CN112535128A - Method for producing allotetraploid by hybridizing diploid of Crassostrea ampelopsis Grossdentata and triploid of Crassostrea pacifica - Google Patents
Method for producing allotetraploid by hybridizing diploid of Crassostrea ampelopsis Grossdentata and triploid of Crassostrea pacifica Download PDFInfo
- Publication number
- CN112535128A CN112535128A CN202010673186.2A CN202010673186A CN112535128A CN 112535128 A CN112535128 A CN 112535128A CN 202010673186 A CN202010673186 A CN 202010673186A CN 112535128 A CN112535128 A CN 112535128A
- Authority
- CN
- China
- Prior art keywords
- oyster
- breeding
- diploid
- triploid
- larvae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for producing oyster allotetraploid by hybridizing grapevine oyster diploid and pacific oyster triploid. The invention takes Pacific oyster triploid as a female parent, takes full weight as a target, preferably uses portuguese oyster diploid as a male parent, and produces oyster allotetraploid through drug-induced hybridization. The oyster allotetraploid obtained by the method has the characteristic of wide temperature, and the oyster triploid produced by hybridization with the common portuguese oyster or the pacific oyster diploid is suitable for coastal culture in south and north China, and has wide popularization and application prospects.
Description
Technical Field
The invention relates to a method for producing heterotetraploid by shellfish hybridization, in particular to a method for producing oyster heterotetraploid by hybridizing a portuguese oyster and a pacific oyster.
Background
Oyster, as an important marine organism, has delicious meat taste and high nutritional value and is called 'milk in the sea'. The oysters have wide geographical distribution, fast growth, high yield and high economic value, and are important mariculture objects in all countries in the world.
The growth advantage of the triploid oyster is obvious. Triploid oysters have 3 sets of chromosomes and are therefore extremely low in fertility, which means that on the one hand they can grow faster (their energy is used for growth and not for reproduction), and on the other hand they produce no or very little gametes during the summer, which ensures a stable quality. Triploid oysters are composed of tetraploid oysters and diploid oysters that are crossed, and thus it is important to obtain culturable tetraploid parents.
The traditional tetraploid oyster preparation all adopts homologous species oysters, wherein:
the crassostrea viticola serves as a main crassostrea gigas breeding variety in the south China coastal region, the growth speed is high, the breeding period is short, and the breeding risk is low. However, the oyster shells are thin and crisp, the meat yield is low, the selling price is low and the like because the growth speed of the portuguese oysters is too high.
The Pacific oyster is used as a main oyster breeding variety in coastal areas in northern China, has firm oyster shell and high meat yield, can be eaten raw so as to enter a high-end market, and has considerable value. However, the pacific oyster cultivation period is too long, the pacific oyster can be generally listed after 2-3 years, the survival rate is low, and the cultivation risk is increased. The pacific oysters, as low-temperature species, cannot adapt to the high-temperature climate in the south in summer, and therefore cannot be cultivated in the south coast of China all the year round.
The allopolyploid is a polyploid formed by chromosome doubling of hybrid offspring generated by hybridization of different species, and has a whole set of chromosome sets of two or more different species and stronger natural selection advantage. Therefore, the heterotetraploid is bred by hybridization of the Ostrea vittata and the Ostrea pacifica, so that heterosis is utilized, and the problems of the two oysters can be effectively solved.
Disclosure of Invention
Technical problem to be solved
Traditional tetraploid oyster preparation uses homologous species oysters: the growth speed of the ostrea viticola is too high, so that the oyster shells are thin and crisp, the meat yield is not high, the culture period of the pacific oyster is too long, the ostrea pacifica can be generally marketed in 2-3 years, the survival rate is low, and the culture risk is increased. Therefore, the invention performs hybridization on the two varieties to obtain the allotetraploid, obtains the heterosis and solves the inherent problems of oyster varieties.
