CN104255608B - Artificial spawning method for thick-lipped nude hemibarbus labeo - Google Patents
Artificial spawning method for thick-lipped nude hemibarbus labeo Download PDFInfo
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- CN104255608B CN104255608B CN201410502313.7A CN201410502313A CN104255608B CN 104255608 B CN104255608 B CN 104255608B CN 201410502313 A CN201410502313 A CN 201410502313A CN 104255608 B CN104255608 B CN 104255608B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses an artificial spawning method for thick-lipped nude hemibarbus labeo, and belongs to the technical field of artificial fish breeding. The method includes the steps: (1) preparing a fish domestication pond; (2) selecting parent fishes and performing disinfection; (3) performing artificial domestication; (4) breeding the fishes before spawning; (5) performing artificial spawning. Spawning rate can be remarkably increased, and a fine foundation is provided for further in-depth study and culture of the thick-lipped nude hemibarbus labeo.
Description
Technical field
The invention belongs to artificial propagation of fish technical field, more particularly to a kind of thickness lip naked skin-carp meat artificial induced spawning side
Method.
Background technology
The naked skin-carp meat of thick lip is commonly called as parmelia saxatilis fish, weight mouth fish, belongs to Cypriniformes, Cyprinidae, Schizothoracinae, naked skin-carp meat belong to.Dwell
Occupy in the broad valley river of plateau, especially delayed with river bend more at water, often live in gravel bottom section.Main food aquatic insect, swim dynamic
Thing and canebreak and algae etc..Bred in 4 Mays, growth is relatively slow, 9 age fish length only 400 millimeter.Domestic point
Cloth scope is substantially that west from common storehouse mountain range, Mount Min mountain range is supported in east North gets the Mountain Ranges of Tian Shan Mountains and Qilian Mountains, south to the Himalaya
And the Yunnan-Guizhou Plateau, Qinghai-Tibet Platean is the distributed areas of their concentrations, and Gansu is distributed in Lanzhou Huanghe to Maqu section, the Taohe River (Min
County is to Lintao).Although the naked skin-carp meat poor growth of thick lip, its fine and tender taste, is famous famous and precious fish, in Gansu fish crop
In occupy certain proportion, economic worth is higher.Unordered nothing due to the four seasons continual overfishing for many years, hydraulic engineering
Degree exploitation, water pollution and drought, destroy fish natural propagation, life area and its living environment, lead to stock number
It is greatly reduced, endangered.
For thick this fingerling resource in imminent danger of the naked skin-carp meat of lip of protection it is necessary to carry out artificial propagation research.Before artificial propagation
Will clearly thick lip naked skin-carp meat reproductive biology basis, differentiate its sex and development characteristics.The naked skin-carp meat gonad development of thick lip, one-tenth
Ripe and output process has strict periodicity.According to six phase of fish gonad development classification observation analysis, V phase was sexal maturity
Fish.
Patent of invention CN 103931525 A discloses a kind of artificial propagation and the fry rearing method of the naked skin-carp meat in Xinjiang, bag
Include following steps：1) collect parent population 2) parent population sterilization raises and train 3) inspection 4 of parent population) parent population is antenatal stimulates 5) artificial induced spawning 6) artificial
Adopt ovum fertilization 7) artificial incubation 8) fry rearing.This invention be directed to Xinjiang region arid and semi-arid weather half Lentic hatching method and
Seedling method, has been effectively accomplished the purpose of cultivation economized.Patent of invention CN 103444611 A discloses a kind of thickness naked heavy lip of lip
Fish artificial fecundation method, the method is carried out using semidry method artificial insemination and net cage hatching or Lentic hatching after slightly raising and train cultivation
Artificial propagation.Patent of invention CN 102783442 B discloses a kind of thickness lip naked skin-carp meat artificial fecundation method, and the method selected for V phase
Parent population ovum artificial collection semen collection, after seminal fluid and ovum are fully contacted add isotonic solution, then plus clear water stir evenly, complete inseminate, put into
Incubator is hatched.
