CN110839572A - Artificial seedling culture method for babylonia areolata - Google Patents
Artificial seedling culture method for babylonia areolata Download PDFInfo
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- 241000920062 Babylonia areolata Species 0.000 title description 5
- 238000012136 culture method Methods 0.000 title description 2
- 241000237858 Gastropoda Species 0.000 claims abstract description 76
- 238000009395 breeding Methods 0.000 claims abstract description 43
- 230000001488 breeding effect Effects 0.000 claims abstract description 22
- 241000195493 Cryptophyta Species 0.000 claims abstract description 21
- 241000606434 Babylonia Species 0.000 claims abstract description 17
- 241000251468 Actinopterygii Species 0.000 claims description 49
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 48
- 238000012258 culturing Methods 0.000 claims description 22
- 239000004576 sand Substances 0.000 claims description 21
- 230000003203 everyday effect Effects 0.000 claims description 19
- 210000002700 urine Anatomy 0.000 claims description 19
- 241000700141 Rotifera Species 0.000 claims description 18
- 239000010813 municipal solid waste Substances 0.000 claims description 14
- 239000011259 mixed solution Substances 0.000 claims description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 10
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- 241000894006 Bacteria Species 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 241000238557 Decapoda Species 0.000 claims description 3
- 235000021552 granulated sugar Nutrition 0.000 claims description 2
- 241000913745 Spatholobus Species 0.000 claims 1
- 241000606431 Babylonia lutosa Species 0.000 abstract description 8
- 230000004083 survival effect Effects 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 4
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/51—Culture of aquatic animals of shellfish of gastropods, e.g. abalones or turban snails
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses an artificial breeding method of Babylonia, belonging to the technical field of aquaculture. The artificial breeding method of the babylonia lutosa, provided by the invention, provides the bait convenient for the larvae to eat, is simpler to operate, convenient to manage and time-saving compared with the daily feeding of unicellular algae, is not easy to cause death of the larvae, effectively improves the survival rate of the larvae growing into the larvae, ensures that the snail shells are black and exhausted when the larvae grow into the larvae, is full and hard in flesh, has a morbidity rate in the adult snail breeding stage far lower than that of adult snails bred by the traditional artificial breeding method, improves the yield of the babylonia lutosa into snails, and improves the economic benefit.
Description
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to an artificial breeding method of Babylonia.
Background
Babylonia lutosa (Babylonia lutosa) belongs to the phylum mollusca, the class of gastropoda, the order of Aphyllophorales, the family of Dryopteraceae, the genus Babylonia, is distributed in the east and south China sea, inhabits the sea area with mud under the tide, and lives in the camp and benthic. The mud dongfeng snail shell is thick and solid, the spiral part of the snail shell is in a cone shape, the body snail layer is expanded and is in a yellow brown color, the meat is delicious, the nutrition is rich, and the mud dongfeng snail shell is a shellfish with higher edible value. However, due to over-fishing before the 90 s and the increase of market demand, the wild babylonia areolata cannot meet the supply of the market, and the artificial propagation and seedling culture of the babylonia areolata are particularly important.
The conventional seedling raising method comprises the following steps: feeding marine fishes or crabs with aerated running water to breed parent bivalves, collecting oocysts, placing the oocysts in a mesh bag, aerating and culturing in running water, after larvae are hatched, feeding unicellular algae such as chrysophyceae, tetraselmis, chlorella and the like, changing water 1/4-1/3 every day, culturing to a young snail stage, starting benthic life, transferring young snails to a pool paved with 3-4cm fine sand, feeding minced fillet, aerating and culturing in running water for 1-2 months, transferring to a culture pool, feeding fresh cut marine fishes or crabs, keeping the water exchange amount 1-2 times every day, culturing to 10 g/crab, and collecting after reaching the commodity specification.
