CN102106279B - Hybrid seedlings-cultivating method for improving growth traits of siniperca scherzeri - Google Patents
Hybrid seedlings-cultivating method for improving growth traits of siniperca scherzeri Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 36
- 241000318729 Siniperca scherzeri Species 0.000 title abstract 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000005556 hormone Substances 0.000 claims abstract description 16
- 229940088597 hormone Drugs 0.000 claims abstract description 16
- 230000009027 insemination Effects 0.000 claims abstract description 12
- 241000251468 Actinopterygii Species 0.000 claims abstract description 11
- 230000012447 hatching Effects 0.000 claims abstract description 9
- 241000404975 Synchiropus splendidus Species 0.000 claims description 55
- 230000008774 maternal effect Effects 0.000 claims description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 238000002347 injection Methods 0.000 claims description 11
- 239000007924 injection Substances 0.000 claims description 11
- 239000004459 forage Substances 0.000 claims description 8
- 210000000582 semen Anatomy 0.000 claims description 8
- 102000002322 Egg Proteins Human genes 0.000 claims description 7
- 108010000912 Egg Proteins Proteins 0.000 claims description 7
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 7
- 238000009396 hybridization Methods 0.000 claims description 7
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 claims description 7
- 239000011976 maleic acid Substances 0.000 claims description 7
- 210000004681 ovum Anatomy 0.000 claims description 7
- 239000003488 releasing hormone Substances 0.000 claims description 7
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 7
- 239000004568 cement Substances 0.000 claims description 6
- 238000009402 cross-breeding Methods 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- 230000037396 body weight Effects 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 230000011218 segmentation Effects 0.000 claims description 4
- 230000001186 cumulative effect Effects 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000003643 water by type Substances 0.000 claims description 3
- 230000032696 parturition Effects 0.000 abstract description 14
- 238000009395 breeding Methods 0.000 abstract description 10
- 230000001488 breeding effect Effects 0.000 abstract description 10
- 230000001360 synchronised effect Effects 0.000 abstract description 6
- 230000004083 survival effect Effects 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 241000264847 Siniperca chuatsi Species 0.000 abstract 1
- 230000000798 anti-retroviral effect Effects 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
- 238000012856 packing Methods 0.000 abstract 1
- 230000002035 prolonged effect Effects 0.000 abstract 1
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- 230000012173 estrus Effects 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- 241000594011 Leuciscus leuciscus Species 0.000 description 2
- 241001275890 Megalobrama amblycephala Species 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 235000021191 food habits Nutrition 0.000 description 2
- 210000002149 gonad Anatomy 0.000 description 2
- 230000002710 gonadal effect Effects 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 241000252233 Cyprinus carpio Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention belongs to the agriculture technical field necessary for human life, and relates to a hybrid seedlings-cultivating method for improving the growth traits of the siniperca scherzeri. The method comprises the following main steps of: selecting the siniperca scherzeri as the male parent and the siniperca chuatsi as the female parent, and adopting the methods for building a plastic house to overwinter, intensively cultivate and the like; hastening the parturition by the means that the exogenous hormone is synchronously injected into the male parent and the female parent for once, and packing a parent fish by a dishtowel during artificial insemination; adopting the breeding technology and measurement such as the artificial semidry inseminating, the circuit water flowing hatching and the like, so that the use ratio of the male parent is improved by 1.5-2 times; and intensifying the cultivating management in the seedling stage and the cultivating stage by a segmented cultivating mode with the palatable bait fish. The invention successfully develops a method for improving the growth traits of the siniperca scherzeri to cultivate an improved new product which is antiretroviral, disease-resistant and faster in growth speed compared with the common siniperca scherzeri. Meanwhile, the existing hybrid seedlings-cultivating method of the siniperca scherzeri is changed, the synchronous mature of the parent is guaranteed, the propagation time is prolonged, the operation is simpler and more convenient, and the production efficiency and the survival rate of the artificial propagating and the hybrid seedlings hatching are improved.
