CN105684962B - One kind sticks up mouth Culter and triangular bream hybrid preparation method - Google Patents
One kind sticks up mouth Culter and triangular bream hybrid preparation method Download PDFInfo
- Publication number
- CN105684962B CN105684962B CN201510787390.6A CN201510787390A CN105684962B CN 105684962 B CN105684962 B CN 105684962B CN 201510787390 A CN201510787390 A CN 201510787390A CN 105684962 B CN105684962 B CN 105684962B
- Authority
- CN
- China
- Prior art keywords
- water
- parent
- triangular bream
- mouth culter
- sticks
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The present invention provides one kind to stick up mouth Culter and triangular bream hybrid preparation method, this method includes the sport technique segments such as Juvenile stage, reinforced cultivating, artificial induced spawning, artificial insemination, hatching and cultivation, the present invention has stronger operability, the cenospecies clean-cut and growth vigor obtained is obvious, and have a sweet temper, jump is not liked, and resistance is strong, it is easy to catch, fingerling and adult fish long-distance transport survival rate are high；Pierce few compared with mouth Culter is stuck up between flesh, and muscle delicacy degree is better than triangular bream, and Fresh ear field content is higher；With more extensive eating habit, more excellent lower oxygen concentration resistance and anti-stress ability；Cenospecies both sexes are fertile, and possibility is provided further to excavate the cultivation potentiality of hybrid filial generation, are expected to cultivate the cultivation new varieties that character is excellent and stabilization is hereditary.
Description
Technical field
The invention belongs to fish hybridization breeding technical field, is specifically that one kind sticks up mouth Culter and triangular bream hybrid preparation method.
Background technology
Crossbreeding is a kind of effective ways of quick improvement culture trait.According to statistics, between existing at least 1,000 kinds of fish
Hybrid experiment has been carried out, but the hybridization first filial generation only between a few species shows obvious heterosis, hybrid vigor, and in actual production
In be widely applied（Such as bolti × Sarotherodon sp, Ban Channa × Wu Channa）.Consider chromosome number, caryoplasm parent
And the problems such as property, having can be hybridized between the most of species of scholarly forecast Culter subfamilies two-by-two.Stick up mouth Culter, triangular bream is to be subordinate to respectively
Belong to the Important Economic fish that Culter subfamilies Culter belongs to and triangular bream belongs to, the former is pelagic fishes, and build is elongated, carnivorous, it is naturally a
Body is big, fine and tender taste, economic value are high, but thorn is more between flesh, it is of high cost and edgy to propagate artificially, is not easy fishing and live fish
Transport；And the latter is middle lower floor fish, build is generous, it also has not only compared with megalobrama amblycephala fine and tender taste, nutritive value height etc.
The features such as feeding habits of megalobrama amblycephala are miscellaneous, aquaculture cost is low, disposition is docile, easy fishing and live fish list, but to cultivation water requirement compared with
Height, its management level are more demanding.It is mutual in chromosome number, reproduction, shape, feeding habits and resistance etc. to stick up mouth Culter and triangular bream
Benefit property is strong, its cenospecies has obvious super close heterosis, hybrid vigor, and market prospects are good.At present, CN101720698B discloses one kind
Megalobrama amblycephala and stick up the method for mouth Culter distant hybridizations, 102893938 A of CN disclose a kind of Xenocypris davidi and it is remote between mouth Culter subfamilies to stick up
A kind of method that the method for edge hybridization, CN101884314B disclose grass carp and megalobrame amblycephala subfamily distant hydridization, but it is above-mentioned
Hybridizing method is to sticking up mouth Culter and triangular bream intermolecular hybrid and not applying to.
The content of the invention
Present invention aims at establish the breeding method that sticks up hybridizing method and hybrid seedlings between mouth Culter and triangular bream.For
Reach above-mentioned technical purpose, present invention employs following technical scheme：
One kind sticks up mouth Culter and triangular bream hybrid preparation method, and this method includes following sport technique segment：
（1）Juvenile stage：Select the defect individual through continuous multi-generation selection and breeding or excellent induced through continuous gynogenesis
Body is as parent.
（2）Reinforced cultivating：The reinforced cultivating includes parent and sticks up the reinforced cultivating of mouth Culter and the reinforcing training of parent's triangular bream
Educate：
(I) mouth Culter parent's reinforced cultivatings are stuck up：It is selected to stick up mouth Culter parents and put female-male proportion in a suitable place to breed as 1:1.5-2, stocking rate
For 200-300kg/667m2；Daily ration of feeding is the 2%~5% of fish body weight；Mid-April, the new water of note is rushed weekly 1-2 times, 5
The last ten-days period moon stop note bath.
(II) triangular bream parent reinforced cultivating：It is selected to stick up mouth Culter parents and put female-male proportion in a suitable place to breed as 1：1.2-1.5, puts in a suitable place to breed
Measure as 100-150kg/667m2.Daily ration of feeding is the 3%~7% of fish body weight；Early April starts to fill weekly new water 1 time, often
Secondary bath 2-3h；Stop bath when close to the bread worm, and male and female parent population point pond is cultivated.
（3）Artificial induced spawning：Selected from reinforced cultivating pond and complete the parent population of reinforced cultivating and inject oxytocic drug, every kilogram sticks up
Mouth Culter raun HCG injection 1000-2000 IU and LRH-A25-10 μ g intermixtures, milter dosage halve；Every kilogram of triangular bream is female
Fish injection DOM 3-5mg and LRH-A210-20 μ g intermixtures, milter dosage halve；Parent population is put by sex ration 3: 1-3 after injection
Enter different egg pond to hasten parturition.
