CN102428884A - Large-scale total artificial breeding method of Sepiella maindroni - Google Patents

Large-scale total artificial breeding method of Sepiella maindroni Download PDF

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Publication number
CN102428884A
CN102428884A CN2011102761747A CN201110276174A CN102428884A CN 102428884 A CN102428884 A CN 102428884A CN 2011102761747 A CN2011102761747 A CN 2011102761747A CN 201110276174 A CN201110276174 A CN 201110276174A CN 102428884 A CN102428884 A CN 102428884A
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pond
breeding
water
density
bait
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周瑞发
谢友亮
刘振勇
阮经盛
彭立成
夏道演
林钦爱
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NINGDE NANHAI AQUATIC PRODUCTS TECHNOLOGY Co Ltd
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NINGDE NANHAI AQUATIC PRODUCTS TECHNOLOGY Co Ltd
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Priority to CN2011102761747A priority Critical patent/CN102428884A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

A large-scale total artificial breeding method of Sepiella maindroni is provided, comprising three steps of parent breeding, artificial propagation and seedling breeding. The cultivated fish or wild fish with excellent biological characteristics is selected as parent; the selected fish is bred in an indoor cement pond or a pond or a seawater net cage; the bred cuttlefish is put to a spawning nest in a spawning pond to spawn; collected fertilized ova are transferred to a hatching pond after being sterilized; Chlorella is put to the hatching pond to manage patching; after larva is hatched, the larva is bred in the breeding pond; the seedling is artificially bred in large scale by controlling breeding environment and breeding density, selecting larva breeding bait series and feeding method. The breeding method is suitable for a large-scale total artificial breeding method of Sepiella maindroni, and has the advantages of high batching rate, high survival rate, less disease, high seedling quantity, easy breeding and fast growth speed and so on; the breeding method is easy to operate and has high success rate, so the method is suitable for being popularized in large scale.

Description

A kind of Sepiella maindroni scale artificial seedling rearing method
Technical field
The invention belongs to cuttlefish seedling-cultivating method field, more specifically relate to a kind of Sepiella maindroni scale artificial seedling rearing method.
Background technology
Sepiella maindroni (being commonly called as inkfish) once was that China's sea fishery four big masters catch one of object, was the fishery resources of a kind of preciousness of China, since overfishing, the very big destruction of having caused resource, natural resources is obviously decayed, and output sharply descends, and is endangered.For recovering this precious species resource, Ningde City South Sea Aqua Sciences Inc. took the lead in carrying out Sepiella maindroni in Fujian Province in 2006 and grows seedlings, and successfully cultivated seed and cultured.Carried out technological innovation in 2007,2008, and obtained major progress, broken through the technology that large-scale artificial is grown seedlings " bottleneck ", reached the level of growing seedlings in enormous quantities, the amount of growing seedlings is the hat in the whole nation, has formed a kind of Sepiella maindroni scale artificial seedling rearing technology.The seed of cultivating provides ground such as Ningde, Xiapu, Fuding, Liaanjiang county, Luoyuan to carry out the breed of pond, cement pit and seawater cage isotype.This achievement; Have a series of Sepiella maindronis the grow seedlings technology and the achievement of multinomial leading domestic level; " Sepiella maindroni scale artificial seedling rearing technology " obtains Ningde City scientific and technological progress second prize; To protection and recovery marine site marine organisms diversity, advance the steady healthy sustainable development of sea fishery significant.
Summary of the invention
The objective of the invention is provides the scale artificial seedling rearing method to Sepiella maindroni; This seedling-cultivating method is applicable to the operation of growing seedlings of the large-scale artificial of Sepiella maindroni; It has that incubation rate height, survival rate are high, disease is few, the amount of growing seedlings height, be easy to culture, advantage such as growth is fast; And the operation easy to implement of this seedling-cultivating method, success rate height, suitable large-scale promotion.
