CN117617162B - Interspecific hybridization method of Babylonia pusilla and Babylonia quadricarina - Google Patents
Interspecific hybridization method of Babylonia pusilla and Babylonia quadricarina Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 42
- 238000009403 interspecific hybridization Methods 0.000 title claims abstract description 40
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- 230000001488 breeding effect Effects 0.000 claims abstract description 32
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- 210000003250 oocyst Anatomy 0.000 claims abstract description 15
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- 238000009396 hybridization Methods 0.000 claims abstract description 8
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to a method for interspecific hybridization of Babylonia tussilago and Babylonia quadricarina. Firstly, utilizing mud Babylonia and Babylonia groups of multiple generations to continuously select and breed interspecific hybridization to establish multiple hybrid first-generation families, selecting excellent hybrid families according to the phenotype of each hybrid family, then mixing and breeding groups in the excellent hybrid families, hatching oocysts to obtain hybrid second-generation offspring groups, breeding the offspring to obtain the finished product of hybrid second-generation Babylonia, utilizing green light or blue light and semen astragali complanati to promote gonad maturation of the snails, shortening gonad development time, then carrying out mixed hybridization and propagation in preferred families, and continuously selecting and breeding multiple generations according to the same method to obtain new Babylonia hybrid variety with stable hereditary property and excellent property. The new hybrid variety of Babylonia obtained by the method has the advantages of strong disease resistance, stable heredity, fast growth, large individual, high meat yield, and excellent characteristics of Babylonia pusilla and Babylonia quadricarina.
Description
Technical Field
The invention belongs to the technical field of shellfish breeding, and particularly relates to an interspecific hybridization method of Babylonia tussilago and Babylonia quadricarina.
Background
Babylonia (Babylonia areolata) is commonly called as a conch, babylonia (lumosa) is commonly called as a yellow conch, both belong to the phylum Mollusca (Mollusca), gastropoda (Gastropoda), front gill subclass (Prosobranchia), new Abdominal order (Neogastropoda), babyloniaceae (Babyloniaidae) and Babylonia (Babylonia), are main breeding varieties of Babylonia in China, are mainly distributed in tropical and subtropical areas, are predatory shallow seafloor shellfish, are delicious in taste, and are high-protein and low-fat famous seafood meeting modern nutritional requirements.
The Babylonia is an important seawater economic shellfish cultivated in the south of China, the cultivation scale and cultivation yield of the Babylonia are continuously increased in the last 20 years, and the annual yield of only two provinces of Hainan and Fujian at present reaches 2 tens of thousands of tons; during the period, scholars also do germ plasm improvement, such as group breeding (new variety "Haitai Yi"). The patent CN 104798709A discloses a breeding method of the hybrid seed of the Babylonia quadricarina, which adopts the processes of male parent of the Babylonia quadricarina in Thailand female parent of the Babylonia quadricarina, female parent of the Babylonia quadricarina and male parent of the Babylonia quadricarina in Thailand, and the female parent of the Babylonia quadricarina is hybridized, the female parent of the Babylonia is judged by sex, the female parent of different geographical populations is hybridized, the nutrition strengthening cultivation of the female parent is carried out, the oocyst collection and incubation, the larva cultivation, the young snail cultivation and the like, and the hybrid seed with high growth speed and strong disease resistance is cultivated.
However, in recent years, the screw diseases have comprehensive, complex and frequent characters, are more difficult to control, and the outbreak diseases in the breeding process are frequently seen, so that the improved germplasm is difficult to deal with. Therefore, on the basis of biological research of the Babylonia, the breeding of the Babylonia with strong disease resistance, strong stress resistance, stable heredity, fast growth, large individual, high meat yield and high yield through interspecific hybridization becomes a technical problem to be solved urgently, and is also important for sustainable healthy development of the Babylonia breeding industry.
Disclosure of Invention
Aiming at the prior art problems, the primary aim of the invention is to provide an interspecific hybridization method of Babylonia pusilla and Babylonia quadricarina, and the new variety of Babylonia hybrid cultivated by the interspecific hybridization method has the advantages of strong disease resistance, strong stress resistance, stable heredity, fast growth, large individual, high yield and high yield. Compared with the existing Babylonia tussilaga and Babylonia quadricarina, the survival rate, the yield and the long speed of the new hybrid variety of the Babylonia provided by the invention are obviously improved.