（4）Artificial insemination：Using raun a kind of in two kinds of parent fishes be maternal and another milter as male parent, light pressure is female
This parent population belly is squeezed into fish-egg in the china bowl dried, while male parent parent population sperm is squeezed into the china bowl for filling fish-egg, with dry
Feather is gently mixed 1~2 minute, finally pours into uniformly mixed smart ovum and unsticking is carried out in ready slime water, clear water is washed
After net, it is put into hatching cylinder or circular hatching channal.
（5）Hatching：It is 30~50 × 10 to hatch seed density in cylinder4Tail/m3, seed density is in circular hatching channal
50~80 × 104Tail/m3, hatching current are not deposited with fish-egg to be advisable, after fry can put down trip in water, you can and band water is emerged, into
Summer has set in flower seedling kind breeding phase.
（6）Seed rearing：
(I) pond pre-treatment：10~15d before fry pond-inputting, the dry pool is sterilized；2~3d after disinfection, new water is filtered in injection to be made
The depth of water is 40~50cm, per 667m2Apply 100~200kg of fermentation organic fertilizer；
(II) fry rearing：After seed pond-inputting, soya bean of uniformly splashing daily starches 1.5~2kg/10, ten thousand tails, and cultivation density is
10~200,000 tails/667m2；Fry is grown to 2cm, and increase feeds mash feed, daily 2~3kg/667m2；Pond-inputting after a week, often
3d~5d filling filters new water once, and each depth of water increases by 10~15cm, until fry is grown to more than 3.0cm.
Parent population quality is the key of artificial hybridization, in order to ensure hybrid generation uniformity of type and can more show to hybridize excellent
Gesture, the present invention are typically chosen the defect individual through continuous multi-generation selection and breeding, or select the defect individual induced through continuous gynogenesis.
The quality of parent's reinforced cultivating is directly related to ovum quality and seed vigor, and core link requirement feeds parent population and match somebody with somebody except enough
Close outside feed, should also periodically feed a certain amount of fresh food and bath stimulates, to promote parent's gonad development and maturation.Adopt
With oxytocic hormone to complete reinforced cultivating stick up mouth Culter and triangular bream parent hastens parturition, in view of sticking up mouth Culter and triangular bream to hastening parturition
The sensitiveness of hormone is different, and also there is notable difference in the effector phase under the conditions of the oxytocic drug combination, dosage and the certain water temperature that use,
Dose of medicament and time of hastening parturition are particularly significant；In addition, triangular bream and mouth Culter is stuck up there are reproduction isolation, in natural environmental condition
Lower both are difficult to direct natural mating, it is necessary to use artificial insemination, and stick up that mouth Culter is edgy, irritability is big, if undue artificial
Interference, it is difficult to easily cause female parent population breeding, it is therefore necessary to accurately hold triangular bream and stick up the estrus time of mouth Culter, in time collection
Smart ovum carries out artificial insemination.
Preferably, step（1）The parent is age 3-4 full age, more than weight 1.0kg and physical strong, disease-free nothing
Wound sticks up mouth Culter and triangular bream.
Preferably, step（2）In feed stick up mouth Culter parents feed bait with disease-free small dimension fresh food fish
Based on, and it is aided with high-quality expanded pellet feed；The bait for feeding triangular bream parent is mainly feed, spiral shell a species of small clam living in fresh water of auxiliary addition grating
Deng fresh bait.
Preferably, step（3）The female male parent that middle screening is used to hasten parturition is specially to select to have completed reinforced cultivating
Parent population, raun requirement head and belly are smooth, belly expands softness, both sides ovary profile is obvious and gonopore is micro- red；Milter will
Head and body surface hand is asked to touch with harsh feeling, belly does not expand, light to press belly to have the outflow of milky sperm.
Preferably, step（3）In time of specifically hastening parturition be late May to mid-June, water temperature is not less than 23 DEG C.Triangle
The repoductive time of triangular bream is early and middle ten days in May in In Middle And Lower Reaches of Changjiang River, and more than 20 DEG C of water temperature, most suitable water temperature of hastening parturition is 24 DEG C~28
℃.Stick up the repoductive time of mouth Culter is advisable with early June to mid-July, more than 23 DEG C of water temperature, and suitable water temperature is 25-28 DEG C.With reference to
Produce reality, the present invention recommend both the Best Times of hybridization should select in late May to mid-June, more than 23 DEG C of water temperature.
Table 1 sticks up mouth Culter and triangular bream injection oxytocic drug dosage and Effect time at different temperatures compares
Preferably, step（3）In stick up mouth Culter prior to triangular bream 1-2h inject oxytocic drug.Effect time is direct with water temperature
Correlation, sticking up the ecbolic effect time of mouth Culter and triangular bream at different temperatures is shown in Table 1.As seen from the table, during 24-25 DEG C of water temperature,
It is 9-10h, triangular bream 8-9h to stick up mouth Culter Effect times；During 26-28 DEG C of water temperature, it is 7-8.5h to stick up mouth Culter Effect times, triangle
Triangular bream is 6-7h.Therefore, both, which hybridize, generally requires to stick up mouth Culter prior to injecting oxytocic drug after triangular bream 1-2h.
Preferably, step（4）When parent population chases in spawning pond in " helical form ", appropriate ovum is speckled with water outlet mesh sheet
During grain, there is the raun that ovum is smoothly flowed out from cloacal aperture to carry out artificial insemination when choosing light pressure fish belly portion both sides.Due to triangular bream
With stick up mouth Culter reproduction isolate, under natural environmental condition both be difficult to direct natural mating, stick up in addition mouth Culter it is edgy, should
It is big to swash property, if undue human interference, it is difficult to easily cause female parent population breeding（Mature egg can not be extruded）.Therefore, it should accurately handle
Hold triangular bream and stick up the estrus time of mouth Culter, the smart ovum of collection in time carries out artificial insemination.It is general to require when parent population is in spawning pond
After being chased in " helical form ", appropriate ovum grain being speckled with water outlet mesh sheet, seine detection is carried out, during such as light pressure raun belly both sides,
Ovum is smoothly flowed out from cloacal aperture, you can carries out artificial insemination.