A kind of Sepiella maindroni scale artificial seedling rearing method, it has following steps:
(1) parents culture: select good breed adult fish of biological character or wild adult fish as parent, in indoor cement pit or pond or seawater cage, cultivate, cultivated 1 month;
(2) artificial propagation: the laying nest that the cuttlefish parent after will cultivating is placed in the spawning pond is laid eggs; Collect fertilized egg sterilization back and move into the hatchery; In the hatchery, add chlorella and improve water quality, change water every day and carry out hatching management, treat that the young hatches the back and in nursery pond, cultivates;
(3) seed rearing: through control nurturing an environment, cultivation density, screening larval culture bait series and feeding method carry out the scale artificial seedling rearing.
The parent bodily form of selecting in the said parents culture is complete, stalwartness, body weight 100-150g.Female, male proportioning is 1:1.
The nurturing an environment of said parents culture is selected indoor cement pit or seawater cage, pond rearing; Depth of water 1.5-2m, condition of water quality are salinity 23-28, guarantee that water inlet and draining are smooth and easy; The water yield is sufficient, the water of nursery pond continues heating or recycle through boiler; Indoor Winter protection, summer ventilation and heat, through aerating machine oxygenation; The roof can draw back closed window or curtain, and is indoor through fluorescent lamp adjusting luminous intensity 50-100lux.
Said cuttlefish parent increases feeding volume 30-50% every day after parents culture is reach 1 month, and bait is good shrimp of high-quality freshness and small fish, promotes the parent gonad maturity, 50/m of parents culture density 3Bait is bait with fresh shrimp, clam worm, and daily ration, feeding quantity is 8% of a physique amount, throws something and feeds every day 2 times.The roof can draw back closed window or curtain, and is indoor through fluorescent lamp adjusting 50-100lux, keeps cultivating 18 ℃ of water temperatures.
Said artificial propagation may further comprise the steps:
1. lay eggs: suspension is with 18 in spawning pond #Iron wire is that frame, specification are that 30 * 50cm, mesh are that the nylon mesh sheet of 1 * 1cm is made the laying nest laying nest that forms, and is used for inkfish spawning; Treat can will net the sheet taking-up after laying nest is covered with fertilized egg, the sterilization back moves into the hatchery;
2. hatching: with 1m 3Cement pit as the hatchery, will net sheet and be suspended in the water body and hatch, hatching density is ten thousand/m of 5-8 3, hydrostatic is hatched through aerating machine oxygenation.Collect the young and move into 3.3 * 8.2 * 1.5 m 3Or 4 * 9 * 1.5m 3Nursery pond grow seedlings, perhaps directly fertilized egg is placed 3.3 * 8.2 * 1.5 m 3Or 4 * 9 * 1.5m 3Nursery pond in hatch hatching density 2~30,000 ovum/m 3, directly stay former pond after the young hatches and cultivate.
Said hatching water temperature is 22-26 ℃, and salinity range is 25.7-29.7; Keep DO at 5-10 mg/ml.
It cultivates 23 ℃-30 ℃ of water temperatures said seed rearing; Between the salinity range 23.1-33.0, pH value 8.0; Ammonia nitrogen is at 0.01-0.02mg/L; Control illuminance 1000-5000Lux.Keep dissolved oxygen 5-10 mg/ml.Just incubate larval culture density and be controlled at 1.5-2 ten thousand tails/m 2, the middle and later periods is reduced cultivation density gradually, and it is 2000-5000/m that the later stage is cultivated density 3
Cuttlefish cultivation bait series is in the said seed rearing: artemia nauplii → copepoda → work mysis → dead oppossum shrimp; The step of throwing something and feeding is: the artemia nauplii of can directly ingesting when cuttlefish just incubates young opening, and food density remains on 5/ml; Throw something and feed behind 3 ages in days copepoda and nauplius thereof; Behind the long 1.2cm of cuttlefish seed trunk, begin to attempt dead bait and tame and docile food, until most of young dead bait of ingesting.
