CN105613386A - Artificial breeding method of icefish fry - Google Patents

Artificial breeding method of icefish fry Download PDF

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Publication number
CN105613386A
CN105613386A CN201610126341.2A CN201610126341A CN105613386A CN 105613386 A CN105613386 A CN 105613386A CN 201610126341 A CN201610126341 A CN 201610126341A CN 105613386 A CN105613386 A CN 105613386A
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days
water
juvenile fish
rearing pool
breed
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刘月月
李传美
刘聪聪
刘升永
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Wuhe County Jinmantang Ecological Plating And Cultivation Home Farm
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Wuhe County Jinmantang Ecological Plating And Cultivation Home Farm
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Fodder In General (AREA)

Abstract

The invention discloses an artificial breeding method of icefish fry, comprising the specific steps: building a pond; selecting parent fishes; spawning induction and inseminating; managing incubation; breeding juvenile fishes; managing in later stage. In the artificial breeding method of icefish fry provided by the invention, the parent fishes are cultured separately and subjected to intensified breeding, maturation accelerating and oxytocin injection, and thus maturation coefficient is increased; artificial insemination is carried out and is simple and safe to operate, a higher quantity of fertilized eggs are provided, central management is facilitated, and manpower and material resources are saved; artificial incubation conditions are under strict control, an incubating pond is sterilized with a plant sterilizer to effectively remove germs and inhibit viruses, with no stimulus to fertilized eggs and no residue, incubating temperature is increased gradually so that adaptability of the fertilized eggs is improved, incubating period is shortened, incubating rate is increased, up to 94.5%, and disease resistance of juvenile fishes is enhanced; before stocking of the juvenile fishes, a juvenile breeding pond is sterilized in a vegetative manner, management is made by stages after hatching, environmental temperature is changed gradually, adaptability of fry is improved, and survival rate is increased, up to 96.3%.

Description

The artificial raise seedling method of a kind of silverfish
Technical field
The present invention relates to technical field of aquaculture, specifically, it relates to the artificial raise seedling method of a kind of silverfish.
Background technology
Silverfish is also called ice fish, glass fish, belongs to silverfish section, fresh-water fishes, and body length is circle slightly, delicate transparent, and color and luster is such as silver, and fine and tender taste, life cycle is short, the generation is discrete, fecundity and to settle down ability strong. In silverfish, protein content is 72.1%, and aminoacids content is also quite abundant, high nutritive value, has the effects such as kidney tonifying increases sun, void of dispelling is invigorated blood circulation, benefit spleen moistening lung, is first-class nourishing tonic. Silverfish is rich in fish calcareous, high protein, lower fat food, does not substantially have big fishbone, and suitable child eats. According to modern nutriology analysis, silverfish is nutritious, has the feature of high protein, lower fat. And think that silverfish does not remove fin, bone, belong to " globality food (namely internal organ, head, wing all do not remove; overall edible) ", comprehensive nutrition, it is beneficial to human body and promotes immunologic function, promote longevity, especially it is suitable for physique weakness, under-nutrition, maldigestion person, hyperlipidemia patient, weakness of the spleen and the stomach person, have the edible for patients such as cough due to deficiency of the lung, empty labor. Silverfish is as a kind of globality food, and the function that its health lengthens one's life is confirmed for world nutrition educational circles.
Silverfish is one of Chinese famous and precious fishery products, is regarded as on seat treasure, at home and abroad has long enjoyed a good reputation. But in present silverfish breeding process, due to the shortcoming of water body, bait and management aspect so that run into all difficulties in silverfish breeding process, serious impact cultivates the income of peasant household.
Summary of the invention
In order to make up the defect of prior art, it is an object of the invention to provide the artificial raise seedling method of a kind of silverfish.
