CN104472412A - Coilia ectenes step type breeding method - Google Patents

Abstract

A stepwise breeding method for scorpion anchovy, comprising ecological imitation cultivation of backup parent ponds, intensive cultivation of parent ponds, parent selection for induced production, parental induction, natural fertilization, collection of fertilized eggs, incubation of fertilized eggs, intensive seedling cultivation in indoor cement ponds, It is composed of 9 production steps for large-scale seed cultivation in outdoor ponds. It is characterized in that adult cuttlefish above the age of 2 are used as backup parents for artificial breeding, and the natural ecological breeding model is adopted; good parents are selected Transport it into the oxytocin tank. After the parental oxytocin is induced, the eggs are collected and counted and then transferred to the hatching pool. After the fertilized eggs are collected, they are put into the hatching pool with water. After 5 to 6 days after the membrane ruptures, the fry open their mouths and feed them through a 80-mesh sieve. Egg yolk and small-sized freshwater rotifers obtained by filtering; after 15 days of cultivation, separate ponds are started, and when the full length of fry reaches 20-30mm, they are put into outdoor ponds for seedling cultivation.

Description

A kind of stepwise breeding method of sword anchovy

technical field

The invention relates to aquaculture, in particular to a method for breeding silverfish.

Background technique

Coilia nasus Schlegel, also known as coilia nasus Schlegel, commonly known as saury and hair saury, is a migratory fish in rivers and seas, mainly distributed in the Yellow Sea, Bohai Sea and East China Sea in China. It is distributed in all rivers leading to the sea, and the lower reaches of the Yangtze River have the highest production . Anchovy from the Yangtze River is famous for its tender meat, fresh and fat, and strong seasonality. It is also known as the "three delicacies of the Yangtze River" together with shad and puffer fish in the Yangtze River. The resources of anchovy in the Yangtze River are extremely rich in history. In recent years, due to the influence of many factors such as overfishing and the deterioration of the ecological environment, the resources of the anchovy have declined sharply, the output has shown a downward trend year by year, and the individual miniaturization is serious. The anchovy can no longer form a dominant population in the Yangtze River. The price of commercial fish is also rising. The breakthrough and promotion of the artificial breeding technology of sword anchovy, and then promote the industrialized production of this fish, can promote the adjustment of the fishery industry structure, promote the efficiency of fishery and increase the income of fishermen. Therefore, as a new species of artificial breeding, the sword anchovy has a good development prospect.

At present, there are two main breeding methods for the anchovy: one is the pond ecological breeding method, that is, after the pond is disinfected, an appropriate amount of breeding-age parents is put in to allow them to mate, lay eggs, and hatch naturally. The seedlings are also cultivated. In this pond, the ecological cultivation of the pond is carried out, the water is changed in an appropriate amount, and there is very little manual intervention. When the fry reach a certain size, the net is drawn to estimate the number. Systematic training is required, but this method has a very low survival rate of seedlings. It relies entirely on natural breeding, and the unit output is very small. Sometimes there are no surviving fry in some ponds, so the overall number of seedlings obtained is also small; the other It is an intensive breeding method in indoor cement pools, that is, artificial induction of labor in indoor cement pools, artificial incubation of fertilized eggs in indoor cement pools, and indoor cultivation of seedlings in cement pools. The advantage of this method is that artificial breeding is completely carried out under artificial control conditions, and the survival rate of seedling cultivation It is relatively high and can obtain batches of seedlings. However, in the middle and later stages of seedling cultivation, due to the growth of individual fry, the food intake will increase greatly. At this time, a large amount of live bait is required, and a large amount of labor is required to catch the live bait from the pond. Put it into the indoor cement pond, and at the same time, the stocking density of large-scale fish fry in the cement pond should be greatly reduced, which will occupy a large amount of indoor cement pond, and also require a lot of labor to do tedious feeding, bottom suction, water change, and pond turning and other tedious seedling operations.

Contents of the invention

The purpose of the present invention is to provide a method for stepwise breeding of sword anchovy to adapt to the large-scale and intensive breeding and production of sword anchovy.

