CN103518600A - Method of outdoor hydroponic rooting of ficus elastica test-tube plantlets - Google Patents
Method of outdoor hydroponic rooting of ficus elastica test-tube plantlets Download PDFInfo
- Publication number
- CN103518600A CN103518600A CN201310480086.8A CN201310480086A CN103518600A CN 103518600 A CN103518600 A CN 103518600A CN 201310480086 A CN201310480086 A CN 201310480086A CN 103518600 A CN103518600 A CN 103518600A
- Authority
- CN
- China
- Prior art keywords
- test
- india
- rubber
- tube plantlet
- rooting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- Y02P60/216—
Abstract
The invention provides a method of outdoor hydroponic rooting of ficus elastica test-tube plantlets. According to the method, rooting induction and seedling hardening are completed in one step, specifically, tap water is supplemented in time when a nutrient solution is at a level lower than one third of the stem of each ficus elastica test-tube plantlet in a square plate, auxin IBA in the nutrient solution of the ficus elastica test-tube plantlets is replaced every 3-7 days, and the concentration of the nutrient solution is kept unchanged in the test process. Compared with a conventional tissue culture rooting method, the ficus elastica outdoor hydroponic rooting method enables the rooting period to be shortened by about 20 days. The work of culture medium preparation, indoor sterile inoculation, culture in a culture room, hardened seedling transplantation and the like can be eliminated for the ficus elastica test-tube plantlets by means of the hydroponic rooting method.
Description
Technical field
The present invention relates to the method that the outdoor water planting of a kind of India-rubber fig test-tube plantlet is taken root, root induction and hardening one step are completed, reduce in laboratory the processes such as strong sprout, root induction and outdoor hardening.
Background technology
The evergreen resistance to shady woody plant of India-rubber fig Moraceae Ficus, originates in India and Malaysia, and generous because of its keratin blade, ornamental value is high, very popular, is famous indoor sight leaf and Landscape Trees.Indoor the taking root of India-rubber fig will be passed through the work such as strong seedling culture, root induction and acclimatization and transplants, and root induction need carry out the conventional programs such as medium preparation, sterilizing, inoculation and culturing room's cultivation; Acclimatization and transplants also needs uncork hardening and cleans plant root medium, works loaded down with trivial details and easily root is caused to damage influence group training transplantation of seedlings survival rate.
Summary of the invention
The method that the object of the present invention is to provide the outdoor water planting of a kind of India-rubber fig test-tube plantlet to take root, the India-rubber fig test-tube plantlet of 3.5-5.5cm of take is test material, adopt IBA growth hormone to process under 0-1.5mg/L, and transplant, overcome in prior art in rooting of vitro seedling induction and acclimatization and transplants process working procedure complicated, the shortcoming such as the large and production cost of workload is high.
The present invention realizes above-mentioned purpose like this: adopt through continuous 6 subcultures and cultivate India-rubber fig in vitro cuttings.First India-rubber fig test-tube plantlet directly being taken out to clean medium in blake bottle, put into the Stainless steel basin being filled with water, then from India-rubber fig test-tube plantlet callus, cutting specification is 3.5 ~ 5.5cm individual plant India-rubber fig test-tube plantlet, break except radical leaves and clean the juice flowing out, again individual plant test-tube plantlet is inserted in back-off 72 dish holes, hole and puts into the square plate that is filled with water, carry out outdoor water culture experiment.The water yield is controlled at and does not contact blade and be as the criterion in square plate, the transplantation of seedlings of finally water planting being taken root.
In outdoor water planting culture of rootage, India-rubber fig test-tube plantlet is carried out to four processing, three and repeat the medium root induction of the outdoor water planting root induction test of single-factor ，Bing Yi culturing room and compare.The concrete grammar of water planting root induction is: according to the running water water yield of evaporating in how many in time supplementary culture fluids of temperature height and the square plate water yield that is filled with water,, when culture fluid lower than square plate in India-rubber fig test-tube plantlet stem section 1/3 time, supplement in time running water, and the culture fluid growth hormone IBA of India-rubber fig test-tube plantlet was changed in 3-7 days, keep IBA concentration and experimental scheme concentration constant, guarantee water cleaning.