An artificial raise seedling method for silverfish, concrete comprises the following steps:
(1) pond is set up: select leeward on the sunny side, water body is stable, water quality is pure and fresh, traffic easily environment set up parent population rearing pool and juvenile fish rearing pool, parent population rearing pool length 50 ~ 55m, wide 20 ~ 25m, dark 2.5 ~ 2.7m, the depth of water 1.8 ~ 2.0m; Juvenile fish rearing pool length 90 ~ 100m, wide 45 ~ 50m, dark 3.2 ~ 3.5m, the depth of water 1.8 ~ 2.2m;
(2) parent population is selected: within 20 ~ 25 days before the mating period of silverfish, fish for physique stalwartness, male and female silverfish anosis, in good condition as parent population, female: hero=2:5, by male and female silverfish after the salt solution that mass concentration is 0.6% soaks 4 ~ 6 minutes, put in a suitable place to breed respectively in different rearing pool, put first 13 ~ 15 days in a suitable place to breed, parent population rearing pool need to be cleaned up the pond, and carries out pasteurised completely, spills dry lime 20 ~ 25g/m2, after rinsing well, add river degerming after filtration, put first 9 ~ 10 days in a suitable place to breed, cultivate parent population bait, put first 4 ~ 5 days in a suitable place to breed, evenly add EM bacterium 3 ~ 4g/m3, increasing probiotics, suppress the growth of pathogenic micro-organism, control water temperature is 15 ~ 16 DEG C, and dissolved oxygen amount is 6 ~ 8mg/L, strengthening raising 12 ~ 14 days;
(3) hasten parturition insemination: to parent population oxytocin injection, the first pin injection: chorionic-gonadotropin hormone (HCG) 140 ~ 160IU/kg, luteotropin releasing hormone d-ala analog (LRH-A) 3 ~ 4mg/Kg; 2nd pin injection: HCG80 ~ 100IU/kg, LRH-A1 ~ 2mg/Kg, injection volume calculates with raun body weight, milter reduces by half, every two pin interval times are 7 ~ 8 hours, 2nd latter 6 ~ 8 hours of pin injection, takes milter seminal vesicle, obtains seminal fluid, squeeze the ovum getting raun, mixing with seminal fluid, the salt solution being 0.6% by the mass concentration with seminal fluid equivalent adds in mixed solution, dilutes, stir evenly gently, leave standstill 20 ~ 30 minutes, then stir gently evenly, continue fertilization 3 ~ 4 hours, clean with physiological saline, remove impurity;
(4) hatching management: zygote taken out, after sterilizing with sterilizing agent, is placed in indoor brooder, room temp and water temperature is kept to be 5 ~ 7 DEG C, change water every day 2 times, the time be morning 7:00 ~ 8:00 and afternoon 5:00 ~ 6:00, quantity of exchanged water is 100%, every day observes, before membrane 8 ~ 9 days, it is to increase room temp and water temperature to 12 ~ 14 DEG C, before membrane 2 ~ 3 days, make room temp and water temperature to 15 ~ 16 DEG C, to incubating oosperm membrane;
(5) juvenile fish is raised: juvenile fish puts first 15 ~ 16 days in a suitable place to breed, and the salt solution being 12 ~ 14% by mass concentration scrubs juvenile fish rearing pool, after moisture spontaneous evaporation, rinses well with clear water, then spills dry lime 17 ~ 19g/m2Sterilize, rinse well with clear water again, put first 7 ~ 8 days in a suitable place to breed, inject Chi Shui, plantation water peanut and lotus of wandering without a fixed dwelling, area coverage is the 3/5 of rearing pool area, water temperature is kept to be 26 ~ 28 DEG C, cultivate juvenile fish bait, put first 3 ~ 4 days in a suitable place to breed, the water temperature of juvenile fish rearing pool is down to 7 ~ 8 DEG C, add the sterilizing agent that step (4) is used, after juvenile fish membrane 1 ~ 4 day, it is placed in the clear water that temperature is 7 ~ 8 DEG C, within every 2 days, change water once, juvenile fish was put in a suitable place to breed in juvenile fish rearing pool in 5th day, adapt to the environment of rearing pool, being beneficial to the later stage looks for food, after juvenile fish starts to look for food, the bait amount keeping juvenile fish rearing pool is 6.4 ~ 6.6mg/L, it is 25th ~ 30 after putting in a suitable place to breed day that rearing pool changes water for the 1st time, within every 7 days later, change water 1 time, quantity of exchanged water is the 1/4 of former Chi Shui,
(6) final-period management: after juvenile fish membrane 30 ~ 40 days, keep juvenile fish rearing pool water temperature to be 10 ~ 11 DEG C, after membrane 41 ~ 50 days, keep juvenile fish rearing pool water temperature to be 12 ~ 14 DEG C, within after membrane 51 days, put seedling in a suitable place to breed big pond in water temperature is 15 ~ 17 DEG C and raise, or sell.
