CN106376500A - Artificial fecundation releasing method for schizothorax chongi in Ya-lung River - Google Patents

Abstract

The invention discloses an artificial fecundation releasing method for schizothorax chongi in Ya-lung River. The method comprises seven steps of selecting parent fish, injecting oxytocic drug, performing artificial insemination, hatching germ cells, fostering fish larva, breeding juvenile fish and transporting and releasing. According to the method, the fecundation releasing of the schizothorax chongi in Ya-lung River can be effectively realized; the population of the schizothorax chongi in Ya-lung River can be recovered; the high valued schizothorax chongi in Ya-lung River can exert higher economic benefit, so that the method has ultrahigh economic and environment ecological benefits.

Description

Yalongjiang River thin squama schizothoracin artificial fecundation releases method

Technical field

Present invention relates particularly to a kind of thin squama schizothoracin artificial fecundation of Yalongjiang River releases method.

Background technology

Yalongjiang River is the maximum tributary of Upper Yangtze River Jinsha jiang River left bank, and it is important that thin squama schizothoracin is not only Yalong river valley Economic fish resource, is Upper Yangtze River Endemic fish simultaneously, is distributed mainly on the water such as Dadu River, Yalongjiang River, Jinsha jiang River and the Wujiang River System, has important function in terms of maintaining Yalongjiang River aquatic ecosystem balance.In recent years, fished for due to transition, water pollution, Hydraulic engineering and adopt the anthropic factors such as sand so that natural short hairs schizothoracin stock number degradation, have impact on society, warp in basin Ji and the benign cycle of ecological environment.

At present, many researcheres are also had to adopt the method for artificial fecundation to cultivate thin squama schizothoracin, the method is typically to utilize The wild sexual maturity adult fish fishing acquisition arranges more original incubator and carries out as parent fish, then on the Yalongjiang River nature water surface The artificial incubation of fish roe, is hatched using environmental conditions such as natural temperature, current；Although the method splits abdomen for increasing thin squama Fish fry quantity has certain help, but yet suffers from a lot of shortcomings and deficiencies:

（1）Thin squama schizothoracin poor growth, sexual maturity is slow, and rate of fertilization is low, is unfavorable for schizothoracin amount reproduction；

（2）Environment of hatching is to carry out under field conditions (factors), is susceptible to change, leads to rate of fertilization survival rate, emergence rate and survive Rate is relatively low, is extremely unfavorable for the cultivation of schizothoracin.

Content of the invention

The technical problem to be solved in the present invention is：It is directed to deficiency of the prior art, provide a kind of thin squama of Yalongjiang River to split Abdomen fish artificial fecundation releases method, and the method is effectively recovered and protected the thin squama schizothoracin resource of Yalongjiang River, refined for maintaining The ecosystem balance in rice huller river domain has made significant contribution, and the thin squama schizothoracin simultaneously also allowing economic worth high plays more preferably Economic benefit, and, this technique is solid, workable, can carry out large-scale production.

In order to reach foregoing invention purpose, the technical solution used in the present invention is：A kind of Yalongjiang River thin squama schizothoracin is provided Artificial fecundation releases method, including：

（One）Select the thin squama schizothoracin raun of Yalongjiang River physically fit, that gonad development is good and milter as parent fish, using fiber crops Liquor-saturated dose of MS-222 60 ~ 120mg/L anaesthetizes to the parent fish selecting, and injects oxytocic drug, injection then at the pectoral fin root of parent fish Twice, the time interval of double injection is 36 hours, after per injection at 16 DEG C of water temperature effect 90 hours；Wherein, milter is urged Produce the half that drug dose is raun；Every oxytocic drug used by kg body weight raun is grouped into by the group of following weight：Cyprinus carpio Hypophysis cerebri 3mg+ chorionic-gonadotropin hormone 13 μ g+ luteotropin releasing hormone d-ala analog 1000IU；

（Two）To raun just before giving birth, using the insemination of artificial dry method, and it is gently mixed with feather and makes seminal fluid and ovum mix homogeneously, then plus Enter clear water and continue stirring 30 seconds, stand 5 minutes, then add clean water unsticking, finally put into germ cell in hatching frame Water drenching is hatched；

（Three）Hatch above-mentioned germ cell, Spawning water temperature is 13 ~ 18 DEG C, and indoor hatching adopts fluorescent lamp lighting；

（Four）Germ cell membrane after hatching, enters the larvae cultivation stage；8 ~ 10 days after prelarva membrane, entered with artemia nauplii Row opening feeds；After 13 ~ 15 days, by the Limnodrilus hoffmeisteri mincing and artifical compound feed according to 1:Carry out after 2 weight proportion mixing Raise；After 25-28 days, fed using artifical compound feed completely；

