CN105248336A - Artificial breeding method for glyptosternon maculatum - Google Patents
Artificial breeding method for glyptosternon maculatum Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides an artificial breeding method for glyptosternon maculatum. The artificial breeding method includes the following steps that A, after wild glyptosternon maculatum parent fishes are immersed in a saline solution, sex discrimination is conducted, and female fishes and male fishes are collected; B, a medicine composition for female fish spawning induction is prepared from 5 micrograms of lutropin releasing hormones and one carp hypophysis per kg of the female fishes, 24 hours after injection, the abdomens of the female fishes are slightly pressed, and if no free egg granule flows out, a second shot of spawning induction medicine of the same dosage is injected; C, dry method fertilization is adopted; D, after egg membranes of zygotes disengage, the zygotes are poured into an incubation tank to be incubated, when glyptosternon maculatum fry begin to swim at the bottom of a pool, exogenous nutrition is turned to, and animal live bait and artificial compound feed are fed. The nature rule of the wild glyptosternon maculatum parent fishes is grasped, the artificial propagation technology of glyptosternon maculatum is studied, a set of feasible artificial spawning induction and artificial incubation method is formed, and artificial large-scale breeding of glyptosternon maculatum is achieved.
Description
Technical field
The present invention relates to culture fishery, be specifically related to
the artificial breeding method of a kind of blackspot Yuan Select-Committee.
Background technology
Blackspot Yuan Select-Committee belongs to SILURIFORMES
siluriformes, Select-Committee section
sisoridae, Yuan Select-Committee belongs to
glyptosternum.For plateau cold water fishes, be distributed in Bradley Ma Putela river (Brahmaputra) and China of India
tibetthe areas such as Linzhi, Tang Jia, Rikaze, distribution height above sea level is from about 4200m to about 2800m, and area current are comparatively rapid, and water temperature is throughout the year lower than 15 DEG C.Blackspot Yuan Select-Committee Zuo Wei Mudfish Select-Committee fish (
glyptosternoidfish) one, taxonomy, biogeography and evolution situation are more common in its research in early days; Research in recent years focuses mostly on Yu Mudfish Select-Committee fish systematic growth and evolution aspect.Blackspot Yuan Select-Committee is the ominivore-fish partially moving feeding habits, and the food species of ingesting reaches more than 40 and plants, and fish are its main bait, are secondly algae, are oligochaetes and organic debris again.Blackspot Yuan Select-Committee is mainly distributed in the middle lower floor that river opens pool, especially under liking the stone of perch near many petrographic provinces and between gap, mainly dwell aufwuch for food the end of with, the mode of looking for food is travelling search and be attached to shovel on stone face and scrape in megalith peak, be not good at quick, long-distance swimming, chase the indifferent of fish.
Have scholar to think, the evolutionary development of blackspot Yuan Select-Committee is relevant with the grand several times liter of Qinghai-Tibet Platean.Though the output of Select-Committee section fish is little, because this kind of meat quality of fish is delicate, between flesh, thorn is few, deeply likes there is certain Fishery value by the masses.The Lhasa market price reaches 1000-1200 unit/kg, be carp, about 60 times of minister in ancient times fish price (14-16 unit/kg), economic benefit is higher.In June, 2008, blackspot Yuan Select-Committee artificial propagation obtains successfully, but also do not make substantial progress on domestication's breeding scale, before intensive culture is carried out to blackspot Yuan Select-Committee, domestication must be carried out and turn food, make the biological feed of the original facility of blackspot Yuan Select-Committee turn to artifical compound feed of ingesting, be just convenient to artificial large-scale farming.
