CN102599095A

CN102599095A - Estrus and spawning induction method for increasing rate of artificial insemination of Schizothorax davidi (Sauvage)

Info

Publication number: CN102599095A
Application number: CN2012101056931A
Authority: CN
Inventors: 杨勇; 杨永志
Original assignee: YULONG LONGQUAN AQUACULTURE TECHNOLOGY Co Ltd
Current assignee: YULONG LONGQUAN AQUACULTURE TECHNOLOGY Co Ltd
Priority date: 2012-04-12
Filing date: 2012-04-12
Publication date: 2012-07-25
Anticipated expiration: 2032-04-12
Also published as: CN102599095B

Abstract

The invention provides an estrus and spawning induction method for increasing the rate of artificial insemination of Schizothorax davidi (Sauvage), which includes estrus induction of parent fishes, spawning induction and insemination. The method is characterized by including: firstly, well mixing pituitary excretion of mature fishes of Schizothorax davidi (Sauvage) and distilled water according to a mass ratio of 1:50-70 before parent fishes of Schizothorax davidi (Sauvage) lay eggs, and injecting the male and female parent fishes with the mixture to induce estrus; secondly, injecting spawning liquid to the root of a pectoral fin of each parent fish to induce spawning when genital orifices of the female parent fishes flush slightly or tail fins of the female parent fishes flush under observation; and thirdly, collecting sperms and the eggs for insemination, mixing the sperms and the eggs according to a ratio of 1.5-2:1, and placing the mixture into fresh water for cleaning.

Description

A kind of method of hastening parturition of promoting the sexual maturity that improves heavy mouth schizothoracin artificial breed rate

Technical field

The invention belongs to a kind of artificial incubation method of heavy mouth schizothoracin, be specifically related to improve the method for hastening parturition of promoting the sexual maturity of heavy mouth schizothoracin artificial breed rate.

Background technology

Heavy mouth schizothoracin (Schizothorax (Racoma) davidi) belongs to Cypriniformes, Cyprinidae, Schizothoracinae, Schizothorax; Split buttocks fish subgenus, popular name: Chong Kou, heavy lip schizothoracin are the rare indigenous cold water fishes of China; Because of its fine and tender taste, meat flavour is delicious, favored by consumers in general.Wild heavy mouth schizothoracin milter sexual maturity during three ages, raun sexual maturity during four ages, August from laying eggs during adverse current to distributary in each great river; Lean on the extruding of water temperature and milter to stimulate and lay eggs,, particularly be subject to the influence of surrounding environment so egg-laying time is not concentrated; Seed distribution randomness is strong, and the fertilization rate of ovum is not high, the influence of factor such as changed by natural environment, human transition is fished for; Heavy mouth schizothoracin nature population reduces in a large number, and is endangered.。

People begin to propagate artificially the artificial incubation method that weighs the mouth schizothoracin and begin one's study heavy rictus abdomen fish parent fish in recent years; The reproduction rate of heavy rictus abdomen fish parent fish improves; But adopt under the spontaneous estrus situation, artificial exerting pressure gather fish-egg and milt submethod; Cause seminal fluid and maturation of ovum rate not high like this, thereby make the insemination rate of fish-egg not high.

Summary of the invention

The objective of the invention is to the present not high problem of heavy mouth schizothoracin fertilization rate, a kind of artificial incubation that improves the heavy mouth schizothoracin of insemination rate method of hastening parturition of promoting the sexual maturity is provided.

The present invention is achieved in that and comprises and promote the sexual maturity, hasten parturition and inseminate, it is characterized in that:

1, promote the sexual maturity: the sexually matured heavy mouth schizothoracin that will choose in 7～August is put into parent fish pond flowing water and is put in a suitable place to breed, high 180～200 ㎝ in parent fish pond pond, and the depth of water 60～80 ㎝, the water temperature of flowing water is that 9～15 ℃, dissolved oxygen amount are that 6～10 ㎎/L, pH value are 6.5～7.5; Laid eggs preceding 40～50 hours in; After ripe carp brain hypophysis and distilled water mix by mass ratio 1:50～70; Be expelled to the pectoral fin root of male and female parent population; Carp brain hypophysis and distilled water mixed liquor injection volume are pressed 2～3% of body weight for the dam fish, and male parent population is by body weight 0.8～1.2%, and injection back male and female parent population is separately put in a suitable place to breed.

2, hasten parturition: the gonopore of observing heavy rictus abdomen dam fish rubescent or male parent population mouth slightly has corn to occur and tail fin when reddening, the injection liquid of hastening parturition at parent population pectoral fin root place, and male parent population injection 1 time, dam fish injection twice, the time interval is 10～16 hours; The liquid of hastening parturition is the preparation according to the ratio of volume ratio 1:0.8～1.2 of human chorionic gonadtropin (HCG) and maleic acid DOM (DOM), the injection volume of the liquid of hastening parturition be male parent population by body weight 0.8～1.2%, the dam fish is by body weight 1.8～2.4%; The male and female parent population is separately put in a suitable place to breed in the injection back.

