CN104255608A - Artificial spawning method for thick-lipped hemibarbus labeo - Google Patents
Artificial spawning method for thick-lipped hemibarbus labeo Download PDFInfo
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- CN104255608A CN104255608A CN201410502313.7A CN201410502313A CN104255608A CN 104255608 A CN104255608 A CN 104255608A CN 201410502313 A CN201410502313 A CN 201410502313A CN 104255608 A CN104255608 A CN 104255608A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses an artificial spawning method for thick-lipped hemibarbus labeo, and belongs to the technical field of artificial fish breeding. The method includes the steps: (1) preparing a fish domestication pond; (2) selecting parent fishes and performing disinfection; (3) performing artificial domestication; (4) breeding the fishes before spawning; (5) performing artificial spawning. Spawning rate can be remarkably increased, and a fine foundation is provided for further in-depth study and culture of the thick-lipped hemibarbus labeo.
Description
Technical field
The invention belongs to artificial propagation of fish technical field, relate to a kind of thick lip skin-carp meat induced spawning method particularly.
Background technology
Thick lip skin-carp meat is commonly called as parmelia saxatilis fish, heavy mouth fish, belongs to Cypriniformes, Cyprinidae, Schizothoracinae, naked skin-carp meat genus.In broad valley river, perch plateau, especially delay water place with river bend more, section life at the bottom of gravel of being everlasting.Main food aquatic insect, zooplankton and canebreak and algae etc.Bred 4-May, growth is comparatively slow, 9 fish lengths in age only 400 millimeter.Domestic distribution is North gets the Mountain Ranges of Tian Shan Mountains and Qilian Mountains substantially, reaches the Himalaya in the south, from mountain range, common storehouse, Mount Min mountain range and the Yunnan-Guizhou Plateau are supported in east, Qinghai-Tibet Platean is their the most concentrated distributed areas, and Gansu is distributed in Lanzhou Huanghe to Maqu section, the Taohe River (Minxian County is to Lintao).Although thick lip skin-carp meat poor growth, its fine and tender taste, be famous famous and precious fish, in the fish crop of Gansu, occupy certain proportion, economic worth is higher.Due to the unordered immoderate exploitation of the four seasons continual overfishing, river construction for many years, water pollution and drought, destroy fish natural propagation, life area and living environment thereof, cause stock number significantly to reduce, endangered.
For protecting this fingerling resource in imminent danger of thick lip skin-carp meat, be necessary to carry out artificial propagation research.Thick lip skin-carp meat reproductive biology basis to be specified before artificial propagation, differentiate its sex and development characteristics.Thick lip skin-carp meat gonad development, maturation and output process have strict periodicity.According to fish gonad development six phase classification observation analysis, V phase was sexual maturity fish.
Patent of invention CN 103931525 A discloses artificial propagation and the fry rearing method of the naked skin-carp meat in a kind of Xinjiang, comprises the following steps: 1) collect parent population 2) parent population sterilization raises and train 3) inspection 4 of parent population) the antenatal stimulation 5 of parent population) artificial induced spawning 6) ovum artificial collection fertilization 7) artificial incubation 8) fry rearing.This invention, for half Lentic hatching method and seedling method of Xinjiang region arid and semi-arid weather, effectively reaches the object of cultivation economized.Patent of invention CN 103444611 A discloses the naked skin-carp meat artificial fecundation method of a kind of thick lip, and the method adopts semi-dry process artificial insemination and net cage hatching or Lentic hatching to carry out artificial propagation after raising and train a little and cultivating.Patent of invention CN 102783442 B discloses the naked skin-carp meat artificial fecundation method of a kind of thick lip, and the method selects the parent population ovum artificial collection semen collection of V phase, after seminal fluid fully contacts with ovum, add isotonic solution, then adds clear water and stir evenly, and completes insemination, puts into incubator box hatching.
