CN101878741B - Interbreeding method of hemibarbus labeo and hemibarbus maculatus - Google Patents

Interbreeding method of hemibarbus labeo and hemibarbus maculatus Download PDF

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Publication number
CN101878741B
CN101878741B CN2010101537754A CN201010153775A CN101878741B CN 101878741 B CN101878741 B CN 101878741B CN 2010101537754 A CN2010101537754 A CN 2010101537754A CN 201010153775 A CN201010153775 A CN 201010153775A CN 101878741 B CN101878741 B CN 101878741B
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China
Prior art keywords
fish
lip
flower
water
fry
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CN101878741A (en
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练青平
宓国强
胡廷尖
王雨辰
姚子亮
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Zhejiang Institute of Freshwater Fisheries
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Zhejiang Institute of Freshwater Fisheries
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

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Abstract

The invention relates to an interbreeding method of hemibarbus labeo and hemibarbus maculates. In the interbreeding method, the hemibarbus labeo is selected as a female parent and the hemibarbus maculates is selected as a male parent, strengthened breeding is carried out, and technical measures, such as exogenous hormone maturing and induced spawning, running water irritation for spawn inducing, artificial dry fertilization, hatching process management, hybrid seedling seed breeding by adopting soybean milk and fish and shrimp pulverized granular feed and the like, are adopted so as to ensure that the induced spawning rate of a parent fish reaches more than 80 percent, the fertilization rate of spawns reaches more than 80 percent, and the hatching rate reaches more than 80 percent. The invention successfully provides the interbreeding method of the hemibarbus labeo and the hemibarbus maculates and breeds new fish breeding species with rapid growth and good meat quality for a fresh water aquiculture industry.