The technical scheme of the present invention consists of ecological imitation cultivation of backup parent ponds, intensive cultivation of parent ponds, parent selection for induced labor, parental induced labor, natural fertilization, collection of fertilized eggs, incubation of fertilized eggs, intensive seedling cultivation in indoor cement ponds, and large-scale outdoor ponds. Seedling cultivation consists of 9 production steps, and its characteristic is that adult cuttlefish above the age of 2 from artificial breeding is used as the backup parent of artificial breeding, and it is cultivated in the outer pond every year from May to October in the Gregorian calendar, and the parent adopts Simulate the natural ecological cultivation mode: Thoroughly disinfect the pond before stocking, and disinfect with 20ppm bleaching powder with water. Filter with double-layer sieve tulle bags, use 80-mesh sieve for the inner layer, and 60-mesh sieve for the outer layer. After entering the water for a week, the water color is slightly yellowish green, and the transparency is 20-30cm. 200 tails/mu, put 3Kg small-sized white prawns per mu, and inoculate mysis; feed once a day, the bait is shrimp pellet feed, and feed white prawns, change the water once a month, and use local estuary Brackish water with a salinity of 5-8; move the net to the pond in November-December of the Gregorian calendar to remove wild fish and harmful organisms in time, and thoroughly disinfect the pond before moving the pond. The method is the same as that of the parent before stocking. It is 50-100 tails/mu. At this time, the pond water is used, and the salinity is 10-15. The amount of small-sized white prawns in the pond is increased to 6 Kg per 667m ² . Ecological cultivation; from February to March of the following Gregorian calendar, the water in the pond will gradually be replaced with fresh water; from March to May of the Gregorian calendar, regular stretching exercises, usually 2-3 times; When the temperature reaches 20-25°C, the abdomen of the parent female fish gradually expands. At this time, the parent for oxytocin can be selected. The standard for selecting the parent is: the abdomen of the female fish is enlarged and soft, and the cloaca is reddish; A small amount of semen flows out and does not disperse when it meets water; the selected parents are transported into the oxytocin pool in drums with a volume of 100L, and 5-10 parents are placed in each barrel; the entire selection and transportation process should be handled gently without leaving the water; oxytocin The pool adopts a cement pool of 10-20m ² , the water depth is 1.1-1.2m, the stocking density of induced spawning parents is 1-2 tails/m ² , continuous micro-inflation, shading and controlling the light to be below 500Lux; the ratio of females to spawning broodstock is 2/3-3 /4, the water temperature for oxytocin is 22～24℃; the oxytocin hormone is luteinizing hormone releasing hormone A _{2 ,} that is, LHRH-A ₂ , the hormone is dissolved in 0.9% normal saline, and it is prepared and used immediately; the oxytocin dose: female fish 20- 30ug/Kg, male fish halved, 0.5-2.0ml of normal saline for oxytocin per fish; after oxytocin of the parents, keep quiet around the oxytocin tank, adopt natural fertilization method, effect time is generally 18-35 hours, keep water temperature 22-22 At 24°C, send special personnel to observe the mating of the parents to determine the spawning time. During mating, the male fish chases the female fish, and some fish will skim over the water surface, after which eggs can be found in the pool; after spawning 2 Within 4 hours, the fertilized eggs should be collected in time, otherwise the fertilized eggs will rupture the membrane. When collecting the fertilized eggs, stop the air in the oxytocin pool for 10-20 minutes, let the fertilized eggs float on the water surface, and pull the net with a 80-mesh soft sieve. When netting, the lower end of the net is 30-40cm away from the bottom of the pool, so that the parent anchovy can escape the net smoothly. The egg membrane of the anchovy is very thin, and the fertilized eggs cannot be squeezed out of the water. When pulling the net, it must be operated with water. , pause for 3-5 minutes, let the flipped eggs float to the water surface again, and then pull the net again. After pulling the net 3-5 times, collect and count the eggs and transfer them to the hatching pool. Generally, each spawning pool can get 50,000-200,000 Fertilized eggs; fertilized eggs are hatched with temperature control, static water, and aeration. The hatching water is fresh water filtered with 200 mesh sieves to prevent medium-sized copepods and cladocera from entering the water body. After the fertilized eggs are collected, they are put into 10- 20m ³ seed cultivation pond, the seed cultivation pond is used as the hatching pond, the hatching density is 5000-10000 grains/m ³ , inflated for 24 hours for hatching, the density of air stones is 1.0-1.5/m ² , and the water temperature is controlled at 23~ At 24°C, after 28 to 40 hours of hatching, the fry can break the membrane; use static water aeration and oxygenation indoor cement ponds for cultivation, and the water for seedlings will be filtered with 150-mesh sieve after two stages of sedimentation in the pond and reservoir; The air stone is 0.5-1.2 pieces/m ² , the air inflation is microwave-like, and the water temperature is controlled at 23-26°C; use low water level seedlings, first inject 2/3 of the pool water, then add water every day, fill it up after 3 days, and then change it every day Water 1/4, from the 6th day after planting, suck the dirt and clean the bottom once a day, change the water every other day at a rate of 50%, and from the 15th day after planting, change the water every other day at a rate of 60-60% 70%; 5 to 6 days after membrane rupture, the fry opened their mouths, and fed egg yolk and small freshwater rotifers filtered through an 80-mesh sieve; the density of rotifers was 3 to 5/mL. Supplement the feeding amount of medium-sized rotifers after Tianhou to increase its palatability. After 20 days of cultivation, the total length of the fry is 20mm at this time, and their active feeding ability is already strong. Therefore, the bait is mainly fed with large rotifers, medium-sized cladocerans and copepods. For the larvae of the species, the zooplankton were fished and filtered with a 40-mesh sieve and then fed. They were fed once in the morning and afternoon. At the same time, Artemia nauplii were supplemented to increase the nutrition of the bait; Turn over the original pond, after the pond is divided, the density will be 1000-2000 tails/m ³ , and then turn over the pond once every 7-10 days; use 60-mesh soft sieve tulle to pull the net for the separation and turning of the pond, and the netting process should be slow , The seedlings are operated with water to avoid unnecessary damage. Large-scale fry in the cement pool; after 25-35 days of general fry cultivation, when the total length of the fry reaches 20-30mm, the fry's activity ability is enhanced at this time, and the active predation ability is strong. Fish fry can be put into ponds outside for seed cultivation; choose earthen ponds in the estuary area with a flat bottom of the pond. The ponds are preferably 2m deep, 1.5m water level, and 3-5 mu in area. Each pond is equipped with a 1.5kw aerator. The outlet is covered with a double-layer 60-mesh sieve tulle bag for filtration, and the outlet is used with a 60-mesh gate. After the pond is disinfected for 2 days, enter Soak the water until the bottom of the pond is submerged, drain thoroughly after soaking for 48 hours and expose to the sun for later use; according to the seedling release time, enter the water 5-10 days in advance, and use local natural fresh water for the inflow, the advanced water is 50cm, and then according to the water color and the biomass of the bait Gradually add water until the water level reaches 1-1.5m. During the period of making ponds, the aerator will be turned on for 1 hour at noon in fine weather, and basically not turned on at night. Generally, when the water temperature is 25-30°C, the color of the water will become thicker after 3-5 days. There will be a small amount of food organisms such as rotifers, small cladocerans and copepod larvae. If the number is very small, it can be inoculated by fishing from ponds where other food organisms are rich. After 3-5 days, use a beaker to inoculate Sampling at multiple points in the pond to check the bait density, wait until the biomass of the bait in the pond reaches 20-40/L, then release the seedlings; choose the morning of fine weather, release the seedlings in the upper wind of the pond, and turn on the aerator in advance before the fry go down the pond To make the pool water not stratified and the bait organisms distributed evenly, use a 100-liter large drum, put 30 liters of water in the shading transport, and put 200-300 tails in each bucket. Fry adaptation, after 2-3 minutes, the fry are released, the stocking density is generally 4000-6000 fish/mu; after the fry is released, the aerator will be turned on for 1 hour at noon on a sunny day and 8-12 hours at night, and the start-up time will be increased in bad weather; Change 1/3 of the water every half a month, and check the biological density of the pond bait once every 3-5 days. If the biological density of the bait is found to be lower than 1/L, fish the biological bait from the exogenous pond and replenish it in time; Cultivate for 15-20 days. The average size of the fry is 6.0-6.5cm in body length and 1.00-1.05g in weight. At this time, the fin rays and scales of the fry have been fully developed, and the fry can be placed in the juvenile stage. It is conventionally farmed in ponds or sold as summer fingerlings.