The sterilizing agent of described step (4), it is made up of the plant disinfectant of following weight part: reach the clouds 62 ~ 64, China tree 62 ~ 64, hang down Chinese scholartree leaf 61 ~ 63, Seed of Chinese Soapberry 57 ~ 59, Chinese pistache leaf 57 ~ 59, bean curd leaf 52 ~ 54, Chinese wistaria spends 51 ~ 53, eucalyptus leaves 51 ~ 53, peach leaves 42 ~ 44, Cortex Ulmi Parvifoliae 37 ~ 39, Scammonia resin 32 ~ 34, all plant materials are pulverized, cross 100 ~ 120 order sieves, add the water of all plant material gross weight 3 ~ 4 times amount, it is heated to boiling, little fire is endured and is boiled 50 ~ 60 minutes, filter to obtain plant extraction liquid and plant slag, the volume fraction adding plant slag gross weight 3 ~ 4 times amount in plant slag is the spirituous solution of 51 ~ 53%, it is heated to 82 ~ 84 DEG C, ultrasonic 42 ~ 44 minutes, centrifugal, remove plant slag, merge all extracting solutions, concentrate to gross weight be 3 times of plant material gross weight, obtain plant disinfectant, during use, plant disinfectant is added to the water, add-on is 6 ~ 7g/L, mix, can use.
It is an advantage of the invention that: the artificial raise seedling method of a kind of silverfish provided by the invention, the parent population of insufficient maturation is carried out separately cultivation, strengthening is raised, promote ripe, strengthen reproduction ability, point two pin oxytocin injections, manually hasten parturition, dosage is suitable for, and makes the parent population ripening stage in advance, significantly improves parent population mature coefficient; Carrying out artificial insemination, simple to operate, safety, obtain the whole seminal fluid of milter, the number ratio maximizing of male and female parent population utilizes seminal fluid, obviously increases zygote quantity, fertilization is concentrated, fast, is convenient to centralized management, save manpower and materials; Strict control condition carries out artificial incubation, sterilize through plant disinfectant in hatchery, effectively degerming, suppresses virus, gentle, non-stimulated, the noresidue to zygote, improving incubation temperature gradually, high temperature in short-term can stimulate the resistance of zygote, it is possible to improves the adaptive faculty of zygote, shorten the incubation period, improve hatching rate, reach 94.5%, strengthen the resistance against diseases of the rear juvenile fish of hatching; Before juvenile fish is put in a suitable place to breed, juvenile fish rearing pool is carried out plant sterilization, high-temperature cultivation bait, shorten incubation time, manage after membrane, Gradual scene change temperature, it is to increase seedling, to the adaptability of environment, strengthens resistance against diseases by stages, improve surviving rate, reach 96.3%, it is to increase income.
Embodiment
With specific embodiment, the present invention is described below, but it is not limitation of the present invention.
Embodiment 1
An artificial raise seedling method for silverfish, concrete comprises the following steps:
(1) pond is set up: select leeward on the sunny side, water body is stable, water quality is pure and fresh, traffic easily environment set up parent population rearing pool and juvenile fish rearing pool, parent population rearing pool length 50 ~ 55m, wide 20 ~ 25m, dark 2.5 ~ 2.7m, the depth of water 1.8 ~ 2.0m; Juvenile fish rearing pool length 90 ~ 100m, wide 45 ~ 50m, dark 3.2 ~ 3.5m, the depth of water 1.8 ~ 2.2m;
(2) parent population is selected: within 20 ~ 25 days before the mating period of silverfish, fish for physique stalwartness, male and female silverfish anosis, in good condition as parent population, female: hero=2:5, by male and female silverfish after the salt solution that mass concentration is 0.6% soaks 4 ~ 6 minutes, put in a suitable place to breed respectively in different rearing pool, put first 13 ~ 15 days in a suitable place to breed, parent population rearing pool need to be cleaned up the pond, and carries out pasteurised completely, spills dry lime 20g/m2, after rinsing well, add river degerming after filtration, put first 9 ~ 10 days in a suitable place to breed, cultivate parent population bait, put first 4 ~ 5 days in a suitable place to breed, evenly add EM bacterium 3g/m3, increasing probiotics, suppress the growth of pathogenic micro-organism, control water temperature is 15 ~ 16 DEG C, and dissolved oxygen amount is 6mg/L, strengthening raising 12 ~ 14 days;