Though said method has the advantages that individual insemination rate and incubation rate are higher, there is the extremely low defect of spawning rate.Parent population
Derive from wild, due to negligible amounts, the artificial domestication degree of nurturing is relatively low, and when gill net is fished for, the factor such as easily injured, leads to it
Physiological dysfunction or forfeiture, medicine of promoting the sexual maturity cannot be worked by normal physiological routes, leads to the rate of promoting the sexual maturity low, along with thick lip
The naked skin-carp meat duration in sexual cycle is longer, poor synchronization, and the V phase parent population quantity therefore finally meeting condition is few, even if individual
Body fertility rate and hatchability is high again, nor solves the problems, such as that this fingerling resource is in imminent danger, can only play the effect that slows down of very little, cultivation
The practice significance producing is little, not to mention large-scale cultivation.
Thus, under conditions of the insemination of thick lip naked skin-carp meat and hatching technique are more ripe, inject medicine of promoting the sexual maturity and carry out manually
The step hastened parturition becomes bottleneck step, is the restrictive condition of large-scale cultivation.
Patent of invention CN 103875567 A discloses a kind of induced spawning method of silver dragon fish, and the method uses before hastening parturition
Anaesthetic wintermin, yellow Jackets and urethane soak parent population, and your relative fish injects, after being in twilight-sleep state, medicine of promoting the sexual maturity, can
Improve spawning rate.Patent of invention CN 103222436 B discloses a kind of artificial propagation method of esox reichertii, and the method is before hastening parturition
Using 5% Phenoxyethanol anesthesia liquid, your relative fish is in the state gently stirring fish body no longer struggle, can improve spawning rate.But with
The condition such as the property of medicine of upper chemical classes anaesthetic and drug concentration is wayward, and dosage is slightly larger to be caused to be poisoned or produce other not
The parent population of the pre- insemination of foreseeable impact, especially sexal maturity, then injected chemical class promotes the sexual maturity medicine, left drug produces to it
Seminal fluid and ovum are likely to result in certain adverse effect, so that impact insemination rate, incubation rate, survival rate and fry quality.
Content of the invention
The technical problem to be solved is directed to the problem that thick lip naked skin-carp meat artificial induced spawning efficiency is low, effect is poor,
There is provided a kind of thickness lip naked skin-carp meat induced spawning method, the method significantly improves spawning rate, the rational modification thickness naked skin-carp meat of lip is urged
Produce propagation steps, further investigation further and large-scale farming for the naked skin-carp meat of thick lip provide good basis.
To achieve these goals, the present invention employs the following technical solutions and is achieved：
A kind of thickness lip naked skin-carp meat induced spawning method, comprises the following steps：
(1) domestication fishpond prepares
Early April, the sand-gravel geology domestication fishpond in natural imitation river course is separated into 9 not isometric little Chi,
Numbering A～I from big to small, little Chi are equipped with into discharge outlet, water filling mid-April and sterilization；
(2) selecting and purchasing parent population and sterilization
In late April to late May, fish for the natural river course wild thickness naked skin-carp meat of lip at least 2 ages, to no hindering what body was good for
Wild parent population disinfection, concrete sterilization method is：The aqueous solution containing salt 3～5% and the permanganic acid of 10～15mg/L
Potassium solution presses 2:1～3 volume ratio mixes makes dipping bath liquid, parent population immersion 4～10min, oxygenation in disinfecting process,
And keep water temperature to be 8～12 DEG C；
(3) artificial domestication
1. throw in parent population：Parent population after sterilization identifies its male and female sex and cycle of gonadal development, by the development of ovary IV, VI,
The female parent population of V phase puts into A, B, C little Chi respectively, and it is little that the male parent population of testis development IV, VI, V phase puts into G, H, I respectively
Pond, flowing water speed 0.1m of each little Chi3/ h, 8～12 DEG C of water temperature；
2. with tame and docile：Identify its male and female sex by after fish sterilization high for the degree of raising and train, female puts into A, B, C little Chi, male
Put into G, H, I little Chi, carry out same sexual partner with wild parent population and tame and docile, each little Chi is 2 with taming and dociling ratio:1, raising and train density is 3～5 tails/m3；
3. tame and docile food：Feed the algae being ground into 4mm in advance, then feed the mixture pulverizing algae and mash feed, then
Feed and tame and docile food hand fit's high protein feed, and add 0.01～0.1% rhizoma Gastrodiae in tame and docile food hand fit's high protein feed
Powder, daily the upper and lower noon respectively feed 1 time；
4. draw in the net to train：Late June, every 20 days, carry out drawing in the net to train 2～4 times, erythromycin is carried out to the wounded
