CN105475185B - A kind of rock oyster incubation is cultivated and the method for spawning - Google Patents
A kind of rock oyster incubation is cultivated and the method for spawning Download PDFInfo
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- CN105475185B CN105475185B CN201510841936.1A CN201510841936A CN105475185B CN 105475185 B CN105475185 B CN 105475185B CN 201510841936 A CN201510841936 A CN 201510841936A CN 105475185 B CN105475185 B CN 105475185B
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- oyster
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- rock oyster
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
A kind of method cultivated and laid eggs the invention provides rock oyster incubation.Rock oyster parent shellfish is selected first is positioned over progress accelerating cultivation in nursery pond；Rock oyster Micro Algae bait is fed, and ripe is reached to the close shellfish sexual gland of rock oyster progress accelerating cultivation by progressively heating up；It is up to after the ripe rock oyster parent shellfish of sexual gland dries in the shade, is put into spawning pond；The female shellfish of Pacific oyster of maturation is picked out by microscopy, and dissects taking-up ovum, and ovum is filtered with screen cloth again, fragment of tissue and Pacific oyster ovum is removed, obtains tissue filter liquid；The tissue filter liquid is added in above-mentioned spawning pond, the spawning of induction rock oyster parent shellfish, row's essence；Rock oyster parent shellfish is finally removed, obtains the embryonated egg of artificial breeding.The present invention can solve the problem that rock oyster room heat source incubation cultivates the problem of ripe difficult, spawning is difficult, and spawning is fast, and archiblast is good, meets rock oyster artificial breeding production requirement, be provided the foundation for the production of rock oyster in scale.
Description
Technical field
The present invention relates to a kind of method of rock oyster artificial breeding, cultivated more particularly to a kind of rock oyster incubation and
The method of spawning.
Background technology
Rock oyster (Crassostrea nippona) it is under the jurisdiction of Mollusca, lamellibranchiata, Ostreidae, huge oyster category, it is one
The important economic bivalve shellfish of marine products of kind.Rock oyster is mainly distributed on the ground such as Japan, Australia, and it has, and body is big, growth is fast, production
The features such as amount is high, dressing percentage is high and high temperature resistant, resistance against diseases are strong, strong environmental adaptability, it is the oyster culture of recent development
Excellent species.The season that rock oyster suitably eats is in summer, and summer oyster is called in Japan, and the Pacific Ocean that the country generally cultivates is male
The season that oyster suitably eats in the winter time, because Pacific oyster was bred in spring, glycogen content in summer Pacific oyster body
Relatively low, poor taste, and be not suitable for eating.It is difficult to provide high-quality edible oyster always on current domestic summer fish market.
At present, because rock oyster has the problem of incubation cultivates difficulty and difficult spawning during artificial breeding,
The rock oyster culture of Japan relies primarily on collection nature rock oyster seed and produced, and nature rock oyster seed is time-consuming takes for collection
Power, collection capacity are limited by a variety of natural conditions, are only hardly formed large-scale cultivation by gathering nature rock oyster seed, also
It is difficult to meet summer market to the great demand of high-quality oyster.
China introduced the time of rock oyster less than 10 years, and the research of artificial breeding technique is still in experimental stage, rock oyster
Artificial breeding is mainly carried out using the artificial breeding technique route and technique of Pacific oyster, due to rock oyster and China Pacific Ocean
Oyster is that reproductive biology is two entirely different oyster kinds, and it is male that the artificial breeding technique of Pacific oyster is not appropriate for rock
The cultivation of oyster, rock oyster parent shellfish is ripe difficult, and egg laying amount is few, can not meet the level of productive receivers completely.As cultivation is advised
The expansion of mould, in the rock oyster artificial breeding technique research accelerating of rock oyster parent shellfish more and more to the demand of rock oyster seed
Cultivate and spawning is the current technical barrier for urgently needing to solve.