200910061532.5, the patent of invention that name is called " induced spawning method of the former Select-Committee of blackspot ", adopts exogenous oxytocic drug, utilizes induced spawning method to impel the blackspot of physical maturity former Select-Committee parent population sexual gland to grow further, lay eggs, thus obtains fertilized egg.Step comprises (1) selects mature brood fish, by male and female individuality than being 2-3: 1 combo; (2) oxytocic drug is prepared: the pharmaceutical composition dosage that female parent population is hastened parturition is: 2mg maleic acid DOM/kg body weight+10-15mg luteotropin releasing hormone d-ala analog/kg body weight+3-5mg carp hypophysis/kg body weight.Male parent population oxytocic drug composition dosage is 1/2 of above-mentioned female parent population dosage; (3) by formula ratio, oxytocic drug is made pulpous state: (4) propose the medication of two pin injections.Because blackspot Yuan Select-Committee milter spermary is branch-like, seminal fluid is difficult to extrude, and this patented method adopts the method for extruding milter belly, obtains lack of quantity of spermatic fluid, large to milter demand.
201310699056.6, the application for a patent for invention that name is called " a kind of method adopting artificial feed to cultivate the former Select-Committee fry of blackspot and fingerling ", at indoor water mud sump, utilize healthy stream water source, Artificial Control miniflow water, feeding artifical compound feed, is the fingerling of total length 3cm by the fry rearing of hatching from fertilized egg.The fry adopting the present invention to cultivate blackspot Yuan Select-Committee 2 ~ 3 age in days is to the survival rate of 3cm fingerling to more than 80%.This patented method adopts cement pit artificial culture, does not meet modernization and propagates demand to energy-saving and emission-reduction artificially.
Summary of the invention
The present invention, in order to solve the problems of the technologies described above, provides
the artificial breeding method of a kind of blackspot Yuan Select-Committee.By grasping the Maturity Law of wild blackspot Yuan Select-Committee parent population, blackspot Yuan Select-Committee artificial propagation techniques being studied, forms a set of feasible artificial induced spawning, artificial incubation method, achieving the artificial large-scale farming of blackspot Yuan Select-Committee.
For achieving the above object, the present invention adopts following technical scheme:
The artificial breeding method of blackspot Yuan Select-Committee, is characterized in that: comprise the following steps:
The selection of A, parent population
Wild blackspot Yuan Select-Committee parent population after saline solution soaks, then carries out Sex judging, collects raun and milter;
Milter body surface is coarse, the little and depression of gonopore, raun body surface smooth, and gonopore is large and protrude, and color is general red.This feature is mainly used to differentiate female milter in reproduction period.
Preferably, employing mass fraction is the saline solution immersion 20-40min of 1%, and object kills the parasite by bringing in wild environment.
Preferably, the body weight of collecting raun is 0.065-0.25kg, and the body weight of milter is 0.3-0.48kg, and individuality is larger, and testis development is better.
B, artificial induced spawning
The dosage of the pharmaceutical composition of hastening parturition for raun is: luteinizing hormone releasing hormone 5 μ g/kg body weight+1 carp pituitary/kg body weight; Checking ecbolic effect, flicking raun belly after injection 24h, as flowed out without free ovum grain, then injecting same dosage oxytocic drug second pin;
Injection site be fish pectoral fin base portion without squama recess, injection angles is that 45° angle thrusts with syringe needle towards fish body front and body axle.
Because parent population used is wild parent population, raun gonad development degree is better, and belly is full, and therefore dosage used is less.
C, artificial insemination
Employing dry method is fertilized: is taken out by the spermary of milter, after grinding with normal saline flushing in the container filling fish-egg, smart ovum puts into incubation plate after mixing, and hatches in incubation tank;
Employing dry method is fertilized, and is just to activate because the sperm of fish meets water, the chances are the 40-50 of the Sperm survival time after activation second, therefore can not meet water, in order to avoid affect fertilization rate before smart ovum mixing.On the other hand, because blackspot Yuan Select-Committee milter spermary is branch-like, seminal fluid is difficult to extrude, and spermary can only be taken out, if directly extruded by seminal fluid, extrude sperm volume little, fertilization rate is not high, and milter belly injuredly also to be die because extruding.
Preferably, the ratio of described milter and raun is 1:4-6.The proportion of 1:4-6 determines according to milter spermary size, and fertilization rate is high, little to the demand of milter.
Preferably, to parent population intrathecal penicillin in postpartum, injected dose is 50000 international units/kg body weight.To parent population anti-inflammatory in postpartum, control is infected dead.
