CN107135994A - A kind of cultural method of anti-grass carp bacterial enteritis disease - Google Patents
A kind of cultural method of anti-grass carp bacterial enteritis disease Download PDFInfo
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- CN107135994A CN107135994A CN201710296225.XA CN201710296225A CN107135994A CN 107135994 A CN107135994 A CN 107135994A CN 201710296225 A CN201710296225 A CN 201710296225A CN 107135994 A CN107135994 A CN 107135994A
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Classifications
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- A—HUMAN NECESSITIES
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- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
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- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- C12Y—ENZYMES
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Gastroenterology & Hepatology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of cultural method of anti-grass carp bacterial enteritis disease, comprise the following steps：(1) improvement of soil；(2) management is raised together with：Pasture and water are planted in culturing pool, after pasture and water grow 30~40 days, again toward the running water that injecting height in pond is 1.0~1.5m, oral siphon is provided with culturing pool bottom, outlet pipe is provided with the middle part on oral siphon opposite, one water jet oxygen increasing equipment is respectively installed in oral siphon and outlet pipe, water jet 6~8h of oxygenation is carried out daily, then 0.8~1.2 ten thousand tail fries are put in a suitable place to breed in pond, the consumption for weighing 3~5% according to fish body daily feeds grass carp 12 months using feed of grass carp；(3) sick fish management.Cultural method of the present invention can efficiently reduce the incidence of bacterial enteritis disease; while improving the cure rate of bacterial enteritis disease; the content of heavy metal in soil can also significantly be lowered, the technology has significant directive significance for scale, the industrialized development of grass carp.
Description
【Technical field】
The present invention relates to the cultivation field of fish, more particularly to a kind of cultural method of anti-grass carp bacterial enteritis disease.
【Background technology】
Grass carp is one of main freshwater breed variety, has the advantages that to grow fast, meat flavour deliciousness, grass carp is careless for food
Animal, is the improved seeds that many raisers like cultivation, but grass carp disease is more, survival rate is not high, endangers larger to grass carp,
Most common should be the bacterial enteritis disease of grass carp, cause grass carp hemorrhage inflammation, scale to come off, bacterial enteritis disease is grass carp
One of old three disease, can all cause huge economic loss to raiser every year.
The performance symptom of bacterial enteritis disease is：Peel off only swimming fish body of sick fish blacks, anorexia, so that do not eat completely
Food, splits in intestinal tube, intestinal wall contrafluxion, inflammation, enteric cavity no food or only has a small amount of food holding section, intestinal mucus compared with
It is many.Enteron aisle is because extravasated blood is in aubergine when serious, and intestinal wall elasticity is poor, and enteral has a large amount of faint yellow mucus, and anus is red and swollen.By sick fish
Head lift, that is, have yellow mucus from anus flow out.
The current main method of preventing and treating for grass carp bacterial enteritis disease is to be sprinkled into using chemical substance in water, takes and disappears
The mode of bacterium in water of going out prevents and treats grass carp bacterial enteritis disease, also therefore causes chemical substance consumption excessive, in easy grass carp body
Chemical residue is excessive, and then influences edible crowd's healthy, and the chemical engineering sewage due to existing soil and life
The generation of the random discharge phenomenon of waste water, many soil are contaminated by heavy metals, and once with contaminated by heavy metals soil cultured fishes,
Not only it is easily reduced the resistance against diseases of fish, it is also possible to heavy metal is transferred in the body of consumer by food chain, offsets
The body of expense person causes serious infringement.Therefore, a kind of cultural method of anti-grass carp bacterial enteritis disease is studied, grass carp is improved
The resistance against diseases and cultural technique for not influenceing edible crowd healthy has large market application value.
【The content of the invention】
Present invention aims at a kind of cultural method of anti-grass carp bacterial enteritis disease is provided, the technology is first passed through in cultivation
The technological means of ciliate desert-grass is planted in pond, the soil of culturing pool is improved, the content of heavy metal in soil is reduced；Then lead to
Cross and the technological means that pasture and water and grass carp are raised together with is planted in culturing pool, make to form cyclic microorganism system in culturing pool；And
The oral siphon positioned at culturing pool bottom is provided with culturing pool, water jet oxygen increasing equipment is installed in culturing pool, is accelerated in culturing pool
The flowing of water, reduces the temperature difference in culturing pool；Finally by the management to sick fish, raising is controlled grass carp bacterial enteritis disease
Treat.Cultural method of the present invention can efficiently reduce the incidence of bacterial enteritis disease, improve bacillary enteritis
While the cure rate of disease, moreover it is possible to significantly lower the content of heavy metal in soil, scale, industrialization of the technology for grass carp
Development has significant directive significance.
