CN103636543A - Indoor artificial breeding method of glossogobius giuris - Google Patents
Indoor artificial breeding method of glossogobius giuris Download PDFInfo
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- CN103636543A CN103636543A CN201310696637.4A CN201310696637A CN103636543A CN 103636543 A CN103636543 A CN 103636543A CN 201310696637 A CN201310696637 A CN 201310696637A CN 103636543 A CN103636543 A CN 103636543A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
An indoor artificial breeding method of glossogobius giuris comprises the steps of indoor intensive breeding of parent glossogobius giuris, artificial ripening and hastening, artificial hatching and offspring seedling breeding. The indoor artificial breeding method is characterized in that after intensive overwintering breeding of the glossogobius giuris, the artificial hastening is performed, a lutropin releasing hormone A2 and a human chorionic gonadotropin are used as hastening hormones, after ripening and hastening of male and female parent glossogobius giuris, zygotes adhere to lining meshes of laying nests, the meshes to which the glossogobius giuris zygotes adhere are taken out, unfolded evenly and vertically hung in a hatching pond, after release, rich water seedling culture is adopted for primarily hatching yellow seedlings, after seawater rotifers are replaced by artemia nauplii, the seedlings are transferred to a water nursery pond of 10-15 m<2> for breeding, the seedlings are fed with artemia nauplii, blood worms, cyclops or opossum shrimps, and when juvenile glossogobius giuris grows to 15-25 mm in total length, the conventional fingerling breeding can be performed.
Description
Technical field
Propagation technique, especially the tongue Acenthogobius flavimanus indoors artificial seedling growing process of this invention She Ji Acenthogobius flavimanus section fish.
Background technology
She Acenthogobius flavimanus
(Glossogobius giuris)on taxonomy, be under the jurisdiction of Perciformes (Perciformes) goby suborder (Gobioidei) Gobiidae (Gobiidae) goby belong to (
glossogobius), be the middle-size and small-size fish of a kind of water warm coastal waters bottom, move in tidal-flat area, degree of saltiness pool, river mouth, also can enter fresh water; She Acenthogobius flavimanus originates in the South Sea and the East Sea, and fish body circle is long, anterior cylindrical, and caudal peduncle is long flat-sided, and lip is thick, tongue front end bifurcated; She Acenthogobius flavimanus ingest small fish shrimp and organic debris etc.; Approximately 2 years maturations of tongue Acenthogobius flavimanus, body length is at 15-20 centimetre; Be the spring and summer 4-8 month breeding period, milter husky mud under stone or more flat substrate cave of nesting, and raun enters cave and lays eggs.
She Acenthogobius flavimanus fine and tender taste, delicious flavour, nutritive value is good, and economic worth is high, is deeply subject to liking of littoral fisherman, but because its individuality is little, quantity is few, does not come into one's own for a long time, also very limited to its research both at home and abroad, rarely seenly to the simple description of its morphological feature and biological property with to the muscle nutrition composition of tongue Acenthogobius flavimanus, analyze etc., and have no the relevant artificial propagation techniques of fish therewith; Tongue Acenthogobius flavimanus also occupies very important status in the food of Acipenser sinensis juvenile fish forms, also can be as the fancy fishes of the Shui nationality; In recent years, owing to being subject to the impact of environmental pollution, cause She Acenthogobius flavimanus species seriously to decay; Need to carry out artificial breeding and cultivation, be enhancement releasing, repairs natural resources and offer help.
Summary of the invention
The object of this invention is to provide a kind of She Acenthogobius flavimanus indoors artificial seedling-cultivating method.
