CN110036955A - A kind of colored * and lip * first familiar generation seed propagation method - Google Patents
A kind of colored * and lip * first familiar generation seed propagation method Download PDFInfo
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- CN110036955A CN110036955A CN201910468988.7A CN201910468988A CN110036955A CN 110036955 A CN110036955 A CN 110036955A CN 201910468988 A CN201910468988 A CN 201910468988A CN 110036955 A CN110036955 A CN 110036955A
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- propagation method
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The present invention provides a kind of flowersWith lipFirst familiar generation seed propagation method, this method is with flowerAs female parent, with lipAs male parent, cultivate by controlling, hasten parturition, hatch etc. the Parameter Conditions of processes, parent population maturing rate is realized up to 75% or more, and maturation time is neat, fish brood amount is high, semen quality is good, parent population egg-laying time is consistent, fertility rate and hatchability reach 90% or more technical effect.The present invention can be used for first familiar generation
Description
Technical field
The present invention relates to hybridization technique fields, and in particular to Yi ZhonghuaWith lipFirst familiar generation seed propagation method.
Background technique
FlowerAnd lipBelong to Cypriniformes, Cyprinidae, Minnow subfamily,Belong to.It is distributed in the major rivers in China, lake, reservoir
In, it is a kind of higher middle-size and small-size fish of economic value.Its egg-laying season is the first tenday period of a month in early April to May, belongs to disposable breeding fish
Class.Demersal egg, viscosity are high, and ovum is in pale or faint yellow, and fertilized eggs last 26~29h hatching at 19-24 DEG C or so.In recent years
Come, the flower of artificial breedingAnd lipOxygen demand is high, irritability is strong and is not easy to catch, and constrains its cultured area and promotes speed
Degree.
Currently, how to solve to spendAnd lipIn terms of the disadvantage that oxygen demand is high, irritability is strong, not yet discovery effect compared with
Good method.Although hybridization technique has a wide range of applications at aspect of cultivating fine seed strains, for the female parent of hybridization, parent, hybridization
The selection of process has very high requirement, is otherwise difficult to obtain compared with parent characteristic more preferably cenospecies.
Summary of the invention
In view of the shortcomings of the prior art and the market demand, it is an object of the invention to be artificial large-scale cultivation (such as pond
The comprehensive breeding of pool cultivation and rice field) propagation method of high-quality seed is provided, the parent population sexual gland maturing rate that this method is cultivated is high, mature
Degree is neat, semen quality is good, parent population egg-laying time is neat, rate of fertilization is high, hatchability of fertile eggs is high；The gained under the method for the present invention
First familiar generation seed has the speed of growth fast, and disease resistance is strong, and disposition is docile, and oxygen demand is lower than good characteristics such as parents.
To achieve the goals above, the solution of the present invention is as follows:
(1) flower is chosenAs female parent, lipAs male parent, control female-male proportion is 2~3:1；
(2) step (1) described object is placed in parent fish rearing system, cultivate when, control parent population cultivation density be 10~
15 tails/m3；Controlling water temperature is 15~25 DEG C；The water quality dissolved oxygen of breeding water is controlled in 8mg/L or more, ammonia-nitrogen content exists
0.01mg/L or less；During 2 month of December the previous year to next year, feeds within every two days and once (thrown when such as every two day noon 12
Feed primary), respectively feed sooner or later daily in remaining month it is primary (as daily early 7 when, respectively feed when evening 18 it is primary), the feed fed
Protein content be 38~40%, partial size be 3.0~5.0mm；
(3) it is hastened parturition using drug human chorionic gonadtropin and luteotropin releasing hormone d-ala analog；For raun
For, the dosage of human chorionic gonadtropin (abbreviation HCG) and luteotropin releasing hormone d-ala analog (abbreviation LRH-A2)
Respectively 1~1.5 μ g/ tail and 800~1200IU/ tail；For milter, human chorionic gonadtropin and luteotropin release
The dosage of hormone analogs is respectively 0~0.5 μ g/ tail and 0~400IU/ tail；
(4) after carrying out step (3), artificial insemination is carried out, and hatched.