The content of the invention
To solve the above problems, a kind of method cultivated and laid eggs it is an object of the invention to provide rock oyster incubation,
This method improves the egg laying amount of close shellfish, overcomes existing rock male by carrying out being directed to Journal of Sex Research to the gonad maturity accelerating of rock oyster parent shellfish
The shortcomings that oyster artificial breeding technique.
To reach above-mentioned purpose, the concrete technical scheme that the present invention takes is：
A kind of rock oyster incubation is cultivated and the method for spawning, it is characterized in that comprising the following steps：
（1）Rock oyster parent shellfish is selected first is positioned over the accelerating cultivation that next step is carried out in nursery pond；
（2）In the accelerating nurturing period, rock oyster Micro Algae bait is fed, and accelerating is carried out to rock oyster by progressively heating up
Cultivate and cause close shellfish sexual gland to reach ripe；
（3）It is up to after the ripe rock oyster parent shellfish of sexual gland dries in the shade, is put into spawning pond；
（4）The female shellfish of Pacific oyster of maturation is picked out by microscopy, dissection is carried out to it and takes out ovum, by taking-up too
Put down foreign oyster ovum to be filtered with screen cloth again, remove fragment of tissue and Pacific oyster ovum, obtain tissue filter liquid；
（5）By step（4）Obtained tissue filter liquid is added to step（3）In spawning pond in, to induce rock male
The spawning of oyster parent shellfish, row's essence；
（6）Rock oyster parent shellfish lays eggs to required embryonated egg, removes rock oyster parent shellfish, obtains the fertilization of artificial breeding
Ovum.
Add and induce rock oyster after 20-30 minutes after the tissue fluid and start essence of laying eggs, arrange, rock oyster lays eggs to artificial
After embryonated egg needed for nursery, rock oyster parent shellfish is removed, finally a point pond is hatched under 30-32 DEG C of water temperature, through selecting excellent, larva to train
Educate, collect seedling, cultivate the seed required for rock oyster culture.
The step（2）In conditionting culture cultivate specific temperature-rising method be：Rock oyster parent shellfish enters water temperature for 10 DEG C,
Heated up 0.5-1.0 DEG C by changing water daily, it is stable at a temperature of 30-35 DEG C to cultivate after being warming up to 30 DEG C, accumulate temperature in day
Parent's shellfish sexual gland reaches ripe when degree reaches 500-1000 DEG C.
The step（1）In to select size 10-15cm rock oyster be close shellfish, by 50-60/m3It is placed in nursery pond
Row conditionting culture is cultivated.
The step（2）In feed the 6-8 unicellular alga including diatom, chrysophyceae and flat algae daily, feed day
Measure as (10-30) × 104Individual/milliliter days.
The step（3）Middle rock oyster parent shellfish is dried in the shade 5-6 hours, and temperature is maintained at 30-35 DEG C in spawning pond.
The step（4）Middle Pacific oyster ovum is filtered using mesh for the bolting silk net of 20-25 microns.
The step（6）Middle addition 5-10 rises tissue filter liquid to step（3）In spawning pond in.
The present invention has the advantage that：The present invention is directed to the growth characteristics of rock oyster, in conditionting culture cultivating process, gradually
30-35 DEG C is warming up to, this temperature range is not appropriate for the accelerating culture of other kind oysters, the heating model of other species oysters
Enclose typically not over 25 DEG C, otherwise the sexual gland maturation of close shellfish can be had an impact.But rock oyster during accelerating, it is necessary to
It is warming up to 30-35 DEG C and can be only achieved preferable accelerating effect；In addition, the present invention uses the tissue filter liquid of Pacific oyster ovum
Close shellfish sexually matured to rock oyster induces, so as to output substantial amounts of ovum and sperm.The present invention can solve the problem that rock oyster
Room heat source incubation cultivates ripe difficulty, lays eggs the problem of difficult, and spawning is fast, and archiblast is good, and it is artificial to meet rock oyster
Nursery production requirement, provided the foundation for the production of rock oyster in scale.
Embodiment
Below by way of specific embodiment, the present invention is further described.