The second purpose of the invention is to provide the hybrid Babylonia with strong disease resistance and long speed obtained by the interspecific hybridization method of the Babylonia with the Babylonia quadricarina.
The third object of the invention is to provide the application of the interspecific hybridization method of the mud Babylonia and the Babylonia quadricarina in cultivating the hybrid Babylonia with strong disease resistance and long speed.
In order to achieve the above object, the present invention is realized by the following technical scheme:
An interspecific hybridization method of Babylonia tussilago and Babylonia quadricarina comprises the following steps:
s1, continuously breeding an intraspecific mixed family of Babylonia tussilago and Babylonia quadricarina, and taking the Babylonia tussilago as a seed;
s2, performing gonad ripening treatment and interspecific hybridization pairing on the seed snails obtained in the step S1 to obtain interspecific hybridization paired oocysts;
S3, carrying out scale breeding on oocysts obtained by the inter-seed hybridization pairing in the step S2 to obtain inter-seed first-filial generation offspring seeds, and culturing the inter-seed first-filial generation offspring seed populations until finished products are obtained to obtain the first-filial generation Babylonia;
S4, culturing the first-generation Babylonia of the hybrid in the step S3 until gonad is mature, inducing spawning, carrying out population mixing in the family to obtain oocysts, and incubating the oocysts to obtain a second-generation hybrid seed population; culturing the hybrid second-generation seedling population to a finished product, and taking the weight and the shell length as screening standards, wherein the screened family is the hybrid second-generation Babylonia;
S5, carrying out population mixing propagation on the second generation Babylonia of the hybrid in the family, and continuously breeding for multiple generations to obtain the hybrid Babylonia with strong disease resistance and long speed.
The invention carries out continuous breeding on the intraspecific mixed families of the Babylonia tussilago and the Babylonia quadricarina, and the continuous breeding of the seeds finds out that the individuals with excellent properties of the Babylonia tussilago and the Babylonia quadricarina are recombined, thereby being more beneficial to the subsequent interspecific hybridization to generate advantages and inheritance. The invention then carries out gonad ripening treatment on the mud snails and the Babylonia quadricarina which are continuously bred for multiple generations, shortens the gonad development time, adopts mud snails and Babylonia quadricarina groups to carry out intersystem hybridization to establish a plurality of hybrid first-generation families, selecting an excellent hybrid family according to the phenotype of each hybrid family, mixing the groups in the excellent hybrid family, incubating oocysts to obtain a hybrid second-generation offspring seed group, and culturing the offspring seed to a finished product to obtain the hybrid second-generation Babylonia. And (3) carrying out population mixed propagation on the second-generation Babylonia hybrida in the family, and continuously breeding for multiple generations according to the same method to obtain the hybrid Babylonia with strong disease resistance and high speed. The new Babylonia hybrid variety obtained by the interspecific hybridization method has the advantages of strong disease resistance, strong stress resistance, stable heredity, quick growth, large individual, high yield and high yield.
Compared with Babylonia tussilago, babylonia tussilago has the characteristics of high speed, but poor disease resistance (low survival rate); compared with Babylonia quadricarina, the Babylonia quadricarina has the characteristics of strong disease resistance and low survival rate. The inventor surprisingly found that, compared with the original and existing mud Babylonia and Babylonia with square spots, the survival rate of the new hybrid variety of the Babylonia obtained by the invention is obviously better than that of Fang Ban Babylonia and Babylonia, and the long speed of the new hybrid variety of the Babylonia is also obviously better than that of Babylonia with square spots; in addition, during the more brief cultivation period, the yield of the new cross varieties of the Babylonia is also significantly higher than Fang Ban Babylonia and Babylonia. The invention utilizes hybridization cultivation to form new variety of Babylonia, enriches the variety of Babylonia, ensures the health, green and sustainable development of Babylonia industry, and promotes the transformation of Babylonia industry from yield scale benefit type to yield, quality and environment comprehensive benefit type through the creation of improved variety. The interspecific hybridization method provided by the invention can be applied to the culture modes of indoor small water body culture, beach culture, outdoor sowing and the like.