Though said method has individual insemination rate and the higher advantage of incubation rate, there is the defect that spawning rate is extremely low.Parent population all derives from wild, due to negligible amounts, the artificial domestication degree of nurturing is lower, the easy factor such as injured when gill net is fished for, cause its physiological dysfunction or forfeiture, medicine of promoting the sexual maturity cannot be worked by normal physiological routes, cause the rate of promoting the sexual maturity low, it is longer to add the thick lip skin-carp meat duration in sexual cycle, poor synchronization, therefore the V phase parent population quantity finally satisfied condition is few, even if individual fertility rate and hatchability is high again, the problem that this fingerling resource is in imminent danger can not be solved, can only play and very little slow down effect, the practice significance of breeding production is little, say nothing of large-scale cultivation.
Thus, under the insemination of thick lip skin-carp meat and the more ripe condition of hatching technique, injecting the step that medicine of promoting the sexual maturity carries out artificial induced spawning becomes bottleneck step, is the restrictive condition of large-scale cultivation.
Patent of invention CN 103875567 A discloses the induced spawning method of the imperial fish of a kind of silver, and the method uses anaesthetic wintermin, yellow Jackets and urethane to soak parent population before hastening parturition, and injects medicine of promoting the sexual maturity, can improve spawning rate after your relative fish is in twilight-sleep state.Patent of invention CN 103222436 B discloses a kind of artificial propagation method of esox reichertii, and the method uses 5% Phenoxyethanol anesthesia liquid before hastening parturition, and your relative fish is in the state stirred fish body gently and no longer struggle, and can improve spawning rate.But the conditions such as the property of medicine of above chemical classes anaesthetic and drug concentration are wayward, dosage slightly conference causes poisoning or produces other unpredictable impact, especially the parent population that inseminates in advance of sexual maturity, injected chemical class is promoted the sexual maturity medicine again, left drug may cause certain adverse effect to its seminal fluid produced and ovum, so that affect insemination rate, incubation rate, survival rate and fry quality.
Summary of the invention
Technical problem to be solved by this invention is low for thick lip skin-carp meat artificial induced spawning efficiency, the problem of weak effect, a kind of thick lip skin-carp meat induced spawning method is provided, the method significantly improves spawning rate, the thick lip skin-carp meat of rational modification is hastened parturition propagation steps, for the further further investigation of thick lip skin-carp meat and large-scale farming provide good basis.
To achieve these goals, the present invention is achieved by the following technical solutions:
A kind of thick lip skin-carp meat induced spawning method, comprises the following steps:
(1) tame fishpond to prepare
Early April, the sand-gravel geology domestication fishpond in natural imitation river course is separated into 9 not isometric little Chi, and numbering A from big to small ~ I, little Chi is equipped with into discharge outlet, water filling mid-April and sterilization;
(2) selecting and purchasing parent population and sterilization
In late April to late May, fish for the wild thick lip skin-carp meat of natural river course at least 2 ages, the strong wild parent population disinfection of body is hindered to nothing, concrete sterilization method is: to mix by the volume ratio of 2:1 ~ 3 containing the aqueous solution of salt 3 ~ 5% and the liquor potassic permanganate of 10 ~ 15mg/L and make dipping bath liquid, parent population soaks 4 ~ 10min, oxygenation in disinfecting process, and keep water temperature to be 8 ~ 12 DEG C;
(3) artificial domestication
1. parent population is thrown in: the parent population after sterilization identifies its male and female sex and cycle of gonadal development, the female parent population of the development of ovary IV, VI, V phase is dropped into A, B, C little Chi respectively, the male parent population of testis development IV, VI, V phase drops into G, H, I little Chi respectively, the flowing water speed 0.1m of each little Chi
3/ h, water temperature 8 ~ 12 DEG C;
2. companion tames and dociles: by its male and female sex of qualification after fish high for the degree of raising and train sterilization, female input A, B, C little Chi, and male input G, H, I little Chi, carry out same sexual partner with wild parent population and tame and docile, and each little Chi companion tames and dociles than being 2:1, and raising and train density is 3 ~ 5 tails/m