The existing artificial seedling raising method generally has the following problems: firstly, the problem of poor individual activity is easy to occur after the larvae of the eggs laid by the breeding snails emerge from membranes; secondly, the single-cell algae is fed in the planktonic stage of the larva, but the single-cell algae is divided into an initial stage, a vigorous stage, a decline stage and an aging stage, in the decline aging stage, because cell shells are hard and are not easy to digest, a large amount of death can occur after the larva swallows the single-cell algae, and the seedlings are difficult to eat, so that the larva sinks to the bottom, the shells are thin, the survival rate is low, diseases are more in the later stage, and the seedling cultivation amount and yield are seriously influenced.
Disclosure of Invention
In order to overcome the defects of the prior art, the technical problems to be solved by the invention are as follows: how to improve the problems of poor larva constitution in the artificial fry process of the babylonia lutosa and low bottom sinking, thin shell and low survival rate in the larva cultivation process.
In order to solve the technical problems, the invention adopts the technical scheme that: artificial seedling growing method for babylonia lutosa
The method comprises the following steps:
step 1, uniformly stirring small trash fish, brown sugar and distiller's yeast in a mass ratio of 10:1:0.1, sealing and fermenting for 25-35h, uniformly stirring, and filtering with a 40-mesh nylon net to obtain fermented fish paste; boiling human urine for 20min to obtain treated human urine; uniformly mixing the fermented fish paste and the treated human urine in a plastic barrel according to the mass ratio of 2:1 to obtain a fish paste mixed solution;
step 2, filling unfiltered seawater into the larva cultivating pool, inflating, applying 10ppm of fish pulp mixed liquor, oxygenating for 3d, placing the newly hatched larva after observing that the water color and the density of microorganisms such as rotifers and the like are proper, and applying 3-5ppm of fish pulp mixed liquor every day when the densities of the rotifers and the algae in the cultivating pool are insufficient until young snails are cultivated;
and 3, paving fine sand at the bottom of the young snail cultivation pool, uniformly scattering the young snails into the sand, inflating and adding convection water, changing water every day and feeding fresh oyster slurry once, after cultivation for 18-22d, grading the young snails by using a 40-mesh net bag, continuously cultivating the young snails outside the net bag in the young snail cultivation pool, and transferring the young snails in the net bag into the cultivation pool for cultivation.
The invention has the beneficial effects that: according to the artificial breeding method of the babylonia areolata, the fermented fish paste is prepared from the small trash fish, the brown sugar and the distiller's yeast, and the processed human urine is matched to culture algae and rotifers in natural seawater to serve as the initial feed of larvae, so that the larvae can conveniently eat more abundant and balanced nutrition.
Detailed Description
In order to explain the technical content, the objects and the effects of the present invention in detail, the following description will be given with reference to the embodiments.
The most key concept of the invention is as follows: the algae and rotifer in natural seawater are cultured by using fermented fish paste and treated human urine as initial feed for larvae.
The invention provides an artificial breeding method of Babylonia, which comprises the following steps:
step 1, seedling preparation: uniformly stirring small trash fish, brown sugar and distiller's yeast in a mass ratio of 10:1:0.1, sealing and fermenting for 25-35h, uniformly stirring, and filtering with a 40-mesh nylon net to obtain fermented fish paste; boiling human urine for 20min to obtain treated human urine; mixing the fermented fish paste and the treated human urine in a plastic barrel according to the mass ratio of 2:1 to obtain a fish paste mixed solution;
step 2, larva cultivation: filling unfiltered seawater into the larva cultivating pool, inflating, applying 10ppm fish pulp mixed solution, oxygenating for 3d, placing the larva which is just hatched after observing that the density of microorganisms such as water color and rotifer is proper, and applying 3-5ppm fish pulp mixed solution every day when the density of rotifer and algae in the cultivating pool is insufficient until young snails are cultivated;
step 3, culturing young snails: spreading fine sand (with fineness modulus of 2.2-1.6) at the bottom of the young snail culture pond, uniformly scattering the young snails into the sand, inflating and adding convection water, changing water every day and feeding fresh oyster slurry once, culturing for 18-22d, then grading the young snails by using a 40-mesh bag, continuously culturing the young snails outside the mesh bag in the young snail culture pond, and transferring the young snails in the mesh bag to the culture pond for culturing.
