CN102763617B - Artificial breeding method of sikamea oysters - Google Patents

Artificial breeding method of sikamea oysters Download PDF

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Publication number
CN102763617B
CN102763617B CN2012102964746A CN201210296474A CN102763617B CN 102763617 B CN102763617 B CN 102763617B CN 2012102964746 A CN2012102964746 A CN 2012102964746A CN 201210296474 A CN201210296474 A CN 201210296474A CN 102763617 B CN102763617 B CN 102763617B
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China
Prior art keywords
larvae
shellfish
oyster
breeding method
sikamea
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Expired - Fee Related
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CN2012102964746A
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CN102763617A (en
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李琪
王卫军
丛日浩
王许波
孔令锋
杨建敏
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SHANDONG PROVINCE MARINE LIVINGS RESEARCH INST
Ocean University of China
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SHANDONG PROVINCE MARINE LIVINGS RESEARCH INST
Ocean University of China
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses an artificial breeding method of sikamea oysters. The artificial breeding method of the sikamea oysters is characterized by including the following steps: A, adult sikamea oyster Pacific oysters are led in from America and bred in an indoor seawater nursery, and Pacific oyster maturing is performed; B, by means of an oxytocin method, the Pacific oyster is induced to discharge sperm and eggs, and the fertilized eggs perform larva hatch; C, when hatched larvae grow into larvae of D, optimization is performed, and the larvae are arranged in a cultivation pool to be cultivated; and E, when the larvae is cultivated to 350-380 mum and eye spots appear, scallop shell attached bases are put in to adhering seedlings; when the larvae is cultivated to 600 mum, the larvae are placed in the middle of an outdoor sedimentation basin to be cultivated; and when juvenile mollusks grow to 5mm, the juvenile mollusks are placed in a natural sea area to be cultivated into adult mollusks. The artificial breeding method of sikamea oysters is established through the steps of Pacific oyster maturing, sperm and egg discharging through induction, larva cultivation, artificial seeding, middle cultivation of the juvenile mollusks, maturing at the natural sea area and the like, and can cultivate sikamea oyster seedlings in a large scale.

Description

The artificial breeding method of a kind of Kumamoto oyster
Technical field
The invention belongs to the artificial breeding method of a kind of shellfish, particularly relate to the artificial breeding method of a kind of Kumamoto oyster.
Background technology
Kumamoto oyster (Crassostrea sikamea) originates in East Asia, is introduced into U.S.West Coast the forties in 20th century.The individual mostly oval of Kumamoto oyster, and less, generally can not surpass 10cm, growth 2-3 reaches commercial specification; Left shell is the set shell, inwardly gets deeply stuck in, and tool is vertical rib significantly, is devils claw, and right shell is flat; Kumamoto oyster chitin is hard, and meat is fresh and sweet, and price is a times of long oyster (Crassostrea gigas) in the international market, has very high economic worth.
The present invention becomes shellfish by introducing the Kumamoto oyster from the U.S., reproductive habit according to the Kumamoto oyster, by incubation, induce discharging smart ovum, larvae cultivation, artificial seeding, juvenile mollusk intermediate rearing, natural waters the step such as to form, set up the artificial breeding method of Kumamoto oyster, can cultivate on a large scale Kumamoto oyster seed.
Summary of the invention
The artificial breeding method who the purpose of this invention is to provide a kind of Kumamoto oyster, the present invention is significant for the new breed variety of the artificial breeding of carrying out the Kumamoto oyster and cultivation, increase.
The artificial breeding method of a kind of Kumamoto oyster is characterized in that: comprise following steps:
A, introduce adult Kumamoto oyster parent shellfish from the U.S., and with its cultivation in indoor seawater seed rearing field, carry out incubation;
B, the induced spawning method by lowering the temperature and heat up first induce close shellfish to discharge smart ovum, place specific hatchery to carry out the larva hatching fertilized egg;
C, the larvae development that hatches be during to D shape larva, selects excellently, and D shape larva placed cultivates the pond and cultivate;
When E, larvae cultivation eyespot occurs to 350-380 μ m, throw in the scallop shell adherance and carry out spatfall; When larvae cultivation to 600 μ m is above, place outdoor sedimentation basin to carry out intermediate rearing; Juvenile mollusk grows to 5mm when above, places natural waters to become shellfish to form.
Say further:
It is individual to adopt the Kumamoto oyster that catches 2-3 age, the high 6-10cm of shell from U.S.'s natural waters, and as the close shellfish of breeding, the water temperature of described indoor seawater seed rearing field is controlled at 26-29 ℃.
Further say:
The method of described incubation is to place indoor water temperature 28-29 ℃, the indoor seawater seed rearing field of 10-20m3 to cultivate throw something and feed every day chrysophyceae, chlorella, flat algae and spirulina Kumamoto oyster parent shellfish.
After the described close shellfish gonadal maturation, at first in 20 ℃ low temperature seawater, placed 3-5 hour, closeer shellfish is placed 28 ℃ high temperature seawater to induce close shellfish to discharge smart ovum; When determining around the ovum sperm quantity 5-10 by microexamination, male is taken out; Place the hatchery of water temperature 28-29 ℃ 10-20m3 to carry out the larva hatching fertilized egg.
Further say:
Described larvae development is during to D shape larva, and it is excellent to use 350 mesh sieve thin,tough silk to select, and places the cultivation pond of 10-20m3 to cultivate D shape larva.
Owing to adopted technique scheme, the present invention is according to the reproductive habit of Kumamoto oyster, by incubation, induce discharging smart ovum, larvae cultivation, artificial seeding, juvenile mollusk intermediate rearing, natural waters the step such as to form, set up the artificial breeding method of Kumamoto oyster, can cultivate on a large scale Kumamoto oyster seed.
Embodiment
The invention will be further described below in conjunction with example:
1, on June 26th, 2012, from U.S.'s natural waters, adopt catch 2-3 age, the high 6-10cm of shell 120 of individualities as the close shellfishes of breeding, this moment, the nursery water temperature was 26.5 ℃.
2, incubation: place the water body of the 18m3 of 28 ℃ of indoor water temperatures to cultivate close shellfish, throw something and feed every day chrysophyceae, chlorella, flat algae and spirulina.
3, induced spawning and incubating oosperm: dissect 3-5 close shellfish, examine under a microscope the gonad development situation.When ovum and spermioteleosis, close shellfish at first is placed in 20 ℃ the low temperature seawater 4 hours, closeer shellfish is placed 28 ℃ high temperature seawater to induce close shellfish to discharge smart ovum; When determining around the ovum sperm quantity 5-10 by microexamination, male is taken out; Place the hatchery of the 10-20m3 of 28 ℃ of water temperatures to carry out the larva hatching fertilized egg.
4, select children and larvae cultivation: when larvae development during to D shape larva, use 350 mesh sieve thin,tough silk to select the children, and D shape larva is placed the cultivation pond of 20m3, density is 8/ml; D shape larva is cultivated density in early days less than 10/ml, the chrysophyceae of throwing something and feeding; Shell pushes up larva early metaphase density less than 5/ml, throw something and feed chrysophyceae and chlorella; In the shell top larva later stage, culture density is not more than 1/ml, mixes throw something and feed chlorella and flat algae; Day is changed waterside several 3 times, and a day quantity of exchanged water is 2 full doses, day bait throwing in number of times 3 times; Cultivate salinity 28-30, water temperature 26-29 ℃.
5, spatfall: eyespot appears in larvae cultivation to 350 μ m, and when the eyebot larvae rate reaches 25%, throws in the scallop shell adherance and carry out spatfall, during the settlement and metamorphosis, strengthens aeration quantity, quantity of exchanged water and daily ration, feeding quantity;
6, juvenile mollusk is cultivated: larva adheres to rear cultivation to 600 μ m when above, places outdoor sedimentation basin intermediate rearing.Juvenile mollusk grows to 5mm when above, adopts cylinder mould buoyant raft hanging culture mode, places natural waters to form.
Should be noted that at last; above content is only in order to illustrate technical scheme of the present invention; but not limiting the scope of the invention; the simple modification that those of ordinary skill in the art carries out technical scheme of the present invention or be equal to replacement does not all break away from essence and the scope of technical solution of the present invention.

