CN106212333A - A kind of artificial fecundation method of north palpus loach - Google Patents
A kind of artificial fecundation method of north palpus loach Download PDFInfo
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- CN106212333A CN106212333A CN201610609039.2A CN201610609039A CN106212333A CN 106212333 A CN106212333 A CN 106212333A CN 201610609039 A CN201610609039 A CN 201610609039A CN 106212333 A CN106212333 A CN 106212333A
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- 241000252185 Cobitidae Species 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 25
- 241000251468 Actinopterygii Species 0.000 claims abstract description 120
- 230000002196 ecbolic effect Effects 0.000 claims abstract description 27
- 210000004602 germ cell Anatomy 0.000 claims abstract description 17
- 238000002156 mixing Methods 0.000 claims abstract description 17
- 230000032696 parturition Effects 0.000 claims abstract description 17
- 239000004568 cement Substances 0.000 claims abstract description 16
- 239000003814 drug Substances 0.000 claims abstract description 10
- 229940079593 drug Drugs 0.000 claims abstract description 10
- 239000008239 natural water Substances 0.000 claims abstract description 10
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims abstract description 9
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims abstract description 9
- 229940015047 chorionic gonadotropin Drugs 0.000 claims abstract description 9
- 230000012447 hatching Effects 0.000 claims abstract description 9
- 239000005556 hormone Substances 0.000 claims abstract description 9
- 229940088597 hormone Drugs 0.000 claims abstract description 9
- 230000009027 insemination Effects 0.000 claims abstract description 9
- 210000003205 muscle Anatomy 0.000 claims abstract description 9
- 238000002360 preparation method Methods 0.000 claims abstract description 9
- 108700012941 GNRH1 Proteins 0.000 claims abstract description 8
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 84
- 102000002322 Egg Proteins Human genes 0.000 claims description 25
- 108010000912 Egg Proteins Proteins 0.000 claims description 25
- 210000004681 ovum Anatomy 0.000 claims description 25
- 230000037396 body weight Effects 0.000 claims description 24
- 238000003860 storage Methods 0.000 claims description 16
- 230000003203 everyday effect Effects 0.000 claims description 13
- 238000011534 incubation Methods 0.000 claims description 10
- 238000001125 extrusion Methods 0.000 claims description 9
- 241000361919 Metaphire sieboldi Species 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 230000003187 abdominal effect Effects 0.000 claims description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 8
- 238000007598 dipping method Methods 0.000 claims description 8
- 238000002224 dissection Methods 0.000 claims description 8
- 230000036541 health Effects 0.000 claims description 8
- 238000002347 injection Methods 0.000 claims description 8
- 239000007924 injection Substances 0.000 claims description 8
- 229910052760 oxygen Inorganic materials 0.000 claims description 8
- 239000001301 oxygen Substances 0.000 claims description 8
- 210000000582 semen Anatomy 0.000 claims description 8
- 239000000919 ceramic Substances 0.000 claims description 7
- 239000008188 pellet Substances 0.000 claims description 7
- 210000001672 ovary Anatomy 0.000 claims description 6
- 230000003287 optical effect Effects 0.000 claims 3
- 235000013601 eggs Nutrition 0.000 abstract description 5
- 230000004720 fertilization Effects 0.000 abstract description 3
- 230000002710 gonadal effect Effects 0.000 abstract description 2
- 230000035800 maturation Effects 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000252206 Cypriniformes Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000487097 Nemacheilidae Species 0.000 description 1
- 241000998584 Nuda Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000002611 ovarian Effects 0.000 description 1
- 230000016087 ovulation Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The artificial fecundation method of a kind of north palpus loach, comprises the steps: one, gathers kind of a fish: fish from natural water area and select healthy wild fish, start to collect palpus loach parent fish from the north at the beginning of 3 months.Two, put in a suitable place to breed, cultivate kind of a fish: the kind fish selected is put into temporary curing cement pond, and after kind fish enters pond, through 12 days slow fishes, begin to throw something and feed bait.Three, oxytocic preparation: per kilogram raun chorionic-gonadotropin hormone 1000 iu, No. 2 2ug of luteinizing hormone releasing hormone and DOM 1mg mixing match.Four, hasten parturition: the oxytocic drug that step 3 is prepared is expelled to the dorsal fin base portion muscle of parent fish.Five, artificial insemination: dry method is inseminated, and obtains germ cell.Six, hatching: obtaining the north after 7 days must loach fry.Parent fish gonadal maturation degree is basically identical.Parent fish population is concentrated and lays eggs, and improves spawning rate and rate of fertilization.Whole technical process is all to implement in controllable scope, is affected by the external environment less, convenient and reliable operation.
