CN101960996A - Artificial breeding method of Scaphesthes macrolepis fry - Google Patents

Artificial breeding method of Scaphesthes macrolepis fry Download PDF

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Publication number
CN101960996A
CN101960996A CN2010105276630A CN201010527663A CN101960996A CN 101960996 A CN101960996 A CN 101960996A CN 2010105276630 A CN2010105276630 A CN 2010105276630A CN 201010527663 A CN201010527663 A CN 201010527663A CN 101960996 A CN101960996 A CN 101960996A
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fry
parent population
spawning
fertilized egg
laying nest
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俆绍刚
王跃智
杨晓飞
杨贵强
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Beijing Fisheries Research Institute
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Beijing Fisheries Research Institute
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention provides an artificial breeding method of Scaphesthes macrolepis fry, comprising: selecting sexual-maturity Scaphesthes macrolepis parent fish older than 3 years, and making sets at the ratio of female to male of 1:2; keeping the put density of parent fish in a spawning pond to be 6-8 sets/m<2>; putting one spawning nest for every 10 sets of parent fish, and laying coarse sand of which the average thickness is 4-6 cm in the spawning nest for the parent fish to spawn and unstick; in the spawning season, injecting oxytocic hormone into the parent fish to induce the parent fish to spawn and release sperm; making an incubation groove by battens and bolting silk; putting oosperm into the incubation groove to hatch in still water, wherein the dissolved oxygen in the incubation groove is more than 5mg/L, the illumination intensity is more than 1500-3000Lux, and the water temperature is 20-22 DEG C; after demoulding, temporarily breeding in the incubation groove; and after the fry floats up and flatly swims, introducing the fry into a plastic bag filled with water to be oxygenated and packaged or introducing the fry spawning pond. The method of the invention can intensively obtain the Scaphesthes macrolepis fry which favourably develops on batch, has simple operation and high parent fish fertility rate, is favourable for realizing large-scale production of Scaphesthes macrolepis and has an important meaning for protecting and culturing the Scaphesthes macrolepis wild resource on large scale.