（Five）After fry grows to 2-3 centimetre, control raising the fry density in cylinder in 1-1.1 ten thousand tails/m², every morning, Noon and evening respectively feed once, and the standard that feeds is showed a small surplus by feedstuff and is defined, and often continuous feeding, after 7 days, stops feeding one day；

（Six）After fry grows to 3-5 centimetre, proceed to postlarva breeding phase；Fry is proceeded in big specification cultivation fish pond and is flowed Water is raised, and arranges fountain type water inlet in above-mentioned fishpond, and water inlet is located at 4-6m directly over fishpond, directly sprinkles in waterfall shape It is spilled into pond, water depth 70-100cm, water pH value is 6.5-6.8；Fry density is 500,000 tails/mu；Using artifical compound feed Fed, feeding number of times is every morning, noon, afternoon and evening four times, and the standard that feeds is showed a small surplus by feedstuff and is defined；

（Seven）After postlarva grow to total length be more than 7 centimetres after, carry out artificial releasing；Anesthetis MS-222 20 ~ 40 is adopted when releasing It is calm that/L carries out anesthesia to fry, it is to avoid postlarva mutually extrudes in response to swashing and collision causes body injures and deaths, is released with improving Survival rate afterwards.

Step（One）In, the consumption of anesthetis MS-222 is 60mg/L.

Step（Three）In, the actual conditions of incubating oosperm is：

Will be through step（Two）Process germ cell 13 ~ 18 DEG C of water temperature, the depth of water be 40cm, dissolved oxygen be more than 8mg/L, flow velocity For hatching in the hatchery of 1.0 ~ 1.5m/s 3 ~ 4 days, then flow velocity is down to 0.6 ~ 0.8m/s and hatches 3-5 days.

Step（Three）In, the actual conditions of incubating oosperm is：

Will be through step（Two）Process germ cell 15 DEG C of water temperature, the depth of water be 40cm, dissolved oxygen be 9mg/L, flow velocity be 1.0m/ Hatch in the hatchery of s 4 days, then flow velocity is down to 0.6m/s and hatches 3 days.

The invention has the advantages that：

The Yalongjiang River thin squama schizothoracin artificial fecundation of the present invention release method pass through using parent fish select, carry out having be directed to refined Rice huller river domain in the oxytocic drug injection of thin squama schizothoracin, artificial insemination, incubating oosperm, larvae cultivation, postlarva cultivation and Transport releases seven steps it is achieved that the enhancement releasing of Yalongjiang River thin squama schizothoracin, can not only recover the thin squama of Yalongjiang River and split abdomen Fingerling group, improves the stock number of Yalongjiang River thin squama schizothoracin, and the thin squama schizothoracin of the high Yalongjiang River of value can be made to play more Good economic benefit, has huge using value.

By preparing the oxytocic drug with specific components and content, while not injuring thin squama schizothoracin, also effectively Promote the gonad development of male and female parent fish completely, make the heat simultaneously of most of parent fishs simultaneously, improve the efficiency of artificial induced spawning, more favorably In advanced fry cultivation and management work.

By using specific method for incubating oosperm, substantially increasing incubation rate and the survival rate of thin squama schizothoracin, being The large scale cultivating of thin squama schizothoracin is had laid a good foundation.

Cultivation fish pond is arranged to staged by the present invention, arranges fountain type water inlet, cultivation fish pond profit in fishpond simultaneously With itself drop, water is directly fallen in pond as waterfall, not only avoid big flow to enter water and impact is produced to fry, reduce Fry physical ability is lost, but also greatly increases water dissolved oxygen amount, it is to avoid in breeding process, anoxia phenomenon occurs, and effectively increases fish The survival rate of Seedling cultivation.

The inventive method achieves the enhancement releasing of Yalongjiang River thin squama schizothoracin, can not only effectively reduce key water control project Impact to thin squama schizothoracin population, and contribute to the recovery of its wild resource amount, provide for its biological study simultaneously Basic data, has great economy and environmental ecological benefit.

Brief description

Fig. 1 is the structural representation of cultivation fish pond.

1st, fountain type water inlet；2nd, fishpond.