(3) hasten parturition insemination: to parent population oxytocin injection, the first pin injection: chorionic-gonadotropin hormone (HCG) 140IU/kg, luteotropin releasing hormone d-ala analog (LRH-A) 3mg/Kg; 2nd pin injection: HCG80IU/kg, LRH-A1mg/Kg, injection volume calculates with raun body weight, milter reduces by half, every two pin interval times are 7 ~ 8 hours, 2nd latter 6 ~ 8 hours of pin injection, takes milter seminal vesicle, obtains seminal fluid, squeeze the ovum getting raun, mixing with seminal fluid, the salt solution being 0.6% by the mass concentration with seminal fluid equivalent adds in mixed solution, dilutes, stir evenly gently, leave standstill 20 minutes, then stir gently evenly, continue fertilization 3 ~ 4 hours, clean with physiological saline, remove impurity;
(4) hatching management: zygote taken out, after sterilizing with sterilizing agent, is placed in indoor brooder, room temp and water temperature is kept to be 5 ~ 7 DEG C, change water every day 2 times, the time be morning 7:00 ~ 8:00 and afternoon 5:00 ~ 6:00, quantity of exchanged water is 100%, every day observes, before membrane 8 ~ 9 days, it is to increase room temp and water temperature to 12 ~ 14 DEG C, before membrane 2 ~ 3 days, make room temp and water temperature to 15 ~ 16 DEG C, to incubating oosperm membrane;
(5) juvenile fish is raised: juvenile fish puts first 15 ~ 16 days in a suitable place to breed, and the salt solution being 12 ~ 14% by mass concentration scrubs juvenile fish rearing pool, after moisture spontaneous evaporation, rinses well with clear water, then spills dry lime 17g/m2Sterilize, rinse well with clear water again, put first 7 ~ 8 days in a suitable place to breed, inject Chi Shui, plantation water peanut and lotus of wandering without a fixed dwelling, area coverage is the 3/5 of rearing pool area, water temperature is kept to be 26 ~ 28 DEG C, cultivate juvenile fish bait, put first 3 ~ 4 days in a suitable place to breed, the water temperature of juvenile fish rearing pool is down to 7 ~ 8 DEG C, add the sterilizing agent that step (4) is used, after juvenile fish membrane 1 ~ 4 day, it is placed in the clear water that temperature is 7 ~ 8 DEG C, within every 2 days, change water once, juvenile fish was put in a suitable place to breed in juvenile fish rearing pool in 5th day, adapt to the environment of rearing pool, being beneficial to the later stage looks for food, after juvenile fish starts to look for food, the bait amount keeping juvenile fish rearing pool is 6.4mg/L, it is 25th ~ 30 after putting in a suitable place to breed day that rearing pool changes water for the 1st time, within every 7 days later, change water 1 time, quantity of exchanged water is the 1/4 of former Chi Shui,
(6) final-period management: after juvenile fish membrane 30 ~ 40 days, keep juvenile fish rearing pool water temperature to be 10 ~ 11 DEG C, after membrane 41 ~ 50 days, keep juvenile fish rearing pool water temperature to be 12 ~ 14 DEG C, within after membrane 51 days, put seedling in a suitable place to breed big pond in water temperature is 15 ~ 17 DEG C and raise, or sell.
The sterilizing agent of described step (4), it is made up of the plant disinfectant of following weight part: reach the clouds 62, China tree 62, hang down Chinese scholartree leaf 61, Seed of Chinese Soapberry 57, Chinese pistache leaf 57, bean curd leaf 52, Chinese wistaria spends 51, eucalyptus leaves 51, peach leaves 42, Cortex Ulmi Parvifoliae 37, Scammonia resin 32, all plant materials are pulverized, cross 100 order sieves, add the water of all plant material gross weight 3 times amount, it is heated to boiling, little fire is endured and is boiled 50 minutes, filter to obtain plant extraction liquid and plant slag, the volume fraction adding plant slag gross weight 3 times amount in plant slag is the spirituous solution of 51%, it is heated to 82 ~ 84 DEG C, ultrasonic 42 minutes, centrifugal, remove plant slag, merge all extracting solutions, concentrate to gross weight be 3 times of plant material gross weight, obtain plant disinfectant, during use, plant disinfectant is added to the water, add-on is 6g/L, mix, can use.