The concentration of described growth hormone IBA is 0 ~ 1.5mg/L.
After water planting 30-40 days, India-rubber fig test-tube plantlet is transplanted, the environment facies that the growing environment of transplanted seedling and India-rubber fig water planting are taken root are same, and wherein transplanting medium is that garden mould and vermiculite be take mass ratio as 2:1 mixing.
The invention has the advantages that the outdoor water planting culture of rootage of India-rubber fig test-tube plantlet method is simple, root induction and hardening one step are completed, reduce in laboratory the processes such as strong sprout, root induction and outdoor hardening, improved reproduction speed, strengthened the ability of test-tube plantlet reform of nature environment.
The outdoor water planting of India-rubber fig test-tube plantlet take root solved that group training takes root need be in gnotobasis to open culture of rootage conversion in natural environment, thoroughly solved the difficult problem that sterile working is easily polluted, greatly reduced staff's labour intensity, save production cost, and can reach the object of factorial seedling growth.
The outdoor water planting of India-rubber fig is taken root and is taken root and shorten about 20 days than the training of routine group.India-rubber fig test-tube plantlet is taken root and can have been reduced that medium preparation, indoor aseptic inoculation, culturing room are cultivated and the work such as acclimatization and transplants by water planting.
Accompanying drawing explanation
Fig. 1,2,3,4 is that indoor group of India-rubber fig is trained the situation of taking root.
Fig. 6,7,8,9 is the culture fluid IBA concentration outdoor water planting of India-rubber fig situation of taking root while being respectively 1.5mg/L, 1.0 mg/L, 0.5 mg/L, 0 mg/L.
Fig. 5 is that India-rubber fig Rooted Cuttings is selected specification, is specially 3.5-5.5cm.
Figure 10 is culture fluid IBA concentration India-rubber fig water planting rooting and transplant situation while being 0mg/L.
Embodiment
embodiment 1
India-rubber fig test-tube plantlet is carried out to four processing, the outdoor water planting root induction test of three repeated growth elements concentration single-factors, and wherein auxin concentration is that 1.5mg/L, 1.0 mg/L, 0.5 mg/L, the medium root induction of 0 mg/L ，Bing Yi culturing room compare.According to the running water water yield of evaporating in how many in time supplementary culture fluids of temperature height and the square plate water yield that is filled with water, when culture fluid lower than square plate in India-rubber fig test-tube plantlet stem section 1/3 time, supplement in time running water, and every 3-7 changes water in four processing, keep IBA concentration and experimental scheme concentration constant, guarantee water cleaning.
Through cultivation in 2-3 days, insert containing test-tube plantlet stem section in different I BA concentration solution and start to occur white point, form root base; Within 5-6 days, from root base director, go out one-level root, and carry out elongation growth gradually, length is 0.3-0.5cm.Through the outdoor rooting of vitro seedling induction of India-rubber fig in 30 days, cultivate data statistics, its average rooting rate is 93.33%, 95.78%, 97.33%, 100%; The length of on average taking root is respectively 3.19 cm, 3.44 cm, 4.29cm, 5.12cm; The number of on average taking root is respectively 9.35,8.2,5.73,4.78 (seeing Fig. 6,7,8,9).The training of indoor group of India-rubber fig is taken root, and rooting rate reaches 100% to mode (seeing Fig. 1,2,3,4), and outdoor water planting rooting rate also can reach 100%, but composite factor considers that tool has great advantage from simplifying procedures, reduce workload and reduce production costs etc.
After water planting 30-40 days, India-rubber fig test-tube plantlet is transplanted, the environment facies that the growing environment of transplanted seedling and India-rubber fig water planting are taken root are same, and wherein transplanting medium is that garden mould and vermiculite be take mass ratio as 2:1 mixing.To India-rubber fig water planting, the transplantation of seedlings of taking root is carried out transplanting survival rate statistical analysis and is shown after 3 weeks: four kinds of different I BA concentration that India-rubber fig test-tube plantlet water planting is taken root in experiment are processed (1.5mg/L, 1.0 mg/L, 0.5 mg/L, 0 mg/L) transplanting survival rates and are respectively 70.38%, 81.60%, 85.85%, 90.71%, when IBA concentration is 0mg/L, India-rubber fig transplanting survival rate maximum has reached more than 90% desirable level.Its growth cycle shortens about 20 days than tissue culture mode.Because the India-rubber fig water planting root that seedling produces of taking root is meat fibrous root, will be in strict accordance with the principle of " dry do not water, water and irrigate " during final management and maintenance, otherwise easily cause that root and stem section rot.