The outstanding features of the present invention relative to the prior art are: 1) It improves two methods for the breeding of anchovy, highlights the advantages of the two methods, solves the problems of the two methods, and greatly creates an overall improvement in seedling cultivation. The survival rate reduces the time for intensive cultivation of seedlings in indoor cement ponds (about 35 days), improves the turnover rate of indoor cement ponds, improves the specifications of indoor cultivation ponds (body length 6.0-6.5cm), and reduces the tediousness of indoor cultivation The operation has greatly improved the survival rate (85-95%) and unit yield (4000-5500 tails/mu) of the pond cultured anchovy; the method is simple and practical, suitable for large-scale breeding and production of the anchovy, and is easy to be adopted by people. Accept and promote; 2) Solve the problem of the intensive breeding method of indoor cement ponds for cultivating cuttlefish fry, the shortage of biological bait in the later stage, the consumption of a lot of labor, the long time for indoor seedling cultivation (50-75 days), and the small size of the pool (body length 4 -5cm); 3) Solve the problem of low survival rate (0-10%) and very little unit yield (1000-2000 tails/mu) of seedlings cultivated by pond ecological breeding method.

Detailed ways

Production technique of the present invention comprises the steps:

1. Imitation ecological cultivation of backup parent ponds: artificially bred offspring of 2-year-old cuttlefish are used as backup parents for artificial breeding. They are cultured in external ponds from May to October in the Gregorian calendar every year. The parents use simulated natural ecology Cultivation mode: the size of the cultivation pond is 3-5 mu, the pond is thoroughly disinfected before stocking, and sterilized with 20ppm bleaching powder with water. After 2-3 days, the water at the bottom of the pond is pumped out, and then sterilized with 300 kg/mu of quicklime dry pond, and exposed to the sun After one week of water intake, the water is filtered with a double-layer sieve tulle bag. The inner layer is 80 mesh and the outer layer is 60 mesh sieve. After a week of water inflow, the water color is slightly yellowish green, and the transparency is 20-30cm. It can be stocked in the Yangtze River. The stocking density is 200 tails/mu, and the stocking density is 200 tails/mu. Put 3Kg of small-sized white prawns per mu to inoculate mysis; it is enough to feed once a day, and the bait is pelleted feed for shrimp, mainly for white prawns. Feed, change water once a month, use local estuary brackish water (salinity 5-8).

2. Intensive cultivation of parental ponds: Move the nets to move the ponds from November to December in the Gregorian calendar, and remove wild fish and harmful organisms in time. The size of the ponds is 2-3 mu. Before moving the ponds, the ponds should be thoroughly disinfected. The method is the same as that of the parents Disinfect the pond, stocking density is 50-100 tails/mu, at this time, the salinity of the pond water is increased to 10-15, and the amount of small-sized white prawns in the pond is increased to 6 Kg per ^667m2 , and the water and bait are changed and fed. The method is the same as that of the backup parent pond for ecological cultivation; from February to March of the following year, the water in the pond is gradually replaced with fresh water; from March to May of the Gregorian calendar, regular exercise is carried out, usually 2-3 times.