To reach above-mentioned purpose, the technical solution adopted in the present invention is：A kind of cultivation of anti-grass carp bacterial enteritis disease
Method, comprises the following steps：
(1) improvement of soil：Soil is deep-cut, depth is formed for 1.5~1.8m, length is 65~80m, width is 35~
50m culturing pool, then smashes cultivation bottom of pond portion soil block, and be soaked in water soil 15~20 days, then on soil according to 40 ×
60cm specification plantation ciliate desert-grass 2~3 years, removes after ciliate desert-grass, exposure culturing pool 15~20 days；
(2) management is raised together with：At culturing pool bottom, injecting height is 20cm water, is then planted according to 35 × 45cm specification
Pasture and water, after pasture and water grow 30~40 days, then toward the running water that injecting height in pond is 1.0~1.5m, keep the flow rate of water
For 800~1000cm3/ min, oral siphon is provided with culturing pool bottom, is provided with outlet pipe at the middle part on oral siphon opposite, is being entered
Water pipe and outlet pipe respectively install a water jet oxygen increasing equipment, and water jet 6~8h of oxygenation is carried out daily, is then put in a suitable place to breed in pond
0.8~1.2 ten thousand tail fries, the consumption for weighing 3~5% according to fish body daily feeds grass carp 12 months using feed of grass carp；
(3) sick fish management：When cultivation pool side find it is slow in reacting, peel off solely swim in the water surface, body surface is slight congested or goes out
Blood, belly expand the grass carp red and swollen with anus, pull grass carp out immediately, are soaked after 10~15min of sick fish, rinsed with biological disinfectant
Totally, the isolation tank progress isolation cultivation that with the addition of antiviral agents is then put into, can just be returned to after sick fish disease is cured 2~3 days
Culturing pool continues to cultivate.
In the present invention, as further illustrating, the feed of grass carp described in step (3) is prepared by following methods：By weight
Number meter, after 30~51 parts of shepherd's purse leaves, 25~40 parts of sweet potato leaves and 1~3 part of waste mushroom packet are mixed, 24~36h of sealing and fermenting takes
30~60 portions of peanut cakes, 20~40 portions of corn flour, 10~15 parts of bone meal and 2 parts of garlic powders are mixed into after going out, high-voltage pulse dress is put into
In putting, electric-field intensity be 35~70kv/cm, umber of pulse be carry out under conditions of 200~300 high-voltage pulse processing 20~
30min, is blended into 0.3~0.5 part of bifidobacterium species atcc liquid and 1 part of bacillus licheniformis kind liquid, and compression molding after stirring is obtained
To feed of grass carp.
In the present invention, as further illustrating, described waste mushroom packet is mushroom waste mushroom packet, flat mushroom waste mushroom packet, black fungus
Any one in waste mushroom packet and asparagus waste mushroom packet.
In the present invention, as further illustrating, the pasture and water described in step (2) are eel grass, hydrilla verticillata, small najas marina, thin
Any one of the careless and small water banyan of leaf iron imperial crown, mini green pepper.
In the present invention, as further illustrating, the biological disinfectant described in step (3) is by being 20~33 by weight：
350~600：3~8：1000 staphylococcus lysozyme, epsilon-polylysine, acetum and deionized water mixing, pH value is 4.5
~5.2.
In the present invention, as further illustrating, the mass fraction of described acetum is 1~2%.
The function introduction of part material is as follows：
Ciliate desert-grass, be the arsenic content in a kind of nephrolepidaceae plant, ciliate desert-grass up to 1% -2%, and focus mostly on the ground
Part, can improve soil property soil, in the present invention, and ciliate desert-grass is used as the heavy metal in absorption culturing pool, improves the soil of culturing pool
Earth.
Shepherd's purse leaf and sweet potato leaves, in the present invention as the raw material for preparing feed of grass carp.
The mycelium of waste mushroom packet, organic matter and residual, still containing nutrients such as substantial amounts of C, N, in the present invention not only
Nutriment is provided for feed of grass carp, also using microorganism remaining in bacterium bag, promotes the fermentation of sweet potato leaves and shepherd's purse leaf.
Peanut cake, corn flour and bone meal, in the present invention as the raw material for preparing feed of grass carp, provide necessary for grass carp
Nutrient.
Garlic powder, in the present invention for the raw material of preparation grass carp, the premunition for improving grass carp.
Bifidobacterium species atcc liquid and bacillus licheniformis, in the present invention to prepare the raw material of feed of grass carp, for improving grass
The gastrointestinal disturbances ability of fish.
Eel grass, hydrilla verticillata, small najas marina, spire iron imperial crown, the careless and small water banyan of mini green pepper, in the present invention as grass carp
Greenfeed.
Staphylococcus lysozyme, epsilon-polylysine and acetum are used as the raw material for preparing biological disinfectant in the present invention.
The invention has the advantages that：
1. the present invention makes full use of space cultivation technology, using grass carp and pasture and water mixed breed, effectively will using pasture and water
The harmful substances such as ammoniacal nitrogen, hydrogen sulfide absorb, and improve the ecosystem of culturing pool, promote organic matter circling, purify water, and strengthen
The immune system of grass carp, improves the immunity and premunition of grass carp.The present invention provides suitable by planting pasture and water, not only for grass carp
The greenfeed of degree, moreover it is possible to the harmful substance such as effective ammoniacal nitrogen, hydrogen sulfide absorbed in water, promotes the development of microorganism in water,
And in water microorganism development further extend fish feeding scope, promote fish growth, and then improve fish immunity and
Premunition.On the other hand, the formation of the ecosystem can reduce the rate of change of temperature in culturing pool, and then reduce due to temperature
The incidence of grass carp bacterial enteritis disease caused by change is huge.The present invention makes grass carp, pasture and water by scientific and reasonable collocation
Mutually promote, interact with the microorganism in water, the ecosystem of microorganism can balanced hair in this few person's composition fish-grass-water
Exhibition, the effect of its disease resisting effect cultivation more independent than grass carp produced is good.
