CN101878741A - Interbreeding method of hemibarbus labeo and hemibarbus maculatus - Google Patents
Interbreeding method of hemibarbus labeo and hemibarbus maculatus Download PDFInfo
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- CN101878741A CN101878741A CN2010101537754A CN201010153775A CN101878741A CN 101878741 A CN101878741 A CN 101878741A CN 2010101537754 A CN2010101537754 A CN 2010101537754A CN 201010153775 A CN201010153775 A CN 201010153775A CN 101878741 A CN101878741 A CN 101878741A
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- lip
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- fry
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to an interbreeding method of hemibarbus labeo and hemibarbus maculates. In the interbreeding method, the hemibarbus labeo is selected as a female parent and the hemibarbus maculates is selected as a male parent, strengthened breeding is carried out, and technical measures, such as exogenous hormone maturing and induced spawning, running water irritation for spawn inducing, artificial dry fertilization, hatching process management, hybrid seedling seed breeding by adopting soybean milk and fish and shrimp pulverized granular feed and the like, are adopted so as to ensure that the induced spawning rate of a parent fish reaches more than 80 percent, the fertilization rate of spawns reaches more than 80 percent, and the hatching rate reaches more than 80 percent. The invention successfully provides the interbreeding method of the hemibarbus labeo and the hemibarbus maculates and breeds new fish breeding species with rapid growth and good meat quality for a fresh water aquiculture industry.
Description
Technical field
The invention belongs to the fish culture technical field, particularly relate to lip
With flower
Cross breeding method.
Background technology
Lip
(Hemibarbus labeo) is under the jurisdiction of Cypriniformes, Cyprinidae ， Minnow subfamily,
Belong to, be commonly called as that skin-carp meat, fiber crops are speared fish, the big drum fish.Mainly be distributed in the streams, rare in rivers, lake.Broke through lip from 2003
Artificial propagation techniques since, present lip
Mainly in pond and reservoir net cage, culture.
With lip
The flower that belongs to together
(Hemibarbus maculates) is commonly called as numb carp, fried dough twist fish, poplar bloassom fish, season youth fish etc.It is a kind of middle-size and small-size economic fish common in lake, the river.Calendar year 2001 is broken through flower
Artificial propagation techniques since, carry out the pond and raise together with mainly as the breed variety of taking of erythroculter ilishaeformis, simultaneously, flower
Also become inland waters and breed one of principal item of releasing.
Breed in recent years found that, female lip
Fast growth, male lip
Growth rate is slow, and spends
Just opposite, be male fast growth, female growth rate is slow, and there is tangible male and female difference in two kinds aspect growth rate.And in actual production, culture flower
Can not bring the economic benefit of expectation to the raiser, exist the phenomenon that germplasm is degenerated, lip
Meat is better than flower
, the market price is higher, and spends
Be more suitable for little aquifer cultivations such as pond.
The fish distant hybridization is one of basic means of fish breeding.If with lip
With flower
Carry out distant hybridization, promote lip
With flower
Between gene exchange, gene are reconfigured, be expected to cultivate the crossbreed that possesses parent both sides' merit or produce the unexistent new merit of parent, promptly obtain the good hybridization fish of fast growth, meat as culturing new varieties.So far do not see relevant lip as yet
With flower
The technology report of crossbreeding aspect.
Summary of the invention
The objective of the invention is to obtain a kind of lip rapid, that meat is good of growing
With flower
The propagation method of crossbreed.
