CN105379650B - A kind of method for obtaining batch America hilsa herring fertilized eggs - Google Patents
A kind of method for obtaining batch America hilsa herring fertilized eggs Download PDFInfo
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- CN105379650B CN105379650B CN201510789940.8A CN201510789940A CN105379650B CN 105379650 B CN105379650 B CN 105379650B CN 201510789940 A CN201510789940 A CN 201510789940A CN 105379650 B CN105379650 B CN 105379650B
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- 235000013601 eggs Nutrition 0.000 title claims abstract description 36
- 241000252203 Clupea harengus Species 0.000 title claims abstract description 30
- 241001110084 Hilsa Species 0.000 title claims abstract description 30
- 235000019514 herring Nutrition 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 120
- 238000004519 manufacturing process Methods 0.000 claims abstract description 50
- 239000004568 cement Substances 0.000 claims abstract description 37
- 230000001737 promoting effect Effects 0.000 claims abstract description 24
- 235000019688 fish Nutrition 0.000 claims abstract description 21
- 241000251468 Actinopterygii Species 0.000 claims abstract description 20
- 230000004720 fertilization Effects 0.000 claims abstract description 18
- 238000009395 breeding Methods 0.000 claims abstract description 12
- 230000001488 breeding effect Effects 0.000 claims abstract description 12
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 claims abstract description 12
- 229920002554 vinyl polymer Polymers 0.000 claims abstract description 12
- 239000002985 plastic film Substances 0.000 claims abstract description 11
- 229920006255 plastic film Polymers 0.000 claims abstract description 11
- 239000013505 freshwater Substances 0.000 claims abstract description 10
- 150000003839 salts Chemical class 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 7
- 239000008188 pellet Substances 0.000 claims abstract description 7
- 238000009423 ventilation Methods 0.000 claims abstract description 5
- 230000017448 oviposition Effects 0.000 claims abstract description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 24
- 108010000912 Egg Proteins Proteins 0.000 claims description 24
- 210000004681 ovum Anatomy 0.000 claims description 24
- 238000001556 precipitation Methods 0.000 claims description 12
- 239000013049 sediment Substances 0.000 claims description 11
- 238000005276 aerator Methods 0.000 claims description 8
- 210000001015 abdomen Anatomy 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 235000019750 Crude protein Nutrition 0.000 claims description 4
- 238000006213 oxygenation reaction Methods 0.000 claims description 4
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 claims description 3
- 241000851180 Palaemon carinicauda Species 0.000 claims description 3
- 239000007844 bleaching agent Substances 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 2
- 210000003608 fece Anatomy 0.000 claims description 2
- 239000012535 impurity Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000003442 weekly effect Effects 0.000 claims description 2
- 241000269793 Cryothenia peninsulae Species 0.000 claims 1
- 230000008021 deposition Effects 0.000 claims 1
- 230000017525 heat dissipation Effects 0.000 claims 1
- 239000002574 poison Substances 0.000 claims 1
- 231100000614 poison Toxicity 0.000 claims 1
- 230000001007 puffing effect Effects 0.000 claims 1
- 230000000630 rising effect Effects 0.000 abstract description 2
- 238000009360 aquaculture Methods 0.000 description 5
- 244000144974 aquaculture Species 0.000 description 5
- 230000013011 mating Effects 0.000 description 5
- 241001482107 Alosa sapidissima Species 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000008239 natural water Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 235000021401 pellet diet Nutrition 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000002349 well water Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
A kind of method for obtaining batch America hilsa herring fertilized eggs, it is characterized in that standby parent's overwintering culture is carried out using vinyl house cement pit, using local fresh water, salinity is stepped up when changing water, salinity reaches 12 15 ‰ to the end, overwintering culture water temperature is maintained at 8 DEG C or more, bait is commercial No. 3 material of fresh-water fishes expanded pellet feed, at the beginning of 4 months by the end of March, into parent's spring reinforced cultivating, with the rising of temperature, using the ventilation of vinyl house both sides, plastic film, capping shade film are removed, keeps 16 18 DEG C of water temperature in cement pit；Every time when changing water, gradually reduce salinity, each salt reduction degree 23 ‰, to the end salinity reach local river water, at the beginning of 6 months mid-Mays, select to promote production and be put into circuit of promoting production with parent, promote production 0.8~1 tail of parent's breeding density/m2, using natural spawning fertilization mode, fertilized eggs are collected in time after parent's oviposition, to be operated with water, hatchery is moved on to after counting.