Claims (3)
1. the method that the outdoor water planting of India-rubber fig test-tube plantlet is taken root, adopt root induction and hardening one step to complete, India-rubber fig test-tube plantlet is directly taken out in blake bottle, after cleaning medium, put into the Stainless steel basin being filled with water, from India-rubber fig test-tube plantlet callus, cutting specification is 3.5 ~ 5.5cm individual plant India-rubber fig test-tube plantlet, break except radical leaves and clean the juice flowing out, again individual plant test-tube plantlet is inserted in back-off 72 dish holes, hole and puts into the square plate that is filled with water, carry out outdoor water culture experiment, in square plate, the water yield is controlled at and does not contact blade, the transplantation of seedlings of finally water planting being taken root, it is characterized in that: when culture fluid lower than square plate in India-rubber fig test-tube plantlet stem section 1/3 time, supplement in time running water, the culture fluid growth hormone IBA of India-rubber fig test-tube plantlet was changed in 3 ~ 7 days, and in keeping testing, culture fluid concentration is constant.
2. the method that the outdoor water planting of India-rubber fig test-tube plantlet according to claim 1 is taken root, is characterized in that: the concentration of growth hormone IBA is 0 ~ 1.5mg/L.
3. the method that the outdoor water planting of India-rubber fig test-tube plantlet according to claim 1 is taken root, it is characterized in that: after water planting 30-40 days, India-rubber fig test-tube plantlet is transplanted, the environment facies that the growing environment of transplanted seedling and India-rubber fig water planting are taken root are same, and wherein transplanting medium is that garden mould and vermiculite be take mass ratio as 2:1 mixing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310480086.8A CN103518600B (en) | 2013-10-15 | 2013-10-15 | Method of outdoor hydroponic rooting of ficus elastica test-tube plantlets |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310480086.8A CN103518600B (en) | 2013-10-15 | 2013-10-15 | Method of outdoor hydroponic rooting of ficus elastica test-tube plantlets |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103518600A true CN103518600A (en) | 2014-01-22 |
| CN103518600B CN103518600B (en) | 2015-02-18 |
Family
ID=49921243
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310480086.8A Active CN103518600B (en) | 2013-10-15 | 2013-10-15 | Method of outdoor hydroponic rooting of ficus elastica test-tube plantlets |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103518600B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638419A (en) * | 2016-03-16 | 2016-06-08 | 蒋凡 | Processing method for anti-long-distance transportation of eucalyptus robusta Smith tissue culture seedlings |
| CN109042215A (en) * | 2018-09-07 | 2018-12-21 | 广西生态工程职业技术学院 | A kind of processing method of the anti-long-distance transport of Eucalyptus Tissue Cultured bottle seedling |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631089A (en) * | 2004-12-24 | 2005-06-29 | 中国科学院武汉植物园 | Culture method of Sinningia speciosa |
| CN102823414A (en) * | 2012-09-18 | 2012-12-19 | 广西壮族自治区林业科学研究院 | Non-tube cutting seedling method of tissue subcultured buds of zenia |
| CN103053416A (en) * | 2012-12-26 | 2013-04-24 | 中国长江三峡集团公司 | Method for tissue culture and rapid propagation of ficus elastica |
-
2013
- 2013-10-15 CN CN201310480086.8A patent/CN103518600B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1631089A (en) * | 2004-12-24 | 2005-06-29 | 中国科学院武汉植物园 | Culture method of Sinningia speciosa |
| CN102823414A (en) * | 2012-09-18 | 2012-12-19 | 广西壮族自治区林业科学研究院 | Non-tube cutting seedling method of tissue subcultured buds of zenia |
| CN103053416A (en) * | 2012-12-26 | 2013-04-24 | 中国长江三峡集团公司 | Method for tissue culture and rapid propagation of ficus elastica |
