CN104221952B - A kind of Chalcalbumus chalcoides aralensis artificial fecundation method - Google Patents
A kind of Chalcalbumus chalcoides aralensis artificial fecundation method Download PDFInfo
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- CN104221952B CN104221952B CN201310728350.5A CN201310728350A CN104221952B CN 104221952 B CN104221952 B CN 104221952B CN 201310728350 A CN201310728350 A CN 201310728350A CN 104221952 B CN104221952 B CN 104221952B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a kind of Chalcalbumus chalcoides aralensis artificial fecundation method, comprise the following steps: parent fish rearing and selecting, artificial induced spawning, Process of Insemination, hatching, the invention provides a kind of method reasonable, workable, be produced on a large scale the whole artificial propagation method of Chalcalbumus chalcoides aralensis seed, by optimizing parent fish rearing technology, three kinds of different oxytocic drugs are used to carry out artificial induced spawning, artificial insemination, improve the spawning rate of breeding parent fish, fish roe rate of fertilization and fry hatching rate, parent fish spawning rate brings up to 88.2 %, fish roe rate of fertilization brings up to 97.0%, fry hatching rate is up to 83.64%, this method is scientific and reasonable, workable, utilize this technology can realize factorial praluction, Seedling kind is provided for large-scale farming, obtain good economic benefit.
Description
Technical field
The present invention relates to artificial freshwater-fish breeding method, particularly to a kind of Chalcalbumus chalcoides aralensis artificial fecundation method.
Background technology
It is white that Chalcalbumus chalcoides aralensis (Chalcalburnus Chalcoides aralensis) is subordinate to Cyprinidae, refined sieve subfamily, OK a karaoke club
Fish belongs to.This fishing gear has the merits such as strong stress resistance, feeding habits are wide, meat is good, is the famous and precious Species of Rare Fish from Qingdao of Central Asia.Chalcalbumus chalcoides aralensis
Originally being wild species, calendar year 2001 is introduced China first by one of applicant.
At present, the research breeding biology and aquaculture organism aspect of Chalcalbumus chalcoides aralensis has been reported.The introduction of wild fish,
Domestication and breeding and pond cultivation technology successfully, and achieve effect at China's saline-alkali water and freshwater cultivation.But,
Also be formed without the technical system of a set of maturation at artificial propagation, parent fish rearing, induced spawning method, incubating oosperm aspect still suffer from
Problems.Owing to this fish individuality is little, fish brood amount is low, and parent fish individuality gonad development differs greatly, and how ovary development accelerating is same
Step, it is ensured that parent fish quantity of laying eggs and concentration egg laying amount, improves overall rate of fertilization, incubation rate and the survival rate of whole artificial propagation fish,
It it is the key point realizing Chalcalbumus chalcoides aralensis seed large-scale production.
Summary of the invention
The present invention is directed to the problems referred to above and researched and developed a kind of Chalcalbumus chalcoides aralensis artificial fecundation method, its object is to: provide
A kind of method is reasonable, workable, the whole artificial propagation method of the Chalcalbumus chalcoides aralensis seed that is produced on a large scale, by optimizing parent fish
Cultivating techniques, uses three kinds of different oxytocic drugs to carry out artificial induced spawning, artificial insemination, improves the breeding spawning rate of parent fish, fish roe
Rate of fertilization and fry hatching rate.