Description
Technical field
The present invention relates to aquaculture, especially a kind of method for obtaining batch America hilsa herring fertilized eggs.
Background technology
America hilsa herring (Alosa sapidissima) is commonly called as Alosa sapidissima, is distributed mainly on North America Atlantic Ocean west bank from adding
It puts on airs in Quebec to the Florida State river in the U.S. and ocean.America hilsa herring system breeding migration fish, plump big and fleshy, meat is fresh
Delicious, the very high economic value of tool is introduced a fine variety extensively all over the world.At home, Shanghai Aquatic Product Institute took the lead in 1998
From the U.S., Mississippi introduces a fine variety the wild produced fertilized eggs of America hilsa herring, then carries out incubating oosperm, seed rearing and supports
It grows.Due to America hilsa herring because, meat similar to the shape of Chinese hilsa herring compares favourably, in the case where the Changjiang river hilsa herring is endangered, America
Hilsa herring is also very popular, and the market price is also very high, and 200-300 members/jin, raiser cultivates the fish, the seedling of the fish one after another
Kind has formed vast market prospect.Due to the unique reproductive habit of the fish, at present, America hilsa herring breeding cultivation is per family using pumping
Take below 150 meters of underground, winter-spring season water temperature stability 16-17 DEG C phreatic water come carry out the parent of the fish cultivation, Ran Houzhi
The mode that fertilized eggs are collected in parent's aquaculture pond is connected on, but Deep Underground water resource is limited, also has region limitation, Er Qiehao
More coastal cities have limited Deep Underground water for aquaculture, and existing are collected in the hilsa herring adult fish culture pond of America
The mode technical parameter of fertilized eggs compare blindly, contingency it is larger, these limit America hilsa herring seeds batch supply and rule
Modelling produces.Therefore, it is badly in need of a set of technology, in the case where maintaining water temperature condition without phreatic water, river water or river using natural water area
Saliva is to complete America hilsa herring parent culture, promote production natural mating and fertilization collection etc., to obtain the fertilized eggs of batch, to adapt to U.S.
The batch supply of continent sapidissima fingerlings.
Invention content
Problem to be solved of the present invention is to provide a kind of method for obtaining batch America hilsa herring fertilized eggs.
Technical scheme of the present invention is selected by standby parent overwintering culture, parent's spring reinforced cultivating, the parent that promotes production, parent
Promote production, natural spawning fertilization and fertilization ovum collecting production stage composition, it is characterized in that by the 2 ages more than America hilsa herring of artificial breeding into
Standby parent of the fish as artificial propagation selects in the annual 11-12 months healthy and strong, build grossness, weight 0.75-1.5Kg
Commodity adult fish enters standby parent's overwintering culture, and standby parent is cultivated using vinyl house cement pit, using pool wall is smooth, area
For 150-250m2Rectangular or round cement pit, deep 1.2-1.8m, cement pit top setting bow-shaped ring top, top covering light transmission are protected
Warm plastic film, with the shade membrane cover of shading rate 90% on a plastic film, light regulating reduce light transmittance, while according to pond
Water water colour adjusts the hiding rare of shade film, and hiding rare maintains 30%-40%；Gas head is placed in cement pit, gas head density is
0.2-0.3/m2, with Roots blower continuous charge in 24 hours, tolerance is with boiling-like；Cement pit is sterilized before putting in a suitable place to breed, water inlet
20cm, full pool spilling head after being dissolved with 20PPm bleaching powder, after impregnating 48 hours, draining is rinsed well, is dried 3-4 days and is reused；It chooses
When the standby parent of choosing puts in a suitable place to breed, using local fresh water, latter week is put in a suitable place to breed, when changing water every time, step up salinity, rise salinity every time
2-3 ‰, to the end salinity reach 12-15 ‰, rise salt and overwintering culture use locality salinity with water as ‰ estuarine waters of 10-15,
The useful water of institute is used by pond level-one precipitation, vinyl house cement pit nature sunlight pre-heating temperature elevation and two-stage precipitation, then
It is filtered with 80 mesh sieve tulles, changes water weekly 1 time, every time 2/3, every 25-35 days, sediment upturning 1 time, overwintering culture water temperature was maintained at 8