From the above description, the beneficial effects of the present invention are: the artificial breeding method of the Babylonia, provided by the invention, adopts the small trash fish, the brown sugar and the distiller's yeast to prepare the fermented fish paste, and then the algae and the rotifer in the natural seawater are cultured by matching with the treated human urine to serve as the initial feed of the larvae, so that the larvae can conveniently eat more abundant and balanced feed, compared with the daily feeding of unicellular algae, the larvae are not easy to die, the oyster paste is fed during the breeding of the larvae, compared with the feeding of wild trash fish in the prior art, the method has the advantages of strong palatability, high nutrition, easy absorption and digestion of the larvae, less residual water in a culture pond, difficult pollution, simpler operation, convenient management and time saving, through the artificial breeding method, the survival rate of the larvae growing into the larvae is effectively improved, and the black and exhausted color of the snail shells during the later growth into larvae, the flesh shells are hard, and the morbidity of the adult snail breeding stage is far lower than that obtained by the traditional artificial breeding method, the yield of the Babylonia into the snail is improved, and the economic benefit is improved.
Further, step 1 is preceded by a step of breeding the snails: laying medium coarse sand (fineness modulus is 3.0-2.3) with thickness of 10cm in a seedling culture pond, adding 50kg of individual giant healthy seed snails, adding aerated running water, feeding fresh frozen trash fish or water crab (crab which is just hulled or is about to be hulled) every day, changing water every day, stopping adding water and sprinkling white sugar when newly added water is 1/5 of the volume of the seedling culture pond, adding running water after 2h, and culturing until the seed snails mate to produce egg cell pieces, wherein the sprinkling amount of the white sugar is 2kg per 16m of water 3.
From the above description, in the breeding snail cultivation stage, the body constitution of the larva after membrane emergence can be improved by applying the white sugar in the water body every day, and the obtained larva is large, vigorous and healthy and active, and the volume of the larva is increased by about 1 time compared with the larva produced by the egg piece produced by the breeding snail which is not fed with the white sugar.
Furthermore, the seedling culture pond is a cement pond, and the size of the cement pond is 8m in length, 2m in width and 1m in height.
Further, when the fermented fish paste is prepared in the step 1, 1kg of brown sugar and 100g of distiller's yeast are added to 10kg of small trash fish.
Further, the size of the larva culturing pool in the step 2 is 8m in length, 2m in width and 1m in height, and the larva culturing time in the larva culturing pool is 12-20 days.
Further, the size of the bottom of the young snail culture pond is 8m in length, 2m in width and 1m in height, 10cm of fine sand is paved at the bottom of the young snail culture pond, and the number of the young snails in each pond is about 20 ten thousand.
Further, in the step 2, when the density of the rotifers and the algae in the larva cultivation pool is too high, 2ppm of EM bacteria are splashed into the larva cultivation pool.
According to the description, when the density of the rotifers and the algae is too high, the EM bacteria are added to effectively adjust the water quality, so that the water body deterioration caused by excessive reproduction of the rotifers and the algae, further death, influence on the water quality and influence on the growth of young snails are avoided. And (3) judging the density of the rotifers and the algae in the culture pond, specifically, according to the standard, taking 250ml of water in the culture pond by using a glass beaker, wherein the water color is dark green, then irradiating the dark place with a strong light lamp, and observing that more rotifers swim in the water, wherein the proper density is obtained at the moment.
Further, the step 3 is followed by a step of snail formation culture: and (3) transferring the young snails in the mesh bags classified in the step (3) to a breeding pond, feeding fresh fish paste for breeding, putting the breeding pond into the breeding pond, and paving coarse sand at the bottom of the pond, wherein the size of the coarse sand is 8m long by 2m wide by 1m high, and the thickness of the sand is 15-20 cm.