Description

Lip * and the hybridizing propagation process of spending *
Technical field
The invention belongs to the fish culture technical field, particularly relate to lip
Figure GSB00000656293800013
and the cross breeding method of spending .
Background technology
Lip
Figure GSB00000656293800015
(Hemibarbus? labeo) belonging Cypriniformes, Cyprinidae, minnow subfamily, genus, commonly known as the heavy lip fish, flax fork fish, drum fish.Mainly be distributed in the streams, rare in rivers, lake.Since 2003 broke through the artificial propagation techniques of lip , lip
Figure GSB00000656293800018
was mainly cultured in pond and reservoir net cage at present.
The flower
Figure GSB000006562938000110
(Hemibarbus maculates) that belongs to together with lip
Figure GSB00000656293800019
is commonly called as numb carp; The fried dough twist fish; Poplar bloassom fish season youth fish etc.It is a kind of middle-size and small-size economic fish common in lake, the river.Calendar year 2001 has been broken through since the artificial propagation techniques of flower
Figure GSB000006562938000111
; The breed variety of taking mainly as erythroculter ilishaeformis carries out the pond and raises together with; Simultaneously, spend
Figure GSB000006562938000112
and also become one of principal item that inland waters propagation releases.
Breed result in recent years finds; Female lip
Figure GSB000006562938000113
fast growth; Male lip
Figure GSB000006562938000114
growth rate is slow; And spend
Figure GSB000006562938000115
just opposite; It is male fast growth; Female growth rate is slow, and there is tangible male and female difference in two kinds aspect growth rate.And in actual production; Culture flower
Figure GSB000006562938000116
and can not bring the economic benefit of expectation to the raiser; Exist the phenomenon that germplasm is degenerated; It is higher that lip meat is better than flower market price, and spend
Figure GSB000006562938000119
to be more suitable for little aquifer cultivations such as pond.
The fish distant hybridization is one of basic means of fish breeding.If lip
Figure GSB000006562938000120
and flower
Figure GSB000006562938000121
are carried out distant hybridization; Promote the gene exchange between lip
Figure GSB000006562938000122
and the flower
Figure GSB000006562938000123
; Gene are reconfigured; Be expected to cultivate the crossbreed that possesses parent both sides' merit or produce the unexistent new merit of parent, promptly obtain the good hybridization fish of fast growth, meat as culturing new varieties.Have yet to see on lip
Figure GSB000006562938000124
with flowers
Figure GSB000006562938000125
crossbreeding technical reports.
Summary of the invention
The present invention aims to obtain a rapid growth, good meat lip
Figure GSB000006562938000126
with flowers The hybrid breeding methods.
The technical scheme of the inventive method comprises the following steps:
1., parent fish rearing; Breeding winter the previous year; Select for use the healthy and strong lip
Figure GSB00000656293800021
of 2 ages in winter, body weight 0.1～0.3kg maternal as hybridization; The flower
Figure GSB00000656293800022
of selecting 2 ages in winter, body weight 0.1～0.25kg for use is as the hybridization male parent; Be placed on respectively and carry out reinforced cultivating in different ponds or the net cage; Lip
Figure GSB00000656293800023
and flower manufactured feed of throwing something and feeding in the winter time; Throw something and feed after beginning of spring fish piece or fish is rotten; Having additional nutrients, and suitably bath;
2., hybridization hatching
A, employing exogenous hormone artificial induced spawning; When parent population breeding water body water temperature reaches 15 ℃; Begin parent population is injected exogenous hormone; Injecting the hormone position is the pectoral fin base portion, injects behind the hormone male parent flower and maternal lip
Figure GSB00000656293800026
to be placed in the same net cage current with flow velocity 0.1～0.2m/s to irritate to impel and oestrus, ovulate;
B, artificial insemination; Inject hormone after 15 hours; Begin inspection; When light pressure lip belly has ovum to flow out; Lip
Figure GSB00000656293800028
and flower are separated; Pick up flower
Figure GSB000006562938000210
earlier and dry gonopore; Squeezing a small amount of seminal fluid of sucking-off splashes in the dry container; And then pick up after lip dries gonopore; Lip
Figure GSB000006562938000212
ovum is clamp-oned in the said vesse; After clamp-oning 3～4 caudal lips
Figure GSB000006562938000213
ovum; Splash into the seminal fluid of flower again; So repeat to clamp-on seminal fluid and fish-egg; Treat that fish-egg in the container has an appointment 200,000 the time; Begin to use the slime water unsticking; Slowly pour fish-egg into slime water; Stirred 1～2 minute, and put into the hatching cylinder after rinsing is clean and hatch;