Embodiment 2
An artificial raise seedling method for silverfish, concrete comprises the following steps:
(1) pond is set up: select leeward on the sunny side, water body is stable, water quality is pure and fresh, traffic easily environment set up parent population rearing pool and juvenile fish rearing pool, parent population rearing pool length 50 ~ 55m, wide 20 ~ 25m, dark 2.5 ~ 2.7m, the depth of water 1.8 ~ 2.0m; Juvenile fish rearing pool length 90 ~ 100m, wide 45 ~ 50m, dark 3.2 ~ 3.5m, the depth of water 1.8 ~ 2.2m;
(2) parent population is selected: within 20 ~ 25 days before the mating period of silverfish, fish for physique stalwartness, male and female silverfish anosis, in good condition as parent population, female: hero=2:5, by male and female silverfish after the salt solution that mass concentration is 0.6% soaks 4 ~ 6 minutes, put in a suitable place to breed respectively in different rearing pool, put first 13 ~ 15 days in a suitable place to breed, parent population rearing pool need to be cleaned up the pond, and carries out pasteurised completely, spills dry lime 23g/m2, after rinsing well, add river degerming after filtration, put first 9 ~ 10 days in a suitable place to breed, cultivate parent population bait, put first 4 ~ 5 days in a suitable place to breed, evenly add EM bacterium 3.5g/m3, increasing probiotics, suppress the growth of pathogenic micro-organism, control water temperature is 15 ~ 16 DEG C, and dissolved oxygen amount is 7mg/L, strengthening raising 12 ~ 14 days;
(3) hasten parturition insemination: to parent population oxytocin injection, the first pin injection: chorionic-gonadotropin hormone (HCG) 150IU/kg, luteotropin releasing hormone d-ala analog (LRH-A) 3.5mg/Kg; 2nd pin injection: HCG90IU/kg, LRH-A1.5mg/Kg, injection volume calculates with raun body weight, milter reduces by half, every two pin interval times are 7 ~ 8 hours, 2nd latter 6 ~ 8 hours of pin injection, takes milter seminal vesicle, obtains seminal fluid, squeeze the ovum getting raun, mixing with seminal fluid, the salt solution being 0.6% by the mass concentration with seminal fluid equivalent adds in mixed solution, dilutes, stir evenly gently, leave standstill 25 minutes, then stir gently evenly, continue fertilization 3 ~ 4 hours, clean with physiological saline, remove impurity;
(4) hatching management: zygote taken out, after sterilizing with sterilizing agent, is placed in indoor brooder, room temp and water temperature is kept to be 5 ~ 7 DEG C, change water every day 2 times, the time be morning 7:00 ~ 8:00 and afternoon 5:00 ~ 6:00, quantity of exchanged water is 100%, every day observes, before membrane 8 ~ 9 days, it is to increase room temp and water temperature to 12 ~ 14 DEG C, before membrane 2 ~ 3 days, make room temp and water temperature to 15 ~ 16 DEG C, to incubating oosperm membrane;
(5) juvenile fish is raised: juvenile fish puts first 15 ~ 16 days in a suitable place to breed, and the salt solution being 13% by mass concentration scrubs juvenile fish rearing pool, after moisture spontaneous evaporation, rinses well with clear water, then spills dry lime 18g/m2Sterilize, rinse well with clear water again, put first 7 ~ 8 days in a suitable place to breed, inject Chi Shui, plantation water peanut and lotus of wandering without a fixed dwelling, area coverage is the 3/5 of rearing pool area, water temperature is kept to be 26 ~ 28 DEG C, cultivate juvenile fish bait, put first 3 ~ 4 days in a suitable place to breed, the water temperature of juvenile fish rearing pool is down to 7 ~ 8 DEG C, add the sterilizing agent that step (4) is used, after juvenile fish membrane 1 ~ 4 day, it is placed in the clear water that temperature is 7 ~ 8 DEG C, within every 2 days, change water once, juvenile fish was put in a suitable place to breed in juvenile fish rearing pool in 5th day, adapt to the environment of rearing pool, being beneficial to the later stage looks for food, after juvenile fish starts to look for food, the bait amount keeping juvenile fish rearing pool is 6.5mg/L, it is 25th ~ 30 after putting in a suitable place to breed day that rearing pool changes water for the 1st time, within every 7 days later, change water 1 time, quantity of exchanged water is the 1/4 of former Chi Shui,
(6) final-period management: after juvenile fish membrane 30 ~ 40 days, keep juvenile fish rearing pool water temperature to be 10 ~ 11 DEG C, after membrane 41 ~ 50 days, keep juvenile fish rearing pool water temperature to be 12 ~ 14 DEG C, within after membrane 51 days, put seedling in a suitable place to breed big pond in water temperature is 15 ~ 17 DEG C and raise, or sell.
The sterilizing agent of described step (4), it is made up of the plant disinfectant of following weight part: reach the clouds 63, China tree 63, hang down Chinese scholartree leaf 62, Seed of Chinese Soapberry 58, Chinese pistache leaf 58, bean curd leaf 53, Chinese wistaria spends 52, eucalyptus leaves 52, peach leaves 43, Cortex Ulmi Parvifoliae 38, Scammonia resin 33, all plant materials are pulverized, cross 100 order sieves, add the water of all plant material gross weight 3 times amount, it is heated to boiling, little fire is endured and is boiled 55 minutes, filter to obtain plant extraction liquid and plant slag, the volume fraction adding plant slag gross weight 3 times amount in plant slag is the spirituous solution of 52%, it is heated to 82 ~ 84 DEG C, ultrasonic 43 minutes, centrifugal, remove plant slag, merge all extracting solutions, concentrate to gross weight be 3 times of plant material gross weight, obtain plant disinfectant, during use, plant disinfectant is added to the water, add-on is 6.5g/L, mix, can use.