Smear the treatment measures of injury；
5. daily management：Observe parent population ingest, the situation of change of activity and water quality, record water temperature, molten in water daily
Oxygen, the forage volume feeding, the active situation of parent population, make regular check on fish body health situation, and observe the gonad development situation of parent population；
(4) cultivate before hastening parturition
1. throw in parent population：Early Febuary next year, raising and train in A, G pond is put into the D pond of sterilization, B, H pond after parent population sterilization
In raise and train parent population sterilization after put into the E pond of sterilization, in C, I pond raise and train parent population sterilization after put into the F pond of sterilization, D, E,
The sex ration in each pond of F is 3:1；
2. reinforcing stimulus before hastening parturition：The flowing water speed in D, E, F pond increases to 0.2～0.4m3/ h, water temperature increases to 10～15 DEG C,
Carried out every 15 days drawing in the net to train 2～4 times, control light application time and intensity, 2 month light dark ratio for 1:2nd, intensity of illumination 60lx,
March light dark is than for 1:1st, intensity of illumination 60lx, April light dark is than for 5:3rd, intensity of illumination 80lx；
(5) artificial induced spawning
1. anaesthetize：Select the mature brood fish of gonad development V phase from D, E, F pond, be 1 by sex ration:1 pairing, using anesthesia
Medicine soaks to parent population, carries out the injection of oxytocic drug when parent population is in twilight-sleep state, and described anaesthetic is sky
Fiber crops element, the Gastrodin using 0.5～1mg/L soaks parent population to be injected；
2. oxytocic drug：Using oxytocic drug be No. 3 (LRH A of Leuteinizing hormone releasing hormone analogue3), DOM
(DOM) it is used in mixed way with carp pituitary (PG), using two pin injecting methods, double injection spacing 12h～24h, raun is hastened parturition
First injection amount is LRH A35 μ g/+DOM3mg/+PG1mg/, milter is not injected, and raun is hastened parturition the second injection amount：
LRH—A37 μ g/+DOM5mg/+PG2mg/, milter dosage halves；
3. injecting method：Said medicine is dissolved in 16ml sodium chloride physiological saline according to 16 times of quantity, every tail fish
By 1ml/kg injection, after injection, put into storage pond, holding pond, keep 10～15 DEG C of water temperature, flowing water speed 0.3m3/ h, dissolved oxygen 5mg/L.
The little Chi of described numbering A～I, have during external water source can by respective enter discharge outlet naturally enter row, no secondary water
During source by little Chi between draw water to manufacture interior circulation flowing water.
Described artificial domestication step stage, it is possible that ovum and sperm lead to fishpond to be polluted, at once in each little Chi
Carve and note cleaning.
The Rhizoma Gastrodiae powder containing in described tame and docile food hand fit's high protein feed, June to October, content monthly by
0.01% to 0.1% is incremented by；In November to January, content is monthly successively decreased by 0.1% to 0.01%.
Described hasten parturition before reinforcing stimulus, can be according to the different situations of gonad development in D, E, F pond, controlling stream water speed respectively
Degree, water temperature, light application time and intensity；If parent population quantity is very little, to reduce gonad development gap as far as possible, it is made by different stimulated
Sexual cycle is close to synchronous, it is to avoid later stage artificial insemination parent population lazy weight；If parent population quantity is enough, can slightly expand sexual gland and send out
Educate gap, make its sexual cycle inconsistent by different stimulated, it is to avoid shortage of manpower during later stage artificial insemination, have little time to inseminate
Situation produce.
The invention has the beneficial effects as follows：(1) domestication fishpond is separated into little Chi, then presses male and female sex and gonad development
Cycle carries out throwing in parent population, makes management standardization and scientific, can for the otherness in sexual cycle carry out different daily managements,
Feed, hasten parturition before the work such as reinforcing stimulus, be also convenient for other subsequent operations simultaneously；(2) carry out same sexual partner to tame and docile, both can avoid fish certainly
Send out insemination, domestication degree can be improved, it is to avoid subsequent artefacts are injured when hastening parturition and inseminating, the raising to spawning rate and insemination rate has again
Actively impact；(3) add Rhizoma Gastrodiae powder to have calmness, analgesia, anticonvulsion and anti inflammatory immunity work in tame and docile food hand fit's high protein feed
With being all conducive to improving parent population domestication's degree together with drawing in the net to train；(4) the anaesthetic Gastrodin safety non-toxic using,
No adverse side effect, calm and soporific function effect is good, and can increase parent population immunocompetence, useful and harmless；(5) the present invention can show
Write and improve spawning rate, further investigation further and cultivation for the naked skin-carp meat of thick lip provide good basis.