As optional technical solution, in practical reproductive process, the flowerAnd lipIt is a to be cultivated after artificial propagation
Body, the long range of body is 15~25cm, weight is 180~220g.
As optional technical solution, the parent fish rearing system is indoor stereo circulating water culture system.
Under normal circumstances, the breeding water be by filtering, plant is denitrogenated, ultraviolet-sterilization is handled and can be recycled
's.
As a preferred technical solution, in step (1), flower is chosenAnd lipTime be early April.
In practical reproductive process, when being hastened parturition, chooses abdomen and expand raun and figure stalwartness milter, the chorion
Promoting sexual gland hormone and luteotropin releasing hormone d-ala analog are one injection, and breeding water temperature when hastening parturition is 19~24 DEG C, effect
Time is 14~20h.
In practical reproductive process, milter can squeeze out milky, thick sperm, human chorionic gonadtropin and promote corpus luteum
The dosage of hormone-releasing hormone analog is 0.For the milter of lack of quantity of spermatic fluid, human chorionic gonadtropin and luteotropin
The dosage of releasing hormone analog is respectively 0.5 μ g and 400IU.
As a kind of optional technical solution, when carrying out artificial insemination, what is taken is artificial dry method fertilization.
As a kind of optional technical solution, when being hatched, carried out using hatching net.
When being hatched as a preferred technical solution, water temperature is 19~24 DEG C, and brooding time is 26~29h, is hatched
Timely adjustment water flow size and fry membrane situation is observed in journey, hatching net must be taken out before putting down trip after fry membrane.
Beneficial effects of the present invention:
The present invention is using flowerAs maternal, lipAs male parent carry out parent fish rearing, make parent population maturing rate up to 75% with
On, and maturation time is neat, fish brood amount is high, semen quality is good.The present invention carries out artificial induced spawning, parent population using HCG and LRH-A2
Egg-laying time is consistent；Fertility rate and hatchability of the invention simultaneously reaches 90% or more.
The present invention can be used for first familiar generation (flower) interior pool artificial breeding, for F1 generation large-scale artificial
The comprehensive breeding of cultivation and rice field has important directive significance.
Specific embodiment
The present invention is specifically described below by embodiment, it is necessary to which indicated herein is that following embodiment is only used
It is further detailed in the present invention, should not be understood as limiting the scope of the invention, which is skilled in technique
Some nonessential modifications and adaptations that personnel are made according to foregoing invention content, still fall within protection scope of the present invention.
♀ represents female parent in following embodiment, and ♂ represents male parent.
Embodiment 1
The flower of the present embodimentWith lipFirst familiar generation seed propagation method, using following steps:
1) parent fish rearing
Parent fish rearing in July, 2015 flowerLipCirculation is entered the room to start to cultivate.
Parent population source: the flower of artificial propagation raisingLipParent population, the long range of body are 15~25cm, body
Focus on the cultivation individual of 180~220g.
Male and female match: Ci ︰ hero=2~3 ︰ 1.
Parent fish rearing pond: using (15 DEG C~25 DEG C) of indoor circulating water cultivation system natural temperature cultivations.
Water-quality control: excrement is removed by filtration in breeding water and residual bait, plant denitrogenate, are recycled after ultraviolet-sterilization, and
New water is supplemented in right amount.Cultivation water dissolved oxygen is controlled in 8mg/L or more, and ammonia control is in 0.01mg/L or less.
Cultivation density: 10~15 tails of parent population cultivation density/m3。
Bait feeding: feed artifical compound feed (thick protein 38.0~40.0%, crude fibre≤5.0%, crude fat >=
3.5%, total phosphorus >=1.0%, moisture≤12.5%, lysine >=1.85%), fed when every two day noon 12 at the beginning of December~2 month
Once, remaining month daily early 7 when, when evening 18, respectively feeds primary, is had enough with 80% fish as principle.