Preferably, step（6）The hatching water is to be more than or equal to through the filtering of 100~120 mesh Double-layer screens and dissolved oxygen
6mg/L's crosses drainage.
Preferably, step（6）The disinfection is specially with 75~150kg/667m2Quick lime do the pool disinfection；It is described
It is the water through 60~80 mesh silk cover filterings to filter new water.After a week, per 3d~5d, filling filters new water once, every time for fry pond-inputting
The depth of water increases by 10~15cm.After the depth of water reaches 80~100cm, using water transfer mode, transparency is set to be maintained at 20~30cm.
In conclusion the invention has the advantages that：
1. the invention has stronger operability.Due to sticking up the gamete of mouth Culter and triangular bream（Sperm and ovum）Between fit
Good with property, the fertility rate and hatchability that both hybridize is higher（More than 80%）, such as normal bisexual hybridition, there is mass supply
The hereditary basis of hybrid seedlings.On the other hand, the hybridizing method which establishes, it can be ensured that stick up mouth Culter and triangular bream parent
Synchronous ripe, synchronization of estrus, can obtain substantial amounts of ovum and sperm the same period and be hybridized, and be applicable to the big rule of hybrid seedlings
Mould produces.
2. the cenospecies clean-cut that the invention obtains.Cenospecies build is in long shuttle-type, and body colour is similar to female parent and sticks up mouth
Culter, small, body is thick, scale is thin, the accreditation in readily available market.Table 2-3 is the 2 age Culter triangular bream F randomly selected1And its Parent
Denumerable, metric characters data, display hybrid majority character is between Parent.
Table 2：Stick up mouth Culter（♀）× triangular bream（♂）F1And its Parent metric characters compare Data-Statistics（Unit：cm）
Table 3：Stick up mouth Culter（♀）× triangular bream（♂）F1And its denumerable character statistics of Parent（Unit：Piece, bar or a）
3. the cenospecies growth vigor that the invention obtains is obvious.Relative to its parent, the schistostoma of cenospecies, the gill, pharynx tooth,
The features such as enteron aisle, scale show that it has more extensive eating habit, lower oxygen concentration resistance and resisting stress energy between Parent
Power.Hybrid F1Neat specification is homogeneous, and average growth rate improves 73.6% compared with mouth Culter fast 32.5%, cultured output is stuck up；Hybrid F1Both may be used
The higher puffing material of forage protein of ingesting, can also ingest the relatively low particulate material of the forage proteins such as carp, crucian, breeding feed coefficient
Between 1-1.25；Have a sweet temper, do not like jump, resistance is strong, is easy to catch, and fingerling and adult fish long-distance transport survival rate are high；
Pierce few compared with mouth Culter is stuck up between flesh, and muscle delicacy degree is better than triangular bream, and Fresh ear field content is higher.
4. the cenospecies both sexes that the invention obtains are fertile.It is different from most distant hybridization filial generation infertility etc., hybrid F1Property
Gland can reach maturity and can natural propagation, self propagated filial performance goes out obvious trait segregation.On the other hand, hybrid is fertile
Property also for further excavate hybrid filial generation cultivation potentiality provide possibility, by the lasting selection and breeding be selfed, be returned, be expected to cultivate
Go out the cultivation new varieties that character is excellent and stabilization is hereditary.
Figure of description
Fig. 1 sticks up mouth Culter for hybrid strain.
Fig. 2 is hybrid strain triangular bream.
Fig. 3 is hybridization F1For Culter triangular breams.
Embodiment
The realization, functional characteristics and beneficial effect of the object of the invention are further illustrated below in conjunction with specific embodiment and attached drawing
Fruit.This specific embodiment is only explanation of the invention, it is not limitation of the present invention, and those skilled in the art are readding
Running through as needed can make the present embodiment the modification of no creative contribution after this specification, but as long as the present invention's
All protected in right be subject to Patent Law.
Embodiment 1
First, crossing phase
1. Juvenile stage：The defect individual through continuous multi-generation selection and breeding is selected to stick up mouth Culter and triangular bream year as hybrid strain
Age reaches 3 full ages, more than weight 1.0kg, and physical strong, disease-free and injury-free.
2. reinforced cultivating：
（1）Stick up the reinforced cultivating of mouth Culter parents：Female-male proportion is put in a suitable place to breed as 1:1.5, stocking rate 200kg/667m2.Feed
Bait is aided with high-quality expanded pellet feed based on disease-free small dimension fresh food fish, and daily ration of feeding is total weight
2%.Mid-April, the new water of punching note 1 time, late May stopping note being washed by water weekly.
（2）The reinforced cultivating of triangular bream parent：Female-male proportion is put in a suitable place to breed as 1：1.2, stocking rate 100kg/667m2.Except throwing
Feeding material is outer, also adds spiral shell a species of small clam living in fresh water of grating, and daily ration of feeding is the 3% of total weight.New water is filled since the early April weekly 1 time,
Bath 2h every time.During close to its bread worm, stop bath, and male and female parent population point pond is cultivated.
3. artificial induced spawning：
（1）Female male parent differentiates：Parent population directly is captured from reinforced cultivating pond, head is selected and belly is smooth, belly expands
Softness, both sides ovary profile is obvious, gonopore it is micro- it is red stick up mouth Culter rauns and head and body surface hand touches with harsh feeling, belly is not swollen
Greatly, gently pressure belly has the triangular bream milter that milky sperm flows out.
