CN103444611B - Artificial propagation method of Gymnodiptychus pachycheilus - Google Patents
Artificial propagation method of Gymnodiptychus pachycheilus Download PDFInfo
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- CN103444611B CN103444611B CN201310449874.0A CN201310449874A CN103444611B CN 103444611 B CN103444611 B CN 103444611B CN 201310449874 A CN201310449874 A CN 201310449874A CN 103444611 B CN103444611 B CN 103444611B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses an artificial propagation method of Gymnodiptychus pachycheilus. The method includes the steps of firstly, parent fish artificial raising and cultivation; secondly, mature parent fish selection and induced spawning, and semi-dry artificial insemination; thirdly, cage incubation or still water incubation. By the method, high fertility rate, incubation rate and survival rate are achieved.
Description
technical field:
The present invention is relevant with the naked skin-carp meat artificial fecundation method of thick lip.
background technology:
The naked skin-carp meat of thick lip (
gymnodiptychus pachycheilus) belong to Cypriniformes, Cyprinidae, Schizothoracinae, naked skin-carp meat belongs to.Be commonly called as: heavy lip flower fish, numb fish, parmelia saxatilis fish.Inhabit in the broad valley river, plateau of the province the Changjiang river such as Qinghai, Gansu, Sichuan and each water system of the upper reaches of the Yellow River, in river bend, Hui Shuichu is more common.Because living environment worsens and people be overfishing, add its a kind of group of mean people, distributed areas are narrow, and its growth rate is slow, age at sexual maturity is slow, the kind that genus severe cold areas is lived.The naked skin-carp meat resource of thick lip sharply declines in recent years, has been put into < < China species Red List > >, is fish in imminent danger.In order to save this resource, be not exterminated, taking artificial propagation is a kind of effective way, but up to now still without any the message to the naked skin-carp meat successful reproduction of thick lip, also without any document, shows to have people once to carry out similarly work.
summary of the invention:
The object of the invention is in order to provide the thick lip that a kind of fertilization rate, incubation rate and survival rate are high naked skin-carp meat artificial fecundation method.
The object of the present invention is achieved like this:
The naked skin-carp meat artificial fecundation method of the thick lip of the present invention, the method adopts following steps:
(1) parent population artificial domestication and cultivation;
A. the artificial domestication of parent population;
1. water source: after the clear pond sterilization of pond, groundwater abstraction is injected into 1.2-1.5 meters of depth of waters;
2. tame and docile food: the water earthworm of throwing something and feeding in advance, then throw something and feed and rub water earthworm and mash feed mixture, then the protein wt percentage composition of throwing something and feeding is 40%～42% tame and docile food artifical compound feed;
3. the method for throwing something and feeding: first adopt full pond to spill and throw something and feed, after the former carp of rock ingests in groups, progressively dwindle again the scope of spilling, finally the bait table arranging and near throw something and feed;
4. raise together with: the parent population that field is collected is raised together with quantity and reaches 1:2 together with raising together with the fish after raising and train;
B. the cultivation of parent population:
1. the naked skin-carp meat of the thick lip of antenatal reinforced cultivating is liked flowing water environment, in every day in 2～March, inject new water and reach 1/3～1/2 of pond volume, from the last ten-days period in the last ten-days period in March to May, strengthening gradually current stimulates, every Tianchong water filling 1 time, each 2～3 hours, the later stage made pond flowing water in 0.05～0.1m/s according to actual conditions
3state, meanwhile, throws something and feeds feed of animal origin as main for parent population sexual gland being reached maturity in good ecotope and trophic level, should take, and is aided with artifical compound feed, and day throwing amount of raising is 5%～10% of parent population weight, throws something and feeds every day 1～2 time;