Preferably, the temperature in described incubation plate is 13-14 DEG C.Object in incubation plate is: in hatching process, egg membrane is directly fallen down by the mesh of tray bottom after coming off, and does not affect water quality.Start twisting Deng fry after rupture of membranes, tail can stick in the mesh of incubation plate stuck, therefore puts into fiberglass after rupture of membranes again and hatches.
D, seed rearing
After the egg membrane of fertilized egg comes off in incubation plate, fertilized egg is poured in incubation tank and hatches, when blackspot Yuan Select-Committee fry starts to move about at the bottom of pond, turn to exogenous nutrition, animality of throwing something and feeding live bait and artifical compound feed.
Described animality live bait is conventional animal live bait in the aquacultures such as cladocera, red line worm, wheel animalcule.
Preferably, described incubation tank is circulating water chennel, adopt cycle water, there is water saving, save land, efficiently, the advantage such as environmental protection.
Preferably, the water source in described incubation tank is underground water, and after the aeration of outdoor aeration basin, pump enters incubation tank, increases oxygen content in water.Underground water temperature is constant, pollution-free.
Preferably, the hatching water temperature in described incubation tank is 13-14 DEG C, and with inflator pump 24h blowing aeration, dissolved oxygen is at 6.0-7.2mg/L, and every day, rate of water exchange was 20%.Meet the hatching of fish-egg.
Preferably, described step C, fish-egg is put into incubation plate and is hatched, and in incubation tank, the depth of water is 0.25-0.35m, and described D step, the depth of water in incubation tank is adjusted to 0.4-0.5m.Increase water body amount in fishpond, fry hatching out after short-term endosome weight gain all can obviously increase, fry enters flat trip after date and can move about simultaneously, all needs larger activity space.Increase water body amount in fishpond simultaneously, be also conducive to water quality (the such as dissolved oxygen concentration etc.) big ups and downs slowed down because various unexpected factor causes.
Incubation tank of the present invention comprises tank and incubation plate, in described tank, dismountable incubation plate is installed, described incubation plate comprises framework and water fender, and described water fender is arranged at the below of a side elevation of framework, and water fender contacts for seamless with bottom incubation tank; The bottom surface of this side elevation and framework is network structure.
Cold water
fish fishegg hatching requires that fish-egg periphery will have good water coke slurry, and can not strenuous exercise at most of stage fish-egg, so, fish-egg to be placed on bottom incubation plate in net, water, from bottom toward upper reaches, is discharged from incubation plate side, is then entered bottom next incubation plate again, the like, until last incubation plate.
Preferably, the network structure aperture of described side elevation and framework bottom surface is 30 orders.Mesh is excessive, and fish-egg falls down, and mesh too small meeting anoxic, is determined by the fish-egg particle size of hatching and the fry size just hatched.
Preferably, have multiple incubation plate along water inlet side by side to delivery port direction in described incubation tank, water fender is arranged on delivery port direction.Current enter by bottom incubation plate, flow out, until discharge from the side elevation of last incubation plate from side elevation.
Hatching water temperature controls at 13 DEG C-14 DEG C, and needed for embryonic development, accumulated temperature scope is 2592 DEG C DEG C, and incubation rate reaches more than 70%.After membrane, most of prelarva is at incubation tank congregate, and yolk sac becomes ellipse from circle.Opening 60 days long 2.3cm of blackspot former Select-Committee fry average body, average weight 0.6g.
Beneficial effect of the present invention is:
1, because blackspot Yuan Select-Committee milter spermary is branch-like, seminal fluid is difficult to extrude, and spermary can only be taken out, if directly extruded by seminal fluid, extrude sperm volume little, fertilization rate is not high, and milter belly injuredly also to be die because extruding.The present invention adopts dry method to be fertilized, and directly takes out the spermary of milter, and obtain sperm volume large, little to the demand of milter, fertilization rate is high, and the ratio of milter and raun is only 1:4-6.