Description
Technical field
The invention belongs to fish culture technical field, particularly relate to the artificial fecundation method of a kind of north palpus loach.
Background technology
North palpus loach (Barbatua nuda), another name north bar loach, flower Misgurni anguillicaudati, bar loach, Dong Shi bar loach, Dong Shi palpus loach,
It is under the jurisdiction of Cypriniformes, Cobitidae, Nemacheilinae, must belong to by loach, be distributed mainly on China Yalu River, the Liaohe River, upper and middle reaches, Heilungkiang, Usu
In the water system such as river, Song Hua River, Nenjiang, Mudanjiang, the Suifenhe, TUMENJIANG RIVER, like living in cold water domain, belong to small-sized cold water fish
Class.
North palpus loach meat flavour delicacy is delicious, is deeply liked by consumer.In Losses in Eastern Mountain Area of Liao Ning, the per kilogram north must loach adult fish
Reaching 100～400 yuan, economic worth is higher, and supply falls short of demand in market.The at present market supply of this fish fishing for wild resource is still
Main.Ordered about by economic interests in recent years, place of production fisherman to this fish overfishing, the kind of wild resource in heavy damage basin
Group structure, specification and the quantity of spawning colony fall sharply, and cause its natural resources amount drastically to decline.From protection species resource and development
From the point of view of Basic-education economy, it is badly in need of artificial propagation and the oxygen-increasing reagent of this fish.But prior art does not has northern palpus
Loach Sex judging, artificial induced spawning and artificial incubation report, typically do not know about its habit, therefore cannot be carried out artificial induced spawning and incubate
Change.
Summary of the invention
It is an object of the invention to provide the artificial fecundation method of a kind of north palpus loach, will solve the existing north must loach fry
Need to fish at natural water area, have that fry quantity is few, specification is uneven, it is impossible to the problem of cultivating large area.
The technical scheme is that
The artificial fecundation method of a kind of north palpus loach, comprises the steps:
One, gather kind of a fish: fish from natural water area and select healthy wild fish, start at the beginning of 3 months to collect north palpus loach parent fish: female
Fish length 9～16cm, body weight 8～100g, more than 2 ages, abdominal part is full and transparent, can be clearly apparent mature egg grain in ovary；Male
Fish length 8～more than 15cm, body weight 5～75g, more than 1 age, hands touches with harsh feeling.With 30-40g/L saline solution dipping bath after collection
5-10 minute, female milter quantity ratio was 2～3:1.
Two, put in a suitable place to breed, cultivate kind of a fish: the kind fish selected is put into temporary curing cement pond, density be 100-150 tail/, the depth of water
40-60cm, water temperature controls at 10 DEG C-17 DEG C.After kind fish enters pond, delaying fish through 1-2 days, begin to throw something and feed bait, and bait uses granule
Feedstuff and water silk earthworm combine, and feeding volume accounts for kind of a 3-5% for fish body weight, trains improved variety fish through 3～October, the nurturing period every 3
It changes water once；
Three, oxytocic preparation: the oxytocic dose used by per kilogram raun is chorionic-gonadotropin hormone 1000 iu, rush
Ovulation No. 2 2ug and Di Ou ketone 1mg mixing match of element, milter dosage is raun 1/2；
Four, hasten parturition: the oxytocic drug that step 3 is prepared is expelled to the dorsal fin base portion muscle of parent fish, by the parent fish male and female after injection
Separately put back to storage pond, holding pond；Storage pond, holding pond water temperature is promoted to 20～25 DEG C；
Five, artificial insemination: after hastening parturition 23～28 hours, hand-dissection milter, to take out spermary, grind extrusion seminal fluid, raun is extruded
Ovum grain, essence ovum mixing, dry method is inseminated, is obtained germ cell；
Six, hatching: put in incubator after germ cell is cleaned and hatch, water temperature 18～23 DEG C, take sunshade above incubator
Net shading treatment, oxygen in water is maintained at least 5mg/L, hatches early stage, and every day rejects dead ovum and changes water once, and latter 3 days every
It changes water 2～3 times, treats that fry health is the most black, and flat trip trailing water mud sump is cultivated.Whole incubation period accumulated temperature needs 130～150 to subsist, and 7
～after 10 days, obtain north palpus loach fry.