（2）Oxytocic drug is injected：When late May, more than 23 DEG C of water temperature, oxytocic drug is injected to hybrid strain, sticks up mouth
Culter injects oxytocic drug prior to triangular bream 1h.It is per 1000 IU and LRH-A of kg body weight HCG injection to stick up mouth Culter rauns dosage2
5 μ g intermixtures；Triangular bream milter dosage is that DOM 1.5mg and LRH-A are injected per kg body weight25 μ g intermixtures.Using abdominal cavity one
Secondary property injection, parent population presses sex ration 3 after injection：1, which is put into different egg pond, hastens parturition.Keep bath and the certain water of spawning pond
Stream, until parent population high-volume heat.
4. artificial insemination：When parent population chases in spawning pond in " helical form ", appropriate ovum grain is speckled with water outlet mesh sheet
Afterwards, seine detection is carried out, choosing light pressure fish belly both sides has ovum to stick up mouth Culter rauns from what cloacal aperture smoothly flowed out, is blocked with thumb
Its cloacal aperture, then dried its belly and cloacal aperture surrounding with dry towel, it is light to press belly to squeeze into fish-egg in the china bowl dried, together
When take out triangular bream milter and squeeze into sperm in the china bowl for filling fish-egg, be gently mixed 1 minute with dry feather, finally will be mixed
The uniform smart ovum of conjunction, which pours into, carries out unsticking in ready slime water, after clear water is cleaned, be put into Lotic hatching in hatching cylinder.
2nd, hatching and breeding phase
5. hatching.Hatching water is filtered through 100 mesh Double-layer screens, dissolved oxygen 6mg/L, is hatched seed density in cylinder and is
30~50 × 104Tail/m3, hatching current are not deposited with fish-egg to be advisable, after fry can put down trip in water, you can and band water is emerged, into
Summer has set in flower seedling kind breeding phase.
6. seed rearing：10d before fry pond-inputting, uses 75kg/667m2Quick lime do the pool disinfection；2d after disinfection, injection
New water 40cm through 60 mesh silk cover filterings, per 667m2Apply fermented organic fertilizer 100kg；After seed pond-inputting, uniformly splash daily
Soya bean is starched, and ten thousand tails of 1.5kg/10, cultivation density is 100,000 tails/667m2, fry grown to 2cm, and increase feeds fish meal, daily
2kg/667m2, divide the morning, afternoon to feed for 2 times, pond-inputting after a week, per 3d filling once, each 10cm.After depth of water 80cm, use
Water transfer mode, makes transparency be maintained at 20cm.
When fry is grown to more than 3.0cm, Culter triangular breams F1Seed is bred as.
Embodiment 2
First, crossing phase
1. Juvenile stage：The defect individual through continuous multi-generation selection and breeding is selected to stick up mouth Culter and triangular bream year as hybrid strain
Age reaches 4 full ages, more than weight 1.0kg, and physical strong, disease-free and injury-free.
2. reinforced cultivating：
（1）Stick up the reinforced cultivating of mouth Culter parents：Female-male proportion is put in a suitable place to breed as 1:1.8, stocking rate 250kg/667m2.Feed
Bait is aided with high-quality expanded pellet feed based on disease-free small dimension fresh food fish, and daily ration of feeding is total weight
3%.Mid-April, the new water of punching note 2 times, late May stopping note being washed by water weekly.
（2）The reinforced cultivating of triangular bream parent：Female-male proportion is put in a suitable place to breed as 1：1.3, stocking rate 130kg/667m2.Except throwing
Feeding material is outer, also adds spiral shell a species of small clam living in fresh water of grating, and daily ration of feeding is the 5% of total weight.New water is filled since the early April weekly 1 time,
Bath 2.5h every time.During close to its bread worm, stop bath, and male and female parent population point pond is cultivated.
3. artificial induced spawning：
（1）Female male parent differentiates：Parent population directly is captured from reinforced cultivating pond, head is selected and belly is smooth, belly expands
Softness, both sides ovary profile is obvious, and the micro- red triangular bream raun of gonopore and head and body surface hand touch with harsh feeling, and belly is not swollen
Greatly, gently pressure belly have milky sperm flow out stick up mouth Culter milters.
（2）Oxytocic drug is injected：Early June injects oxytocic drug when more than 25 DEG C of water temperature, to hybrid strain, sticks up mouth
Culter injects oxytocic drug prior to triangular bream 1.5h.It is per 750 IU and LRH-A of kg body weight HCG injection to stick up mouth Culter milters dosage2
4 μ g intermixtures；Triangular bream raun dosage is that DOM 4mg and LRH-A are injected per kg body weight215 μ g intermixtures.Using abdominal cavity one
Secondary property injection, parent population presses sex ration 3 after injection：2, which are put into different egg pond, hastens parturition.Keep bath and the certain water of spawning pond
Stream, until parent population high-volume heat.
4. artificial insemination：When parent population chases in spawning pond in " helical form ", appropriate ovum grain is speckled with water outlet mesh sheet
Afterwards, seine detection is carried out, choosing light pressure fish belly both sides has the triangular bream raun that ovum is smoothly flowed out from cloacal aperture, is blocked with thumb
Cloacal aperture, then dried its belly and cloacal aperture surrounding with dry towel, it is light to press belly to squeeze into fish-egg in the china bowl dried, at the same time
Taking-up sticks up mouth Culter milters and squeezes into sperm in the china bowl for filling fish-egg, and 1.5 minutes are gently mixed with dry feather, finally will mixing it is equal
Even smart ovum, which pours into, carries out unsticking in ready slime water, after clear water is cleaned, be put into Lotic hatching in hatching cylinder.