Specific embodiment
Embodiment
A kind of thickness lip naked skin-carp meat induced spawning method, comprises the following steps：
(1) domestication fishpond prepares
On April 9th, 2012, by the sand-gravel geology domestication fishpond in natural imitation river course be separated into 9 not isometric
Little Chi, numbering A～I from big to small, little Chi are equipped with into discharge outlet, April 20 water filling and sterilization；
(2) selecting and purchasing parent population and sterilization
In April 21 to May 26, fish for the natural river course wild thickness naked skin-carp meat of lip at least 2 ages, wild to no hinder that body is good for
Parent population disinfection, concrete sterilization method is：The liquor potassic permanganate of the aqueous solution containing salt 4% and 10mg/L presses 2:3
Volume ratio mix and make dipping bath liquid, parent population soaks 8min, oxygenation in disinfecting process, and keeps water temperature to be 10 DEG C；
(3) artificial domestication
1. throw in parent population：Parent population after sterilization identifies its male and female sex and cycle of gonadal development, by the development of ovary IV, VI,
The female parent population of V phase puts into A, B, C little Chi respectively, and it is little that the male parent population of testis development IV, VI, V phase puts into G, H, I respectively
Pond, flowing water speed 0.1m of each little Chi3/ h, 8～12 DEG C of water temperature；
2. with tame and docile：Identify its male and female sex by after fish sterilization high for the degree of raising and train, female puts into A, B, C little Chi, male
Put into G, H, I little Chi, carry out same sexual partner with wild parent population and tame and docile, each little Chi is 2 with taming and dociling ratio:1, raising and train density is 4 tails/m3；
3. tame and docile food：Feed the algae being ground into 4mm in advance, then feed the mixture pulverizing algae and mash feed, then
Feed and tame and docile food hand fit's high protein feed, and add 0.01～0.1% rhizoma Gastrodiae in tame and docile food hand fit's high protein feed
Powder, daily the upper and lower noon respectively feed 1 time；
4. draw in the net to train：June 25 starts, and every 20 days, carries out drawing in the net to train 2～4 times, carries out erythromycin painting to the wounded
Smear the treatment measures of injury；
5. daily management：Observe parent population ingest, the situation of change of activity and water quality, record water temperature, molten in water daily
Oxygen, the forage volume feeding, the active situation of parent population, make regular check on fish body health situation, and observe the gonad development situation of parent population；
(4) cultivate before hastening parturition
1. throw in parent population：On 2 6th, 2013, raising and train in A, G pond is put into the D pond of sterilization, B, H after parent population sterilization
In pond raise and train parent population sterilization after put into the E pond of sterilization, in C, I pond raise and train parent population sterilization after put into the F pond of sterilization, D,
The sex ration in each pond of E, F is 3:1；
2. reinforcing stimulus before hastening parturition：The flowing water speed in D, E, F pond increases to 0.2m3/ h, water temperature increases to 10～15 DEG C, every 15
It carries out drawing in the net to train 2～4 times, controls light application time and intensity, 2 month light dark ratio for 1:2nd, intensity of illumination 60lx, March
Light dark is than for 1:1st, intensity of illumination 60lx, April light dark is than for 5:3rd, intensity of illumination 80lx；
(5) artificial induced spawning
1. anaesthetize：Select the mature brood fish of gonad development V phase from D, E, F pond, be 1 by sex ration:1 pairing, using anesthesia
Medicine soaks to parent population, carries out the injection of oxytocic drug when parent population is in twilight-sleep state, and described anaesthetic is sky
Fiber crops element, the Gastrodin using 0.7mg/L soaks parent population to be injected；
2. oxytocic drug：Using oxytocic drug be No. 3 (LRH-A of Leuteinizing hormone releasing hormone analogue3), DOM
(DOM) it is used in mixed way with carp pituitary (PG), using two pin injecting methods, double injection spacing 12h～24h, raun is hastened parturition
First injection amount is LRH-A35 μ g/+DOM3mg/+PG1mg/, milter is not injected, and raun is hastened parturition the second injection amount：
LRH—A37 μ g/+DOM5mg/+PG2mg/, milter dosage halves；
3. injecting method：Said medicine is dissolved in 16ml sodium chloride physiological saline according to 16 times of quantity, every tail fish
By 1ml/kg injection, after injection, put into storage pond, holding pond, keep 10～15 DEG C of water temperature, flowing water speed 0.3m3/ h, dissolved oxygen 5mg/L.