If the egg membrane 2, in hatching process is removed not in time, egg membrane is wrapped in the fry of firm rupture of membranes, easily causes anoxic, and easy long water mold.Seed rearing of the present invention adopts fertilized egg first to hatch in incubation plate, is directly fallen down by the mesh of tray bottom, need not manually be removed by egg membrane after egg membrane comes off.Fertilized egg is proceeded in incubation tank and hatches, can not because of the twisting of fry, stick in the mesh of incubation plate and die, incubation rate reaches more than 70%.
3, the present invention adopts recirculated water hatching, temperature-controllable, be convenient to management, industrial circulating water cultivating system is with industrialization means ACTIVE CONTROL water environment, water resources consumption is little, take up an area less, environmental pollution is little, product high-quality and safety, disease are few, density is high, breeding production by region or weather restriction and affect, resource utilization is high, and be high investment high production, low-risk realizes the important channel of culture fishery sustainable development.
4, incubation plate is placed in incubation tank, can ensure that fish-egg fry all cultivates in same set of system, concrete operating process also can ensure that fish-egg, fry do not have the chance of any disengaging water body, can avoid because fall damage that pond brings to fish-egg, fry and stress as far as possible.
5, the hatch fish roe of cold water fish requires that fish-egg periphery will have good water coke slurry, and can not strenuous exercise at most of stage fish-egg.This
inventincubation plate arranges water fender, water is not passed from the neutral gear between incubation plate and incubation tank, and must pass through from net bottom incubation plate, fish-egg to be placed on bottom incubation plate in net, water is from bottom toward upper reaches, flow through fish-egg, then go out from the logistics of incubation plate side mesh, ensure current trend.
Accompanying drawing explanation
fig. 1for the structural representation of hatch fish roe groove of the present invention
figure.
fig. 2for the structural representation of incubation plate of the present invention
figure.
in figurebe labeled as: 1, incubation tank, 2, incubation plate, 3, aeration oxygen replenishing device, 4, side elevation, 5, framework, 6, water fender.
Embodiment
Below in conjunction with embodiment, essentiality content of the present invention is described in further detail.
Embodiment 1
the artificial breeding method of a kind of blackspot Yuan Select-Committee, comprise the following steps:
The selection of A, parent population
Wild blackspot Yuan Select-Committee parent population after saline solution soaks, then carries out Sex judging, collects raun and milter;
B, artificial induced spawning
The dosage of the pharmaceutical composition of hastening parturition for raun is: luteinizing hormone releasing hormone 5 μ g/kg body weight+1 carp pituitary/kg body weight; After injection 24h, flicking raun belly, as flowed out without free ovum grain, then injects same dosage oxytocic drug second pin;
C, artificial insemination
Employing dry method is fertilized: is taken out by the spermary of milter, after grinding with normal saline flushing in the container filling fish-egg, smart ovum puts into incubation plate after mixing, and hatches in incubation tank;
D, seed rearing
After the egg membrane of fertilized egg comes off, fertilized egg is poured in incubation tank and hatches, when blackspot Yuan Select-Committee fry starts to move about at the bottom of pond, turn to exogenous nutrition, animality of throwing something and feeding live bait and artifical compound feed.
Embodiment 2
The present embodiment is on the basis of embodiment 1:
Described step A, employing mass fraction is the saline solution immersion 20min of 1%.
Embodiment 3
The present embodiment is on the basis of embodiment 1:
Described step A, employing mass fraction is the saline solution immersion 40min of 1%.
Described step A, the body weight of collecting raun is 0.065kg, and the body weight of milter is 0.38kg.
Embodiment 4
The present embodiment is on the basis of embodiment 1:
Described step A, employing mass fraction is the saline solution immersion 25min of 1%.
Described step A, the body weight of collecting raun is 0.25kg, and the body weight of milter is 0.48kg.
The milter of described step C and the ratio of raun are 1:4.
Embodiment 5
The present embodiment is on the basis of embodiment 1:
Described step A, employing mass fraction is the saline solution immersion 30min of 1%.
Described step A, the body weight of collecting raun is 0.15kg, and the body weight of milter is 0.35kg.
