CN102823414A - Non-tube cutting seedling method of tissue subcultured buds of zenia - Google Patents
Non-tube cutting seedling method of tissue subcultured buds of zenia Download PDFInfo
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- CN102823414A CN102823414A CN2012103459481A CN201210345948A CN102823414A CN 102823414 A CN102823414 A CN 102823414A CN 2012103459481 A CN2012103459481 A CN 2012103459481A CN 201210345948 A CN201210345948 A CN 201210345948A CN 102823414 A CN102823414 A CN 102823414A
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Abstract
The invention discloses a non-tube cutting seedling method of tissue subcultured buds of zenia. The two steps of non-tube rooting of tissue subcultured buds and cutting seedling are combined into one. The tissue subcultured buds of zenia are bred by a conventional method, and then the tissue subcultured buds of zenia are selected as cuttings and are treated by a root promoter and cut into a red subsoil matrix, and seedlings are cultured to obtain forestation wood seedlings for planting. According to the method, the tissue subcultured buds of zenia are directly used as the cuttings for non-tube cutting, the breeding speed is fast, and seasonal and geographical restrictions do not exist; and meanwhile, the two steps of non-tube rooting and cutting seedling are combined into one, the method is simple, the operation is easy, rooting starts after cutting culture for 15 to 20 days, the seedling survival rate is high, and the rooting survival rate is 85% to 95%. The method overcomes the defects of the traditional zenia asexual reproduction method, the seedling cycle is shortened, the seedling survival rate is improved, the seedling cost is reduced, and the method is favorable for accelerating the development of asexual seedling of zenia.
Description
Technical field
The invention belongs to plant Fast Asexual Propagation Technique field, relate to subculture bud seedling-cultivating method, especially relate to a kind of outer cuttage and seedling culture method of beans group training subculture bud bottle.
Background technology
Appointing beans (Zeniainsignis) to have another name called wing pod wood, is the Caesalpiniaceae deciduous tree, is China's endemic tree, and is rare seeds.Appoint beans to have characteristics such as growth is rapid, rudiment power is strong, the root system penetration power is strong, adaptability is strong; (district) economized in Guangxi, Guangdong, Yunnan, Hunan and the Guizhou etc. that are distributed between middle subtropical zone and the south subtropics; Be an important species during southwest and South China's ecological public welfare forests are built, and become one of first-selected indigenous tree species of stone matter planting out, vegetation restoration and reconstruction.
At present, appoint the modes of reproduction of beans to mainly contain three kinds of seminal propagation, cottage propagation and tissue culture propagations.Seminal propagation belongs to sexual propagation, and heredity is unstable, and the mass discrepancy of cultivating nursery stock is bigger, is unfavorable for the popularization of breeding.Cottage propagation and tissue culture propagation belong to vegetative propagation, can keep the merit of breeding, are the wider modess of reproduction of present range of application.
Cottage propagation receives seasonal effect big, be chosen at the beginning of last month of spring in winter, and it is better that the fringe bar does not carry out cuttage before the rudiment as yet; Cuttage this moment helps obtaining higher root survival; After the cuttage 40～50 days begin to take root [1], rootage duration is longer, and the old branch that requires simultaneously to adopt life in 1～2 year is for transplanting a cutting better; If adopt living then spray (terminal bud) to transplant a cutting, being difficult to take root becomes to live [1].Therefore, for obtaining the old branch of suitable cuttage, cutting orchard will could satisfy through 3 years at least adopts the fringe requirement, and the breeding cycle is long, and reproduction coefficient is not high, and cuttage receives the restriction in season again, the popularization opportunity of delay breeding.
Tissue culture propagation receives the influence of season and region little; The successive transfer culture growth rate is fast in the bottle, and the subculture blastogenesis is grown soon, should be taken root, should sprout, under appropriate condition; The subculture bud is cultivated [2] [3] of can taking root about 10 days in root media, be asexual reproduction method preferably.But traditional tissue culture propagation will pass through the step of culture of rootage behind successive transfer culture; Let group train in the medium of subculture bud in bottle and take root, and then transplant to matrix complex procedures; The strict sterile working of culture of rootage could guarantee the quality of growing seedlings in the bottle; And cost is higher relatively, transplants in addition and cultivates, and more need consume more manpower and materials.For appointing the seeds that this growth of beans is rapid, be prone to take root, its group training asexual reproduction method still has improved space.