Non-Patent Citations (5)
| Title |
|---|
| 徐振华等: "试管苗瓶外生根的研究进展", 《中国农学通报》 * |
| 陶秀冬等: "橡皮树组织培养及快繁技术研究", 《新疆农业科学》 * |
| 颜昌敬: "《农作物组织培养》", 28 February 1991 * |
| 颜昌敬等: "苎麻试管苗带菌水培生根技术研究", 《中国农业科学》 * |
| 黄卓忠等: "试管苗瓶外生根技术", 《广西农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638419A (en) * | 2016-03-16 | 2016-06-08 | 蒋凡 | Processing method for anti-long-distance transportation of eucalyptus robusta Smith tissue culture seedlings |
| CN109042215A (en) * | 2018-09-07 | 2018-12-21 | 广西生态工程职业技术学院 | A kind of processing method of the anti-long-distance transport of Eucalyptus Tissue Cultured bottle seedling |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103518600B (en) | 2015-02-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102150624B (en) | Tissue culture and rapid propagation method for pinellia tuber plant | |
| CN105052737B (en) | A kind of tissue culture method of P. kingianum seed forming seedling through one step culture | |
| CN103931492A (en) | Tissue-culture rapid seedling growing method for apple rootstock M9 | |
| CN106576910B (en) | Culture method of in-vitro sterile host plant-mycorrhizal edible fungus symbiotic seedling | |
| CN101116424B (en) | Highly effective lily bulblet inducement culture method | |
| CN104885773A (en) | Method for rapidly cultivating early shaping tissue culture commodity seedlings of blueberries | |
| CN101926287A (en) | Method for culturing tissue of 'Zhongzhen No.1' | |
| CN107018896B (en) | A kind of method of facility cuttage tilia miqueliana | |
| CN104823824A (en) | Temporary immersion type culture method and culture device for dendrobe | |
| CN105265320B (en) | A kind of tissue culture propagation of aristolochia mollissima | |
| CN104137779A (en) | Method for regenerating sapium japonicum plant by inducing sapium japonicum stem rapidly | |
| CN110786157A (en) | Method for promoting rooting of ilex verticillata tissue culture seedlings | |
| CN102217538B (en) | Method for treating walnut tissue culture stem segments inside test tubes and rooting outside test tubes | |
| CN106818468A (en) | A kind of shellflower seed asepsis sprouting and rapid propagation method | |
| CN103283504B (en) | Method for grafting pear polyploidy test-tube plantlet outside test tube | |
| CN110663552B (en) | Tissue culture and rapid propagation method of Yunnan tung tree | |
| CN103518600B (en) | Method of outdoor hydroponic rooting of ficus elastica test-tube plantlets | |
| CN104904601B (en) | The method that the aseptic brachyplast of medicinal Rhizoma Dioscoreae Zingiberensiss tissue cultured seedling takes root | |
| CN103609444A (en) | Tissue culture method for hemerocallis sempervirens araki | |
| CN102524012A (en) | Exercising and transplanting method for fruit tree tissue culture seedlings | |
| CN102487801A (en) | Method for soilless culture of bulblets of tissue culture seedlings of oriental lily | |
| CN106857265B (en) | A kind of tissue culture enrichment procedure of white Cowhells wood | |
| CN101766086B (en) | Method for rapidly cutting and reproducing euphorbia pekinensis | |
| CN104920215B (en) | The sugarcane rooting of vitro seedling and method for culturing seedlings of a kind of simplification | |
| CN104488726B (en) | The outside sprout-cultivating-bottle method of radix pseudostellariae test tube seedling |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Ma Xiaobo Inventor after: Zhang Guoyu Inventor after: Huang Guiyun Inventor after: Qiu Liwen Inventor after: Hu Meixiang Inventor after: Wang Lei Inventor after: Wu Di Inventor after: Yang Lanfang Inventor after: Zhang Haibo Inventor before: Ma Xiaobo Inventor before: Zhang Guoyu Inventor before: Huang Guiyun |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: MA XIAOBO ZHANG GUOYU HUANG GUIYUN TO: MA XIAOBO ZHANG GUOYU HUANG GUIYUN QIU LIWEN HU MEIXIANG WANG LEI WU DI YANG LANFANG ZHANG HAIBO |