Technical scheme of the present invention You indoor reinforced cultivating, the artificial repening of She Acenthogobius flavimanus parent population hasten parturition, artificial incubation and seed rearing step form, it is characterized in that adopting from natural sea-water waters and catch wild She Acenthogobius flavimanus, whose body weight is at 30-50 gram, adopt indoor cement pit to cultivate, at the bottom of pond, lay yellow sand and cavernous things concealed, yellow sand is laid area and is accounted for 2/3rds of pond floor space, thickness is 10-15cm, it is 8-10cm that cavernous things concealed adopts diameter, length is the plastics immersed tube of 25-30cm, and it is 3-5/m that pipe lays density
2; In pond, the depth of water is 1.1-1.2 rice, and salinity is 10-15, and breeding density is 5-10 tail/m
2; The small fish shrimp of throwing something and feeding every day is main fresh food, and day feeding volume accounts for the 2-10% of fish body weight; Collocation eel feed, a day feeding volume is the 1-2% of fish body weight; Carry out overwintering culture winter, and water temperature is controlled at 10-15 ℃; Every day soil pick-up once, in the time of blowdown, check the situation of ingesting of tongue Acenthogobius flavimanus, adjust the bait feeding amount of tongue Acenthogobius flavimanus; Change weekly water once, monthly fall pond once, quantity of exchanged water is 70-80%, while changing water, notes temperature and the salinity of fresh seawater, controls the temperature difference≤± 0.5 ℃, salinity is poor≤and ± 0.5; When ocean temperature reaches more than 20 ℃, select that physically fit vigor is good, body surface smooth is without scar, the good She of gonad development Acenthogobius flavimanus, the gonad development of routine observation parent population, treats that water temperature reaches 24-25 ℃ and can carry out artificial repening by common mode and hasten parturition; Spawning pond Nei She Acenthogobius flavimanus parent population is put male and female in a suitable place to breed than being 1:1.5-2, and density is 5-10 tail/m
2; At the bottom of pond, lay She Acenthogobius flavimanus laying nest, laying nest liner 40-60 object net sheet, it is 5-6/m that laying nest is placed quantity
2; Check laying nest every day, observe the situation of laying eggs of She Acenthogobius flavimanus, once lay eggs, take out in time the net sheet that is adhering to fertilized egg, net sheet is evened up and is hung vertically in 10-15m
2hatchery in; Process the early stage of hatching seawater, in cistern, with the sterilization of 50ppm bleaching powder, sterilizes after 10 days, and fully aeration can be used after removing chlorine residue; Fertilized egg is finger-like type, and epitheca film length is 8-10 millimeter, and the thick rear end of front end is thin, and embryo is sheath Inner Front End outside, and rear end sticks on net sheet, and fertilized egg is shaken with water; Under the condition of water temperature 25-28 ℃, hatching 5-7 days can membrane; She Acenthogobius flavimanus is grown seedlings and is adopted rear the fry in fertile pond method, and seawater salinity is controlled at 10-15, and temperature is at 25-28 ℃; Within after membrane the 3rd day, start the yolk of throwing something and feeding, every day, 8:00,12:00,16:00 and 20:00 respectively threw something and fed once, rubbed with the hands brokenly while throwing something and feeding with 100-150 object bolting silk, now rubbed existing use with the hands, evenly splashed, and continued 5-7 days, the 1ppm that feeding volume is water body; The seawater free wheel worm of simultaneously throwing something and feeding, density rests in 15-20/ml in nursery pond, continues 20-25 days; That throws something and feeds seawater free wheel worm starts hold concurrently to throw artemia nauplii on the 15th day, and density is 5-10/ml, continues 20-25 days; Throw something and feed the 30th day bait such as red worm, cyclops or oppossum shrimp that start to throw something and feed of seawater free wheel worm; Every day soil pick-up once, change water once, quantity of exchanged water 50-70%, temperature difference of seawater≤± 0.5 ℃, salinity is poor≤± 0.5; Through the cultivation of 40-45 days, from yolk seedling, growing into gradually length was 15-25mm She Acenthogobius flavimanus juvenile fish.
Outstanding feature of the present invention is in the indoor reinforced cultivating pond of: ⑴ She Acenthogobius flavimanus parent population, to lay yellow sand and cavernose things concealed, and the She Acenthogobius flavimanus of Weiing is created the habitat of life growth; (2) by using hormone, improve the synchronism of parent population gonad development, concentrated the laying eggs of induction She Acenthogobius flavimanus, realizes She Acenthogobius flavimanus indoor raising of seedling production scale; (3) special-purpose laying nest is set, and liner 40-60 object plastic deeping, builds a Ge She Acenthogobius flavimanus egg-laying environment, can effectively collect fertilized egg, is convenient to fertilized egg and concentrates hatching; (4) adopt the net sheet vertical hanging that is adhering to fertilized egg, increase the water body exchange around of net sheet, improve incubation rate, also for concentrating a hatching minute distribution seedling to provide convenience, (check embryonic development situation on different masses net sheet, the fertilized egg of similar time membrane, according to cloth seedling density requirements, is suspended on to wait membrane in same nursery pond); (5) adopt rear the fry in fertile pond, the overlapping principle of throwing something and feeding of the agreeable to the taste bait of different times, it is 7-10 days that transit adapts to the overlapping phase, the survival rate that is conducive to grow seedlings.Use the technology of the present invention means, obtain first artificial breeding She Acenthogobius flavimanus juvenile fish 7.3 ten thousand tails, successfully realize She Acenthogobius flavimanus indoors artificial and grow seedlings.