2. the present invention in culturing pool by installing water jet oxygen increasing equipment and installing the skill of oral siphon in culturing pool bottom
Art means, can not only strengthen the convection current of water body in culturing pool, promote cyclic microorganism, the temperature in culturing pool is greatly reduced
Difference, moreover it is possible to by the water spray area during water spray, increases the irradiated area of sunlight, and then the temperature difference of reduction culturing pool,
Further reduce the generation of the bacterial enteritis disease produced due to huge temperature difference, it is to avoid the micro-pore aeration of existing use
The shortcoming of water circulation is not accelerated in oxygen increasing equipment oxygenation.Simultaneously, the present invention in culturing pool bottom also by installing into water
The technological means of pipe, forces water body to flow from the bottom up, and the strong external force in conjunction with water jet oxygenation agent is promoted, and is further increased
The flow rate of water body, significantly reduces the temperature difference in culturing pool.
3. feed of grass carp prepared by the present invention can be easier by the stomach absorption of grass carp, reduction stomach burden, and then is subtracted
It is few due to grass carp stomach over-burden and the generation of bacterial enteritis disease that causes.The present invention first passes through micro- life in waste mushroom packet
Thing, promotes fermenting raw materials degraded, the macromolecules degradation in raw material is held digestible small molecule into stomach；Then high-tension pulse is passed through
Punching is handled, and is destroyed eucaryotic cell structure in each raw material, the nutriment of intracellular cladding is fully released, and the thing
Matter is easily absorbed by stomach, and then mitigates the burden of stomach；Garlic powder in feed of grass carp can also kill the germ in stomach,
Improve the resistance against diseases of grass carp；Finally by addition bifidobacterium species atcc liquid and bacillus licheniformis, the ecology in grass carp body is maintained
Balance, promotes grass carp for the absorption of nutriment.
, can be using ciliate desert-grass for heavy metal 4. the present invention improves the soil of culturing pool by the way of plantation ciliate desert-grass
High absorption property, the content of heavy metal in soil is greatly reduced, and then a healthy life cycle is provided for the growth of grass carp
Border.
5. biological disinfectant of the present invention can effectively kill the germ colonized on the grass carp gill filament, bacterium is improved
The cure rate of property enteritis disease.The technological means cooperated in the present invention using biological disinfectant and antiviral agents, can be carried significantly
The cure rate of high grass carp bacterial enteritis disease, the letter of effect produced by its general effect produced is significantly larger than single technological means
It is single plus and.
【Embodiment】
Embodiment 1：
1. early-stage preparations：
The preparation of feed of grass carp：Count by weight, 30 parts of shepherd's purse leaves, 25 parts of sweet potato leaves and 1 part of mushroom waste mushroom packet are mixed
After conjunction, sealing and fermenting 24h is mixed into 30 portions of peanut cakes, 20 portions of corn flour, 10 parts of bone meal and 2 parts of garlic powders, is put into high pressure after taking-up
It is that 35kv/cm, umber of pulse are to carry out high-voltage pulse processing 20min under conditions of 200 in electric-field intensity, then mix in pulser
Enter 0.3 part of bifidobacterium species atcc liquid and 1 part of bacillus licheniformis kind liquid, compression molding after stirring obtains feed of grass carp.
The preparation of biological disinfectant：Count by weight, by 20 parts of staphylococcus lysozymes, 350 parts of epsilon-polylysines, 3 parts
Acetum and 1000 part deionized water mixing of the mass fraction for 1%, regulation pH value is 4.5, obtains biological disinfectant.
The material that above-mentioned early stage is prepared is used for following cultural methods.
2. a kind of cultural method of anti-grass carp bacterial enteritis disease, comprises the following steps：
(1) improvement of soil：Soil is deep-cut, depth is formed for 1.5m, length is 65m, width is 35m culturing pool, so
Cultivation bottom of pond portion soil block is smashed afterwards, be soaked in water soil 15 days, then according to 40 × 60cm specification centipede is planted on soil
Grass 2 years, is removed after ciliate desert-grass, exposure culturing pool 15 days；
(2) management is raised together with：At culturing pool bottom, injecting height is 20cm water, is then planted according to 35 × 45cm specification
Eel grass, after eel grass grows 30 days, then toward the running water that injecting height in pond is 1.0m, the flow rate for keeping water is 800cm3/
Min, oral siphon is provided with culturing pool bottom, is provided with outlet pipe at the middle part on oral siphon opposite, is respectively pacified in oral siphon and outlet pipe
Fill a water jet oxygen increasing equipment, water jet oxygenation 6h carried out daily, then put 0.8 ten thousand tail fries in a suitable place to breed in pond, daily according to
The consumption that fish body weighs 3% feeds grass carp 12 months using feed of grass carp；
(3) sick fish management：When cultivation pool side find it is slow in reacting, peel off solely swim in the water surface, body surface is slight congested or goes out
Blood, belly expand the grass carp red and swollen with anus, pull grass carp out immediately, are soaked with biological disinfectant after sick fish 10min, rinse dry
Only, the isolation tank progress isolation cultivation that with the addition of antiviral agents is then put into, culturing pool can be just returned to after sick fish disease is cured 2 days
Continue to cultivate.