The present invention compared with prior art, the operation easy to implement of this seedling-cultivating method, incubation rate is high, survival rate is high, disease is few, the amount of growing seedlings height, be easy to culture, advantage, suitable large-scale promotion such as growth is fast.
Embodiment
Below in conjunction with embodiment the present invention is further described.
Embodiment 1
A kind of Sepiella maindroni scale artificial seedling rearing method, practical implementation step of the present invention is:
(1) parents culture: select the good breed adult fish of biological character as parent, in indoor cement pit, cultivated 1 month;
(2) artificial propagation: the laying nest that the cuttlefish parent after will cultivating is placed in the spawning pond is laid eggs; Collect fertilized egg sterilization back and move into the hatchery; In the hatchery, add chlorella and improve water quality, change water every day and carry out hatching management, treat that the young hatches the back and in nursery pond, cultivates;
(3) seed rearing: through control nurturing an environment, cultivation density, screening larval culture bait series and feeding method carry out the scale artificial seedling rearing.
The parent bodily form of selecting in the said parents culture is complete, stalwartness, body weight 100g.Female, male proportioning is 1:1.
The nurturing an environment of said parents culture is selected indoor cement pit or seawater cage, pond rearing; The depth of water 1.5 m, condition of water quality are salinity 23, guarantee that water inlet and draining are smooth and easy; The water yield is sufficient, the water of nursery pond continues heating or recycle through boiler; Indoor Winter protection, summer ventilation and heat, through aerating machine oxygenation; The roof can draw back closed window or curtain, and is indoor through fluorescent lamp adjusting luminous intensity 50lux.
Said cuttlefish parent increases feeding volume 30% every day after parents culture is reach 1 month, and bait is good shrimp of high-quality freshness and small fish, promotes the parent gonad maturity, 50/m of parents culture density 3Bait is bait with fresh shrimp, clam worm, and daily ration, feeding quantity is 8% of a physique amount, throws something and feeds every day 2 times.The roof can draw back closed window or curtain, and is indoor through fluorescent lamp adjusting 50lux, keeps cultivating 18 ℃ of water temperatures.
Said artificial propagation may further comprise the steps:
1. lay eggs: suspension is with 18 in spawning pond #Iron wire is that frame, specification are that 30 * 50cm, mesh are that the nylon mesh sheet of 1 * 1cm is made the laying nest laying nest that forms, and is used for inkfish spawning; Treat can will net the sheet taking-up after laying nest is covered with fertilized egg, the sterilization back moves into the hatchery;
2. hatching: with 1m 3Cement pit as the hatchery, will net sheet and be suspended in the water body and hatch, hatching density is 50,000/m 3, hydrostatic is hatched through aerating machine oxygenation.Collect the young and move into 3.3 * 8.2 * 1.5 m 3Nursery pond grow seedlings
Said hatching water temperature is 22 ℃, and salinity is 25.7; Keep DO at 5mg/ml.
It cultivates 23 ℃ of water temperatures said seed rearing, and salinity is 23.1, pH value 8.0, and ammonia nitrogen is 0.01mg/L; Control illuminance 1000Lux.Keep dissolved oxygen 5 mg/ml.Just incubate larval culture density and be controlled at 1.5 ten thousand tails/m 2, the middle and later periods is reduced cultivation density gradually, and it is 2000/m that the later stage is cultivated density 3
Cuttlefish cultivation bait series is in the said seed rearing: artemia nauplii → copepoda → work mysis → dead oppossum shrimp; The step of throwing something and feeding is: the artemia nauplii of can directly ingesting when cuttlefish just incubates young opening, and food density remains on 5/ml; Throw something and feed behind 3 ages in days copepoda and nauplius thereof; Behind the long 1.2cm of cuttlefish seed trunk, begin to attempt dead bait and tame and docile food, until most of young dead bait of ingesting.