3. Selection of parents for oxytocin: In the middle and early May of the Gregorian calendar, when the pond water temperature reaches 20-25°C, the abdomen of the parent female fish gradually expands. At this time, the parents for oxytocin can be selected. The cloaca is reddish; for male fish, a small amount of semen flows out when the cloaca is lightly pressed, and it does not disperse when it meets water; the selected parents are transported in drums (100L) and placed in the oxytocin tank, and 5-10 parents are placed in each bucket; During the entire selection and transportation process, the operation should be gentle and never leave the water.

4. Oxygenated parents: 10-20m ² round or square cement ponds are used for the oxytocin pool, the water depth is 1.1-1.2m, the stocking density of the oxytocin parents is 1-2 tails/m ² , continuous micro-inflation, shading control (below 500Lux) . The sex ratio of broodstock for oxytocin is 2/3~3/4, and the water temperature for oxytocin is 22~24℃; the oxytocin hormone is luteinizing hormone releasing hormone A ₂ (LHRH-A ₂ ), which is dissolved in 0.9% normal saline, and is now prepared Currently used; oxytocin dose: 20-30ug/Kg for female fish, half for male fish, 0.5-2.0ml of normal saline for oxytocin.

5. Natural fertilization: After the parent induces labor, keep quiet around the spawning pool, adopt natural fertilization method, the effect time is generally 18-35 hours, keep the water temperature at 22-24°C, and send special personnel to observe the mating of the parents to determine the spawning time. , the male fish chases the female fish, and some fish will skim the water surface, after which eggs can be found in the pool.

6. Collection of fertilized eggs: Within 24 hours after laying eggs, collect fertilized eggs in time, otherwise fertilized eggs will rupture the membrane. Pull the net with soft sieve silk net. When pulling the net, the lower end of the net is about 30-40cm away from the bottom of the pool, so that the parent anchovy can escape the net smoothly. The egg membrane of the anchovy is very thin, and the fertilized eggs cannot be squeezed out of the water. To operate with water, after each pulling the net, stop for 3-5 minutes, let the flipped eggs float to the water surface again, and then pull the net again, after pulling the net 3-5 times, more than 95% of the eggs can be collected and counted before transfer In the hatching pool, generally, each spawning pool can obtain 50,000 to 200,000 fertilized eggs.

7. Incubation of fertilized eggs: fertilized eggs are hatched with temperature control, still water and aeration. The hatching water is fresh water filtered with 200 mesh sieves to prevent medium-sized copepods and cladocera from entering the water body. After the fertilized eggs are collected, they will be brought with water Put it into the seed cultivation pond (10-20m ³ ), use the seed cultivation pond as the hatching pond, the hatching density is 5000-10000 grains/m ³ , inflate for 24 hours for hatching, and the density of air stones is 1.0-1.5/m ² , the water temperature is controlled at 23-24°C, and the fry can be ruptured after 28-40 hours of hatching.

8. Intensive cultivation of seedlings in indoor cement pools: static water aeration and oxygenation are used for cultivation, and the water for seedlings is filtered by 150-mesh sieve silk after two-stage sedimentation in ponds and reservoirs; seedling cultivation pools (10-20m ³ ), diffuse air 0.5-1.2 stones/m ² , the amount of air inflated is microwave-like, and the water temperature is controlled at 23-26°C; use low-water level seedlings, first inject 2/3 of the pool water, then add water every day, fill it up after 3 days, and then change it every day Water 1/4, from the 6th day after planting, suck the dirt and clean the bottom once a day, change the water every other day at a rate of 50%, and from the 15th day after planting, change the water every other day at a rate of 60-60% 70%; 5 to 6 days after the seedlings were planted, the fry opened their mouths, and the egg yolks and small freshwater rotifers filtered through an 80-mesh sieve were fed; Supplement the feeding amount of medium-sized rotifers after Tianhou to increase its palatability. After 20 days of cultivation, the total length of the fry is 20mm at this time, and their active feeding ability is already strong. Therefore, the bait is mainly fed with large rotifers, medium-sized cladocerans and copepods. For the larvae of the species, the zooplankton were fished and filtered with a 40-mesh sieve and then fed. They were fed once in the morning and afternoon, and some Artemia nauplii were supplemented at the same time to increase the nutrition of the bait.