DEG C or more, water temperature difference is changed less than 2 DEG C, and sediment upturning water temperature difference is less than 1 DEG C；Bait is commercial No. 3 material of fresh-water fishes expanded pellet feed, slightly
Protein content is 38%, is fed daily 1 time, is subject to 1-2 hours and has ingested；It sinks to alleviate culture pond bottom excreta and residual bait
Product is corrupt and pollutes water quality, puts exopalaemon carinicauda in a suitable place to breed in culture pond, stocking rate is 5-10 jins/pond；At the beginning of -4 months by the end of March, into parent
In the reinforced cultivating stage in this spring, water temperature has just started to reach 15-16 DEG C in vinyl house cement pit at this time, upper with outside temperature
It rises, using the ventilation of vinyl house both sides, dismounting plastic film, capping first layer shade film, is capped second layer shade film again, keeps
16-18 DEG C of water temperature in cement pit；When changing water every time, salinity is gradually reduced, each salt reduction degree 2-3 ‰, salinity, which reaches, to the end works as
Ground river water, salt reduction and reinforced cultivating are with water using local river water, and the useful water of institute is using big by pond level-one precipitation, shade
Canopy cement pit Temperature fall and two-stage precipitation, then filtered with 80 mesh sieve tulles, per changing within 5-6 days water 1 time, every time 2/3, every 2 weeks
Sediment upturning 1 time changes water temperature difference less than 2 DEG C, and sediment upturning water temperature difference is less than 1 DEG C；Bait is commercial No. 3 material of fish expanded pellet feed,
Crude protein content is more than 40%, feeds daily 2 times, is subject to 1-2 hours and has ingested；Other management are the same；To mid-May -6
At the beginning of the month, 17-19 DEG C of water temperature in the greenhouse culture pond that shades at this time, parent's raun abdomen expands successively, can select use of promoting production at this time
Parent, criteria for selection：Raun postabdomen expands and soft, and cloacal aperture is micro- red, some gently press abdomen to have ovum outflow；Milter is light to press
Cloacal aperture has a small amount of sperm to flow out, and meets water and do not dissipate；The parent of select is transported using 100L drums and is put into circuit of promoting production, every barrel
Put 2-3 tail parents；Entire select will be softly from water with operation in transportational process；Circuit of promoting production using 30.0m × 5.0m ×
1.2m rectangle cement pits, depth of water 1.0m, by constructing intermediate isolating wall and smearing round pond corner, formation 60.0m × 2.4m ×
1.1m ellipses are closed circuit, carry out oxygenation using 0.75KW waterwheel aerators and form flow, using 10KW air energy thermals
Pump hot water machine group keeps water temperature stability in circuit by cold and hot heat-dissipating pipe, and bow-shaped ring top, top are set for top in cement pit
The shade film of three layers of shading rate 90% is covered, ensures the intensity of illumination on daytime less than 300Lux, parent's breeding density of promoting production is 0.8
~1 tail/m2, sex ration 1:1.1~1:1.3, water temperature of promoting production is 17.5~19 DEG C；After parent enters circuit of promoting production, to keep
It is absolutely quiet around spawning inducing pool, using natural spawning fertilization mode, circuit water velocity is remained closed as 0.07~0.11m/s,
Water temperature of promoting production is 17.5~19 DEG C；A small amount of expanding compound feed is fed in promoting production pond, with reduce parent absorb fertilized eggs, but will and
When remove residual bait, otherwise expanded pellet diet impregnate in water the time grown and will sink to the bottom, be mixed into fertilized eggs, cause fertilized eggs cleaning and
It is difficult to detach impurity；America hilsa herring parent has the custom for fertilized eggs of ingesting, and to collect fertilized eggs in time after parent's oviposition, collect fertilization
Before ovum, suspend waterwheel aerator, after flow rate of water flow is allowed to lower, pond at circuit dead angle is destroyed with the soft bolting silk triangle diddle-net of 24 mesh is flat
Bottom, America hilsa herring egg membrane is very thin, and fertilized eggs cannot be squeezed from water, and collecting ovum will operate with water, after copying ovum every time, be then turned on aerator
3-5 minutes, ovum automatic turning is allowed then to copy ovum again at circuit dead angle, it, can be by more than 95% ovum collecting, so after being repeated 3 times
It clears up afterwards, move on to hatchery after counting.