Preferably, in the step S1, the continuous breeding is to select 2 or more generations of intraspecific hybrid families.
Preferably, in the step S1, the breeding is to take weight, shell length and survival rate as target traits, and cut off and leave the offspring of the intraspecific hybrid with 10% of selection pressure.
Preferably, in the step S1, the individual shells of the seed snails have a length of more than 30mm, a weight of more than 12g, and complete and intact shells and good vitality.
Preferably, in the step S2, the interspecific hybridization pairing is a single pair hybridization. Further preferably, during the interspecific hybridization pairing, the male and female pairing is performed according to 1:1, the weight ratio of male to female is 1: 1-2, constructing a plurality of families.
Preferably, in the step S2, the gonadal maturation process is: the green light or blue light is set in the external environment, and the light intensity is controlled to be 16-32 mu mol.m -2·s-1. The invention promotes the gonad development of the Babylonia by building a specific light environment, can lead the gonad maturity of the Babylonia and the Babylonia with the square spots to be synchronous, and shortens the gonad development time until the gonad development is mature. Further preferably, the photoperiod is 12h light and 12h dark during gonadal maturation treatment.
Preferably, in the step S3, the conditions for the scale-up breeding are: the illumination is 500-1000 lx, the salinity is 20-34, and the water temperature is 22-31 ℃.
Further preferably, in the large-scale breeding process, the larvae feed the golden algae in the early stage, the flat algae and the diatom are added in the middle morning, the shrimp slices, the shrimp elements, the spirulina powder, the vitamins and other artificial baits are fed in the evening, and the microcapsules are fed in the later stage.
Preferably, in the step S4, during the process from the cultivation of the first filial generation of Babylonia to gonad maturation, the fed baits are mixed with the semen astragali complanati extract.
Preferably, the dosage of the semen astragali complanati extract accounts for 0.1% -0.3% of the weight of the bait.
Preferably, the preparation method of the semen astragali complanati extract comprises the following steps: reflux extracting semen astragali Complanati with ethanol solution under heating, vacuum filtering, concentrating under reduced pressure to obtain extract, and drying to obtain semen astragali Complanati extract.
Preferably, the alcoholic solution may be an alcoholic solution conventionally used in the art for plant extraction. More specifically, ethanol and the like.
Preferably, the heating temperature is 50 to 100 ℃.
Preferably, the concentration of the ethanol is 40-80%.
Preferably, the times of reflux extraction are 2-3 times, and leaching liquor obtained by the reflux extraction for a plurality of times is combined and then is subjected to suction filtration.
Preferably, the drying is spray drying, and the temperature of the spray drying is 50-80 ℃.
Preferably, in the step S5, the breeding is continuously performed for more than 3 generations.
Furthermore, the invention also claims to protect the hybrid Babylonia which is obtained by the interspecific hybridization method of the Babylonia pusilla and Babylonia quadricarina and has strong disease resistance and long and fast speed.
Furthermore, the invention also claims the application of the interspecific hybridization method of the mud Babylonia and the Babylonia quadricarina in the cultivation of the hybrid Babylonia with strong disease resistance and long speed.
Compared with the prior art, the invention has the following beneficial effects: the invention provides an interspecific hybridization method of Babylonia pusilla and Babylonia quadricarina, and the new hybrid variety of Babylonia obtained by the method has the advantages of strong disease resistance, stable heredity, fast growth, large individual and high meat yield, and simultaneously has the excellent characteristics of Babylonia pusilla and Babylonia quadricarina. The invention utilizes hybridization cultivation to form new variety of Babylonia, enriches the variety of Babylonia, ensures the health, green and sustainable development of Babylonia industry, and promotes the transformation of Babylonia industry from yield scale benefit type to yield, quality and environment comprehensive benefit type through the creation of improved variety.
Drawings
FIG. 1 is a flow chart of a method for cultivating a new hybrid variety of Babylonia.