Description

The artificial breeding method of a kind of many squama shovel jaw fish fries
Technical field
The present invention relates to the artificial breeding method of fresh-water fishes, specifically, relate to the large-scale artificial mating system of a kind of many squama shovel jaw fish fries, belong to aquatic products healthy aquaculture applied technical field.
Background technology
Many squama shovel jaw fishes have another name called many squamas onychostoma simus, are distributed in the middle and upper reaches of Jia Lingjiang River water system and river Han water system, upstream, Huaihe River, Weihe River water system, Yi He, Luo He and tai shan.Be the small-sized economic fish of living in a kind of preciousness in the mountain stream water small stream that absolute elevation is a 300-1500 rice, many squama shovel jaw fishes are fine and tender taste not only, and delicious not raw meat is simultaneously well-known with its nutritious and unique medicinal health value.In recent years owing to many reasons such as weather arid, tourism development, overfishing and water environment artificial pollutions; the living environment of many squama shovel jaw fishes is seriously damaged; the living space is more and more littler; wild quantity is fewer and feweri; Beijing Juma River water system is endangered, at present in Shandong, ground such as Shaanxi, Gansu classify many squamas shovel jaw fishes as provincial watching for animals.Waterman author has carried out more research to the many squama shovels of artificial propagation jaw fish in recent years, existing many squama shovel jaw fish artificial propagation techniques have been done more detailed research at parent population selection, ocyodinic selection, hatching m, seed rearing, a kind of easier many squama shovel jaw fry kind mating systems are provided, but this method exists the parent population utilization ratio not high, the ocyodinic result of use is not good, can not form batch process, hatch shortcomings such as survival rate is low, be difficult to adapt to the demand of extensive artificial propagation.
Summary of the invention
The present invention is intended to overcome the shortcoming of existing many squama shovel jaw fish fry artificial breeding technologies, and the method for the many squama shovels of a kind of artificial breeding jaw fish fry is provided.
In order to realize the object of the invention, the artificial breeding method of a kind of many squamas shovel jaw fish fries of the present invention comprises that parent population selections, ocyodinic injection, laying nest setting, parent population lay eggs, fertilized egg collection, incubating oosperm, fry is foster temporarily and the fry collection.Its technical scheme is: (1) parent population is selected: reach 16 ℃ and carry out parent fish selection when stablize in water temperature, press 1: 2 combo of female-male proportion, require that sexually matured many squamas shovel the jaw fishes as parent population more than 3 ages.Female selection sexual gland physically well develops, specification more than 80 gram/tails, no sick and wounded, do not have cultivating of deformity as the single pond of parent population; Milter selects gently to be extruded with that seminal fluid flows out, specification more than 50 gram/tails, no sick and wounded, do not have deformity as the pond cultivation of parent population list.(2) ocyodinic injection: when water temperature reaches 18 ℃ and hasten parturition when being stabilized in more than 18 ℃.Female milter is all adopted a shot, and raun medicine and dosage are LHRH-A 20.4 μ g/ tail+human chorionic gonadtropin (HCG) 20 units/tail, milter reduces by half; Before the injection, oxytocic hormone dilutes with 0.9% physiological saline, and the injection site is the muscle of base portion behind the dorsal fin, and the miter angle that is bordering on the fish body injects.(3) laying nest setting: as laying nest, per 10 groups of parent populations are placed 1 laying nest, 30-40 centimetre of drum diameter with plastics or irony drum, 20-30 centimetre of height, drum bottom tiling grains of sand diameter is at the rough sand of 2 millimeter, and thickness is 4-6 centimetre, is used for the parent population place of laying eggs.(4) parent population lays eggs: put into spawning pond after parent population is induced through hastening parturition and raise together with, parent population gets final product natural spawning, and raun lays eggs when laying eggs, and milter row is smart, finishes the fertilization of ovum simultaneously, treats that raun swims when leaving away to the front upper place, and outstanding husky sedimentation is embedded in fertilized egg in the sand.(5) fertilized egg is gathered: laying nest is taken out from spawning pond, is to collect fertilized egg under the condition of 1500-3000Lux at light intensity.Laying nest is added spawning pond water to 80% of laying nest volume, gently stir rough sand with have gentle hands, treat that ovum pours in the sky basin after the come-up in the rough sand immediately, repeatable operation can all be collected ovum in the laying nest several times.(6) fertilized egg hatching: make the framework of incubation tank with batten, 40 mesh sieve thin,tough silk as the bottom of incubation tank and are all around put into cement pit with incubation tank, and cement pit requires more than the depth of water 50cm, and specification is at 3m 2More than, pond water dissolved oxygen is greater than 5mg/L, and intensity of illumination remains on 1500-3000Lux, renders in the off-the-shelf incubation tank after egg white is washed, and it is 0.5/cm that fertilized egg is put into density 2, the hydrostatic hatching is adopted in hatching, and the hatching water temperature is 20-22 ℃, must keep the static of ovum between the incubation period, in time rejects dead ovum, and brooding time is 70-90h.(7) fry is supported temporarily: behind the fry demoulding, need to support temporarily in incubation tank, can not put into the fry rearing pond immediately, it supports the same fertilized egg hatching of condition temporarily, support time 100-120h temporarily, during supporting temporarily incubation tank is changed about 1/2 new water, changing the water front and back temperature difference can not be above 2 ℃.(8) fry is collected: a jiao of incubation tank is mentioned, treat fry concentrate on another angle of incubation tank after available bailer directly fry is scooped out, be incorporated with in the plastic sack of water the oxygenation packing or import the fry rearing pond and cultivate.