Embodiment 2：
1. early-stage preparations：
The preparation of feed of grass carp：Count by weight, by 35 parts of shepherd's purse leaves, 28 parts of sweet potato leaves and 1.5 parts of flat mushroom waste mushroom packets
After mixing, sealing and fermenting 28h is mixed into 37 portions of peanut cakes, 24 portions of corn flour, 12 parts of bone meal and 2 parts of garlic powders, is put into height after taking-up
Press in pulser, be that 40kv/cm, umber of pulse are to carry out high-voltage pulse processing 25min under conditions of 220 in electric-field intensity, then
0.35 part of bifidobacterium species atcc liquid and 1 part of bacillus licheniformis kind liquid are mixed into, compression molding after stirring obtains feed of grass carp.
The preparation of biological disinfectant：Count by weight, by 23 parts of staphylococcus lysozymes, 400 parts of epsilon-polylysines, 4 parts
Acetum and 1000 part deionized water mixing of the mass fraction for 1.2%, regulation pH value is 4.8, obtains biological disinfectant.
The material that above-mentioned early stage is prepared is used for following cultural methods.
2. a kind of cultural method of anti-grass carp bacterial enteritis disease, comprises the following steps：
(1) improvement of soil：Soil is deep-cut, depth is formed for 1.6m, length is 70m, width is 40m culturing pool, so
Cultivation bottom of pond portion soil block is smashed afterwards, be soaked in water soil 18 days, then according to 40 × 60cm specification centipede is planted on soil
Grass 2.5 years, is removed after ciliate desert-grass, exposure culturing pool 16 days；
(2) management is raised together with：At culturing pool bottom, injecting height is 20cm water, is then planted according to 35 × 45cm specification
Hydrilla verticillata, after hydrilla verticillata grows 35 days, then toward the running water that injecting height in pond is 1.1m, the flow rate for keeping water is
850cm3/ min, oral siphon is provided with culturing pool bottom, outlet pipe is provided with the middle part on oral siphon opposite, in oral siphon and water outlet
Pipe one water jet oxygen increasing equipment of each installation, carries out water jet oxygenation 6h, then puts 0.9 ten thousand tail fries in a suitable place to breed in pond, often daily
Its consumption for weighing 3.5% according to fish body uses feed of grass carp to feed grass carp 12 months；
(3) sick fish management：When cultivation pool side find it is slow in reacting, peel off solely swim in the water surface, body surface is slight congested or goes out
Blood, belly expand the grass carp red and swollen with anus, pull grass carp out immediately, are soaked with biological disinfectant after sick fish 14min, rinse dry
Only, the isolation tank progress isolation cultivation that with the addition of antiviral agents is then put into, cultivation can be just returned to after sick fish disease is cured 2.5 days
Continue to cultivate in pond.
Embodiment 3：
1. early-stage preparations：
The preparation of feed of grass carp：Count by weight, by 40 parts of shepherd's purse leaves, 30 parts of sweet potato leaves and the useless bacterium of 1.5 portions of black fungus
After bag mixing, sealing and fermenting 26h is mixed into 45 portions of peanut cakes, 33 portions of corn flour, 11 parts of bone meal and 2 parts of garlic powders, is put into after taking-up
It is that 50kv/cm, umber of pulse are to carry out high-voltage pulse processing 27min under conditions of 240 in electric-field intensity in high-pressure pulse device,
0.4 part of bifidobacterium species atcc liquid and 1 part of bacillus licheniformis kind liquid are blended into, compression molding after stirring obtains feed of grass carp.
The preparation of biological disinfectant：Count by weight, by 30 parts of staphylococcus lysozymes, 450 parts of epsilon-polylysines, 6 parts
Acetum and 1000 part deionized water mixing of the mass fraction for 1.3%, regulation pH value is 4.8, obtains biological disinfectant.
The material that above-mentioned early stage is prepared is used for following cultural methods.