(II) technical scheme
The method for producing the allotetraploid by hybridizing the diploid of the Crassostrea gigas and the triploid of the pacific oyster is characterized by comprising the following steps of:
(a) selecting Pacific oyster triploid as a breeding female parent, selecting common Portugal oyster diploid as a breeding male parent, and respectively putting the breeding scallops into scallop cultivation ponds for ripening;
(b) accelerating the maturation of the pacific oyster shell seeds until the gonads are mature, taking the eggs, and maturing the eggs before the next step; dissecting the mature-promoted grapevine oyster diploid to obtain sperms, and activating the sperms before the next step;
(c) mixing the mature ovum and the activated sperm in water, wherein the ratio of the number of the sperm to the number of the ovum is 5-10: 1, controlling the density of fertilized eggs to be less than or equal to 1 ten thousand/ml;
(d) observing the development condition of the fertilized eggs, and when 50-75% of the first polar body appears, rapidly adding cytochalasin B (0.5-1.0 mg/L) or 6-dimethylaminopurine (15-30 mg/L) for treatment for 20-30 min;
(e) cleaning and filtering the drug immediately after the drug treatment of the fertilized eggs, setting the hatching density, and placing the fertilized eggs in a black barrel for hatching; after the larvae grow to D-type larvae, screening the larvae, setting the breeding density and putting the larvae into a black barrel for breeding; and after the larvae have eyespots, putting the attaching matrix.
Preferably, in the step (a), the breeding female parent is large in individual, good in vitality and free of damage, the breeding male parent measures the total weight of all individuals, and 10% of the individuals in the top of the total weight are intercepted.
Preferably, in the step (a), the biological bait in the shellfish culture pond is artificially cultured golden algae, Chaetoceros and Platymonas verrucosa or Tuchi algae water.
Preferably, in the step (b), the curing time is 0.5-1 h, and the activation time is 10-20 min.
Preferably, in the step (c), the water temperature is kept at 25 ℃ and the salinity is kept at 25-30.
Preferably, in the step (e), the hatching density is kept between 1 and 3 per ml, and the breeding density is kept between 0.5 and 1 per ml.
(III) advantageous effects
The present invention utilizes heterosis between Portugal oyster and Pacific oyster and utilizes medicine to induce the hybridization between Portugal oyster diploid and Pacific oyster triploid to produce oyster allotetraploid, and has the features of wide temperature range. The oyster triploid produced by hybridizing the oyster allotetraploid with the common portuguese oyster or the pacific oyster diploid is suitable for breeding in coastal areas in south and north of China, and has wide popularization and application prospects.
Drawings
FIG. 1 is a diagram of a common oyster diploid peak;
FIG. 2 is a graph of the peak values of larvae of D-type heterotetraploid of oysters produced by the method of the invention;
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-2, the present invention provides an embodiment;
example 1:
(a) selection and ripening of breeding shellfish: 200 triploids (with the shell height of 10-15 cm) of triploid of tripartite Pacific oyster with large individual, good vitality and no damage are selected as breeding female parents, and 10% of individuals with the top of the total weight are cut from 2000 diploids (with the shell height of 8-12 cm) of Portuguese oyster with the second age as breeding male parents. And then putting the breeding shells into a breeding shell culture pond respectively to promote the maturity by adopting a running water method, wherein the biological bait mainly comprises artificially cultured golden algae, Chaetoceros and Platymonas subcordiformis, and the soil pond algae water is used as a standby bait source.
(b) Obtaining triploid ovum: after the gonads of the pacific oyster species are matured, 50 pacific oyster triploid individuals are detected by a ploidy analyzer, 48 3N individuals are detected in total, and 20 female 3N individuals with full glands and good ovum development are screened out as female parents through microscope observation. Taking out soft parts of 20 female parents one by one, cleaning, extruding out ova, filtering large-particle impurities of the ova by using a 100-mesh silk net, filtering mucus by using a 500-mesh silk net, and placing the ova liquid in a 1L plastic cup. Placing the filtered egg liquid in seawater with salinity of 25 for aging for 0.5h, and performing next fertilization after the ovum becomes round;
obtaining diploid sperms: dissecting 40 diploid of the grapevine oyster, distinguishing male and female by using a microscope, selecting 15 individuals with plump gonads and strong sperm motility as male parents, extruding the sperms 10min before the start of artificial fertilization, filtering impurities by using a 500-mesh silk net, placing in seawater with salinity of 25 for activation, and detecting that the sperm motility is more than 90% under the microscope.
(c) Artificial insemination: according to a one-to-one principle, 20 parts of cured Pacific oyster triploid egg liquid is respectively added with activated grapevine oyster diploid sperms, the number ratio of sperms to eggs is 5:1, the density of fertilized eggs is kept to be less than or equal to 1 ten thousand/ml, the egg liquid is continuously stirred, the water temperature is kept to be about 25 ℃, and the salinity is 25.
(d) Drug induction: note that the development of fertilized eggs was observed, and when 50% of the first polar body appeared, 1.0mg/L cytochalasin B was added rapidly and treated for 30 min.