Technical solution of the present invention:
A kind of Chalcalbumus chalcoides aralensis artificial fecundation method, it is characterised in that: described a kind of Chalcalbumus chalcoides aralensis artificial fecundation method is one
Kind of Chalcalbumus chalcoides aralensis artificial induced spawning is inseminated propagation method naturally, comprises the following steps:
1, parent fish rearing and selecting: produce and incubate first 30-40 days, select 3 age in winter parent fish, the milter body weight selected is 45-60g,
Raun body weight is 60-100g, and divides pond reinforced cultivating by female milter, and feedstuff is bean cake, day feeding volume be the 5-8% of body weight；
2, artificial induced spawning: when constant about 24 DEG C of water temperature, select parent fish and hasten parturition from storage pond, holding pond, injection promotees corpus luteum
Releasing hormone analog (LRH-A2), maleic acid DOM (DOM) and chorionic-gonadotropin hormone (HCG), three's hybrid injection,
Leuteinizing hormone releasing hormone analogue (LRH-A2), maleic acid DOM (DOM) and chorionic-gonadotropin hormone (HCG) injection dosage
For: LRH-A2, 3-5 g/kg, DOM, 2mg/ kg, HCG, 400-600UI/ kg, use pectoral fin lumbar injection, milter injection
Amount halves；
3, Process of Insemination: using artificial induced spawning naturally to inseminate, the parent fish after hastening parturition puts into case of laying eggs, and case of laying eggs uses
100 mesh sieve silk sewings form, and specification is long 2.5m × wide 1.0m × high 1.0m, and case of laying eggs is placed in indoor water mud sump, cement
Water body is long 5m × wide 8m × high 1.3m, and 100 groups of fishes thrown in by every net cage, lays eggs and oxygen supplement with water pump circulation stimulation by running water fish,
Utilizing heater and recirculated water debugging water temperature to 23-25 DEG C, after hastening parturition during drug influence time 14-18h, inspection is laid eggs
Case, finds, when water body has a large amount of germ cell, to be removed by parent fish, collects germ cell；
4, cylinder hatching barrel hatching: germ cell, after clear water rinses, is poured cylinder hatching barrel into, put about 50,000 ovum, incubate for every barrel
Change bucket diameter 15cm, high 40cm, be placed in the incubation tank filling clear water, use the filter micro-Lotic hatching of backpulsing, incubation tank
Water is changed 2-3 time every day, when hatching barrel 50% fry membrane, is poured in mounted planar slot mesh sheet by fish embryo in bucket, hatching
Water temperature keeps 23-25 DEG C, fish embryo 58-72h membrane.
A kind of Chalcalbumus chalcoides aralensis artificial fecundation method, it is characterised in that: described a kind of Chalcalbumus chalcoides aralensis artificial fecundation method is one
Plant Chalcalbumus chalcoides aralensis artificial induced spawning intervention insemination propagation method, comprise the steps:
1, parent fish rearing and selecting: produce and incubate first 30-40 days, select 3 age in winter parent fish, the milter body weight selected is 45-60g,
Raun body weight is 60-100g, and divides pond reinforced cultivating by female milter, and feedstuff is bean cake, day feeding volume be the 5-8% of body weight；
2, artificial induced spawning: when constant about 24 DEG C of water temperature, select parent fish and hasten parturition from storage pond, holding pond, injection promotees Huang
Body releasing hormone analog (LRH-A2), maleic acid DOM (DOM) and chorionic-gonadotropin hormone (HCG), three mixes note
Penetrate, Leuteinizing hormone releasing hormone analogue (LRH-A2), maleic acid DOM (DOM) and chorionic-gonadotropin hormone (HCG) injection
Dosage is: LRH-A2, 3-5 g/ kg, DOM, 2mg/ kg, HCG, 400-600UI/ kg, use pectoral fin lumbar injection, milter note
Penetrate dosage to halve；
3, Process of Insemination: use manual intervention insemination, the female milter hastened parturition is divided and is placed in different egg case, follow with water pump
Circulating water stimulate fish lay eggs and oxygen supplement, utilize heater debugging water temperature to 23-25 DEG C, after parent fish is hastened parturition, when drug influence
Between during 14-18h, check raun, gently extruding raun abdominal part every 1h, gonopore has a small amount of ovum grain to flow out, carries out intervention and be subject to
Essence, every 3 female fish roes of tail and the insemination of 1 tail milter seminal fluid, pour taper hatching barrel into；
4, taper hatching barrel hatching: after germ cell being washed with clear water, stir 30min with feather, will after ovum mucus comes off
It puts into hatching barrel, taper hatching barrel volume 109.4L, about every barreled 500,000 ovum, uses micro-Lotic hatching, fish embryonic development
60h, when fry hatches 1/3 quantity, will hatch prelarva and constantly remove hatching barrel, pour circular groove into and carries out fry rearing, hatch water
Temperature keeps 23-25 DEG C, fish embryo 58-72h membrane.
