KR101465586B1 - Method for mass producing artificial seed of Abudefduf vaigiensis - Google Patents

Abstract

The present invention improves the survival rate and the hatching rate by stabilizing the process of hatching and hatching by propagating and defuncting the females and males, and activating the cultivation production of the frogs, And to provide a method for producing artificial seeds of Hapoori meat.

Description

[0001] Description [0002] METHOD FOR PRODUCING ARTIFICIAL SEEDS OF HEPPOURI MEAT [0002]

The present invention relates to a method for producing artificial seeds of Happosi meat, and more particularly, to a method for producing artificial seeds of Happori meat, and more particularly, The present invention relates to a method for producing artificial seedlings of Hapoori meat,

In general, it is called Abudefduf vaigiensis , which is called the Abudefduf bengalensis ), Abudefduf notatus ), black-tailed dome ( Abudefduf sexfasciatus ), a tethered dome ( Abudefduf sordidus ) There are five species, six species in Japan, and 13 species in the world.

These tropical and subtropical aquatic aquatic fishes have become an essential exhibit for other aquariums in the world. This is because it has a strong adaptability to the environment, and it is famous for its four lines of serpent fishes and five serpent fishes, and it has a very beautiful characteristic because it has yellow, blue, and black lines on its white background.

It is widely distributed in the tropical and subtropical regions of the world. It is distributed in Jeju Island in Korea, and it is inhabited to some parts of the southern coast of Korea due to the increase of temperature due to global warming.

However, in order to succeed in aquaculture in particular fish, the most important consideration should be the mating of the target species, provision of optimal scattering sites, stable hatching of embryos, and rearing of mullet. It is urgent to research and develop artificial seed production methods as described above in accordance with the necessity of development of new aquaculture varieties and ornamental fishes to prepare for tropical and subtropical fish species and to cope with climate change.

Patent No. 10-1037876 Patent No. 10-990093 Patent No. 10-764515 Patent No. 10-722339

Accordingly, it is an object of the present invention to provide a method for producing artificial seedlings of pineapple meat, as well as a method for mass production of pineapple meat.

Another object of the present invention is to develop and grow the ornamental fish industry through the artificial seedling of Happori meat, which has been impossible to produce artificial seeds due to the difficulty of artificial seedling in the meantime, and to provide the fishermen with high value-added ornamental fish form And to provide a method for producing artificial seedlings of Haporii meat that can increase income.

In order to achieve the above object, the present invention provides a method for producing artificial seedling of pineapple meat, comprising the steps of securing male and female pineapple meat at the same ratio and injecting them into a parent fish tank; Incubating the eggs generated from the male induction furnace in the above-mentioned autotrizing tank and the eggs produced in the incubation tank in a temperature range of 26 ° C to 29 ° C and 60% to 80% of sunlight; The incubated rabbits from the incubation tank were transferred to a tank and maintained at a temperature of 27 ° C to 29 ° C. The rabbits were fed for 3 days to 10 days from the initial feeding to the larvae immediately after hatching in 70% of sunlight (sunlight shining in the tank) And then feeding and breeding altemia; And finally feeding a large amount of food alone and growing the seedlings for seedling.

The weed harvesting method produced by the method according to the present invention enables the ornamental artificial seedling which has been dependent on the natural seedling during the time, and it is expected that the income increase effect will be provided to the fishermen through domestic and overseas exports, In addition, there is an effect that the ornamental fish industry can be intensively cultivated and the convenient and stable artificial seed production method can be improved.

1 is a photograph of the incubation process in the modification according to the present invention,
FIG. 2 is a photograph of the development process of the eardrum according to the present invention,
Fig. 3 is a photograph of mature Haporii meat.

Hereinafter, the present invention will be described in detail.

There are five species of hippopotamus such as hippopotamus, black rhododendron, rhododendron, black rhododendron, and tape rhododendron which live in Korea. As shown in FIG. 3, there is a strong environmental stress, four lines of serpentine and five lines of serpentine fish.

The method for producing artificial seeds of Haploiri meat according to the present invention comprises the following steps.

It is the step to secure the cancer and the number through the natural state or style. In other words, the female and male ratios were collected at a minimum of 15 ~ 20 grains per half of the female ratio, and scattering images (flat stones) and synthetic resin structures were installed in the fish tones, Clean up and guide the female.

That is, the male selects the appropriate rock mass and cleans the spout for about 30 to 40 hours to make a spawning image and attracts the female without going beyond a radius of 1m.

Then, when the male is attracted to the female, it is guided to the spawning phase and the female body is pierced with the mouth, thereby stimulating the female to breed.

When a female breeds, the males continue to form, but the males continue to attack and protect them so that no other individual can come close.