The present invention is relative to the outstanding feature of the prior art：1) during standby parent's overwintering culture, in natural situation
The lower water temperature using sunlight and plastic film heating cultivation water in the case where being heated without the energy, keeps temperature of cultivation 8
DEG C or more；During parent's spring reinforced cultivating, when natural temperature is higher than 18 DEG C, reduced using ventilation and covering shade film
The water temperature of cultivation water keeps cultivating water temperature less than 18 DEG C；During promote production, reduced and maintenance water using air energy unit
Water temperature；Therefore, it during entire parent culture and breeding, is maintained without using phreatic water (16-17 DEG C of winter-spring season) heating and cooling
The water temperature of parent culture water, also unused phreatic water make breeding water, this protect it is valuable descend deep-well water resource cleanly,
Also save the energy；2) using the river mouth brackish water of local natural water area during standby parent's overwintering culture, this is rationally utilized
Abundant brackish water resource, which save clean freshwater resources；3) seine for a long time moves what pond was taken exercise during parent culture
In the case of, using select and move the preferable parent of gonad development focus on promote production circuit natural spawning mating fertilization method,
This has many good qualities, such as than the existing method that fertilized eggs are collected directly in parent's aquaculture pond；In circuit of promoting production, development
Preferable female male parent meets, and rate is higher, and the probability of natural mating is also relatively high, also prevents sexual gland and do not develop into dried fish disturbing
Parent's natural mating and the produced fertilized eggs of intake；It also avoids simultaneously due to pollutant and residual bait phase in conventional parent's aquaculture pond
To difficulty more, that the fertilized eggs being collected into cleaning is caused to detach；4) large batch of America hilsa herring can be obtained using this technical solution
Fertilized eggs, operation is more easy, and method is more practical, also improves the quality of fertilized eggs and prelarva indirectly, is suitble to America hilsa herring scale
Change breeding production, and be easy to be accepted and promote.
Description of the drawings
Fig. 1 is that America hilsa herring parent promotes production the structure diagram of circuit.
Specific embodiment
The present invention is made of following production stage：
1st, standby parent overwintering culture：Using the 2 ages more than America hilsa herring adult fish of artificial breeding as the standby parent of artificial propagation
This, selects healthy and strong, build grossness, the commodity adult fish of weight 0.75-1.5Kg enters standby parent and gets in the annual 11-12 months
Winter cultivates, and cultivates standby parent using simple plastic greenhouse cement pit, uses rectangular or round cement pool area as 150-
250m2, deep 1.2-1.8m, pool wall is smooth；Setting bow-shaped ring top above cement pit, top covering transparent heat insulating plastic film, is used
On a plastic film, light regulating reduces light transmittance, while is adjusted and shaded according to water water colour the shade membrane cover of shading rate 90%
The hiding rare of film, is typically maintained in 30%-40%；Gas head is placed in cement pit, gas head density is 0.2-0.3/m2, use Roots
Air blower continuous charge in 24 hours, tolerance is with boiling-like.Cement pit is sterilized before putting in a suitable place to breed, 20cm of intaking is molten with 20PPm bleaching powder
Full pool spilling head after solution, after impregnating 48 hours, draining is rinsed well, is dried 3-4 days and is reused.When the standby parent selected puts in a suitable place to breed,
Using local fresh water, latter week being put in a suitable place to breed, when changing water every time, stepping up salinity, rise salinity 2-3 ‰ every time, salinity reaches to the end
To 12-15 ‰, rise salt and overwintering culture uses local salinity with water as ‰ estuarine waters of 10-15, the useful water of institute, which uses, to be passed through
Pond level-one precipitation, vinyl house cement pit nature sunlight pre-heating temperature elevation and two-stage precipitation, then filtered with 80 mesh sieve tulles, often
Zhou Huanshui 1 time, every time 2/3, every 25-35 days, sediment upturning 1 time, overwintering culture water temperature was maintained at 8 DEG C or more, changed water temperature difference less than 2
DEG C, sediment upturning water temperature difference is less than 1 DEG C；Bait is that commercial fresh-water fishes expanded pellet feed 3 is expected, crude protein content 38%, daily