Above-described embodiment is further illustrating that the present invention is made, but this should not be interpreted as the above-mentioned theme of the present invention
Scope is only limitted to above-described embodiment, all belongs to the scope of the present invention based on the technology that the above is realized.
Claims (4)
1. a kind of thickness lip naked skin-carp meat induced spawning method it is characterised in that：Comprise the following steps：
(1) domestication fishpond prepares
Early April, the sand-gravel geology domestication fishpond in natural imitation river course is separated into 9 not isometric little Chi, from big
To little numbering A～I, little Chi is equipped with into discharge outlet, water filling mid-April and sterilization；
(2) selecting and purchasing parent population and sterilization
In late April to late May, fish for the natural river course wild thickness naked skin-carp meat of lip at least 2 ages, wild to no hinder that body is good for
Parent population disinfection, concrete sterilization method is：The potassium permanganate of the aqueous solution containing salt 3～5% and 10～15mg/L is molten
Liquid presses 2:1～3 volume ratio mixes makes dipping bath liquid, and parent population soaks 4～10min, oxygenation in disinfecting process, and protects
Water holding temperature is 8～12 DEG C；
(3) artificial domestication
1. throw in parent population：Parent population after sterilization identifies its male and female sex and cycle of gonadal development, by the development of ovary IV, VI, V phase
Female parent population put into A, B, C little Chi respectively, the male parent population of testis development IV, VI, V phase puts into G, H, I little Chi, respectively respectively
Flowing water speed 0.1m of little Chi3/ h, 8～12 DEG C of water temperature；
2. with tame and docile：Identify its male and female sex by after fish sterilization high for the degree of raising and train, female puts into A, B, C little Chi, and male puts into
G, H, I little Chi, carries out same sexual partner with wild parent population and tames and dociles, and each little Chi is the high fish of the degree of raising and train with taming and dociling ratio：Wild parent population=2:
1, raising and train density is 3～5 tails/m3；
3. tame and docile food：Feed the algae being ground into 4mm in advance, then feed the mixture pulverizing algae and mash feed, then feed
Tame and docile food hand fit's high protein feed, and add 0.01～0.1% Rhizoma Gastrodiae powder in tame and docile food hand fit's high protein feed, often
In the sky, afternoon respectively feeds 1 time；
4. draw in the net to train：Late June, every 20 days, carry out drawing in the net to train 2～4 times, erythromycin is carried out to the wounded and smears
The treatment measures of injury；
5. daily management：Observe parent population ingest, the situation of change of activity and water quality, record water temperature, the dissolved oxygen in water, throwing daily
The forage volume fed, the active situation of parent population, make regular check on fish body health situation, and observe the gonad development situation of parent population；
(4) cultivate before hastening parturition
1. throw in parent population：Early Febuary next year, raising and train in A, G pond is put into the D pond of sterilization after parent population sterilization, in B, H pond
Put into the E pond of sterilization after raising and train parent population sterilization, in C, I pond raise and train parent population sterilization after put into the F pond of sterilization, D, E, F are each
The sex ration in pond is 3:1；
2. reinforcing stimulus before hastening parturition：The flowing water speed in D, E, F pond increases to 0.2～0.4m3/ h, water temperature increases to 10～15 DEG C, every 15
It carries out drawing in the net to train 2～4 times, controls light application time and intensity, 2 month light dark ratio for 1:2nd, intensity of illumination 60lx, March
Light dark is than for 1:1st, intensity of illumination 60lx, April light dark is than for 5:3rd, intensity of illumination 80lx；
(5) artificial induced spawning
1. anaesthetize：Select the mature brood fish of gonad development V phase from D, E, F pond, be 1 by sex ration:1 pairing, using anaesthetic
Parent population is soaked, carries out the injection of oxytocic drug when parent population is in twilight-sleep state, described anaesthetic is Gastrodin,
Gastrodin using 0.5～1mg/L soaks parent population to be injected；
2. oxytocic drug：Using oxytocic drug be No. 3 (LRH A of Leuteinizing hormone releasing hormone analogue3), DOM (DOM) and
Carp pituitary (PG) is used in mixed way, using two pin injecting methods, double injection spacing 12h～24h, and raun is hastened parturition the first injection
Measure as LRH A35 μ g/+DOM3mg/+PG1mg/, milter is not injected, and raun is hastened parturition the second injection amount：LRH—A37μg/
+ DOM5mg/+PG2mg/, milter dosage halves；
3. injecting method：Said medicine is dissolved in 16ml sodium chloride physiological saline according to 16 times of quantity, every tail fish is pressed
1ml/kg injects, and puts into storage pond, holding pond after injection, keeps 10～15 DEG C of water temperature, flowing water speed 0.3m3/ h, dissolved oxygen 5mg/L.