The first embodiment of the invention is as follows:
an artificial breeding method of Babylonia includes the following steps:
step 1, seedling preparation: uniformly stirring small trash fish, brown sugar and distiller's yeast in a mass ratio of 10:1:0.1, and sealing and fermenting for 25-35h to obtain fermented fish paste; boiling human urine for 20min to obtain treated human urine; uniformly mixing the fermented fish paste and the treated human urine in a plastic barrel according to the mass ratio of 2:1 to obtain a fish paste mixed solution;
step 2, larva cultivation: filling unfiltered seawater into the larva cultivating pool, inflating, applying 10ppm fish pulp mixed solution, oxygenating for 3d, placing the larva which is just hatched after observing that the density of microorganisms such as water color and rotifer is proper, and applying 3-5ppm fish pulp mixed solution every day when the density of rotifer and algae in the cultivating pool is insufficient until young snails are cultivated;
step 3, culturing young snails: spreading fine sand at the bottom of the young snail cultivation pool, uniformly scattering the young snails into the sand, adding water and inflating, changing water every day and feeding fresh oyster slurry once, after cultivating for 18-22d, using a 40-mesh bag to grade the young snails, continuously cultivating the young snails outside the mesh bag in the young snail cultivation pool, and transferring the large-size young snails into the cultivation pool for cultivation.
The second embodiment of the invention is as follows:
an artificial breeding method of Babylonia, which is characterized by comprising the following steps:
step 1, breeding snail: laying medium coarse sand (fineness modulus is 1.6-3.7) with thickness of 10cm at the bottom of a cement seedling culture pond with size of 8m x 2m x 1m, putting 50kg of individual giant healthy breeding snails, adding aerated running water, feeding fresh frozen trash fish or water crab (crab which is just hulled or is about to be hulled) every day, changing water every day, stopping adding water and sprinkling 2kg of white granulated sugar when newly added water is 1/5 of the volume of the seedling culture pond, adding running water (running water culture) after 2h, and culturing until the breeding snails mate to produce egg cell slices;
step 2, seedling preparation: mixing the components in a mass ratio of 10:1: uniformly stirring 0.01 small trash fish, brown sugar and distiller's yeast, sealing and fermenting for 30h, uniformly stirring, and filtering with 40-mesh nylon net to obtain fermented fish paste; boiling human urine for 20min to obtain treated human urine; uniformly mixing the fermented fish paste and the treated human urine in a plastic barrel according to the mass ratio of 2:1 to obtain a fish paste mixed solution;
step 3, larva cultivation: filling unfiltered seawater into an 8m 2m 1m larva cultivation pool, inflating, applying 10ppm fish pulp mixed solution, oxygenating for 3d, placing 100 million of oocytes after membrane removal, changing water amount to 1/6-1/7 of total water amount every day after observing that the density of microorganisms such as algae and rotifer in the larva cultivation pool is not enough, applying 3-5ppm fish pulp mixed solution every day; when the density of rotifers and algae in the larva cultivating pond is too high, 2ppm of EM bacteria are splashed into the larva cultivating pond, young snails are obtained after cultivation for 12-20 days, generally, the young snails climb the wall after about 12 days, floating larvae appear after 18-20 days, and the young snails are collected by a 120-mesh nylon mesh bag;
step 4, culturing young snails: laying 10cm of fine sand at the bottom of a young snail cultivation pool of 8m x 2m x 1m, uniformly spraying the young snails into the sand, wherein the number of the young snails in each pool is about 20 thousands, inflating and adding convection water, changing water every day and feeding fresh oyster slurry once, after cultivating for 18-22d, grading the young snails by using a 40-mesh bag, continuously cultivating the young snails outside the mesh bag in the young snail cultivation pool, and transferring the young snails in the mesh bag into a cultivation pool for cultivation;
step 5, adult snail cultivation: transferring the young snails in the classified mesh bag into a breeding pond, and feeding fresh fish paste for breeding. Placing the culture pond with size of 8m 2m 1m, and spreading coarse sand at the bottom of the pond, wherein the thickness of the sand is 15-20 cm.