The technical scheme of the inventive method comprises the following steps:
1., parent fish rearing, in breeding winter the previous year, select the healthy and strong lip of 2 ages in winter, body weight 0.1～0.3kg for use
As the hybridization female parent, select the flower of 2 ages in winter, body weight 0.1～0.25kg for use
As the hybridization male parent, be placed on respectively and carry out reinforced cultivating, lip in different ponds or the net cage
And flower
The manufactured feed of can throwing something and feeding in the winter time, can throw something and feed after the beginning of spring fish piece or fish gruel, having additional nutrients, and suitably bath;
2., hybridization hatching
A, adopt the exogenous hormone artificial induced spawning, when parent population breeding water body water temperature reaches 15 ℃, begin parent population is injected exogenous hormone, injecting the hormone position is the pectoral fin base portion, injects behind the hormone male parent flower
With maternal lip
Being placed in the same net cage current stimulation with flow velocity 0.1～0.2m/s impels and oestruses, ovulates;
B, artificial insemination were injected hormone after 15 hours, began to check, when light pressure lip
When belly has ovum to flow out, with lip
With flower
Separately, pick up flower earlier
, dry gonopore, squeeze a small amount of seminal fluid of sucking-off and splash in the dry container, and then pick up lip
, dry gonopore after, with lip
Ovum is clamp-oned in the said vesse, when clamp-oning 3～4 caudal lips
Behind the ovum, splash into flower again
Seminal fluid, so repeat to clamp-on seminal fluid and fish-egg, treat that fish-egg in the container has an appointment 200,000 the time, begin to use the slime water unsticking, fish-egg is slowly poured into slime water, stirred 1～2 minute, put into the hatching cylinder after rinsing is clean and hatch;
C, hatching management, mainly be to control the inflow of hatching cylinder well, anti-fish-egg deposition, prevent that the yarn tulle from stopping up, suitable increased water flow speed before and after the fry membrane reduces when fry can put down trip and hatches the interior water flow velocity of cylinder, behind the fry membrane 3～4 days, yolk sac absorbs and finishes, and can descend the pool to enter the fry rearing stage;
3., hybridization fry rearing
A, the mode that adopts the pond hydrostatic to cultivate, lip
With flower
The breeding density of hybridization fry about 150,000 tail/mus, 3～5 mu of pond areas for well, about the depth of water 0.8～1.0m, silt depth is less than 15cm at the bottom of the pond, it is convenient to advance draining;
B, bait feeding, begin to sprinkle soya-bean milk rich water under the fry behind the pool, one day secondary, sprinkling the slurry amount decides on pond water quality, when fry grows to the specification of 1.0 centimetres/tail, can throw something and feed through the fishes and shrimps particulate material of pulverizing, threw something and fed once in one day, this moment, also but timely and appropriate discovery added the part additive food, and additive food can be selected fish slurry, fish meal class for use;
C, water quality control, lip
With flower
The hybridization fry under before the pool to after the thorough disinfection of pond, pour into outer river clear water, the nurturing period, every about 10cm of pond water filling in 3～5 days, the nurturing period, Chi Shui is in the state of " fertile, alive, tender, refreshing ".
The ratio of choosing of described male parent parent population and maternal parent population is 1: 3.
The time of described artificial induced spawning and insemination, the natural temperature when hastening parturition will reach more than 15 ℃ mainly according to natural temperature and season and decide.
Described exogenous hormone is luteotropin releasing hormone d-ala analog LRH-A
2, human chorionic gonadtropin HCG and maleic acid DOM DOM hormotone, the raun application dose is LRH-A
210 μ g+HCG 1000IU+DOM 6mg/kg fish body weight, the milter application dose reduces by half.
The invention has the beneficial effects as follows: adopt that the exogenous hormone accelerating is hastened parturition, stimulation by running water is promoted production, technical measures such as artificial dry method hybridized insemination and hatching process management, make spawning rate, fertilization rate, the incubation rate of parent population all reach more than 80%; Hybrid seedlings adopts " soya-bean milk+fishes and shrimps pulverized particles feed " form to cultivate.The present invention successfully provides lip
With flower
Cross breeding method, for the fresh water culture fishery has been cultivated a kind of fast growth, breed new varieties that meat is good.
Embodiment
The invention will be further described with embodiment below.
Lip
With flower
Hybridizing propagation process, comprise the following steps:
1, parent fish rearing
The used parent population of present embodiment is collected maternal lip in the winter of breeding the previous year
From propagating the pond artificially, 2 ages in winter, the maximum 0.3kg/ tail of body weight, minimum 0.1kg/ tail, average weight 0.2kg/ tail; Male parent flower
From propagating the pond artificially, 2 ages in winter of the age of a fish, the maximum 0.25kg/ tail of body weight, minimum 0.1kg/ tail, average weight 0.2kg/ tail.Flower
And lip
Parent population be placed on respectively in 3 mu of ponds and cultivate, the nurturing period, the manufactured feed of throwing something and feeding in the winter of the previous year, reinforced cultivating is carried out in the combination of throw something and feed after the beginning of spring ice fresh fish piece, fish gruel and manufactured feed.