List of references
[1]. Pan Yuefang, Hao Haikun, Cao Yanyun, thorough company, Huang Zhiling, land state leads, and appoints the test of beans cuttage and seedling culture, forestry science and technology exploitation 2006,20 (6): 65～66
[2]. Yuan Deyi, Zou Feng, He Xiaoyong, handsome ripple, Long Hongxu, the research of wing pod wood group culturation rapid propagating technology, Sino-South African Forestry University of Science and Technology's journal, 2010.30 (6): 60～63
[3]. Qiu Yunliang, the research of wing pod wood test-tube plantlet quick propagating technology, forestry science and technology exploitation, 2002,16 (6): 14～16
Summary of the invention
The object of the invention: in order to overcome existing deficiency of appointing the beans asexual reproduction method, improve reproduction speed, shorten growing-seedling period, the raising survival rate of seedling also reduces cost, and a kind of outer cuttage and seedling culture method of beans group training subculture bud bottle is provided.
Technical scheme of the present invention is: utilize the characteristics that the blastogenesis of beans group training subculture is long soon, should take root, should sprout of appointing; With tissue culture mode breeding subculture bud; Select for use the subculture bud as the fringe bar after rooting promoter is handled; Directly cuttage is being cultivated a beans fast seedling-cultivating method that forms whole plant through growing seedlings to matrix.
The present invention is achieved in that
A kind of outer cuttage and seedling culture method of beans group training subculture bud bottle; Comprise and appoint the cultivation of beans group training subculture bud, matrix preparation, cuttage, grow seedlings and go out 5 steps in garden, it is characterized in that: the method that employing group training subculture bud outside sprout-cultivating-bottle and two steps of cuttage and seedling culture unite two into one, appoint beans group training subculture bud with the conventional method tissue culture propagation earlier; Choose again and appoint beans group training subculture bud as inserting fringe; In rooting promoter processing and cuttage to red heart soil matrix matter, to grow seedlings and cultivate the wooden seedling that obtains afforesting, its operating procedure is following:
(1) group training subculture bud is cultivated: the spray that has axillalry bud of selecting the peripheral middle and upper part of healthy and strong disease-free beans plant tree crown to give birth to then is an explant; Sterilization is seeded to aseptic culture medium and carries out the bud inducing culture; After obtaining aseptic bud, aseptic bud transferred to carry out enrichment culture on the subculture medium;
(2) matrix is prepared: select red heart soil as matrix; Pulverize the back with pulverizer and cross 80～100 mesh sieves; In the plastic cup of the interior 10cm deeply of the 7 * 9cm that packs into; Place on the bed of growing seedlings, with disinfecting solution of potassium permanganate 24～36h of 3g/L～5g/L, clear water drench matrix and cleaning medicine in sterilization back is subsequent use before the cuttage;
(3) cuttage: the subculture bud of clip robust growth is as the fringe bar; After fringe bar shearing end handled with rooting promoter, vertically insert in the matrix of plastic cup, insertion depth accounts for 1/4～1/3 of whole fringe bar; With the clear water matrix of drenching, be May September to next year then in cuttage season after the cuttage;
(4) grow seedlings: the high hut of preserving moisture of 50～70cm is built with the white plastic film in the bed top of growing seedlings, and covers the shading screen of degree of shading 75%～85% again, presses conventional method trickle, weeding, fertilising and the extermination of disease and insect pest during the cultivation cuttage seeding;
(5) go out the garden: more than the height of seedling 50cm, no damage by disease and insect goes out garden afforestation.
The described fringe bar of above step (3) is a subculture bud of selecting height >=5cm, the flush away medium, and joint is sheared at the 3mm place down endways.
It is that fringe bar shearing end is immersed in the MS liquid nutrient medium that contains 0.3mg/L～0.5mg/L ABT6 that the described rooting promoter of above step (3) is handled; Soak part and account for 1/5～1/4 of whole fringe bar length, flushing with clean water is handled and is used for cuttage behind immersion treatment 40～60min.