Claims (4)

1. the artificial breeding method of a Kumamoto oyster is characterized in that: comprise following steps:
A, introduce adult Kumamoto oyster parent shellfish from the U.S., adult Kumamoto oyster parent shellfish is that to adopt the Kumamoto oyster that catches 2-3 age, the high 6-10cm of shell from U.S.'s natural waters individual, as the close shellfish of breeding, and with its cultivation in indoor seawater seed rearing field, carry out incubation, and the water temperature of described indoor seawater seed rearing field is controlled at 26-29 ℃;
B, the induced spawning method by lowering the temperature and heat up first induce close shellfish to discharge smart ovum, and fertilized egg is carried out the larva hatching in the hatchery;
C, the larvae development that hatches be during to D shape larva, selects excellently, and D shape larva placed cultivates the pond and cultivate;
When E, larvae cultivation eyespot occurs to 350-380 μ m, throw in the scallop shell adherance and carry out spatfall; When larvae cultivation to 600 μ m is above, place outdoor sedimentation basin to carry out intermediate rearing; Juvenile mollusk grows to 5mm when above, places natural waters to become shellfish to form.
2. the artificial breeding method of Kumamoto oyster according to claim 1, it is characterized in that: the method for described incubation is to place indoor water temperature 28-29 ℃, the indoor seawater seed rearing field of 10-20m3 to cultivate throw something and feed every day chrysophyceae, chlorella, flat algae and spirulina Kumamoto oyster parent shellfish.
3. the artificial breeding method of Kumamoto oyster according to claim 1, it is characterized in that: after the described close shellfish gonadal maturation, at first close shellfish is placed in 20 ℃ the low temperature seawater 3-5 hour, closeer shellfish is placed 28 ℃ high temperature seawater to induce the smart ovum of discharging; When determining around the ovum sperm quantity 5-10 by microexamination, male is taken out; Place the hatchery of water temperature 28-29 ℃ 10-20m3 to carry out the larva hatching fertilized egg.
4. the artificial breeding method of Kumamoto oyster according to claim 1 is characterized in that: described larvae development is during to D shape larva, and it is excellent to use 350 mesh sieve thin,tough silk to select, and places the cultivation pond of 10-20m3 to cultivate D shape larva.
CN2012102964746A 2012-08-20 2012-08-20 Artificial breeding method of sikamea oysters Expired - Fee Related CN102763617B (en)

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CN103798168A (en) * 2014-01-24 2014-05-21 中国科学院南海海洋研究所 Novel Indoor large-scale artificial breeding method suitable for south China coastal salinity tapering type crassostrea sikamea

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CN104012432A (en) * 2014-04-25 2014-09-03 中国海洋大学 Method suitable for artificial breeding of CrassOstrea hongkongensis in sea area in north
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