Detailed Description
The invention is further illustrated in the following drawings and specific examples, which are not intended to limit the invention in any way. Unless specifically stated otherwise, the reagents, methods and apparatus employed in the present invention are those conventional in the art.
EXAMPLE 1 method for interspecific hybridization of Babylonia in mud and Babylonia quadricarina
(1) Taking the weight, the shell length and the survival rate as target characters, intercepting and retaining the offspring of the intraspecies hybrid families of the Zhanjiang river Babylonia and the southwestern sea Babylonia with 10% of selection pressure, and continuously breeding for 2 generations to serve as seed snails; wherein, the seed snail is selected by the length of a single individual shell of more than 30mm, the weight of more than 12g, and the shell is complete and has no damage and good vitality.
(2) The seed snails obtained in the step (1) are placed in an external environment to be set with green light, the light intensity is controlled to be 16-32 mu mol.m -2·s-1, the light period is a culture pond with 12h illumination and 12h darkness for culturing, and when the gonad maturity of the mud Babylonia and the Babylonia are synchronous and the gonad development is mature, the seed snail is subjected to interspecific hybridization pairing, and in the interspecific hybridization pairing process, in order to avoid the influence of individuals with sexual aberration, the male and female pairing is carried out according to the following formula 1:1, the weight ratio of male to female is 1:1, constructing a plurality of families to obtain the oocysts matched with the interspecific hybridization.
(3) The oocysts obtained by the interspecific hybridization pairing in the step (2) are subjected to scale propagation under the conditions of 500-1000 lx of illumination, 20-34 of salinity and 22-31 ℃ of water temperature, in the scale propagation process, the golden algae is fed to the larvae in the early stage, the flat algae and the diatoms are fed in the middle morning, the baits such as shrimp slices, the shrimp elements, the spirulina powder and the vitamins are fed to the larvae in the evening, the microcapsules (the opening baits A0 of the Europe biological sciences Co., the east pill Miao Bao) are fed for 2-3 times a day, the feeding amount of the microalgae is 3-8 tens of thousands/ml each time, and the microalgae color of the water body is always kept. And breeding offspring in a large scale to obtain interspecific hybrid first-generation offspring, and breeding interspecific hybrid first-generation offspring population until the finished product is obtained to obtain the hybrid first-generation Babylonia.
(4) And (3) cultivating the first-filial generation Babylonia to gonad maturity, wherein during the maturation promoting period, the bait is mixed with semen astragali complanati extract, the dosage of the semen astragali complanati extract accounts for 0.1-0.3% of the weight ratio of the bait, the spawning is induced, the population in the family is mixed to obtain oocysts, the oocysts are hatched to obtain a second-filial generation seed population, the second-filial generation seed population is cultivated in a spiral pool until the finished product, and the specification (length, width, and height) of the spiral pool is 6m multiplied by 2.5m multiplied by 0.5m. A plastic partition board is paved in the pond, an 80-mesh bolting silk net is paved on the upper layer of the partition board, and the periphery is sealed. And (3) spreading sand on the bolting silk net, wherein the grain diameter of the sand is 2-2.5 mm. A black sunshade net with 80 meshes is hung around and on the top of the spiral pool field. The water temperature is 20-31 ℃ during the cultivation period, feeding is carried out once in two days, the feeding is carried out at the evening, the feeding amount is 5-10% of the total weight of the snails, the baits are trash fish, crabs, oysters and squids, and the water flowing state of the snail pond is kept for 24 hours; and (5) taking the weight and the shell length as the standards, screening out the families with excellent character performance and top 10 percent of rank, namely the second generation Babylonia as the hybrid.
(5) And (4) carrying out population mixing propagation in families on the second-generation Babylonia of the hybrid, and continuously breeding for three generations to obtain a new Babylonia hybrid variety with stable heredity and excellent properties (strong disease resistance and high speed).