Description
Technical field
The invention belongs to the essential agricultural technology field of human lives, relate to the fish culture technology, relate in more detail utilize fish interspecific cross breeding technique to produce the method for the new varieties spot mandarin fish with obvious Morphological Features and growth vigor.
Background technology
Be to improve the growth rate of spot mandarin fish, cultivate new have obvious growth vigor, the new mandarin fish kind that degeneration-resistant, disease resistance is strong, showing major technique is crossbreeding.Its producing method for seed is as follows:
1, selects the spot mandarin fish as male parent, stick up the mouth mandarin fish as female parent;
2, the stimulation by running water method promotes its sexual maturity;
3, adopt the injection exogenous hormone to hasten parturition, and inject first male parent spot mandarin fish, inject again female parent after 2 hours and stick up the mouth mandarin fish;
4, dry method of artificial fertilization;
5, hybrid seedlings is cultivated stage by stage and is carried out conversion of food habit.
Show the spot mandarin fish and stick up mouth mandarin fish hybridizing propagation process and have following shortcoming:
1, the stimulation by running water method can not promote the synchronous maturation of these two kinds of different mandarin fish sexual glands well, so that parent's breeding period can not be more effective overlapping, simultaneously, does not satisfy the upper early numerous demand of producing;
2, induced spawning method is complicated, and the injection in 2 hours of Parent interval and at twice injection have increased the stress reaction that manual operation causes parent population, is unfavorable for hastening parturition and parent's Recovery;
3, dry method of artificial fertilization, for being mainly derived from wild resource, the male parent spot mandarin fish of few people worker's cultivation, the availability of sperm is low, causes the serious waste of resource;
4, to the survival rate that conversion of food habit has reduced seed of carrying out of cross hybrid seedling.
For cultured fishes, improving fish growth speed, strengthening disease-resistant, resistance is the top priority in the breeding work, therefore cultivates on the basis of new varieties in hybridization, and in addition the selection breeding of science is conducive to the raising of breeding work effect.Produce the productivity that should take full advantage of the parent for adapting to large-scale seed simultaneously, the step that simplifies the operation is enhanced productivity.
Summary of the invention
The object of the invention is to the defective for the prior art existence, a kind of resource that takes full advantage of is provided, more effectively improve the new cross breeding method of spot mandarin fish growth traits.
The cross breeding method of improvement spot mandarin fish growth traits of the present invention mainly comprises the steps: to stick up the mouth mandarin fish and does maternal parent population, and wild spot mandarin fish carries out artificial induced spawning, insemination and seedling stage and cultivates as the male parent parent population.Each step is as follows specifically:
(1) parent population is selected and promotes sexual gland ripe synchronously:
Select physique stalwartness, fast growth, 2 ages above original seeds to stick up the mouth mandarin fish and do maternal parent population, the wild spot mandarin fish more than 1 age is as the male parent parent population.Breeding is built plastic tunnel in winter the previous year, improves temperature of cultivation, and hot housing can be carried out the cycle of gonadal development that female parent sticks up mouth mandarin fish and male parent spot mandarin fish synchronously, thereby guarantees the effectively overlapping of two kinds of mandarin fish breeding periods, the hybridization between being conducive to kind.Simultaneously the method sexual gland of making parent population is more ripe than local normal climate about 1 month in advance, can carry out morning numerous.
(2) ovum is adopted in artificial induced spawning and semen collection:
Water temperature is 20 ℃-30 ℃, and the Zhou Shuiwen temperature difference is carried out artificial induced spawning about 2 ℃.Take disposable Parent to inject synchronously the exogenous hormone method and hasten parturition, maternal parent population is injected exogenous hormone according to following consumption: every kilogram of injection of fish body weight 15-20ug luteotropin releasing hormone d-ala analog (LRH-A), 1200-1500IU human chorionic gonadtropin (HCG) and 5-8mg maleic acid DOM (DOM); The male parent parent population is injected exogenous hormone according to following consumption: every kilogram of fish body weight injection 10ug luteotropin releasing hormone d-ala analog, 750IU human chorionic gonadtropin and 4mg maleic acid DOM.The living environment temperature of parent population is 20 ℃-30 ℃ before and after the artificial induced spawning, day and night temperature 3-5 ℃.Maternal parent population and male parent parent population picked up in 24-28 behind the artificial induced spawning hour and carry out ovum artificial collection and semen collection.Semen collection or adopt dry towel parcel parent population when adopting ovum reduces the stress reaction that manual operation causes parent population.