2. postpartum breeding phase due to mate and oviposit behavior, physical consumption is larger, add the operations such as antenatal repeating fished for, carries, screened, injection and also can cause in various degree injured of parent population, in order to reduce the lethality of parent population in postpartum, parent population was all placed in the pond that water quality is pure and fresh and raised 15～20 days postpartum, enter Chi Qianying and carry out strict sterilization, to injured, compared with severe one, take intramuscular injection penicillin and with erythromycin, smear injury and processed, and regularly to pond waters sterilization, intramuscular injection penicillin dosage 10,000 units/kilogram 1～2 milliliter;
(2) selection of mature brood fish with hasten parturition:
A. the selection of mature brood fish:
Selection physique is healthy and strong, anosis without wound, take 6～8 age sexually matured parent population as main; The belly of raun expands softness, and ovary profile is obvious, and gonopore swelling is slightly micro-red; Milter has star-pursuing in blocks at rhynchodaenm front end, and fingerprint has coarse sensation, with have gentle hands, presses belly to have seminal fluid to flow out, and after water breakthrough, scatters immediately, is creamy white;
B. the selection of oxytocic drug and preparation:
Ocyodinic is selected human chorionic gonadtropin (HCG), short Luteinizing hormone releasing hormone analog (LRH-A
2) and DOM (DOM) mix to use: the raun dosage of hastening parturition is controlled at LRH-A
2: 8 μ g/ ㎏～15 μ g/ ㎏, HCG:800 IU/ ㎏～1200IU/ ㎏, DOM:3 mg/ ㎏～5mg/ ㎏ mixes use, double injection spacing 10h～12h, the milter injection that reduces by half;
C. adopt semi-dry process artificial insemination: obvious when occurring that milter chases raun, and start to answer back to its belly while oestrusing sign, catch out parent population, after raun body surface water is dried, in basin, hold at least dilution of 100ml, then squeeze ovum engagement, clamp-on seminal fluid simultaneously, stir 2～3 min, sperm is fully contacted with ovum, add again at least 500mL of the clear water identical with Spawning water, activate sperm and ovum, continue to stir after at least 1 min, add the clean unnecessary seminal fluid of 500mL clear water rinsing and foreign material, pour incubator box hatching into, dilution formula and preparation method: NaCl:85～95g, KCl:2.4～2.8g, CaCl
2: 3.0～4.0g, incorporates in 10L distilled water,
(3) hatching: adopt net cage hatching or Lentic hatching:
A. adopt net cage hatching, the depth of water that net cage is placed is at least 5cm, and Spawning water is well water, dissolved oxygen >=5mg/L, and 9-16 ℃ of hatching water temperatures, pH value is 7.2-7.8, places 1 layer of ovum in case;
B. adopt Lentic hatching, in hatchery, will add oxygen replenishing equipment, want duty to change water in hatching process, change water water temperature and water quality and oxygen content at every turn and be equal to Spawning water, in hatching, should select in time dead ovum, incubator box will be avoided illumination and vibrations;
C. maintain aforementioned hatching process until prelarva yolk sac absorbs completely, yolk sac should be thrown something and fed yolk water after filtering until flat trip after disappearing, and hatching process finishes.
While carrying out artificial insemination in said method, should note avoiding sunlight direct irradiation sperm and ovum.
The artificial propagation method fertilization rate of the present invention, incubation rate and survival rate are high, and through the test more than 3 years, the artificial propagation method fertilization rate of the present invention can reach more than 85%, and incubation rate and survival rate can reach more than 70%, can have the naked skin-carp meat resource of the thick lip in efficient recovery the upper reaches of the Yellow River.
embodiment:
The naked skin-carp meat artificial fecundation method of the thick lip of the present embodiment, the method adopts following steps:
(1) parent population artificial domestication and cultivation;
A. the artificial domestication of parent population;
1. after the clear pond sterilization of pond, water source, groundwater abstraction is injected into 1.2-1.5 meters of depth of waters;
2. tame and docile the food water earthworm of throwing something and feeding in advance, then throw something and feed and rub water earthworm and mash feed mixture, then throw something and feed and tame and docile food artifical compound feed (protein content 40%～42%);