After 15 days of cultivation, the ponds were divided, and the original ponds were turned over at the same time. After the division, the density was 1000-2000 fish/m ³ , and then the ponds were turned once every 7-10 days. Use 60-mesh soft sieve tulle to pull the net for sub-pond and pond turning. The process of pulling the net should be slow, and the seedlings should be operated with water to avoid unnecessary damage. Large-sized fish fry in the cement pool.

Generally, after 25-35 days of fry cultivation, the total length of the fry reaches 20-30mm. At this time, the activity ability of the fry is enhanced and the ability to actively prey is strong. The fry can be placed in outdoor ponds for seed cultivation.

9. Cultivation of large-scale seedlings in outdoor ponds: choose earthen ponds in the estuary area with a flat bottom. The ponds are preferably 2m deep, 1.5m water level, and 3-5 mu in area. Each pond is equipped with a 1.5kw aerator. The outlet is covered with a double-layer 60-mesh sieve tulle bag for filtration, and the outlet is used with a 60-mesh gate. After the pond is disinfected for 2 days, enter the water until the bottom of the pond is submerged and soak. After soaking for 48 hours, it is completely drained and exposed to the sun for use; Water intake 5-10 days in advance, the water intake uses local natural fresh water, the advanced water is 50cm, and then gradually add water according to the water color and bait biomass until the water level reaches 1-1.5m. At night, it is basically closed. Generally, when the water temperature is 25-30°C, the color of the water will become thicker after 3-5 days. If the quantity is very small, it can be inoculated by fishing from other ponds rich in bait organisms. After 3-5 days, use a beaker to take samples at multiple points in the pond to test the bait density, and wait until the bait biomass in the pond reaches 20-40/L. Release the seedlings; in the morning when the weather is fine, release the seedlings in the upwind of the pond. Before the fry enter the pond, turn on the aerator in advance to make the water not stratified and the bait organisms distributed evenly. Use a 100-liter large drum (30 liters of water) Shade transportation, put 200-300 fish in each bucket, dump the bucket first when releasing seedlings, let the water in the pond enter the bucket first, let the fry adapt, and release the fry after 2-3 minutes, the stocking density is generally 4000-6000 fish / mu; after the seedlings are released, the aerator will be turned on for 1 hour at noon on a sunny day, and 8-12 hours at night, and the time will be increased if the weather is bad; change the water by 1/3 every half a month, and check the biological density of the pond bait every 3-5 days Once, if the biological density of the bait is found to be lower than 1/L, the biological bait should be taken from the exogenous pond to replenish in time; the pond is cultivated for 15-20 days, and the average size of the fry is 6.0-6.5cm in body length and 1.00 in weight. -1.05g, the survival rate is 85-95%. At this time, each fin ray and scales of the fry have been fully developed and entered the juvenile stage. The fry can be put into ponds for conventional culture or sold as summer fish fingerlings.

Through this method, the Fengxian Base of the Shanghai Fisheries Research Institute has obtained 80,000 large-scale anchovy seedlings in ponds, with an average body length of 6.2-6.5cm, an average weight of 1.00-1.05g, and a survival rate of 85-95% for pond seedling cultivation. %, the unit yield of the pond is 35000-55000 tails/mu.