The milter of described step C and the ratio of raun are 1:6.
The incubation plate of described step C is 30 object net dishes.
Embodiment 6
The present embodiment is on the basis of embodiment 1:
Described step A, employing mass fraction is the saline solution immersion 35min of 1%.
Described step A, the body weight of collecting raun is 0.08kg, and the body weight of milter is 0.4kg.
The milter of described step C and the ratio of raun are 1:5.
The incubation plate of described step C is 30 object net dishes.
Described incubation tank is circulating water chennel.
Embodiment 7
The present embodiment is on the basis of embodiment 1:
Described step A, employing mass fraction is the saline solution immersion 28min of 1%.
Described step A, the body weight of collecting raun is 0.1kg, and the body weight of milter is 0.45kg.
The milter of described step C and the ratio of raun are 1:4.5.
The incubation plate of described step C is 30 object net dishes.
Described incubation tank is circulating water chennel.
Water source in described incubation tank is underground water, and after the aeration of outdoor aeration basin, pump enters incubation tank.
Embodiment 8
The present embodiment is on the basis of embodiment 1:
Described step A, employing mass fraction is the saline solution immersion 25min of 1%.
Described step A, the body weight of collecting raun is 0.085kg, and the body weight of milter is 0.32kg.
The milter of described step C and the ratio of raun are 1:5.5.
The incubation plate of described step C is 30 object net dishes.
Described incubation tank is circulating water chennel.
Water source in described incubation tank is underground water, and after the aeration of outdoor aeration basin, pump enters incubation tank.
Hatching water temperature in described incubation tank is 13 DEG C, and with inflator pump 24h blowing aeration, dissolved oxygen is at 6.0mg/L, and every day, rate of water exchange was 20%.
Embodiment 9
The present embodiment is on the basis of embodiment 1:
Described step A, employing mass fraction is the saline solution immersion 36min of 1%.
Described step A, the body weight of collecting raun is 0.2kg, and the body weight of milter is 0.42kg.
The milter of described step C and the ratio of raun are 1:4.
The incubation plate of described step C is 30 object net dishes.
Described incubation tank is circulating water chennel.
Water source in described incubation tank is underground water, and after the aeration of outdoor aeration basin, pump enters incubation tank.
Hatching water temperature in described incubation tank is 14 DEG C, and with inflator pump 24h blowing aeration, dissolved oxygen is at 7.2mg/L, and every day, rate of water exchange was 20%.
To parent population intrathecal penicillin in postpartum, injected dose is 50000 international units/kg body weight.
Embodiment 10
The present embodiment is on the basis of embodiment 1:
Described step A, employing mass fraction is the saline solution immersion 30min of 1%.
Described step A, the body weight of collecting raun is 0.09kg, and the body weight of milter is 0.35kg.
The milter of described step C and the ratio of raun are 1:5.
The incubation plate of described step C is 30 object net dishes.
Described incubation tank is circulating water chennel.
Water source in described incubation tank is underground water, and after the aeration of outdoor aeration basin, pump enters incubation tank.
Hatching water temperature in described incubation tank is 13.5 DEG C, and with inflator pump 24h blowing aeration, dissolved oxygen is at 6.5mg/L, and every day, rate of water exchange was 20%.
To parent population intrathecal penicillin in postpartum, injected dose is 50000 international units/kg body weight.
Described step C, fish-egg is put into incubation plate and is hatched, and in incubation tank, the depth of water is 0.25m, and described D step, the depth of water in incubation tank is adjusted to 0.4m.
Embodiment 11
The present embodiment is on the basis of embodiment 1:
Described step A, employing mass fraction is the saline solution immersion 30min of 1%.
Described step A, the body weight of collecting raun is 0.09kg, and the body weight of milter is 0.35kg.
The milter of described step C and the ratio of raun are 1:5.
The incubation plate of described step C is 30 object net dishes.
Described incubation tank is circulating water chennel.
Water source in described incubation tank is underground water, and after the aeration of outdoor aeration basin, pump enters incubation tank.