Embodiment
Embodiment 1: a kind of concrete implementation step of She Acenthogobius flavimanus indoors artificial seedling-cultivating method is as follows:
The indoor reinforced cultivating of She Acenthogobius flavimanus parent population: adopt from natural sea-water waters and catch wild She Acenthogobius flavimanus, whose body weight 30-50 gram; Wei She Acenthogobius flavimanus, create the habitat of life growth, adopt indoor cement pit to cultivate, at the bottom of pond, lay yellow sand and cavernous things concealed, yellow sand is laid area and is accounted for 2/3rds of pond floor space, thickness is 10-15cm, it is 8-10cm that cavernous things concealed adopts diameter, the plastics immersed tube that length is 25-30cm, and it is 3-5/m that pipe lays density
2, in pond, the depth of water is 1.1-1.2 rice, and salinity is 10-15, and breeding density is 5-10 tail/m
2; The small fish shrimp of throwing something and feeding every day is main fresh food, and day feeding volume accounts for the 2-10% of fish body weight; Collocation eel feed, a day feeding volume is the 1-2% of fish body weight; Carry out overwintering culture winter, and water temperature is controlled at 10-15 ℃, guarantees that She Acenthogobius flavimanus normally ingests, and meets the She Acenthogobius flavimanus required energy of living; Every day soil pick-up once, play the situation of ingesting that blowdown effect can check again She Acenthogobius flavimanus simultaneously, adjust the bait feeding amount of She Acenthogobius flavimanus; Change weekly water once, monthly fall pond once, quantity of exchanged water is 70-80%, while changing water, to note temperature and the salinity of fresh seawater, control the temperature difference≤± 0.5 ℃, salinity is poor≤and ± 0.5, reduce and cause the impact of the sudden change of temperature and salinity on parent fish rearing because changing water, through 8-10 month, cultivate and can become standby parent;
Artificial repening is hastened parturition: when ocean temperature reaches more than 20 ℃, select that physically fit vigor is good, body surface smooth is without scar, the good She of gonad development Acenthogobius flavimanus, the gonad development of routine observation parent population, treats that water temperature reaches 24-25 ℃ and can carry out artificial repening and hasten parturition, with short Luteinizing hormone releasing hormone A
2(LHRH-A
2) and human chorionic gonadtropin (HCG) carry out induced breeding, its objective is the synchronism that improves male and female parent population gonad development, induction parent population is concentrated and lays eggs, and is beneficial to large-scale production; Hormone adopts the two-stage method of accelerating the ripening, and the first order is used short Luteinizing hormone releasing hormone A
2(LHRH-A
2) and the mixed liquor of human chorionic gonadtropin (HCG), the short Luteinizing hormone releasing hormone of raun and human chorionic gonadtropin dosage are respectively the 1-2ug/kg(LHRH-A of She Acenthogobius flavimanus parent population body weight
2) and 500-1000IU/kg (HCG), short Luteinizing hormone releasing hormone A is used at interval after 48 hours
2(LHRH-A
2) dosage is parent population body weight 5-10ug/kg, general effector phase, can be extruded ovum after 24-36 hour; It is raun 1/2 that milter is used the dosage of hormone; Spawning pond Nei She Acenthogobius flavimanus parent population is put male and female in a suitable place to breed than being 1:1.5-2, and density is 5-10 tail/m
2; At the bottom of pond, lay She Acenthogobius flavimanus tubulose laying nest, laying nest liner 40-60 object net sheet, it is 5-6/m that laying nest is placed quantity
2; Check laying nest every day, observe the situation of laying eggs of She Acenthogobius flavimanus, once lay eggs, take out in time the net sheet that is adhering to fertilized egg; She Acenthogobius flavimanus fertilized egg sticks on net sheet more uniformly, and net sheet is evened up and is hung vertically in 10-15m
2hatchery in;
Artificial incubation: process the early stage of hatching seawater with the sterilization of 50ppm bleaching powder, is sterilized after 10 days in cistern, and fully aeration can be used after removing chlorine residue; Fertilized egg is finger-like type, and epitheca film length is 8-10 millimeter, and the thick rear end of front end is thin, and embryo is sheath Inner Front End outside, and rear end sticks to ， She Acenthogobius flavimanus fertilized egg on net sheet and sticks to more uniformly on net sheet, and net sheet is evened up and is hung vertically in 10-15m