2, hybridization hatching
1., artificial induced spawning and insemination
A, parent fish selection, maternal parent population lip
Select: belly expands softness, and tangible ovary profile is arranged, and ventral groove is arranged, and gonopore is little red; Male parent parent population flower
Select: the inboard and rhynchodaenm of head, pectoral fin has significantly " pearl star " to occur, and hand has been touched coarse sensation, and gonopore is light color, gently squeezing belly adularescent seminal fluid, to flow out, meet water promptly diffusing.The ratio of choosing male parent parent population and maternal parent population is 1: 3.
The time of b, artificial induced spawning and insemination, mainly according to water temperature and mating season and decide, the water temperature when hastening parturition will reach more than 15 ℃, and the water temperature of hastening parturition in back 5 days remains on more than 15 ℃, so the time of this breeding is on April 12nd, 2009, water temperature is 18～21 ℃.
C, artificial induced spawning and insemination adopt artificial exogenous hormone to hasten parturition, and impel gonad maturity, ovulation, carry out artificial dry method insemination, the method that fertilized egg is hatched in the hatching cylinder.Hormone adopts luteotropin releasing hormone d-ala analog LRH-A
2, human chorionic gonadtropin HCG and maleic acid DOM DOM hormotone, the raun using dosage is LRH-A
210 μ g+HCG 1000IU+DOM 6mg/Kg fish body weight, the milter using dosage reduces by half.Better because of present embodiment parent population maturity, exogenous hormone adopts once and injects, and injects maternal lip earlier
, male parent flower reinjects
, will inject the male parent of exogenous hormone, maternal parent population then and be placed on that the current with flow velocity 0.1～0.2m/s stimulate in the same net cage, impel and oestrus, ovulate.
D, injection hormone were checked once every 1 hour after 15 hours, injected hormone after 19 hours, gently pressed lip
When belly has ovum to flow out, immediately with lip
With flower
Separately, carry out the dry method insemination.Pick up flower earlier
, dry gonopore, extrude a small amount of seminal fluid and splash in the dry container, and then pick up lip
, dry gonopore after, with lip
Ovum is clamp-oned in the said vesse, does not bring moisture content into container when squeezing the ovum operation, when clamp-oning 4 caudal lips
Behind the ovum, splash into flower again
Seminal fluid, so repeat to clamp-on seminal fluid and fish-egg, treat that fish-egg in the container has an appointment 200,000 the time, begin to use the slime water unsticking, fish-egg is slowly poured into slime water, stirred 1～2 minute, put into the hatching cylinder after rinsing is clean and hatch.Present embodiment is thrown in about 500,000 of hybridization fish-egg to the hatching cylinder altogether.
2., hatching management
The exomixis fish-egg is hatched in the circle hatching cylinder of diameter 0.8m, depth of water 1.0m, hatching water is the outer river that filters through the close screen cloth of 80 orders, pass through inlet valve, control well the hatching cylinder inflow, stirring so that the ovum during the embryonic development can all stir can not be too violent, emphasis is controlled the inflow of hatching cylinder well before and after the membrane, and prevents that the yarn tulle from stopping up.
3, hybridization fry rearing
The cultivation of present embodiment is carried out in the rectangle pond of 3 mu of areas, depth of water 1.5m, hybridization fry 34.8 ten thousand tails of putting in a suitable place to breed.
1. behind the fry membrane, note the inflow of control hatching cylinder, keep in the cylinder water quality pure and fresh, fry is supported after 4 days yolk sac temporarily and absorbs and finish, and promptly descend pool cultivation.
2. pond rearing
A, the mode that adopts the pond hydrostatic to cultivate, lip
With flower
The breeding density of hybridization fry be 11.6 ten thousand tail/mus, about depth of water 1.0m, silt depth is less than 15cm at the bottom of the pond, it is convenient to advance draining.
B, bait feeding begin behind the pool under the fry to sprinkle soya-bean milk rich water, use soya bean 2kg for every mu, and one day secondary when fry grows to the specification of 1 centimetre/tail, is thrown something and fed through the Penaeus Vannmei particulate material of pulverizing, and throws something and feeds once a day.