Example 2 cultivation of New Babylonia hybrid variety
In the culture of Wenchang in Hainan, the specification (length, width and height) of the culture pond is 6m, 2.5m and 0.5m. A plastic partition plate is paved in the pond, an 80-mesh bolting silk net is paved on the upper layer of the partition plate, and the periphery of the partition plate is sealed to prevent sand leakage. Spreading sand on the bolting silk net, wherein the grain diameter of the sand is 1.5-2.5 mm, the thickness of the sand layer is 1.5-3.0 cm, disinfecting the sand after spreading sand, flushing with water until no muddiness is seen, soaking and disinfecting with potassium permanganate with the concentration of 0.5% for 4 hours, flushing with water after disinfecting, then sprinkling oxalic acid solution to dissolve out residual potassium permanganate attached to the pool wall and the sand, and flushing with water again to complete the disinfection of the sand layer and the spiral pool. The periphery and the top of the spiral pool field are respectively hung with a black sunshade net with 80 meshes. The new hybrid young snail of Babylonia cultivated in the example 1 is put into a pool for cultivation, the shell length of the young snail is 0.5 cm-1.0 cm, and the stocking density of the young snail is 1200 grains/m 2. Keeping running water continuously for 24 hours, and stopping water when feeding and collecting residual baits. The fish feed is fed once every two days, and the mixed fish is mainly used, so that the oysters and squids are properly replaced. The daily feeding amount is 5% -15% of the total weight of the snails in the pool, and the feeding amount is increased along with the growth of the snails. The feeding time is 17 to 20 pm.
Results of cultivation: after 198 days of cultivation, 365 jin of commodity snails are harvested, 45-50 snails per jin, and the survival rate is 95.2%.
Example 3 cultivation of novel Babylonia hybrid variety
The specific cultivation method of this embodiment is shown in embodiment 2.
Results of cultivation: after 198 days of cultivation, 354 jin of commodity snails are harvested, 45-50 snails per jin, and the survival rate is 94.6%.
Comparative example 1 cultivation of Babylonia
This comparative example differs from example 2 in that: the offspring was the Babylonia Zhanjiang mud in example 1.
Results of cultivation: after 282 days of cultivation, 303 jin of commodity snails are harvested, 50-55 snails per jin, and the survival rate is 90.5%.
Comparative example 2 cultivation of Babylonia
This comparative example differs from example 2 in that: the offspring was the Babylonia Zhanjiang mud in example 1.
Results of cultivation: after 275 days of cultivation, 310 jin of commodity snails are harvested, 50-55 snails per jin, and the survival rate is 91.4%.
Comparative example 3 cultivation of Babylonia quadricarina
This comparative example differs from example 2 in that: the offspring was Babylonia hainanensis of example 1.
Results of cultivation: after 214 days of cultivation, 302 jin of commodity snails are harvested, 45-55 snails per jin, and the survival rate is 81.7%.
Comparative example 4 cultivation of Babylonia quadricarina
This comparative example differs from example 2 in that: the offspring was Babylonia hainanensis of example 1.
Results of cultivation: after 222 days of cultivation, 310 jin of commodity snails are harvested, 45-55 snails per jin, and the survival rate is 83.6%.
As can be seen from example 2, comparative example 1 and comparative example 2, the new species of Babylonia obtained by the interspecific hybridization method of Babylonia in mud and Babylonia in the invention are cultivated, and the commodity snails which can be harvested for 365 jin only need 198 days for cultivation, while comparative example 1 and comparative example 2 adopt the Babylonia for cultivation, the cultivation time is far longer than that of the new species of Babylonia (282 days and 275 days respectively), and the survival rate of the Babylonia in mud is lower than that of the new species of Babylonia in the invention.
As can be seen from example 2, comparative example 3 and comparative example 4, the survival rate of the new species of the Babylonia obtained by the interspecific hybridization method of the Babylonia in mud and Babylonia in the invention can reach 95.2%, while the cultivation time of the Babylonia in comparative example 1 and comparative example 2 is longer than that of the new species of the Babylonia in the invention, and the survival rate of the Babylonia in square is obviously lower than that of the new species of the Babylonia in the invention.
The foregoing examples are illustrative only and serve to explain some features of the method of the invention. The claims that follow are intended to claim the broadest possible scope as conceivable and the embodiments presented herein are demonstrated for the applicant's true test results. It is, therefore, not the intention of the applicant that the appended claims be limited by the choice of examples illustrating the features of the invention. Some numerical ranges used in the claims also include sub-ranges within which variations in these ranges should also be construed as being covered by the appended claims where possible.