3. the mode of throwing something and feeding first adopts full pond to spill and throws something and feeds, and progressively dwindles the scope of spilling after the former carp of rock ingests in groups again, finally the bait table arranging and near throw something and feed;
4. raise together with together with the parent population that field is collected raises together with the fish after raising and train, raise together with quantity and reach 1:2;
B. the cultivation of parent population:
1. antenatal reinforced cultivating: the naked skin-carp meat of thick lip is liked flowing water environment,, in every day in 2～March, inject new water and reach 1/3～1/2 of pond volume, from the last ten-days period in the last ten-days period in March to May, strengthening gradually current stimulates, every Tianchong water filling 1 time.Each 2.5 hours.Later stage makes pond flowing water in 0.05～0.1m/s according to actual conditions
3state, meanwhile, throws something and feeds feed of animal origin as main for parent population sexual gland being reached maturity in good ecotope and trophic level, should take, and is aided with artifical compound feed, and day throwing amount of raising is 8% of parent population weight, throws something and feeds every day 2 times, and feed is all placed in bait table;
2. postpartum breeding phase: parent population was all placed in the pond that water quality is pure and fresh and raised 15～20 days postpartum, enter Chi Qianying and carry out strict sterilization, to injured, compared with severe one, take intramuscular injection penicillin, dosage 10,000 units/kilogram 1～2 milliliter and smear injury with erythromycin and processed, and regularly pond waters is sterilized;
(2) selection of mature brood fish with hasten parturition:
A. the selection of mature brood fish:
Select the healthy and strong of physique anosis without Shang take 6～8 age sexually matured parent population as main.The belly of raun expands softness, and ovary profile is obvious, and gonopore swelling is slightly micro-red; Milter has star-pursuing in blocks at rhynchodaenm front end, and fingerprint has coarse sensation, with have gentle hands, presses belly to have seminal fluid to flow out, and after water breakthrough, scatters immediately, is creamy white;
B. the selection of oxytocic drug and preparation:
Ocyodinic is selected human chorionic gonadtropin (HCG), short Luteinizing hormone releasing hormone analog (LRH-A2) and DOM (DOM) to mix and is used.The raun dosage of hastening parturition is controlled at LRH-A2:10g/ ㎏, HCG:900 IU/ ㎏, and DOM:4mg/ ㎏ mixes use, double injection spacing 11h, the milter injection that reduces by half;
C. adopt semi-dry process artificial insemination: obvious when occurring that milter chases raun, and start to answer back to its belly while oestrusing sign, catch out parent population, after raun body surface water is dried, in basin, hold the dilution of 100mL, then squeeze ovum engagement, clamp-on seminal fluid simultaneously, with feather, stir 2.5min, sperm is fully contacted with ovum, add again the clear water 500mL identical with Spawning water, activate sperm and ovum, continue to stir after 1min, add the clean unnecessary seminal fluid of 500mL clear water rinsing and foreign material, pour incubator box hatching into, dilution formula and preparation method: Nacl:90g; Kcl:2.6g; Cacl
2: 3.5g, incorporates in 10L distilled water;
(3) hatching: adopt net cage hatching or Lentic hatching:
A. adopt net cage hatching, the depth of water that net cage is placed is 5cm, and Spawning water is well water, dissolved oxygen >=5mg/L, and 12 ℃ of hatching water temperatures, left and right pH value is 7.5, places 1 layer of ovum in case;
B. adopt Lentic hatching, in hatchery, will add oxygen replenishing equipment, want duty to change water in hatching process, change water water temperature and water quality and oxygen content at every turn and be equal to Spawning water, in hatching, should select in time dead ovum, incubator box will be avoided illumination and vibrations;
C. maintain aforementioned hatching process until prelarva yolk sac absorbs completely, yolk sac should be thrown something and fed yolk water after filtering until flat trip after disappearing, and hatching process finishes;
When carrying out artificial insemination, should avoid sunlight direct irradiation sperm and ovum.
Above-described embodiment is that foregoing of the present invention is further described, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to above-described embodiment.All technology realizing based on foregoing all belong to scope of the present invention.