Described temporary curing cement pond is ceramic tile shop fixtures, rectangle, area 8-10.
The present invention has the advantage that and effect:
1. parent fish is through antenatal cultivation, and gonadal maturation degree is basically identical；
2. by stimulation by running water, inject the methods such as oxytocic drug, make parent fish population concentrate and lay eggs, improve spawning rate and fertilization
Rate；
The most whole technical process is all to implement in controllable scope, is affected by the external environment less, convenient and reliable operation, just
Producing in scale of organization, preferably solving the north must a loach seed large-scale production difficult problem.By applying this technology, the north must loach
Spawning rate reaches more than 80%, and fertilization rate reached more than 60%, incubation rate reaches more than 80%, and weed survival rate reaches more than 60%.
Detailed description of the invention
Embodiment 1
The artificial fecundation method of a kind of north palpus loach, comprises the steps:
One, gather kind of a fish: fish from natural water area and select healthy wild fish, start at the beginning of 3 months to collect palpus loach parent fish from the north:
Female fish length 9～11cm, body weight 8～50g, in 3 ages, abdominal part is full and transparent, can be clearly apparent ovum grain in mature ovarian；Milter body
Long 8～more than 11cm, body weight 5～30g, in 2 ages, hands touches with harsh feeling.30g/L saline solution dipping bath is used 5 minutes, female milter after collection
Quantity is than for 2:1.
Two, put in a suitable place to breed, cultivate kind of a fish: the kind fish selected is put into temporary curing cement pond, density be 100 tails/, depth of water 40cm,
Water temperature controls at 10 DEG C.After kind fish enters pond, through 1 day slow fish, begin to throw something and feed bait, and bait uses pellet to tie mutually with water silk earthworm
Closing, feeding volume accounts for the 3% of kind of fish body weight, trains improved variety fish through March, and every 3 days of nurturing period note changes water once.
Three, oxytocic preparation: the oxytocic dose used by per kilogram raun is that chorionic-gonadotropin hormone 1000 is international single
Position, No. 2 2ug of luteinizing hormone releasing hormone and DOM 1mg mixing match, milter dosage is raun 1/2.
Four, hasten parturition: the oxytocic drug that step 3 is prepared is expelled to the dorsal fin base portion muscle of parent fish, by the parent fish after injection
Male and female separately put back to storage pond, holding pond；Storage pond, holding pond water temperature is promoted to 20 DEG C.
Five, artificial insemination: hasten parturition latter 28 hours, hand-dissection milter, to take out spermary, grind extrusion seminal fluid, raun is extruded
Ovum grain, essence ovum mixing, dry method is inseminated, is obtained germ cell.
Six, hatching: put in incubator after germ cell is cleaned and hatch, water temperature 18 DEG C, take sunshade above incubator
Net shading treatment, oxygen in water is maintained at 6mg/L, hatches early stage, rejects dead ovum every day and note changes water once, latter 3 day every day
Changing water 2 times, treat that fry health is the most black, flat trip trailing water mud sump is cultivated.Whole incubation period accumulated temperature needs 130 to subsist, and obtains after 10 days
The north must loach fry.
Described temporary curing cement pond is ceramic tile shop fixtures, rectangle, area 8.