Specific embodiment

With reference to specific embodiment, the specific embodiment of the present invention is described in detail：

Embodiment 1

Taking the thin squama schizothoracin enhancement releasing technique in Yalongjiang River kwan-yin rockfish class enhancement releasing station as a example, this releases the present embodiment Stand and make great efforts through for many years, technique is released to thin squama schizothoracin artificial fecundation and is studied, captured this kind of artificial fecundation Multiple key technologies in releasing, it will be Yalongjiang River fishery resources and ecological environment that thin squama schizothoracin artificial fecundation releases technique Recovery and protection play more positive effect；It is specially：

Choose in Yalongjiang River capture physically fit, sexually matured thin squama schizothoracin raun and milter as parent fish, using anesthesia Agent MS-222 6mg/L anaesthetizes to the parent fish selecting, and the pectoral fin base portion then at parent fish injects oxytocic drug, injects twice, and two The time interval of secondary injection is 36 hours, after per injection at 16 DEG C of water temperature effect 90 hours；Milter oxytocic drug consumption is The half of raun；Every oxytocic drug used by kg body weight raun is grouped into by the group of following weight：Cyprinus carpio hypophysis cerebri 3mg+ floss Chorionic Gonadotropin 13 μ g+ luteotropin releasing hormone d-ala analog 1000IU；This oxytocic drug helps lend some impetus to male and female parent fish Gonad development completely, make the heat simultaneously of most of parent fishs simultaneously, improve the efficiency of artificial induced spawning, be more beneficial for advanced fry training Educate and management work.

Fully-developed raun after screening abdominal part expands obvious, the athletic injection through oxytocic drug is manually done Method is inseminated, and its gonopore and neighbouring body surface dry towel are wiped clean, and gently squeezes ovum with finger from top to bottom on raun abdominal part top Nest, till ovum grain can not flow out naturally, is clamp-oned milter seminal fluid using same method at once after taking ovum and is used feather gentle agitation 30s, adds a small amount of clear water stirring 30s, stands 5 minutes, finally add a large amount of clean water unstickings, and germ cell is poured into Water drenching hatching in hatching frame；

Incubating oosperm is the process that fertilized fish roe makes the smooth membrane of fry under certain artificial condition, during hatching, using day Light lamp illuminate, and 15 DEG C of water temperature, the depth of water be 40cm, dissolved oxygen be 9mg/L, flow velocity be 1.0m/s hatchery in hatch 4 days, Then flow velocity is down to 0.6m/s to hatch 3 days；

Germ cell membrane after hatching, enters the larvae cultivation stage；After prelarva membrane, 8 ~ 10 days itself yolk absorbs and finishes, and starts Carry out opening feeding with artemia nauplii；After 13 days, by the Limnodrilus hoffmeisteri mincing and artifical compound feed according to 1:2 weight is joined Raised than after mixing；Wherein it is gradually lowered the consumption of Limnodrilus hoffmeisteri with the growth of small fish, after 25 days, adopt hand fit completely Feedstuff is fed.

After fry grows to 2 ~ 3 centimetres, control raising the fry density in cylinder in 1-1.1 ten thousand tails/m², every morning, in Noon and evening respectively feed once, and the standard that feeds is showed a small surplus by feedstuff and is defined, and often continuous feeding, after 7 days, stops feeding one day；

After fry grows to 3 ~ 5cm, proceed to postlarva breeding phase；Fry is proceeded to big specification cultivation fish pond and carries out flowing water raising, fish The high 130cm in pond pond, depth of water 100cm, fishpond flowing water pH value is 6.5；As shown in figure 1, arranging fountain type water inlet 1 in fishpond 2, Fountain type water inlet is located at 4m directly over fishpond, directly splashes into pond in waterfall shape；This fishpond 2 is in that staged is distributed, relatively For usual water inlet is directly accessed culturing pool for duct type, by high-rise tuber drainage to lower floor pond, the mouth of pipe is in this fishpond Flat, therefore, makes water directly fall in pond as waterfall using this itself drop of cultivation platform, on the one hand can avoid Big flow enters the injury that water impacts to fry, reduces the loss of fry physical ability；On the other hand water dissolved oxygen amount can be increased, it is to avoid support During growing, anoxia phenomenon occurs；In this fishpond, fry density is 500,000 tails/mu, and bait adopts artifical compound feed, feeds Number of times is four times, respectively morning, noon, afternoon and evening, and being satiated with food to feed is showed a small surplus as standard with feedstuff；Until fry gives birth to Grow to and enhancement releasing when 7 centimetres, after packing, can be used for.

Transport releases the fry being possessed the condition of releasing and carries out through fountain type water inlet, after oxygen supplement net training, being caught with net It is contained in the fish bag of oxygenation after dragging for, then releases to corresponding location, when releasing, using anesthetis MS-222 20mg/L, fry is entered Row anesthesia is calm, it is to avoid postlarva mutually extrudes in response to swashing and collision causes body injures and deaths, and then raising releases survival rate.