The present invention can obtain well-developed many squama shovel jaw fish fries, and easy and simple to handle.Adopt raun: the ratio of milter=1: 2, improved the fertilization rate of fish-egg, solved the not high problem of fertilization rate under the natural spawning condition; The laying nest method to set up is simple, is convenient to parent population and lays eggs, and is beneficial to the unsticking of fish-egg simultaneously; The employing hormone is hastened parturition, and can concentrate acquisition fish-egg in batches, and it is long to have solved the parent population egg-laying time, the problem that fry is not concentrated; Hydrostatic hatching, control intensity of illumination have significantly improved the incubation rate of fish-egg, and the large-scale production of shoveling jaw fish fries for many squamas provides a kind of more efficient methods.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Embodiment
When water temperature reaches 16 ℃, select parent population in many squama shovel jaw fishes of cultivating, selection criteria is: 3 ages, above sexually matured many squamas shoveled the jaw fishes, and female selection belly obviously expands, gonad development is good, specification more than 80 gram/tails, no sick and wounded, do not have deformity as the pond cultivation of parent population list; Milter selects gently to be extruded with that seminal fluid flows out, specification more than 50 gram/tails, no sick and wounded, do not have deformity as the pond cultivation of parent population list.Press 1: 2 combo of female-male proportion.
The pool water within degree of depth is in the spawning pond of 0.8-1.0 rice laying nest to be set, and per 10 groups of parent populations are placed 1 laying nest, and tiling thickness in laying nest bottom is 4-6 centimetre rough sand, is used for the parent population place of laying eggs.When water temperature reaches 18 ℃ and when being stabilized in more than 18 ℃, with the parent population injection oxytocic hormone that chooses, put into spawning pond, putting fish density is 6-8 group parent population/m 2Female milter is all adopted a shot, and the injected dose of oxytocic hormone is: raun LHRH-A 20.4 μ g/ tail+human chorionic gonadtropin (HCG) 20 units/tail, milter reduces by half.Before the injection, oxytocic hormone dilutes with 0.9% physiological saline.
Injecting method is: the injection site is the muscle of base portion behind the dorsal fin, and needle forward is miter angle in the fish body, and depth of needle is 1cm.
Put into spawning pond after parent population is induced through hastening parturition and raise together with, parent population gets final product natural spawning, and raun lays eggs when laying eggs, and milter row is smart, finishes the fertilization of ovum simultaneously, treats that raun swims when leaving away to the front upper place, and outstanding husky sedimentation is embedded in fertilized egg in the sand.
Water temperature is at 18-24 ℃, and after injection that afternoon, second day early morning, parent population promptly can lay eggs, because many squamas shovel jaw fish belongs to the type of laying eggs in batches, parent population can be placed in the spawning pond always, up to the end of laying eggs.Keep miniflow water in the spawning pond, oxygen in water is greater than 5mg/L, and spawning pond is kept quite around reaching, in case parent population gets a fright.The parent population egg-laying time focused mostly in the morning, also had afternoon a small amount of parent population to lay eggs.Laying nest is since 5:00 in early morning, and every 2h extracts once, but afternoon, 3h extracted once.After laying nest takes out from spawning pond, need under intensity of illumination is the condition of 1500-3000Lux, to collect fish-egg.Laying nest is added spawning pond water to 80% of laying nest volume, gently stir rough sand with have gentle hands, treat that fertilized egg pours in the sky basin after the come-up in the rough sand immediately, repeatable operation can all be taken out ovum several times.
Fish-egg is put into clear water and is cleaned after taking out from laying nest, removes the unfertilized egg of white, cleans sand and other foreign material, puts into incubation tank and hatches, and it is 0.5/cm that fish-egg is put into density 2Incubation tank is for to make framework with batten, 40 mesh sieve thin,tough silk as the bottom of incubation tank and all around, specification is length * wide * height: 30cm * 30cm * 15cm.Hatching preincubation groove will clean, sterilize; Incubation tank is put into cement pit, and cement pit requires more than the depth of water 50cm, and specification is at 3m 2More than, pond water dissolved oxygen is greater than 5mg/L, and intensity of illumination remains on 1500-3000Lux, adopts the hydrostatic hatching, and the hatching water temperature is 20-22 ℃, must keep the static of ovum between the incubation period, in time rejects dead ovum, and incubation rate 91% is finished in hatching behind the 70-90h.
Behind the fry demoulding, can not put into the fry rearing pond immediately, need in incubation tank, to support temporarily, supporting condition temporarily is that intensity of illumination remains on 1500-3000Lux, hydrostatic, and water temperature is 20-22 ℃, dissolved oxygen needs cement pit is changed about 1/2 new water during supporting temporarily greater than 5mg/L, supports time 100-120h temporarily.
When whole fry can level moves about behind the 120h, can carry out the collection of fry, method is for mentioning a jiao of incubation tank, treat that fry concentrates on another angle of incubation tank after, directly fry is scooped out with bailer, be incorporated with in the plastic sack of water the oxygenation packing or import the fry rearing pond and cultivate.
Though above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.