3;
3. food is tamed and dociled: throw something and feed in advance and be ground into the algae of 4mm, then throw something and feed and pulverize the mixture of algae and mash feed, throw something and feed and tame and docile food hand fit high protein feed, and add the Rhizoma Gastrodiae powder of 0.01 ~ 0.1% in tame and docile food hand fit high protein feed, every day at the upper and lower noon respectively throws something and feeds 1 time;
4. draw in the net to train: late June, every 20 days, carry out drawing in the net training 2 ~ 4 times, carry out to the wounded treatment measures that injury smeared by erythromycin;
5. daily management: observe that parent population ingests, the situation of change of movable and water quality, record every day the dissolved oxygen in water temperature, water, the forage volume of throwing something and feeding, parent population active situation, make regular check on fish body health status, and observe the gonad development situation of parent population;
(4) cultivate before hastening parturition
1. parent population is thrown in: early Febuary next year, the input D pond of having sterilized after parent population sterilization will be raised and train in A, G pond, raise and train the input E pond of having sterilized after parent population sterilization in B, H pond, in C, I pond raise and train parent population sterilization after the input F pond of having sterilized, the sex ration in each pond of D, E, F is 3:1;
2. reinforcing stimulus before hastening parturition: the flowing water speed in D, E, F pond increases to 0.2 ~ 0.4m
3/ h, water temperature increases to 10 ~ 15 DEG C, carries out drawing in the net training 2 ~ 4 times every 15 days, controlled light time and intensity, February light/dark than be 1:2, intensity of illumination 60lx, March light/secretly than being 1:1, intensity of illumination 60lx, April light/secretly than being 5:3, intensity of illumination 80lx;
(5) artificial induced spawning
1. anaesthetize: the mature brood fish selecting gonad development V phase from D, E, F pond, by sex ration be 1:1 pairing, anaesthetic is used to soak parent population, the injection of oxytocic drug is carried out when parent population is in twilight-sleep state, described anaesthetic is Gastrodin, adopts the Gastrodin of 0.5 ~ 1mg/L to soak parent population to be injected;
2. oxytocic drug: the oxytocic drug of employing is Leuteinizing hormone releasing hormone analogue No. 3 (LRH-A
3), DOM (DOM) and carp brain hypophysis (PG) used in combination, adopt two pin injecting methods, double injection spacing 12h ~ 24h, raun the first injection amount of hastening parturition is LRH-A
35 μ g/ ㎏+DOM3mg/ ㎏+PG1mg/ ㎏, milter is not injected, and raun is hastened parturition second injection amount: LRH-A
37 μ g/ ㎏+DOM5mg/ ㎏+PG2mg/ ㎏, milter dosage reduces by half;
3. injecting method: be dissolved in 16ml sodium chloride physiological saline according to the quantity of 16 times by said medicine, every tail fish presses 1ml/kg injection, puts into storage pond, holding pond after injection, keeps water temperature 10 ~ 15 DEG C, flowing water speed 0.3m
3/ h, dissolved oxygen 5mg/L.
The little Chi of described numbering A ~ I, having during external water source and naturally enters row by respective discharge outlet of entering, manufacturing Inner eycle flowing water without during external water source by drawing water between little Chi.
Described artificial domestication step stage, may occur in each little Chi that ovum and sperm cause fishpond to be polluted, and should note cleaning the moment.
The Rhizoma Gastrodiae powder contained in described tame and docile food hand fit high protein feed, June, content monthly increased progressively by 0.01% to 0.1% to October; November, content monthly successively decreased by 0.1% to 0.01% to January.
Described hasten parturition before reinforcing stimulus, can according to the different situations of gonad development in D, E, F pond, control flow check water speed, water temperature, light application time and intensity respectively; If parent population quantity very little, to reduce gonad development gap as far as possible, make its sexual cycle close to synchronous by different stimulated, avoid later stage artificial insemination parent population lazy weight; If parent population quantity is abundant, can expand gonad development gap a little, make its sexual cycle inconsistent by different stimulated, shortage of manpower when avoiding later stage artificial insemination, the situation having little time to inseminate produces.