Embodiment 2
A kind of Sepiella maindroni scale artificial seedling rearing method, its invention practical implementation step is:
(1) parents culture: select the good wild adult fish of biological character as parent, in indoor cement pit, cultivated 1 month;
(2) artificial propagation: the laying nest that the cuttlefish parent after will cultivating is placed in the spawning pond is laid eggs; Collect fertilized egg sterilization back and move into the hatchery; In the hatchery, add chlorella and improve water quality, change water every day and carry out hatching management, treat that the young hatches the back and in nursery pond, cultivates;
(3) seed rearing: through control nurturing an environment, cultivation density, screening larval culture bait series and feeding method carry out the scale artificial seedling rearing.
The parent bodily form of selecting in the said parents culture is complete, stalwartness, body weight 150g.Female, male proportioning is 1:1.
The nurturing an environment of said parents culture is selected indoor cement pit or seawater cage, pond rearing; The depth of water 2 m, condition of water quality are salinity 28, guarantee that water inlet and draining are smooth and easy; The water yield is sufficient, the water of nursery pond continues heating or recycle through boiler; Indoor Winter protection, summer ventilation and heat, through aerating machine oxygenation; The roof can draw back closed window or curtain, and is indoor through fluorescent lamp adjusting luminous intensity 100lux.
Said cuttlefish parent increases feeding volume 50% every day after parents culture is reach 1 month, and bait is good shrimp of high-quality freshness and small fish, promotes the parent gonad maturity, 50/m of parents culture density 3Bait is bait with fresh shrimp, clam worm, and daily ration, feeding quantity is 8% of a physique amount, throws something and feeds every day 2 times.The roof can draw back closed window or curtain, and is indoor through fluorescent lamp adjusting 100lux, keeps cultivating 18 ℃ of water temperatures.
Said artificial propagation may further comprise the steps:
1. lay eggs: suspension is with 18 in spawning pond #Iron wire is that frame, specification are that 30 * 50cm, mesh are that the nylon mesh sheet of 1 * 1cm is made the laying nest laying nest that forms, and is used for inkfish spawning; Treat can will net the sheet taking-up after laying nest is covered with fertilized egg, the sterilization back moves into the hatchery;
2. hatching: with 1m 3Cement pit as the hatchery, will net sheet and be suspended in the water body and hatch, hatching density is 80,000/m 3, hydrostatic is hatched through aerating machine oxygenation.Collect the young and move into 3.3 * 8.2 * 1.5 m 3Nursery pond is grown seedlings, and perhaps directly fertilized egg is placed 3.3 * 8.2 * 1.5 m 3Nursery pond in hatch 30,000 ovum/m of hatching density 3, directly stay former pond after the young hatches and cultivate.
Said hatching water temperature is 26 ℃, and salinity is 29.7; Keep DO at 10 mg/ml.
It cultivates 30 ℃ of water temperatures said seed rearing, and salinity is 33.0, pH value 8.0, and ammonia nitrogen is 0.02mg/L; Control illuminance 5000Lux.Keep dissolved oxygen 10 mg/ml.Just incubate larval culture density and be controlled at 20,000 tails/m 2, the middle and later periods is reduced cultivation density gradually, and it is 5000/m that the later stage is cultivated density 3
Cuttlefish cultivation bait series is in the said seed rearing: artemia nauplii → copepoda → work mysis → dead oppossum shrimp; The step of throwing something and feeding is: the artemia nauplii of can directly ingesting when cuttlefish just incubates young opening, and food density remains on 5/ml; Throw something and feed behind 3 ages in days copepoda and nauplius thereof; Behind the long 1.2cm of cuttlefish seed trunk, begin to attempt dead bait and tame and docile food, until most of young dead bait of ingesting.
Embodiment 3
A kind of Sepiella maindroni scale artificial seedling rearing method, its invention practical implementation step is:
(1) parents culture: select good breed adult fish of biological character or wild adult fish as parent, in indoor cement pit, cultivated 1 month;
(2) artificial propagation: the laying nest that the cuttlefish parent after will cultivating is placed in the spawning pond is laid eggs; Collect fertilized egg sterilization back and move into the hatchery; In the hatchery, add chlorella and improve water quality, change water every day and carry out hatching management, treat that the young hatches the back and in nursery pond, cultivates;
(3) seed rearing: through control nurturing an environment, cultivation density, screening larval culture bait series and feeding method carry out the scale artificial seedling rearing.