2hatchery in, be convenient to increase the water body exchange around of net sheet, net sheet is rolled and is tended to fertilized egg to be wrapped in the inside, easily causes that fertilized egg sticks together and mouldy, causes fertilized egg embryonic development difficulty and stops; Fertilized egg is shaken with water; Under the condition of water temperature 25-28 ℃, generally hatching 5-7 days can membrane;
Seed rearing: She Acenthogobius flavimanus is grown seedlings and is adopted rear the fry in fertile pond method, and seawater salinity is controlled at 10-15, and temperature is at 25-28 ℃; Within after membrane the 3rd day, start the yolk of throwing something and feeding, every day, 8:00,12:00,16:00 and 20:00 respectively threw something and fed once, rubbed with the hands brokenly while throwing something and feeding with 100-150 object bolting silk, now rubbed existing use with the hands, evenly splashed, and continued 5-7 days, and feeding volume is water body 1ppm; The seawater free wheel worm of simultaneously throwing something and feeding, density rests in 15-20/ml in nursery pond, continues 20-25 days; That throws something and feeds seawater free wheel worm starts hold concurrently to throw artemia nauplii on the 15th day, and density is 5-10/ml, continues 20-25 days; Throw something and feed the 30th day bait such as red worm, cyclops or oppossum shrimp that start to throw something and feed of seawater free wheel worm; Every day soil pick-up once, change water once, quantity of exchanged water 50-70%, temperature difference of seawater≤± 0.5 ℃, salinity is poor≤± 0.5; The cultivation of 40-45 days of general process, from yolk seedling, growing into gradually length is 15-25mm She Acenthogobius flavimanus juvenile fish.
Embodiment 2: according to the mode described in embodiment 1, adopt catch wild She Acenthogobius flavimanus selective body length at the individuality of 10-15 centimetre from natural sea-water waters; Between the incubation period, take out net sheet and observe embryonic development every day, measures temperature, salinity, changes every other day water 50%, uninterrupted micro-inflation continuously, and gas exhausting stone density is 0.5-1/m
2; Nursery pond specification is 115 * 115 * 100cm, the cement pit of bottom taper, seedling water 1000kg; Each pond hangs a fertilized egg net sheet, injects a certain amount of algae water after membrane.
Claims (4)
1. a tongue Acenthogobius flavimanus indoors artificial seedling-cultivating method; indoor reinforced cultivating, the artificial repening by tongue Acenthogobius flavimanus parent population hastened parturition, artificial incubation and seed rearing step form, it is characterized in that adopting from natural sea-water waters and catch wild She Acenthogobius flavimanus, whose body weight is at 30-50 gram, adopt indoor cement pit to cultivate, at the bottom of pond, lay yellow sand and cavernous things concealed, yellow sand is laid area and is accounted for 2/3rds of pond floor space, thickness is 10-15cm, it is 8-10cm that cavernous things concealed adopts diameter, length is the plastics immersed tube of 25-30cm, and it is 3-5/m that pipe lays density
2; In pond, the depth of water is 1.1-1.2 rice, and salinity is 10-15, and breeding density is 5-10 tail/m
2; The small fish shrimp of throwing something and feeding every day is main fresh food, and day feeding volume accounts for the 2-10% of fish body weight; Collocation eel feed, a day feeding volume is the 1-2% of fish body weight; Carry out overwintering culture winter, and water temperature is controlled at 10-15 ℃; Every day soil pick-up once, in the time of blowdown, check the situation of ingesting of tongue Acenthogobius flavimanus, adjust the bait feeding amount of tongue Acenthogobius flavimanus; Change weekly water once, monthly fall pond once, quantity of exchanged water is 70-80%, while changing water, notes temperature and the salinity of fresh seawater, controls the temperature difference≤± 0.5 ℃, salinity is poor≤and ± 0.5; When ocean temperature reaches more than 20 ℃, select that physically fit vigor is good, body surface smooth is without scar, the good She of gonad development Acenthogobius flavimanus, the gonad development of routine observation parent population, treats that water temperature reaches 24-25 ℃ and by common mode, carries out artificial repening and hasten parturition; Spawning pond Nei She Acenthogobius flavimanus parent population is put male and female in a suitable place to breed than being 1:1.5-2, and density is 5-10 tail/m
2; At the bottom of pond, lay She Acenthogobius flavimanus laying nest, laying nest liner 40-60 object net sheet, it is 5-6/m that laying nest is placed quantity