Claims (1)

1. A stepwise breeding method for cuttlefish, which consists of ecological imitation cultivation in the pond of the reserve parent, strengthening cultivation in the pond of the parent, selection of the parent for oxytocin, oxytocin of the parent, natural fertilization, collection of fertilized eggs, hatching of fertilized eggs, intensive seedlings in indoor cement ponds Cultivation and large-scale seed cultivation in outdoor ponds are composed of 9 production steps. It is characterized in that the artificially bred offspring of the 2-year-old anchovy adult fish is used as the backup parent of artificial breeding, and it is carried out from May to October in the Gregorian calendar every year. Breeding in external ponds, the parents adopt the model of simulating natural ecological cultivation: the ponds are thoroughly disinfected before stocking, and 20ppm bleaching powder is used for disinfection with water. After 2 days, the bottom water of the ponds is pumped out, and then 300 kg/mu of quicklime is used for disinfection of the dry ponds. After a week of exposure to the sun Water intake is filtered by double-layer sieve tulle bags, with 80-mesh sieve for the inner layer and 60-mesh sieve for the outer layer. After entering the water for a week, the water color is yellowish green, and the transparency is 20-30cm. Reserve parents, the stocking density is 200 tails/mu, put 3Kg small-sized white prawns per mu, and inoculate mysis; feed once a day, the bait is pelleted feed for shrimp, and feed the white prawns to eat, change every month Water once, using local estuary brackish water with a salinity of 5-8; move the net to move the pond in November-December of the Gregorian calendar, remove wild fish and harmful organisms in time, and thoroughly disinfect the pond before moving the pond. The method is the same as that of the parent. Disinfect the former pond, stocking density is 50-100 tails/mu, at this time, the salinity of the pond water is 10-15, the amount of small-sized white prawns in the pond is increased to 6 Kg per 667m ² , change water, bait and feeding method Imitate ecological cultivation in the same pond as the backup parent; from February to March of the following year, the water in the pond will gradually be replaced with fresh water; from March to May of the Gregorian calendar, regular net-drawing exercises, usually 2-3 times; until May of the Gregorian calendar In the middle and early ten days, when the water temperature in the pond reaches 20-25°C, the abdomen of the parent female fish will gradually expand. At this time, the parent fish for induction of labor can be selected. There is a small amount of semen flowing out from the cloacal hole, and it does not disperse when it meets water; the selected parents are transported into the oxytocin pool in drums with a volume of 100L, and 5-10 parents are placed in each drum; Gentle and inseparable from the water; the oxytocin pond adopts a cement pool of 10-20m ² , the water depth is 1.1-1.2m, the stocking density of oxytocin parents is 1-2 fish/m ² , continuous micro-inflation, shading and adjusting the light to be below 500Lux; the sex ratio of oxytocin broodstock is 2/3～3/4, the water temperature for oxytocin is 22～24℃; the oxytocin hormone is luteinizing hormone releasing hormone A _2, the hormone is dissolved in 0.9% normal saline, and it is prepared and used now; the oxytocin dose: female fish 20- 30ug/Kg, male fish halved, 0.5-2.0ml of normal saline for oxytocin per fish; after oxytocin of the parents, keep quiet around the oxytocin tank, adopt natural fertilization method, effect time is generally 18-35 hours, keep water temperature 22-22 At 24°C, send a special person to observe the mating of the parents to determine the time of spawning. During mating, the male fish chases the female fish, and some fish will pass by the water surface, after which eggs can be found in the pool; within 24 hours after spawning , it is necessary to collect fertilized eggs in time. When collecting fertilized eggs, stop the air in the oxytocin pool for 10-20 minutes, let the fertilized eggs float on the water surface, and use 80-mesh soft sieve silk net to pull the net. When pulling the net, the lower end of the net is 30-40cm away from the bottom of the pool. , so that the parent Anchovy can escape the net smoothly. The egg membrane of the Anchovy is very thin, and the fertilized eggs cannot be squeezed out of the water. When pulling the net, it must be operated with water. After each pulling the net, stop for 3-5 minutes to let the flipping Eggs float to the surface of the water again, and then pull the net again. After pulling the net 3-5 times, the eggs are collected and counted and then transferred to the hatching pool; Fresh water, to prevent medium-sized copepods and cladoceras from entering the