2. a kind of cultural method of anti-grass carp bacterial enteritis disease, comprises the following steps：
(1) improvement of soil：Soil is deep-cut, depth is formed for 1.7m, length is 72m, width is 43m culturing pool, so
Cultivation bottom of pond portion soil block is smashed afterwards, be soaked in water soil 19 days, then according to 40 × 60cm specification centipede is planted on soil
Grass 3 years, is removed after ciliate desert-grass, exposure culturing pool 17 days；
(2) management is raised together with：At culturing pool bottom, injecting height is 20cm water, is then planted according to 35 × 45cm specification
Small najas marina, after small najas marina grows 34 days, then toward the running water that injecting height in pond is 1.4m, the flow rate for keeping water is
900cm3/ min, oral siphon is provided with culturing pool bottom, outlet pipe is provided with the middle part on oral siphon opposite, in oral siphon and water outlet
Pipe one water jet oxygen increasing equipment of each installation, carries out water jet oxygenation 6.5h, then puts 10,000 tail fries in a suitable place to breed in pond, often daily
Its consumption for weighing 4% according to fish body uses feed of grass carp to feed grass carp 12 months；
(3) sick fish management：When cultivation pool side find it is slow in reacting, peel off solely swim in the water surface, body surface is slight congested or goes out
Blood, belly expand the grass carp red and swollen with anus, pull grass carp out immediately, are soaked with biological disinfectant after sick fish 13min, rinse dry
Only, the isolation tank progress isolation cultivation that with the addition of antiviral agents is then put into, culturing pool can be just returned to after sick fish disease is cured 3 days
Continue to cultivate.
Embodiment 4：
1. early-stage preparations：
The preparation of feed of grass carp：Count by weight, by 43 parts of shepherd's purse leaves, 29 parts of sweet potato leaves and 2 parts of asparagus waste mushroom packets
After mixing, sealing and fermenting 30h is mixed into 55 portions of peanut cakes, 35 portions of corn flour, 13 parts of bone meal and 2 parts of garlic powders, is put into height after taking-up
Press in pulser, be that 60kv/cm, umber of pulse are to carry out high-voltage pulse processing 28min under conditions of 270 in electric-field intensity, then
0.43 part of bifidobacterium species atcc liquid and 1 part of bacillus licheniformis kind liquid are mixed into, compression molding after stirring obtains feed of grass carp.
The preparation of biological disinfectant：Count by weight, by 31 parts of staphylococcus lysozymes, 500 parts of epsilon-polylysines, 4 parts
Acetum and 1000 part deionized water mixing of the mass fraction for 1.8%, regulation pH value is 5, obtains biological disinfectant.
The material that above-mentioned early stage is prepared is used for following cultural methods.
2. a kind of cultural method of anti-grass carp bacterial enteritis disease, comprises the following steps：
(1) improvement of soil：Soil is deep-cut, depth is formed for 1.7m, length is 75m, width is 48m culturing pool, so
Cultivation bottom of pond portion soil block is smashed afterwards, be soaked in water soil 17 days, then according to 40 × 60cm specification centipede is planted on soil
Grass 2.5 years, is removed after ciliate desert-grass, exposure culturing pool 18 days；
(2) management is raised together with：At culturing pool bottom, injecting height is 20cm water, is then planted according to 35 × 45cm specification
Spire iron imperial crown, after spire iron imperial crown grows 34 days, then toward the running water that injecting height in pond is 1.4m, keeps the flowing speed of water
Rate is 850cm3/ min, oral siphon is provided with culturing pool bottom, and outlet pipe is provided with the middle part on oral siphon opposite, in oral siphon and
Outlet pipe respectively installs a water jet oxygen increasing equipment, and water jet oxygenation 7h is carried out daily, 1.1 ten thousand tail fishes are then put in a suitable place to breed in pond
Seedling, the consumption for weighing 4% according to fish body daily feeds grass carp 12 months using feed of grass carp；
(3) sick fish management：When cultivation pool side find it is slow in reacting, peel off solely swim in the water surface, body surface is slight congested or goes out
Blood, belly expand the grass carp red and swollen with anus, pull grass carp out immediately, are soaked with biological disinfectant after sick fish 13min, rinse dry
Only, the isolation tank progress isolation cultivation that with the addition of antiviral agents is then put into, culturing pool can be just returned to after sick fish disease is cured 3 days
Continue to cultivate.
Embodiment 5：
1. early-stage preparations：
The preparation of feed of grass carp：Count by weight, by 50 parts of shepherd's purse leaves, 28 parts of sweet potato leaves and 2.5 parts of mushroom waste mushroom packets
After mixing, sealing and fermenting 33h is mixed into 55 portions of peanut cakes, 38 portions of corn flour, 11 parts of bone meal and 2 parts of garlic powders, is put into height after taking-up
Press in pulser, be that 65kv/cm, umber of pulse are to carry out high-voltage pulse processing 26min under conditions of 280 in electric-field intensity, then
0.35 part of bifidobacterium species atcc liquid and 1 part of bacillus licheniformis kind liquid are mixed into, compression molding after stirring obtains feed of grass carp.
The preparation of biological disinfectant：Count by weight, by 28 parts of staphylococcus lysozymes, 540 parts of epsilon-polylysines, 7 parts
Acetum and 1000 part deionized water mixing of the mass fraction for 1.6%, regulation pH value is 5, obtains biological disinfectant.
The material that above-mentioned early stage is prepared is used for following cultural methods.