2) the artificial induced spawning selection of parent population
In late March, 2015-early April first checks for milter gonad development situation, when milter can squeeze out sperm, chooses
Abdomen is selected to expand raun and figure stalwartness milter for artificial induced spawning.
3) artificial induced spawning
Oxytocic drug is HCG and LRH-A2.Raun dosage of hastening parturition is 1~1.5 μ g LRH-A2 of every tail fish, 800~
1200IUHCG, milter can squeeze out milky, thick sperm person does not urge, and lack of quantity of spermatic fluid person hastens parturition dosage as every 0.5 μ g of tail fish
LRH-A2,400IU HCG.Dosage of hastening parturition is adjusted depending on fish body size and time of hastening parturition, when fish body is larger, the time of hastening parturition is more early
Dosage is larger.One injection.Injection system is pectoral fin base portion.19 DEG C~24 DEG C of water temperature of breeding.Effect time is 14~20h.
4) ovum fertilization is adopted
Artificial dry method insemination.Specific method is: firstly, milter is pulled out, encasing head with clean wet towel, uses thumb
It is gently squeezed along abdomen to gonopore direction, squeezes out sperm, it is spare after microscopy is qualified；Later, female parent population is pulled out, with clean wet
Towel encases head, then gently squeezes fish belly two sides to gonopore direction, ovum is smoothly squeezed out；Later, essence is added into ovum rapidly
Liquid, feather stir evenly, and add aeration tap water activation, 10~20s fertilization；After carry out artificial incubation.
5) hatch
Hatching net hatching.Specific method is: fertilized eggs paving being spread across hatching net, long × wide (1 × 0.5m) hatching net can incubate
Change fertilized eggs 50,000；Water inlet switch is opened, water flow size is adjusted, is advisable with floating do not sink to the bottom in water of hatching net；Fry hatches
Afterwards, turn off water inlet switch, by hatching net removal, carry out fry rearing.Carried out disinfection to hatching system before hatching with bleaching powder and
Rinsing, Spawning water are aeration tap water, are changed without new water in hatching process.At 19 DEG C~24 DEG C of water temperature, 26~29h can be incubated
Dissolve prelarva.
The present invention is spent using measures such as control breeding density, adjustment bait, recirculated water running water technologiesLipParent fish rearing makes parent population maturing rate up to 75% or more, and maturation time is neat, fish brood amount is high, semen quality is good.Fortune
Artificial induced spawning is carried out with HCG and LRH-A2, parent population egg-laying time is consistent, the insemination of artificial dry method, hatching net hatching, rate of fertilization and incubates
Rate reaches 90% or more.The present invention can be for first familiar generation (flower× lip ) scale artificial culture
It is important with important directive significance with the comprehensive breeding in rice field.
Claims (10)
1. a kind of flowerWith lipFirst familiar generation seed propagation method, which is characterized in that the propagation method includes the following steps:
(1) flower is chosenAs female parent, lipAs male parent, control female-male proportion is 2~3:1；
(2) step (1) described object is placed in parent fish rearing system, cultivate when, control parent population cultivation density for 10~15 tails/
m3；Controlling water temperature is 15~25 DEG C；The water quality dissolved oxygen of breeding water is controlled in 8mg/L or more, ammonia-nitrogen content 0.01mg/L with
Under；During 2 months in December to the next year of the previous year, feed within every two days once, remaining month respectively feeds one sooner or later daily
Secondary, the protein content of the feed fed is 38~40%, and partial size is 3.0~5.0mm；
(3) it is hastened parturition using drug human chorionic gonadtropin and luteotropin releasing hormone d-ala analog；For raun,
The dosage of human chorionic gonadtropin and luteotropin releasing hormone d-ala analog be respectively 1~1.5 μ g/ tail and 800~
1200IU/ tail；For milter, the dosage of human chorionic gonadtropin and luteotropin releasing hormone d-ala analog is distinguished
For 0~0.5 μ g/ tail and 0~400IU/ tail；
(4) after carrying out step (3), artificial insemination is carried out, and hatched.
