CN101990854A - Breeding method of pure color breeding line of pacific oyster shells - Google Patents
Breeding method of pure color breeding line of pacific oyster shells Download PDFInfo
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- CN101990854A CN101990854A CN2010105372553A CN201010537255A CN101990854A CN 101990854 A CN101990854 A CN 101990854A CN 2010105372553 A CN2010105372553 A CN 2010105372553A CN 201010537255 A CN201010537255 A CN 201010537255A CN 101990854 A CN101990854 A CN 101990854A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
A breeding method of pure color breeding line of pacific oyster shells is characterized in that two to three years old individuals with regular shapes in which the shell height is 10-15cm are selected as parents by using absolute black left shell, absolute white left shell and golden right shell as marks in the pacific oyster individuals in natural population; the parents with matured gonad are dissected to distinguish male and female; male and female gametes are respectively selected to filter and remove impurities through 200-260 meshes dip net and disposed in different containers; the obtained male and female gametes respectively perform mass mating and single pair mating; an isolating device is used for performing larval training; intermediate breeding is performed before the larval setting stage of the natural pacific oyster in natural sea area is finished; each breeding line is respectively marked and disposed in the same sea area for breeding. In the invention, the conventional breeding production method of pacific oyster is used when in application without extra added cost, and the method can be used in large-scale production.
Description
Technical field
The present invention relates to a kind of shellfish and select the method for breeding, be specifically related to a kind of producing method for seed of long oyster shell colour purity look breeding line.
Background technology
Color because it has the concern that hereditability is subjected to geneticist, breeding scholar always, occupies consequence as an important economical trait of breeding biology in the animals and plants genetic breeding.In the aquatic livestock, most of color proterties belongs to the simple relatively inherited characteristics of hereditary pattern, as red men and the body colour of " plain boiled pork ", bright and beautiful carp (Cryprinus carpiod) and Tilapia mossambica (Tilapia sp.), the silverside (Oncorhynchus kisutch) of genus quantitative character and the body colour of Atlantic salmon (Salmosalar) of the king salmon muscle that belongs to qualitative character.In selecting breeding work, when a certain proterties has the minority gene and simply show the decision of recessiveness mechanism, the purpose proterties is separated by suitable mating design, just can obtain pure lines (Falconer, 1996) through the constant generations selection.The selection breeding work of carrying out according to this character pair aquatic livestock of color has been obtained certain progress both at home and abroad.Aspect fish breeding, Wu Qingjiang etc. (1980) go out the red lens carp by hybridization and the continuous selfed breeding of carp; Aspect the shellfish breeding, Wada and Komaru (1990) have selected high white shell pearl shell (the Pinctada fucata martensii) strain of pearl quality, Zhang Guofan etc. (2005) have selected orange shell look " middle section is red " bay scallop, and He Maoxian etc. (2006) select the red shell chromaticity of pteria martensii (Pinctada martensii) system etc.
Long oyster (Crassostrea gigas) natural distribution is to culture seashells widest in area, that output is maximum in the world in Japan, China and Korea S, and China's oyster output accounts for 81% of world's oyster output, wherein is mainly long oyster.The genetic improvement of long oyster is operated in the U.S., France, Australia and China extensively to be carried out, and has obtained remarkable effect at disease-resistant, high yield, aspect such as degeneration-resistant.When long oyster was sold with live body, the consumer often had different preferences to the color of shell of oyster.Studies show that the left shell look of long oyster to the black continuous distribution, belongs to the quantitative character by complex gene control by pure white, has higher heritability (Evans etc., 2009; Li Qi etc., 2009).In addition, the applicant has found that in length oyster natural population the right shell of ratio very low (about 0.3%) is golden long oyster, and this proterties also has higher hereditary capacity.Therefore, carry out the seed selection of different shell looks long oyster strain, not only can satisfy different consumer demands, also can improve the value of long oyster product.
Summary of the invention
The purpose of this invention is to provide a kind of producing method for seed of long oyster shell colour purity look breeding line, it can satisfy the demand of prior art.
A kind of producing method for seed of long oyster shell colour purity look breeding line is characterized in that earlier being mark with left shell black, the pure white and right shell gold of left shell from the long oyster individuality of natural population, select 2～3 ages, the high 10～15cm of shell, hull shape regular individualities as the parent; The parent of gonadal maturation is dissected, distinguish female, male, get female, andro gamete respectively, filter with 200～260 orders diddle-net and remove impurity, place different vessels; Female, the andro gamete that obtain are carried out mass mating and single pair mating respectively; Take spacer assembly to carry out young cultivation; Before the natural long oyster young setting stage of natural waters finishes, carry out intermediate rearing; Each breeding line of mark places same sea area to form respectively.
The present invention is a mark with long oyster shell look proterties, adopts traditional selection, and the color that makes left shell and right shell can be cultivated the long oyster shell colour purity look breeding line of pure white, left shell black of left shell and right shell gold respectively at short-term generation interior orientation purifying.The present invention adopts the conventional seedling and seed production process of long oyster to get final product when using, and need not additionally to increase cost, aborning large-scale application.
Embodiment
The producing method for seed of long oyster shell colour purity look breeding line of the present invention, it is characterized in that earlier from the long oyster individuality of natural population, with left shell black, the pure white and right shell gold of left shell is mark, selects 2～3 ages, the high 10～15cm of shell, hull shape regular individualities as the parent; The parent of gonadal maturation is dissected, distinguish female, male, get female, andro gamete respectively, filter with 200～260 orders diddle-net and remove impurity, place different vessels; Female, the andro gamete that obtain are carried out mass mating and single pair mating respectively; Take spacer assembly to carry out young cultivation; Before the natural long oyster young setting stage of natural waters finishes, carry out intermediate rearing; Each breeding line of mark places same sea area to form respectively.
The invention will be further described below in conjunction with embodiment:
The seed selection of the 1st generation
1. the parent selects: in May, 2008, select 2 ages, the average high 13cm of shell, the regular individuality of hull shape from the cultured population of length oyster Weihai, choosing left shell look is that black and pure white, right shell look are each 100 conduct breedings of golden individuality parent.
2. gamete obtains: the parent of gonadal maturation is dissected, distinguishes male and female, filter with 200 orders diddle-net and remove impurity, female, the andro gamete that obtains is collected in respectively in the good container of mark.
Described container is the vinyon bucket of 5～10L.
3. fertilization and hatching: female, the andro gamete of choosing pure white, the left shell black of left shell, the golden parent of right shell carry out mass mating, guarantee that the usage amount of each parent's oogamete and andro gamete is identical; Female, the male parent of choosing pure white, the left shell black of left shell, right shell gold carry out single pair mating.Each group fertilized egg is put into respectively in the 100L vinyon bucket that fills fresh seawater, the trace inflation, hatching density is controlled at 40/ml, under 24 ℃ of conditions of water temperature, all hatches to D shape larva through about 24h.
Described mass mating is to do to mix mating with each 6～15 of parents; Described single pair mating is that each 1 of female, male parent is made single pair mating.
4. larval culture: with 300 mesh sieve thin,tough silk diddle-net with above-mentioned D shape larva select excellent after, place the device of mutual isolation to cultivate respectively each family.Cultivate density according to the vegetative stage adjustment: embryophore length is during less than 120 μ m, 15/ml; When embryophore reaches 160 μ m, 3/ml; When embryophore reached 260 μ m, density was reduced to 1/ml.In the larval culture process, equilateral chrysophyceae of throwing something and feeding (Isochrysis galbana) and flat algae (Platymonas sp.), water temperature is 24 ℃, salinity is 30.
Described spacer assembly is 100～200L vinyon bucket fully independently and each self-corresponding water diddle-net, bait throwing in beaker of changing.
5. intermediate rearing: the breeding line of settlement and metamorphosis is carried out intermediate rearing, respectively behind the marks, place the water body of the no natural long oyster young to cultivate different breeding lines.
Described intermediate rearing is that each breeding line of mark places outdoor water body or indoor water body respectively, cultivates to the juvenile mollusk of the long 5～10mm of shell.
6. form at the sea: the setting stage (6～August) for the treatment of the natural long oyster young of natural waters finishes, and will place breeding cage respectively behind each breeding line mark, and form in same sea area, periodic cleaning aufwuch between mature stage, and measure growing state.
The envirment factor that described same sea area is the sea area is identical with the breeding way of oyster.
Step such as form through parent's selection, parent's accelerating, artificial insemination and hatching, young cultivation, intermediate rearing, sea, obtained the 1st a generation left side pure white, the left shell black of shell and the breeding line of right shell gold.In the 1st generation of mid-June in 2009, grow to sexual maturity, can carry out the seed selection of the 2nd generation.
The seed selection of the 2nd generation
1. the parent selects: in June, 2009, a long oyster left side pure white, left shell black of setting up from 2008 of shell and the golden breeding line of right shell are selected the regular individuality of shell mould respectively the 1st generation, and each 100 as the 2nd generation parent.
2. each step is formed all with the seed selection of the 1st generation in acquisition female and male gametophyte, artificial insemination and hatching, young cultivation, intermediate rearing, sea.
3.2009 year early June, the 1st generation breeding line sexual maturity that embodiment 1 obtains, by embodiment 1 each link, obtained for the 2nd generation, left side shell pure white, left shell black, right shell gold are further purified respectively, to the 2nd generation of May in 2010 the breeding line individual growth to shell look fixedly the time, left shell white purifying rate reaches 82.4%, left side shell black purifying rate reaches 69.2%, and the golden purifying rate of right shell has reached 90.5%.2008～2010 years, the long oyster shell colour purity chromaticity that successfully selects the 1st, 2 generations left sides pure white, left shell black of shell and right shell gold was.Compare with control group, the pure white and left shell black of left shell strain has grow fast, dressing percentage advantages of higher, and the golden strain of right shell has the fast characteristics of shell colour purityization.
The present invention utilizes long oyster shell look proterties to have characteristic than high heritability, method by colony's seed selection and family selective breeding, make long oyster left side shell black, left shell white and right shell gold at short-term interior orientation purifying from generation to generation, set up the pure color breeding line producing method for seed of the black and right shell coloured gold look of white, the left shell colour purity of long oyster left side shell colour purity.
Claims (6)
1. the producing method for seed of one kind long oyster shell colour purity look breeding line is characterized in that earlier being mark with left shell black, the pure white and right shell gold of left shell from the long oyster individuality of natural population, select 2～3 ages, the high 10～15cm of shell, hull shape regular individualities as the parent; The parent of gonadal maturation is dissected, distinguish female, male, get female, andro gamete respectively, filter with 200～260 orders diddle-net and remove impurity, place different vessels; Female, the andro gamete that obtain are carried out mass mating and single pair mating respectively; Take spacer assembly to carry out young cultivation; Before the natural long oyster young setting stage of natural waters finishes, carry out intermediate rearing; Each breeding line of mark places same sea area to form respectively.
2. producing method for seed as claimed in claim 1 is characterized in that described container is the vinyon bucket of 5～10L.
3. producing method for seed as claimed in claim 1 is characterized in that described mass mating is for doing to mix mating with each 6～15 of parents; Described single pair mating is that each 1 of female, male parent is made single pair mating.
4. producing method for seed as claimed in claim 1 is characterized in that described spacer assembly is 100～200L vinyon bucket fully independently and each self-corresponding water diddle-net, bait throwing in beaker of changing.
5. producing method for seed as claimed in claim 1 is characterized in that described intermediate rearing is that each breeding line of mark places outdoor water body or indoor water body respectively, cultivates to the juvenile mollusk of the long 5～10mm of shell.
6. producing method for seed as claimed in claim 1 is characterized in that the breeding way of envirment factor that described same sea area is the sea area and oyster is identical.
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| CN2010105372553A CN101990854A (en) | 2010-10-29 | 2010-10-29 | Breeding method of pure color breeding line of pacific oyster shells |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102499151A (en) * | 2011-11-04 | 2012-06-20 | 大连獐子岛渔业集团股份有限公司 | Breeding method of new high-yield stress-resistant red shell color scallop line |
| CN102742532A (en) * | 2012-07-31 | 2012-10-24 | 中国海洋大学 | Cultivation method of novel strain of crassostrea gigas with pure purple left shells |
| CN102939916A (en) * | 2012-10-19 | 2013-02-27 | 汕头大学 | EPA content-increasing oyster breeding method and application thereof |
| CN103004648A (en) * | 2011-09-22 | 2013-04-03 | 汕头大学 | Breeding method for improving DHA (Docosahexaenioc Acid) content in oyster body |
| WO2013123708A1 (en) * | 2012-02-23 | 2013-08-29 | 中国海洋大学 | Method for sampling crassostrea gigas non-destructively |
| CN103416336A (en) * | 2013-08-16 | 2013-12-04 | 中国海洋大学 | Breeding method for new strain of crassostrea gigas with orange left and right shells |
| CN103651223A (en) * | 2014-01-03 | 2014-03-26 | 鲁东大学 | Method for evaluating green weight of soft part of ostrea gigas thunb |
| CN103749357A (en) * | 2014-01-08 | 2014-04-30 | 中国科学院南海海洋研究所 | Method of producing seeds of Crassostrea hongkongensis by using gill aperture freeness as marker |
| CN103975879A (en) * | 2014-05-12 | 2014-08-13 | 福建省水产研究所 | Cultivation method of new strain of quickly-grown Portuguese oysters with golden yellow shells |
| CN105210946A (en) * | 2015-10-13 | 2016-01-06 | 中国科学院南海海洋研究所 | A kind of with the Kumamoto oyster producing method for seed of right shell color for mark |
| CN105360022A (en) * | 2015-10-13 | 2016-03-02 | 中国科学院南海海洋研究所 | Seed production method of double gold shell Crassostreasikameastrain |
| CN105360023A (en) * | 2015-10-13 | 2016-03-02 | 中国科学院南海海洋研究所 | Seed production method of gold adductor muscle scar Kumamoto oyster line |
| CN106577415A (en) * | 2016-12-26 | 2017-04-26 | 中国海洋大学 | Seed production method of black-shell pacific oyster new strain |
| CN106614164A (en) * | 2016-12-26 | 2017-05-10 | 中国海洋大学 | Cultivation method for new strain of pure white shell pacific oyster |
| CN106804492A (en) * | 2017-01-19 | 2017-06-09 | 中国海洋大学 | The measuring method of one seed oyster parent's shellfish gonad maturity and application |
| CN113331096A (en) * | 2021-06-07 | 2021-09-03 | 湛江华南贝类研究院 | Settleable crassostrea gigas population breeding method |
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Cited By (26)
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| CN103004648A (en) * | 2011-09-22 | 2013-04-03 | 汕头大学 | Breeding method for improving DHA (Docosahexaenioc Acid) content in oyster body |
| CN103004648B (en) * | 2011-09-22 | 2014-09-17 | 汕头大学 | Breeding method for improving DHA (Docosahexaenioc Acid) content in oyster body |
| CN102499151A (en) * | 2011-11-04 | 2012-06-20 | 大连獐子岛渔业集团股份有限公司 | Breeding method of new high-yield stress-resistant red shell color scallop line |
| CN102499151B (en) * | 2011-11-04 | 2015-06-17 | 獐子岛集团股份有限公司 | Breeding method of new high-yield stress-resistant red shell color scallop line |
| WO2013123708A1 (en) * | 2012-02-23 | 2013-08-29 | 中国海洋大学 | Method for sampling crassostrea gigas non-destructively |
| CN102742532A (en) * | 2012-07-31 | 2012-10-24 | 中国海洋大学 | Cultivation method of novel strain of crassostrea gigas with pure purple left shells |
| CN102939916A (en) * | 2012-10-19 | 2013-02-27 | 汕头大学 | EPA content-increasing oyster breeding method and application thereof |
| CN103416336B (en) * | 2013-08-16 | 2014-09-17 | 中国海洋大学 | Breeding method for new strain of crassostrea gigas with orange left and right shells |
| CN103416336A (en) * | 2013-08-16 | 2013-12-04 | 中国海洋大学 | Breeding method for new strain of crassostrea gigas with orange left and right shells |
| CN103651223B (en) * | 2014-01-03 | 2015-08-05 | 鲁东大学 | A kind of appraisal procedure of long oyster soft body weight in wet base |
| CN103651223A (en) * | 2014-01-03 | 2014-03-26 | 鲁东大学 | Method for evaluating green weight of soft part of ostrea gigas thunb |
| CN103749357A (en) * | 2014-01-08 | 2014-04-30 | 中国科学院南海海洋研究所 | Method of producing seeds of Crassostrea hongkongensis by using gill aperture freeness as marker |
| CN103975879A (en) * | 2014-05-12 | 2014-08-13 | 福建省水产研究所 | Cultivation method of new strain of quickly-grown Portuguese oysters with golden yellow shells |
| CN103975879B (en) * | 2014-05-12 | 2015-08-26 | 福建省水产研究所 | The breeding method of the golden yellow fast lady's fingers tooth oyster new lines of a kind of shell |
| CN105210946A (en) * | 2015-10-13 | 2016-01-06 | 中国科学院南海海洋研究所 | A kind of with the Kumamoto oyster producing method for seed of right shell color for mark |
| CN105360022A (en) * | 2015-10-13 | 2016-03-02 | 中国科学院南海海洋研究所 | Seed production method of double gold shell Crassostreasikameastrain |
| CN105360023A (en) * | 2015-10-13 | 2016-03-02 | 中国科学院南海海洋研究所 | Seed production method of gold adductor muscle scar Kumamoto oyster line |
| CN105360022B (en) * | 2015-10-13 | 2017-11-21 | 中国科学院南海海洋研究所 | A kind of producing method for seed of double golden shell Kumamoto oyster strains |
| CN106614164A (en) * | 2016-12-26 | 2017-05-10 | 中国海洋大学 | Cultivation method for new strain of pure white shell pacific oyster |
| CN106577415A (en) * | 2016-12-26 | 2017-04-26 | 中国海洋大学 | Seed production method of black-shell pacific oyster new strain |
| CN106577415B (en) * | 2016-12-26 | 2019-04-02 | 中国海洋大学 | A kind of producing method for seed of the long oyster new lines of black shell |
| CN106614164B (en) * | 2016-12-26 | 2019-04-30 | 中国海洋大学 | A kind of breeding method of the long oyster new lines of pure white shell |
| CN106804492A (en) * | 2017-01-19 | 2017-06-09 | 中国海洋大学 | The measuring method of one seed oyster parent's shellfish gonad maturity and application |
| CN106804492B (en) * | 2017-01-19 | 2019-08-13 | 中国海洋大学 | The measurement method and application of one seed oyster parent's shellfish gonad maturity |
| CN113331096A (en) * | 2021-06-07 | 2021-09-03 | 湛江华南贝类研究院 | Settleable crassostrea gigas population breeding method |
| CN113331096B (en) * | 2021-06-07 | 2022-06-03 | 湛江华南贝类研究院 | Settleable crassostrea gigas population breeding method |
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Application publication date: 20110330 |