The method have the benefit that
The invention provides that a kind of method is reasonable, workable, the Chalcalbumus chalcoides aralensis seed that is produced on a large scale complete artificial
Propagation method, by optimizing parent fish rearing technology, uses three kinds of different oxytocic drugs to carry out artificial induced spawning, artificial insemination, improves
The breeding spawning rate of parent fish, fish roe rate of fertilization and fry hatching rate, parent fish spawning rate brings up to 88.2 %, fish roe rate of fertilization carries
High to 97.0%, fry hatching rate is up to 83.64%, and this method is scientific and reasonable, workable, utilizes this technology can realize work
Factory's metaplasia is produced, and provides seed for large-scale farming, it is thus achieved that good economic benefit.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further.
Embodiment:
1, parent fish rearing with select
Product is incubated first 30 days, select 3 age in winter parent fish, and divide pond reinforced cultivating by female milter, feedstuff is bean cake, day feeding volume be
The 5-8% of body weight, selectivity maturation raun 100 tail, abdominal part is full, soft, and gonopore mastoid process is big and highlights, Kong Yuancheng blush, entirely
Long 19.22-23.71cm, body weight 55.96-97.46g, selectivity maturation milter 120 tail, total length 17.89-20.61cm, body weight
45.11-65.60g, health is slender, and healthy scale is complete, and at the head gill cover, hands is touched and coarse had star-pursuing, and extruding gonopore has gently
Seminal fluid flows out, and seminal fluid is met water and scattered rapidly.
2, artificial induced spawning
When constant 23-25 DEG C of water temperature, from storage pond, holding pond, select parent fish (female-male proportion is 1:1.2) hasten parturition, injection
Medicine be Ningbo bio-pharmaceuticals three factory produce Leuteinizing hormone releasing hormone analogue (LRH-A2), maleic acid DOM (DOM) and
Chorionic-gonadotropin hormone (HCG), three's hybrid injection, injection dosage is: LRH-A23-5µg/ kg、DOM 2mg/ kg、HCG
400-600UI/ kg.Pectoral fin lumbar injection, milter injection dosage is used to halve.
3, fertilization process
Natural spawning: parent fish of hastening parturition puts into case of laying eggs, lays eggs and oxygen supplement with water pump circulation stimulation by running water fish, utilizes heating
Device recirculated water debugging water temperature is to 23-25 DEG C, and drug influence time 14-18h, after the 18h that hastens parturition, by parent fish removal, collection fertilization
Ovum.
Artificial insemination: at drug influence time 14-18h, check raun gonopore every 1h and extrude abdominal part gently, finding
Having ovum grain to flow out, immediate intervention is fertilized, and germ cell is rinsed with clear water and feather stirs 30min, pours cone hatching barrel into and (puts for every barrel
500000 ovum), micro-Lotic hatching.
4, incubating oosperm process
Germ cell, after clear water rinses, pours cylinder hatching barrel into.Putting about 50,000 ovum for every barrel, hatching barrel is placed in fills clear water
Incubation tank in, use the micro-Lotic hatching of filter backpulsing, incubation tank water is changed 2-3 time every day, when hatching barrel 50% fry goes out
During film, being poured in mounted planar slot mesh sheet by fish embryo in bucket, hatching water temperature keeps 23-25 DEG C, fish fetal development about 58-
72h membrane.
Table 1 is gained testing result of the present invention
Claims (2)
1. a Chalcalbumus chalcoides aralensis artificial fecundation method, it is characterised in that: described a kind of Chalcalbumus chalcoides aralensis artificial fecundation method is a kind of
Chalcalbumus chalcoides aralensis artificial induced spawning is inseminated propagation method naturally, comprises the following steps:
1), parent fish rearing and selecting: produce and incubate first 30-40 days, select 3 age in winter parent fish, the milter body weight selected is 45-60g, raun
Body weight is 60-100g, and divides pond reinforced cultivating by female milter, and feedstuff is bean cake, day feeding volume be the 5-8% of body weight；
2), artificial induced spawning: when constant about 24 DEG C of water temperature, selecting parent fish and hasten parturition from storage pond, holding pond, injection promotees corpus luteum and releases
Put hormone analogs (LRH-A2), maleic acid DOM (DOM) and chorionic-gonadotropin hormone (HCG), three's hybrid injection, promote
Luteinizing hormone releasing hormone analog (LRH-A2), maleic acid DOM (DOM) and chorionic-gonadotropin hormone (HCG) injection dosage
For: LRH-A2, 3-5 g/kg, DOM, 2mg/ kg, HCG, 400-600UI/ kg, use pectoral fin lumbar injection, milter injection
Amount halves；
3), Process of Insemination: using artificial induced spawning naturally to inseminate, the parent fish after hastening parturition puts into case of laying eggs, and case of laying eggs uses 100 mesh
The sewing of bolting silk cloth forms, and specification is long 2.5m × wide 1.0m × high 1.0m, and case of laying eggs is placed in indoor water mud sump, cement pit water body
For long 5m × wide 8m × high 1.3m, 100 groups of fishes thrown in by every net cage, lay eggs and oxygen supplement with water pump circulation stimulation by running water fish, and utilization adds
Thermal and recirculated water debugging water temperature, to 23-25 DEG C, after hastening parturition during drug influence time 14-18h, check case of laying eggs, send out
When existing water body has a large amount of germ cell, parent fish is removed, collect germ cell；
4), cylinder hatching barrel hatching: germ cell, after clear water rinses, is poured cylinder hatching barrel into, put about 50,000 ovum for every barrel, hatching
Bucket diameter 15cm, high 40cm, be placed in the incubation tank filling clear water, uses the filter micro-Lotic hatching of backpulsing, incubation tank water
Every day is changed 2-3 time, when hatching barrel 50% fry membrane, is poured in mounted planar slot mesh sheet by fish embryo in bucket, hatches water
Temperature keeps 23-25 DEG C, fish embryo 58-72h membrane.