Embodiment 3
An artificial raise seedling method for silverfish, concrete comprises the following steps:
(1) pond is set up: select leeward on the sunny side, water body is stable, water quality is pure and fresh, traffic easily environment set up parent population rearing pool and juvenile fish rearing pool, parent population rearing pool length 50 ~ 55m, wide 20 ~ 25m, dark 2.5 ~ 2.7m, the depth of water 1.8 ~ 2.0m; Juvenile fish rearing pool length 90 ~ 100m, wide 45 ~ 50m, dark 3.2 ~ 3.5m, the depth of water 1.8 ~ 2.2m;
(2) parent population is selected: within 20 ~ 25 days before the mating period of silverfish, fish for physique stalwartness, male and female silverfish anosis, in good condition as parent population, female: hero=2:5, by male and female silverfish after the salt solution that mass concentration is 0.6% soaks 4 ~ 6 minutes, put in a suitable place to breed respectively in different rearing pool, put first 13 ~ 15 days in a suitable place to breed, parent population rearing pool need to be cleaned up the pond, and carries out pasteurised completely, spills dry lime 25g/m2, after rinsing well, add river degerming after filtration, put first 9 ~ 10 days in a suitable place to breed, cultivate parent population bait, put first 4 ~ 5 days in a suitable place to breed, evenly add EM bacterium 4g/m3, increasing probiotics, suppress the growth of pathogenic micro-organism, control water temperature is 15 ~ 16 DEG C, and dissolved oxygen amount is 8mg/L, strengthening raising 12 ~ 14 days;
(3) hasten parturition insemination: to parent population oxytocin injection, the first pin injection: chorionic-gonadotropin hormone (HCG) 160IU/kg, luteotropin releasing hormone d-ala analog (LRH-A) 4mg/Kg; 2nd pin injection: HCG100IU/kg, LRH-A2mg/Kg, injection volume calculates with raun body weight, milter reduces by half, every two pin interval times are 7 ~ 8 hours, 2nd latter 6 ~ 8 hours of pin injection, takes milter seminal vesicle, obtains seminal fluid, squeeze the ovum getting raun, mixing with seminal fluid, the salt solution being 0.6% by the mass concentration with seminal fluid equivalent adds in mixed solution, dilutes, stir evenly gently, leave standstill 30 minutes, then stir gently evenly, continue fertilization 3 ~ 4 hours, clean with physiological saline, remove impurity;
(4) hatching management: zygote taken out, after sterilizing with sterilizing agent, is placed in indoor brooder, room temp and water temperature is kept to be 5 ~ 7 DEG C, change water every day 2 times, the time be morning 7:00 ~ 8:00 and afternoon 5:00 ~ 6:00, quantity of exchanged water is 100%, every day observes, before membrane 8 ~ 9 days, it is to increase room temp and water temperature to 12 ~ 14 DEG C, before membrane 2 ~ 3 days, make room temp and water temperature to 15 ~ 16 DEG C, to incubating oosperm membrane;
(5) juvenile fish is raised: juvenile fish puts first 15 ~ 16 days in a suitable place to breed, and the salt solution being 14% by mass concentration scrubs juvenile fish rearing pool, after moisture spontaneous evaporation, rinses well with clear water, then spills dry lime 19g/m2Sterilize, rinse well with clear water again, put first 7 ~ 8 days in a suitable place to breed, inject Chi Shui, plantation water peanut and lotus of wandering without a fixed dwelling, area coverage is the 3/5 of rearing pool area, water temperature is kept to be 26 ~ 28 DEG C, cultivate juvenile fish bait, put first 3 ~ 4 days in a suitable place to breed, the water temperature of juvenile fish rearing pool is down to 7 ~ 8 DEG C, add the sterilizing agent that step (4) is used, after juvenile fish membrane 1 ~ 4 day, it is placed in the clear water that temperature is 7 ~ 8 DEG C, within every 2 days, change water once, juvenile fish was put in a suitable place to breed in juvenile fish rearing pool in 5th day, adapt to the environment of rearing pool, being beneficial to the later stage looks for food, after juvenile fish starts to look for food, the bait amount keeping juvenile fish rearing pool is 6.6mg/L, it is 25th ~ 30 after putting in a suitable place to breed day that rearing pool changes water for the 1st time, within every 7 days later, change water 1 time, quantity of exchanged water is the 1/4 of former Chi Shui,
(6) final-period management: after juvenile fish membrane 30 ~ 40 days, keep juvenile fish rearing pool water temperature to be 10 ~ 11 DEG C, after membrane 41 ~ 50 days, keep juvenile fish rearing pool water temperature to be 12 ~ 14 DEG C, within after membrane 51 days, put seedling in a suitable place to breed big pond in water temperature is 15 ~ 17 DEG C and raise, or sell.