2. propagation method according to claim 1, which is characterized in that the flowerAnd lipManually to be supported after artificial propagation
Individual is grown, the long range of body is 15~25cm, weight is 180~220g.
3. propagation method according to claim 1, which is characterized in that the parent fish rearing system is indoor stereo recirculated water
Cultivating system.
4. propagation method according to claim 1, which is characterized in that the breeding water be by filtering, plant denitrogenate,
Ultraviolet-sterilization is handled and be can be recycled.
5. propagation method according to claim 1, which is characterized in that in step (1), choose flowerAnd lipBreeding when
Between be early April.
6. propagation method according to claim 1, which is characterized in that when being hastened parturition, choose abdomen and expand raun and body
Type stalwartness milter, the human chorionic gonadtropin and luteotropin releasing hormone d-ala analog are one injection, numerous when hastening parturition
Growing water temperature is 19~24 DEG C, and Effect time is 14~20h.
7. propagation method according to claim 1 or 6, which is characterized in that milter can squeeze out milky, thick sperm,
The dosage of human chorionic gonadtropin and luteotropin releasing hormone d-ala analog is 0.
8. propagation method according to claim 1, which is characterized in that when carrying out artificial insemination, what is taken is artificial dry method
Fertilization.
9. propagation method according to claim 1, which is characterized in that when being hatched, carried out using hatching net.
10. propagation method according to claim 8, which is characterized in that when being hatched, water temperature is 19~24 DEG C, hatching
Time is 26~29h, timely adjustment circulating water flow size and observes fry membrane situation in hatching process, trip is put down after fry membrane
Before must take out hatching net.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910468988.7A CN110036955A (en) | 2019-05-31 | 2019-05-31 | A kind of colored * and lip * first familiar generation seed propagation method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910468988.7A CN110036955A (en) | 2019-05-31 | 2019-05-31 | A kind of colored * and lip * first familiar generation seed propagation method |
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| CN110036955A true CN110036955A (en) | 2019-07-23 |
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| CN201910468988.7A Pending CN110036955A (en) | 2019-05-31 | 2019-05-31 | A kind of colored * and lip * first familiar generation seed propagation method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110892874A (en) * | 2019-12-04 | 2020-03-20 | 成都市农林科学院 | Artificial cross breeding method for flowers and lips |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101878741A (en) * | 2010-04-17 | 2010-11-10 | 浙江省淡水水产研究所 | Interbreeding method of hemibarbus labeo and hemibarbus maculatus |
| CN103404508A (en) * | 2013-06-19 | 2013-11-27 | 浙江省淡水水产研究所 | Ultra-low temperature cryopreservation and resuscitation method of hemibarbus maculates sperms |
| CN103444611A (en) * | 2013-09-29 | 2013-12-18 | 四川省农业科学院水产研究所 | Artificial propagation method of Gymnodiptychus pachycheilus |
| CN104304103A (en) * | 2014-09-17 | 2015-01-28 | 丹东市水产技术推广总站 | Seedling breeding method for early breeding and cultivating large-size hemibarbus labeo |
-
2019
- 2019-05-31 CN CN201910468988.7A patent/CN110036955A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101878741A (en) * | 2010-04-17 | 2010-11-10 | 浙江省淡水水产研究所 | Interbreeding method of hemibarbus labeo and hemibarbus maculatus |
| CN103404508A (en) * | 2013-06-19 | 2013-11-27 | 浙江省淡水水产研究所 | Ultra-low temperature cryopreservation and resuscitation method of hemibarbus maculates sperms |
| CN103444611A (en) * | 2013-09-29 | 2013-12-18 | 四川省农业科学院水产研究所 | Artificial propagation method of Gymnodiptychus pachycheilus |
| CN104304103A (en) * | 2014-09-17 | 2015-01-28 | 丹东市水产技术推广总站 | Seedling breeding method for early breeding and cultivating large-size hemibarbus labeo |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110892874A (en) * | 2019-12-04 | 2020-03-20 | 成都市农林科学院 | Artificial cross breeding method for flowers and lips |
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Application publication date: 20190723 |