2nd, hatching and breeding phase
5. hatching.Hatching water is filtered through 110 mesh Double-layer screens, dissolved oxygen 6mg/L, is hatched seed density in cylinder and is
50×104Tail/m3, hatching current are not deposited with fish-egg to be advisable, after fry can put down trip in water, you can band water is emerged, into summer has set in
Flower seedling kind breeding phase.
6th, seed rearing：12d before fry pond-inputting, uses 100kg/667m2Quick lime do the pool disinfection；3d after disinfection, injection
New water 45cm through 70 mesh silk cover filterings, per 667m2Apply fermented organic fertilizer 150kg；After seed pond-inputting, uniformly splash daily
Soya bean is starched, and ten thousand tails of 1.8kg/10, cultivation density is 150,000 tails/667m2, fry grown to 2cm, and increase feeds bean cake powder, daily
2.5kg/667m2, divide the morning, afternoon to feed for 2 times, pond-inputting after a week, per 4d filling once, each 12cm.After depth of water 90cm, adopt
With water transfer mode, transparency is set to be maintained at 25cm.
When fry is grown to more than 3.0cm, Culter triangular breams F1Seed is bred as.
Embodiment 3
First, crossing phase
1. Juvenile stage：Select the excellent triangular bream through continuous multi-generation selection and breeding individual and through the induction of continuous gynogenesis
The excellent mouth Culter individuals that stick up are used as hybrid strain, stick up mouth Culter and the triangular bream age reaches 4 full ages, more than weight 1.0kg, and constitution is good for
It is strong, disease-free and injury-free.
2. reinforced cultivating：
（1）Stick up the reinforced cultivating of mouth Culter parents：Female-male proportion is put in a suitable place to breed as 1:1.8, stocking rate 250kg/667m2.Feed
Bait is aided with high-quality expanded pellet feed based on disease-free small dimension fresh food fish, and daily ration of feeding is total weight
3%.Mid-April, the new water of punching note 2 times, late May stopping note being washed by water weekly.
（2）The reinforced cultivating of triangular bream parent：Female-male proportion is put in a suitable place to breed as 1：1.3, stocking rate 130kg/667m2.Except throwing
Feeding material is outer, also adds spiral shell a species of small clam living in fresh water of grating, and daily ration of feeding is the 5% of total weight.New water is filled since the early April weekly 1 time,
Bath 2.5h every time.During close to its bread worm, stop bath, and male and female parent population point pond is cultivated.
3. artificial induced spawning：
（1）Female male parent differentiates：Parent population directly is captured from reinforced cultivating pond, head is selected and belly is smooth, belly expands
Softness, both sides ovary profile is obvious, gonopore it is micro- it is red stick up mouth Culter rauns and head and body surface hand touches with harsh feeling, belly is not swollen
Greatly, gently pressure belly has the triangular bream milter that milky sperm flows out.
（2）Oxytocic drug is injected：Early June injects oxytocic drug when more than 25 DEG C of water temperature, to hybrid strain, sticks up mouth
Culter injects oxytocic drug prior to triangular bream 1.5h.It is per 1500 IU and LRH- of kg body weight HCG injection to stick up mouth Culter rauns dosage
A28 μ g intermixtures；Triangular bream milter dosage is that DOM 2mg and LRH-A are injected per kg body weight27 μ g intermixtures.Using abdominal cavity
Disposable injection, parent population presses sex ration 3 after injection：3, which are put into different egg pond, hastens parturition.Keep bath and the certain water of spawning pond
Stream, until parent population high-volume heat.
4. artificial insemination：When parent population chases in spawning pond in " helical form ", appropriate ovum grain is speckled with water outlet mesh sheet
Afterwards, seine detection is carried out, choosing light pressure fish belly both sides has ovum to stick up mouth Culter rauns from what cloacal aperture smoothly flowed out, is blocked with thumb
Cloacal aperture, then dried its belly and cloacal aperture surrounding with dry towel, it is light to press belly to squeeze into fish-egg in the china bowl dried, at the same time
Take out triangular bream milter to squeeze into sperm in the china bowl for filling fish-egg, be gently mixed 1.5 minutes, will finally mixed equal with dry feather
Even smart ovum, which pours into, carries out unsticking in ready slime water, after clear water is cleaned, be put into Lotic hatching in circular hatching channal.
2nd, hatching and breeding phase
5. hatching.Hatching water is filtered through 110 mesh Double-layer screens, dissolved oxygen 6mg/L, seed density in circular hatching channal
For 50 × 104Tail/m3, hatching current are not deposited with fish-egg to be advisable, after fry can put down trip in water, you can and band water is emerged, into
Summer has set in flower seedling kind breeding phase.
6th, seed rearing：12d before fry pond-inputting, uses 100kg/667m2Quick lime do the pool disinfection；3d after disinfection, injection
New water 45cm through 70 mesh silk cover filterings, per 667m2Apply fermented organic fertilizer 150kg；After seed pond-inputting, uniformly splash daily
Soya bean is starched, and ten thousand tails of 1.8kg/10, cultivation density is 150,000 tails/667m2, fry grown to 2cm, and increase feeds bean cake powder, daily
2.5kg/667m2, divide the morning, afternoon to feed for 2 times, pond-inputting after a week, per 4d filling once, each 12cm.After depth of water 90cm, adopt
With water transfer mode, transparency is set to be maintained at 25cm.
When fry is grown to more than 3.0cm, Culter triangular breams F1Seed is bred as.