2. a Chalcalbumus chalcoides aralensis artificial fecundation method, it is characterised in that: described a kind of Chalcalbumus chalcoides aralensis artificial fecundation method is a kind of
Chalcalbumus chalcoides aralensis artificial induced spawning intervention insemination propagation method, comprises the steps:
1), parent fish rearing and selecting: produce and incubate first 30-40 days, select 3 age in winter parent fish, the milter body weight selected is 45-60g, raun
Body weight is 60-100g, and divides pond reinforced cultivating by female milter, and feedstuff is bean cake, day feeding volume be the 5-8% of body weight；
2), artificial induced spawning: when constant about 24 DEG C of water temperature, selecting parent fish and hasten parturition from storage pond, holding pond, injection promotees corpus luteum and releases
Put hormone analogs (LRH-A2), maleic acid DOM (DOM) and chorionic-gonadotropin hormone (HCG), three's hybrid injection, promote
Luteinizing hormone releasing hormone analog (LRH-A2), maleic acid DOM (DOM) and chorionic-gonadotropin hormone (HCG) injection dosage
For: LRH-A2, 3-5 g/ kg, DOM, 2mg/ kg, HCG, 400-600UI/ kg, use pectoral fin lumbar injection, milter injection
Amount halves；
3), Process of Insemination: use manual intervention insemination, the female milter hastened parturition is divided and is placed in different egg case, circulate with water pump
Stimulation by running water fish lays eggs and oxygen supplement, utilize heater debugging water temperature to 23-25 DEG C, after parent fish is hastened parturition, in the drug influence time
During 14-18h, checking raun, gently extruding raun abdominal part every 1h, gonopore has a small amount of ovum grain to flow out, and carries out intervening fertilization,
Every 3 female fish roes of tail and the insemination of 1 tail milter seminal fluid, pour taper hatching barrel into；
4), taper hatching barrel hatching: after germ cell being washed with clear water, stir 30min with feather, put after ovum mucus comes off
Enter hatching barrel, taper hatching barrel volume 109.4L, about every barreled 500,000 ovum, use micro-Lotic hatching, fish embryonic development 60h, when
When fry hatches 1/3 quantity, will hatch prelarva and constantly remove hatching barrel, and pour circular groove into and carry out fry rearing, hatching water temperature keeps
23-25 DEG C, fish embryo 58-72h membrane.
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| CN107333680A (en) * | 2016-04-29 | 2017-11-10 | 岚皋县绿水生态农业有限责任公司 | A kind of artificial breeding method of more squama shovel jaw fishes and special hatchery |
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| CN110896892A (en) * | 2019-12-17 | 2020-03-24 | 昆明市水产科学研究所 | Artificial breeding method for silver whitefish |
| CN113287548B (en) * | 2021-07-05 | 2022-11-25 | 镇康县水产技术推广站 | Artificial propagation method for yellow-shelled fishes |
| CN113545306A (en) * | 2021-07-14 | 2021-10-26 | 安徽冠禅生物科技有限公司 | Breeding method of freshwater fish |
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