CN103960188B - A kind of long Qi Wen Minnow induced spawning method - Google Patents
A kind of long Qi Wen Minnow induced spawning method Download PDFInfo
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- CN103960188B CN103960188B CN201410212376.9A CN201410212376A CN103960188B CN 103960188 B CN103960188 B CN 103960188B CN 201410212376 A CN201410212376 A CN 201410212376A CN 103960188 B CN103960188 B CN 103960188B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a kind of long Qi Wen Minnow induced spawning method.It is 1:1.5 that the inventive method is hastened parturition with mature brood fish female-male proportion; Oxytocic drug is luteotropin releasing hormone d-ala analog, and dosage is 6 μ g/kg fish body weight; Artificial insemination procedures is: fish for and wait to produce raun, dry fish body moisture, ovum is got in drying receptacle, fish for again and wait to produce milter, dry gonopore, draw seminal fluid with syringe, put into spermatozoa diluent to mix with ovum again, activate with clear water, leave standstill after 2 minutes, pour fertilized egg into incubator and hatch; In fertilized egg demoulding preincubation device, water volume flow rate is 0.4m/s, and after demoulding, flow velocity is 0.2m/s, can carry out seed rearing after demoulding after incubator is developed to the inflation of prelarva fish glue.Spawning rate of the present invention is greater than 65%, and fertilization rate is greater than 85%, and incubation rate is greater than 80%, can provide a large amount of prelarva, long Qi Wen Minnow enhancement releasing is achieved for producing long Qi Wen Minnow size seedling seed in batches.
Description
Technical field
The present invention relates to aquatic products artificial propagation techniques, be specifically related to a kind of long Qi Wen Minnow induced spawning method.
Background technology
Long Qi Wen Minnow belongs to Cypriniformes, Cyprinidae, Minnow subfamily, Wen Minnow belongs to, and popular name soil consumption youngster, foreign fish are Upper Yangtze River endemic species.Mainly be distributed in Jinsha jiang River, Upper Yangtze River master stream, Yalongjiang River, Ming River, Tuo Jiang, Jia Lingjiang River middle and lower reaches and Lower Reaches of Wujiang River.Its individuality is little, and growth is comparatively slow, and former the Changjiang river is fished in fishery and generally divided into " small-sized wild fishes ".In recent years, due to the change of Upper Yangtze River ecotope, its stock number is on a declining curve, and for protecting this kind of Upper Yangtze River Endemic fish resource, carrying out enhancement releasing is one of major measure of taking of current China.Producing long Qi Wen Minnow seed in batches is the precondition of carrying out its enhancement releasing, but China mainly concentrated on the aspect such as resource investigation and assessment, Age and growth, biology and genetic diversity to its research in the past, do not carry out its artificial propagation techniques research work, long Qi Wen Minnow artificial propagation techniques is the technical bottleneck of carrying out its enhancement releasing at present, and artificial induced spawning is the key of artificial propagation techniques.
Long Qi Wen Minnow artificial induced spawning technology is still in conceptual phase, the main method adopted is the mature brood fish utilizing field acquisition, carry out artificial induced spawning immediately, use oxytocic drug kind and entry needle time many, dosage is large, water quality index of hastening parturition is indefinite, spawning rate (below 5%) and fertilization rate low, technology poor repeatability.
Summary of the invention
The object of the invention is to overcome the shortcoming of prior art and deficiency, a kind of long Qi Wen Minnow induced spawning method is provided.The method to store up the long Qi Wen Minnow mature brood fish of more than 1 year under artificial condition by selecting, with appropriate oxytocic drug and induced spawning method, realize long Qi Wen Minnow artificial propagation, make batch production enhancement releasing size seedling seed become possibility.
Object of the present invention is achieved through the following technical solutions:
A kind of long Qi Wen Minnow induced spawning method, comprises the steps:
(1) parent population is selected: select to store up more than the 1 year anosis maturation long Qi Wen Minnow without wound, physique stalwartness as hastening parturition with parent population from closed recirculating cultivating system, parent population female-male proportion is arranged in pairs or groups by 1:1.5.
(2) artificial induced spawning: the parent population of selection is put into spawning inducing pool, parent population is placed density and is less than 10 tails/m
2; Pool inner water body keeps miniflow, and flow control is between 0.1-0.2m/s, and water temperature controls at 16-19 DEG C, and oxygen in water remains on more than 8mg/L, and pH is between 7.0-8.2; Adopt a pin injection to male and female parent population injection oxytocic drug luteotropin releasing hormone d-ala analog (LRH-A
2), dosage is 6 μ g/kg fish body weight.