Claims (10)
1. The interspecific hybridization method of the Babylonia tussilago and the Babylonia quadricarina is characterized by comprising the following steps of:
s1, continuously breeding an intraspecific mixed family of Babylonia tussilago and Babylonia quadricarina, and taking the Babylonia tussilago as a seed;
s2, performing gonad ripening treatment and interspecific hybridization pairing on the seed snails obtained in the step S1 to obtain interspecific hybridization paired oocysts;
S3, carrying out scale breeding on oocysts obtained by the inter-seed hybridization pairing in the step S2 to obtain inter-seed first-filial generation offspring seeds, and culturing the inter-seed first-filial generation offspring seed populations until finished products are obtained to obtain the first-filial generation Babylonia;
S4, culturing the first-generation Babylonia of the hybrid in the step S3 until gonad is mature, inducing spawning, carrying out population mixing in the family to obtain oocysts, and incubating the oocysts to obtain a second-generation hybrid seed population; culturing the hybrid second-generation seedling population to a finished product, and taking the weight and the shell length as screening standards, wherein the screened family is the hybrid second-generation Babylonia;
S5, carrying out population mixing propagation on the second generation Babylonia of the hybrid in the family, and continuously breeding for multiple generations to obtain the hybrid Babylonia with strong disease resistance and long speed.
2. The interspecific hybridization method according to claim 1, wherein in the step S1, the continuous breeding is to select 2 or more generations of intraspecific hybrid families.
3. The interspecific crossing method according to claim 1, wherein in said step S1, said breeding is performed by taking body weight, shell length and survival rate as target traits, and cutting off and retaining the offspring of intraspecific crossing at a selection pressure of 10%.
4. The interspecific hybridization method according to claim 1, wherein in the step S2, the interspecific hybridization pair is a single pair hybridization.
5. The interspecific hybridization method according to claim 1, wherein in said step S2, said gonadal maturation process is: the green light or blue light is set in the external environment, and the light intensity is controlled to be 16-32 mu mol.m -2·s-1.
6. The interspecific hybridization method according to claim 1, wherein in the step S3, the conditions for the large-scale breeding are as follows: the illumination is 500-1000 lx, the salinity is 20-34, and the water temperature is 22-31 ℃.
7. The interspecific hybridization method according to claim 1, wherein in step S4, the fed bait is mixed with semen astragali Complanati extract during the period from the cultivation of the first filial generation Babylonia to gonad maturation.
8. The interspecific hybridization method according to claim 7, wherein said semen astragali Complanati extract is prepared by: reflux extracting semen astragali Complanati with ethanol solution under heating, vacuum filtering, concentrating under reduced pressure to obtain extract, and drying to obtain semen astragali Complanati extract.
9. The hybrid Babylonia with strong disease resistance and long and fast speed obtained by breeding the mud Babylonia and the Babylonia quadricarina by the interspecific hybridization method of any one of claims 1 to 8.
10. The use of the interspecific hybridization method of Babylonia in accordance with any one of claims 1 to 8 for breeding hybrid Babylonia with strong disease resistance and long speed.
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| CN104798709A (en) * | 2015-04-22 | 2015-07-29 | 海南定利养殖种苗有限公司 | Breeding method of babylonia areolata cross-breeding seeds |
| CN110178770A (en) * | 2019-06-20 | 2019-08-30 | 厦门大学 | A kind of Babylonia family is established and fine-variety breeding method |
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| CN116439174A (en) * | 2023-03-15 | 2023-07-18 | 广东海洋大学 | Seedling cultivation method for Babylonia |
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| CN104798709A (en) * | 2015-04-22 | 2015-07-29 | 海南定利养殖种苗有限公司 | Breeding method of babylonia areolata cross-breeding seeds |
| CN110178770A (en) * | 2019-06-20 | 2019-08-30 | 厦门大学 | A kind of Babylonia family is established and fine-variety breeding method |
| CN110839572A (en) * | 2019-12-13 | 2020-02-28 | 福建省洋泽海洋生物科技有限公司 | Artificial seedling culture method for babylonia areolata |
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