The present invention's beneficial effect and marked improvement compared with prior art:
(1) method utilization of the present invention appoints beans group training subculture bud should take root, should sprout; And the fast characteristics of growing are to insert fringe to appoint beans group training subculture bud, in slotting fringe cuttage to the red heart soil matrix matter after rooting promoter is handled; Form whole plant under in the hut of building, cultivating; Cuttage is cultivated after 15～20 days and is begun to take root, and root survival is high, reaches 85～95%.
(2) method of the present invention directly will appoint beans group training bud to carry out the outer cuttage of bottle as inserting fringe; With tissue cultivating seedling take root and transplanting is incorporated in the step, need in bottle, not being cultured to takes root again transplants, and compares and traditional group training asexual reproduction method; Further shortened seedling raise period; Simplified operation simultaneously, reduced cost, helped to realize in a short time that large-scale cultivation appoints the beans tissue cultivating seedling.
(3) method of the present invention is chosen and is appointed beans group training subculture bud for inserting fringe; Fringe is inserted in the method vegetative propagation of employing group training, cultivates the seedling-cultivating method of inserting fringe than traditional cutting orchard, takes up an area of few; Not limited by season and region; Reproduction speed improves greatly, and cultivates the shortening cycle, realizes breeding fast, scale cultivation.
(4) use in the cuttage contain 0.3mg/L～0.5mg/L ABT6 the MS liquid nutrient medium as rooting promoter, fringe bar immersion treatment 40～60min, long soak time can be strengthened the rooting effect of rooting promoter.And than the solvent of traditional water as rooting promoter; Select the solvent of MS liquid nutrient medium in this method for use as rooting promoter; Can avoid that the fringe bar receives the influence of organizing inside and outside ion concentration imbalance to cause in long immersion treatment, help the fringe bar after processing, still to keep good state to be beneficial to cuttage.
Embodiment
Below in conjunction with embodiment, the outer cuttage and seedling culture method of a kind of beans group training subculture bud bottle of the present invention is further described.
Embodiment 1:
The spray that has axillalry bud of selecting the peripheral middle and upper part of healthy and strong disease-free beans plant tree crown to give birth to then is an explant, and sterilization is seeded to aseptic culture medium and carries out the bud inducing culture, obtain aseptic bud after, aseptic bud transferred to carries out shoot proliferation on the subculture medium and cultivate.When October, carry out cuttage, select red heart soil as matrix, pulverizer is pulverized the back and is crossed 80～90 mesh sieves; In the plastic cup of the interior 10cm deeply of the 7 * 9cm that packs into; Place on the bed of growing seedlings, before the cuttage with the disinfecting solution of potassium permanganate 24h of 3g/L, sterilization back clear water drench matrix and cleaning medicine; Select robust growth, the subculture bud of height >=5cm is as the fringe bar, behind the flush away medium; Joint is sheared at the 3mm place down endways, and fringe bar end is immersed in the MS liquid nutrient medium that contains 0.3mg/L ABT6, soaks part and accounts for 1/5～1/4 of whole fringe bar length; Flushing with clean water behind the immersion treatment 50min, in the vertical matrix cup that inserts after disinfecting, insertion depth accounts for 1/4～1/3 of whole fringe bar; After cuttage cuttage on the same day finishes, the clear water matrix of drenching, the high hut of preserving moisture of 60cm is built with the white plastic film in the top; Cover the shading screen of degree of shading 75%～85% again, temperature of shed is controlled at 15 ℃～35 ℃, and humidity is controlled at 80%～90%; In time trickle keeps the skin wet, and temperature is too high, and to raise film aeration-cooling.After growing seedlings 20 days, but raise film ventilation 60min every day.After growing seedlings 20 days, cuttage seeding root survival 87%.Cuttage seeding continues timely trickle between culture period, weeding, fertilising and the attention extermination of disease and insect pest, and more than the height of seedling 50cm, no damage by disease and insect can go out garden afforestation.