Claims (8)

1. the artificial breeding method of squama more than kind shovel jaw fish fry comprises that parent population selections, ocyodinic injection, laying nest setting, parent population lay eggs, fertilized egg collection, incubating oosperm, fry is foster temporarily and the step of collection, it is characterized in that:
(1) parent population is selected: select above sexual maturity parent population in 3 ages, press 1: 2 combo of female-male proportion;
(2) ocyodinic injection: at spawning season with exogenous hormone LHRH-A 2Be injected in the parent population body with human chorionic gonadtropin;
(3) laying nest setting: the non-covered container that is paved with rough sand with the bottom is as laying nest, and it is in the spawning pond of 0.8-1.0m that laying nest is placed depth of water;
(4) parent population lays eggs: put into spawning pond after parent population is induced through hastening parturition and raise together with, parent population gets final product natural spawning, and raun lays eggs when laying eggs, and milter row is smart, finishes the fertilization of ovum simultaneously, treats that raun swims when leaving away, and outstanding husky sedimentation is embedded in fertilized egg in the sand;
(5) fertilized egg is gathered: wash laying nest repeatedly with Chi Shui, and stir rough sand and collect fertilized egg;
(6) incubating oosperm: make incubation tank and place in the pond with batten and bolting silk, fertilized egg is put into the hatching of incubation tank hydrostatic;
(7) fry is supported temporarily and collects: still foster temporarily in incubation tank behind the fry demoulding, treat the flat trip of its come-up back derivation.
2. method according to claim 1 is characterized in that parent population is chosen as: more than raun cabrage 80 grams, belly expands, and dorsal fin has the red nuptial coloration of four strips, does not have star-pursuing on the anal fin, and gonopore has mastoid process; More than milter cabrage 50 grams, dorsal fin has the red nuptial coloration of six strips, and star-pursuing is arranged on the anal fin, and gonopore is not outstanding, and light squeezing has seminal fluid to flow out.
3. method according to claim 1 is characterized in that injection of hormone dosage is: raun LHRH-A 20.4 μ g/ tail+human chorionic gonadtropin 20 units/tail, milter reduces by half; Before the injection, hormone dilutes with 0.9% physiological saline, and the injection site is the muscle of base portion behind the dorsal fin, and the miter angle that is bordering on the fish body injects.
4. method according to claim 1, it is characterized in that laying nest is set to: with plastics or irony drum as laying nest, diameter 30-40 centimetre, 20-30 centimetre of height, drum bottom tiling grains of sand diameter is the rough sand of 1.5-2.5 millimeter, and thickness is 4-6 centimetre.
5. method according to claim 1 is characterized in that fertilized egg is gathered and is: laying nest is taken out from spawning pond, add Chi Shui to 80% of its volume in laying nest, stir rough sand, treat that fertilized egg come-up back in the rough sand derives.
6. method according to claim 1 is characterized in that incubating oosperm is: fertilized egg is rendered in the incubation tank, and it is 0.5/cm that fertilized egg is thrown in density 2, adopting the hydrostatic hatching, the hatching water temperature is 20-22 ℃, and the incubation tank intensity of illumination remains on 1500-3000Lux, and brooding time is 70-90h.
7. method according to claim 1 is characterized in that temporary the supporting of fry is: behind the fry demoulding, need carry out supporting temporarily, support the incubation condition of condition with ovum temporarily, foster temporarily time 100-120h in incubation tank.
8. method according to claim 1 is characterized in that fry is collected as: incubation tank is mentioned for one jiao, treat directly fry to be scooped out after fry concentrates on another angle of incubation tank, be incorporated with in the plastic sack of water the oxygenation packing or import the fry rearing pond and cultivate.
CN2010105276630A 2010-10-27 2010-10-27 Artificial breeding method of Scaphesthes macrolepis fry Pending CN101960996A (en)