2. as claimed in claim 1 a kind of thickness lip naked skin-carp meat induced spawning method it is characterised in that：Described numbering A～I's
Little Chi, have during external water source can by respective enter discharge outlet naturally enter during row, no external water source by little Chi between draw water to make
Make interior circulation flowing water.
3. as claimed in claim 1 a kind of thickness lip naked skin-carp meat induced spawning method it is characterised in that：Described tame and docile anthropophagy's work
The Rhizoma Gastrodiae powder containing in cooperation high protein feed, in June to October, content is monthly incremented by by 0.01% to 0.1%；November is to 1
In month, content is monthly successively decreased by 0.1% to 0.01%.
4. as claimed in claim 1 a kind of thickness lip naked skin-carp meat induced spawning method it is characterised in that：Described hasten parturition before strong
Change and stimulate, according to the different situations of gonad development in D, E, F pond, controlling stream water speed, water temperature, light application time and intensity respectively；
If parent population quantity is very little, to reduce gonad development gap as far as possible, its sexual cycle is made close to synchronous by different stimulated；If parent population number
Amount is enough, slightly pulls open gonad development gap, makes its sexual cycle inconsistent by different stimulated.
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| CN104839069B (en) * | 2015-06-09 | 2017-06-16 | 广东海洋大学 | A kind of method for obtaining batch Duo Lin Xi parent populations |
| CN105941221A (en) * | 2016-05-16 | 2016-09-21 | 塔里木大学 | Reinforced cultivation method of parent fish of schizothorax biddulphi |
| CN106489798B (en) * | 2016-10-27 | 2019-08-30 | 武汉中科瑞华生态科技股份有限公司 | A kind of middle Warsaw loach artificial fecundation method |
| CN107950443A (en) * | 2017-12-29 | 2018-04-24 | 西南大学 | A kind of method for improving perch carp postpartum parent population survival rate |
| CN114375879B (en) * | 2021-12-29 | 2023-07-18 | 广东省鳇鲸海洋生物科技有限公司 | Cultivation method for promoting gonad development of parent yellow lip fish |
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| CN102783442A (en) * | 2012-08-23 | 2012-11-21 | 甘肃省水产研究所 | Artificial breeding method of Gymnodiptychus pachycheilus |
| CN103222436A (en) * | 2013-05-15 | 2013-07-31 | 中国水产科学研究院黑龙江水产研究所 | Artificial propagation method of esox reichertii |
| CN103444611A (en) * | 2013-09-29 | 2013-12-18 | 四川省农业科学院水产研究所 | Artificial propagation method of Gymnodiptychus pachycheilus |
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| CN103931525A (en) * | 2014-04-09 | 2014-07-23 | 新疆维吾尔自治区水产科学研究所 | Artificial reproduction and fry breeding method for Gymnodiptychus dybowskii |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102783442A (en) * | 2012-08-23 | 2012-11-21 | 甘肃省水产研究所 | Artificial breeding method of Gymnodiptychus pachycheilus |
| CN103222436A (en) * | 2013-05-15 | 2013-07-31 | 中国水产科学研究院黑龙江水产研究所 | Artificial propagation method of esox reichertii |
| CN103444611A (en) * | 2013-09-29 | 2013-12-18 | 四川省农业科学院水产研究所 | Artificial propagation method of Gymnodiptychus pachycheilus |
| CN103875567A (en) * | 2014-03-21 | 2014-06-25 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Artificial spawning method for osteoglossum bicirrhosum |
| CN103931525A (en) * | 2014-04-09 | 2014-07-23 | 新疆维吾尔自治区水产科学研究所 | Artificial reproduction and fry breeding method for Gymnodiptychus dybowskii |
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