C, hatching management mainly are to control the inflow of hatching cylinder well, anti-fish-egg deposition; Prevent that the yarn tulle from stopping up; Suitable increased water flow speed before and after the fry membrane reduces when fry can put down trip and hatches the interior water flow velocity of cylinder, behind the fry membrane 3～4 days; Yolk sac absorbs and finishes, and can descend the pool to get into the fry rearing stage;
3., hybridization fry rearing
A, the mode that adopts the pond hydrostatic to cultivate; The breeding density of the hybridization fry of lip
Figure GSB000006562938000215
and flower
Figure GSB000006562938000216
is about 150,000 tail/mus; 3～5 mu of pond areas for well, about the depth of water 0.8～1.0m; Silt depth advances draining conveniently less than 15cm at the bottom of the pond;
B, bait feeding begin to sprinkle soya-bean milk rich water, one day secondary behind the pool under the fry; Bold and vigorous slurry amount is looked pond water quality and is decided; When fry grows to the specification of 1.0 centimetres/tail, throw something and feed through the fishes and shrimps particulate material of pulverizing, threw something and fed once in one day; This moment, timely and appropriate discovery added the part additive food, and additive food is selected fish slurry, fish meal class for use;
C, water quality control; Under the hybridization fry of lip and flower
Figure GSB000006562938000218
before the pool to after the thorough disinfection of pond; Pour into outer river clear water; Nurturing period; Every at a distance from about 10cm of pond water filling in 3～5 days; Nurturing period, Chi Shui is in the state of " fertile, alive, tender, refreshing ".
The ratio of choosing of said male parent parent population and maternal parent population is 1: 3.
The time of said artificial induced spawning and insemination, the natural temperature when hastening parturition will reach more than 15 ℃ mainly according to natural temperature and season and decide.
Said exogenous hormone is luteotropin releasing hormone d-ala analog LRH-A 2, human chorionic gonadtropin HCG and maleic acid DOM DOM hormotone, the raun application dose is LRH-A 210 μ g+HCG 1000IU+DOM 6mg/Kg fish body weight, the milter application dose reduces by half.
The invention has the beneficial effects as follows: adopt that the exogenous hormone accelerating is hastened parturition, stimulation by running water is promoted production, technical measures such as artificial dry method hybridized insemination and hatching process management, make spawning rate, fertilization rate, the incubation rate of parent population all reach more than 80%; Hybrid seedlings adopts " soya-bean milk+fishes and shrimps pulverized particles feed " form to cultivate.The present invention successfully provides lip
Figure GSB00000656293800031
cross breeding method with flower
Figure GSB00000656293800032
, for the fresh water culture fishery has been cultivated a kind of fast growth, breed new varieties that meat is good.
Embodiment
With embodiment the present invention is described further below.
Lip
Figure GSB00000656293800033
and the hybridizing propagation process of spending
Figure GSB00000656293800034
comprise the following steps:
1, parent fish rearing
The used parent population of present embodiment was collected in the winter of breeding the previous year; Maternal lip
Figure GSB00000656293800035
is from propagating the pond artificially; 2 ages in winter; The maximum 0.3kg/ tail of body weight, minimum 0.1kg/ tail, average weight 0.2kg/ tail; Male parent flower is from propagating the pond artificially; 2 ages in winter of the age of a fish, the maximum 0.25kg/ tail of body weight; Minimum 0.1kg/ tail, average weight 0.2kg/ tail.The parent population of flower
Figure GSB00000656293800037
and lip
Figure GSB00000656293800038
is placed on respectively in 3 mu of ponds to be cultivated; Nurturing period; The manufactured feed of throwing something and feeding in the winter of the previous year, reinforced cultivating is carried out in the combination of throw something and feed after the beginning of spring ice fresh fish piece, fish gruel and manufactured feed.
2, hybridization hatching
1., artificial induced spawning and insemination
A, parent fish selection; Maternal parent population lip
Figure GSB00000656293800039
selects: belly expands softness; Tangible ovary profile is arranged; Ventral groove is arranged, and gonopore is little red; Selecting of male parent parent population flower
Figure GSB000006562938000310
: head, pectoral fin inboard and a rhynchodaenm have significantly " pearl star " to occur; Hand has been touched coarse sensation, and gonopore is light color, gently crowded belly adularescent seminal fluid outflow, chance water promptly loose.The ratio of choosing male parent parent population and maternal parent population is 1: 3.