It feeds 1 time, is subject to 1-2 hours and has ingested；Culture pond puts exopalaemon carinicauda in a suitable place to breed, and stocking rate is 5-10 jins/pond；
2nd, parent's spring reinforced cultivating：At the beginning of -4 months by the end of March, into parent's spring reinforced cultivating stage, plastics are big at this time
Water temperature has just started to reach 15-16 DEG C in canopy cement pit, with the rising of outside temperature, successively using the ventilation of vinyl house both sides,
It removes plastic film, capping first layer shade film, be capped second layer shade film again, keep 16-18 DEG C of water temperature in cement pit；Every time
When changing water, gradually reduce salinity, each salt reduction degree 2-3 ‰, to the end salinity reach local river water, salt reduction and reinforced cultivating water
Using local river water (fresh water), the useful water of institute using by pond level-one precipitation, shade greenhouse cement pit Temperature fall and
Two-stage precipitation, then filtered with 80 mesh sieve tulles, per changing within 5-6 days water 1 time, every time 2/3, every 2 weeks sediment upturnings 1 time, it is small to change water temperature difference
In 2 DEG C, sediment upturning water temperature difference is less than 1 DEG C；Bait is commercial No. 3 material of fish expanded pellet feed, crude protein content for 40% with
On, it feeds daily 2 times, is subject to 1-2 hours and has ingested；Other management are the same；
3rd, the parent that promotes production selects：At the beginning of -6 months mid-Mays, 17-19 DEG C of water temperature in the greenhouse culture pond that shades at this time, parent is female
Fish belly portion is expanded successively, can select promote production with parent, criteria for selection at this time：Raun postabdomen expands and soft, and cloacal aperture is micro-
Red, some gently press abdomen to have ovum outflow；Milter, it is light that cloacal aperture is pressed to have a small amount of sperm to flow out, and meet water and do not dissipate；The parent of select
Circuit of promoting production is put into using drum (100L) transport, every barrel puts 2-3 tail parents；Entire select will be softly with operation in transportational process
Not from water；
4th, parent promotes production：Circuit (referring to Fig. 1) promote production using rectangle cement pit (30.0m × 5.0m × 1.2m) 1, the depth of water
1.0m by constructing intermediate isolating wall 2 and smearing round pond corner, forms ellipse and is closed circuit (60.0m × 2.4m × 1.1m)
3, oxygenation is carried out using 0.75KW waterwheel aerators 4 and forms flow 5, is passed through using 10KW air source heat pump water heaters group 6
Cold and hot heat-dissipating pipe 7 keeps water temperature stability in circuit, and bow-shaped ring top is set above cement pit, and top covers three layers of shading rate
90% shade film ensures the intensity of illumination on daytime less than 300Lux, and parent's breeding density of promoting production is 0.8~1 tail/m2, male and female
Than being 1:1.1~1:1.3, water temperature of promoting production is 17.5~19 DEG C；
5th, natural spawning fertilization：After parent enters circuit of promoting production, to keep absolutely quiet around, using natural spawning fertilization
Mode remains closed circuit water velocity as 0.07~0.11m/s, and water temperature of promoting production is 17.5~19 DEG C；It is fed in promoting production pond few
Expanding compound feed is measured, to remove residual bait in time；
6th, fertilization ovum collecting：America hilsa herring parent lays eggs mating time in the morning at day, generally 5:00-6:00, it collects
Fertilized eggs general 7:00 starts, and before lower water is collected, suspends waterwheel aerator, after flow rate of water flow is allowed to lower, with the soft sieve of 24 mesh
Thin,tough silk triangle diddle-net is flat to copy bottom of pond at circuit dead angle, collects the operation of chalaza water, after copying ovum every time, is then turned on aerator 3-5 minutes, allows
Then ovum automatic turning copies ovum again at circuit dead angle, after being repeated 3 times, can then more than 95% ovum collecting be cleared up, be counted
Hatchery is moved on to after number.
Above-mentioned specific embodiment is passed through to be tested in this institute Technique of Raising Seeedling centre base, in 2014-2015 America hilsa herring parent
It cultivates and promotes production, add up to obtain fertilized eggs 17.76 ten thousand, concrete condition is as follows：
On May 21st, 2014 is hastened parturition parent using hormone, obtains ovum 110,000 in May 22 and 23 days, and fertilized eggs 4.0 ten thousand are average
Rate of fertilization 36.4%.