Embodiment 2
The artificial fecundation method of a kind of north palpus loach, comprises the steps:
One, gather kind of a fish: fish from natural water area and select healthy wild fish, start at the beginning of 3 months to collect palpus loach parent fish from the north:
Female fish length 10～15cm, body weight 30～75g, in 3 ages, abdominal part is full and transparent, can be clearly apparent mature egg grain in ovary；Milter
Body length 10～more than 13cm, body weight 20～50g, in 2 ages, hands touches with harsh feeling.35g/L saline solution dipping bath is used 7 minutes after collection, female
Milter quantity is than for 2.5:1.
Two, put in a suitable place to breed, cultivate kind of a fish: the kind fish selected is put into temporary curing cement pond, density be 125 tails/, depth of water 50cm,
Water temperature controls at 15 DEG C.After kind fish enters pond, through 1 day slow fish, begin to throw something and feed bait, and bait uses pellet to tie mutually with water silk earthworm
Closing, feeding volume accounts for the 4% of kind of fish body weight, trains improved variety fish through July, and every 3 days of nurturing period changed water once.
Three, oxytocic preparation: the oxytocic dose used by per kilogram raun is that chorionic-gonadotropin hormone 1000 is international single
Position, No. 2 2ug of luteinizing hormone releasing hormone and DOM 1mg mixing match, milter dosage is raun 1/2.
Four, hasten parturition: the oxytocic drug that step 3 is prepared is expelled to the dorsal fin base portion muscle of parent fish, by the parent fish after injection
Male and female separately put back to storage pond, holding pond；Storage pond, holding pond water temperature is promoted to 23 DEG C.
Five, artificial insemination: hasten parturition latter 25 hours, hand-dissection milter, to take out spermary, grind extrusion seminal fluid, raun is extruded
Ovum grain, essence ovum mixing, dry method is inseminated, is obtained germ cell.
Six, hatching: put in incubator after germ cell is cleaned and hatch, water temperature 20 DEG C, take sunshade above incubator
Net shading treatment, oxygen in water is maintained at least 7mg/L, hatches early stage, and every day rejects dead ovum and changes water once, and latter 3 days every
It changes water 2 times, treats that fry health is the most black, and flat trip trailing water mud sump is cultivated.Whole incubation period accumulated temperature needs 140 to subsist, and obtains after 8 days
The north must loach fry.
Described temporary curing cement pond is ceramic tile shop fixtures, rectangle, area 9.
Embodiment 3
The artificial fecundation method of a kind of north palpus loach, comprises the steps:
One, gather kind of a fish: fish from natural water area and select healthy wild fish, start at the beginning of 3 months to collect north palpus loach parent fish: female
Fish length 12～16cm, body weight 75～100g, in 3 ages, abdominal part is full and transparent, can be clearly apparent mature egg grain in ovary；Milter
Body length 10～more than 15cm, body weight 50～75g, in 2 ages, hands touches with harsh feeling.40g/L saline solution dipping bath is used 10 minutes after collection,
Female milter quantity is than for 3:1.
Two, put in a suitable place to breed, cultivate kind of a fish: the kind fish selected is put into temporary curing cement pond, density be 150 tails/, depth of water 60cm,
Water temperature controls at 17 DEG C.After kind fish enters pond, through 2 days slow fishes, begin to throw something and feed bait, and bait uses pellet to tie mutually with water silk earthworm
Closing, feeding volume accounts for the 5% of kind of fish body weight, trains improved variety fish through October, and every 3 days of nurturing period changed water once.
Three, oxytocic preparation: the oxytocic dose used by per kilogram raun is that chorionic-gonadotropin hormone 1000 is international single
Position, No. 2 2ug of luteinizing hormone releasing hormone and DOM 1mg mixing match, milter dosage is raun 1/2.
Four, hasten parturition: the oxytocic drug that step 3 is prepared is expelled to the dorsal fin base portion muscle of parent fish, by the parent fish after injection
Male and female separately put back to storage pond, holding pond；Storage pond, holding pond water temperature is promoted to 25 DEG C.
Five, artificial insemination: after hastening parturition 23～28 hours, hand-dissection milter, take out spermary, grind extrusion seminal fluid, raun
Extrusion ovum grain, essence ovum mixing, dry method is inseminated, is obtained germ cell.