3, insemination: gather fish-egg and milt when parent population begins to lay eggs immediately, milt and fish-egg are put into the clear water rinsing by in basin, stirring with feather after the mixed of 1.5～2:1 after half a minute, each and every one absorb water full until fish-egg and epidermis seminal fluid mucus completely without.

Use heavy mouth schizothoracin of the present invention to promote the sexual maturity and hasten parturition method; Promoted the gonad development of parent population, improved the maturing rate of interior ovum of parent population body or sperm, the spawning rate of milter reaches 92%; The spawning rate of raun reaches 60%, has improved the synchronism of male parent population row essence and female parent population ovulation period simultaneously.Use the present invention, improved the quality and quantity of interior sperm of parent population body and ovum effectively, promoted the raising of insemination rate, the unit cost of production that effectively reduces.More concentrated because of egg-laying time, can realize heavy mouth schizothoracin artificial incubation industrial production requirement.

Embodiment:

1, promotes the sexual maturity: will select individual sexually matured heavy mouth schizothoracin even, that build is graceful, vigor is stronger to put into parent fish pond flowing water in 7～August and put in a suitable place to breed; High 180～200 ㎝ in parent fish pond pond; The depth of water 60～80 ㎝, the water temperature of flowing water is that 9～15 ℃, dissolved oxygen amount are that 6～10 ㎎/L, pH value are 6.5～7.5; Laid eggs preceding 40～50 hours in; After ripe carp brain hypophysis and distilled water mix by mass ratio 1:50～70; Be expelled to the pectoral fin root of male and female parent population; Carp brain hypophysis and distilled water mixed liquor injection volume are pressed 2～4% of body weight for the dam fish, and male parent population is by body weight 1～2%, and injection back male and female parent population is separately put in a suitable place to breed.

2, hasten parturition: slight when rubescent at the gonopore of observing raun, the injection ocyodinic guarantees that gather the unified time of fish-egg, when the milter tail fin reddens, also injects ocyodinic, promotes the generation of seminal fluid; Inject the liquid of hastening parturition at parent population pectoral fin root place, male parent population injection gets final product for 1 time, dam fish injection twice, and the time interval is 10～16 hours; The liquid of hastening parturition is the preparation according to the ratio of volume ratio 1:0.8～1.2 of human chorionic gonadtropin (HCG) and maleic acid DOM (DOM), the injection volume of the liquid of hastening parturition be male parent population by body weight 0.8～1.2%, the dam fish is by body weight 1.8～2.4%; The male and female parent population is separately put in a suitable place to breed in the injection back.

3, insemination: the parent population of injecting ocyodinic generally began to lay eggs about 24 hours; Apparatus such as the feather that is ready to sterilize, half-dried towel, basin; Find that parent population has that the unusual and tail fin of a mood is complete can adopt the ovum semen collection when rubescent; The half-dried towel of usefulness under the tail on the male and female fish head snarled mention, and dab the belly of fish along the fish body, gather fish-egg and milt with hand; Milt and fish-egg are put into the clear water rinsing by in basin, stirring with feather after the mixed of 1.5～2:1 after half a minute, each and every one absorb water full until fish-egg and epidermis seminal fluid mucus completely without.

Claims

1. method of hastening parturition of promoting the sexual maturity that improves heavy mouth schizothoracin artificial breed rate comprises the parent population step of promoting the sexual maturity, hasten parturition, inseminate, and it is characterized in that:

(1) promote the sexual maturity: the sexually matured heavy mouth schizothoracin that will choose in 7～August is put into parent fish pond flowing water and is put in a suitable place to breed, high 180～200 ㎝ in parent fish pond pond, and the depth of water 60～80 ㎝, the water temperature of flowing water is that 9～15 ℃, dissolved oxygen amount are that 6～10 ㎎/L, pH value are 6.5～7.5; Laid eggs preceding 40～50 hours in; After ripe carp brain hypophysis and distilled water mix by mass ratio 1:50～70; Be expelled to the pectoral fin root of male and female parent population; Carp brain hypophysis and distilled water mixed liquor injection volume are pressed 2～3% of body weight for the dam fish, and male parent population is by body weight 0.8～1.2%, and injection back male and female parent population is separately put in a suitable place to breed;

(2) hasten parturition: observe when heavily slight rubescent the or male parent population mouth of gonopore of rictus abdomen dam fish has corn appearance and tail fin to redden; Inject the liquid of hastening parturition at parent population pectoral fin root place; Male parent population injection 1 time, dam fish injection twice, the time interval is 10～16 hours; The liquid of hastening parturition is the preparation according to the ratio of volume ratio 1:0.8～1.2 of human chorionic gonadtropin (HCG) and maleic acid DOM (DOM), the injection volume of the liquid of hastening parturition be male parent population by body weight 0.8～1.2%, the dam fish is by body weight 1.8～2.4%; The male and female parent population is separately put in a suitable place to breed in the injection back;

(3) insemination: gather fish-egg and milt when parent population begins to lay eggs immediately; In basin, stir after milt and the mixed of fish-egg with feather by 1.5～2:1; Put into the clear water rinsing after half a minute, full and the epidermis seminal fluid mucus of each and every one absorbing water until fish-egg completely without.