The parent bodily form of selecting in the said parents culture is complete, stalwartness, body weight 120g.Female, male proportioning is 1:1.
The nurturing an environment of said parents culture is selected indoor cement pit or seawater cage, pond rearing; Depth of water 1.7m, condition of water quality are salinity 26, guarantee that water inlet and draining are smooth and easy; The water yield is sufficient, the water of nursery pond continues heating or recycle through boiler; Indoor Winter protection, summer ventilation and heat, through aerating machine oxygenation; The roof can draw back closed window or curtain, and is indoor through fluorescent lamp adjusting luminous intensity 75lux.
Said cuttlefish parent increases feeding volume 40% every day after parents culture is reach 1 month, and bait is good shrimp of high-quality freshness and small fish, promotes the parent gonad maturity, 50/m of parents culture density 3Bait is bait with fresh shrimp, clam worm, and daily ration, feeding quantity is 8% of a physique amount, throws something and feeds every day 2 times.The roof can draw back closed window or curtain, and is indoor through fluorescent lamp adjusting 75lux, keeps cultivating 18 ℃ of water temperatures.
Said artificial propagation may further comprise the steps:
1. lay eggs: suspension is with 18 in spawning pond #Iron wire is that frame, specification are that 30 * 50cm, mesh are that the nylon mesh sheet of 1 * 1cm is made the laying nest laying nest that forms, and is used for inkfish spawning; Treat can will net the sheet taking-up after laying nest is covered with fertilized egg, the sterilization back moves into the hatchery;
2. hatching: with 1m 3Cement pit as the hatchery, will net sheet and be suspended in the water body and hatch, hatching density is 60,000/m 3, hydrostatic is hatched through aerating machine oxygenation.Collect the young and move into 4 * 9 * 1.5m 3Nursery pond grow seedlings, perhaps directly fertilized egg is placed 4 * 9 * 1.5m 3Nursery pond in hatch 2.5 ten thousand ovum/m of hatching density 3, directly stay former pond after the young hatches and cultivate.
Said hatching water temperature is 25 ℃, and salinity is 27; Keep DO at 8mg/ml.
It cultivates 26 ℃ of water temperatures said seed rearing, salinity 27.0, and pH value 8.0, ammonia nitrogen is 0.015mg/L; Control illuminance 3000Lux.Keep dissolved oxygen 8mg/ml.Just incubate larval culture density and be controlled at 1.7 ten thousand tails/m 2, the middle and later periods is reduced cultivation density gradually, and it is 3500/m that the later stage is cultivated density 3
Cuttlefish cultivation bait series is in the said seed rearing: artemia nauplii → copepoda → work mysis → dead oppossum shrimp; The step of throwing something and feeding is: the artemia nauplii of can directly ingesting when cuttlefish just incubates young opening, and food density remains on 5/ml; Throw something and feed behind 3 ages in days copepoda and nauplius thereof; Behind the long 1.2cm of cuttlefish seed trunk, begin to attempt dead bait and tame and docile food, until most of young dead bait of ingesting.
The above embodiment is merely preferred embodiment of the present invention, and all equalizations of doing according to claim of the present invention change and modify, and all should belong to covering scope of the present invention.

Claims (8)

1. Sepiella maindroni scale artificial seedling rearing method is characterized in that having following steps:
(1) parents culture: select good breed adult fish of biological character or wild adult fish as parent, in indoor cement pit or pond or seawater cage, cultivate, cultivated 1 month;
(2) artificial propagation: the laying nest that the cuttlefish parent after will cultivating is placed in the spawning pond is laid eggs; Collect fertilized egg sterilization back and move into the hatchery; In the hatchery, add chlorella and improve water quality, change water every day and carry out hatching management, treat that the young hatches the back and in nursery pond, cultivates;
(3) seed rearing: through control nurturing an environment, cultivation density, screening larval culture bait series and feeding method carry out the scale artificial seedling rearing.