Using the method for the present embodiment, about 2,500,000 tail thin squama schizothoracin original seed fry, warp are released to Yalongjiang River successively Repeatedly validation trial, technique is solid, is at home and abroad pioneering, has significant economic, society and environmental ecology Benefit.

Embodiment 2

Choose in Yalongjiang River capture physically fit, sexually matured thin squama schizothoracin raun and milter as parent fish, using anesthesia Agent MS-222 120/L anaesthetizes to the parent fish selecting, and the pectoral fin base portion then at parent fish injects oxytocic drug, injects twice, The time interval of double injection is 36 hours, after per injection at 16 DEG C of water temperature effect 90 hours；Milter oxytocic drug consumption Half for raun；Every oxytocic drug used by kg body weight raun is grouped into by the group of following weight：Cyprinus carpio hypophysis cerebri 3mg+ Chorionic-gonadotropin hormone 13 μ g+ luteotropin releasing hormone d-ala analog 1000IU；This oxytocic drug helps lend some impetus to male and female parent The gonad development of fish completely, makes the heat simultaneously of most of parent fishs simultaneously, improves the efficiency of artificial induced spawning, is more beneficial for advanced fry Cultivate and management work.

Fully-developed raun after screening abdominal part expands obvious, the athletic injection through oxytocic drug is manually done Method is inseminated, and its gonopore and neighbouring body surface dry towel are wiped clean, and gently squeezes ovum with finger from top to bottom on raun abdominal part top Nest, till ovum grain can not flow out naturally, is clamp-oned milter seminal fluid using same method at once after taking ovum and is used feather gentle agitation 30s, adds a small amount of clear water stirring 30s, stands 5 minutes, be eventually adding a large amount of clean water unstickings, finally put into germ cell Water drenching hatching in hatching frame；

Incubating oosperm is the process that fertilized fish roe makes the smooth membrane of fry under certain artificial condition, during hatching, using day Light lamp illuminate, and 13 DEG C of water temperature, the depth of water be 40cm, dissolved oxygen be 8mg/L, flow velocity be 1.5m/s hatchery in hatch 3 days, Then flow velocity is down to 0.8m/s to hatch 5 days；

Germ cell membrane after hatching, enters the larvae cultivation stage；After prelarva membrane, 8 ~ 10 days itself yolk absorbs and finishes, and uses halogen Worm naupliuss carry out opening feeding；After 15 days, by the Limnodrilus hoffmeisteri mincing and artifical compound feed according to 1:2 weight proportion mixes Raised after conjunction；Wherein it is gradually lowered the consumption of Limnodrilus hoffmeisteri with the growth of small fish, after 28 days, adopt artifical compound feed completely Fed.

After fry grows to 2 ~ 3 centimetres, control raising the fry density in cylinder in 10,000 tails/m², every morning, noon and Respectively feed once in the evening, and the standard that feeds is showed a small surplus by feedstuff and is defined, and often continuous feeding, after 7 days, stops feeding one day；

After fry grows to 3 ~ 5cm, proceed to postlarva breeding phase and enter；Fry is proceeded to big specification cultivation fish pond and carries out flowing water raising, As shown in figure 1, the high 150cm in this fishpond 2 pond, depth of water 70cm, fishpond flowing water pH value is 6.8；Setting fountain type water inlet in fishpond 2 Mouth 1, fountain type water inlet is located at the normal position 6m of fishpond, directly splashes into pond in waterfall shape；This fishpond 2 is divided in staged Cloth, for usual water inlet is directly accessed culturing pool for duct type, this fishpond by high-rise tuber drainage to lower floor pond, The mouth of pipe is in flat, therefore, using this itself drop of cultivation platform, water is directly fallen in pond as waterfall, on the one hand may be used To avoid big flow to enter the injury that water impacts to fry, reduce the loss of fry physical ability；On the other hand water dissolved oxygen amount can be increased, Avoid the anoxia in breeding process；In this fishpond, fry density is 500,000 tails/mu, and bait adopts artifical compound feed, feeds Number of times is four times, respectively morning, noon, afternoon and evening, and being satiated with food to feed is showed a small surplus as standard with feedstuff；Until fry gives birth to Grow to and enhancement releasing when 7 centimetres, after packing, can be used for.

Transport releases the fry being possessed the condition of releasing and carries out through fountain type water inlet, after oxygen supplement net training, being caught with net It is contained in the fish bag of oxygenation after dragging for, then releases to corresponding location, when releasing, using anesthetis MS-222 40mg/L, fry is entered Row anesthesia is calm, it is to avoid postlarva mutually extrudes in response to swashing and collision causes body injures and deaths, and then raising releases survival rate.