Embodiment 2:
The spray that has axillalry bud of selecting the peripheral middle and upper part of healthy and strong disease-free beans plant tree crown to give birth to then is an explant, and sterilization is seeded to aseptic culture medium and carries out the bud inducing culture, obtain aseptic bud after, aseptic bud transferred to carries out shoot proliferation on the subculture medium and cultivate.When March, carry out cuttage, select red heart soil as matrix, pulverizer is pulverized the back and is crossed 90～100 mesh sieves; In the plastic cup of the interior 10cm deeply of the 7 * 9cm that packs into; Place on the bed of growing seedlings, before the cuttage with the disinfecting solution of potassium permanganate 36h of 5g/L, sterilization back clear water drench matrix and cleaning medicine; Select robust growth, the subculture bud of height >=5cm is as the fringe bar, the flush away medium; After the 3mm place shears under the joint endways, fringe bar end is immersed in the MS liquid nutrient medium that contains 0.4mg/L ABT6, soaks part and account for 1/5～1/4 of whole fringe bar length; Flushing with clean water behind the immersion treatment 50min, in the vertical matrix cup that inserts after disinfecting, insertion depth accounts for 1/4～1/3 of whole fringe bar; After cuttage cuttage on the same day finishes, the clear water matrix of drenching, the high hut of preserving moisture of 60cm is built with the white plastic film in the top; Cover the shading screen of degree of shading 75%～85% again, temperature of shed is controlled at 15 ℃～35 ℃, and humidity is controlled at 80%～90%; In time trickle keeps the skin wet, and temperature is too high, and to raise film aeration-cooling.After growing seedlings 20 days, but raise film ventilation 40min every day.After growing seedlings 18 days, cuttage seeding root survival 93%.Cuttage seeding continues timely trickle between culture period, weeding, fertilising and the attention extermination of disease and insect pest, and more than the height of seedling 50cm, no damage by disease and insect can go out garden afforestation.
Embodiment 3:
The spray that has axillalry bud of selecting the peripheral middle and upper part of healthy and strong disease-free beans plant tree crown to give birth to then is an explant, and sterilization is seeded to aseptic culture medium and carries out the bud inducing culture, obtain aseptic bud after, aseptic bud transferred to carries out shoot proliferation on the subculture medium and cultivate.When April, carry out cuttage, select red heart soil as matrix, pulverizer is pulverized the back and is crossed 80～100 mesh sieves; In the plastic cup of the interior 10cm deeply of the 7 * 9cm that packs into; Place on the bed of growing seedlings, before the cuttage with the disinfecting solution of potassium permanganate 24h of 5g/L, sterilization back clear water drench matrix and cleaning medicine; Select robust growth, the subculture bud of height >=5cm is as the fringe bar, the flush away medium; After the 3mm place shears under the joint endways, fringe bar end is immersed in the MS liquid nutrient medium that contains 0.5mg/L ABT6, soaks part and account for 1/5～1/4 of whole fringe bar length; Flushing with clean water behind the immersion treatment 50min, in the vertical matrix cup that inserts after disinfecting, insertion depth accounts for 1/4～1/3 of whole fringe bar; After cuttage cuttage on the same day finishes, the clear water matrix of drenching, the high hut of preserving moisture of 60cm is built with the white plastic film in the top; Cover the shading screen of degree of shading 75%～85% again, temperature of shed is controlled at 15 ℃～35 ℃, and humidity is controlled at 80%～90%; In time trickle keeps the skin wet, and temperature is too high, and to raise film aeration-cooling.After growing seedlings 20 days, but raise film ventilation 60min every day.After growing seedlings 15 days, cuttage seeding root survival 91%.Cuttage seeding continues timely trickle between culture period, weeding, fertilising and the attention extermination of disease and insect pest, and more than the height of seedling 50cm, no damage by disease and insect can go out garden afforestation.