(3) adopt semi-dry process artificial insemination:
Artificial insemination is that male parent spot mandarin fish sperm is extruded, male parent spot mandarin fish sperm concentration (2.75 * 10
10Individual/ml) approximately be to stick up mouth mandarin fish milter sperm concentration (0.59 * 10
10Individual/ml) 4.7 times.During artificial insemination, first male parent spot mandarin fish sperm is extruded, adding an amount of 0.8-0.9%(w/v) sodium chloride solution dilutes, pour in the fish-egg again and stir, add a small amount of clear water and stir a little, left standstill 1-2 minute, clean for several times with clear water, put into the circuit hatching, every cube of water body enters ten thousand of fertilized egg 2-3; Maternal quantity and the preferred 3-4:1 of quantity of male parent.Adopt this method to carry out artificial insemination, can be so that the male parent availability improves 1.5-2 doubly.
(4) cultivate seedling stage:
Take the segmentation cultivate mode, the rupture of membranes agreeable to the taste bait fry of throwing something and feeding after 2 days, such as megalobrama amblycephala, dace seedling etc., feeding volume is 4-8 tail forage fiss/every tail hybridization mandarin fish seedling, feeding volume is cumulative with age in days.Opening changes over to the cement from circuit after 4 days and cultivates, 18 square metres of cement pit areas, and 0.8-1 meter of the depth of water is put 2000-3000 tails for every square metre.Put into the pond when reaching 1cm and carry out the cultivation of special pond, 1-2 mu of pond areas, 1.2 meters-1.5 meters of the depth of waters are put 4000 tails in a suitable place to breed for every mu, the forage fiss of the mandarin fish gross weight 5%-10% of throwing something and feeding.
The inventive method is to the Parent reinforced cultivating of puing up a shed, and guarantees the effectively overlapping of two kinds of different mandarin fish breeding periods, promotes the synchronous maturation of sexual gland, and this is the necessary condition that interspecific cross produces the first filial generation.Simultaneously, because spot mandarin fish resource rare, carry out artificial induced spawning and the dry method of artificial fertilization of biphasic injection, be unfavorable for the Recovery of spot mandarin fish and the efficient and rational use of resource.Therefore, the present invention compared with prior art has following advantage:
1, improve temperature of cultivation in the mode of building plastic tunnel, hot housing can be carried out the cycle of gonadal development that female parent sticks up mouth mandarin fish and male parent spot mandarin fish synchronously, thereby guarantees the effectively overlapping of two kinds of mandarin fish breeding periods, the hybridization between being conducive to kind.Simultaneously the method sexual gland of making parent population is more ripe than local normal climate about 1 month in advance, can carry out morning numerous, thereby take full advantage of vegetative period, cultivates large size fingerling, and the output of raising fish obtains good production effect;
2, take the synchronous shot exogenous hormone of Parent method to hasten parturition, the consumption of hormone has important effect to the spawning rate that improves parent population, adopts hormone dosage of the present invention, can reach the spawning rate of 93-96%;
3. adopt dry towel parcel parent population during artificial insemination, reduce the stress reaction that manual operation causes parent population, save time, laborsaving, help simultaneously hastening parturition and Recovery of parent;
3, adopt semi-dry process artificial insemination, make the male parent spot mandarin fish availability that is mainly derived from wild resource improve 1.5-2 doubly;
4, the segmentation in seedling stage is cultivated and the agreeable to the taste bait fry of throwing something and feeding, and has obviously improved the survival rate of cross hybrid seedling.