water body. After the fertilized eggs are collected, put them into the seed cultivation pond with water, and use the seed cultivation pond as the hatching pond. The hatching density is 5,000-10,000 grains/m ³ , 24 The hatching is aerated within 1 hour, the density of air stones is 1.0-1.5/m ² , the water temperature is controlled at 23-24°C, and the fry can be ruptured after 28-40 hours of hatching; the still water aerated and aerated indoor cement pool is used for cultivation, and the water for seedlings is passed through After two-stage sedimentation in ponds and reservoirs, filter them with 150-mesh sieves; in the seedling cultivation pools, the air stones are 0.5-1.2 pieces/m ² , the aeration volume is microwave-like, and the water temperature is controlled at 23-26°C; For seedlings, first inject 2/3 of the pool water, then add water every day, fill it up after 3 days, then change the water 1/4 every day, from the 6th day after the seedlings are planted, suck the dirt and clean the bottom once a day, and change the water once every other day From the 15th day after the seedlings are planted, the water change is 60-70% every other day; 5-6 days after the membrane rupture, the fry open, and the egg yolk and small fresh water filtered through an 80-mesh sieve are fed. Rotifers; the density of rotifers is 3-5/mL, feed once in the morning and afternoon, supplement the feeding amount of medium-sized rotifers after 10 days of cultivation to increase its palatability. Therefore, the bait is mainly fed the larvae of large rotifers, medium-sized cladocerans and copepods. After the zooplankton are caught, they are filtered with 40-mesh sieves and then fed. They are fed once in the morning and afternoon, and supplemented Feed Artemia nauplii to increase the nutrition of the bait; after 15 days of cultivation, divide the ponds, and turn over the original ponds at the same ^time . ; Use 60-mesh soft sieve silk net to pull the net for sub-pond and pond turning. The net-pulling process should be slow, and the seedlings should be operated with water to avoid unnecessary damage to large-scale fry in the cement pool; after 25-35 days of general fry cultivation, when The total length of the fry reaches 20-30mm. At this time, the activity ability of the fry is enhanced and the ability to actively prey is strong. The fry are put into the outside pond for seedling cultivation; choose an earthen pond in the estuary area with a flat bottom of the pond. The pond is 2m deep, the water level is 1.5m, and the area 3-5 acres, each pond is equipped with a 1.5kw aerator, the water inlet is covered with a double-layer 60-mesh sieve tulle bag for filtration, and the outlet is used with a 60-mesh gate. After the pond is disinfected for 2 days, enter the water until the bottom of the pond is submerged Soak, after soaking for 48 hours, drain thoroughly and expose to the sun for use; according to the seedling release time, water in 5-10 days in advance, water in Use local natural fresh water, advance water 50cm, and then gradually add water according to the water color and bait biomass until the water level reaches 1-1.5m. At 30°C, after 3-5 days, the color of the water will become thicker. At this time, a small amount of rotifers, small cladocerans and copepod larvae will appear in the water body. After 3-5 days, use a beaker to test at multiple points in the pool. The bait density, wait until the bait biomass in the pond reaches 20-40/L, then the seedlings can be released; choose the morning of fine weather, release the seedlings in the upper wind of the pond, and turn on the aeration machine in advance before the fry go to the pond to prevent the water from stratifying And bait biological distribution is uniform, use a 100-liter large drum, put 30 liters of water in the shade for transportation, put 200-300 tails in each bucket, dump the bucket first when releasing seedlings, let the water in the pond enter the bucket first, and let the fry adapt, 2- After 3 minutes, the fish fry are released, and the stocking density is generally 4000-6000 fish/mu; after the seedlings are released, the aerator will be turned on for 1 hour at noon on a sunny day, and 8-12 hours at night, and the start time will be increased if the weather is bad; change every half a month Water 1/3, check the biological density of the pond bait once every 3-5 days, if the biological density of the bait is found to be lower than 1/L, fish the biological bait from the exogenous pond and replenish it in time; cultivate the pond for 15-20 days The average size of the fry is 6.0-6.5cm in body length and 1.00-1.05g in weight. At this time, the fin rays and scales of the fry have been fully developed, and they have entered the juvenile stage. The fry can be put into ponds for conventional breeding Or as a summer flower fish species.