Embodiment 4:
The spray that has axillalry bud of selecting the peripheral middle and upper part of healthy and strong disease-free beans plant tree crown to give birth to then is an explant, and sterilization is seeded to aseptic culture medium and carries out the bud inducing culture, obtain aseptic bud after, aseptic bud transferred to carries out shoot proliferation on the subculture medium and cultivate.When December, carry out cuttage, select red heart soil as matrix, pulverizer is pulverized the back and is crossed 80～100 mesh sieves; In the plastic cup of the interior 10cm deeply of the 7 * 9cm that packs into; Place on the bed of growing seedlings, before the cuttage with the disinfecting solution of potassium permanganate 36h of 5g/L, sterilization back clear water drench matrix and cleaning medicine; Select robust growth, the subculture bud of height >=5cm is as the fringe bar, the flush away medium; After the 3mm place shears under the joint endways, fringe bar end is immersed in the MS liquid nutrient medium that contains 0.5mg/L ABT6, soaks part and account for 1/5～1/4 of whole fringe bar length; Flushing with clean water behind the immersion treatment 50min, in the vertical matrix cup that inserts after disinfecting, insertion depth accounts for 1/4～1/3 of whole fringe bar; After cuttage cuttage on the same day finishes, the clear water matrix of drenching, the high hut of preserving moisture of 60cm is built with the white plastic film in the top; Cover the shading screen of degree of shading 75%～85% again, temperature of shed is controlled at 15 ℃～35 ℃, and humidity is controlled at 80%～90%; In time trickle keeps the skin wet, and temperature is too high, and to raise film aeration-cooling.After growing seedlings 25 days, but raise film ventilation 40min every day, after growing seedlings 20 days, cuttage seeding root survival 85%.Cuttage seeding continues timely trickle between culture period, weeding, fertilising and the attention extermination of disease and insect pest, and more than the height of seedling 50cm, no damage by disease and insect can go out garden afforestation.
Claims (3)
1. appoint the outer cuttage and seedling culture method of beans group training subculture bud bottle for one kind; Comprise and appoint the cultivation of beans group training subculture bud, matrix preparation, cuttage, grow seedlings and go out 5 steps in garden, it is characterized in that: the method that employing group training subculture bud outside sprout-cultivating-bottle and two steps of cuttage and seedling culture unite two into one, appoint beans group training subculture bud with the conventional method tissue culture propagation earlier; Choose again and appoint beans group training subculture bud as inserting fringe; In rooting promoter processing and cuttage to red heart soil matrix matter, to grow seedlings and cultivate the wooden seedling that obtains afforesting, its operating procedure is following:
(1) group training subculture bud is cultivated: the spray that has axillalry bud of selecting the peripheral middle and upper part of healthy and strong disease-free beans plant tree crown to give birth to then is an explant; Sterilization is seeded to aseptic culture medium and carries out the bud inducing culture; After obtaining aseptic bud, aseptic bud transferred to carry out enrichment culture on the subculture medium;
(2) matrix is prepared: select red heart soil as matrix; Pulverize the back with pulverizer and cross 80～100 mesh sieves; In the plastic cup of the interior 10cm deeply of the 7 * 9cm that packs into; Place on the bed of growing seedlings, with disinfecting solution of potassium permanganate 24～36h of 3g/L～5g/L, clear water drench matrix and cleaning medicine in sterilization back is subsequent use before the cuttage;
(3) cuttage: the subculture bud of clip robust growth is as the fringe bar; After fringe bar shearing end handled with rooting promoter, vertically insert in the matrix of plastic cup, insertion depth accounts for 1/4～1/3 of whole fringe bar; With the clear water matrix of drenching, be May September to next year then in cuttage season after the cuttage;
(4) grow seedlings: the high hut of preserving moisture of 50～70cm is built with the white plastic film in the bed top of growing seedlings, and covers the shading screen of degree of shading 75%～85% again, presses conventional method trickle, weeding, fertilising and the extermination of disease and insect pest during the cultivation cuttage seeding;
(5) go out the garden: more than the height of seedling 50cm, no damage by disease and insect goes out garden afforestation.
2. the outer cuttage and seedling culture method of a kind of beans group training subculture bud bottle according to claim 1, it is characterized in that: the described fringe bar of step (3) is a subculture bud of selecting height >=5cm, the flush away medium, and joint is sheared at the 3mm place down endways.