C, water quality control, lip
With flower
The hybridization fry under preceding 7 days of the pool, with 70kg/ mu quicklime to the pond thorough disinfection after, pour into outer river clear water.Nurturing period, note observing the activity of hybridization fish and the variation of pond water quality, allow Chi Shui be in the state of " fertile, alive, tender, refreshing " all the time.
The spawning rate of parent population is 89.2% in the present embodiment, and fertilization rate is 81.2%, incubation rate is 85.6%.
Claims (4)
1. lip
With flower
Hybridizing propagation process, it is characterized in that it comprises the following steps:
1. parent fish rearing is being bred winter the previous year, selects the healthy and strong lip of 2 ages in winter, body weight 0.1～0.3kg for use
As the hybridization female parent, select the flower of 2 ages in winter, body weight 0.1～0.25kg for use
As the hybridization male parent, be placed on respectively and carry out reinforced cultivating, lip in different ponds or the net cage
And flower
The manufactured feed of can throwing something and feeding in the winter time, can throw something and feed after the beginning of spring fish piece or fish gruel, having additional nutrients, and suitably bath;
2. hybridization hatching
A, adopt the exogenous hormone artificial induced spawning, when parent population breeding water body water temperature reaches 15 ℃, begin parent population is injected exogenous hormone, injecting the hormone position is the pectoral fin base portion, injects behind the hormone male parent flower
With maternal lip
Be placed in the same net cage current with flow velocity 0.1～0.2m/s and stimulate to impel and oestrus, ovulate;
B, artificial insemination were injected hormone after 15 hours, began to check, when light pressure lip
When belly has ovum to flow out, with lip
With flower
Separately, pick up flower earlier
, dry gonopore, squeeze a small amount of seminal fluid of sucking-off and splash in the dry container, and then pick up lip
, dry gonopore after, with lip
Ovum is clamp-oned in the said vesse, when clamp-oning 3～4 caudal lips
Behind the ovum, splash into flower again
Seminal fluid, so repeat to clamp-on seminal fluid and fish-egg, treat that fish-egg in the container has an appointment 200,000 the time, begin to use the slime water unsticking, fish-egg is slowly poured into slime water, stirred 1～2 minute, put into the hatching cylinder after rinsing is clean and hatch;
C, hatching management, mainly be to control the inflow of hatching cylinder well, anti-fish-egg deposition, prevent that the yarn tulle from stopping up, suitable increased water flow speed before and after the fry membrane reduces when fry can put down trip and hatches the interior water flow velocity of cylinder, behind the fry membrane 3～4 days, yolk sac absorbs and finishes, and can descend the pool to enter the fry rearing stage;
3. hybridize fry rearing
A, the mode that adopts the pond hydrostatic to cultivate, lip
With flower
The breeding density of hybridization fry about 150,000 tail/mus, 3～5 mu of pond areas for well, about the depth of water 0.8～1.0m, silt depth is less than 15cm at the bottom of the pond, it is convenient to advance draining;
B, bait feeding, begin to sprinkle soya-bean milk rich water under the fry behind the pool, one day secondary, sprinkling the slurry amount decides on pond water quality, when fry grows to the specification of 1.0 centimetres/tail, can throw something and feed through the fishes and shrimps particulate material of pulverizing, threw something and fed once in one day, this moment, also but timely and appropriate discovery added the part additive food, and additive food can be selected fish slurry, fish meal class for use;
C, water quality control, lip
With
The hybridization fry under before the pool to after the thorough disinfection of pond, pour into outer river clear water, the nurturing period, every about 10cm of pond water filling in 3～5 days, the nurturing period, Chi Shui is in the state of " fertile, alive, tender, refreshing ".
2. by the described lip of claim 1
With flower
Hybridizing propagation process, the ratio that it is characterized in that choosing male parent parent population and maternal parent population is 1: 3.
3. by the described lip of claim 1
With flower
Hybridizing propagation process, the time that it is characterized in that artificial induced spawning and insemination, the natural temperature when hastening parturition will reach more than 15 ℃ mainly according to natural temperature and season and decide.