Spawning behavior continues for about 1 hour to 2 hours, and after the spawning is complete, the female leaves and the male keeps the eggs and continues to feed the fins with oxygen.

These scattered eggs usually have about 1,000 lips and about 3,500 lips in the case of the spotted eggs, and the eggs are scattered in a similar range. When the eggs are scattered, they are transferred to the hatcheries to induce hatching.

At this time, the water temperature of the incubation tank is 26 ℃ ~ 29 ℃ and the light intensity is 70% (the area where the sunlight shines in the water tank). Water quality stabilizer is added to improve water quality and a separate water injection device is installed.

Most of the hatching is within 2.5 hours after the hatching. The total length of the hatching is 2.55 ~ 2.86㎜, n (average 2.69㎜, n = 10, experimental flounder).

Then, when the hatching is completed, the water injection device is removed and the hatching process is performed.

The hatching process was as follows: at 6 hours after fertilization, the ovary had an oval shape and had a long diameter of 1.048 to 1.058 mm, a short n of 0.535 to 0.561 mm (average of 0.545 mm, n (average of 1.052 mm, = 10 birds, and experimental birds) reached 32 cell stage and had 3 ~ 6 eggs (Figure 1, A)

(Fig. 1, B) at 7 hours and 30 minutes after fertilization (Fig. 1, B), 8 hours and 30 minutes at blastocyst (Fig. 1, E).

At 15 hours after fertilization, the placenta was closed and an embryo started to form (Fig. 1F). At 21 hours 30 minutes, the head of the embryo developed (Fig. 1, G).

At 22 hours after fertilization, anophyll was formed in the embryo, a tail-side Kupper's vesicle was formed, and black vesicles appeared in the yolk (Fig. 1, H).

At 26 hours and 30 minutes after correction, the lens began to differentiate into the heart and eyes, 16-19 exfoliations were formed, and the number of eruptions decreased to one. At this time, agripped eggs with the head of the embryo facing the attachment side appeared (Fig. 1, I).

At 31 hours after fertilization, the number of black vesicles on the yolk and erosion increased to 20-24 (Fig. 1, J).

At the 33 hours and 30 minutes after fertilization, the ipsilateral nostrils began to differentiate and the heart beat started. As the black vesicles appeared in the body of the embryo, the yolk was separated from the tail and the dorsal fin was differentiated (Fig. 1, K).

At 39 hours and 30 minutes after fertilization, pigmentation began to appear in the eyes, and the tail became longer and had one large eternity. As the blood began to flow on the yolk, the embryos began to move, and the number of eradication increased to 28-30 (Fig. 1, L).

At the 46th hour after fertilization, pectoral fin and bladder began to differentiate, and black vesicles on the embryos and egg yolk increased.

At this time, the heart rate was 120 to 130 times per minute (Fig. 1, M)

At 47 hours and 30 minutes after correction, black vesicles were formed on the anus (Fig. 1, N).

At 53 hours after fertilization, the granulation hatching enzyme was secreted from the upper part of the head at a temperature of 25 ° C to 28 ° C (average 27 ° C), and the head part hatched toward the tip of the ovary in 54 hours (Fig.

[Development of the shape of the eagle]

The mouth and anus were open and the black parasites were scattered on the side of the boulevard, the anus, and the tail of the tail. .

All the fins were follicular, the anus was located in front of the center of the body, and the follicles were 20 ~ 23 (Fig. 2, A).

On the 3rd ~ 4th day after loading, the digestive tract was developed and began to eat small rotifer, which is the initial feed, from 2.74 to 2.97㎜ in total length and n (average 2.58㎜, n = 10,

The black vesicles were increased from the basal portion of the dorsal to the dorsal, and the umbilical cord was absorbed and connected to the digestive tract, and the pectoral fins developed on the membranous fins (Fig. 2, B).

On the 10th day after the loading, the late part of the head was 3.85 ~ 4.20㎜ in total length, and n (average 4.00㎜, n = 10, experimental marijuana).

The black vesicles were further increased around the bureau and the anus and the anus position was located in the center of the body (Fig. 2, C).

According to the present invention, it is preferable that the periphery of the pump of the water injecting apparatus is covered with a net and aerated so that dissolved oxygen is sufficient.

According to the present invention, as an initial feed after hatching, 120 to 350 rotifers (rotifers) are cultured at high density and fed until 10 days after hatching, and the feed rate is preferably 15 to 40 individuals / It is good to do it. If it is difficult to cultivate the rotifer, a method of supplying a freezing rotifer to reduce the cost is also worthy of study.

Next, from the 10th day after hatching, it is recommended to supply the alfalfa (large food) for about 3 ~ 5 days, reduce it initially, increase the supply quantity according to the growth, and supply 2 ~ 3 times / day.