3. the outer cuttage and seedling culture method of a kind of beans group training subculture bud bottle according to claim 1; It is characterized in that: it is that fringe bar shearing end is immersed in the MS liquid nutrient medium that contains 0.3mg/L～0.5mg/L ABT6 that the described rooting promoter of step (3) is handled; Soak part and account for 1/5～1/4 of whole fringe bar length, flushing with clean water is handled and is used for cuttage behind immersion treatment 40～60min.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103518600A (en) * | 2013-10-15 | 2014-01-22 | 中国长江三峡集团公司 | Method of outdoor hydroponic rooting of ficus elastica test-tube plantlets |
| CN104145793A (en) * | 2014-08-08 | 2014-11-19 | 广西壮族自治区林业科学研究院 | Root-cutting seedling method of zenia insignis |
| CN104737766A (en) * | 2015-04-13 | 2015-07-01 | 成都市三禾田生物技术有限公司 | Method for artificially cultivating resveratrol-enhanced polygonum cuspidatum through tissue culture |
| CN105009908A (en) * | 2015-07-29 | 2015-11-04 | 广西壮族自治区林业科学研究院 | Out-bottle one-step plantlet developing method for rootless test-tube cunninghamia lanceolata seedlings |
| CN106305416A (en) * | 2016-08-12 | 2017-01-11 | 华南农业大学 | Efficient regeneration method with zenia insignis leaves being explants |
| CN109997537A (en) * | 2019-05-21 | 2019-07-12 | 黑龙江伊蓝生物科技有限公司 | The micro- plug bottle ex vitro rooting technique of indigo fruit tissue culture subculture unrooted test tube seedling |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411282A (en) * | 2008-11-14 | 2009-04-22 | 中国科学院亚热带农业生态研究所 | Method for cultivating flood-refuge crops in hollow of southwest karst peak cluster |
-
2012
- 2012-09-18 CN CN 201210345948 patent/CN102823414B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411282A (en) * | 2008-11-14 | 2009-04-22 | 中国科学院亚热带农业生态研究所 | Method for cultivating flood-refuge crops in hollow of southwest karst peak cluster |
Non-Patent Citations (2)
| Title |
|---|
| 童方平; 龚树立; 邓德明; 李贵; 刘振华; 童琪: "翅荚木优质苗木培育技术", 《湖南林业科技》 * |
| 莫汉宁: "石灰岩地区香椿、任豆速丰林混交种植技术研究", 《吉林农业》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103518600A (en) * | 2013-10-15 | 2014-01-22 | 中国长江三峡集团公司 | Method of outdoor hydroponic rooting of ficus elastica test-tube plantlets |
| CN104145793A (en) * | 2014-08-08 | 2014-11-19 | 广西壮族自治区林业科学研究院 | Root-cutting seedling method of zenia insignis |
| CN104145793B (en) * | 2014-08-08 | 2016-02-17 | 广西壮族自治区林业科学研究院 | The root-cut breeding method of a kind of beans |
| CN104737766A (en) * | 2015-04-13 | 2015-07-01 | 成都市三禾田生物技术有限公司 | Method for artificially cultivating resveratrol-enhanced polygonum cuspidatum through tissue culture |
| CN105009908A (en) * | 2015-07-29 | 2015-11-04 | 广西壮族自治区林业科学研究院 | Out-bottle one-step plantlet developing method for rootless test-tube cunninghamia lanceolata seedlings |
| CN105009908B (en) * | 2015-07-29 | 2017-11-24 | 广西壮族自治区林业科学研究院 | The method of the outer forming seedling through one step culture of China fir unrooted test tube seedling bottle |
| CN106305416A (en) * | 2016-08-12 | 2017-01-11 | 华南农业大学 | Efficient regeneration method with zenia insignis leaves being explants |
| CN106305416B (en) * | 2016-08-12 | 2018-07-27 | 华南农业大学 | It is a kind of using appoint beans blade as the highly efficient regeneration method of explant |
| CN109997537A (en) * | 2019-05-21 | 2019-07-12 | 黑龙江伊蓝生物科技有限公司 | The micro- plug bottle ex vitro rooting technique of indigo fruit tissue culture subculture unrooted test tube seedling |
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| CN102823414B (en) | 2013-10-02 |
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