The sterilizing agent of described step (4), it is made up of the plant disinfectant of following weight part: reach the clouds 64, China tree 64, hang down Chinese scholartree leaf 63, Seed of Chinese Soapberry 59, Chinese pistache leaf 59, bean curd leaf 54, Chinese wistaria spends 53, eucalyptus leaves 53, peach leaves 44, Cortex Ulmi Parvifoliae 39, Scammonia resin 34, all plant materials are pulverized, cross 120 order sieves, add the water of all plant material gross weight 4 times amount, it is heated to boiling, little fire is endured and is boiled 60 minutes, filter to obtain plant extraction liquid and plant slag, the volume fraction adding plant slag gross weight 4 times amount in plant slag is the spirituous solution of 53%, it is heated to 82 ~ 84 DEG C, ultrasonic 44 minutes, centrifugal, remove plant slag, merge all extracting solutions, concentrate to gross weight be 3 times of plant material gross weight, obtain plant disinfectant, during use, plant disinfectant is added to the water, add-on is 7g/L, mix, can use.
Comparative example
Conventional silverfish method for culturing seedlings.
The method for culturing seedlings of embodiment and comparative example silverfish is on the impact of seedling:
According to the method for culturing seedlings of embodiment and the silverfish of comparative example, in identical area, cultivating identical silverfish kind, identical parent population quantity, the impact of seedling be the results are shown in Table 1 by the method for culturing seedlings of embodiment and comparative example silverfish.
Table 1: the method for culturing seedlings of embodiment and comparative example silverfish is on the impact of seedling
Project Embodiment 1 Embodiment 2 Embodiment 3 Comparative example
Mature coefficient/(%) 72.2 73.1 72.7 39.3
Zygote number/(individual) 7236 7132 7311 5836
Rate of fertilization/(%) 85.7 86.3 86.6 70.4
Hatching rate/(%) 93.6 94.5 94.2 78.3
Surviving rate/(%) 96.1 96.3 95.8 75.7
Year mu benefit/(ten thousand yuan) 9.4 10.6 10.3 8.2
From the results shown in Table 1, the artificial raise seedling method of embodiment silverfish, in silverfish seedling raising process, mature coefficient, rate of fertilization, hatching rate, surviving rate are obviously higher than comparative example, zygote number and year mu benefit relatively comparative example height, illustrate that the artificial raise seedling method implementation of silverfish provided by the invention is good, high financial profit.

Claims (2)

1. the artificial raise seedling method of a silverfish, it is characterised in that, concrete comprises the following steps:
(1) pond is set up: select leeward on the sunny side, water body is stable, water quality is pure and fresh, traffic easily environment set up parent population rearing pool and juvenile fish rearing pool, parent population rearing pool length 50 ~ 55m, wide 20 ~ 25m, dark 2.5 ~ 2.7m, the depth of water 1.8 ~ 2.0m; Juvenile fish rearing pool length 90 ~ 100m, wide 45 ~ 50m, dark 3.2 ~ 3.5m, the depth of water 1.8 ~ 2.2m;
(2) parent population is selected: within 20 ~ 25 days before the mating period of silverfish, fish for physique stalwartness, male and female silverfish anosis, in good condition as parent population, female: hero=2:5, by male and female silverfish after the salt solution that mass concentration is 0.6% soaks 4 ~ 6 minutes, put in a suitable place to breed respectively in different rearing pool, put first 13 ~ 15 days in a suitable place to breed, parent population rearing pool need to be cleaned up the pond, and spills dry lime 20 ~ 25g/m2, after rinsing well, add river degerming after filtration, put first 9 ~ 10 days in a suitable place to breed, cultivate parent population bait, put first 4 ~ 5 days in a suitable place to breed, evenly add EM bacterium 3 ~ 4g/m3, control water temperature is 15 ~ 16 DEG C, and dissolved oxygen amount is 6 ~ 8mg/L, strengthening raising 12 ~ 14 days;