In May, 2015 point, 2 crowdes of America hilsa herring parents that promote production, obtained ovum 56.32 ten thousand altogether, and fertilized eggs 13.76 ten thousand are average to be fertilized
Rate 24.4%.First, the parent that promotes production for selecting for 2 ages on May 18 is put into east 10#, female 64, hero 76, sex ration 1：1.2, production
Ovum fertilization situation is shown in Table 1；Second batch, the parent that promotes production for selecting for 3 ages on May 28 are put into east 9#, female 71, hero 76, sex ration 1：
1.1, spawning and fertilization situation is shown in Table 2.
1 2015 years first parent's spawning and fertilization situation table of promoting production (east 10 of table#)
2 2015 years first parent's spawning and fertilization situation table of promoting production (east 9 of table#)
Claims (1)
1. a kind of method for obtaining batch America hilsa herring fertilized eggs, by standby parent overwintering culture, parent's spring reinforced cultivating, promotes production
Parent selects, parent promotes production, natural spawning fertilization, fertilization ovum collecting production stage composition, it is characterized in that 2 ages by artificial breeding
Standby parent of the more than America hilsa herring adult fish as artificial propagation selects healthy and strong, build grossness, weight in the annual 11-12 months
The commodity adult fish of 0.75-1.5Kg enters standby parent's overwintering culture, cultivates standby parent using vinyl house cement pit, uses
Pool wall is smooth, area 150-250m2Rectangular or round cement pit, deep 1.2-1.8m, cement pit top setting bow-shaped ring top, top
Portion covers transparent heat insulating plastic film, and with the shade membrane cover of shading rate 90% on a plastic film, light regulating reduces light transmission
Rate, while according to the hiding rare of water water colour adjusting shade film, it is typically maintained in 30%-40%；Gas head, gas are placed in cement pit
Head density is 0.2-0.3/m2, with Roots blower continuous charge in 24 hours, it is inflated to boiling-like；Disappear before putting in a suitable place to breed to cement pit
Poison, 20cm of intaking, full pool spilling head after being dissolved with 20PPm bleaching powder, after impregnating 48 hours, draining is rinsed well, dries 3-4 days again
It uses；When the standby parent selected puts in a suitable place to breed, using local fresh water, latter week is put in a suitable place to breed, when changing water every time, step up salinity, often
Secondary to rise salinity 2-3 ‰, salinity reaches 12-15 ‰ to the end, rises salt and overwintering culture uses local salinity with water as 10-15 ‰
Estuarine water, the useful water of institute by pond level-one precipitation, vinyl house cement pit nature sunlight pre-heating temperature elevation and two-stage precipitation,
It is filtered again with 80 mesh sieve tulles, changes water weekly 1 time, every time 2/3, every 25-35 days, sediment upturning 1 time, overwintering culture water temperature was maintained at
8 DEG C or more, water temperature difference is changed less than 2 DEG C, sediment upturning water temperature difference is less than 1 DEG C；Bait is commercial No. 3 material of fresh-water fishes expanded pellet feed,
Crude protein content is 38%, is fed daily 1 time, is subject to 1-2 hours and has ingested；In order to alleviate cement pit bottom excreta and residual bait
Deposition is corrupt and pollutes water quality, puts exopalaemon carinicauda in a suitable place to breed in cement pit, stocking rate is 5-10 jins/pond；At the beginning of -4 months by the end of March, enter
In parent's spring reinforced cultivating stage, water temperature has just started to reach 15-16 DEG C in vinyl house cement pit at this time, with outside temperature
Rise, using the ventilation of vinyl house both sides, dismounting plastic film, capping first layer shade film, be capped second layer shade film again, protect
16-18 DEG C of water temperature in water holding mud sump；When changing water every time, salinity is gradually reduced, each salt reduction degree 2-3 ‰ reaches local to the end
River water salinity, salt reduction and reinforced cultivating are with water using local river water, and the useful water of institute is by pond level-one precipitation, shade greenhouse
Cement pit Temperature fall and two-stage precipitation, then filtered with 80 mesh sieve tulles, per changing within 5-6 days water 1 time, every time 2/3, it was turned over every 2 weeks