Using the method for the present embodiment, about 3,000,000 tail thin squama schizothoracin original seed fry, warp are released to Yalongjiang River successively Repeatedly validation trial, technique is solid, is at home and abroad pioneering, has significant economic, society and environmental ecology Benefit.

Although being described in detail to the specific embodiment of the present invention in conjunction with specific embodiments, it is not to this The restriction of scope of patent protection.In claims limited range, those skilled in the art is without creative work The various modifications that can make or adjustment are still protected by this patent.

Claims (4)

1. the schizothoracin artificial fecundation of Yalongjiang River thin squama releases method, it is characterized in that, including：

（One）Select the thin squama schizothoracin raun of Yalongjiang River physically fit, that gonad development is good and milter as parent fish, using fiber crops Liquor-saturated dose of MS-222 60 ~ 120mg/L anaesthetizes to the parent fish selecting, and injects oxytocic drug, injection then at the pectoral fin root of parent fish Twice, the time interval of double injection is 36 hours, after per injection at 16 DEG C of water temperature effect 90 hours；Wherein, milter is urged Produce the half that drug dose is raun；Every oxytocic drug used by kg body weight raun is grouped into by the group of following weight：Cyprinus carpio Hypophysis cerebri 3mg+ chorionic-gonadotropin hormone 13 μ g+ luteotropin releasing hormone d-ala analog 1000IU；

（Two）To raun just before giving birth, using the insemination of artificial dry method, and it is gently mixed with feather and makes seminal fluid and ovum mix homogeneously, then plus Enter clear water and continue stirring 30 seconds, stand 5 minutes, then add clean water unsticking, finally put into germ cell in hatching frame Water drenching is hatched；

（Three）Hatch above-mentioned germ cell, Spawning water temperature is 13 ~ 18 DEG C, and indoor hatching adopts fluorescent lamp lighting；

（Four）Germ cell membrane after hatching, enters the larvae cultivation stage；8 ~ 10 days after prelarva membrane, entered with artemia nauplii Row opening feeds；After 13 ~ 15 days, by the Limnodrilus hoffmeisteri mincing and artifical compound feed according to 1:Carry out after 2 weight proportion mixing Raise；After 25-28 days, fed using artifical compound feed completely；

（Five）After fry grows to 2-3 centimetre, control raising the fry density in cylinder in 1-1.1 ten thousand tails/m², every morning, in Noon and evening respectively feed once, and the standard that feeds is showed a small surplus by feedstuff and is defined, and often continuous feeding, after 7 days, stops feeding one day；

（Six）After fry grows to 3-5 centimetre, proceed to postlarva breeding phase；Fry is proceeded in big specification cultivation fish pond and is flowed Water is raised, and arranges fountain type water inlet in above-mentioned fishpond, and water inlet is located at 4-6m directly over fishpond, directly sprinkles in waterfall shape It is spilled into pond, water depth 70-100cm, water pH value is 6.5-6.8；Fry density is 500,000 tails/mu；Using artifical compound feed Fed, feeding number of times is every morning, noon, afternoon and evening four times, and the standard that feeds is showed a small surplus by feedstuff and is defined；

（Seven）After postlarva grow to total length be more than 7 centimetres after, carry out artificial releasing；Anesthetis MS-222 20 ~ 40 is adopted when releasing It is calm that/L carries out anesthesia to fry, it is to avoid postlarva mutually extrudes in response to swashing and collision causes body injures and deaths, is released with improving Survival rate afterwards.

2. Yalongjiang River short hairs schizothoracin artificial fecundation according to claim 1 releases method, it is characterized in that, step（One） In, the consumption of anesthetis MS-222 is 60mg/L.

3. Yalongjiang River short hairs schizothoracin artificial fecundation according to claim 1 releases method, it is characterized in that, in step（Three） In, the actual conditions of incubating oosperm is：

Will be through step（Two）Process germ cell 13 ~ 18 DEG C of water temperature, the depth of water be 40cm, dissolved oxygen be more than 8mg/L, flow velocity For hatching in the hatchery of 1.0 ~ 1.5m/s 3 ~ 4 days, then flow velocity is down to 0.6 ~ 0.8m/s and hatches 3-5 days.

4. Yalongjiang River short hairs schizothoracin artificial fecundation according to claim 3 releases method, it is characterized in that, in step（Three） In, the actual conditions of incubating oosperm is：

Will be through step（Two）Process germ cell 15 DEG C of water temperature, the depth of water be 40cm, dissolved oxygen be 9mg/L, flow velocity be 1.0m/ Hatch in the hatchery of s 4 days, then flow velocity is down to 0.6m/s and hatches 3 days.