And finally feeding the larvae to the fryer at a rate of about 10 times per day for up to 30 days.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples.

Example

(1) Preparation of mother

Happori breeding in aquariums and Happori meats, male and female, which had been mated and prepared for breeding, were used.

(2) Selection of the mother

A pair of infantile (20g body weight) whose abdomen was bloated and mated was selected.

(3) inputting a tone

One pair of female and male pairs were put into the tongue and the scattering phase and synthetic resin structure were installed.

The males were pruned for 30 ~ 40 hours in order to prepare for the spawning, and the females were induced in the range of 1m radius.

(4) scattering

Spawning behavior of Haporii meat continued for about 1 ~ 2 hours, and egg laying usually ranged from 1,000 ~ 3,500.

At this time, hatching occurs within about 100 hours at a water temperature of about 26 ° C.

When the egg is scattered, the water stabilizer is added to the scattering tank, and water is sprayed by a separate water injection device, and eggs are scattered in a good environment.

(5) Egg occurrence

At 6 hours after fertilization, the ovary was elliptically shaped and had a long diameter of 1.048 ~ 1.058㎜, n (mean 1.052㎜, n = 10, experimental number), 0.535 ~ 0.561㎜ in short diameter, Marly) reached 32 cell stage, 3-6 ovules and the egg is milky white, transparent, detached, attachable, attached by sticking yarn.

At 7 hours and 30 minutes after fertilization, 64 days were lost, 8 hours were lost, 8 hours and 30 minutes were blastocysts, and at 12 hours and 30 minutes, egg yolk was covered up and early vesicles were reached.

At 15 hours after fertilization, the placenta was closed and the embryo began to form. At 21 hours and 30 minutes, the head of the embryo developed.

At 22 hours after fertilization, anophyll was formed in the embryo, a tail-side Kupper's vesicle was formed, and black vesicles appeared in the yolk.

At 26 hours and 30 minutes after correction, the lens began to differentiate into the heart and eyes, 16-19 exfoliations were formed, and the number of eruptions decreased to one. At this time, eggs of agrippa eggs with the head of the embryo facing the attachment side appeared.

At 31 hours after fertilization, the number of black vesicles and extermination on egg yolk increased to 20 ~ 24.

At 33 hours and 30 minutes after fertilization, the efferent and nostril began to differentiate, heart beat began, and black vesicles appeared in the body of the embryo. The yolk of the tail parted and the dorsal fin was differentiated.

At 39 hours and 30 minutes after fertilization, pigmentation began to appear in the eyes, and the tail became longer and had one large eternity. As the blood began to flow on the yolk, the embryos began to move, and the number of eradication increased to 28 ~ 30.

At 46 hours after fertilization, the pectoral fin began to differentiate, and black vesicles on the embryos and yolk were increased.

At this time, the heart rate was 120 to 130 times per minute.

At 47 hours and 30 minutes after correction, black vesicles were formed on the anus.

At 53 hours after fertilization, the granulation hatching enzyme was secreted from the upper part of the head at 25 ℃ ~ 28 ℃ (average 27 ℃), and the head part hatches toward the tip of the ovary within 54 hours.

(6) Hatching and self-management

The mouth and anus were open and the black parasites were scattered on the side of the boulevard, the anus, and the tail of the tail. .

All fins were follicular, with the anus located in front of the center of the body, with 20-23 eradicates.

On the 3rd ~ 4th day after the loading, the digestive tract was developed and started to eat the rotifer which is the initial feed, 2.74 ~ 2.97㎜ in total length, n (average 2.58㎜, n = 10,

Black vesicles were increased from the basal portion of the dorsal to point - like, and the umbilical cord was absorbed and connected with the digestive tract, and the pectoral fin of the capsule developed into a fan shape.

On the 10th day after the loading, the late part of the head was 3.85 ~ 4.20㎜ in total length, and n (average 4.00㎜, n = 10, experimental marijuana).

Black vesicles increased further around the breech and the anus and the anus was located at the center of the body.

Claims (1)

In a method for producing artificial seedling of pineapple meat,
Securing the male and female breeders at the same rate,
In the above-mentioned hatching stage, male to female induction, incubation in a incubation tank of 60% to 80% (area where sunlight shines in the water tank) maintaining the water temperature of 26 ° C to 29 ° C and generating a cloud, ;
The incubated fish were transferred from the above incubation tank to a water tank and maintained at a temperature of 27 ° C to 29 ° C. After the incubation at 70% in the sunshine, small rotifers were fed for 3 days to 10 days as initial feed to the larvae. ; And
And finally feeding a large amount of food alone and growing the seed to a seedling for seedling production.

Images