The time of b, artificial induced spawning and insemination, mainly according to water temperature and mating season and decide, the water temperature when hastening parturition will reach more than 15 ℃, and the water temperature of hastening parturition in back 5 days remains on more than 15 ℃, so the time of this breeding is on April 12nd, 2009, water temperature is 18～21 ℃.
C, artificial induced spawning and insemination adopt artificial exogenous hormone to hasten parturition, and impel gonad maturity, ovulation, carry out artificial dry method insemination, the method that fertilized egg is hatched in the hatching cylinder.Hormone adopts luteotropin releasing hormone d-ala analog LRH-A 2, human chorionic gonadtropin HCG and maleic acid DOM DOM hormotone, the raun using dosage is LRH-A 210 μ g+HCG 1000IU+DOM 6mg/Kg fish body weight, the milter using dosage reduces by half.Because of present embodiment parent population maturity better; Exogenous hormone adopts once and injects; Injection maternal lip
Figure GSB00000656293800041
male parent flower that reinjects
Figure GSB00000656293800042
will inject the male parent of exogenous hormone then earlier, maternal parent population is placed on the interior current with flow velocity 0.1～0.2m/s of same net cage and irritates, and impel and oestrus, ovulates.
D, injection hormone are after 15 hours; Whenever once at a distance from inspection in 1 hour; Inject hormone after 19 hours; When light pressure lip
Figure GSB00000656293800043
belly has ovum to flow out; Immediately lip
Figure GSB00000656293800044
and flower are separated, carry out the dry method insemination.Pick up flower
Figure GSB00000656293800046
earlier and dry gonopore; Extruding a small amount of seminal fluid splashes in the dry container; And then pick up after lip dries gonopore; Lip
Figure GSB00000656293800048
ovum is clamp-oned in the said vesse; Do not bring moisture content into container when squeezing the ovum operation; After clamp-oning 4 caudal lips
Figure GSB00000656293800049
ovum; Splash into the seminal fluid of flower
Figure GSB000006562938000410
again; So repeat to clamp-on seminal fluid and fish-egg; Treat that fish-egg in the container has an appointment 200,000 the time; Begin to use the slime water unsticking; Slowly pour fish-egg into slime water, stirred 1～2 minute, put into the hatching cylinder after rinsing is clean and hatch.Present embodiment is thrown in about 500,000 of hybridization fish-egg to the hatching cylinder altogether.
2., hatching management
The exomixis fish-egg is hatched in the circle hatching cylinder of diameter 0.8m, depth of water 1.0m; Hatching water be the outer river that filters through the close screen cloth of 80 orders, through inlet valve, controls the inflow of hatching cylinder well; So that the ovum during the embryonic development can all stir can not be too violent; Emphasis is controlled the inflow of hatching cylinder well before and after the membrane, and prevents that the yarn tulle from stopping up.
3, hybridization fry rearing
The cultivation of present embodiment is carried out in the rectangle pond of 3 mu of areas, depth of water 1.5m, hybridization fry 34.8 ten thousand tails of putting in a suitable place to breed.
1. behind the fry membrane, note the inflow of control hatching cylinder, keep in the cylinder water quality pure and fresh, fry is supported after 4 days yolk sac temporarily and absorbs and finish, and promptly descend pool cultivation.
2. pond rearing
A, the mode that adopts the pond hydrostatic to cultivate; Lip
Figure GSB00000656293800051
is 11.6 ten thousand tail/mus with the breeding density of the hybridization fry of flower
Figure GSB00000656293800052
; About depth of water 1.0m; Silt depth advances draining conveniently less than 15cm at the bottom of the pond.
B, bait feeding begin behind the pool under the fry to sprinkle soya-bean milk rich water, use soya bean 2kg for every mu, and one day secondary when fry grows to the specification of 1 centimetre/tail, is thrown something and fed through the Penaeus Vannmei particulate material of pulverizing, and throws something and feeds once a day.
C, water quality control; The pool is preceding 7 days under the hybridization fry of lip
Figure GSB00000656293800053
and flower
Figure GSB00000656293800054
; With 70kg/ mu quicklime to the pond thorough disinfection after, pour into outer river clear water.Nurturing period, note observing the activity of hybridization fish and the variation of pond water quality, let Chi Shui be in the state of " fertile, alive, tender, refreshing " all the time.
The spawning rate of parent population is 89.2% in the present embodiment, and fertilization rate is 81.2%, incubation rate is 85.6%.