Hatching water temperature in described incubation tank is 13.5 DEG C, and with inflator pump 24h blowing aeration, dissolved oxygen is at 6.5mg/L, and every day, rate of water exchange was 20%.
To parent population intrathecal penicillin in postpartum, injected dose is 50000 international units/kg body weight.
Described step C, fish-egg is put into incubation plate and is hatched, and in incubation tank, the depth of water is 0.35m, and described D step, the depth of water in incubation tank is adjusted to 0.5m.
Embodiment 12
The present embodiment is on the basis of embodiment 1:
Described step A, employing mass fraction is the saline solution immersion 30min of 1%.
Described step A, the body weight of collecting raun is 0.09kg, and the body weight of milter is 0.35kg.
The milter of described step C and the ratio of raun are 1:5.
The incubation plate of described step C is 30 object net dishes.
Described incubation tank is circulating water chennel.
Water source in described incubation tank is underground water, and after the aeration of outdoor aeration basin, pump enters incubation tank.
Hatching water temperature in described incubation tank is 13.5 DEG C, and with inflator pump 24h blowing aeration, dissolved oxygen is at 6.5mg/L, and every day, rate of water exchange was 20%.
To parent population intrathecal penicillin in postpartum, injected dose is 50000 international units/kg body weight.
Described step C, fish-egg is put into incubation plate and is hatched, and in incubation tank, the depth of water is 0.3m, and described D step, the depth of water in incubation tank is adjusted to 0.45m.
Embodiment 13
The present embodiment is on the basis of embodiment 1:
Described incubation tank comprises tank and incubation plate, in described tank, dismountable incubation plate is installed, described incubation plate comprises framework and water fender, and described water fender is arranged at the below of a side elevation of framework, and water fender contacts for seamless with bottom incubation tank; The bottom surface of this side elevation and framework is network structure.
Embodiment 14
The present embodiment is on the basis of embodiment 9:
Described incubation tank comprises tank and incubation plate, in described tank, dismountable incubation plate is installed, described incubation plate comprises framework and water fender, and described water fender is arranged at the below of a side elevation of framework, and water fender contacts for seamless with bottom incubation tank; The bottom surface of this side elevation and framework is network structure.
The network structure aperture of described side elevation and framework bottom surface is 30 orders.
Embodiment 15
The present embodiment is on the basis of embodiment 10:
Described incubation tank comprises tank and incubation plate, in described tank, dismountable incubation plate is installed, described incubation plate comprises framework and water fender, and described water fender is arranged at the below of a side elevation of framework, and water fender contacts for seamless with bottom incubation tank; The bottom surface of this side elevation and framework is network structure.
The network structure aperture of described side elevation and framework bottom surface is 30 orders.
Have multiple incubation plate along water inlet side by side to delivery port direction in described incubation tank, water fender is arranged on delivery port direction.
Claims (11)
1. an artificial breeding method of the former Select-Committee of blackspot, is characterized in that: comprise the following steps:
The selection of A, parent population
Wild blackspot Yuan Select-Committee parent population after saline solution soaks, then carries out Sex judging, collects raun and milter;
B, artificial induced spawning
The dosage of the pharmaceutical composition of hastening parturition for raun is: luteinizing hormone releasing hormone 5 μ g/kg body weight+1 carp pituitary/kg body weight; After injection 24h, flicking raun belly, as flowed out without free ovum grain, then injects same dosage oxytocic drug second pin;
C, artificial insemination
Employing dry method is fertilized: is taken out by the spermary of milter, after grinding with normal saline flushing in the container filling fish-egg, smart ovum puts into incubation plate after mixing, and hatches in incubation tank;
D, seed rearing
After the egg membrane of fertilized egg comes off, fertilized egg is poured in incubation tank and hatches, when blackspot Yuan Select-Committee fry starts to move about at the bottom of pond, turn to exogenous nutrition, animality of throwing something and feeding live bait and artifical compound feed.
2. the artificial breeding method of a kind of blackspot Yuan Select-Committee according to claim 1, is characterized in that: described step A, and employing mass fraction is the saline solution immersion 20-40min of 1%.