Six, hatching: put in incubator after germ cell is cleaned and hatch, water temperature 23 DEG C, take sunshade above incubator
Net shading treatment, oxygen in water is maintained at 7mg/L, hatches early stage, and every day rejects dead ovum and changes water once, and latter 3 day every day changed
Water 3 times, treats that fry health is the most black, and flat trip trailing water mud sump is cultivated.Whole incubation period accumulated temperature needs 135 to subsist, and obtains the north after 7 days
Must loach fry.
Described temporary curing cement pond is ceramic tile shop fixtures, rectangle, area 10.
Claims (4)
1. the artificial fecundation method of a north palpus loach, it is characterised in that comprise the steps:
One, gather kind of a fish: fish from natural water area and select healthy wild fish, start at the beginning of 3 months to collect north palpus loach parent fish: female
Fish length 9～16cm, body weight 8～100g, more than 2 ages, abdominal part is full and transparent, can be clearly apparent mature egg grain in ovary；Male
Fish length 8～more than 15cm, body weight 5～75g, more than 1 age, hands touches with harsh feeling；With 30-40g/L saline solution dipping bath after collection
5-10 minute, female milter quantity ratio was 2～3:1；
Two, put in a suitable place to breed, cultivate kind of a fish: the kind fish selected is put into temporary curing cement pond, density be 100-150 tail/, depth of water 40-
60cm, water temperature controls at 10 DEG C-17 DEG C；After kind fish enters pond, delaying fish through 1-2 days, begin to throw something and feed bait, and bait uses pellet
Combining with water silk earthworm, feeding volume accounts for kind of a 3-5% for fish body weight, trains improved variety fish through 3～October, and every 3 days of nurturing period changed
Water is once；
Three, oxytocic preparation: the oxytocic dose used by per kilogram raun is chorionic-gonadotropin hormone 1000 iu, rush
No. 2 2ug of Luteinizing hormone releasing hormone and DOM 1mg mixing match, milter dosage is raun 1/2；
Four, hasten parturition: the oxytocic drug that step 3 is prepared is expelled to the dorsal fin base portion muscle of parent fish, by the parent fish male and female after injection
Separately put back to storage pond, holding pond；Storage pond, holding pond water temperature is promoted to 20～25 DEG C；
Five, artificial insemination: after hastening parturition 23～28 hours, hand-dissection milter, to take out spermary, grind extrusion seminal fluid, raun is extruded
Ovum grain, essence ovum mixing, dry method is inseminated, is obtained germ cell；
Six, hatching: put in incubator after germ cell is cleaned and hatch, water temperature 18～23 DEG C, take sunshade above incubator
Net shading treatment, oxygen in water is maintained at least 5mg/L, hatches early stage, and every day rejects dead ovum and changes water once, and latter 3 days every
It changes water 2～3 times, treats that fry health is the most black, and flat trip trailing water mud sump is cultivated；Whole incubation period accumulated temperature needs 130～150 to subsist, and 7
～after 10 days, obtain north palpus loach fry.
The artificial fecundation method of a kind of north the most according to claim 1 palpus loach, it is characterised in that comprise the steps:
One, gather kind of a fish: fish from natural water area and select healthy wild fish, start at the beginning of 3 months to collect palpus loach parent fish from the north:
Female fish length 9～11cm, body weight 8～50g, in 3 ages, abdominal part is full and transparent, can be clearly apparent mature egg grain in ovary；Milter body
Long 8～more than 11cm, body weight 5～30g, in 2 ages, hands touches with harsh feeling；30g/L saline solution dipping bath is used 5 minutes, female milter after collection
Quantity is than for 2:1；
Two, put in a suitable place to breed, cultivate kind of a fish: the kind fish selected is put into temporary curing cement pond, density be 100 tails/, depth of water 40cm, water temperature
Control at 10 DEG C；After kind fish enters pond, through 1 day slow fish, begin to throw something and feed bait, and bait uses pellet and water silk earthworm to combine,
Feeding volume accounts for the 3% of kind of fish body weight, trains improved variety fish through March, and every 3 days of nurturing period note changes water once；
Three, oxytocic preparation: the oxytocic dose used by per kilogram raun is chorionic-gonadotropin hormone 1000 iu, rush
No. 2 2ug of Luteinizing hormone releasing hormone and DOM 1mg mixing match, milter dosage is raun 1/2；
Four, hasten parturition: the oxytocic drug that step 3 is prepared is expelled to the dorsal fin base portion muscle of parent fish, by the parent fish male and female after injection
Separately put back to storage pond, holding pond；Storage pond, holding pond water temperature is promoted to 20 DEG C；
Five, artificial insemination: hasten parturition latter 28 hours, hand-dissection milter, to take out spermary, grind extrusion seminal fluid, raun extrudes ovum grain,
Essence ovum mixing, dry method is inseminated, is obtained germ cell；
Six, hatching: put in incubator after germ cell is cleaned and hatch, water temperature 18 DEG C, take sunshade net above incubator and hide
Optical processing, oxygen in water is maintained at 6mg/L, hatches early stage, rejects dead ovum every day and note changes water once, and latter 3 day every day changed water 2
Secondary, treat that fry health is the most black, flat trip trailing water mud sump is cultivated；Whole incubation period accumulated temperature needs 130 to subsist, and obtains north palpus after 7 days
Loach fry；
Described temporary curing cement pond is ceramic tile shop fixtures, rectangle, area 8.