The invention has the beneficial effects as follows: (1) domestication fishpond is separated into little Chi, input parent population is carried out again by male and female sex and cycle of gonadal development, make management standardization and scientific, can the otherness in specific aim cycle carry out different daily managements, throw something and feed, hasten parturition before the work such as reinforcing stimulus, also facilitate other subsequent operation simultaneously; (2) carry out same sexual partner to tame and docile, both can avoid the spontaneous insemination of fish, and domestication degree can have been improved again, injured when avoiding subsequent artefacts to hasten parturition and inseminate, there is positive impact to the raising of spawning rate and insemination rate; (3) in tame and docile food hand fit high protein feed, add Rhizoma Gastrodiae powder and have calmness, analgesia, anticonvulsion and antiinflammatory immunity function, be all conducive to improving parent population domestication degree together with drawing in the net to train; (4) the anaesthetic Gastrodin safety non-toxic used, without adverse side effect, calm and soporific function is effective, and parent population immunocompetence can be increased, useful and harmless; (5) the present invention can significantly improve spawning rate, for the further further investigation of thick lip skin-carp meat and cultivation provide good basis.
Embodiment
Embodiment
A kind of thick lip skin-carp meat induced spawning method, comprises the following steps:
(1) tame fishpond to prepare
On April 9th, 2012, the sand-gravel geology domestication fishpond in natural imitation river course is separated into 9 not isometric little Chi, and numbering A from big to small ~ I, little Chi is equipped with into discharge outlet, water filling on April 20 and sterilization;
(2) selecting and purchasing parent population and sterilization
In 21 to May 26 in April, fish for the wild thick lip skin-carp meat of natural river course at least 2 ages, the strong wild parent population disinfection of body is hindered to nothing, concrete sterilization method is: to mix by the volume ratio of 2:3 containing the aqueous solution of salt 4% and the liquor potassic permanganate of 10mg/L and make dipping bath liquid, parent population soaks 8min, oxygenation in disinfecting process, and keep water temperature to be 10 DEG C;
(3) artificial domestication
1. parent population is thrown in: the parent population after sterilization identifies its male and female sex and cycle of gonadal development, the female parent population of the development of ovary IV, VI, V phase is dropped into A, B, C little Chi respectively, the male parent population of testis development IV, VI, V phase drops into G, H, I little Chi respectively, the flowing water speed 0.1m of each little Chi
3/ h, water temperature 8 ~ 12 DEG C;
2. companion tames and dociles: by its male and female sex of qualification after fish high for the degree of raising and train sterilization, female input A, B, C little Chi, and male input G, H, I little Chi, carry out same sexual partner with wild parent population and tame and docile, and each little Chi companion tames and dociles than being 2:1, and raising and train density is 4 tails/m
3;
3. food is tamed and dociled: throw something and feed in advance and be ground into the algae of 4mm, then throw something and feed and pulverize the mixture of algae and mash feed, throw something and feed and tame and docile food hand fit high protein feed, and add the Rhizoma Gastrodiae powder of 0.01 ~ 0.1% in tame and docile food hand fit high protein feed, every day at the upper and lower noon respectively throws something and feeds 1 time;
4. draw in the net to train: June 25 starts, and every 20 days, carries out drawing in the net training 2 ~ 4 times, carries out to the wounded treatment measures that injury smeared by erythromycin;
5. daily management: observe that parent population ingests, the situation of change of movable and water quality, record every day the dissolved oxygen in water temperature, water, the forage volume of throwing something and feeding, parent population active situation, make regular check on fish body health status, and observe the gonad development situation of parent population;
(4) cultivate before hastening parturition
1. parent population is thrown in: on February 6th, 2013, the input D pond of having sterilized after parent population sterilization will be raised and train in A, G pond, raise and train the input E pond of having sterilized after parent population sterilization in B, H pond, in C, I pond raise and train parent population sterilization after the input F pond of having sterilized, the sex ration in each pond of D, E, F is 3:1;
2. reinforcing stimulus before hastening parturition: the flowing water speed in D, E, F pond increases to 0.2m
3/ h, water temperature increases to 10 ~ 15 DEG C, carries out drawing in the net training 2 ~ 4 times every 15 days, controlled light time and intensity, February light/dark than be 1:2, intensity of illumination 60lx, March light/secretly than being 1:1, intensity of illumination 60lx, April light/secretly than being 5:3, intensity of illumination 80lx;
(5) artificial induced spawning
1. anaesthetize: the mature brood fish selecting gonad development V phase from D, E, F pond, by sex ration be 1:1 pairing, anaesthetic is used to soak parent population, the injection of oxytocic drug is carried out when parent population is in twilight-sleep state, described anaesthetic is Gastrodin, adopts the Gastrodin of 0.7mg/L to soak parent population to be injected;
2. oxytocic drug: the oxytocic drug of employing is Leuteinizing hormone releasing hormone analogue No. 3 (LRH-A
3), DOM (DOM) and carp brain hypophysis (PG) used in combination, adopt two pin injecting methods, double injection spacing 12h ~ 24h, raun the first injection amount of hastening parturition is LRH-A
35 μ g/ ㎏+DOM3mg/ ㎏+PG1mg/ ㎏, milter is not injected, and raun is hastened parturition second injection amount: LRH-A
37 μ g/ ㎏+DOM5mg/ ㎏+PG2mg/ ㎏, milter dosage reduces by half;
3. injecting method: be dissolved in 16ml sodium chloride physiological saline according to the quantity of 16 times by said medicine, every tail fish presses 1ml/kg injection, puts into storage pond, holding pond after injection, keeps water temperature 10 ~ 15 DEG C, flowing water speed 0.3m
3/ h, dissolved oxygen 5mg/L.