2. a kind of cultural method of anti-grass carp bacterial enteritis disease, comprises the following steps：
(1) improvement of soil：Soil is deep-cut, depth is formed for 1.7m, length is 69m, width is 45m culturing pool, so
Cultivation bottom of pond portion soil block is smashed afterwards, be soaked in water soil 19 days, then according to 40 × 60cm specification centipede is planted on soil
Grass 2 years, is removed after ciliate desert-grass, exposure culturing pool 16 days；
(2) management is raised together with：At culturing pool bottom, injecting height is 20cm water, is then planted according to 35 × 45cm specification
Mini green pepper grass, after mini green pepper grass grows 36 days, then toward the running water that injecting height in pond is 1.4m, the flow rate for keeping water is
880cm3/ min, oral siphon is provided with culturing pool bottom, outlet pipe is provided with the middle part on oral siphon opposite, in oral siphon and water outlet
Pipe one water jet oxygen increasing equipment of each installation, carries out water jet oxygenation 6.5h, then puts 1.1 ten thousand tail fries in a suitable place to breed in pond daily,
The consumption for weighing 4.2% according to fish body daily feeds grass carp 12 months using feed of grass carp；
(3) sick fish management：When cultivation pool side find it is slow in reacting, peel off solely swim in the water surface, body surface is slight congested or goes out
Blood, belly expand the grass carp red and swollen with anus, pull grass carp out immediately, are soaked with biological disinfectant after sick fish 12min, rinse dry
Only, the isolation tank progress isolation cultivation that with the addition of antiviral agents is then put into, cultivation can be just returned to after sick fish disease is cured 2.5 days
Continue to cultivate in pond.
Embodiment 6：
1. early-stage preparations：
The preparation of feed of grass carp：Count by weight, 51 parts of shepherd's purse leaves, 40 parts of sweet potato leaves and 3 parts of flat mushroom waste mushroom packets are mixed
After conjunction, sealing and fermenting 36h is mixed into 60 portions of peanut cakes, 40 portions of corn flour, 15 parts of bone meal and 2 parts of garlic powders, is put into high pressure after taking-up
It is that 70kv/cm, umber of pulse are to carry out high-voltage pulse processing 30min under conditions of 300 in electric-field intensity, then mix in pulser
Enter 0.5 part of bifidobacterium species atcc liquid and 1 part of bacillus licheniformis kind liquid, compression molding after stirring obtains feed of grass carp.
The preparation of biological disinfectant：Count by weight, by 33 parts of staphylococcus lysozymes, 600 parts of epsilon-polylysines, 8 parts
Acetum and 1000 part deionized water mixing of the mass fraction for 2%, regulation pH value is 5.2, obtains biological disinfectant.
The material that above-mentioned early stage is prepared is used for following cultural methods.
2. a kind of cultural method of anti-grass carp bacterial enteritis disease, comprises the following steps：
(1) improvement of soil：Soil is deep-cut, depth is formed for 1.8m, length is 80m, width is 50m culturing pool, so
Cultivation bottom of pond portion soil block is smashed afterwards, be soaked in water soil 20 days, then according to 40 × 60cm specification centipede is planted on soil
Grass 3 years, is removed after ciliate desert-grass, exposure culturing pool 20 days；
(2) management is raised together with：At culturing pool bottom, injecting height is 20cm water, is then planted according to 35 × 45cm specification
Small water banyan, after pasture and water grow 40 days, then toward the running water that injecting height in pond is 1.5m, the flow rate for keeping water is
1000cm3/ min, oral siphon is provided with culturing pool bottom, is provided with outlet pipe at the middle part on oral siphon opposite, oral siphon and is being gone out
Water pipe respectively installs a water jet oxygen increasing equipment, and water jet oxygenation 8h is carried out daily, 1.2 ten thousand tail fries are then put in a suitable place to breed in pond,
The consumption for weighing 5% according to fish body daily feeds grass carp 12 months using feed of grass carp；
(3) sick fish management：When cultivation pool side find it is slow in reacting, peel off solely swim in the water surface, body surface is slight congested or goes out
Blood, belly expand the grass carp red and swollen with anus, pull grass carp out immediately, are soaked with biological disinfectant after sick fish 15min, rinse dry
Only, the isolation tank progress isolation cultivation that with the addition of antiviral agents is then put into, culturing pool can be just returned to after sick fish disease is cured 3 days
Continue to cultivate.
Comparative example 1：The specific steps of anti-grass carp bacterial enteritis disease cultural method, raw material are substantially the same manner as Example 1, no
It is with putting：Without plantation ciliate desert-grass in step (1).
Comparative example 2：The specific steps of anti-grass carp bacterial enteritis disease cultural method, raw material are substantially the same manner as Example 1, no
It is with putting：Without plantation pasture and water in step (2).
Comparative example 3：The specific steps of anti-grass carp bacterial enteritis disease cultural method, raw material are substantially the same manner as Example 1, no
It is with putting：Oxygen increasing equipment employed in step (2) is micro-pore aeration oxygen increasing equipment.
Comparative example 4：The specific steps of anti-grass carp bacterial enteritis disease cultural method, raw material are substantially the same manner as Example 1, no
It is with putting：Feed employed in step (2) is the Mount Taishan board Ctenopharyngodon idellua feed sold on the market.