2. a kind of Sepiella maindroni scale artificial seedling rearing method according to claim 1, it is characterized in that: the parent bodily form of selecting in the said parents culture is complete, stalwartness, body weight 100-150g; Female, male proportioning is 1:1.
3. a kind of Sepiella maindroni scale artificial seedling rearing method according to claim 1 is characterized in that: the nurturing an environment of said parents culture is selected indoor cement pit or seawater cage, pond rearing, depth of water 1.5-2m; Condition of water quality is salinity 23-28; Guarantee that water inlet and draining are smooth and easy, the water yield is sufficient, the water of nursery pond continues heating or recycle, indoor Winter protection through boiler; Summer ventilation and heat, through aerating machine oxygenation; The roof can draw back closed window or curtain, and is indoor through fluorescent lamp adjusting luminous intensity 50-100lux.
4. a kind of Sepiella maindroni scale artificial seedling rearing method according to claim 1; It is characterized in that: parents culture after full 1 month every day increase feeding volume 30-50%; Bait is good shrimp of high-quality freshness and small fish, promotes the parent gonad maturity, 50/m of parents culture density 3Bait is bait with fresh shrimp, clam worm, and daily ration, feeding quantity is 8% of a physique amount, throws something and feeds every day 2 times; The roof can draw back closed window or curtain, and is indoor through fluorescent lamp adjusting 50-100lux, keeps cultivating 18 ℃ of water temperatures.
5. a kind of Sepiella maindroni scale artificial seedling rearing method according to claim 1, it is characterized in that: said artificial propagation may further comprise the steps:
1. lay eggs: suspension is with 18 in spawning pond #Iron wire is that frame, specification are that 30 * 50cm, mesh are that the nylon mesh sheet of 1 * 1cm is made the laying nest laying nest that forms, and is used for inkfish spawning; Treat can will net the sheet taking-up after laying nest is covered with fertilized egg, the sterilization back moves into the hatchery;
2. hatching: with 1m 3Cement pit as the hatchery, will net sheet and be suspended in the water body and hatch, hatching density is ten thousand/m of 5-8 3, hydrostatic through aerating machine oxygenation hatching, is collected the young and is moved into 3.3 * 8.2 * 1.5 m 3Or 4 * 9 * 1.5m 3Nursery pond grow seedlings, perhaps directly fertilized egg is placed 3.3 * 8.2 * 1.5 m 3Or 4 * 9 * 1.5m 3Nursery pond in hatch ten thousand ovum/m of hatching density 2-3 3, directly stay former pond after the young hatches and cultivate.
6. a kind of Sepiella maindroni scale artificial seedling rearing method according to claim 5, it is characterized in that: said hatching water temperature is 22-26 ℃, salinity range is 25.7-29.7; Keep DO at 5-10mg/ml.
7. a kind of Sepiella maindroni scale artificial seedling rearing method according to claim 1 is characterized in that: it cultivates 23-30 ℃ of water temperature said seed rearing; Between the salinity range 23.1-33.0, pH value 8.0; Ammonia nitrogen is at 0.01-0.02mg/L; Control illuminance 1000-5000Lux; Keep dissolved oxygen 5-10 mg/L; Just incubate larval culture density and be controlled at 1.5-2 ten thousand tails/m 2, the middle and later periods is reduced cultivation density gradually, and it is 2000-5000/m that the later stage is cultivated density 3
8. a kind of Sepiella maindroni scale artificial seedling rearing method according to claim 1 is characterized in that: cuttlefish cultivation bait series is in the said seed rearing: artemia nauplii → copepoda → work mysis → dead oppossum shrimp; The step of throwing something and feeding is: the artemia nauplii of can directly ingesting when cuttlefish just incubates young opening, and food density remains on 5/ml; Throw something and feed behind 3 ages in days copepoda and nauplius thereof; Behind the long 1.2cm of cuttlefish seed trunk, begin to attempt dead bait and tame and docile food, until most of young dead bait of ingesting.