Embodiment
Further explain the present invention below in conjunction with embodiment, but embodiment does not do any type of restriction to the present invention.
Embodiment 1
The invention will be further described below by embodiment.
1. parent population is introduced and reinforced cultivating.
Introduce 2 ages above original seeds December and stick up mouth mandarin fish parent population as female parent, 1 age, above wild spot mandarin fish was as male parent; Raise and train and cultivate in the plastic tunnel pond of building, strengthen feeding and management, the sufficient forage fiss of throwing something and feeding.
2. artificial propagation.
1. the parent selects: begin to check the parent population maturity mid-March, at the beginning of 4 months, parent's synchronous maturing rate reaches more than 80%.Water temperature can be carried out artificial propagation more than 20 ℃ the time.Select that belly expands, soft, ovary profile obviously, the soft and whippy gonad maturity of hand belly stick up mouth mandarin fish raun as female parent.Select indent in the middle of the anus be blush, gently press belly to have the milky seminal fluid to flow out and entry namely loose spot mandarin fish milter as male parent.
2. artificial induced spawning: the parent to gonad maturity takes synchronous shot exogenous hormone method to hasten parturition, the oxytocic hormone kind has luteotropin releasing hormone d-ala analog (LRH-A), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM), maternal parent population injected dose is as shown in table 1 respectively, and male parent parent population dosage reduces by half.And female milter is injected simultaneously.Parent population after the injection is placed in the circuit and supports temporarily, and stimulation by running water promotes it to oestrus.Check the situation of hastening parturition after 24 hours, reach a climax when being about to lay eggs when parent population oestruses (be effect time to reach oestrus climax required time from artificial induced spawning) caught parent population immediately, carries out ovum artificial collection, semen collection.Different injection hormones and injected dose see Table 2 to the impact of parent population spawning rate during artificial induced spawning, and the spawning rate by luteotropin releasing hormone d-ala analog (LRH-A), human chorionic gonadtropin (HCG) and three kinds of hormone combinations injections of maleic acid DOM (DOM) is the highest as shown in Table 2.And under different water temperature conditions, these three kinds of hormones are injected in dosage ratio scope of the present invention, and to parent population spawning effect all good (seeing Table 2), when water temperature was lower than 20 ℃, spawning rate was relatively low, and therefore, the water temperature before and after hastening parturition preferably remains on more than 20 ℃.
The contrast of table 1 hormon and dosage artificial induced spawning effect
| Time | Water temperature | The dosage of hastening parturition | The mantissa of hastening parturition | The mantissa of laying eggs | Spawning rate |
| 2008 | 25—27℃ | LHR-A15ug/kg+HCG1500IU/kg+DOM6mg/kg | ? 28 | 27 | 96% |
| 2008 | 25—27℃ | LHR-A10ug /kg+HCG1500IU/kg | 8 | 7 | 88％ |
| 2008 | 25—27℃ | LHR-A15ug/kg+HCG1800IU/kg | 9 | 7 | 78% |
| 2008 | 25—27℃ | PG2mg/kg+ LHR-A6ug /kg+HCG1000IU/kg | 8 | 6 | 75％ |
* luteotropin releasing hormone d-ala analog (LHR-A), human chorionic gonadtropin (HCG) and maleic acid DOM (DOM), PG(carp brain hypophysis).