Claims (2)
1. the naked skin-carp meat artificial fecundation method of thick lip, the method adopts following steps:
(1) parent population artificial domestication and cultivation:
The artificial domestication of parent population:
1. water source: after the clear pond sterilization of pond, groundwater abstraction is injected into 1.2～1.5m depth of water;
2. tame and docile food: the water earthworm of throwing something and feeding in advance, then throw something and feed and rub water earthworm and mash feed mixture, then the protein wt percentage composition of throwing something and feeding is 40%～42% tame and docile food artifical compound feed;
3. feeding method: first adopt full pond to spill and throw something and feed, after the naked skin-carp meat of thick lip is ingested in groups, progressively dwindle again the scope of spilling, finally the bait table arranging and near throw something and feed;
4. raise together with: the parent population that field is collected is raised together with quantity and reaches 1: 2 together with raising together with the fish after raising and train;
The cultivation of parent population:
1. antenatal reinforced cultivating: the naked skin-carp meat of thick lip is liked flowing water environment, in every day in 2～March, inject new water and reach 1/3～1/2 of pond volume, from the last ten-days period in the last ten-days period in March to May, strengthening gradually current stimulates, every Tianchong water filling 1 time, each 2～3 hours, make pond flowing water in 0.05～0.1m/s
3state, meanwhile, take and throw something and feed feed of animal origin as main, is aided with artifical compound feed, and day throwing amount of raising is 5%～10% of parent population weight, throws something and feeds every day 1～2 time;
2. postpartum breeding phase: parent population was all placed in the pond that water quality is pure and fresh and raised 15～20 days postpartum, enter Chi Qianying and carry out strict sterilization, to injured, compared with severe one, take intramuscular injection penicillin and with erythromycin, smear injury and processed, and regularly to pond waters sterilization, intramuscular injection penicillin dosage 10,000 units/kilogram 1～2 milliliter;
(2) selection of mature brood fish with hasten parturition:
A. the selection of mature brood fish:
Selection physique is healthy and strong, anosis without wound, take 6～8 age sexually matured parent population as main;
B. the selection of oxytocic drug and preparation:
Ocyodinic is selected human chorionic gonadtropin, short Luteinizing hormone releasing hormone analog and DOM to mix and is used, the raun dosage of hastening parturition is controlled at short Luteinizing hormone releasing hormone analog: 8 μ g/ ㎏～15 μ g/ ㎏, human chorionic gonadtropin: 800 IU/ ㎏～1200IU/ ㎏, DOM: 3 mg/ ㎏～5mg/ ㎏ mix use, double injection spacing 10h～12h, the milter injection that reduces by half;
C. adopt semi-dry process artificial insemination: obvious when occurring that milter chases raun, and start to answer back to its belly while oestrusing sign, catch out parent population, after raun body surface water is dried, in basin, hold at least dilution of 100mL, then squeeze ovum engagement, clamp-on seminal fluid simultaneously, stir 2～3 min, sperm is fully contacted with ovum, add again at least 500mL of the clear water identical with Spawning water, activate sperm and ovum, continue to stir at least after 1min, add the clean unnecessary seminal fluid of 500ml clear water rinsing and foreign material, pour incubator box hatching into, dilution formula and preparation method: NaCl:85～95g, KCl:2.4～2.8g, CaCl
2: 3.0～4.0g, incorporates in 10L distilled water,
(3) hatching: adopt net cage hatching or Lentic hatching:
A. adopt net cage hatching: the depth of water that net cage is placed is at least 5cm, and Spawning water is well water, dissolved oxygen >=5mg/L, 9-16 ℃ of hatching water temperatures, pH value is 7.2-7.8, places 1 layer of ovum in case;
B. adopt Lentic hatching: in hatchery, will add oxygen replenishing equipment, want duty to change water in hatching process, change water water temperature and water quality and oxygen content at every turn and be equal to Spawning water, in hatching, should select in time dead ovum, incubator box will be avoided illumination and vibrations;
C. maintain aforementioned hatching process until prelarva yolk sac absorbs completely, yolk sac should be thrown something and fed yolk water after filtering until flat trip after disappearing, and hatching process finishes.
2. the naked skin-carp meat artificial fecundation method of thick lip as claimed in claim 1, while it is characterized in that carrying out artificial insemination, should note avoiding sunlight direct irradiation sperm and ovum.
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| CN103931525B (en) * | 2014-04-09 | 2016-01-20 | 新疆维吾尔自治区水产科学研究所 | The artificial propagation of the naked skin-carp meat in Xinjiang and fry rearing method |
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| CN110036955A (en) * | 2019-05-31 | 2019-07-23 | 内江师范学院 | A kind of colored * and lip * first familiar generation seed propagation method |
| CN111264424B (en) * | 2020-02-27 | 2021-08-31 | 中国水产科学研究院珠江水产研究所 | Method for breeding snakehead fishes by utilizing artificial insemination and hatching barrel |
| CN114403057A (en) * | 2022-02-14 | 2022-04-29 | 四川省农业科学院水产研究所 | Leiocassis longirostris breeding method |
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| CN102783442A (en) * | 2012-08-23 | 2012-11-21 | 甘肃省水产研究所 | Artificial breeding method of Gymnodiptychus pachycheilus |
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Inventor after: Lin Jue Inventor after: Li Jun Inventor after: Zhou Jian Inventor after: Li Hua Inventor after: Gong Quan Inventor after: Shen Danzhou Inventor after: Du Jun Inventor after: Liu Chao Inventor before: Zhou Jian Inventor before: Lai Jiansheng Inventor before: Zhao Gang Inventor before: Li Hua Inventor before: Gong Quan Inventor before: Shen Danzhou Inventor before: Du Jun |
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