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102283152A (en) * 2011-07-12 2011-12-21 北京市水产科学研究所 Method for breeding fry of scaphesthes macrolepis
CN103609503A (en) * 2013-12-13 2014-03-05 蒲德成 Ecology-imitating cultivating breeding method for parent fishes of scaphesthes macrolepis
CN105638533A (en) * 2016-03-04 2016-06-08 镇坪县天源生态养殖有限公司 Parent fish culture method for varicorhinus macrolepis
CN105746398A (en) * 2016-03-04 2016-07-13 镇坪县天源生态养殖有限公司 Hatching method for fries of scaphes macrolepis
CN105766709A (en) * 2016-03-04 2016-07-20 镇坪县天源生态养殖有限公司 Adult fish breeding method of varicorhinus macrolepis
CN106942096A (en) * 2016-12-01 2017-07-14 重庆两江生态渔业发展有限公司 A kind of many squamas shovel the artificial propagation breeding method of jaw fish
CN107258629A (en) * 2017-08-18 2017-10-20 西南大学 It is a kind of to improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization
CN107333680A (en) * 2016-04-29 2017-11-10 岚皋县绿水生态农业有限责任公司 A kind of artificial breeding method of more squama shovel jaw fishes and special hatchery
CN107396861A (en) * 2017-08-18 2017-11-28 西南大学 A kind of method of the more squama shovel jaw fish artificial induced spawnings in southwest
CN111316936A (en) * 2018-12-13 2020-06-23 射阳县周正海家庭农场 Static water stripping artificial propagation method for large-scale barbel fish

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Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102283152A (en) * 2011-07-12 2011-12-21 北京市水产科学研究所 Method for breeding fry of scaphesthes macrolepis
CN103609503B (en) * 2013-12-13 2015-11-18 蒲德成 Many squamas onychostoma simus parent population Ecology culturing and reproducing
CN103609503A (en) * 2013-12-13 2014-03-05 蒲德成 Ecology-imitating cultivating breeding method for parent fishes of scaphesthes macrolepis
CN105746398B (en) * 2016-03-04 2018-10-30 镇坪县天源生态养殖有限公司 A kind of fry hatching method of more squama shovel jaw fishes
CN105638533A (en) * 2016-03-04 2016-06-08 镇坪县天源生态养殖有限公司 Parent fish culture method for varicorhinus macrolepis
CN105746398A (en) * 2016-03-04 2016-07-13 镇坪县天源生态养殖有限公司 Hatching method for fries of scaphes macrolepis
CN105766709A (en) * 2016-03-04 2016-07-20 镇坪县天源生态养殖有限公司 Adult fish breeding method of varicorhinus macrolepis
CN105766709B (en) * 2016-03-04 2018-11-02 镇坪县天源生态养殖有限公司 A kind of adult fish rearing method of more squama shovel jaw fishes
CN105638533B (en) * 2016-03-04 2018-10-12 镇坪县天源生态养殖有限公司 A kind of parent population cultural method of more squama shovel jaw fishes
CN107333680A (en) * 2016-04-29 2017-11-10 岚皋县绿水生态农业有限责任公司 A kind of artificial breeding method of more squama shovel jaw fishes and special hatchery
CN106942096A (en) * 2016-12-01 2017-07-14 重庆两江生态渔业发展有限公司 A kind of many squamas shovel the artificial propagation breeding method of jaw fish
CN107258629A (en) * 2017-08-18 2017-10-20 西南大学 It is a kind of to improve the method that many squamas shovel jaw artificial propagation fish rate of fertilization
CN107396861A (en) * 2017-08-18 2017-11-28 西南大学 A kind of method of the more squama shovel jaw fish artificial induced spawnings in southwest
CN111316936A (en) * 2018-12-13 2020-06-23 射阳县周正海家庭农场 Static water stripping artificial propagation method for large-scale barbel fish

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