-enteritis disease of grass carp according to claim 4, it is characterised in that：Described lactic acid bacteria bacterium
The concentration of suspension is 106-108cfu/mL。
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