Claims (4)

1. a lip
Figure FSB00000656293700011
and the hybridizing propagation process of spending
Figure FSB00000656293700012
is characterized in that it comprises the following steps:
1. parent fish rearing; Breeding winter the previous year; Select for use the healthy and strong lip
Figure FSB00000656293700013
of 2 ages in winter, body weight 0.1～0.3kg maternal as hybridization; The flower
Figure FSB00000656293700014
of selecting 2 ages in winter, body weight 0.1～0.25kg for use is as the hybridization male parent; Be placed on respectively and carry out reinforced cultivating in different ponds or the net cage; Lip
Figure FSB00000656293700015
and flower
Figure FSB00000656293700016
manufactured feed of throwing something and feeding in the winter time; Throw something and feed after beginning of spring fish piece or fish is rotten; Having additional nutrients, and suitably bath;
2. hybridization hatching
A, employing exogenous hormone artificial induced spawning; When parent population breeding water body water temperature reaches 15 ℃; Begin parent population is injected exogenous hormone; Injecting the hormone position is the pectoral fin base portion, inject behind the hormone with male parent flower
Figure FSB00000656293700017
and maternal lip
Figure FSB00000656293700018
be placed in the same net cage current with flow velocity 0.1～0.2m/s and irritate to impel and oestrus, ovulate;
B, artificial insemination; Inject hormone after 15 hours; Begin inspection; When light pressure lip
Figure FSB00000656293700019
belly has ovum to flow out; Lip and flower
Figure FSB000006562937000111
are separated; Pick up flower
Figure FSB000006562937000112
earlier and dry gonopore; Squeezing a small amount of seminal fluid of sucking-off splashes in the dry container; And then pick up after lip
Figure FSB000006562937000113
dries gonopore; Lip
Figure FSB000006562937000114
ovum is clamp-oned in the said vesse; After clamp-oning 3～4 caudal lips
Figure FSB000006562937000115
ovum; Splash into the seminal fluid of flower
Figure FSB000006562937000116
again; So repeat to clamp-on seminal fluid and fish-egg; Treat that fish-egg in the container has an appointment 200,000 the time; Begin to use the slime water unsticking; Slowly pour fish-egg into slime water; Stirred 1～2 minute, and put into the hatching cylinder after rinsing is clean and hatch;
C, hatching management mainly are to control the inflow of hatching cylinder well, anti-fish-egg deposition; Prevent that the yarn tulle from stopping up; Suitable increased water flow speed before and after the fry membrane reduces when fry can put down trip and hatches the interior water flow velocity of cylinder, behind the fry membrane 3～4 days; Yolk sac absorbs and finishes, and can descend the pool to get into the fry rearing stage;
3. hybridize fry rearing
A, the mode that adopts the pond hydrostatic to cultivate; The breeding density of the hybridization fry of lip
Figure FSB000006562937000117
and flower
Figure FSB000006562937000118
is about 150,000 tail/mus; 3～5 mu of pond areas for well, about the depth of water 0.8～1.0m; Silt depth advances draining conveniently less than 15cm at the bottom of the pond;
B, bait feeding begin to sprinkle soya-bean milk rich water, one day secondary behind the pool under the fry; Bold and vigorous slurry amount is looked pond water quality and is decided; When fry grows to the specification of 1.0 centimetres/tail, throw something and feed through the fishes and shrimps particulate material of pulverizing, threw something and fed once in one day; This moment, timely and appropriate discovery added the part additive food, and additive food is selected fish slurry, fish meal class for use;
C, water quality control; Under the hybridization fry of lip
Figure FSB00000656293700021
and flower
Figure FSB00000656293700022
before the pool to after the thorough disinfection of pond; Pour into outer river clear water; Nurturing period; Every at a distance from about 10cm of pond water filling in 3～5 days; Nurturing period, Chi Shui is in the state of " fertile, alive, tender, refreshing ".
2. by the hybridizing propagation process of the described lip of claim 1
Figure FSB00000656293700023
with flower
Figure FSB00000656293700024
, the ratio that it is characterized in that choosing male parent parent population and maternal parent population is 1: 3.
3. by the hybridizing propagation process of the described lip of claim 1 with flower ; The time that it is characterized in that artificial induced spawning and insemination, the natural temperature when hastening parturition will reach more than 15 ℃ mainly according to natural temperature and season and decide.
4. by the described lip of claim 1
Figure FSB00000656293700027
With flower
Figure FSB00000656293700028
Hybridizing propagation process, it is characterized in that said exogenous hormone is luteotropin releasing hormone d-ala analog LRH-A 2, human chorionic gonadtropin HCG and maleic acid DOM DOM hormotone, the raun application dose is LRH-A 210 μ g+HCG 1000IU+DOM 6mg/Kg fish body weight, the milter application dose reduces by half.
CN2010101537754A 2010-04-17 2010-04-17 Interbreeding method of hemibarbus labeo and hemibarbus maculatus Expired - Fee Related CN101878741B (en)

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Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102228681A (en) * 2011-06-09 2011-11-02 吴江市水产养殖有限公司 Fish spawning inducer and spawning induction method
CN103404508B (en) * 2013-06-19 2014-12-24 浙江省淡水水产研究所 Ultra-low temperature cryopreservation and resuscitation method of hemibarbus maculates sperms
CN105724290B (en) * 2016-03-02 2021-05-18 广西壮族自治区水产引育种中心 Method for directionally selecting red tilapia parents and establishing core group
CN106172119A (en) * 2016-07-15 2016-12-07 天津现代晨辉科技集团有限公司 Grass goldfish breeding and the method for selection-breeding
CN110036955A (en) * 2019-05-31 2019-07-23 内江师范学院 A kind of colored * and lip * first familiar generation seed propagation method
CN110150191B (en) * 2019-06-28 2021-10-19 海南晨海水产有限公司 Artificial propagation method of sparus fasciatus
CN110892874A (en) * 2019-12-04 2020-03-20 成都市农林科学院 Artificial cross breeding method for flowers and lips

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