Comparative example 5：The specific steps of anti-grass carp bacterial enteritis disease cultural method, raw material are substantially the same manner as Example 1, no
It is with putting：Disinfectant employed in step (3) is the yellow lid board fish bacterium cleaning/disinfecting agent sold on the market.
Contrast test 1：
Comparative example 1-5 and embodiment 1-6 cultural method is cultivated into grass carp 12 months, detection grass carp is in growth course medium-height grass
The incidence of fish bacterial enteritis disease and the cure rate of bacterial enteritis disease, calculate testing result, the results are shown in Table 1.
Contrast test 2：
Comparative example 1-5 and embodiment 1-6 method are improved to soil, then soil is detected with high performance liquid chromatograph
The heavy metal metal element content such as mercury, lead and chromium, calculates the rate of change of mercury, lead and chromium tenor before and after earth improvement, calculates inspection
Result is surveyed, 1 is the results are shown in Table.
Tenor × 100% before metal rate of change=(tenor after tenor-improvement before improvement)/improvement
Table 1：
The result of table 1 shows：Bacterial enteritis disease incidence is lower, illustrates that the cultural method is more effective.Comparative example 5 it is thin
The incidence highest of bacterium property enteritis disease, the incidence of the bacterial enteritis disease of embodiment 3 is minimum, illustrates by using present invention system
Standby biological disinfectant, can be greatly reduced the incidence of bacterial enteritis disease, reach the effect of prevention bacterial enteritis disease；
Bacterial enteritis disease cure rate is higher, illustrates that the cultural method is more effective.The bacterial enteritis disease of comparative example 5 is controlled
More rate is minimum, the cure rate highest of the bacterial enteritis disease of embodiment 3, illustrates the biological antiseptic prepared by using the present invention
Agent, can be substantially improved the cure rate of bacterial enteritis disease, reach the effect for curing bacterial enteritis disease；
The numerical value of mercury metal rate of change, lead metal rate of change and chromium metal rate of change is higher, illustrate the mercury metal after soil,
The change of lead metal and chromium tenor is maximum, also illustrates that the improved effect of soil is more obvious.Mercury metal, lead in comparative example 1
Metal and chromium metal rate of change are minimum, and the rate of change highest of mercury metal, lead metal and chromium metal in embodiment 3, explanation passes through
Ciliate desert-grass is planted, the purpose of efficient improved soil can be reached.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
The equal change or modification change completed under bright patent claim, the technical spirit suggested by all present invention, all should belong to
Cover the scope of the claims in the present invention.
Claims (6)
1. a kind of cultural method of anti-grass carp bacterial enteritis disease, it is characterised in that：Comprise the following steps：
(1) improvement of soil：Soil is deep-cut, depth is formed for 1.5~1.8m, length is 65~80m, width is 35~50m's
Culturing pool, then smashes cultivation bottom of pond portion soil block, and be soaked in water soil 15~20 days, then according to 40 × 60cm's on soil
Specification plantation ciliate desert-grass 2~3 years, removes after ciliate desert-grass, exposure culturing pool 15~20 days；
(2) management is raised together with：At culturing pool bottom, injecting height is 20cm water, then plants water according to 35 × 45cm specification
Grass, after pasture and water grow 30~40 days, then toward the running water that injecting height in pond is 1.0~1.5m, the flow rate for keeping water is
800~1000cm3/ min, oral siphon is provided with culturing pool bottom, outlet pipe is provided with the middle part on oral siphon opposite, in oral siphon
One water jet oxygen increasing equipment is respectively installed with outlet pipe, water jet 6~8h of oxygenation is carried out daily, then put 0.8 in a suitable place to breed in pond~
1.2 ten thousand tail fries, the consumption for weighing 3~5% according to fish body daily feeds grass carp 12 months using feed of grass carp；
(3) sick fish management：When cultivation pool side find it is slow in reacting, peel off and solely swim in the water surface, body surface be slight congested or bleeding, abdomen
The grass carp red and swollen with anus is expanded in portion, pulls grass carp out immediately, is soaked after 10~15min of sick fish, rinsed well with biological disinfectant,
Then the isolation tank progress isolation cultivation that with the addition of antiviral agents is put into, culturing pool can be just returned to after sick fish disease is cured 2~3 days
Continue to cultivate.
2. a kind of cultural method of anti-grass carp bacterial enteritis disease according to claim 1, it is characterised in that：Step (3)
Described feed of grass carp is prepared by following methods：Count by weight, by 30~51 parts of shepherd's purse leaves, 25~40 parts of sweet potato leaves and 1
After~3 parts of waste mushroom packet mixing, 24~36h of sealing and fermenting is mixed into 30~60 portions of peanut cakes, 20~40 parts of corn flour, 10 after taking-up
~15 parts of bone meal and 2 parts of garlic powders, are put into high-pressure pulse device, are that 35~70kv/cm, umber of pulse are 200 in electric-field intensity
High-voltage pulse is carried out under conditions of~300 and handles 20~30min, 0.3~0.5 part of bifidobacterium species atcc liquid and 1 part of lichens is blended into
Bacillus kind liquid, compression molding after stirring obtains feed of grass carp.
