CN101766086B

CN101766086B - Method for rapidly cutting and reproducing euphorbia pekinensis

Info

Publication number: CN101766086B
Application number: CN200910114804A
Authority: CN
Inventors: 李培栋; 戴传超; 王兴祥; 周静
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-01-04
Filing date: 2009-01-04
Publication date: 2012-09-12
Anticipated expiration: 2029-01-04
Also published as: CN101766086A

Abstract

The invention provides a method for rapidly cutting and reproducing euphorbia pekinensis, comprising the processes of cutting shoot selection, matrix preparation, cutting, water and manure management and the like; wherein the length of cutting branches is 8-12cm; the cutting branches and the matrix are sprayed by using drugs before cutting; and the fertilization and drug spraying treatment is carried out after cutting. The invention has the characteristics of simple process, high survival rate and the like.

Description

Method for rapidly cutting and reproducing euphorbia pekinensis

Technical field

The present invention relates to field of plant growing technology.Relate in particular to a kind of method for rapidly cutting and reproducing euphorbia pekinensis.

Technical background

The root of Beijing euphorbia (being commonly called as the Beijing spurge root, Euphorbia pekinensis Ruprecht) is the Euphorbiaceae euphorbia, is the folks of china medicinal herbs most in use, records in successive dynasties Chinese medicine pharmacopeia.Under field conditions (factors), root of Beijing euphorbia seed amount is few, vitality and germination rate are low, the multifactor restriction of breeding audient, and also growth is slow, needs cultivation for many years, therefore is necessary to develop a kind of method for quickly breeding of the root of Beijing euphorbia, to satisfy the health of people needs and the market demand.The propagation method of the existing root of Beijing euphorbia has seminal propagation method, root division method, tissue culture method and cottage propagation method.Seminal propagation is owned by France in traditional propagation method, and is limited but seed amount is produced in the root of Beijing euphorbia, and germinate and receive the restriction of numerous factors, and its reproduction rate is lower, and the breeding cycle of the method is long, adopts seldom.Though root division method survival rate is very high, the annual chance of breeding for once, and do not have enough piece roots to cut apart breeding, therefore be unfavorable for a large amount of breedings fast.Tissue culture method is as a kind of new technology; Utilize the totipotency of plant cell under aseptic and artificial control nutrition and environmental condition, organ, tissue or the cell of medicinal plant to be cultivated, be used for producing medicinal active ingredient or carry out the asexual quick breeding of medicinal plant.Root of Beijing euphorbia tissue culture mainly is to adopt stem apex to carry out, and generally adopts the method breeding root of Beijing euphorbia of stem apex evoked callus, and rooting rate is 47.1%, but the callus germination rate that utilizes stem apex to induce is merely 12%, and refines the easy microbiological contamination of seedling stage and death.Though adopt root of Beijing euphorbia endophyte can improve the refining shoot survival percent of root of Beijing euphorbia tissue cultivating seedling, the method for tissue culture all than higher, is difficult to large-scale application to the requirement of production facility and professional skill and production cost.The cottage propagation method has had the history of more than one thousand years, and offspring's aberration rate of its breeding is low, proterties stable, can keep the biological nature in female generation well, guarantees the quality of medicinal plant, is well suited for the breeding of medicinal plant.In the cottage propagation measure of the existing root of Beijing euphorbia, adopting more at present is stem apex cottage propagation method, and survival rate can be up to 88.6%; But the limited amount in the stem apex of root of Beijing euphorbia source; The velocity ratio that sprout tillers produces new stem apex is slower, is difficult to carry out a large amount of breedings, has limited promoting the use of of this method; Therefore be necessary to enlarge the scope of its slotting fringe, to improve proliferative speed.The stem stalk also is good slotting fringe source, but conventional method utilizes the stem stalk to insert the cuttage of fringe field at present, and its survival rate often is no more than 5%.

Summary of the invention

The object of the invention is exactly to insert the low technical barrier of fringe survival rate in order to solve present stem stalk, and a kind of method for rapidly cutting and reproducing euphorbia pekinensis is provided.This method can make the survival rate of the cuttage root of Beijing euphorbia up to more than 60%.

Concrete operations technology is following:

1, slotting ear selection is selected with the cuttage branch and handled: this method adopts the stem stalk of the root of Beijing euphorbia to carry out cottage propagation, enlarged the scope of slotting fringe, and root of Beijing euphorbia stem stalk growth fraction is very fast, is easy to repeat to obtain, and the root of Beijing euphorbia of cutting survival also can be used as the source of inserting fringe.Branch on the root of Beijing euphorbia plant that clip grows fine; Be cut into the cuttage branch of length 8-12cm; Cuttage branch bottom is preferably in the bottom intercepting near tubercle, cuts the leaf on the cuttage branch, only keeps end of the branch 1-2 sheet leaf and gets final product (noting not selecting the serious old branch of lignification as the cuttage branch).The lower end 1cm of the cuttage branch that shears was immersed in the root-inducing powder naa solution 10-15 minute; Said root-inducing powder naa solution is dissolved in 1.6 premium on currency formulated by the 10g root-inducing powder; Spray carbendazim solution to soaking good cuttage branch bottom again, place and carry out cuttage after 5 minutes.