(3) artificial insemination: the ovulation ejaculation situation checking male and female parent population after arriving Effect time, when raun have ovulation, milter has during ejaculation phenomenon and carries out artificial insemination, Insemination procedures is: first fish for and wait to produce raun, dry fish body moisture, got into by ovum in drying receptacle; Then fish for and wait to produce milter, dry gonopore, draw seminal fluid with syringe, put into spermatozoa diluent and mix with ovum again, then activate with clear water, leave standstill after 2 minutes, pour fertilized egg into incubator and hatch.
(4) hatch: in fertilized egg demoulding preincubation device, water volume flow rate is 0.4m/s, and after demoulding, flow velocity is 0.2m/s, control hatching water quality consistent with spawning inducing pool water quality; Seed rearing can be carried out after incubator is developed to the inflation of prelarva fish glue after demoulding.
In step (1), parent population Sex judging preferably by the following method: in mating season, ripe raun belly expands, and ovary profile is obvious, and gonopore reddens; Ripe milter pectoral fin has tiny pearl star, and feel is coarse, and light pressure belly has seminal fluid to flow out.Parent population maturity differentiates the method preferably adopting B ultrasonic and hand to squeeze, the feature that raun maturity is good: check parent population belly with B ultrasonic instrument, and ovary station diagram picture display particle is obvious, and proper alignment, fat-free mass between particle; The feature that milter maturity is good: light pressure parent population belly in water, have seminal fluid to flow out, and namely chance water falls apart.
Spermatozoa diluent described in step (3) is preferably 0.8%NaCl, and during insemination operation, the volume ratio of seminal fluid and spermatozoa diluent is preferably 1:5; Described incubator is preferably taper hatching pail or circular hatching channal.
The present invention has the following advantages and effect relative to prior art tool:
Instant invention overcomes that prior art uses that oxytocic drug kind and entry needle are time many, dosage greatly, the problem such as indefinite, the spawning rate of water quality index of hastening parturition and fertilization rate is low, technology poor repeatability.
The present invention makes to store up long Qi Wen Minnow artificial propagation and succeeds; its spawning rate is greater than 65%; fertilization rate is greater than 85%; incubation rate is greater than 80%; a large amount of prelarva can be provided for producing long Qi Wen Minnow size seedling seed in batches; long Qi Wen Minnow enhancement releasing is achieved, will plays an important role to protection Upper Yangtze River long Qi Wen Minnow resource.
Embodiment
Below in conjunction with embodiment, further detailed description is done to the present invention, but embodiments of the present invention are not limited thereto.
Embodiment 1
(1) parent population is selected: on April 18th, 2014 is selected to store up the anosis maturation without wound, physique stalwartness of 1-2 long fin kiss Minnow 15 tail as hastening parturition with parent population, wherein milter 9 tail, raun 6 tail from the Changjiang river aquatic products research institute cultivating workshop closed recirculating cultivating system.
Parent population Sex judging is by the following method: ripe raun belly expands, and ovary profile is obvious, and gonopore reddens; Ripe milter pectoral fin has tiny pearl star, and feel is coarse, and light pressure belly has seminal fluid to flow out.Parent population maturity differentiates the method adopting B ultrasonic and hand to squeeze, the feature that raun maturity is good: check parent population belly with B ultrasonic instrument, and ovary station diagram picture display particle is obvious, and proper alignment, fat-free mass between particle; The feature that milter maturity is good: light pressure parent population belly in water, have seminal fluid to flow out, and namely chance water falls apart.
(2) artificial induced spawning: the parent population of selection is put into 3m
2spawning inducing pool, pool inner water body keep miniflow, flow control is between 0.1-0.2m/s, and water temperature controls at 18-19 DEG C, and oxygen in water remains on more than 8mg/L, and pH is 7.4.Adopt a pin injection to male and female parent population injection oxytocic drug luteotropin releasing hormone d-ala analog (LRH-A
2), dosage is 6 μ g/kg fish body weight, and inject time is 5:30 afternoon on the 18th.
(3) artificial insemination: afternoon on the 19th, 2:00 checked, find that raun has ovulation, milter has ejaculation phenomenon, carry out artificial insemination immediately, Insemination procedures is: first fish for and wait to produce raun 2 tail, towel off dry fish body moisture, ovum is got in drying receptacle, then fish for and wait to produce milter 3 tail, dry gonopore, draw seminal fluid 1.5mL with syringe, put into 7.5mL spermatozoa diluent (0.8%NaCl), mix with ovum rapidly, then activate with clear water, leave standstill after 2 minutes, fertilized egg is poured into taper hatching pail and hatch.Parent population of all hastening parturition is divided and has operated for 3 times, and wherein 4 tail rauns produce entirely, and 2 tail rauns do not produce, spawning rate 66.7%, and 3.6 ten thousand, common property ovum obtains 3.1 ten thousand, fertilized egg, fertilization rate 86.1%.