Embodiment 4
First, crossing phase
1. Juvenile stage：Select the excellent triangular bream through continuous multi-generation selection and breeding individual and through the induction of continuous gynogenesis
The excellent mouth Culter individuals that stick up are used as hybrid strain, stick up mouth Culter and the triangular bream age reaches 4 full ages, more than weight 1.0kg, and constitution is good for
It is strong, disease-free and injury-free.
2. reinforced cultivating：
（1）Stick up the reinforced cultivating of mouth Culter parents：Female-male proportion is put in a suitable place to breed as 1:2, stocking rate 300kg/667m2.Feed bait
Material is aided with high-quality expanded pellet feed based on disease-free small dimension fresh food fish, and daily ration of feeding is the 5% of total weight.
Mid-April, the new water of punching note 2 times, late May stopping note being washed by water weekly.
（2）The reinforced cultivating of triangular bream parent：Female-male proportion is put in a suitable place to breed as 2：3, stocking rate 150kg/667m2.Except feeding
Outside feed, spiral shell a species of small clam living in fresh water of grating is also added, daily ration of feeding is the 7% of total weight.New water is filled since the early April weekly 1 time, often
Secondary bath 3h.During close to its bread worm, stop bath, and male and female parent population point pond is cultivated.
3. artificial induced spawning：
（1）Female male parent differentiates：Parent population directly is captured from reinforced cultivating pond, head is selected and belly is smooth, belly expands
Softness, both sides ovary profile is obvious, gonopore it is micro- it is red stick up mouth Culter rauns and head and body surface hand touches with harsh feeling, belly is not swollen
Greatly, gently pressure belly has the triangular bream milter that milky sperm flows out.
（2）Oxytocic drug is injected：Mid-June, during more than 28 DEG C of water temperature, injects oxytocic drug to hybrid strain, sticks up mouth Culter
Oxytocic drug is injected prior to triangular bream 2h.It is per 2000 IU and LRH-A of kg body weight HCG injection to stick up mouth Culter rauns dosage2 10
μ g intermixtures；Triangular bream milter dosage is that DOM 2.5mg and LRH-A are injected per kg body weight210 μ g intermixtures.Using abdominal cavity
Disposable injection, parent population presses sex ration 3 after injection：2, which are put into different egg pond, hastens parturition.Keep bath and the certain water of spawning pond
Stream, until parent population high-volume heat.
4. artificial insemination：When parent population chases in spawning pond in " helical form ", appropriate ovum grain is speckled with water outlet mesh sheet
Afterwards, seine detection is carried out, choosing light pressure fish belly both sides has ovum to stick up mouth Culter rauns from what cloacal aperture smoothly flowed out, is blocked with thumb
Cloacal aperture, then dried its belly and cloacal aperture surrounding with dry towel, it is light to press belly to squeeze into fish-egg in the china bowl dried, at the same time
Take out triangular bream milter to squeeze into sperm in the china bowl for filling fish-egg, be gently mixed 2 minutes with dry feather, will be finally uniformly mixed
Smart ovum pour into unsticking carried out in ready slime water, after clear water is cleaned, be put into Lotic hatching in circular hatching channal.
2nd, hatching and breeding phase
5. hatching.Hatching water is filtered through 120 mesh Double-layer screens, more than dissolved oxygen 6mg/L, and seed is close in circular hatching channal
Spend for 80 × 104Tail/m3, hatching current are not deposited with fish-egg to be advisable, after fry can put down trip in water, you can and band water is emerged,
Into the summer flower seed rearing stage.
6th, seed rearing：15d before fry pond-inputting, uses 150kg/667m2Quick lime do the pool disinfection；3d after disinfection, injection
New water 50cm through 80 mesh silk cover filterings, per 667m2Apply fermented organic fertilizer 200kg；After seed pond-inputting, uniformly splash daily
Soya bean is starched, and ten thousand tails of 2kg/10, cultivation density is 200,000 tails/667m2, fry grown to 2cm, and increase feeds fish meal, daily 3kg/
667m2, divide the morning, afternoon to feed for 2 times, pond-inputting after a week, per 5d filling once, each 15cm.After depth of water 100cm, using tune
Water mode, makes transparency be maintained at 30cm.
When fry is grown to more than 3.0cm, Culter triangular breams F1Seed is bred as.