2, matrix preparation: get than fertile soil, spread out on concrete floor, be exposed to the sun under the sun 2-3 days, to kill the pathogen in the soil.Prepare vermiculite, the peat composed of rotten mosses and the perlite of fresh dried, in 1: 1: 1 ratio mixing crop matrix, matrix was for join existing usefulness at present.Soil and matrix are adorned basin with 1: 2 volume, and soil is in flowerpot lower floor, and matrix is on the upper strata, and the volume of specifically packing into depends on the size of flowerpot.The general hypothallus thickness that guarantees is greater than 5cm.

3, cuttage technique: spray the matrix in the flowerpot with carbendazim (1g/L) earlier; There are flowing out until basin bottom part, on matrix, stab a hole then, be about 1-2cm deeply; Compacting gently after the cuttage stem cutting inside of the root of Beijing euphorbia, a small amount of carbendazim solution is sprayed to the root of Beijing euphorbia in the intact back of cuttage.Cutting density is generally with 1-2cm ²1 strain is advisable.

4, water and fertilizer management: the flowerpot of the good root of Beijing euphorbia of cuttage is placed on sunshade, ventilation, shady and cool place, and temperature is good at 25 ℃-30 ℃.In 1 month after cuttage, spray to keep water content of substrate about 60% with carbendazim solution, urea liquid, superphosphate solution and Klorvess Liquid.After 1 month, reduce the sprinkling amount of carbendazim solution, the water content that keeps matrix is about 40%, in order to the growth of queen's delight.Just can root of Beijing euphorbia seedling be carried out field transplanting after 2 months.The method is applicable to that also the field of sunshade net carries out cuttage.

The present invention has characteristics such as technology is simple, survival rate height.

Claims

1. method for rapidly cutting and reproducing euphorbia pekinensis, concrete operations technology is following:

(1) slotting ear selection is selected with the cuttage branch and is handled: branch on the root of Beijing euphorbia plant that clip grows fine; Be cut into the cuttage branch of length 8-12cm; The leaf on the cuttage branch is cut in the bottom intercepting near tubercle in cuttage branch bottom, only keeps end of the branch 1-2 sheet leaf and gets final product; The lower end 1cm of the cuttage branch that shears was immersed in the root-inducing powder naa solution 10-15 minute; Said root-inducing powder naa solution is dissolved in 1.6 premium on currency formulated by the 10g root-inducing powder, spray carbendazim solution to soaking good cuttage branch bottom again, places and carries out cuttage after 5 minutes;

(2) matrix preparation: get than fertile soil, spread out on concrete floor, be exposed to the sun under the sun 2-3 days; To kill the pathogen in the soil, prepare vermiculite, the peat composed of rotten mosses and the perlite of fresh dried, in 1: 1: 1 ratio mixing crop matrix; Soil and matrix are adorned basin with 1: 2 volume; Soil is in flowerpot lower floor, and matrix guarantees that on the upper strata hypothallus thickness is greater than 5cm;

(3) cuttage technique: spray the matrix in the flowerpot with carbendazim solution earlier, there are flowing out, on matrix, stab a hole then until basin bottom part; Dark is 1-2cm; Compacting gently after the cuttage stem cutting inside of the root of Beijing euphorbia, a small amount of carbendazim solution is sprayed to the root of Beijing euphorbia in the intact back of cuttage, and cutting density is generally with 1-2cm ²1 strain is advisable;

(4) water and fertilizer management: the flowerpot of the good root of Beijing euphorbia of cuttage is placed on sunshade, ventilation, shady and cool place; Temperature is at 25 ℃-30 ℃; In 1 month after cuttage, spray to keep water content of substrate about 60%, after 1 month with carbendazim solution, urea liquid, superphosphate solution and Klorvess Liquid; Reduce the sprinkling amount of carbendazim solution; The water content that keeps matrix just can be carried out field transplanting with root of Beijing euphorbia seedling after 2 months about 40%, said carbendazim solution is to be dissolved in 1 premium on currency formulated by the 1g carbendazim; Said urea liquid is to be dissolved in 1 premium on currency formulated by 1g urea; Said superphosphate solution is to be dissolved in 1 premium on currency formulated by 1g superphosphate; Said Klorvess Liquid is to be dissolved in 1 premium on currency formulated by 1g potassium chloride.