(4) hatch: hatching adopts taper hatching pail, and inner bucket water rate of flow of fluid is 0.4m/s before fertilized egg demoulding, is 0.2m/s after demoulding, control hatching water quality consistent with spawning inducing pool water quality.After demoulding in hatching pail through the growth of 4 days, to prelarva fish glue inflation after remove hatching pail, 2.5 ten thousand tails of emerging altogether, incubation rate 80.6%.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (4)
1. a long fin kiss Minnow induced spawning method, is characterized in that comprising the steps:
(1) parent population is selected: select to store up more than the 1 year anosis maturation long Qi Wen Minnow without wound, physique stalwartness as hastening parturition with parent population from closed recirculating cultivating system, parent population female-male proportion is arranged in pairs or groups by 1:1.5;
(2) artificial induced spawning: the parent population of selection is put into spawning inducing pool, parent population is placed density and is less than 10 tails/m
2; Pool inner water body keeps miniflow, and flow control is between 0.1-0.2m/s, and water temperature controls at 16-19 DEG C, and oxygen in water remains on more than 8mg/L, and pH is between 7.0-8.2; Adopt a pin injection to male and female parent population injection oxytocic drug luteotropin releasing hormone d-ala analog, dosage is 6 μ g/kg fish body weight;
(3) artificial insemination: the ovulation ejaculation situation checking male and female parent population after arriving Effect time, when raun have ovulation, milter has during ejaculation phenomenon and carries out artificial insemination, Insemination procedures is: first fish for and wait to produce raun, dry fish body moisture, got into by ovum in drying receptacle; Then fish for and wait to produce milter, dry gonopore, draw seminal fluid with syringe, put into spermatozoa diluent and mix with ovum again, then activate with clear water, leave standstill after 2 minutes, pour fertilized egg into incubator and hatch;
(4) hatch: in fertilized egg demoulding preincubation device, water volume flow rate is 0.4m/s, and after demoulding, flow velocity is 0.2m/s, control hatching water quality consistent with spawning inducing pool water quality; Seed rearing can be carried out after incubator is developed to the inflation of prelarva fish glue after demoulding.
2. long Qi Wen Minnow induced spawning method according to claim 1, is characterized in that: in step (1), parent population Sex judging by the following method: in mating season, ripe raun belly expands, and ovary profile is obvious, and gonopore reddens; Ripe milter pectoral fin has tiny pearl star, and feel is coarse, and light pressure belly has seminal fluid to flow out; Parent population maturity differentiates the method adopting B ultrasonic and hand to squeeze, the feature that raun maturity is good: check parent population belly with B ultrasonic instrument, and ovary station diagram picture display particle is obvious, and proper alignment, fat-free mass between particle; The feature that milter maturity is good: light pressure parent population belly in water, have seminal fluid to flow out, and namely chance water falls apart.
3. long Qi Wen Minnow induced spawning method according to claim 1, is characterized in that: the spermatozoa diluent described in step (3) is 0.8%NaCl, and during insemination operation, the volume ratio of seminal fluid and spermatozoa diluent is 1:5.
4. long Qi Wen Minnow induced spawning method according to claim 1, is characterized in that: the incubator described in step (3) is taper hatching pail or circular hatching channal.
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| CN105284676B (en) * | 2015-09-24 | 2018-03-02 | 中国水产科学研究院长江水产研究所 | A kind of artificial culturing method of long Qi Wen Minnow parent populations |
| CN105284683A (en) * | 2015-10-26 | 2016-02-03 | 华中农业大学 | Artificial hatching method for Saurogobio dabryi |
| CN105409845B (en) * | 2015-12-01 | 2018-09-11 | 中国科学院昆明动物研究所 | A kind of short hairs schizothoracin artificial fecundation method |
| CN108513934A (en) * | 2018-02-27 | 2018-09-11 | 中国科学院南海海洋研究所 | A method of differentiating giant clam Oocyte maturity |
| CN110178761B (en) * | 2019-06-17 | 2021-10-29 | 江西省水产科学研究所 | Artificial spawning induction method for rhinogobio ventralis |
| CN115349468B (en) * | 2022-09-29 | 2024-03-01 | 福建省淡水水产研究所 | Full artificial breeding method for gobiogobiogobioides |
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