The rock oyster incubation of the present embodiment is cultivated and the method for spawning, comprises the following steps：
（1）The rock oyster for selecting size 10-15cm first is close shellfish, by 50-60/m3It is placed in nursery pond and carries out down
The conditionting culture of one step is cultivated；
（2）In the accelerating nurturing period, rock oyster bait is fed, feeds 6-8 times daily including diatom, chrysophyceae and flat algae
Unicellular alga, day feeding volume (10-30) × 104Individual/milliliter days；And accelerating training is carried out to rock oyster by progressively heating up
Educate so that close shellfish sexual gland reaches ripe, specially rock oyster parent shellfish enters water temperature for 10 DEG C, daily by changing water heating 0.5-1.0
DEG C, stable at a temperature of 30-35 DEG C to cultivate after being warming up to 30 DEG C, parent's shellfish property when day accumulation temperature reaches 500-1000 DEG C
Gland reaches ripe；
（3）It is up to the ripe rock oyster parent shellfish of sexual gland to dry in the shade 5-6 hours, is put into spawning pond, and temperature in spawning pond
It is maintained at 30-35 DEG C；
（4）The female shellfish of Pacific oyster of maturation is picked out by microscopy, dissection is carried out to it and takes out ovum, by taking-up too
Put down foreign oyster ovum to be filtered with screen cloth again, remove fragment of tissue and Pacific oyster ovum, obtain tissue filter liquid；
（5）By step（4）Obtained 5-10 rises tissue filter liquid and is added to step（3）In spawning pond in, add institute
State and induce the spawning of rock oyster parent shellfish, row's essence after tissue fluid after 20-30 minutes；
（6）Rock oyster parent shellfish lays eggs to required embryonated egg, removes rock oyster parent shellfish, obtains the fertilization of artificial breeding
Ovum；
（7）Finally by obtained embryonated egg, a point pond is hatched under 30-32 DEG C of water temperature, through selecting excellent, larvae cultivation, collecting seedling, training
Bring out the high-quality seed required for rock oyster culture.
The above embodiments merely illustrate the technical concept and features of the present invention, and the protection model of the present invention can not be limited with this
Enclose.All equivalent transformations done according to spirit of the invention or modification, it should all cover within the scope of the present invention.
Claims (7)
1. a kind of rock oyster incubation is cultivated and the method for spawning, it is characterized in that comprising the following steps：
（1）Rock oyster parent shellfish is selected first is positioned over the accelerating cultivation that next step is carried out in nursery pond；
（2）In the accelerating nurturing period, rock oyster Micro Algae bait is fed, and accelerating cultivation is carried out to rock oyster by progressively heating up
So that close shellfish sexual gland reaches ripe；
（3）It is up to after the ripe rock oyster parent shellfish of sexual gland dries in the shade, is put into spawning pond；
（4）The female shellfish of Pacific oyster of maturation is picked out by microscopy, dissection is carried out to it and takes out ovum, by the Pacific Ocean of taking-up
Oyster ovum is filtered with screen cloth again, is removed fragment of tissue and Pacific oyster ovum, is obtained tissue filter liquid；
（5）By step（4）Obtained tissue filter liquid is added to step（3）In spawning pond in, to induce rock oyster close
Shellfish spawning, row's essence；
（6）Rock oyster parent shellfish lays eggs to required embryonated egg, removes rock oyster parent shellfish, obtains the embryonated egg of artificial breeding.
2. rock oyster incubation as claimed in claim 1 is cultivated and the method for spawning, it is characterized in that the step（2）In
Conditionting culture cultivate specific temperature-rising method be：Rock oyster parent shellfish enters water temperature for 10 DEG C, daily by changing water heating 0.5-1.0
DEG C, stable at a temperature of 30-35 DEG C to cultivate after being warming up to 30 DEG C, parent's shellfish property when day accumulation temperature reaches 500-1000 DEG C
Gland reaches ripe.
3. rock oyster incubation as claimed in claim 1 is cultivated and the method for spawning, it is characterized in that the step（1）In choose
The rock oyster for selecting size 10-15cm is close shellfish, by 50-60/m3It is placed on progress conditionting culture cultivation in nursery pond.