Claims (10)
1. one kind sticks up mouth Culter and triangular bream hybrid preparation method, it is characterised in that this method includes following sport technique segment：
（1）Juvenile stage：The defect individual through continuous multi-generation selection and breeding or the defect individual through the induction of continuous gynogenesis is selected to make
For parent；
（2）Reinforced cultivating：The reinforced cultivating sticks up the reinforced cultivating of mouth Culter and the reinforced cultivating of parent's triangular bream including parent：
(I) mouth Culter parent's reinforced cultivatings are stuck up：It is selected to stick up mouth Culter parents and put female-male proportion in a suitable place to breed as 1:1.5-2 stocking rate is
200-300kg /667m2；Daily ration of feeding is the 2%~5% of fish body weight；Mid-April, the new water of note is rushed weekly 1-2 times, May
The last ten-days period stop note bath；
(II) triangular bream parent reinforced cultivating：Selected triangular bream parent puts female-male proportion in a suitable place to breed as 1：1.2-1.5 stocking rate is
100-150kg /667m2,
Daily ration of feeding is the 3%~7% of fish body weight；Early April starts to fill weekly new water 1 time, and wash by water 2-3h every time；Close to numerous
Stop bath when growing temperature, and male and female parent population point pond is cultivated；
（3）Artificial induced spawning：Selected from reinforced cultivating pond and complete the parent population of reinforced cultivating and inject oxytocic drug, every kilogram sticks up mouth Culter
Raun HCG injection 1000-2000 IU and LRH-A25-10 μ g intermixtures, milter dosage halve；Every kilogram of triangular bream raun note
Penetrate DOM 3-5mg and LRH-A210-20 μ g intermixtures, milter dosage halve；Parent population is put into not by sex ration 3: 1-3 after injection
Hasten parturition with spawning pond；
（4）Artificial insemination：Using raun a kind of in two kinds of parent fishes be maternal and another milter as male parent, the light maternal parent of pressure
Fish belly portion squeezes into fish-egg in the china bowl dried, while male parent parent population sperm is squeezed into the china bowl for filling fish-egg, with dry feather
It is gently mixed 1~2 minute, finally uniformly mixed smart ovum is poured into unsticking is carried out in ready slime water, after clear water is cleaned,
It is put into hatching cylinder or circular hatching channal；
（5）Hatching：It is 30~50 × 10 to hatch seed density in cylinder4Tail/m3, in circular hatching channal seed density for 50~
80×104Tail/m3, hatching current are not deposited with embryonated egg to be advisable；After fry can put down trip in water, you can band water is emerged, and is entered
The summer flower seed rearing stage；
（6）Seed rearing：
(I) pond pre-treatment：10~15d before fry pond-inputting, the dry pool is sterilized；2~3d after disinfection, new water is filtered in injection makes the depth of water
For 40~50cm, every 667m2Apply 100~200kg of fermentation organic fertilizer；
(II) fry rearing：After seed pond-inputting, soya bean of uniformly splashing daily slurry 1.5~2kg/10, ten thousand tails, cultivation density for 10~
200000 tails/667m2；Fry is grown to 2cm, and increase feeds mash feed, daily 2~3kg/667m2；Pond-inputting after a week, per 3d~
5d filling filters new water once, and each depth of water increases by 10~15cm, until fry is grown to more than 3.0cm.
2. one kind as claimed in claim 1 sticks up mouth Culter and triangular bream hybrid preparation method, step（1）The parent is age 3-4
Full age, more than weight 1.0kg and physical strong, disease-free and injury-free stick up mouth Culter and triangular bream.
3. one kind as claimed in claim 1 sticks up mouth Culter and triangular bream hybrid preparation method, step（2）Feed the bait for sticking up mouth Culter parents
Material includes disease-free fresh food fish and expanded pellet feed；Feeding the bait of triangular bream parent includes feed and fresh bait.
4. one kind as claimed in claim 1 sticks up mouth Culter and triangular bream hybrid preparation method, step（3）In female male parent screening tool
Body is to capture parent population from reinforced cultivating pond, selects head and belly is smooth, belly expands that soft, both sides ovary profile is obvious and raw
Grow the raun of Kong Weihong；Select head and body surface hand touches with harsh feeling, belly does not expand, light to press belly to have the outflow of milky sperm
Milter.
5. one kind as claimed in claim 1 sticks up mouth Culter and triangular bream hybrid preparation method, step（3）The specific time of hastening parturition is May
The last ten-days period to mid-June, water temperature are not less than 23 DEG C.
6. one kind as claimed in claim 1 sticks up mouth Culter and triangular bream hybrid preparation method, step（3）The mouth Culter that sticks up is prior to triangle
Triangular bream 1-2h injects oxytocic drug.
7. one kind as claimed in claim 1 sticks up mouth Culter and triangular bream hybrid preparation method, step（4）When parent population is in spawning pond
" helical form " is chased, when appropriate ovum grain is speckled with water outlet mesh sheet, has the ovum suitable from cloacal aperture when choosing light pressure fish belly portion both sides
The raun of profit outflow carries out artificial insemination.
8. one kind as claimed in claim 1 sticks up mouth Culter and triangular bream hybrid preparation method, step（5）The hatching water is through 100
~120 mesh Double-layer screens filter and dissolved oxygen crosses drainage more than or equal to 6mg/L.
9. one kind as claimed in claim 1 sticks up mouth Culter and triangular bream hybrid preparation method, step（6）The disinfection is specially with 75
~150kg/667m2Quick lime do the pool disinfection；The new water of filtering is the water through 60~80 mesh silk cover filterings.