Above-described embodiment is further illustrating of making the present invention, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to above-described embodiment, and all technology realized based on foregoing all belong to scope of the present invention.
Claims (4)
1. a thick lip skin-carp meat induced spawning method, is characterized in that: comprise the following steps:
(1) tame fishpond to prepare
Early April, the sand-gravel geology domestication fishpond in natural imitation river course is separated into 9 not isometric little Chi, and numbering A from big to small ~ I, little Chi is equipped with into discharge outlet, water filling mid-April and sterilization;
(2) selecting and purchasing parent population and sterilization
In late April to late May, fish for the wild thick lip skin-carp meat of natural river course at least 2 ages, the strong wild parent population disinfection of body is hindered to nothing, concrete sterilization method is: to mix by the volume ratio of 2:1 ~ 3 containing the aqueous solution of salt 3 ~ 5% and the liquor potassic permanganate of 10 ~ 15mg/L and make dipping bath liquid, parent population soaks 4 ~ 10min, oxygenation in disinfecting process, and keep water temperature to be 8 ~ 12 DEG C;
(3) artificial domestication
1. parent population is thrown in: the parent population after sterilization identifies its male and female sex and cycle of gonadal development, the female parent population of the development of ovary IV, VI, V phase is dropped into A, B, C little Chi respectively, the male parent population of testis development IV, VI, V phase drops into G, H, I little Chi respectively, the flowing water speed 0.1m of each little Chi
3/ h, water temperature 8 ~ 12 DEG C;
2. companion tames and dociles: by its male and female sex of qualification after fish high for the degree of raising and train sterilization, female input A, B, C little Chi, and male input G, H, I little Chi, carry out same sexual partner with wild parent population and tame and docile, and each little Chi companion tames and dociles than being 2:1, and raising and train density is 3 ~ 5 tails/m
3;
3. food is tamed and dociled: throw something and feed in advance and be ground into the algae of 4mm, then throw something and feed and pulverize the mixture of algae and mash feed, throw something and feed and tame and docile food hand fit high protein feed, and add the Rhizoma Gastrodiae powder of 0.01 ~ 0.1% in tame and docile food hand fit high protein feed, every day at the upper and lower noon respectively throws something and feeds 1 time;
4. draw in the net to train: late June, every 20 days, carry out drawing in the net training 2 ~ 4 times, carry out to the wounded treatment measures that injury smeared by erythromycin;
5. daily management: observe that parent population ingests, the situation of change of movable and water quality, record every day the dissolved oxygen in water temperature, water, the forage volume of throwing something and feeding, parent population active situation, make regular check on fish body health status, and observe the gonad development situation of parent population;
(4) cultivate before hastening parturition
1. parent population is thrown in: early Febuary next year, the input D pond of having sterilized after parent population sterilization will be raised and train in A, G pond, raise and train the input E pond of having sterilized after parent population sterilization in B, H pond, in C, I pond raise and train parent population sterilization after the input F pond of having sterilized, the sex ration in each pond of D, E, F is 3:1;
2. reinforcing stimulus before hastening parturition: the flowing water speed in D, E, F pond increases to 0.2 ~ 0.4m
3/ h, water temperature increases to 10 ~ 15 DEG C, carries out drawing in the net training 2 ~ 4 times every 15 days, controlled light time and intensity, February light/dark than be 1:2, intensity of illumination 60lx, March light/secretly than being 1:1, intensity of illumination 60lx, April light/secretly than being 5:3, intensity of illumination 80lx;