The artificial induced spawning effect of the different injected dose of table 2, different water temperature parent populations
| Time | Water temperature | The dosage of hastening parturition | The mantissa of hastening parturition | The mantissa of laying eggs | Spawning rate |
| 2008 | 25—27℃ | LHR-A15ug/kg+HCG1500IU/kg+DOM6mg/kg | ? 28 | 27 | 96% |
| 2009 | 22—24℃ | LHR-A18ug/kg+HCG1200IU/kg+DOM8mg/kg | 15 | 14 | 93% |
| 2010 | 25—27℃ | LHR-A20ug/kg+HCG1200IU/kg+DOM5mg/kg | 21 | 20 | 95% |
| 2010 | 19—20℃ | LHR-A20ug/kg+HCG1500IU/kg+DOM5mg/kg | 14 | 12 | 86％ |
3. semi-dry process artificial insemination: adopt dry towel parcel parent population, first male parent spot mandarin fish sperm is extruded, add 0.85% sodium chloride solution be equivalent to 5 times of sperm volumes and dilute that (male parent spot mandarin fish sperm concentration approximately is to stick up 4.7 times of mouth mandarin fish milter sperm concentration as shown in Table 3, therefore the dilution volume is the volume of 5 times of sperms.Simultaneously, table 4 shows that the sodium chloride solution of 0.8%-0.9% concentration can not activate sperm, therefore can be used for the dilution in early stage of sperm), pour in the fish-egg again and stir, add a small amount of clear water and stir a little, left standstill 2 minutes, clean for several times with clear water, put into the circuit hatching.Be 4:1 in order to maternal quantity and the male parent quantitative proportion of adopting ovum and semen collection.
The biological property of the different mandarin fish sperms of table 3
| Kind | PH of semen | Sperm length (um) | Essence density at night (individual/ml) |
| Stick up the mouth mandarin fish | 6.79 | 31.85 | 0.59×10 10 |
| The spot mandarin fish | 7.18 | 28.75 | 2.75×10 10 |
The sodium chloride solution of table 4 variable concentrations is on the impact of activation of spermatozoa rate
| Concentration of sodium chloride solution % | Activity ratio (%) |
| 0 | 70 |
| 0.1 | 70 |
| 0.3 | 65 |
| 0.5 | 52.5 |
| 0.6 | 40 |
| 0.7 | 5 |
| 0.8 | 0 |
| 0.85 | 0 |
| 0.9 | 0 |
4. hatching: adopt the circuit Lotic hatching, every cube of water body enters 30,000 in fertilized egg, keeps water quality pure and fresh, is strictly on guard against that harmful animal enters, and regulates current, strengthens management.
3. cultivate seedling stage.
Take the segmentation cultivate mode, the rupture of membranes agreeable to the taste bait fry of throwing something and feeding after 2 days, such as megalobrama amblycephala, dace seedling etc., feeding volume is 4-8 tail forage fiss/every tail hybridization mandarin fish seedling, feeding volume is cumulative with age in days.Opening changes over to the cement from circuit after 4 days and cultivates, 18 square metres of cement pit areas, and 0.8 meter of the depth of water is put 3000 tails for every square metre.Put into the pond when reaching 1cm and carry out the cultivation of special pond, the pond area is 2 mu, and 1.2 meters of the depth of waters are put 4000 tails in a suitable place to breed for every mu, the forage fiss of the mandarin fish gross weight 5%-10% of throwing something and feeding, and in time adjust and replenish according to forage fiss volume residual in the fishpond at any time.Carry out the work such as water quality management and disease control.The hybrid seedlings survival rate reaches more than 87% and (sees Table 5).The body weight that cultivates hybridization mandarin fish after 6 months reaches about 500g, embodies obvious hybrid growth vigor, reaches the listing specification.