(e) Larva breeding: immediately washing fertilized eggs in 1% dimethyl sulfoxide for 2 times after the drug treatment is finished, soaking for 15min each time, filtering with a 500-mesh silk net to remove the drug, then placing fertilized egg liquid in 20 black barrels of 500L respectively for hatching, keeping the hatching density at 1/ml, and slightly inflating. When fertilized eggs develop into D-type larvae, a ploidy analyzer is used for detecting the proportion of 4N, 15 groups with high induced proportion of 4N are left, and 400-mesh silk nets are used for collecting the D-type larvae and putting the D-type larvae into 15 disinfected black barrels for cultivation. The larva breeding density is kept at 0.5/ml, and artificially cultured golden algae and chaetoceros are fed. 6 groups of 15 groups of larvae die in the larva breeding process, the remaining 9 groups of larvae develop to eyespots, the 9 groups of larvae are collected together and put into a black barrel, and oyster shells are put into the black barrel as an attachment substrate.
Example 2:
(a) selection and ripening of breeding shellfish: 200 triploids (with the shell height of 10-15 cm) of triploid of tripartite Pacific oyster with large individual, good vitality and no damage are selected as breeding female parents, and 10% of individuals with the top of the total weight are cut from 2000 diploids (with the shell height of 8-12 cm) of Portuguese oyster with the second age as breeding male parents. And then putting the breeding shells into a breeding shell culture pond respectively to promote the maturity by adopting a running water method, wherein the biological bait mainly comprises artificially cultured golden algae, Chaetoceros and Platymonas subcordiformis, and the soil pond algae water is used as a standby bait source.
(b) Obtaining triploid ovum: after the gonads of oyster species are matured, 50 pacific oyster triploid individuals are detected by a ploidy analyzer, 45 3N individuals are detected in total, and 16 female 3N individuals with plump glands and good ovum development are screened out as female parents through microscope observation. Taking out soft parts of 16 female parents one by one, cleaning, extruding out ova, filtering large-particle impurities of the ova by using a 100-mesh silk net, filtering mucus by using a 500-mesh silk net, and placing the ova liquid in a 1L plastic cup. Placing the filtered egg liquid in seawater with salinity of 30 for curing for 1h, and performing next fertilization after the ovum becomes round;
obtaining diploid sperms: dissecting 40 diploid of the grapevine oyster, distinguishing male and female by using a microscope, selecting 8 individuals with plump gonads and strong sperm motility as male parents, extruding the sperms 20min before the start of artificial fertilization, filtering impurities by using a 500-mesh silk net, placing in seawater with salinity of 30 for activation, and detecting that the sperm motility is more than 90% under the microscope.
(c) Artificial insemination: according to a one-to-one principle, 16 parts of cured Pacific oyster triploid egg liquid is respectively added with activated grapevine oyster diploid sperms, the number ratio of sperms to eggs is 10:1, the density of fertilized eggs is kept to be less than or equal to 1 ten thousand/ml, the egg liquid is continuously stirred, the water temperature is kept to be about 25 ℃, and the salinity is 30.
(d) When 75% of the first polar body appears, 30mg/L of 6-dimethylaminopurine is rapidly added for 20 min.
(e) Larva breeding: immediately washing fertilized eggs in 1% dimethyl sulfoxide for 2 times after the drug treatment is finished, soaking for 15min each time, filtering with a 500-mesh silk net to remove the drug, then placing fertilized egg liquid in 16 black barrels of 500L respectively for hatching, keeping the hatching density at 3/ml, and slightly inflating. When fertilized eggs develop into D-type larvae, a ploidy analyzer is used for detecting the proportion of 4N, 12 groups with higher induced proportion of 4N are left, and a 400-mesh silk net is used for collecting the D-type larvae and putting the D-type larvae into 12 disinfected black barrels for cultivation. The larva breeding density is kept at 1/ml, and artificially cultured golden algae and chaetoceros are fed. 4 groups of 12 groups of larvae die in the larva breeding process, the remaining 8 groups of larvae develop to eyespots, the 8 groups of larvae are collected together and put into a black barrel, and oyster shells are put into the black barrel as an attaching matrix.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The method for producing the allotetraploid by hybridizing the diploid of the Crassostrea gigas and the triploid of the pacific oyster is characterized by comprising the following steps of:
(a) selecting Pacific oyster triploid as a breeding female parent, selecting common Portugal oyster diploid as a breeding male parent, and respectively putting the breeding scallops into scallop cultivation ponds for ripening;
(b) accelerating the maturation of the pacific oyster shell seeds until the gonads are mature, taking the eggs, and maturing the eggs before the next step; dissecting the mature-promoted grapevine oyster diploid to obtain sperms, and activating the sperms before the next step;
(c) mixing the mature ovum and the activated sperm in water, wherein the ratio of the number of the sperm to the number of the ovum is 5-10: 1, controlling the density of fertilized eggs to be less than or equal to 1 ten thousand/ml;
(d) observing the development condition of the fertilized eggs, and when 50-75% of the first polar body appears, rapidly adding cytochalasin B (0.5-1.0 mg/L) or 6-dimethylaminopurine (15-30 mg/L) for treatment for 20-30 min;
(e) cleaning and filtering the drug immediately after the drug treatment of the fertilized eggs, setting the hatching density, and placing the fertilized eggs in a black barrel for hatching; after the larvae grow to D-type larvae, screening the larvae, setting the breeding density and putting the larvae into a black barrel for breeding; and after the larvae have eyespots, putting the attaching matrix.