Water temperature difference is changed less than 2 DEG C in pond 1 time, and sediment upturning water temperature difference is less than 1 DEG C；Bait is commercial No. 3 material of fish expanded pellet feed, slightly
Protein content is more than 40%, feeds daily 2 times, is subject to 1-2 hours and has ingested；Other management are the same；To mid-May-June
Just, 17-19 DEG C of water temperature in shade greenhouse cement pit, parent's raun abdomen expand successively at this time, can select promote production with parent at this time
This, criteria for selection：Raun postabdomen expands and soft, and cloacal aperture is micro- red, some gently press abdomen to have ovum outflow；Milter, light pressure are let out
Growing hole has a small amount of sperm to flow out, and meets water and do not dissipate；The parent of select is put into circuit of promoting production using the transport of 100L drums, and every barrel puts
2-3 tail parents；Entire select will be softly from water with operation in transportational process；Circuit of promoting production uses 30.0m × 5.0m × 1.2m
Rectangle cement pit, depth of water 1.0m by constructing intermediate isolating wall and smearing round pond corner, form ellipse and are closed circuit, adopt
Oxygenation is carried out with 0.75KW waterwheel aerators and forms flow, and cold and hot heat dissipation is passed through using 10KW air source heat pump water heaters group
Pipe keeps water temperature stability in circuit, and bow-shaped ring top is set above cement pit, and top covers the shade of three layers of shading rate 90%
Film ensures the intensity of illumination on daytime less than 300Lux, and parent's breeding density of promoting production is 0.8~1 tail/m2, sex ration 1:1.1~
1:1.3, water temperature of promoting production is 17.5~19 DEG C；Parent, which enters, to promote production after circuit, absolutely quiet around the circuit that keep promoting production, and uses
Natural spawning fertilization mode remains closed circuit water velocity as 0.07~0.11m/s, and water temperature of promoting production is 17.5~19 DEG C；Promote
A small amount of expanding compound feed is fed in production circuit, absorbs fertilized eggs, but to remove residual bait in time to reduce parent, otherwise puffing feeding
Material impregnates the time in water and has grown and will sink to the bottom, and is mixed into fertilized eggs, and fertilized eggs is caused to clean and detach impurity difficulty；America hilsa herring parent
Originally there is the custom for fertilized eggs of ingesting, fertilized eggs are collected in time after parent's oviposition, before collecting fertilized eggs, suspend Waterwheel-type oxygenation
After flow rate of water flow is allowed to lower, bottom of pond at circuit dead angle is copied with the soft bolting silk triangle diddle-net of 24 mesh is flat for machine, and America hilsa herring egg membrane is very thin,
Fertilized eggs cannot be squeezed from water, and collecting ovum will operate with water, after copying ovum every time, be then turned on aerator 3-5 minutes, allow ovum automatic turning
It moves at circuit dead angle, then copies ovum again, after being repeated 3 times, can then more than 95% ovum collecting be cleared up, be moved on to after counting
Hatchery.
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| CN105557601B (en) * | 2016-03-14 | 2017-12-19 | 上海市水产研究所 | A kind of Scatophagus argus (Linnaeus) survive the winter pattern foster with Macrobrachium rosenbergii set |
| CN105918165B (en) * | 2016-04-22 | 2019-07-30 | 苏州鱼之源生物科技有限公司 | A kind of aquaculture wintering method of the comprehensive current water of brackish water |
| KR102571026B1 (en) * | 2017-02-17 | 2023-08-28 | 가부시키가이샤 닛스이 | form system |
| CN107094667B (en) * | 2017-05-03 | 2019-10-29 | 上海市水产研究所 | A kind of America hilsa herring continental rise and Tu Chi are segmented relay-type cultural method |
| CN108552090B (en) * | 2018-05-09 | 2021-01-08 | 上海市水产研究所 | Greenhouse overwintering fattening method for second-instar alosa sapidissima migration-simulated soil pond |
| CN108617555B (en) * | 2018-05-09 | 2019-12-13 | 上海市水产研究所 | method for promoting mating and fertilization of alosa sapidissima parents by circulating water |
| CN110432192B (en) * | 2019-09-10 | 2021-07-16 | 上海市水产研究所（上海市水产技术推广站） | Method for prolonging artificial propagation time of alosa sapidissima |
| CN110663610B (en) * | 2019-10-30 | 2023-08-15 | 江苏省淡水水产研究所 | Industrial early propagation method and system for red swamp crayfish |
| CN112640834A (en) * | 2020-12-25 | 2021-04-13 | 嘉鱼县罗家桥养殖专业合作社 | Device and method for increasing yield of leiocassis longirostris fry |
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