The artificial fecundation method of a kind of north the most according to claim 1 palpus loach, it is characterised in that comprise the steps:
One, gather kind of a fish: fish from natural water area and select healthy wild fish, start at the beginning of 3 months to collect north palpus loach parent fish: female
Fish length 10～15cm, body weight 30～75g, in 3 ages, abdominal part is full and transparent, can be clearly apparent mature egg grain in ovary；Milter body
Long 10～more than 13cm, body weight 20～50g, in 2 ages, hands touches with harsh feeling；35g/L saline solution dipping bath is used 7 minutes, male and female after collection
Fish quantity is than for 2.5:1；
Two, put in a suitable place to breed, cultivate kind of a fish: the kind fish selected is put into temporary curing cement pond, density be 125 tails/, depth of water 50cm, water temperature
Control at 15 DEG C；After kind fish enters pond, through 1 day slow fish, begin to throw something and feed bait, and bait uses pellet and water silk earthworm to combine,
Feeding volume accounts for the 4% of kind of fish body weight, trains improved variety fish through July, and every 3 days of nurturing period changed water once；
Three, oxytocic preparation: the oxytocic dose used by per kilogram raun is chorionic-gonadotropin hormone 1000 iu, rush
Luteinizing hormone releasing hormone 2ug and DOM 1mg mixing match, milter dosage is raun 1/2；
Four, hasten parturition: the oxytocic drug that step 3 is prepared is expelled to the dorsal fin base portion muscle of parent fish, by the parent fish male and female after injection
Separately put back to storage pond, holding pond；Storage pond, holding pond water temperature is promoted to 23 DEG C；
Five, artificial insemination: hasten parturition latter 25 hours, hand-dissection milter, to take out spermary, grind extrusion seminal fluid, raun extrudes ovum grain,
Essence ovum mixing, dry method is inseminated, is obtained germ cell；
Six, hatching: put in incubator after germ cell is cleaned and hatch, water temperature 20 DEG C, take sunshade net above incubator and hide
Optical processing, oxygen in water is maintained at 7mg/L, hatches early stage, and every day rejects dead ovum and changes water once, and latter 3 day every day changed water 2
Secondary, treat that fry health is the most black, flat trip trailing water mud sump is cultivated；Whole incubation period accumulated temperature needs 140 to subsist, and obtains north palpus after 8 days
Loach fry；
Described temporary curing cement pond is ceramic tile shop fixtures, rectangle, area 9.