Table 5 adopts said method to carry out the technical parameter of hybrid breeding
| Time | Water temperature | The dosage of hastening parturition | Spawning rate | The insemination rate | Incubation rate | Weed survival rate |
| 2008 | 25—27℃ | LHR-A15ug/kg+HCG1500IU/kg+DOM6mg/kg | 96% | 89.8％ | 80.1% | 93％ |
| 2009 | 22—24℃ | LHR-A18ug/kg+HCG1200IU/kg+DOM8mg/kg | 93% | 86.5% | 78.6% | 87％ |
| 2010 | 25—27℃ | LHR-A20ug/kg+HCG1200IU/kg+DOM5mg/kg | 95% | 90.1％ | 83.3% | 94％ |
Claims (8)
1. cross breeding method of improveing spot mandarin fish growth traits, do maternal parent population to stick up the mouth mandarin fish, wild spot mandarin fish is as the male parent parent population, carry out artificial induced spawning, artificial insemination and seedling stage and cultivate, it is characterized in that described artificial induced spawning is that maternal parent population is injected exogenous hormone according to following consumption: every kilogram of fish body weight injection 15ug luteotropin releasing hormone d-ala analog, 1500IU human chorionic gonadtropin and 6mg maleic acid DOM; The male parent parent population is injected exogenous hormone according to following consumption: every kilogram of fish body weight injection 7.5ug luteotropin releasing hormone d-ala analog, 750IU human chorionic gonadtropin and 3mg maleic acid DOM; Described artificial insemination is that male parent spot mandarin fish sperm is extruded, adding the 0.8-0.9%(w/v be equivalent to 4-6 times of sperm volume) sodium chloride solution dilutes, the fish-egg adding that to adopt again the ovum gained wherein stirs, and leaves standstill 1-2 minute, cleans with clear water and puts into circular hatching channal again.
2. the method for claim 1 is characterized in that the living environment temperature of described artificial induced spawning front and back parent population is 20 ℃-30 ℃, day and night temperature 3-5 ℃.
3. the method for claim 1 is characterized in that described artificial induced spawning is that maternal parent population and male parent parent population are injected exogenous hormone synchronously.
4. the method for claim 1 is characterized in that behind the described artificial induced spawning 24-28 hour maternal parent population and male parent parent population being picked up and carries out ovum artificial collection and semen collection.
5. the method for claim 1, it is characterized in that described maternal parent population be 2 ages above original seed stick up the mouth mandarin fish, the male parent parent population is above wild spot mandarin fish in 1 age.
6. method as claimed in claim 5 is characterized in that described maternal parent population and male parent parent population carry out first hot housing carrying out artificial propagation winter the previous year: build plastic tunnel, improve temperature of cultivation.
7. the method for claim 1 is characterized in that described hatching employing circuit Lotic hatching, and every cube of water body enters ten thousand of fertilized egg 2-3.
8. the method for claim 1 is characterized in that cultivating and takes segmentation cultivate mode, the rupture of membranes agreeable to the taste bait fry of throwing something and feeding after 2 days described seedling stage, and feeding volume is 4-8 tail forage fiss/every tail hybridization mandarin fish seedling, and feeding volume is cumulative with age in days; Opening changes over to the cement from circuit after 4 days and cultivates, 18 square metres of cement pit areas, 0.8-1 meter of the depth of water, put 2000-3000 tails for every square metre, put into the pond when reaching 1cm and carry out the cultivation of special pond, 1-2 mu of pond areas, 1.2 meters-1.5 meters of the depth of waters, put 4000 tails in a suitable place to breed for every mu, the forage fiss of the mandarin fish gross weight 5%-10% of throwing something and feeding.
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| CN104145865A (en) * | 2014-08-18 | 2014-11-19 | 池州市秋浦特种水产开发有限公司 | Breeding method of hybrid mandarin fishes |
| CN105145419B (en) * | 2015-07-09 | 2018-02-06 | 中国科学院南海海洋研究所 | A kind of breeding method for obtaining strain line hippocampus heterosis, hybrid vigor |
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| CN105360037A (en) * | 2015-12-23 | 2016-03-02 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Siniperca scherzeri culturing method |
| CN105766732A (en) * | 2016-03-30 | 2016-07-20 | 邵侠 | Artificial reproduction method of mandarin fish |
| CN108812460A (en) * | 2018-07-16 | 2018-11-16 | 镇江水中仙渔业发展有限公司 | A kind of breeding method hybridizing mandarin fish |
| CN110419470B (en) * | 2019-08-05 | 2021-10-12 | 华中农业大学 | Indoor industrial reproduction method of mandarin fish |
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