4. rock oyster incubation as claimed in claim 1 is cultivated and the method for spawning, it is characterized in that the step（2）In it is every
It feeds the 6-8 unicellular alga including diatom, chrysophyceae and flat algae, day feeding volume (10-30) × 104Individual/milliliter
My god.
5. rock oyster incubation as claimed in claim 1 is cultivated and the method for spawning, it is characterized in that the step（3）Middle rock
Oyster parent shellfish is dried in the shade 5-6 hours, and temperature is maintained at 30-35 DEG C in spawning pond.
6. rock oyster incubation as claimed in claim 1 is cultivated and the method for spawning, it is characterized in that the step（4）In too
Foreign oyster ovum is put down to be filtered for the bolting silk net of 20-25 microns using mesh.
7. rock oyster incubation as claimed in claim 1 is cultivated and the method for spawning, it is characterized in that the step（6）In plus
Enter 5-10 and rise tissue filter liquid to step（3）In spawning pond in.
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719185B (en) * | 2017-01-05 | 2020-05-19 | 中国海洋大学 | Method for ecologically breeding crassostrea gigas seedlings by utilizing high-temperature period |
| CN113678764B (en) * | 2020-12-31 | 2023-08-04 | 青岛前沿海洋种业有限公司 | Method for producing tetraploid oyster and interspecific hybridization triploid oyster |
| CN114600806B (en) * | 2022-03-31 | 2023-01-24 | 广东海洋大学 | Ripening promoting method for cultivating Xishi lingual parent shellfish by using pond algae |
Citations (5)
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|---|---|---|---|---|
| FR2763477B1 (en) * | 1997-05-23 | 1999-08-20 | Sarl Roger Bertrand | APPARATUS FOR COLLECTING OYSTER OYSTERS FROM COLLECTION CUPS |
| CN102100197A (en) * | 2010-12-20 | 2011-06-22 | 山东海益宝水产股份有限公司 | Method for breading triploid monomer oysters |
| CN102742532A (en) * | 2012-07-31 | 2012-10-24 | 中国海洋大学 | Cultivation method of novel strain of crassostrea gigas with pure purple left shells |
| CN102763617A (en) * | 2012-08-20 | 2012-11-07 | 中国海洋大学 | Artificial breeding method of sikamea oysters |
| CN103858803A (en) * | 2014-03-28 | 2014-06-18 | 中国海洋大学 | Method for breeding new golden-shell crassostrea gigas strain |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH114635A (en) * | 1997-06-16 | 1999-01-12 | Kyowa Hakko Kogyo Co Ltd | Treatment after nucleus insert operation for cultured pearl oyster and chemical dipping agent therefor |
| JP3372213B2 (en) * | 1998-08-24 | 2003-01-27 | 株式会社日本ゲルマニウム研究所 | Pearl production method |
-
2015
- 2015-11-28 CN CN201510841936.1A patent/CN105475185B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2763477B1 (en) * | 1997-05-23 | 1999-08-20 | Sarl Roger Bertrand | APPARATUS FOR COLLECTING OYSTER OYSTERS FROM COLLECTION CUPS |
| CN102100197A (en) * | 2010-12-20 | 2011-06-22 | 山东海益宝水产股份有限公司 | Method for breading triploid monomer oysters |
| CN102742532A (en) * | 2012-07-31 | 2012-10-24 | 中国海洋大学 | Cultivation method of novel strain of crassostrea gigas with pure purple left shells |
| CN102763617A (en) * | 2012-08-20 | 2012-11-07 | 中国海洋大学 | Artificial breeding method of sikamea oysters |
| CN103858803A (en) * | 2014-03-28 | 2014-06-18 | 中国海洋大学 | Method for breeding new golden-shell crassostrea gigas strain |
Non-Patent Citations (1)
| Title |
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| 岩牡蛎人工育苗技术的研究;苏延明等;《大连水产学院学报》;20090515;第24卷(第S1期);第160-162页 * |
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