(5) artificial induced spawning
1. anaesthetize: the mature brood fish selecting gonad development V phase from D, E, F pond, by sex ration be 1:1 pairing, anaesthetic is used to soak parent population, the injection of oxytocic drug is carried out when parent population is in twilight-sleep state, described anaesthetic is Gastrodin, adopts the Gastrodin of 0.5 ~ 1mg/L to soak parent population to be injected;
2. oxytocic drug: the oxytocic drug of employing is Leuteinizing hormone releasing hormone analogue No. 3 (LRH-A
3), DOM (DOM) and carp brain hypophysis (PG) used in combination, adopt two pin injecting methods, double injection spacing 12h ~ 24h, raun the first injection amount of hastening parturition is LRH-A
35 μ g/ ㎏+DOM3mg/ ㎏+PG1mg/ ㎏, milter is not injected, and raun is hastened parturition second injection amount: LRH-A
37 μ g/ ㎏+DOM5mg/ ㎏+PG2mg/ ㎏, milter dosage reduces by half;
3. injecting method: be dissolved in 16ml sodium chloride physiological saline according to the quantity of 16 times by said medicine, every tail fish presses 1ml/kg injection, puts into storage pond, holding pond after injection, keeps water temperature 10 ~ 15 DEG C, flowing water speed 0.3m
3/ h, dissolved oxygen 5mg/L.
2. a kind of thick lip skin-carp meat induced spawning method as claimed in claim 1, it is characterized in that: the little Chi of described numbering A ~ I, having during external water source and naturally enter row by respective discharge outlet of entering, manufacturing Inner eycle flowing water without during external water source by drawing water between little Chi.
3. a kind of thick lip skin-carp meat induced spawning method as claimed in claim 1, is characterized in that: the Rhizoma Gastrodiae powder contained in described tame and docile food hand fit high protein feed, and June, content monthly increased progressively by 0.01% to 0.1% to October; November, content monthly successively decreased by 0.1% to 0.01% to January.
4. a kind of thick lip skin-carp meat induced spawning method as claimed in claim 1, is characterized in that: described hasten parturition before reinforcing stimulus, can according to the different situations of gonad development in D, E, F pond, control flow check water speed, water temperature, light application time and intensity respectively; If parent population quantity very little, to reduce gonad development gap as far as possible, make its sexual cycle close to synchronous by different stimulated, avoid later stage artificial insemination parent population lazy weight; If parent population quantity is abundant, can pull open gonad development gap a little, make its sexual cycle inconsistent by different stimulated, shortage of manpower when avoiding later stage artificial insemination, the situation having little time to inseminate produces.
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Cited By (5)
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| CN104839069A (en) * | 2015-06-09 | 2015-08-19 | 广东海洋大学 | Method for acquiring batched Sillago sihama parent fishes |
| CN105941221A (en) * | 2016-05-16 | 2016-09-21 | 塔里木大学 | Reinforced cultivation method of parent fish of schizothorax biddulphi |
| CN106489798A (en) * | 2016-10-27 | 2017-03-15 | 武汉中科瑞华生态科技股份有限公司 | A kind of middle Warsaw loach artificial fecundation method |
| CN107950443A (en) * | 2017-12-29 | 2018-04-24 | 西南大学 | A kind of method for improving perch carp postpartum parent population survival rate |
| CN114375879A (en) * | 2021-12-29 | 2022-04-22 | 广东省鳇鲸海洋生物科技有限公司 | Breeding method for promoting gonad development of parent fish of yellow-lipped |
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| KR20130057722A (en) * | 2011-11-24 | 2013-06-03 | 전라남도 | Method for mass producing artificial seed of abudefduf vaigiensis |
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