2. The method of claim 1, wherein: in the step (a), the breeding female parent is large in individual, good in vitality and free of damage, the breeding male parent measures the total weight of all individuals, and 10% of the individuals in the top of the total weight are intercepted.
3. The method of claim 1, wherein: in the step (a), the biological bait in the shellfish culture pond is artificial culture water of chrysophyceae, chaetoceros and platymonas or euglena.
4. The method of claim 1, wherein: in the step (b), the curing time is 0.5-1 h, and the activation time is 10-20 min.
5. The method of claim 1, wherein: in the step (c), the water temperature is kept at 25 ℃, and the salinity is kept at 25-30.
6. The method of claim 1, wherein: in the step (e), the hatching density is kept at 1-3/ml, and the breeding density is kept at 0.5-1/ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010673186.2A CN112535128A (en) | 2020-07-14 | 2020-07-14 | Method for producing allotetraploid by hybridizing diploid of Crassostrea ampelopsis Grossdentata and triploid of Crassostrea pacifica |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010673186.2A CN112535128A (en) | 2020-07-14 | 2020-07-14 | Method for producing allotetraploid by hybridizing diploid of Crassostrea ampelopsis Grossdentata and triploid of Crassostrea pacifica |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112535128A true CN112535128A (en) | 2021-03-23 |
Family
ID=75013456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010673186.2A Withdrawn CN112535128A (en) | 2020-07-14 | 2020-07-14 | Method for producing allotetraploid by hybridizing diploid of Crassostrea ampelopsis Grossdentata and triploid of Crassostrea pacifica |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112535128A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114208734A (en) * | 2021-12-22 | 2022-03-22 | 中国科学院南海海洋研究所 | Method for improving production performance of portuguese oyster triploid by integrating multiple breeding technologies |
| CN114600807A (en) * | 2022-04-02 | 2022-06-10 | 厦门大学 | Method for inducing Haliotis discus hannai tetraploid |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1144454A (en) * | 1994-01-21 | 1997-03-05 | 洛特格斯新泽西州立大学 | Tetraploid shellfish |
| CN1545873A (en) * | 2003-12-16 | 2004-11-17 | 中国海洋大学 | Method for inducting oyster tetraploid using 6-dimethylin purine |
| US7013836B1 (en) * | 2002-06-27 | 2006-03-21 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Enhancing maturation of oocytes in bivalves |
| CN102573453A (en) * | 2009-08-14 | 2012-07-11 | 西亚益维株式会社 | Method for producing viable tetraploid oysters |
| CN102939916A (en) * | 2012-10-19 | 2013-02-27 | 汕头大学 | EPA content-increasing oyster breeding method and application thereof |
| CN107494358A (en) * | 2017-09-28 | 2017-12-22 | 中国科学院南海海洋研究所 | A kind of preparation method of Hong Kong oyster tetraploid children shellfish |
| CN109730008A (en) * | 2019-03-11 | 2019-05-10 | 中国海洋大学 | A method of cultivating Portuguese oyster tetraploid |
| US20190281800A1 (en) * | 2018-03-14 | 2019-09-19 | Rutgers, The State University Of New Jersey | Molluscan shellfish produced by controlled crossbreeding |
-
2020
- 2020-07-14 CN CN202010673186.2A patent/CN112535128A/en not_active Withdrawn
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1144454A (en) * | 1994-01-21 | 1997-03-05 | 洛特格斯新泽西州立大学 | Tetraploid shellfish |
| US7013836B1 (en) * | 2002-06-27 | 2006-03-21 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Enhancing maturation of oocytes in bivalves |
| CN1545873A (en) * | 2003-12-16 | 2004-11-17 | 中国海洋大学 | Method for inducting oyster tetraploid using 6-dimethylin purine |
| CN102573453A (en) * | 2009-08-14 | 2012-07-11 | 西亚益维株式会社 | Method for producing viable tetraploid oysters |
| CN102939916A (en) * | 2012-10-19 | 2013-02-27 | 汕头大学 | EPA content-increasing oyster breeding method and application thereof |
| CN107494358A (en) * | 2017-09-28 | 2017-12-22 | 中国科学院南海海洋研究所 | A kind of preparation method of Hong Kong oyster tetraploid children shellfish |
| US20190281800A1 (en) * | 2018-03-14 | 2019-09-19 | Rutgers, The State University Of New Jersey | Molluscan shellfish produced by controlled crossbreeding |
| CN109730008A (en) * | 2019-03-11 | 2019-05-10 | 中国海洋大学 | A method of cultivating Portuguese oyster tetraploid |
Non-Patent Citations (2)
| Title |
|---|
| 施坤涛等: "四倍体太平洋牡蛎(Crassostrea gigas Thunberg)自群繁殖的研究", 《海洋湖沼通报》 * |
| 迟长凤等: "扇贝异源四倍体诱导的初步研究", 《水产学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114208734A (en) * | 2021-12-22 | 2022-03-22 | 中国科学院南海海洋研究所 | Method for improving production performance of portuguese oyster triploid by integrating multiple breeding technologies |
| CN114600807A (en) * | 2022-04-02 | 2022-06-10 | 厦门大学 | Method for inducing Haliotis discus hannai tetraploid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102301969B (en) | Method for cultivating hybridized and backcrossed marine product fries | |
| CN101703016B (en) | Technology for culturing unisexual hybrid scallop by utilizing purple scallop and bay scallop | |
| CN103858803B (en) | The selection of the long oyster new lines of a kind of shell gold | |
| CN102100197A (en) | Method for breading triploid monomer oysters | |
| JP5543583B2 (en) | How to cultivate orange scallop scallop | |
| CN101990854A (en) | Breeding method of pure color breeding line of pacific oyster shells | |
| CN101317550A (en) | Artificial nursery stock growing method for hybridized mussel | |
| CN113016673A (en) | Method for producing oyster of Fujian oyster of yellow shell/black shell triploid in large scale | |
| CN101926293B (en) | Breeding method of clam fast growth strain | |
| CN112535128A (en) | Method for producing allotetraploid by hybridizing diploid of Crassostrea ampelopsis Grossdentata and triploid of Crassostrea pacifica | |
| CN114208734B (en) | Method for improving production performance of portuguese oyster triploid by integrating multiple breeding technologies | |
| CN101120665A (en) | Seedling culture method for fresh water pearl culturing clam | |
| AU2021104593A4 (en) | Cultivation method of first-generation commercial seed hybrids of female Patinopecten caurinus and male Patinopecten yessoensis | |
| CN114208735B (en) | Method for cultivating rapid-growth new strain of hong Kong oyster triploid by backcross breeding technology | |
| CN106797899B (en) | Method for cultivating new strain of ostrea gigas with high temperature resistance in north | |
| CN112931323A (en) | Induction method of high heterozygosity tetraploid of novel variety Haoda No. 3 of crassostrea gigas | |
| CN112535127A (en) | Method for producing allotetraploid by hybridizing diploid of Crassostrea ampelopsis Grossdentata and triploid of Crassostrea hongkongensis | |
| CN108522378B (en) | Cultivation method of first-filial-generation commercial offspring seeds of female Arragana scallop and male Patinopecten yessoensis | |
| JP2008125422A (en) | Method for converting dried laver seaweed into single cell form, and culturing the same | |
| CN113951191A (en) | Method for breeding good oyster seedlings by utilizing oyster triploid | |
| CN108541636B (en) | Method for breeding new hybrid variety of female Arragana scallop and male Patinopecten yessoensis | |
| CN108244007B (en) | Method for breeding new hybrid variety of female patinopecten yessoensis and male alaska scallop | |
| CN105815246A (en) | Hybrid seed production method for Argopecten irradians concentricus (female) and Chlamys nobilis (male) | |
| CN112136736A (en) | Breeding method of stress-resistant crassostrea gigas new variety | |
| CN105766726A (en) | Chlamys nobilis (female) and Argopecten irradians concentricus Say (male) hybrid breeding method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20210323 |
|
| WW01 | Invention patent application withdrawn after publication |