CN2011102761747A 2011-09-19 2011-09-19 Large-scale total artificial breeding method of Sepiella maindroni Pending CN102428884A (en)

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Cited By (16)

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CN102823529A (en) * 2012-09-17 2012-12-19 湛江师范学院 Sepia lycidas fry breeding method
CN102919180A (en) * 2012-11-21 2013-02-13 浙江海洋学院 Artificial breeding method of sepia esculenta
CN102919182A (en) * 2012-11-21 2013-02-13 浙江海洋学院 Propagation method of sepiella maindroni
CN102939924A (en) * 2012-11-13 2013-02-27 宁波大学 Method for artificially breeding sepia lycidas gray
CN103314900A (en) * 2013-06-17 2013-09-25 苏州市阳澄湖现代农业发展有限公司 Efficient breeding method for Sepiella maindroni de Rochebrune in pond
CN103392641A (en) * 2013-07-23 2013-11-20 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 Precision pond culture method for sepiella maindroni de Rochebrune
CN103493759A (en) * 2013-09-12 2014-01-08 宁德市南海水产科技有限公司 Grouper large scale total-artificial breeding method
CN103548732A (en) * 2013-10-30 2014-02-05 浙江海洋学院 Artificial offspring seed cultivating method for pharaoh cuttlefish
CN103636543A (en) * 2013-12-18 2014-03-19 上海市水产研究所 Indoor artificial breeding method of glossogobius giuris
CN103718992A (en) * 2013-11-27 2014-04-16 安徽省郎溪县水产管理站 Concentrated aeration breeding method of pseudorasbora parva fries
CN104957064A (en) * 2015-07-06 2015-10-07 合浦县庆丰农业科技开发有限公司 Method for culturing pharaoh cuttlefish fries
CN106900600A (en) * 2016-12-30 2017-06-30 浙江海洋大学 A kind of cultivation method for enhancing for improving Sepiella maindroni reproductive capacity
CN109362610A (en) * 2018-03-22 2019-02-22 中国水产科学研究院黄海水产研究所 Lai Shi intends cuttlefish offspring seed cultivation method
CN110402851A (en) * 2019-08-28 2019-11-05 浙江海洋大学 The method for culturing seedlings of tiger spot cuttlefish
CN112586411A (en) * 2020-12-31 2021-04-02 宁德市南海水产科技有限公司 Marine shellfish biological improved variety breeding method
CN113749021A (en) * 2021-10-12 2021-12-07 中国水产科学研究院黄海水产研究所 Method for cultivating sepia donovani fries

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CN103548732A (en) * 2013-10-30 2014-02-05 浙江海洋学院 Artificial offspring seed cultivating method for pharaoh cuttlefish
CN103548732B (en) * 2013-10-30 2016-01-13 浙江海洋学院 Tiger spot cuttlefish artificial seed breeding method
CN103718992A (en) * 2013-11-27 2014-04-16 安徽省郎溪县水产管理站 Concentrated aeration breeding method of pseudorasbora parva fries
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CN103636543A (en) * 2013-12-18 2014-03-19 上海市水产研究所 Indoor artificial breeding method of glossogobius giuris
CN103636543B (en) * 2013-12-18 2017-06-06 上海市水产研究所 A kind of She Acenthogobius flavimanus room heat source method
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CN106900600A (en) * 2016-12-30 2017-06-30 浙江海洋大学 A kind of cultivation method for enhancing for improving Sepiella maindroni reproductive capacity
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CN109362610A (en) * 2018-03-22 2019-02-22 中国水产科学研究院黄海水产研究所 Lai Shi intends cuttlefish offspring seed cultivation method
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CN110402851A (en) * 2019-08-28 2019-11-05 浙江海洋大学 The method for culturing seedlings of tiger spot cuttlefish
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Application publication date: 20120502