3. the artificial breeding method of a kind of blackspot Yuan Select-Committee according to claim 1, is characterized in that: described step A, and the body weight of collecting raun is 0.065-0.25kg, and the body weight of milter is 0.3-0.48kg.
4. the artificial breeding method of a kind of blackspot Yuan Select-Committee according to claim 1, is characterized in that: the milter of described step C and the ratio of raun are 1:4-6.
5. the artificial breeding method of a kind of blackspot Yuan Select-Committee according to claim 1, it is characterized in that: described incubation tank comprises tank and incubation plate, in described tank, dismountable incubation plate is installed, described incubation plate comprises framework and water fender, described water fender is arranged at the below of a side elevation of framework, and water fender contacts for seamless with bottom incubation tank; The bottom surface of this side elevation and framework is network structure.
6. the artificial breeding method of a kind of blackspot Yuan Select-Committee according to claim 5, is characterized in that: the network structure aperture of described side elevation and framework bottom surface is 30 orders.
7. the artificial breeding method of a kind of blackspot Yuan Select-Committee according to claim 5, is characterized in that: have multiple incubation plate along water inlet side by side to delivery port direction in described incubation tank, water fender is arranged on delivery port direction.
8. the artificial breeding method of a kind of blackspot Yuan Select-Committee according to claim 1, it is characterized in that: described incubation tank is circulating water chennel, water source is underground water, and after the aeration of outdoor aeration basin, pump enters incubation tank.
9. the artificial breeding method of a kind of blackspot Yuan Select-Committee according to claim 1, is characterized in that: the hatching water temperature in described incubation tank is 13-14 DEG C, and with inflator pump 24h blowing aeration, dissolved oxygen is at 6.0-7.2mg/L, and every day, rate of water exchange was 20%.
10. the artificial breeding method of a kind of blackspot Yuan Select-Committee according to claim 1, it is characterized in that: to parent population intrathecal penicillin in postpartum, injected dose is 50000 international units/kg body weight.
The artificial breeding method of 11. a kind of blackspot Yuan Select-Committee according to claim 1, it is characterized in that: described step C, fish-egg is put into incubation plate and is hatched, and in incubation tank, the depth of water is 0.25-0.35m, described D step, the depth of water in incubation tank is adjusted to 0.4-0.5m.
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| CN109845687A (en) * | 2019-04-18 | 2019-06-07 | 中国水产科学研究院黑龙江水产研究所 | Blackspot Yuan Select-Committee cultivates the circulating water culture system of domestication |
| CN109937953A (en) * | 2019-04-18 | 2019-06-28 | 中国水产科学研究院黑龙江水产研究所 | The method that blackspot Yuan Select-Committee cultivates domesticating and cultivating is carried out using recirculated water |
| CN111449006A (en) * | 2020-04-23 | 2020-07-28 | 中国水产科学研究院黑龙江水产研究所 | Artificial domestication method of wild parent fishes of Glyptosternum maculatum |
| CN111567450A (en) * | 2020-05-12 | 2020-08-25 | 中国水产科学研究院黑龙江水产研究所 | Artificial induced spawning and low-temperature seed culture method of Glyptosternum maculatum |
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| CN107581110A (en) * | 2017-11-01 | 2018-01-16 | 西藏自治区农牧科学院水产科学研究所 | The Supply Method of blackspot Yuan Select-Committee open-mouthed baits |
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| CN109937953A (en) * | 2019-04-18 | 2019-06-28 | 中国水产科学研究院黑龙江水产研究所 | The method that blackspot Yuan Select-Committee cultivates domesticating and cultivating is carried out using recirculated water |
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| CN111449006A (en) * | 2020-04-23 | 2020-07-28 | 中国水产科学研究院黑龙江水产研究所 | Artificial domestication method of wild parent fishes of Glyptosternum maculatum |
| CN111567450A (en) * | 2020-05-12 | 2020-08-25 | 中国水产科学研究院黑龙江水产研究所 | Artificial induced spawning and low-temperature seed culture method of Glyptosternum maculatum |
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