(3) hasten parturition insemination: to parent population oxytocin injection, the first pin injection: chorionic-gonadotropin hormone (HCG) 140 ~ 160IU/kg, luteotropin releasing hormone d-ala analog (LRH-A) 3 ~ 4mg/Kg; 2nd pin injection: HCG80 ~ 100IU/kg, LRH-A1 ~ 2mg/Kg, injection volume calculates with raun body weight, and milter reduces by half, every two pin interval times are 7 ~ 8 hours, after the 2nd pin injection 6 ~ 8 hours, take milter seminal vesicle, obtain seminal fluid, squeeze the ovum getting raun, mix with seminal fluid, the salt solution being 0.6% by the mass concentration with seminal fluid equivalent adds in mixed solution, stir gently evenly, leave standstill 20 ~ 30 minutes, then stir evenly gently, continue fertilization 3 ~ 4 hours, clean with physiological saline;
(4) hatching management: zygote taken out, after sterilizing with sterilizing agent, is placed in indoor brooder, room temp and water temperature is kept to be 5 ~ 7 DEG C, change water every day 2 times, the time be morning 7:00 ~ 8:00 and afternoon 5:00 ~ 6:00, quantity of exchanged water is 100%, every day observes, before membrane 8 ~ 9 days, it is to increase room temp and water temperature to 12 ~ 14 DEG C, before membrane 2 ~ 3 days, make room temp and water temperature to 15 ~ 16 DEG C, to incubating oosperm membrane;
(5) juvenile fish is raised: juvenile fish puts first 15 ~ 16 days in a suitable place to breed, the salt solution being 12 ~ 14% by mass concentration scrubs juvenile fish rearing pool, after moisture spontaneous evaporation, rinse well with clear water, spill dry lime 17 ~ 19g/m2 again to sterilize, rinse well with clear water again, put first 7 ~ 8 days in a suitable place to breed, inject Chi Shui, plantation water peanut and lotus of wandering without a fixed dwelling, area coverage is the 3/5 of rearing pool area, water temperature is kept to be 26 ~ 28 DEG C, cultivate juvenile fish bait, put first 3 ~ 4 days in a suitable place to breed, the water temperature of juvenile fish rearing pool is down to 7 ~ 8 DEG C, add the sterilizing agent that step (4) is used, after juvenile fish membrane 1 ~ 4 day, it is placed in the clear water that temperature is 7 ~ 8 DEG C, within every 2 days, change water once, juvenile fish was put in a suitable place to breed in juvenile fish rearing pool in 5th day, after juvenile fish starts to look for food, the bait amount keeping juvenile fish rearing pool is 6.4 ~ 6.6mg/L, it is 25th ~ 30 after putting in a suitable place to breed day that rearing pool changes water for the 1st time, within every 7 days later, change water 1 time, quantity of exchanged water is the 1/4 of former Chi Shui,
(6) final-period management: after juvenile fish membrane 30 ~ 40 days, keep juvenile fish rearing pool water temperature to be 10 ~ 11 DEG C, after membrane 41 ~ 50 days, keep juvenile fish rearing pool water temperature to be 12 ~ 14 DEG C, within after membrane 51 days, put seedling in a suitable place to breed big pond in water temperature is 15 ~ 17 DEG C and raise, or sell.
2. the artificial raise seedling method of silverfish according to claim 1, it is characterized in that, the sterilizing agent of described step (4), it is made up of the plant disinfectant of following weight part: reach the clouds 62 ~ 64, China tree 62 ~ 64, hang down Chinese scholartree leaf 61 ~ 63, Seed of Chinese Soapberry 57 ~ 59, Chinese pistache leaf 57 ~ 59, bean curd leaf 52 ~ 54, Chinese wistaria spends 51 ~ 53, eucalyptus leaves 51 ~ 53, peach leaves 42 ~ 44, Cortex Ulmi Parvifoliae 37 ~ 39, Scammonia resin 32 ~ 34, all plant materials are pulverized, cross 100 ~ 120 order sieves, add the water of all plant material gross weight 3 ~ 4 times amount, it is heated to boiling, little fire is endured and is boiled 50 ~ 60 minutes, filter to obtain plant extraction liquid and plant slag, the volume fraction adding plant slag gross weight 3 ~ 4 times amount in plant slag is the spirituous solution of 51 ~ 53%, it is heated to 82 ~ 84 DEG C, ultrasonic 42 ~ 44 minutes, centrifugal, remove plant slag, merge all extracting solutions, concentrate to gross weight be 3 times of plant material gross weight, obtain plant disinfectant, during use, plant disinfectant is added to the water, add-on is 6 ~ 7g/L, mix, can use.
CN201610126341.2A 2016-03-07 2016-03-07 Artificial breeding method of icefish fry Pending CN105613386A (en)

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