3. a kind of cultural method of anti-grass carp bacterial enteritis disease according to claim 2, it is characterised in that：Described is useless
Bacterium bag is any one in mushroom waste mushroom packet, flat mushroom waste mushroom packet, black fungus waste mushroom packet and asparagus waste mushroom packet.
4. a kind of cultural method of anti-grass carp bacterial enteritis disease according to claim 1, it is characterised in that：Step (2)
Described pasture and water are any one of eel grass, hydrilla verticillata, small najas marina, spire iron imperial crown, the careless and small water banyan of mini green pepper.
5. a kind of cultural method of anti-grass carp bacterial enteritis disease according to claim 1, it is characterised in that：Step (3)
Described biological disinfectant is by being 20~33 by weight：350~600：3~8：1000 staphylococcus lysozyme, ε-poly- bad ammonia
Acid, acetum and deionized water mixing, pH value is 4.5~5.2.
6. a kind of cultural method of anti-grass carp bacterial enteritis disease according to claim 5, it is characterised in that：Described vinegar
The mass fraction of acid solution is 1~2%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108142330A (en) * | 2018-02-11 | 2018-06-12 | 东兰县安篓东二养殖场 | The management method that Hongsuihe River grass carp is fed |
| CN108293922A (en) * | 2018-01-31 | 2018-07-20 | 东兰县安篓东二养殖场 | A kind of cultural method improving selenium-rich grass carp survival rate |
| CN108496701A (en) * | 2018-02-11 | 2018-09-07 | 东兰县安篓东二养殖场 | A kind of high efficiency supports the administrative skill of grass carp |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD236Y (en) * | 2010-03-10 | 2010-07-30 | Institutul De Zoologie Al Academiei De Stiinte A Moldovei | Process for reproduction of grass carp |
| CN102369903A (en) * | 2011-11-26 | 2012-03-14 | 徐伟宁 | Ecological fish tank |
| CN103222561A (en) * | 2013-04-16 | 2013-07-31 | 杨成胜 | Organic grass carp forage and preparation method |
| CN104604759A (en) * | 2014-11-28 | 2015-05-13 | 太和县龙潭鱼牧业养殖有限公司 | Grass carp culturing method |
| CN205727687U (en) * | 2016-06-25 | 2016-11-30 | 四川零关科技有限公司 | A kind of water-spraying oxygen-increasing machine for aquaculture |
| CN106577536A (en) * | 2016-12-20 | 2017-04-26 | 罗城仫佬族自治县金陆地种养农民专业合作社 | Cultivation method for preventing grass carp enteritis disease |
-
2017
- 2017-04-28 CN CN201710296225.XA patent/CN107135994A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD236Y (en) * | 2010-03-10 | 2010-07-30 | Institutul De Zoologie Al Academiei De Stiinte A Moldovei | Process for reproduction of grass carp |
| CN102369903A (en) * | 2011-11-26 | 2012-03-14 | 徐伟宁 | Ecological fish tank |
| CN103222561A (en) * | 2013-04-16 | 2013-07-31 | 杨成胜 | Organic grass carp forage and preparation method |
| CN104604759A (en) * | 2014-11-28 | 2015-05-13 | 太和县龙潭鱼牧业养殖有限公司 | Grass carp culturing method |
| CN205727687U (en) * | 2016-06-25 | 2016-11-30 | 四川零关科技有限公司 | A kind of water-spraying oxygen-increasing machine for aquaculture |
| CN106577536A (en) * | 2016-12-20 | 2017-04-26 | 罗城仫佬族自治县金陆地种养农民专业合作社 | Cultivation method for preventing grass carp enteritis disease |
Non-Patent Citations (6)
| Title |
|---|
| 农业部机械试验鉴定总站等: "《设施水产养殖装备操作工：初级 中级 高级》", 31 May 2014, 中国农业科学技术出版社 * |
| 吕远蓉: "《饲料生产与应用》", 31 October 2015, 西南交通大学出版社 * |
| 廖朝兴等: "《鱼饲料配制与投喂技术160问》", 31 January 2007, 中国农业出版社、农村读物出版社 * |
| 彭世逞等: "《家庭环保花卉大全》", 31 January 2009, 上海科学技术文献出版社 * |
| 杨华明等: "《现代医学消毒学》", 28 February 2013, 人民军医出版社 * |
| 董玉珍等: "《非粮型饲料高效生产技术》", 31 January 2004, 中国农业出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108293922A (en) * | 2018-01-31 | 2018-07-20 | 东兰县安篓东二养殖场 | A kind of cultural method improving selenium-rich grass carp survival rate |
| CN108142330A (en) * | 2018-02-11 | 2018-06-12 | 东兰县安篓东二养殖场 | The management method that Hongsuihe River grass carp is fed |
| CN108496701A (en) * | 2018-02-11 | 2018-09-07 | 东兰县安篓东二养殖场 | A kind of high efficiency supports the administrative skill of grass carp |
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