2; Check laying nest every day, observe the situation of laying eggs of She Acenthogobius flavimanus, once lay eggs, take out in time the net sheet that is adhering to fertilized egg, net sheet is evened up and is hung vertically in 10-15m
2hatchery in; Process the early stage of hatching seawater, in cistern, with the sterilization of 50ppm bleaching powder, sterilizes after 10 days, and fully aeration can be used after removing chlorine residue; Fertilized egg is finger-like type, and epitheca film length is 8-10 millimeter, and the thick rear end of front end is thin, and embryo is sheath Inner Front End outside, and rear end sticks on net sheet, and fertilized egg is shaken with water; Under the condition of water temperature 25-28 ℃, hatching 5-7 days can membrane; She Acenthogobius flavimanus is grown seedlings and is adopted rear the fry in fertile pond method, and seawater salinity is controlled at 10-15, and temperature is at 25-28 ℃; Within after membrane the 3rd day, start the yolk of throwing something and feeding, every day, 8:00,12:00,16:00 and 20:00 respectively threw something and fed once, rubbed with the hands brokenly while throwing something and feeding with 100-150 object bolting silk, now rubbed existing use with the hands, evenly splashed, and continued 5-7 days, the 1ppm that feeding volume is water body; The seawater free wheel worm of simultaneously throwing something and feeding, density rests in 15-20/ml in nursery pond, continues 20-25 days; That throws something and feeds seawater free wheel worm starts hold concurrently to throw artemia nauplii on the 15th day, and density is 5-10/ml, continues 20-25 days; Throw something and feed the 30th day bait such as red worm, cyclops or oppossum shrimp that start to throw something and feed of seawater free wheel worm; Every day soil pick-up once, change water once, quantity of exchanged water 50-70%, temperature difference of seawater≤± 0.5 ℃, salinity is poor≤± 0.5; Through the cultivation of 40-45 days, from yolk seedling, growing into gradually length was 15-25mm She Acenthogobius flavimanus juvenile fish.
2. a kind of She Acenthogobius flavimanus indoors artificial seedling-cultivating method according to claim 1, is characterized in that natural sea-water waters is adopted to catch wild She Acenthogobius flavimanus selective body length at the individuality of 10-15 centimetre.
3. a kind of She Acenthogobius flavimanus indoors artificial seedling-cultivating method according to claim 1, is characterized in that between the incubation period that take out net sheet observation embryonic development every day, measures temperature, salinity, changes every other day water 50%, uninterrupted micro-inflation continuously, and gas exhausting stone density is 0.5-1/m
2.
4. a kind of She Acenthogobius flavimanus indoors artificial seedling-cultivating method according to claim 1, is characterized in that nursery pond specification is 115 * 115 * 100cm, the cement pit of bottom taper, seedling water 1000kg; Each pond hangs a fertilized egg net sheet, injects a certain amount of algae water after membrane.
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| CN105994100A (en) * | 2016-06-15 | 2016-10-12 | 浙江省海洋水产研究所 | Marine fish fry breeding device and method |
| CN106688970A (en) * | 2016-11-15 | 2017-05-24 | 中国水产科学研究院东海水产研究所 | Method for artificially breeding fingerlings of siganus vulpinus in indoor environments |
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| US20220284612A1 (en) * | 2020-10-23 | 2022-09-08 | X Development Llc | Visual detection of haloclines |
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| CN105994100A (en) * | 2016-06-15 | 2016-10-12 | 浙江省海洋水产研究所 | Marine fish fry breeding device and method |
| CN106688970A (en) * | 2016-11-15 | 2017-05-24 | 中国水产科学研究院东海水产研究所 | Method for artificially breeding fingerlings of siganus vulpinus in indoor environments |
| CN107027663A (en) * | 2017-05-03 | 2017-08-11 | 上海市水产研究所 | A kind of indoor cement pit cultural method of the cabezon fingerling of artificial breeding |
| CN107211923A (en) * | 2017-05-03 | 2017-09-29 | 上海市水产研究所 | A kind of Synechogobius ommaturus ecological breeding method |
| CN107027663B (en) * | 2017-05-03 | 2020-04-03 | 上海市水产研究所 | Indoor cement pond culture method for artificially-bred sebastes marmoratus fingerlings |
| CN112042567A (en) * | 2020-07-29 | 2020-12-08 | 中国水产科学研究院南海水产研究所 | All-season cultivation method for parent fish of marine fishes |
| US20220284612A1 (en) * | 2020-10-23 | 2022-09-08 | X Development Llc | Visual detection of haloclines |
| CN113317241A (en) * | 2021-06-09 | 2021-08-31 | 中国水产科学研究院东海水产研究所 | Method for gonadotropic development and artificial induced spawning of parent fish of takifugu hexamaculatus |
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