The artificial fecundation method of a kind of north the most according to claim 1 palpus loach, it is characterised in that comprise the steps:
One, gather kind of a fish: fish from natural water area and select healthy wild fish, start at the beginning of 3 months to collect north palpus loach parent fish: female
Fish length 12～16cm, body weight 75～100g, in 3 ages, abdominal part is full and transparent, can be clearly apparent mature egg grain；Male fish length 10
～more than 15cm, body weight 50～75g, in 2 ages, hands touches with harsh feeling；40g/L saline solution dipping bath is used 10 minutes, female milter after collection
Quantity is than for 3:1；
Two, put in a suitable place to breed, cultivate kind of a fish: the kind fish selected is put into temporary curing cement pond, density be 150 tails/, depth of water 60cm, water temperature
Control at 17 DEG C；After kind fish enters pond, through 2 days slow fishes, begin to throw something and feed bait, and bait uses pellet and water silk earthworm to combine,
Feeding volume accounts for the 5% of kind of fish body weight, trains improved variety fish through October, and every 3 days of nurturing period changed water once；
Three, oxytocic preparation: the oxytocic dose used by per kilogram raun is chorionic-gonadotropin hormone 1000 iu, rush
No. 2 2ug of Luteinizing hormone releasing hormone and DOM 1mg mixing match, milter dosage is raun 1/2；
Four, hasten parturition: the oxytocic drug that step 3 is prepared is expelled to the dorsal fin base portion muscle of parent fish, by the parent fish male and female after injection
Separately put back to storage pond, holding pond；Storage pond, holding pond water temperature is promoted to 25 DEG C；
Five, artificial insemination: hasten parturition latter 23 hours, hand-dissection milter, to take out spermary, grind extrusion seminal fluid, raun extrudes ovum grain,
Essence ovum mixing, dry method is inseminated, is obtained germ cell；
Six, hatching: put in incubator after germ cell is cleaned and hatch, water temperature 23 DEG C, take sunshade net above incubator and hide
Optical processing, oxygen in water is maintained at 7mg/L, hatches early stage, and every day rejects dead ovum and changes water once, and latter 3 day every day changed water 3
Secondary, treat that fry health is the most black, flat trip trailing water mud sump is cultivated；Whole incubation period accumulated temperature needs 150 to subsist, and obtains north palpus after 10 days
Loach fry；
Described temporary curing cement pond is ceramic tile shop fixtures, rectangle, area 10.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107047401A (en) * | 2017-04-28 | 2017-08-18 | 贵州省水产研究所 | A kind of mountain area loach parent culture and induced spawning method and hatching system |
| CN107296177A (en) * | 2017-08-01 | 2017-10-27 | 辽宁省淡水水产科学研究院 | A kind of north must loach carp compound feed |
| CN107711611A (en) * | 2017-10-17 | 2018-02-23 | 铜仁职业技术学院 | A kind of low damage of Chinese snake head, high-efficiency artificial propagation method |
| CN108739541A (en) * | 2018-06-14 | 2018-11-06 | 吴林 | A kind of Misgurnus auguillicaudatus autumn and winter propagation method |
| CN110583538A (en) * | 2019-09-19 | 2019-12-20 | 云南省渔业科学研究院 | Artificial propagation method of Misgurni multiflori |
| CN114982678A (en) * | 2022-07-21 | 2022-09-02 | 新疆额河生态养殖科技有限公司 | Artificial breeding operating system for fennel fish |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047401A (en) * | 2017-04-28 | 2017-08-18 | 贵州省水产研究所 | A kind of mountain area loach parent culture and induced spawning method and hatching system |
| CN107296177A (en) * | 2017-08-01 | 2017-10-27 | 辽宁省淡水水产科学研究院 | A kind of north must loach carp compound feed |
| CN107711611A (en) * | 2017-10-17 | 2018-02-23 | 铜仁职业技术学院 | A kind of low damage of Chinese snake head, high-efficiency artificial propagation method |
| CN108739541A (en) * | 2018-06-14 | 2018-11-06 | 吴林 | A kind of Misgurnus auguillicaudatus autumn and winter propagation method |
| CN110583538A (en) * | 2019-09-19 | 2019-12-20 | 云南省渔业科学研究院 | Artificial propagation method of Misgurni multiflori |
| CN114982678A (en) * | 2022-07-21 | 2022-09-02 | 新疆额河生态养殖科技有限公司 | Artificial breeding operating system for fennel fish |
| CN114982678B (en) * | 2022-07-21 | 2024-02-23 | 新疆额河生态养殖科技有限公司 | Artificial breeding operation method for arctic fennel |
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