10. one kind as claimed in claim 1 sticks up mouth Culter and triangular bream hybrid preparation method, step（6）After the middle depth of water reaches 80cm,
Using water transfer mode, transparency is set to be maintained at 20~30cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510787390.6A CN105684962B (en) | 2015-11-17 | 2015-11-17 | One kind sticks up mouth Culter and triangular bream hybrid preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510787390.6A CN105684962B (en) | 2015-11-17 | 2015-11-17 | One kind sticks up mouth Culter and triangular bream hybrid preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105684962A CN105684962A (en) | 2016-06-22 |
| CN105684962B true CN105684962B (en) | 2018-05-11 |
Family
ID=56228127
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510787390.6A Active CN105684962B (en) | 2015-11-17 | 2015-11-17 | One kind sticks up mouth Culter and triangular bream hybrid preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105684962B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548715A (en) * | 2018-12-05 | 2019-04-02 | 中国海洋大学 | A kind of northern area flower perch whole artificial propagation technology |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577388B (en) * | 2016-12-14 | 2019-08-06 | 华中农业大学 | A kind of cross breeding method of excellent No. 1 Pelteobagrus fulvidraco of Huang of improvement growth traits |
| CN106868112B (en) * | 2017-01-20 | 2019-12-06 | 浙江省淡水水产研究所 | molecular identification method for culter alburnus, triangular bream and filial generation thereof |
| CN107347747A (en) * | 2017-09-05 | 2017-11-17 | 上海海洋大学 | A kind of construction method for growing the hybridization triangular bream Culter pierced between fast and few flesh |
| CN108925472B (en) * | 2018-07-02 | 2020-11-13 | 华中农业大学 | Cross breeding method for elopichthys bambusa and megalobrama amblycephala |
| CN109220901A (en) * | 2018-09-20 | 2019-01-18 | 杭州市农业科学研究院 | A kind of triangular bream cultivation water ecological control method |
| CN111480600A (en) * | 2019-01-29 | 2020-08-04 | 射阳县周正海家庭农场 | Reproduction method for bream and culter hybrid amphiphilic gene recombination |
| CN111657186B (en) * | 2020-05-28 | 2022-02-18 | 湖南师范大学 | Method for cultivating natural gynogenesis megalobrama amblycephala |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1518864A (en) * | 2003-09-02 | 2004-08-11 | 天津市换新水产良种场 | Method of distant hybridization between subfamilies of cyprinid fish |
| CN101720698A (en) * | 2009-12-09 | 2010-06-09 | 湖南师范大学 | Method for distant hybridization of megalobrama amblycephala and erythroculter ilishaeformis |
| CN101884314A (en) * | 2010-07-26 | 2010-11-17 | 湖南师范大学 | Method for inter-subfamily distant hybridization of grass carp and megalobrama amblycephala |
| CN102893938A (en) * | 2012-11-12 | 2013-01-30 | 湖南师范大学 | Subfamily distant hybridization method for Xenocypris davidi Bleeker and Erythroculter ilishaeformis Bleeker |
| CN103190368A (en) * | 2013-04-09 | 2013-07-10 | 武汉先锋水产科技有限公司 | Breeding method for distant hybridization between erythroculter ilishaeformis and ancherythropterus nigrocauda |
-
2015
- 2015-11-17 CN CN201510787390.6A patent/CN105684962B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1518864A (en) * | 2003-09-02 | 2004-08-11 | 天津市换新水产良种场 | Method of distant hybridization between subfamilies of cyprinid fish |
| CN101720698A (en) * | 2009-12-09 | 2010-06-09 | 湖南师范大学 | Method for distant hybridization of megalobrama amblycephala and erythroculter ilishaeformis |
| CN101884314A (en) * | 2010-07-26 | 2010-11-17 | 湖南师范大学 | Method for inter-subfamily distant hybridization of grass carp and megalobrama amblycephala |
| CN102893938A (en) * | 2012-11-12 | 2013-01-30 | 湖南师范大学 | Subfamily distant hybridization method for Xenocypris davidi Bleeker and Erythroculter ilishaeformis Bleeker |
| CN103190368A (en) * | 2013-04-09 | 2013-07-10 | 武汉先锋水产科技有限公司 | Breeding method for distant hybridization between erythroculter ilishaeformis and ancherythropterus nigrocauda |
Non-Patent Citations (3)
| Title |
|---|
| The fertility of the hybrid lineage derived from female Megalobrama amblycephala × male Culter alburnus;Jun Xiao,etc.;《Animal Reproduction Science》;20141230;第61-70页 * |
| 团头鲂(♀)×翘嘴鲌(♂)杂交后代的遗传特征及生长差异;郑国栋等;《中国水产科学》;20150515;第22卷(第3期);第402-409页 * |
| 鲂属4种鱼类种间杂交的初步研究;张大龙等;《大连海洋大学学报》;20140415;第29卷(第2期);第121-125页 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548715A (en) * | 2018-12-05 | 2019-04-02 | 中国海洋大学 | A kind of northern area flower perch whole artificial propagation technology |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105684962A (en) | 2016-06-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105684962B (en) | One kind sticks up mouth Culter and triangular bream hybrid preparation method | |
| CN100464633C (en) | Erythtoculer ilishaefornis and megajobrama amblycephala crossbreeding method | |
| CN102217560A (en) | Loach fry artificial breeding method | |
| CN103828750B (en) | The breeding method of loach prelarva | |
| CN105660465A (en) | Artificial young lobster breeding method | |
| CN106212333A (en) | A kind of artificial fecundation method of north palpus loach | |
| CN106577393A (en) | Artificial propagation method for breeding acrossocheilus fasciatus | |
| CN104719199A (en) | Cross breeding method for pelteobagrus fulvidraco and pseudobagrus vachelli | |
| CN101960996A (en) | Artificial breeding method of Scaphesthes macrolepis fry | |
| CN104642212A (en) | Artificial breeding method for onychostoma simus | |
| CN103461254A (en) | Scale artificial propagation method for procypris rabaudi | |
| CN101707986B (en) | Pelteobagrus vachelli and pseudobagrus ussuriensis cross breeding method | |
| CN101223865B (en) | Seed production and culture method for cyprinoid with short growth circle | |
| CN102524128B (en) | Artificial reproduction method for Pseudobagras ussuriensis | |
| CN103493767B (en) | Odontobulis mpotamophila and Odontobutis yaluensis cross breeding method | |
| CN101161064A (en) | A cultivation method of oolong crucian and ink dragon carp | |
| CN103843710A (en) | Breeding and culture method of erythroculter ilishaeformis | |
| CN103222436A (en) | Artificial propagation method of esox reichertii | |
| CN104160992A (en) | Ecological breeding method for grass carps | |
| CN106804482A (en) | A kind of artificial fecundation method of autumn Pelteobagrus fulvidraco seedling | |
| CN102599095A (en) | Estrus and spawning induction method for increasing rate of artificial insemination of Schizothorax davidi (Sauvage) | |
| CN107439424A (en) | A kind of production of hybrid seeds of Jin Xinwu crucian carps and cultural method | |
| CN106614120A (en) | Pseudobagrus fulvidraco fry culture method | |
| CN108834957A (en) | A kind of method of bolti and Mandarin fish gynogenesis | |
| CN104381168A (en) | Rapid propagation method for mud fish |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |