CN101720691A - Seed production method for breeding pinctada martensii - Google Patents
Seed production method for breeding pinctada martensii Download PDFInfo
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- CN101720691A CN101720691A CN200910194337A CN200910194337A CN101720691A CN 101720691 A CN101720691 A CN 101720691A CN 200910194337 A CN200910194337 A CN 200910194337A CN 200910194337 A CN200910194337 A CN 200910194337A CN 101720691 A CN101720691 A CN 101720691A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a seed production method for breeding pinctada martensii, which comprises the following steps: selecting individuals with obvious characteristic of golden yellow shell color from wild or bred colonies as parents to perform colony sex so as to establish basic colonies; performing colony selection on the basic colonies according to the characteristics of shell color and shell weight index; and after the selection of 3 to 5 generations, selecting the colonies in which the proportion of the golden yellow shell individuals can reach 95 percent and the shell weight index is improved by over 20 percent. The method for breeding a new pinctada martensii strain with obvious characteristics of shell color and shell weight index by using the golden yellow shell individuals of the wild or bred colonies as parents has stronger operability and can realize industrialized production.
Description
Technical field
The present invention relates to a kind of producing method for seed of culturing pteria martensii.
Background technology
Pteria martensii Pinctada martensii (Dunker) is one of main economic shellfish of the southern Guangdong of China, Guangxi and three provinces and regions, Hainan.Culturing the pteria martensii main purpose is to cultivate sea water pearls.The pearl that utilizes pteria martensii to cultivate in China have the title of " Nan Zhu ", because of its exquisiteness is thought highly of, smooth as jade Zhu Yuan, extraordinarily beautiful and magnificent and gloss is prolonged has won fame both at home and abroad.
Nineteen sixty-five, China's pteria martensii artificial breeding is achieved success.So far, the existing history of propagating artificially in more than 40 year of pteria martensii.Owing to long-term reasons such as inbreeding, shellfish body serious problems such as diminish sex premature appearred; Many sea areas cultivation density is excessive, and breeding environment worsens, and causes the quality of sea water pearl of our province obviously landslide, famine competitiveness in the international market to occur.Therefore, cultivate suitable China sea area breed pteria martensii improved seeds and seem very important.
Before formulating breeding plan, should establish suitable breeding goal.Culturing the Pinctada martensii purpose is to cultivate the artificial pearl.It is that the outer embrane of piece shell is cut into pieces that the artificial pearl cultivates, and mantle piece and pearl nucleus transplantation is arrived in the pearly shellfish body again.Estimate artificial nucleated pearl qualitative factor and mainly comprise nacre thickness, pearly luster, size and color etc., wherein there is a kind of obvious positive correlation in the shell of nacre thickness, size and color and pearly shellfish shell look heavy and piece shell.For example, Wada etc. (1996) has reported and has utilized the white shell chromaticity of pteria martensii system can cultivate high-quality white pearl as the mantle piece donor.Therefore, the shell look and the shell ponderal index of pinctada should be as the seed selection indexs when the breeding plan of the science of formulation.
Aspect the research of shellfish shell look, China's researcher carries out directive breeding in conjunction with shell look and growth traits to haliotis discus hannai Ino Haliotis discus hannai, bay scallop Argopecten irradians irradians and Ruditapes philippinarum Ruditapes philippinarum, has cultivated the new lines that has obvious shell look and economic characters simultaneously.For example, " gulf, the middle section Red sea scallop " that Zhang Guofan etc. (2005) cultivate, the offspring individual more than 95% is orange shell look, and survival rate improves 15-20%, and growth improves 10-15%, and dressing percentage increases more than 10%, and shell is thick also obviously to be increased.The shell look of pteria martensii cultured population comprises brown, brown, red, yellow and white, and ratio wherein yellow and albino is less.At present, Shang Weijian is in conjunction with the research report of pteria martensii shell look and colony's seed selection.
Summary of the invention
The object of the invention provides a kind of producing method for seed of culturing pteria martensii, selects the tangible individuality of golden yellow shell color characteristic from wild or cultured population and sets up basic population for the parent carries out flock-mate; Carry out colony's seed selection according to shell look and shell ponderal index character pair basic population; For after selecting, the individual ratio of the golden yellow shell look of choosing group can reach 95% through 3-5, and the shell ponderal index improves more than 20%, and this strain has obvious golden yellow shell look and shell ponderal index properties and characteristics simultaneously.
The object of the present invention is achieved like this: a kind of producing method for seed of culturing pteria martensii, it comprises the following steps: that successively (1) basic population makes up: it is significantly individual for the parent to select golden yellow shell color characteristic from wild or cultured population, carries out flock-mate to set up basic population; (2) colony is selected: carry out seed selection according to shell look and shell ponderal index character pair basic population.
Basic population in described (1) makes up, and comprises the following steps: successively
The a parent selects and accelerating
Never comprovincial wild or cultured population selects that golden yellow shell color characteristic is obvious, the full individuality of sexual gland, 1.0-2.0 age, the long 6.0-7.0cm of shell.In indoor accelerating 5-7 days, change water every day once, the sufficient bait of throwing something and feeding promotes that sexual gland further develops into the physiological ripening state.During the accelerating, water temperature 23-25 ℃, salinity 30 ‰.
B induces gamete discharging and insemination
Employing is dried in the shade and pipeline mode induces the parent to discharge gamete, and female and male gametophyte is placed respectively in the Plastic Drum that fills filtering sea.
C carries out artificial insemination with the female and male gametophyte that obtains in the flock-mate mode.
D is hatched with the later stage according to routine techniques and is cultivated.
(2) colony is selected: select from basic population and have obvious golden yellow shell color characteristic proterties, and the shell ponderal index greater than the individuality of the average 15-20% of colony as the parent, selection intensity is 7-10%.Carry out parent's selection and accelerating, induce gamete discharging, artificial insemination and larval culture according to the method for above-mentioned (1).For after selecting, the individual ratio of the golden yellow shell look of choosing group can reach 95% through 3-5, and the shell ponderal index improves more than 20%.
The present invention has following advantage:
Can cultivate the pteria martensii strain that has obvious golden yellow shell look and shell ponderal index properties and characteristics simultaneously by the present invention, method is simple and feasible, can obviously improve pearl quality, can realize industrialization production.
Embodiment
Embodiment 1
Place: the profound extra large shrimp seedling of Zhanjiang crow stone field, time: select 150 golden yellow shell look individualities from the big vast sea area in drift sand port, Zhanjiang and back pteria martensii cultured population in April, 2005,1.5-2.0 age, the long 6.35 ± 1.12cm of the average shell of cultured population.Indoor accelerating is cultivated, and changes water every day once, throw something and feed sufficient chlorella and flat algae.Grow to becoming the physiological ripening state through parent after 7 days.During the accelerating, 24 ℃ of water temperatures, salinity 30 ‰.Discharge gamete through parent behind the 20min that dries in the shade, female and male gametophyte is filtered with 300 mesh sieve thin,tough silk, put into the 30L Plastic Drum respectively, add the 20L seawater simultaneously, in the bucket that fills oogamete, add a spot of mixing sperm then, fully stir evenly and carry out artificial insemination.Carry out incubating oosperm and later stage cultivation according to routine techniques.Between mature stage (1.0 age), colony's golden yellow shell color ratio example reaches 90%.
Embodiment 2
Place: Guangdong, Xuwen, Zhanjiang bank China Group Co.,Ltd, time: in March, 2008, breeding population is carried out the colony's selection of the 3rd generation according to shell look and shell ponderal index target.From golden yellow shell look choosing is F
21000 of random sampling have obvious golden yellow shell color characteristic individuality, select 80 individualities as the parent according to the shell look 1.2 ages, and choosing is F
2Average shell ponderal index is 19.63 ± 2.61, and parental mean shell ponderal index is 21.12 ± 1.34.Indoor accelerating is cultivated, and changes water every day once, throw something and feed sufficient chlorella and flat algae.Grow to becoming the physiological ripening state through parent after 7 days.During the accelerating, 25 ℃ of water temperatures, salinity 30 ‰.Discharge gamete through parent behind the 20min that dries in the shade, female and male gametophyte is filtered with 300 mesh sieve thin,tough silk, put into the 30L Plastic Drum respectively, add the 20L seawater simultaneously, in the bucket that fills oogamete, add a spot of mixing sperm then, fully stir evenly and carry out artificial insemination.Carry out incubating oosperm and later stage cultivation according to routine techniques.During the breed (1.3 age), choosing is F
3Golden yellow shell excitation reaches 96%, and the shell ponderal index is 20.78 ± 3.21, has improved 21.2% than control group.
Claims (1)
1. producing method for seed of culturing pteria martensii, it is characterized in that utilizing wild or basic population is set up in the individual flock-mate of the golden yellow shell look of cultured population, and this basic population selected, obtain to have the pteria martensii new lines of golden yellow shell look and shell ponderal index properties and characteristics simultaneously, can operate according to following steps:
(1) basic population makes up
The a parent selects and accelerating
Never comprovincial wild or cultured population selects that golden yellow shell color characteristic is obviously individual to be the parent, 1.0-2.0 age, the long 6.0-7.0cm of shell.Indoor accelerating 5-7 days, change water every day once, the sufficient bait of throwing something and feeding promotes that sexual gland further develops into the physiological ripening state.During the accelerating, water temperature 23-25 ℃, salinity 30 ‰;
B induces gamete discharging and insemination
Employing is dried in the shade and pipeline mode induces the parent to discharge gamete, female and male gametophyte is placed respectively in the Plastic Drum that fills filtering sea;
C carries out artificial insemination with the female and male gametophyte that obtains in the flock-mate mode;
D is hatched with the later stage according to routine techniques and is cultivated;
(2) colony is selected
Select from basic population and to have obvious golden yellow shell color characteristic proterties, and the shell ponderal index greater than the individuality of the average 15-20% of colony as the parent, selection intensity is 7-10%.Through 3-5 for seed selection after, can cultivate the new lines that has golden yellow shell look and shell ponderal index feature simultaneously.
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| CN200910194337A CN101720691A (en) | 2009-11-24 | 2009-11-24 | Seed production method for breeding pinctada martensii |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102388828A (en) * | 2011-10-09 | 2012-03-28 | 中国科学院南海海洋研究所 | Breeding method for rapid growing line of seawater pearl shells |
| CN102960272A (en) * | 2012-11-09 | 2013-03-13 | 中国水产科学研究院南海水产研究所 | Quantitative character gene pyramiding breeding method for pinctada fucata |
| CN103070095A (en) * | 2012-11-29 | 2013-05-01 | 中国水产科学研究院淡水渔业研究中心 | Fine breed preservation method of genetically improved farmed tilapia form selection |
| CN103975883A (en) * | 2014-05-23 | 2014-08-13 | 广东海洋大学 | Breeding method of pinctada martensi black-shell strain for sea pearl cultivation |
| CN104839068A (en) * | 2015-06-05 | 2015-08-19 | 广东海洋大学 | Selective breeding method for breeding piece shell families of gold pearl layers of pinctada martensi of seawater pearls |
| CN110301384A (en) * | 2019-07-23 | 2019-10-08 | 湖南文理学院 | A kind of nacreous pearl culturing method of mass production |
| CN112586413A (en) * | 2020-12-31 | 2021-04-02 | 广东尊鼎珍珠有限公司 | Golden yellow seawater pearl cultivation method |
| CN113287552A (en) * | 2021-06-29 | 2021-08-24 | 广西海洋研究所有限责任公司 | Method for synchronously preserving wild pinctada martensii |
| CN114342847A (en) * | 2022-01-20 | 2022-04-15 | 中国科学院南海海洋研究所 | Seed production method for improving growth of pinctada martensii with golden yellow shell color |
-
2009
- 2009-11-24 CN CN200910194337A patent/CN101720691A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102388828A (en) * | 2011-10-09 | 2012-03-28 | 中国科学院南海海洋研究所 | Breeding method for rapid growing line of seawater pearl shells |
| CN102960272A (en) * | 2012-11-09 | 2013-03-13 | 中国水产科学研究院南海水产研究所 | Quantitative character gene pyramiding breeding method for pinctada fucata |
| CN102960272B (en) * | 2012-11-09 | 2014-02-19 | 中国水产科学研究院南海水产研究所 | Quantitative character gene pyramiding breeding method for pinctada fucata |
| CN103070095A (en) * | 2012-11-29 | 2013-05-01 | 中国水产科学研究院淡水渔业研究中心 | Fine breed preservation method of genetically improved farmed tilapia form selection |
| CN103975883A (en) * | 2014-05-23 | 2014-08-13 | 广东海洋大学 | Breeding method of pinctada martensi black-shell strain for sea pearl cultivation |
| CN104839068A (en) * | 2015-06-05 | 2015-08-19 | 广东海洋大学 | Selective breeding method for breeding piece shell families of gold pearl layers of pinctada martensi of seawater pearls |
| CN110301384A (en) * | 2019-07-23 | 2019-10-08 | 湖南文理学院 | A kind of nacreous pearl culturing method of mass production |
| CN110301384B (en) * | 2019-07-23 | 2021-07-27 | 湖南文理学院 | Pearl cultivation method for massively produced pearl mussels |
| CN112586413A (en) * | 2020-12-31 | 2021-04-02 | 广东尊鼎珍珠有限公司 | Golden yellow seawater pearl cultivation method |
| CN113287552A (en) * | 2021-06-29 | 2021-08-24 | 广西海洋研究所有限责任公司 | Method for synchronously preserving wild pinctada martensii |
| CN114342847A (en) * | 2022-01-20 | 2022-04-15 | 中国科学院南海海洋研究所 | Seed production method for improving growth of pinctada martensii with golden yellow shell color |
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Open date: 20100609 |