In conclusion, the artificial breeding method for babylonia lutosa provided by the invention adopts small trash fish, brown sugar and distiller's yeast to prepare fermented fish paste, and the algae and rotifer in natural seawater are cultured by matching with the treated human urine to serve as the initial feed for larvae, so that the larvae can conveniently eat more nutritious and balanced feed, compared with the daily feeding of unicellular algae, the larvae are not easy to die, the operation is simpler, the management is convenient and the time is saved, by the artificial breeding method, the survival rate of the larvae growing into the larvae is effectively improved, the shells of the larvae growing into the larvae are black and exhausted when the larvae grow into the larvae, the bodies are full and the shells are hard, the incidence rate in the adult breeding stage is far lower than that of adult snails cultured by the traditional artificial breeding method, the yield of the babylonia lutosa adults is improved, and the economic benefit is improved.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent modifications made by the present invention in the specification or directly or indirectly applied to the related technical field are included in the scope of the present invention.
Claims (8)
1. The artificial breeding method of Babylonia is characterized by comprising the following steps:
step 1, uniformly stirring small trash fish, brown sugar and distiller's yeast in a mass ratio of 10:1:0.1, sealing and fermenting for 25-35h, uniformly stirring, and filtering with a 40-mesh nylon net to obtain fermented fish paste; boiling human urine for 20min to obtain treated human urine; uniformly mixing the fermented fish paste and the treated human urine according to the mass ratio of 2:1 to obtain a fish paste mixed solution;
step 2, filling unfiltered seawater into the larva culturing pool, inflating, applying 10ppm fish paste mixed solution, oxygenating for 3 days, and placing the newly hatched larva until young snails are cultured;
and 3, paving fine sand at the bottom of the young snail cultivation pool, uniformly scattering the young snails into the sand, inflating and adding convection water, changing water every day and feeding fresh oyster slurry once, after cultivation for 18-22d, grading the young snails by using a 40-mesh net bag, continuously cultivating the young snails outside the net bag in the young snail cultivation pool, and transferring the young snails in the net bag into the cultivation pool for cultivation.
2. The artificial breeding method of Babylonia mudagrica according to claim 1, characterized by further comprising the step of breeding Babylonia mudagrica before the step 1: laying medium coarse sand with the thickness of 10cm in a seedling culture pond, putting 50kg of breeding snails, adding aerated flowing water, feeding fresh frozen trash fish or water crabs every day, changing water every day, stopping adding water and sprinkling white granulated sugar when newly added water is 1/5 of the volume of the seedling culture pond, adding counter flowing water after 2h, and culturing until the breeding snails mate to produce egg cell pieces.
3. The artificial breeding method of bulleyaconitum amazonicum according to claim 2, wherein the size of the seedling culture pond is 8m by 2m by 1 m.
4. The artificial breeding method of Babylonia according to claim 1, wherein 1kg of brown sugar and 100g of distiller's yeast are added to 10kg of small trash fish when preparing the fermented fish paste in step 1.
5. The method for artificially culturing spatholobus bullosa as claimed in claim 1, wherein the size of the larva culturing pool in step 2 is 8m x 2m x 1m, and the larva culturing time in the larva culturing pool is 12-20 days.
6. The artificial breeding method of Babylonia mudagrass as claimed in claim 1, wherein the size of the bottom of the young Babylonia mudagrass culture pond is 8m x 2m x 1m, and 10cm of fine sand is paved at the bottom of the young Babylonia mudagrass culture pond.
7. The artificial breeding method of bulleyaconitum amansii according to claim 1, wherein 2ppm of EM bacteria is sprinkled into the larva cultivating pond when the density of rotifer and algae in the larva cultivating pond is too high in step 2.
8. The artificial breeding method of Babylonia mudagrass as claimed in claim 1, further comprising the step of culturing Babylonia mudagrass after the step 3: transferring the young snails in the mesh bag after grading in the step 3 to a breeding pond, and feeding fresh fish paste for breeding.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911278733.0A CN110839572A (en) | 2019-12-13 | 2019-12-13 | Artificial seedling culture method for babylonia areolata |
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