4. by the described lip of claim 1
With flower
Hybridizing propagation process, it is characterized in that described exogenous hormone is luteotropin releasing hormone d-ala analog LRH-A
2, human chorionic gonadtropin HCG and maleic acid DOM DOM hormotone, the raun application dose is LRH-A
210 μ g+HCG 1000IU+DOM 6mg/Kg fish body weight, the milter application dose reduces by half.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101537754A CN101878741B (en) | 2010-04-17 | 2010-04-17 | Interbreeding method of hemibarbus labeo and hemibarbus maculatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101537754A CN101878741B (en) | 2010-04-17 | 2010-04-17 | Interbreeding method of hemibarbus labeo and hemibarbus maculatus |
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| CN101878741A true CN101878741A (en) | 2010-11-10 |
| CN101878741B CN101878741B (en) | 2012-05-02 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102228681A (en) * | 2011-06-09 | 2011-11-02 | 吴江市水产养殖有限公司 | Fish spawning inducer and spawning induction method |
| CN103404508A (en) * | 2013-06-19 | 2013-11-27 | 浙江省淡水水产研究所 | Ultra-low temperature cryopreservation and resuscitation method of hemibarbus maculates sperms |
| CN105724290A (en) * | 2016-03-02 | 2016-07-06 | 广西壮族自治区水产引育种中心 | Method for selecting red tilmpa parent directionally and constructing core group |
| CN106172119A (en) * | 2016-07-15 | 2016-12-07 | 天津现代晨辉科技集团有限公司 | Grass goldfish breeding and the method for selection-breeding |
| CN110036955A (en) * | 2019-05-31 | 2019-07-23 | 内江师范学院 | A kind of colored * and lip * first familiar generation seed propagation method |
| CN110150191A (en) * | 2019-06-28 | 2019-08-23 | 海南晨海水产有限公司 | A kind of artificial fecundation method of Plectorhinchus cinctus |
| CN110892874A (en) * | 2019-12-04 | 2020-03-20 | 成都市农林科学院 | Artificial cross breeding method for flowers and lips |
-
2010
- 2010-04-17 CN CN2010101537754A patent/CN101878741B/en not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
|---|
| 《水产养殖》 20030228 胡廷尖 花(鱼骨)生物学特性及人工繁养技术 , 第1期 2 * |
| 《水产科学》 20080331 姚子亮等 瓯江产唇(鱼骨)的早期形态发育研究 , 第3期 2 * |
| 《河北渔业》 20090731 胡廷尖等 花（鱼骨）提早人工催产及孵化试验 , 第7期 2 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102228681A (en) * | 2011-06-09 | 2011-11-02 | 吴江市水产养殖有限公司 | Fish spawning inducer and spawning induction method |
| CN103404508A (en) * | 2013-06-19 | 2013-11-27 | 浙江省淡水水产研究所 | Ultra-low temperature cryopreservation and resuscitation method of hemibarbus maculates sperms |
| CN103404508B (en) * | 2013-06-19 | 2014-12-24 | 浙江省淡水水产研究所 | Ultra-low temperature cryopreservation and resuscitation method of hemibarbus maculates sperms |
| CN105724290A (en) * | 2016-03-02 | 2016-07-06 | 广西壮族自治区水产引育种中心 | Method for selecting red tilmpa parent directionally and constructing core group |
| CN106172119A (en) * | 2016-07-15 | 2016-12-07 | 天津现代晨辉科技集团有限公司 | Grass goldfish breeding and the method for selection-breeding |
| CN110036955A (en) * | 2019-05-31 | 2019-07-23 | 内江师范学院 | A kind of colored * and lip * first familiar generation seed propagation method |
| CN110150191A (en) * | 2019-06-28 | 2019-08-23 | 海南晨海水产有限公司 | A kind of artificial fecundation method of Plectorhinchus cinctus |
| CN110150191B (en) * | 2019-06-28 | 2021-10-19 | 海南晨海水产有限公司 | Artificial propagation method of sparus fasciatus |
| CN110892874A (en) * | 2019-12-04 | 2020-03-20 | 成都市农林科学院 | Artificial cross breeding method for flowers and lips |
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| Publication number | Publication date |
|---|---|
| CN101878741B (en) | 2012-05-02 |
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